Improving food safety through the development and ...Dec 10, 2015 · Technological: Realization of...

Improving food safety through the development and

implementation of active and biodegradable food

packaging systems - ACTIBIOSAFE 1SEE/30.06.2014

Projects financed under health & food safety and

renewable energy domains

Dr. Cornelia Vasile

“P.Poni” Institute of Macromolecular Chemistry

International Conference “Achievements and future steps”

Bucharest, December 10, 2015

Project summary

Programme RO14 - “Research within Priority Sectors”

Conference “Achievements and future steps”


Promoter “Petru Poni” Institute of Macromolecular Chemistry - Iasi (PPIMC);

Principal Investigator: Prof. Dr. Cornelia VASILE

Project Partners: 1: NOFIMA AS - Team Leader: Dr. Morten SIVERTSVIK ;

2: SC Research Institute of Organic and Auxiliary Products SA (ICPAO);

Team Leader: Dr. Maria RAPA;

3: SC RODAX SRL (RODAX); Team Leader: Dr. Laurentiu MOLDOVAN;

4: University of Agronomic Sciences and Veterinary Medicine Bucharest (USAMVB);

Team Leader: Ass. Prof. Dr. Amalia MITELUT

Budget: 900 000 Eur. Duration: 2014-2017

Objective(s): Scientific: Effective knowledge sharing among the research and scientific communities: between Romania and Norway;

Technological: Realization of packaging prototypes: biodegradable film and trays, encapsulated forms for bioactive packaging

Improve the level of consumers awareness and health: Obtaining of nanostructured encapsulated systems based on

natural oils (essential and cold-pressed oils) and chitosan/ chitosan derivatives to improve the thermal stability and to

manufacture the biodegradable materials with antioxidant/antimicrobial/antifungal or/and biological activities;

Reducing food contamination risk and prolong shelf life of food products;

Environment protection: Decrease of food waste

Training: Courses elaboration, Supporting PhD theses and MSc students dissertations

Target groups/end-users of projects results: food packaging sectors; food industry, delivery/retail systems for food products.

Research groups:PPIMC – IASI

• C. Vasile• L. Profire• R. Darie-Niţă• B. Munteanu• M. Brebu• E. Stoleru• R.P. Dumitriu• A. Sdrobis• E. Părpăriță• D. Pamfil• External co-workers• O. Dragostin• G. M. Pricope, • E. G. Ioanid• T. Zaharescu• L. Oprică• F. Doroftei

4 CS I; 2 professors; 2 directors of SMEs; 2 CS II; 5 ass.prof and lecturers; 4 CS III; 2 CS; 4 AC; 6 PhD students; 5 financial representatives; 12 men; 20 women.

NOFIMA – Norway

J. T. Rosnes, M. Sivertsvik, T. Løvdal, B. T. Rotabakk

ICPAO – Medias

M. RâpăE. GrosuL. Stanulet A.TrifoiT. PapO. Blajan

RODAX - Bucharest

G. PanteaL. MoldovanM. BeceaL. Magdan

USAMV - Bucharest

A. C. MiteluțM. E. PopaM. C. DrăghiciS. Danaila-GuideaM. Geicu-CristeaE. E. Tănase,P. Popescu



Project Objectives

ACTIBIOSAFE main purpose is to prolong the shelf life of real food, (chicken and salmon fillets etc. at least 20 % increase), keeping the food quality and freshness during the time required for its commercialization and consumption, to be safe and to have no risk of affecting health.

• Effective knowledge sharing the research results among scientific communities: between Romania and Norway;

• Obtaining of nanostructured encapsulated systems based on natural oils (essential and cold-pressed oils) with chitosan and chitosan derivatives in order to improve the thermal stability and to manufacture the biodegradable materials with antioxidant/ antimicrobial/ antifungal or/and biological activities;

• Minimizing food packaging waste (millions of tons every year) and to preserve natural resources through replacing traditional plastic packaging materials with new biodegradable antimicrobial packaging biocomposites;

• Reducing food contamination risk and prolong shelf life of food products;

• Developing and implementing courses and supporting for individual PhD and MSc student projects;

• High level scientific publications



Project Objectives

• Integration of technologies:

– extrusion, thermoforming,

– activated coating by gamma irradiation/ plasma exposure,

– Electrospinning/electrospraying coating with bioactive agents

• Industrial validation of prototypes by in vivo and on food packaging equipments tests.



