IDENTIFICATION OF NEUROSECRETORY MOLECULES IN APLYSIA CALIFORNICA

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IDENTIFICATION OF NEUROSECRETORY MOLECULES IN APLYSIA CALIFORNICA AND RELATED MOLLUSCS: GENOMIC APPROACHES

By

JINNIE AMBER SLOAN

A THESIS PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT

OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE

UNIVERSITY OF FLORIDA

2009

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© 2009 Jinnie Amber Sloan

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To my mother, Lisa, and my family for all their suppor t and love

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ACKNOWLEDGMENTS

I would like to thank my colleagues, collaborators and teachers at the Whitney Laboratory

for Marine Biosciences. This work is a reflection of the keen insight and vision of my mentor,

Leonid L. Moroz, who always strives to push science and those around him to accomplish what

may seem impossible. I am grateful to my dissertation committee, Peter Anderson and Nancy

Denslow. I will be forever grateful to Andrea Kohn for her continual support and guidance in

my pursuits bo th as a scientist and as a friend. To Mathew Citarella, I would like to thank for

years of suppo rt and his contribution to the bioinformatics aspects of this work, none of this

would have been possible without his help. Jim Netherton, Yelena Bobkova, and Rebecca Virata

for their technical support. I am also grateful to Thomas Ha for teaching me in situ hybridization

protocols and the semi- intact preparation.

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TABLE OF CONTENTS page

ACKNOWLEDGMENTS ...............................................................................................................4

LIST OF TABLES ...........................................................................................................................8

LIST OF FIGURES .........................................................................................................................9

GLOSSARY OF TERMS ..............................................................................................................12

ABSTRACT ..................................................................................................................................13

CHAPTER

1 INTRODUCTION ..................................................................................................................16

Introduction .............................................................................................................................16Aplysia californica: a model for learning and memory ..........................................................16Neuropeptides .........................................................................................................................17

2 IDENTIFICATION OF NEUROSECRETORY PRODUCTS IN GASTROPOD MOLLUSCS ...........................................................................................................................23

Introduction .............................................................................................................................23Results and Discussion ...........................................................................................................25

Identification of Predicted Secreted Signal Molecules ...................................................25Homology results ......................................................................................................25Prediction results ......................................................................................................25

Confirmation of Expression of Selected Neuronal Transcripts .......................................28Cross-Species Analysis ...................................................................................................29Genomic Organization of Selected Neurosecretory Products .........................................29

Conclusions .............................................................................................................................30Methods and Materials ...........................................................................................................31

Animals and Tissue Collections ......................................................................................31454 L ibrary Construction ................................................................................................32

3' end amplified cDNA library construction for 454 sequencing: ............................325' end target amplified cDNA library construction for 454 sequencing: .................33

Cloning of selected Signaling Molecules ........................................................................34Hypothetical protein 2 ..............................................................................................34LFRFamide precursor ...............................................................................................35Myomodulin- like Neuropeptide Precursor 3 ............................................................35Conopressin ..............................................................................................................35Ependymin-related protein 2 ....................................................................................35Betsin ........................................................................................................................35Feeding circuit activating petide precursor 3 (FCAP-3) ..........................................36SN4 ...........................................................................................................................36

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SFY1-like peptide .....................................................................................................36SFY3-like peptide .....................................................................................................36FIRFamide related neuropeptide precursor ..............................................................36Major royal jelly protein (MRJ) ...............................................................................36FMRFamide 5 ...........................................................................................................37Allatotropin-OR precursor ........................................................................................37Second ......................................................................................................................37

Neurosecretory Predictions .............................................................................................37Homology search us ing unbiased shotgun approach ................................................37Genomic approach ....................................................................................................38Classical Secretory Prediction* ................................................................................38Non-classical Secretory peptide Prediction* ............................................................39Cross-Species Analysis of Predicted Proteins ..........................................................39Annotation of Predicted Proteins ..............................................................................40

3 MAPPING EXPRESSION OF NEUROPEPTIDE TRANSCRIPTS.....................................50

Introduction .............................................................................................................................50Mytilus Inhibitory Peptides ..............................................................................................50Fulicin ..............................................................................................................................51

Results .....................................................................................................................................51Cloning of Aplysia Fulicin precursor mRNA ..................................................................51Localization of Fulicin in the CNS of Aplysia ................................................................52Co-localization of MIP and Fulicin .................................................................................52Isolation of Fulicin in the CNS of Aplysia ......................................................................53MIP and Fulicin share 5’ UTR and coding region ..........................................................53TxFrag2: Structure and F unction .....................................................................................53

Discussion ...............................................................................................................................54Colocalization of Fulicin and MIP ..................................................................................54TxFrag2: Modulator of Expression? ...............................................................................55

Materials and Methods ...........................................................................................................56Animals ............................................................................................................................56Cloning of full- length cDNA encoding Fulicin ...............................................................56Sequence ana lysis and Alignments .................................................................................56In-situ hybridization of Fulicin and MIP in Aplysia .......................................................57Imaging ............................................................................................................................58

4 GENE EXPRESSION PROFILING FOR INDIVIDUAL NEURONS .................................65

Introduction .............................................................................................................................65Advantages of Aplysia .....................................................................................................65Limitations of Sequencing Technology ...........................................................................66

Results and Discussion ...........................................................................................................67Sensory Neurons ..............................................................................................................67Motor Neurons .................................................................................................................67Interneurons .....................................................................................................................68

Discussion ...............................................................................................................................69

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Digital Expression Profling .............................................................................................69

APPENDIX

A MODERN VIEW OF ANIMAL PHYLOGENY .................................................................220

B MOLLUSCAN CLASSES ...................................................................................................222

LIST OF REFERENCES .............................................................................................................223

BIOGRAPHICAL SKETCH .......................................................................................................229

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LIST OF TABLES

Table

page

2-1 Annotation of Lottia predicted secretory products against NCBI’s NR database. ............45

2-2 Summary of Cross Species Predictions. ............................................................................46

2-3 Genomic Organization of Neurosecretory Genes. ............................................................48

2-4 Adaptors and Primers for 5’ and 3’ 454 libraries ..............................................................49

3-1 Comparison of Real Time PCR of Aplysia MIP-related gene and 454 sequencing. .............

