Laboratory diagnosis of bacteria causing respiratory diseases in chicken
Julian - Lab Diagnosis of Bacteria
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Transcript of Julian - Lab Diagnosis of Bacteria
Lab Diagnosis of Bacteria causing gastroenteritis/diarrhea
Staphylococcus aureus• Gram positive cocci in
grape like clusters taken from blood
• Golden yellow colonies in blood agar
Clostridium perfringens
-Nagler’s test on medium containing egg yolk-Opacity around the line of growth ( positive)
-Double zone hemolysisBeta-lysis and Alpha lysis
-Gram positive , spore forming rods
Campylobacter jejuni
• Gram negative bacilli taken from stool specimens
• Skirrow’s medium with antibiotics, reduced oxygen at 42 degree celcius .
Vibrio cholerae
• Gram negative, comma shaped bacilli
• Yellow colony (sucrose fermenting) on TCBS (thiosulphate-citrate-bile-sucrose) agar
Escherichia coli
• Gram negative rods
• Coliform(lactose fermenters)
• Pink colony on MacConkey agar
Bacillus cereus
• Gram positive rods with central and oval spores
• Schaeffer-Fulton stain showing green mature spores
Salmonella enterica
• Gram negative rods
• Salmonella-Shigella agar showing pale colonies with black center
• Salmonella enterica on XLD agar( xylose-lysine-deoxycolate)
• Black colony, agar turned blue on Hektoen agar.