• WP1: Screening and characterization of raw materials

• WP2: Processes development at laboratory scale and characterization

• WP 3: Development of active food packaging

• WP 4: Quality and Safety Validation of active food packaging systems

• WP5: Dissemination, Exploitation and IPR Management

• WP6: Consortium Management

WORK PACKAGES:



Inter-multi-and trans-disciplinary key activities

• a) development of new formulations based on biodegradable polymers and chitosan/chitosan derivatives by melt-mixing processing, to meet requirements of mechanical and thermal properties and stability, cost effective and testing them from biological and toxicological point of view

• b) elaboration of methods to obtain encapsulated nanostructures. Active oils of natural substances and natural compounds (cinnamon, thyme, basil, oregano, eugenol as natural extracts, etc.) will be encapsulated into chitosan. Nanostructured materials will be obtained by optimization of electrospinning/spraying conditions.

• c) development of kinetic models for volatile compounds release to foodstuff, antioxidant and antimicrobial/antifungal efficiency, and stability.

• d) to achieve of new food packaging with antimicrobial /antioxidant /bioactive features by integration of technologies (extrusion, thermoforming, electrospinning, electrospraying, active packaging). To prove the viability of the new food packaging material, validation of the packaging prototypes will be carried out in vivo tests on real food products (shelf-life studies, physical-chemical, microbiological and sensorial evaluation) and by testing on food packaging equipments.

• e) create a consortium with producers and consumers from delivery/retail system to

implement the new materials on market.



Project outcomes (planned vs. achieved)

[…your text in max. 2 bullets:

outcomes to date …]

expected outcomes




…

Expected outcomes Outcomes to date – 3 gold medals at EUROINVENT 2015

• Selection and testing of bioactive compounds

• Chemical modification of chitosan

• Obtaining of biodegra dable,

bioactive packaging materials –composites, nanocomposites, stratified composites and encapsulated formulations with antimicrobial /antifungal /antioxidant/biological functions

• Training of young researchers and students – PhD students and Master students

• Chitosan and eight vegetable oils were tested for their composition, antimicrobial/antifungal and antioxidant activity – 1 paper accepted for publication

• Sulfadiazine- and other chitosan derivatives are obtained by original procedures – 1 ISI paper published

• Three ways are developing at laboratory/micropilot scale:

- melt processing , selection of plasticizers for PLA/PHB/PHBV – 3 ISI co-

authored papers in press- coating by a two step procedure: gamma irradiation or cold plasma pre-

treatment followed by immersion or electrospinning/coupling reaction – 1 co-authored paper sent to ISI journal – gold medal at EUROINVENT 2015

- freezing-thawing method – 1 ISI paper in press and 1 book chapter published

• 1 PhD Thesis and 2 MSc dissertations defended; international ERASMUS courses on Application of Radiation technology to food packaging materials and 3 chapters in a book ready for evaluation before sending to press

Characterization of bioactive agents

• Determination of total content of phenols and flavonoids of essential and cold press- bioactive oils

• Determination of average composition of essential oils by GC-MS

• Determination of antioxidant/radical scavenger activity of bioactive compounds by 2,2-difenil-1-picrilhidrazil (DPPH)) and acid 2,2`-azino-bis(3-etilbenzotiazolin-6-sulfonic) (ABTS), methods

• Determination of antimicrobial activity by standardized (Food safety accreditated laboratory) and new developed methods (NOFIMA)





• Essential oils:

• - Thyme F (Thymus Vulgaris) – Fares company; - Cloves F

(Caryophylli aetheroleum, dried flower buds of Syzygium

aromaticum) ; - Rosemary F (Rosmarini Aetheroleum); Ti

Tree F (Melaleuca alternifolia aetheroleum) – Fares

• Vegetable Oils

- Grape seeds; Rosehip seeds; Argan oil; Apricot oil

• Analysis techniques:

• - GC-MSD + NIST – qualitative;

- GC-FID – quantitative.