4-1 Validation of DEP using in situ hybridization ...................................................................76

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LIST OF FIGURES

Figure

page

1-1 The Aplysia model system.. ...............................................................................................20

1-3 Conserved motifs found in neuropeptides. ........................................................................22

2-1 Summary of model organism and number of ESTs/cDNAs collected. .............................41

2-3 Conserved Signaling Molecule Motif. ...............................................................................43

2-4 Workflow for Computational Prediction of SSMs. ...........................................................44

2-5 Comparative analysis of predicted Lottia secretory products. ...........................................47

3-1 Fulicin Gene and Protein Predictions ................................................................................59

3-2 Schematic overview of the distribution of Fulicin- like transcript in the CNS of Aplysia. ...............................................................................................................................60

3-3 Colocalized expression of MIP and Fulicin transcripts. ....................................................61

3-4 Alignment of the coding region of Aplysia MIP-like protein against the identified Aplysia Fulicin- like protein. ...............................................................................................63

4-1 Semi- intact preparation of Aplysia abdominal for identifying neurons of the gill and siphon withdraw reflex. ......................................................................................................72

4-2 Digital expression of neurosecretory products predicted by traditional cloning. ..............73

4-4 Digital expression of neurosecretory products predicted by genomic approach. ..............75

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LIST OF OBJECTS

Object

page

2-1 Intron/Exon Boundaries of selected neurosecretory genes. ...............................................77

2-2 Identified neurosecretory products likely to be signaling molecules. ................................82

2-3 Controversial predicted neurosecretory products. ...........................................................127

2-4 Predicted Lottia secreted signa ling proteins. ...................................................................149

2-5 Controversial predicted Lottia secreted signaling proteins. .............................................155

2-6 Predicted signaling molecules found in Lymnaea stagnalis. ...........................................170

2-7 Predicted secreted signaling molecules found in Pleurobranchaea californica. .............181

2-8 Predicted secreted signaling molecules found in Tritonia diomedea. .............................189

2-9 Predicted secreted signaling molecules found in Melibe leonine ....................................195

2-10 Predicted secreted signaling molecules found in Clione limacine. ..................................199

2-11 Predicted secreted signaling molecules found in Octopus vulgaris. ................................201

2-12 Predicted secreted signaling molecules found in Nautilus pompilius. ............................209

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LIST OF ABBREVIATIONS

BLAST Basic Local Alignment Search Tool

CNS Central Nervous System

ER Endop lasmic reticulum

ESTs Expressed Sequence Tags

DEP Digital Expression Profile

IN Interneuron

MN Motor Neuron

NCBI National Center for Biotechnology Information

ncRNA Noncoding RNA

NR protein database for Blast searches, compiled by NCBI

PCR Polymerase Chain Reaction

RACE Rapid Amplification of cDNA Ends

SN Sensory Neuron

sRNA Short Noncoding RNA

SSMs Secreted Signaling Molecules

UTR Untranslated region

Symbols

* Cloned by Jinnie Sloan

+ Cloned by the Moroz lab

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GLOSSARY OF TERMS

• BLAST: finds regions of local similarity between sequences. This program can be used to compare nucleotide or protein sequences to a sequence database in order to determine the functional and evolut ionary relationship be tween sequences. The statistical signi ficance calculated between sequences can be used as a guide to compare sequences and identify members of gene families.

• Exon: the nucleic acid sequene within a gene that is present in the mature form of an RNA molecule.

• In situ hybridization: used to localize a specific DNA or RNA sequence in a specific

tissue or cells by using labeled complementary DNA or RNA. Transfrag • Intron: a region of DNA within a gene that is not translated into protein. These regions

are included in the transcribed pre-mRNA and removed by splicing to produe a mature RNA.

• Neuropeptide : small protein- like molecules that can be secreted by neurons to

communicate with each other. Neuropeptides can act on neighboring neurons as anterograde or retrograde messengers or the neuron secreting the neuropeptide (orthograde messengers) through cell surface receptors to modulate or mediate neuronal communication. Typically neuropeptides alter the communication of a neuron by increasing or decreasing its excitability. Neuropeptides are assembled by ribosomes attached to the ER then transferred to the Golgi Apparatus to be packaged into vesicles and transported to synaptic terminals. Some neuropeptides are secreted directly into the blood stream and are referred to as neurohormones. Secreted neuropeptides can have long lasting effects from seconds to days.

• Prepropeptide: the inactive precursor of a peptide that requires posttranslational

modifications to become the active peptide molecule. The prepropeptide of neuropeptides is characterized by a signal peptide that targets the protein to the secretory pathway for posttranslational modifications including the cleavage of the signa l peptide in the ER.

• Signal Peptide : a shor t (3-60 amino acid) sequence made up of a positive ly charged

sequence found on the N-terminal region, a hydrophobic region and a polar uncharged C-terminal region (cleavage site) that targets the transpo rtation of a protein. In the case of secretory signaling peptides, the signal peptide targets the preprotein to the ER for further posttranslational modifications.

