University of Zagreb School of Medicine

Plan of the course

Immunology

Academic year 2013/14

Course coordinators:

Professor Dora Vinji, MD, PhD Assistant Professor Vesna Lukinovi-kudar, MD, PhD

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I.I.I.I.

COURSE AIMSCOURSE AIMSCOURSE AIMSCOURSE AIMS: : : :

The course is intended to provide important and recent knowledge of cellular and molecular immunology. Students will be presented large amount of complex information within three weeks; therefore, the main aim is to teach basic concepts of experimental immunology, which are also applicable in a clinical setting. The extensive additional reading will be provided for students who would like to learn more about immune system function in health and disease. Lectures will be focused on important concepts of immune system function in physiological and pathological conditions. Seminars will include interactive discussion, as well as the presentation of clinical cases, in order to present complex mechanisms of immune response in a clear and understandable way. Practical part of the course will be performed in small groups to allow students to acquire lab-work skills related to some commonly used immunological techniques. II. COURSE STRUCTUREII. COURSE STRUCTUREII. COURSE STRUCTUREII. COURSE STRUCTURE

Lectures 7 (total 9 hours) Seminars 12 (total 26 hours) Practicals 3 (total 15 hours) Total 50 hours

WEEK 1 TYPE TOPIC (duration) Chapter Lectures Introduction to immunology (45 min)

Organization of the immune system (245 min) Innate immunity (2x45 min) Antigen processing and presentation (45 min)

Seminars Antibody structure and the generation of B cell diversity (2x45) Development of B lymphocytes (45) Humoral immunity (345 min)

Ch 4 Ch 6 Ch 9

Practicals Preparation of cell suspension, Immunoflurescence staining, Flow-cytometric analysis (6x45 min)

WEEK 2 TYPE TOPIC (duration) Chapter Lectures Regulation of the immune response (45 min)

Cytokines (45 min) Immunological tolerance (45 min)

Seminars Antigen recognition by T lymphocytes (45 min) Development of T lymphocytes (45 min) Cellular immunity (345 min) Autoimmunity (345 min)

Ch 5 (125-132) Ch 7 Ch 8 Ch 13

Practicals Application of immunological techniques in clinical medicine (345)

WEEK 3 TYPE TOPIC (duration) Chapter Lectures Seminars Immunodeficiency (245 min)

Hypersensitivity (345 min) Histocompatibility.Transplantation (345 min) Tumor immunology (245 min) Immunity to infections. Vaccination (2x45 min)

Ch 11 Ch 12 Ch 5 (145-154), Ch 15 Ch 16 Ch 10,14

Practical Blood groups. Differential. (645 min)

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III. III. III. III. PLAN OF THEPLAN OF THEPLAN OF THEPLAN OF THE COURSE AND COURSE SCHEDULECOURSE AND COURSE SCHEDULECOURSE AND COURSE SCHEDULECOURSE AND COURSE SCHEDULE

WEEK 1

Day Time Type Group Topic Faculty

Monday

7.4.14.

Tuesday

8.4.14.

9.00-9.45

10.00-11.30

12.00-13.30

L (M)

L (M)

L (M)

A+B

A+B

A+B

Introduction to immunology

Organization of the immune system

Innate immunity

Wednesday

9.4.14.

8.00-12.30

13.00-17.30

P

P

A

B

Preparation of cell suspension, immunofluor. staining, FACS

Preparation of cell suspension, immunofluor. staining, FACS

Thursday

10.4.14.

8.00-9.30

10.00-10.45

11.00-12.30

S (M)

L (M)

S (M)

B

A+B

A

Antibody structure and the generation of B cell diversity

Antigen processing and presentation

Antibody structure and the generation of B cell diversity

Friday

11.4.14.

8.00-8.45

9.00-11.15

11.30-12.15

12.30-14.45

S (M)

S (M)

S (M)

S (M)

A

A

B

B

Development of B lymphocytes

Humoral immunity

Development of B lymphocytes

Humoral immunity

WEEK 2

Day Time Type Group Topic Faculty

Monday

14.4.14.

8.00-8.45

9.00-9.45

14.00-16.15

S (M)

S (M)

P*

A

A

B

Antigen recognition by T lymphocytes

Development of T lymphocytes

Application of immunological techniques in clinical medicine

Tuesday

15.4.14.