Characterization of bioactive agents

Bioactive compounds characterization



The composition of essential oils determined by GC-FID

Thyme Rosemary Ti Tree Cloves

ORAC (μTE/100g)* 15 960 330 1 078 700

α-Thujene 0.4

α-Pinene 1.6 11.4 3.9

Camphene 1.0 5.0

β-Pinene 9.4

β-Myrcene 2.5 2.5

2-Carene 2.3 10.3

p-Cymene 22.5 1.3 2.8

Limonene 2.6 3.6

Eucalyptol 43.1 2.3

γ-Terpinene 7.9 16.3

α-Terpinene 4.1

Linalol 5.6

Camphor 11.3

Borneol 1.1 3.0

4-Terpineol 38.7

α-Terpineol 1.8 4.6

Thymol 43.1

Carvacrol 2.7

Eugenol 85.7

β-Caryophyllene 2.3 3.2 2.2 4.5

Aromadendrene 1.1

Ledene 1.1

Eugenol acetate 7.9

δ-Cadinene 0.9

Caryophyllene oxide 0.4

* ORAC – oxygen radical absorption capacity; expressed as μmol

Trolox equivalent (TE) at 100g, accuracy of 5%

The content (%) of total phenolic compounds from vegetable oils

determined by Folin-Ciocalteu method

Oil Phenolic content %

Rosehip seeds 0.0123

Grape seeds 0.00522

Apricot 0.00497

Argan 0.00194

Thyme 0.02767

Clove 0.09004

Rosemary 0

Ti Tree 0.00269

•••Clo •••Thy •••Tea •••Ros0

3000

4000

5000

IC

50 [

g/m

L]

IC50 [ppm]

Rosehip seed oilArgan oil

Apricot oil

Grape seeds oil0

1000

2000

3000

4000

5000

6000

7000

8000

IC5

0 [

g/m

L]

IC50 [ppm]

The values of sample concentration required to

scavenge 50% of ABTS free radicals (IC50) of

selected essential (a) and cold-pressed oils (b)

Task 2.1 Chemical functionalization of chitosan -Chitosan Derivatives



Rd – radical in chitosan derivatives

In vitro Antimicrobial and Antioxidant Assays

DPPH and ABTS Radical

Scavenging Assay

0

10

20

30

40

50

60

70

80

3a 3b 3c 3d 3e 3f CMMW

SC

AV

EN

GIN

G

CA

PA

CIT

Y

(I%

)

COMPOUND

(1: CS, 2: OCMC,

3 – 6: chitosan

derivatives

Chitosan derivatives have better good antimicrobial / radical scavenging activities

S.a. = Staphloccocus auresus ATCC 25923, S.l.= Sarcina lutea ATCC 9341,

B.c.= Bacillus cereus ATCC 14579, B.s.= Bacillus subtilis, E.c.=

Escherichia coli ATCC 25922, P.a.= Pseudomonas aeruginosa CIP 82118.



Tasks 2.2 Preparation and optimization of PHA (PLA)/ CS formulations

Tasks 2.3. Caracterization of the developed composites



I) Melt processing: Optima formulations have been established:

Plasticized PLA properties

(a) (b)

(c)

PLA plasticization with about 15 % non-toxic selected plasticizers could yield flexible and transparent films (PLA/USE) with appropriate optical and barrier properties (PLA/PEG) for applications in the food-packaging.



Analyses of antimicrobial effects from treated packaging films

1 cover film

2 test inoculum (0,4 ml)

3 Test specimen

4 Perti dish

UNTREATED SAMPLE TREATED SAMPLE Antibacterial activity

U0 Ut At

Test no.