• Transcript: an RNA molecule produced from a gene. • Transmembrane Domain: a short span of amino acids (15-35) composed of mostly

hydrophobic regions separated by polar connecting loops that form stable secondary structures in membranes.

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ABSTRACT OF THESIS PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE

REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE

IDENTIFICATION OF NEUROSECRETORY MOLECULES IN APLYSIA CALIFORNICA

AND RELATED MOLLUSCS: GENOMIC APPROACHES

By

Jinnie Amber Sloan

August 2009 Chair: Leonid L. Moroz Major: Medical Sciences

Several major questions related to the molecular underpinnings of neuronal identity and

function such as, “What makes a neuron a neuron? What is the genomic basis of unique neuronal

phenotypes? How different is the transcriptiona l profile of one neuron from another? Can

molecular techniques be used to determine neuronal identity?” remain at the center of research in

neuroscience. As a first step to answering these questions, and providing a list of molecular

markers to identify specific neurons types, we have attempted to identify and quantify nearly all

transcripts likely to be uniquely expressed in different neuronal classes (such as neuron-specific

secretory products) and p lay a crucial role in neuron identity and func tion. This inc ludes

transcripts that encode neuropeptides, prohormones and related secretory signal peptides.

Molluscs have served as powerful model organisms for cellular and system neuroscience

for more than 40 years (McPhie and Miller, M., 2006; Kandel, 1970). Their nervous systems

consist of simplified networks of large identified neurons, allowing unprecedented opportunities

to study the pr inc iples of organization of neural circuits as well as learning and memory

mechanisms. As our major experimental models, we chose Aplysia californica and related

species, sea slugs belonging to the class of Gastropod Mollusca and species belonging to

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cephalopod molluscs. Our long-term goal is to identify neurosecretory molecules of peptide

nature using a combination of comparative and genomic approaches. We have chosen

neurosecretory molecules as putative molecular markers because they are highly abundant in

neurons and are responsible for cell signa ling and modulation. Originally, the major limitation in

using Aplysia californica, as well as other molluscs, has been the lack of genomic information

available for these model species.

To overcome this limitation, we have aimed to br idge the gap be tween genomic and non-

genomic models. The Moroz lab has sequenced >980,000 ESTs/cDNAs from eight key

mollusan species (Gastropods: Aplysia californica, Pleurobranchaea californica, Clione

limacina, Tritonia diomedea, Melibe leonina, Lymnaea stagnalis, and Cephalopods: Octopus

vulgaris, Nautilus pompilius). These sequences were assembled and cross-annotated using the

extensive transcriptome and genomic information from Aplysia californica. My thesis deals with

comparative analysis and identification of bo th evolut ionary conserved and novel transcripts that

encode neuropeptides, prohormones and other predicted secretory products. As a part of the

project, we have also employed computational approaches to predict novel signal molecules

based on shared predicted protein motifs that are conserved across all secreted signa ling proteins.

Through this work, I identified a selected list of candidate transcripts predicted to encode

secretory molecules across selected molluscs and identified putative neuropeptides present in

individual neuronal classes inc luding motor neurons, sensory neurons, and interneurons. This

work also led to the identification of a set of neuropeptides that is co-expressed in sensory and

motor neurons. The developed molecular resources and the ability to map gene expression has

allowed me to provide a detailed study of the genomics of identified cells and provide a critical

bridge between genes, circuits and behavior in the broad evolut ionary context. Overall these

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identified neurosecretory products may be the first step to creating a comprehensive list of gene

products expressed in individual neurons that can be used for molecular identification of neuron

types.

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CHAPTER 1 INTRODUCTION

Introduction

One major goa l of neuroscience is to understand the molecular mechanisms underlying

learning and memory. Despite several advances in our understanding of these mechanisms, the

complexity of the mammalian brain poses many technical challenges to studying mechanisms of

synaptic plasticity and learning. The small neuron size of mammalian brains makes isolation of

individual neurons difficult and the overall complexity of neuronal networks affecting behavior

poses problems for correlating neuronal function to specific behaviors. For this reason, many

researchers turn to simpler model organisms, such as invertebrates, for studying learning and

memor y.

Aplysia californica: a model for learning and memory

Aplysia californica (Figure 1-1) is an important model for studying mechanisms of

learning and memory because it has a simple nervous system that consists of 20,000 relatively

large and identifiable neurons (Figure 1-1) (Kandel, 1979). The nervous system of Aplysia

consists of a system of ten connected ganglia with specialized functions (Figure 1-2). The

simple and quantifiable behavior, the gill and siphon withdraw reflex, has been extensive ly

studied over the last 40 years providing keys to the cellular understanding of learning and

memor y (Kandel, 1976; Kandel, 2001; McPhie, 2006). Although about 200 neurons identified in

the central ganglia can participate in the gill and siphon withdraw reflex, the cellular circuitry

that modifies this reflex can be simplified to a network of monosynaptic connections between

three neuron types: a presynaptic sensory neuron, a post synaptic motor neuron and a modulatory

interneuron ((Kandel, 1976; Kandel, 2001). When the application of serotonin (5-HT) or nitric

oxide (NO) are applied locally to substitute for the action of the facilitory interneuron (Antonov

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et al., 2007), this experimental set up can be further simplified in cell culture to consist of two

neurons, a sensory cell (i.e. SN) and a motor neuron (i.e. L7) and still exhibit many of the same

behavioral and cellular responses seen in mammalian conditioning including long-term synaptic

plasticity and memory (Figure 1-1) (Kandel, 2001; Lewin and Walters, 1999).

