Jiří Kyslík,Terezie Svobodová, Simona Zimová

27. 07. 2011

Analysis of protoplasmic streaming in plant cell

Supervisor: Jan Novák

Aims • Definition of protoplasmic streaming • Formulate if the protoplasmic streaming is random• Effect of NaCl solutions on protoplasmic streaming • Speed of plasmolysis by osmosis • Prove of toxicity in the plant cells (CuSO4 ∙ 5H2O)

• Speed of chloroplasts on the cytoplasmic filaments • Space reconstruction of the elodea cell

Methods

• Preparing different sections • Microcinematography techniques • Microscope techniques (optical microscope, fluorescent

microscope) • Analysis in softwares (NIS elements, ImageJ, IC capture,

Obtahovač) • Statistical analysis (MS Excel)

Biomodel

Results

Areas of chloroplasts in NaCl solutions

Heights of chloroplasts in NaCl solutions

Changing of size of chloroplasts with growing concentration of NaCl solutions

n=4045

Speed of chloroplasts

Speed of chloroplasts in 0,03M NaCl solution

Plasmolysis in 0,1 M NaCl solution

Speed of plasmolysis in NaCl solutions

Influence of 3M solution of CuSO4.5H2O in plant cells

Conclusions

• Streaming in protoplasma is random movement• Movement is slower on cytoplasmatic filaments • Hypotonic and hypertonic solutions influence sizes of

the chloroplasts• Experimental verification of the theoretical model• mastery of methods and microcinematography

Resources

• Noburô Kamiya,, Physical aspects of protoplasmic streaming, University of Tokio, Iowa State College Press, 1956

• Noburô Kamiya, Hirochimi Nakajima and Shigemi Abe, Physiology of the Motive Force of protoplasmic streaming, Department of Biology, Faculty of Science, Osaka University, 1943

• W.Seifriz, Pathological changes in protoplasm, 1939• KYSLÍK , Jiří. Studium pohybu protoplasmy v buňkách Elodea sp. . Nové Hrady,

2011. 13 s. Seminární práce. Gymnázium, SOŠE a SOU, Kaplice.• http://www.wolframalpha.com/entities/species/g9/cf/x8/img/img-7.gif (modified)

Acknowledgment

• Supervisor: Jan Novák • Technical support: Ing. Vítězslav Březina, CSc.,

Ing. Petr Císař, Ph.D • Organisators of summer academical schools

and also sponsors

THANK YOU FOR YOUR ATTENTION