PHBV 3,72 1,40 0,88

Stomacher 3,61 2,94 2,42

PHB 3,80 1,97 1,17

Stomacher 3,79 2,84 2,04

1,17

2,04

1A

2A

0,52PHBV ATBC

10/CS3

PHB TBC10/CS3

PHB MB20/CS3

0,80

0,80

R=(U t - A t )

Bacteria incubated with active

film at 37 oC in 24 hours

Number of bacterial log reductions after contact with active films

Enumeration of bacteria after treatment

with antimicrobial film

Staphylococcus aureus or

Escherichia coli



Task 2.4. Design and characterization of encapsulated structures

• Three procedures have been applied for encapsulated nanostructures preparation:

• Electrospinning – particle and nanofibresencapsulated morphologies

• Mixing in presence or not of surfactants followed by solvent casting

• Phenolic extract used as powder



II) Coating by immersion or electrospinning:

Antifungal

Antibacterial

Antioxidant



The inhibition rate of the plasma treated and chitosan-grafted PLA films with the surface of 2.25

cm2 tested against Aspergillus brasiliensis ATCC 16404 fungus

Chitosan film type A1(%) A2(%) M(%)

PLA/N2 66 68 67

PLA/N2/CHT1/I 100 94 97

PLA/N2/CHT2/I 100 100 100

PLA/N2/CHT3/I 100 100 100

PLA/N2/CHT1/ES 99 91 95

PLA/N2/CHT2/ES 100 100 100

PLA/N2/CHT3/ES 100 100 100

Antibacterial activity (%) of untreated, plasma and/or irradiated PLA further modified with chitosan 1

Sample Escherichia coli Listeria

monocytogenes

Salmonella

typhimurium

PLA 52 40 55

PLA/N2/CHT1/I 100 100 96

PLA/N2/CHT2/I 100 100 97

PLA/N2/CHT3/I 100 100 93

PLA/20KGy/CHT3/I 84 96 100

2

DPPH Radical scavenging activity (RSA) of untreated, plasma and/or irradiated PLA substrate

further modified with chitosan

Sample RSA (%)

PLA 0

PLA/N2 9

PLA/20kGy 12

PLA/N2/CHT3 81

PLA/20KGy/CHT3 100



Nanostructured morphologies obtaining

PLA/plasmaPLA PLA/plasma/CHT/ES

2D

phase

Sample

Radical

scavenging activity

(RSA, %)

Bacteria growth inhibition at 48h (%)

Escherichia

coli

Listeria

monocytogenes

Salmonella

enteritidis

PLA 0 52 40 55

PLA/CHT/electrospinning (ES) 76.4 100 100 97

PLA/CHT/immersion 11.8 100 100 100

Radical scavenging activity and antimicrobial activity of untreated, plasma and

chitosan-coated PLA substrate

After 50 days incubation:

1. All the samples incubated in sterile soils

presented an increasing of weight (except

PLA/ATBC sample).

2. Only few samples started biodegradation

process and the evolution is the following:

Task 2.6. Biodegradability study of the obtained materials

After 100 days incubation:

1. The biodegradation rate was

increasing for all the samples.

2. The trend of the biodegradation rate is

similar with the first evaluation:

-1

-0,5

0

0,5

1

1,5

2

Bio

deg

rad

ati

on

ra

te (

%)

Biodegradation rate after 50 days of

incubation

Normal soil Steril soil

-2

-1,5

-1

-0,5

0

0,5

1

1,5

2

2,5

3

Bio

deg

rad

ati

on

ra

te (

%)

Biodegradation rate after 100 days of

incubation

Normal soil Steril soil



Evaluation of the

biodegradation rate of the

developed composites

The biodegradation rate of the tested samples – after 50 days

The biodegradation rate of the tested samples – after 100 days

The biodegradation trend

PLA/ATBC>

PLA/ATBC/CHc2>

PLA/ATBC/CHc1> PLA/ATBC/CHc0

The biodegradation trend

PLA/ATBC/CHc2> PLA/ATBC>

PLA/ATBC/CHc0> PLA/ATBC/CHc1



Global germination index (GI) – cucumber seeds

Task 2.7. Ecotoxicity Evaluation of novel developed materials

0

50

100

150

200

GI

(%)

Cucumber seeds

The GI is a very sensitive index

indicating the nonphytotoxicity of

the soil, when the values are

higher than 80%. In our research,

only 4 samples proved to be

nonphytotoxic over the cucumber

seeds.