The synaptic strengt h or ability for these neurons to communicate can be changed, a term

called synaptic plasticity. Modification to neuronal circuits through synaptic plasticity is

responsible for different types of learning. One major obstacle in understanding how changes in

synaptic plasticity affects learning and behavior is the heterogeneity of neuron types. Since each

neuron may be unique, it is likely that they form and maintain information in different ways.

Before the mechanism of plastcity can be understood, neuron types (motor vs. sensory vs. inter

neurons) need to reliably identified by markers. In mammals, there is strong evidence that

neurotransmitters are in part responsible for changes in synaptic strength. Here, I propose the

use of neurotransmitters, neuropeptides in particular, as neuronal markers to aid research of

neurona l plasticity.

Neuropeptides

Neuropeptides are small peptides used by neurons to communicate to one another. They

are the most diverse group of neuronal secreted chemical messengers and they function as

hormones, neuromodulators or neurotransmitters to regulate physiological processes. Cellular

signaling through neuropeptides allows neurons to modulate the central and peripheral nervous

system in both vertebrates and invertebrates (Strand et al., 1991). Neuropeptides can also

regulate intercellular signa ling (Caneparo et al., 2007), disease-host response (Boldajipour et al.,

2008; Merritt, 2007), embryonic development (Ugriumov, 2009) and or ganogenesis (Pickart et

al., 2006). Despite a range of functional roles, all classical neuropeptides are targeted to and

processed by the regulated secretory pathway via conserved protein motifs (Figure 1-3).

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Before they are posttranslationally processed into small peptides by enzymatic cleavage,

neuropeptides exist as a larger prepropeptide (Southey et al., 2008). This prepropeptide is

targeted for cotranslational translocation (CTT) into the endop lasmic reticulum (ER) by an N-

terminal signal sequence, or signal peptide. Signal peptides are composed of three regions: a

positively charged N-terminal region, a hydrophobic region, and a polar uncharged C-terminal

region. Only the C-terminal region typically shows conserved properties due to the presence of

the cleavage site (Emanuelsson et al., 2007). Only those proteins lacking a transmembrane

domain will pass through the ER and eventually be secreted outside of the outer cell membrane.

Secondary signal sequences on neuropeptides then interact with chaperone proteins to target the

neuropeptide to secretory vesicles. Not all proteins that pass through the ER are targeted to

vesicles that fuse with the plasma membrane to release its contents outside of the cell; those with

different secondary signa l sequences will be directed to various terminal destinations including

the cell membrane, the ER, the Golgi apparatus, and other organelles (Klee, 2008).

Prepropeptides are then enzymatically cleaved to release functional neuropeptides. Some

prepropeptides are made of highly repetitive units, and are processed to release several similar if

not identical peptides. Other prepropeptides are made of unique units.

Research in mollusks has revealed expression of numerous families of neuropeptides

(Krajniak et al., 1989; Smit et al., 1991), as well as individual neuropeptides (Banvolgyi et al.,

2000; Kuroki et al., 1990; Smit et al., 1992). Physiological studies in Aplysia californica have

demonstrated that neuropeptide modulation plays a crucial role in the initiation and regulation of

several behaviors including feeding, egg laying, and cardio activity (Campanelli and Scheller,

1987; Scheller et al., 1983; Sossin et al., 1987; Sweedler et al., 2002). Due to the lack of

genomic information on Aplysia, it is likely that most neuropeptides remain unidentified. Using

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the conserved structural architecture of neuropeptides, we will screen our large collection of

neuronal transcripts from Aplysia and identify putative neuropeptides.

Using the large neurons of Aplysia and unbiased shotgun sequencing, we can approach full

coverage of all transcripts expressed in individual neurons in a simple memory forming network.

It is my hypot hesis that by us ing the variability of peptide, protein, and related secretory product

expression in different neuron types, that I will be able to identify patterns of peptide and protein

expression that would give a signature for individual cell types. Coupled with the upcoming

release of the Aplysia genome, this model system provides a unique opportunity to complete

genome-wide annotation of individual neurons to identifiy molecular markers of neuronal

identity.

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Figure 1-1. The Aplysia model system.. A) Image of Aplysia californica. (Image courtesy of (Rudman, 2003). B) The abdominal ganglia from Aplysia, showing large, easily identifiable neurons. C) The gill and siphon withdraw reflex can be simplified in cell culture to two neurons, a sensory cell (i.e. SN) and a motor neuron (i.e. L7) and still exhibit many of the same behavioral and cellular responses seen in mammalian conditioning including long-term synaptic plasticity and memory. [ Image A courtesy of Rudman, W.B. (2003). Ink glands (Syndney, Sea Slug Forum). Images B and C courtesy of Lovell and Moroz, unpublished.]

1mm 1mm

A B

SN

L7 1

+

Serotonin (5 - HT)

0.2mm

Motor neurons Sensory

neurons

Motor neurons Sensory

neurons

C

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Figure 1-2. Organization of the Aplysia central nervous system. A) Diagram showing the central ganglia that make up the central nervous system of Aplysia. B) Representations of Aplysia showing the areas, color coded as in (A), innervated by specific ganglia. Some ganglia (pleural/pedal) have multiple functions innervating the same regions of the bod y as ind icataed by the gradient coloring, however there is some specificity in innervating. [Modified from Moroz, L.L., Edwards, J.R., Puthanveettil, S.V., Kohn, A.B., Ha, T., Heyland, A., Knudsen, B., Sahni, A., Yu, F., Liu, L., et al. (2006). Neuronal transcriptome of Aplysia: neuronal compartments and circuitry. Cell 127, 1453-1467.]