8.00-8.45

9.00-9.45

14.00-16.15

S (M)

S (M)

P*

B

B

A

Antigen recognition by T lymphocytes

Development of T lymphocytes

Application of immunological techniques in clinical medicine

Wednesday

16.4.14.

8.00-10.15

10.30-12.45

S (M)

S (M)

A

B

Cellular immunity

Cellular immunity

Thursday

17.4.14.

8.00-8.45

9.00-9.45

10.00-10.45

L (W)

L (W)

L (W)

A+B

A+B

A+B

Regulation of the immune response

Cytokines

Immunological tolerance

Friday

18.4.14.

8.00-10.15

10.30-12.45

S (M)

S (M)

B

A

Autoimmunity

Autoimmunity

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WEEK 3

Day Time Type Group Topic Faculty

Monday

21.4.14.

EASTER

Tuesday

22.4.14.

8.00-9.30

10.00-12.15

10.45-12.15

12.45-15.00

S (NV)

S (NV)

S ()

S (NV)

A

A

B

B

Immunodeficiency

Hypersensitivity

Immunodeficiency

Hypersensitivity

Wednesday

23.4.14.

8.00-10.15

13.00-17.30

S (M)

P

B

A

Histocompatibility. Transplantation.

Blood groups. Differential.

Thursday

24.4.14.

8.00-10.15

13.00-17.30

S (M)

P

A

B

Histocompatibility. Transplantation

Blood groups. Differential.

Friday

25.4.14.

8.00-9.30

10.00-11.30

12.00-13.30

14.00-15.30

S (M)

S (M)

S (M)

S (M)

B

B

A

A

Tumor immunology

Immunity to infections. Vaccination.

Tumor immunology.

Immunity to infections. Vaccination.

L-lecture, S-seminar, P-practical, M-Masek Room, W Wickerhauser Room, NV- Nova vijecnica, - akovi

Room

The practical (P*) Application of immunological techniques in clinical medicine will be held at the Immunology

Laboratory, Rebro University Hospital, Kipatieva 12 (Prof. Drago Batini)

All other practicals will be held at the Lab, Department of Physiology.

IV. IV. IV. IV. EXAMINAEXAMINAEXAMINAEXAMINATIONTIONTIONTION Final examination (written+oral) will be organized at the end of the course (Friday, 3.5.2013., 8.00 Cackovic Room) and several times thereafter, within the academic year. Written exam (test) will include 40 multiple-choice questions. Students need to answer at least 55% of the questions correctly to pass the written exam and to qualify for taking the oral examination. Students seminar work and presentation will be evaluated and included in the final score (maximum 10% of total points). The final oral examination determines the final mark of the course.

Regular terms Date

Winter 2.5.2014.

Summer

8.7.2014.

22.7.2014.

Autumn

9.9.2014.

23.9.2014.

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V./I. LIST OF LECTURERS AND TEACHING STAFFV./I. LIST OF LECTURERS AND TEACHING STAFFV./I. LIST OF LECTURERS AND TEACHING STAFFV./I. LIST OF LECTURERS AND TEACHING STAFF

Professor Drago Batini, MD, PhD Vilma Dembitz, MD Assistant Professor Tomislav Kelava, MD, PhD Hrvoje Lali, MD Assistant Professor Vesna Lukinovi-kudar, MD, PhD Alan uur, MD Professor Dora Vinji, MD, PhD Professor Mirza iak, MD, PhD VVVVI. LITERATUREI. LITERATUREI. LITERATUREI. LITERATURE A.A.A.A. ObligatoryObligatoryObligatoryObligatory

Parham Peter. The Immune System, Third Edition, New York-London,

Garland Science, Taylor & Francis Group, 2009. B. AdditionalB. AdditionalB. AdditionalB. Additional

1. Delves PJ. Martin SJ, Burton DR, Roitt IM, Roitt's Essential Immunology, 12th edition. Wiley-Blackwell, 2011.

2. Abbas AK, Lichtman H, Pallai S. Cellular and molecular immunology, 7th edition, Philadelphia Saunders, 2011.

3. Murphy K. Janeway's Immunobiology, 8th edition. New York: Garland Publishing; 2011. 4. Geha RS, Rosen FS. Case studies in immunology: A clinical companion, 5th edition. New York: Garland

Publishing; 2007. 5. Johnson AG, Ziegler RJ, Hawley LB. Microbiology & Immunology: Board Review Series, 5th edition.

Philadelphia: Lippincott Williams & Wilkins; 2009.