INTERPLAY BETWEEN CYPRINID HERPESVIRUS 3 (CYHV-3/KHV) AND

COMMON CARP BODY TEMPERATURE

KRZYSZTOF RAKUS JAGIELLONIAN UNIVERSITY IN KRAKOW

POLAND

FACULTY OF VETERINARY MEDICINE

UNIVERSITY OF LIEGE

PROF. ALAIN VANDERPLASSCHEN

IMMUNOLOGY-VACCINOLOGY

Homologous host-virus model

RESEARCH MODEL

Common carp CyHV-3/KHV

Live attenuated vaccine VACCINE AGAINST KOI HERPESVIRUS

KHV/CYHV-3

Koi herpesvirus (KHV) = Cyprinid herpesvirus 3 (CyHV-3)

Dead carp (>100,000) from Biwa Lake (Japan, 2004) (Matsui et al., 2008)

CYHV-3 AT THE WORLD

CYHV-3 AT THE WORLD

CYPRINID HERPESVIRUS 3

CLASSIFICATION

CeH

V-1

7

IcH

V-1

Sa

lHV

-1

Sa

lHV

-2

RaH

V-1

RaH

V-2

Os

HV

-1

HH

V-1

HH

V-3

C

eH

V-9

EH

V-1

E

HV

-4

Ga

HV

-2

Ga

HV

-1

HH

V-5

Mu

HV

-1

HH

V-6

HH

V-7

ElH

V-1

HH

V-4

HH

V-8

Bo

HV

-4

Bo

HV

-2

Bo

HV

-5

Bo

HV

-1

Alloherpesviridae Herpesviridae

Malacoherpesviridae

BL

HV

CyH

V-3

CyH

V-2

CyH

V-1

1000 MA

450 MA

200 MA

Order: Herpesvirales

Family: Alloherpesviridae

Genus: Cyprinivirus

Species: Cyprinid herpesvirus 3

100 nm

(Hedrick 2000)

Glycoprotein

Envelope

Tegument

Genome

Capsid

CyHV-3 Herpesvirus

CYPRINID HERPESVIRUS 3

MORPHOLOGY

• Linear, double stranded DNA

• 295 kb

• 155 ORF

• Genes encode proteins potentially involved in immune evasion mechanisms

ORF4 and ORF12 TNFR homologues

ORF16 G-protein coupled receptor

ORF134 IL-10 homologue

ORF112 Zalpha domain containing protein

ORF139 Poxvirus B22R homologue

CYPRINID HERPESVIRUS 3

GENOME

Common carp Koi carp

HOST SPECIES

KHV DISEASE

• Highly infective

• High mortality

Apathy

Hyperemia

Mucus hypersecretion

Gill necrosis

Herpetic lesions

- CyHV-3 replication in cell culture is restricted by temperature and is blocked at the non-permissive temperature of 30oC (Dishon et al., 2007).

- The disease caused by CyHV-3 occured when water temperature is between 18°C to 28°C (Michel et al., 2010).

MORTALITY NO MORTALITY

THE EFFECT OF TEMPERATURE ON CYHV-3

BEHAVIOURAL FEVER

In ectothermic animals, fever is based on a behavior modification leading the individual to reside in a warmer environment, hence is

named “behavioural fever”

WORKING HYPOTHESES

Do Common carp express behavioural fever in response to CyHV-3 infection?

And if so, is the expression of behavioural fever salutary for the carp?

MCT (multichamber tank) with temperature gradient

TANK SYSTEM

TANK SYSTEM

MCT (multichamber tank) with temperature gradient

TANK SYSTEM

MCT (multichamber tank) with temperature gradient

Axis of symmetry

DO COMMON CARP EXPRESS BEHAVIORAL FEVER IN RESPONSE TO CYHV-3 INFECTION?

Mock-infected CyHV-3-infected

15 fish 15 fish

DO COMMON CARP EXPRESS BEHAVIORAL FEVER IN RESPONSE TO CYHV-3 INFECTION?

Mock-infected CyHV-3-infected

COMMON CARP EXPRESS BEHAVIORAL FEVER IN RESPONSE

TO CYHV-3 INFECTION

COMMON CARP EXPRESS BEHAVIORAL FEVER IN RESPONSE TO CYHV-3 INFECTION

IS THE EXPRESSION OF BEHAVIORAL FEVER SALUTARY FOR COMMON CARP?

34°C 28°C 24°C

Survival analysis

SCT (ang. single chamber tanks)

MCT- blocked

32°C 28°C 24°C

THE EXPRESSION OF BEHAVIORAL FEVER IS SALUTARY FOR

COMMON CARP

IS THE EXPRESSION OF BEHAVIORAL FEVER SALUTARY FOR COMMON CARP?

Survival analysis

CLINICAL SIGNS OF FISH BLOCKED AT DIFFERENT TEMPERATURE

FEEDING

CONCLUSIONS

Carp express salutary behavioral fever in response to CyHV-3 infection

ACKNOWLEWDGMENTS Immunology and Vaccinology (FARAH-ULg) Alain Vanderplasschen Maygane Ronsmans Maxime Boutier Joanna Jazowiecka-Rakus and all my other colleagues…

Biostatistics (FARAH-ULg) Frédéric Farnir

Wageningen University Geert Wiegertjes Maria Forlenza Carla Piazzon de Haro

Instituto Gulbenkian de Ciencia Alekos Athanasiadis

UCL Thomas Michiels

University of Glasgow Andrew Davison

INRA Pierre Boudinot

Lancaster University Derek Gatherer