Head ganglia

Buccal g.

Pleural g.Pedal g.

Cerebral g.

Abdominal g

Buccal mass

Intestine

Buccal massBuccal mass

IntestineIntestine

Eye

Parapodium

Foot

Parapodium

Foot

Mantle and Visceral HumpMantle and Visceral Hump

A. B.

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Figure 1-3. Conserved motifs found in neuropeptides. All classical neuropeptides are targeted to the secretory pathway by signal peptides, short positively charged N-terminal regions found on the C-terminal region of a prepropeptide (Klee, 2008; Schatz and Dobberstein, 1996). The larger prepropeptides that encode neuropeptides are also characterized by regions of intrinsic disorder, and internal repeats that usually indicate the regions where peptides will be processed from. Importantly, prepropeptides destined to be fully processed into small peptides lack transmembrane domains (Corsi and Schekman, 1996).

Signal Peptide

Intrinsic Disorder Internal Repeats

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CHAPTER 2 IDENTIFICATION OF NEUROSECRETORY PRODUCTS IN GASTROPOD MOLLUSCS

Introduction

Gastropod molluscs have served as powerful model organisms for cellular and system

neuroscience for more than 60 years. The central nervous system (CNS) of these models

consists of a simplified network of relatively large identified neurons, allowing unprecedented

opportunities to study the principles of organization of neural circuits as well as learning and

memory mechanisms. However, a major limitation to identifiying the neurosecretory products of

neurons of the molluscan models has been the lack of genomic information.

To overcome these limitations, we have sequenced >980,000 ESTs/cDNAs from the CNS

of eight molluscan species (Gastropods: Aplysia californica, Pleurobranchaea californica,

Clione limacina, Tritonia diomedea, Melibe leonina, Lymnaea stagnalis, and Cephalopods:

Octopus vulgaris, Nautilus pompilius) (Figure 2-1). These sequences were assembled and cross-

annotated using the extensive transcriptome and genomic information from Aplysia californica

(Moroz et al., 2006). This comparative approach allowed identification of both evolutionary

conserved neuronal genes and numerous novel genes includ ing neuropeptides, prohormones and

other predicted secretory products. It is estimated that there are >320,000 putative unique gene

products (including non-coding and small RNAs) present in our comparative neurogenomic

database, which likely correspond to >50-60% of the total number of genes expressed in the

nervous systems of these molluscs.

A selected list of genes identified in that study represents major group of transcripts

implicated in the control of neural excitability, synaptic functions and plasticity, receptors,

adhesion molecules, developmental genes, and homologs of genes involved in neurological

disorders, etc. Specifically, we looked for putative neurosecretory products that may function as

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signaling molecules since these are important for modulation of the central and peripheral

nervous system in bot h vertebrates and invertebrates are involved in neuron communication,

identity and development. Most neurosecretory signaling molecules appear to be neuron-

specific, making them ideal candidates for markers for neuronal identification and to understand

the genomic basis for neuronal identity.

Previous work using released genomes to screen for secreted signaling molecules and

neuropeptides precursors. Several models including Danio rerio (Klee, 2008), Drosophila

(Wegener and Gorbashov, 2008), Arabidopsis thaliana (Emanuelsson et al., 2000), Apis

mellifera (Hummon et al., 2006), Tribolium castaneum (Amare and Sweedler, 2007) and Homo

sapiens (Emanuelsson et al., 2000) have been investigated. These results have been useful for

understanding organism-wide expression of SSMs; however, none of these predictions have

aimed at understanding neuron-specific expression of SSMs due to limitations in cell-specific

mapping in these models. The large and easily identifiable neurons of Aplysia will allow the

current work to go beyond the scope of these investigations to look at what SSMs are responsible

for learning and memory, neuron identity and plasticity in single neurons.

At the start of this project, 31 non redundant signaling peptides had been found by

traditional cloning techniques and submitted to NCBI for Aplysia californica (Object 2-2). This

represents over 20 years of work by numerous labs searching for individual proteins. Here, a

comprehensive list was created using both genomic and transcriptomic screening to identify all

non redundant transcripts predicted to encode secretory signaling peptides expressed in the CNS

of Aplysia californica. It is shown that screening genomic and transcriptomic data for transcripts

encoding proteins of interest represents a faster, more comprehensive way to identify putative

SSMs important for neuronal processes.

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Results and Discussion

Identification of Predicted Secreted Signal Molecules

Homology results

Through cross species homology search, 109 putative neuropeptides were identified from

the Aplysia CNS transcriptome, 88 predicted to be secreted signaling molecules (Object 2-2),

and 21 predicted to be involved in the secretory pathway though not necessarily secreted (Object

2-3).

While homology searches can provide a wealth of information when initially annotating

transcriptomes, it limits annotation to only sequences with homology to known sequences.

Indeed when annotating the >203,000 transcripts of the Aplysia transcriptome (Moroz et al,

2006), only 43,672 sequences can be annotated, showing the enormous complexity of the

neuronal transcriptome (Figure 2-2, insert). Furthermore, preliminary results from cross-

species transcriptome analysis suggest that many neuropeptides are quickly evolving even

between closely related species (Figure 2-2). Due to these limitations, we suspected that many

SSMs of the Aplysia CNS could not be identified through homology searches, and decided to

employ bioinformatics approaches for a more comprehensive list of SSMs using genomic scale

searches.