Global germination index (GI) – radish seeds

0

50

100

150

200

GI

(%)

Radish seedsThe GI presented

values higher than 80%

for all tested samples,

so it demonstrates that

the tested soil is

nonphytotoxic.



Variants COMPOST SAMPLE Number of

leaves formed

SALAD

Number of leaves formed

SAGE

I.1 PLA – sterile soil 3.75 4.0

I.2 PLA 5.3 5.0

II.1 PLA/MB – sterile soil 2.0 4.0

II.2 PLA/MB 6.37 4.0

III.1 PLA/MB/CH c1 – sterile soil 5.87 2.5

III.2 PLA/MB/CH c1 7.42 6.6

IV.1 PLA/ATBC – sterile soil 6.5 2.0

IV.2 PLA/ATBC 8.22 4.0

V.1 PLA/ATBC/CH c1- sterile soil 5.8 4.6

V.2 PLA/ATBC/CH c1 7.42 2.0

VI.1 PLA/ATBC/ CH c1- sterile soil 5.62 4.0

VI.2 PLA/ATBC/ CH c1 6.0 4.0

VII.1 PLA/ATBC/ CH c2- sterile soil 5.5 2.0

VII.2 PLA/ATBC/ CH c2 7.4 4.5

0 MARTOR/CONTROL 4.88 4.0

Comparative

results obtained

from monitoring

the number of

leaves formed /

seedling

(average) to day

35 of seedlings

of lettuce and

sage.

Evaluation of ecotoxicity of the newly developed biobased products on plants



The tests have been performed using packaging equipments;

The prototypes received fromICPAO have been tested

for the following properties:Sealing propertiesIntegrity seal testsTear test / Peel testFlexural propertiesElongation

Task 2.8 Testing of sheets developed in project on the packaging equipments

Sealing testsLeaking tests

Peel tests



Dissemination summary

• Promotional materials: Website, Leaflet, Newsletter, Poster on project, Norwegian press release

• 5 papers published or in press

• 1 Book chapter in Springer Publ.

• 2 papers submitted for publication

• 3 gold medals and 1 Excellence diploma to EUROINVENT’2015

• 8 oral communications and 12 posters to international conferences



Task 2.9. Dissemination of the resultsby participation at conferences andpublication of 2 ISI papers

1. Sulfadiazine—Chitosan Conjugates and Their Polyelectrolyte Complexes with Hyaluronate Destined to the Management of Burn Wounds, R. P. Dumitriu, L. Profire, L. E. Nita, O. M. Dragostin, N. Ghetu, D. Pieptu and C. Vasile, Materials 2015, 8, doi:10.3390/ma70x000x

2. Evaluation of some eco-friendly plasticizers for PLA films processing, R.N. Darie-Nita, C. Vasile, R. Lipsa, M. Rapa, J. Appl. Polym. Sci. in press

3. Poly(vinyl alcohol)/chitosan/montmorillonite nanocomposites for food packaging applications: Influence of montmorillonite content. E. Butnaru, C. N. Cheaburu, O. Yilmaz, G. M. Pricope and C. Vasile, High Performance Polymers, DOI: 10.1177/0954008315617231.

Published Papers ISI Journals



Chapters in books

Eco-Friendly Chitosan-Based Nanocomposites:

Chemistry and Applications,

C. N. Cheaburu-Yilmaz, O. Yilmaz, and C, Vasile, Chapter in Eco-friendly

Polymer Nanocomposites. Chemistry and Applications, Eds, V. Kumar Thakur, M. K. Thakur, 341-387 , 2015, Springer, ISSN 1869-8433 ISSN 1869-8441 (electronic), Advanced Structured Materials, ISBN 978-81-322-2472-3 ISBN 978-81-322-2473-0 (eBook)

DOI 10.1007/978-81-322-2473-0, Springer New Delhi, Heidelberg, New York, Dordrecht London,http://www.springer.com,series/8611



http://www.springer.com,series/8611

Papers accepted for publication

• Effect of plasticizers on melt processability and properties of PHB, M. Râpă, R.N. Darie-Niță, E. Grosu, E.E.Tănase, A.R. Trifoi, T. Pap, C. Vasile J.Opto. Adv. Mater.