Prediction results

To identify novel and unannotated SSMs, publicly available software was used to complete

genome-scale profiling of all SSMs. These software programs rely upon conserved protein

motifs to predict prepropeptides. Signal peptide prediction software identifies conserved protein

motifs required for processing and directing proteins to secretory pathways (Figure 2-3).

Accurate predictions rely upon the use of full length peptide sequences. Because there is

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currently no available genome for Aplysia and the Aplysia transcriptome represents a collection

of pa rtial length sequences, a set of full length protein models with which we could perform a

computational secretome prediction is lacking. Therefore, proteins predicted from the genome of

Lottia gigantea, a related marine mollusc, were used for computational SSM prediction. Lottia

is a limpet belonging to a basal branch of Gastropoda. It was chosen as the first mollusc to have

its genome completed due to its small predicted genome. The availability of the Lottia genome

made it possible to predict putative secreted signa ling molecules that could then be used to

search the Aplysia transcriptome for secretory products and effectively bridge the gap between

genomic and non-genomic species.

A computational flowchart was used to screen the Lottia genome for SSMs (Figure 2-4).

First, a set of 23,851 full length proteins predicted by the Lottia Filtered Gene Models was

downloaded from the JGI website. The Filtered Gene Models includes gene models selected as

the best representative model available for each gene via a second layer of bioinformatics

methods including manual annotation and the use of experimental data. Protein sequences were

then screened for the presence of a signal peptide. Signal peptides are predicted by a

characteristic N-terminal sequence 15-40 amino acids that contains 2-5 positively charged amino

acids, 7-15 hydrophobic amino acids, and 3-7 neutral (often polar) amino acids that make up the

cleavage site. Through the use of TargetP 1.1 (Emanuelsson et al., 2000), 3640 were predicted

by the presence of a signal peptide to be directed to the secretory pathway. 1853 of those proteins

were further predicted to contain a signal sequence by SignalP 3.0 (Bendtsen et al., 2004b). Not

all proteins with signal peptides are secreted, like receptor proteins. To remove possible non-

secreted proteins, proteins were screened for the presence of transmembrane domains by looking

for repeated hydrophobic amino acid sequences 15-35 amino acids in length separated by polar

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connecting loops. Following screening for transmembrane domains by TMHMM 2.0c, 1034

candidate proteins were predicted to have no transmembrane domains. 859 proteins were

confirmed to have a signal sequence and no transmembrane domains by Phob ius (Kall et al.,

2004) (see materials and methods for further information about prediction software).

All sequences were then manually screened through SMART for transmembrane domains,

and protein domains to remove any possible receptors or enzymes from the list. To ensure that

predicted SSMs were likely to represent SSMs actually expressed by molluscs, only those SSMs

shown to be expressed in the transcriptomes of two or more of our molluscan species were kept

on the list. 87 predicted SSMs were predicted to be classically secreted proteins in Lottia,

including 22 putative neurosecretory products likely to be signaling molecules (Object 2-4) and

65 of controversial or unknown function (Object 2-5).

Interestingly, more proteins (937) were identified by SecretomeP (Bendtsen et al., 2004a;

Bendtsen et al., 2004b) as secreted via a non-classical secretory pathway compared to those

predicted to be secreted via classical pathways. Nonclassically secreted proteins are those

proteins lacking a signal peptide that are still secreted by the cell. These inc lude some growth

factors, interleukins and galectins. One possible explanation for this difference is that the

SecretomeP program has inherent problems for prediction with our model species. SecretomeP

relies upon machine learning algorithms to predict secretion from sequence information and

currently has only been trained agains t Gram-positive bacterial, Gram-negative bacterial and

mammalian sequences. The lack of training for invertebrate proteins may result in false

positives. In fact, closer inspection revealed that many of the secretory products predicted by

SecretomeP were annotated as molecules that rarely leave the cell.

28

Annotation of the Lottia SSMs against NCBI’s NR database reveal that 14 c lassically-

secreted peptides had no hits to the database and therefore could not be annotated (Table 2-1,

Object 2-5). This could be a product of the evolutionary distance between Lottia and other

commonly sequenced organisms used in the NR database. If the species represented by the

sequences in NR are divergent enough from Lottia, the predicted proteins will not be similar

enough to the database entries to provide significant hits for annotation. This would suggest that

homology search alone is insufficient for cataloging a list of molecular markers for neurons in

the Aplysia CNS. The fact that more than 24% of the classically-secreted peptides were

overlooked by homology searching suggests that the use of bioinformatic approaches is crucial

to identify all putative SSMs.

Alignment of the 87 p redicted Lottia SSMs against the Aplysia CNS transcriptome reveal

73 homologous putative neurosecretory molecules in Aplysia; 21 are predicted to be secreted

signaling molecules (Object 2-2), 28 are likely to be involved in the secretory pathway though

not necessarily signaling molecules, and 24 are of unknown function (Object 2-3).

Confirmation of Expression of Selected Neuronal Transcripts

As expected, the Aplysia neuronal transcriptome expresses many secreted signaling

molecules including classical neuropeptides, growth factors, and hormones. To ensure that

SSMs found through transcriptome analysis are expressed, and to determine the full length

sequence of each SSM, 94 of the identified 194 SSMs have been cloned from Aplysia cDNAs

(including those previously submitted to NCBI by other labs). For this work I have cloned 15

newly identified SSMs including six full length gene products and nine partial sequences

(sequence information can be found in Object 2-2).

29

Cross-Species Analysis

Using the analysis of predicted Lottia SSMs, a cross-species transcriptome analysis of

Pleurobranchaea californica, Clione limacina, Tritonia diomedea, Melibe leonina, Lymnaea

stagnalis, Octopus vulgaris, and Nautilus pompilius revealed homologous predicted SSMs in all

related molluscs (Table 2-2). For each species, the percentage of all Lottia secretory proteins for

which homologs exist varies across species (Figure 2-5), suggesting different evolutionary rates

between species. Species with more homologous proteins may be closer common ancestors to

Lottia than those with few homologs. O ne precaution to such conclus ions however, is that the

number of transcripts in the EST collection for each species varies (i.e. 273,922 in Lymnaea vs.

10,209 in Melibe) and fewer homologs may be present simply as a function of low coverage.

My analysis also shows that classically and non-classically secreted proteins evolve at different

rates across species (Figure 2-5). There is a consistently higher percentage of non-classically

secreted proteins than classically secreted proteins found in each species, suggesting non-

classically secreted proteins are more highly conserved.

Genomic Organization of Selected Neurosecretory Products

Our preliminary comparison of predicted neurosecretory products across species revealed

they are likely subject to great evolutionary divergence. To de termine if variability in

evolut ionary divergence may be due to differences in the genomic organization, the intron/exon

boundaries of selected neurosecretory products were analyzed using the recently released Aplysia

genome, available on trace archives of NCBI. Most vertebrate genes are composed of seven to

eight exons, however the number of intron/exon boundaries found in the selected neuropeptides

from Aplysia is relatively small, an average of four exons (Table 2-3). While there is some

variation in the number of exons between selected neuropeptides (most have 3, 4 or 5 exons),

30

there does not appear to be any clear correlation between the number of exons and the

conservation or divergence of the neuropeptide. (Object 2-1).

Conclusions

This comparative approach allowed identification of both evolutionary conserved neuronal

genes products and numerous novel predicted secreted proteins including neuropeptides,

prohormones and other predicted secretory products. Our results reveal that homologous

searching of transcriptomes is the quickest way to identify functionally relevant transcripts for

neuronal processes. However, genomic approaches are more useful for identifying novel

transcripts, though the computational resources and manual screening require significantly

greater investment in time.

Part of the obstacle to using genomic approaches is identifying functionally relevant

transcripts that are likely to be expressed at the protein level, and not merely represent

untranscribed regions of the genome. To ensure that the predicted transcripts are likely to be

transcribed, the sequences were screened against eight gastropod CNS transcriptomes; of 676

predicted signaling molecules, only 167 were expressed in two or more of the transcriptomes.

This suggests that at least 167 of the transcripts predicted to encode a secreted signa ling protein

are expressed and are like ly functionally impor tant since they have been conserved across

mollusc species. As an additional method to validate expression of predicted transcripts, 15 of

the identified transcripts believed to encode signaling molecules were cloned from cDNAs

created from isolated Aplysia californica CNS’s.

It is likely that some of the remaining predicted transcripts may also be functionally

relevant but not highly conserved across species. This is not surprising given that the

comparison between Aplysia and Lottia neuropeptides reveals that those neuropeptides

responsible for development regulation share highly conserved transcript sequences while those

31

responsible for regulation are more highly variable. This may suggest one means for species

specificity at the neuronal level. Using recently released genomic data from Aplysia californica,

the intron/exon boundaries of neuropeptides were analyzed to determine if sequence

conservation and variability was due to the genomic arrangement of these genes. Preliminary

analysis of 5 conserved, divergent and moderately conserved neuropeptides does not reveal any

clear differences be tween the genomic organization of each type of neuropeptide.

Perhaps the most important finding is the large number of sequences with shared identity

found in both Aplysia and Lottia. These sequences represent a portion of the Aplysia secretome

that has been effective ly predicted without having to perform expens ive genomic sequencing or

repeat the computationally expensive process of secretome prediction. This is a major step in

bringing genomic-scale proteomics to a species without a sequenced genome.

Methods and Materials

Animals and Tissue Collections

Specimens of Aplysia californica weighing 150-280 g were collected in the wild by

Marinus Scientific (Long Beach, CA). Specimens of Pleurobranchaea californica, Clione

limacina, Tritonia diomedea, Melibe leonina, and Lymnaea stagnalis were obtained from the

Friday Harbor Labs, University of Washington. Octopus vulagris was obtained from Italy and

Nautilus pompilius from the Phillipines. Prior to dissection of gastropods, animals were

anesthetized by injecting a volume of isotonic MgCl2 (337mM) equivalent to 50%-60% of their

weight. Dissection of Cepholopods and Gastropod Mollusca was performed by Dr. Moroz.

Total RNA from whole CNS was extracted using RNAqueousTM (Ambion, Austin, TX) kit.

RNA isolation was performed by Dr. A Kohn, Jinnie Sloan, and Yelena Bobkova. Before

further processing of the RNA, quality was checked using a 2100 BioanalyzerTM (Agilent

Technologies). Small aliquots of extracted RNA were loaded on a 6000 Nano Lab chip that

32

produces an electropherogram image along with a gel- like image of the sample representing peak

ratios of the 28s/18s RNA contained in the sample. Additionally RNA concentration was

measured spectrophotometrically by using the GeneSpec III systemTM

454 Library Construction

(Mirai Bio).

Library construction was completed by Dr. Kohn and Jinnie Sloan. The protocol for

transcription analysis was developed by Drs. A. Kohn, Y. Panhin and L. Moroz.