• Sustainable Alternative for Food Packaging :

Chitosan Biopolymer – a review, A. C.

Miteluţ, E. E. Tănase, V. I. Popa, M. E. Popa,

AgroLife Scientific Journal, Vol 4, No 2, 2015



Participation to international and national meetings

• 9th International Conference on Materials Science and Engineering –BRAMAT 2015, Brasov, Romania

• Oral presentation:

• II.O.10. Influence of plasticizers over some physico-chemical properties of PLA• R.N. DARIE-NIȚĂ, C. VASILE, PPIMC, Iasi, ROMANIA; M. RÂPĂ, ICPAO S.A., Medias, ROMANIA• Posters:• II.P.64. Assessment of the antifungal activity of chitosan films for new packaging materials

design• A.C. MITELUT, E.E. TANASE, A.L. MIHAI, C.P. CORNEA, M.E. POPA, M.C. DRAGHICI, University

of Agronomic Sciences and Veterinary Medicine Bucharest, ROMANIA; E. STOLERU, B.S. MUNTEANU PPICM, ROMANIA

• II.P.77. Effect of plasticizers on melt processability and properties of PHB • M. RÂPĂ, E. GROSU, A.R. TRIFOI, T. PAP,ICPAO S.A., Medias, ROMANIA; R.N. DARIE-NIȚĂ, C.

VASILE, PPIMC Iasi, ROMANIA; E.E. TĂNASE, USAMV- Bucharest, ROMANIA• II.P.92. New PLA based composites and their behaviour for food packaging applications• E.E. TĂNASE, M.E. POPA, O. POPA, USAMV, Bucharest, ROMANIA; M. RÂPĂ, S.C. I.C.P.E.

BISTRITA S.A., Bistrita, ROMANIA



COST Action MP1206 Workshop: Applications of Electrospinning in Composites, Nanofabrication, Food packaging, Pharma and Controlled Release, March, 25-27, 2015, Novi Sad,Serbia.

Oral presentations

1. Sulfadiazine release from electrospun meshes, B.S. Munteanu, R.P.

Dumitriu,E. Stoleru, L. Profire, C. Vasile – oral

2. Comparative study between electrospinning and immersion methods for

chitosan deposition onto poly(lactic acid) E. Stoleru, B.S. Munteanu, G.M.

Pricope, C. Vasile - oral.

3. Antibacterial membranes based on polyurethane/biological polymers/silver

nanoparticles, B.S. Munteanu, E. Stoleru, R.P. Dumitriu, G.M. Pricope, D.

Macocinschi, D. Filip, C. Vasile - oral.



EUROINVENT May, 14-16, 2015 Iasi

NEEFOOD – Brasov, May 2015

ORAL1, ENG. 6 Development of innovative biodegradable packaging system to improve

shelf life, quality and safety of fresh products, L. Moldovan, G. Pantea – RODAX Bucharest

2. ENG. 8 FOOD PACKAGING MATERIAL BASED ON CHITOSAN AND POLY(LACTIC ACID) E. Stoleru, B. S. Munteanu, R. N. Darie-Niță, G. M. Pricope, E. G. Ioanid, C. Vasile – PPIMC IasiA.C. Miteluț, M. E. Popa, E. E. Tănase, A. L. Mihai, M. C. Drăghici –USAMVBucharest

3. ENG. 9 CHITOSAN/NATURAL OILS AS COMPONENTS IN INNOVATIVE FORMULATIONS FOR FOOD PACKAGING, C. Vasile, B.S. Munteanu, M. Brebu, E. Stoleru, R. N. Darie-Nita – PPIMC - IasiA. C. Miteluț, M. E. Popa, E. E. Tănase, A. L. Mihai, M. C. Drăghici USAMVBJ. T. Rosnes, M. Sivertsvik, T. Løvdal, B. T. Rotabakk –NOFIMA

4. ENG. 7 POLY(VINYL ALCOHOL)/CHITOSAN NANOCOMPOSITES FOR FOOD PACKAGING APPLICATIONSE. Parparita, C- N.Yilmaz, O. Yilmaz, G-M. Pricope, C. Vasile – PPIMC



Posters• POS. 4. Assessment of the Antifungal Activity of Essential Oils for New

Food Packaging Materials Design, A. C. Mitelut, A. L. Mihai, E. E. Tănase, M. E. Popa, M. Drăghici, C. P. Cornea, M. E. Popa, M. Draghici, M. A. Brebu, C. Vasile, E. Stoleru, A. Irimia. Neefood proceedings.

• POS. 58. Antimicrobial Resistance of Staphylococcus aureus and E. coli to Essential Oils, J. T. Rosnes, L. Shinde, C. Vasile, M. A. Brebu. Neefood proceedings.

• POS. 3. Chitosan as a Biopolymer for Food Packaging Applications - A Review, A. C.Mitelut, E. E. Tănase, M. E. Popa, V. I. Popa. Neefood proceedings.

• POS. 15. Food Packaging Materials: Current Trends and Future Opportunities. E. E. Tanase, M. E. Popa, O. Popa, M. Rapa, Neefood proceedings.

The 14th International Symposium Prospects for the 3rd Millennium Agriculture, Improving food safety through the development and implementation of active and biodegradable food packaging systems. A.C. Miteluţ, M. E. Popa, E. E. Tănase, M. C. Drăghici, 24-26 September 2015, Cluj Napoca, Romania.

NEEFOOD – Brasov May 2015 continued



Papers submitted for publication

• Influence of Plasticizers Over Some Physico-chemical Properties of PLA, M. Râpă, R. N. Darie-Niță, C. Vasile, JOAM

• Novel procedure to enhance PLA surface properties by chitosan irreversible immobilization, E.Stoleru, R. P.Dumitriu, B. S. Munteanu, T. Zaharescu, E. E. Tănase, A. Mitelut, G.-L. Ailiesei, C. Vasile, Appl. Sur. Sci.



Reflections on partnership: role and contributions to the project




PP and P2: elaboration of formulations for bioactive packaging and their general characterization, elaboration of prototypes

P1: antimicrobial and migration tests

P4: antifungal test, ecotoxicity and degradability

Strong points: Strong and ethical collaboration based on: deep consideration, loyalty, partners expertise, confidence in quality of results. It started since 2008 in the framework of one FP7 project; research teams is composed by experts in their fields of interest which are interrelated. Weak points: The formulations properties should be modulated to correlate their good bioactive

activities with mechanical, permeability and migration properties What could be improved? By detailed study of relationship: composition of formulations –

properties.

P3 and P1: Upscale the research results and testing on

packaging manufacture equipments and specific characterization.

Market analysis and consumers demands evaluation

Future steps

• Future steps:- Detailed characterization of bioactive biodegradable materials obtained - Development of the nanosized encapsulated structures;- Modernization and improved functionality of electrospinning

device- Continue dissemination of results by publications, website

up-date, leaflets, newsletters, brochures, etc. sustainability of consortium: we intend to continue our collaboration

beyond this project by application to H2020, joint applications, co-authored publications, etc.

There are already proposals in MNT-ERA projects in second stage. PP is partner in two projects: IAEA and Erasmus with similar topics or related

with this, where project results are disseminated; NOFIMA also have 24

proposals (4 European ,10 regional, 8 national and 2 in other programmes)

including some aspects of this topic and training.




Thanks for inviting us at this prestigious conference,

for financial support from European Economic Area

(EEA) and Norwegian Grants, Romanian National

Authority for Scientific Research and Innovation

(ANCSI) and Romanian Ministry of European Funds

Thanks for your kind attention!




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