3' end amplified cDNA library construction for 454 s equencing:

This technique targets the 3' end of the transcripts being expressed. Amplified cDNA was

generated using the Marathon cDNA amplification kit (Cat # 634913, BD Biosciences, Clontech,

Mountain View CA). The first strand synthesis utilized the AMV Reverse Transcriptase and an

oligo(dT) primer, Trsa (Matz, 2002). After second strand synthesis and c lean-up following the

Marathon kit protocol, the entire sample of cDNA was fractionated by digestion with 20 units of

Alu 1 and NEBbuffer 2 (Cat # RO137S0, New England BioLabs, Ipswich, MA) for 1 hour at

37°C. The enzyme was heat inactivated at 65°C for 20 min. The double stranded adaptor was

made with A adaptors then added to the ligation mixture at a final concentration of 1 µM along

with the digested cDNA, 2 Units T4 DNA ligase and 5 x ligase buffer (Cat # 634913, BD

Biosciences, Clontech, Mountain View CA). The ligation was performed at 16°C overnight.

The cDNA was purified using DNAclear (Cat#1756, Ambion Inc, Austin, TX) and eluted in 16

µl of RNAase-free water. Amplification of the cDNA was performed using 16 µl of the purified

cDNA, Advantage 2 buffer, dNTP and taq polymerase (Cat # 639201, BD Biosciences,

Clontech, Mountain View CA). The primers A pcr B pcr added to the amplification were at a

final concentration of 0.05 µM with B adaptor at a final concentration of 0.01 µM. This full

length B adaptor was modified to complement with the Trsa primer along with the B adaptor on

the beads and was added to ensure the eventual attachment of the cDNA on the beads. The PCR

33

amplification protocol consisted of two cycles of 95°C for 30 sec, 50°C for 30 sec, 72°C for 1

min, followed by 15 cycles of 95°C for 30 sec, 65°C for 30 sec, 72°C for 1 min. Half of this

PCR product was used for asymmetrical PCR to generate single strand cDNA. An excess of 10

times A pcr primer, final concentration of 0.5 µM was added along with 6 more cycles of 95°C

for 30 sec, 65°C for 30 sec, 72°C for 1 min performed. Controls were performed in which the

original PCR product was diluted 1:50 and 1µl used in a total volume of 20 µl. Only either A

pcr primer or only B pcr primer were added at a final concentration 0.05 uM and 15 cylces of of

95°C for 30 sec, 65°C for 30 sec, 72°C for 1 min were performed. This was to ensure that the

PCR-suppressive effect was occurring. The ssDNA was measured for size and concentration

then processed for bead attachment. This shotgun library was sequenced with GS Sequencing

Kit (70x75) (Cat # 04 853 342 001 Roche Applied Science).

5' end target amplified cDNA library construction for 454 s equencing:

This technique targets the 5' end of the transcripts being expressed and is very similar to

the previous protocol with a few minor revisions. Copied cDNA, first strand synthesis and

second strand synthesis were generated same as described. After second strand synthesis and

clean-up following the Marathon kit protocol, the entire sample of cDNA was ligated with the

double stranded A adaptors at a final concentration of 1 µM along with 2 units T4 DNA ligase

and 5 x ligase buffer (Cat # 634913, BD Biosciences, Clontech, Mountain View CA). The

ligation was performed at 16°C overnight. This cDNA was fractionated by digestion with 20

units of Alu 1 and NEBbuffer 2 (Cat # RO137S0, New England BioLabs, Ipswich, MA) for 1

hour at 37°C. The enzyme was heat inactivated at 65°C for 20 min. The cDNA was purified

using DNAclear (Cat#1756, Ambion Inc, Austin, TX) and eluted in 16 µl of RNAase-free water.

A second double stranded adaptor was made with B adaptors added at a final concentration of 1

µM along with the digested cDNA, 2 Units T4 DNA ligase and 5 x ligase buffer (Cat # 634913,

34

BD Biosciences, Clontech, Mountain View CA). This ligation was performed at 16°C overnight.

Again the cDNA was purified using DNAclear (Cat#1756, Ambion Inc, Austin, TX) and eluted

in 16 µl of RNAase-free water. Amplification of the cDNA was performed using 16 µl of the

purified cDNA, Advantage 2 buffer, dNTP and taq polymerase (Cat # 639201, BD Biosciences,

Clontech, Mountain View CA). The primers added to the amplification were at a final

concentration of 0.05 µM and the sequences were A pcr and B pcr. The PCR amplification

protocol consisted of 17 cycles of 95°C for 30 sec, 65°C for 30 sec, 72°C for 1 min. Preparation

and sequencing of the Amplicon product used the GS emPCR Kit II (Amplicon A and Paired

End) (Cat # 04 891 384 001 Roche Applied Science) GS Sequencing Kit (70x75) (Cat # 04 853

342 001 Roche Applied Science)

Primers for 3’ and 5’ libraries are listed in Table 2-4.

Cloning o f selected Signaling Molecules

Amplified cDNA libraries were constructed from the CNS of Aplysia californica, as

described elsewhere (Matz, 2002; Moroz et al., 2006). Full- length cDNA sequences for six

sequences and the partial sequence of an additional nine sequences were obtained. The full-

length copy of coding sequences were amplified from appropriate CNS cDNA libraries and

cloned into pC R 4-TOPO (Invitrogen). For each sequence, three clones were isolated and

sequenced by SeqWright (Houston,

Hypothetical protein 2

TX).

The full length sequence for Hypothetical protein 2 was identified based on BLAST results

against the Nationa l Center for Biotechnology Information (NCBI) as Aplysia californica

hypothetical protein previously cloned (Cummins et al., 2004) (Genebank accession number

AAN83922). Using terminal primers 5’-CTCTGAACCGTCGCGAACTGTGT-3’ and 5’-

35

AGGCAACAGTGGAATCGAAGCTCTC-3’, our lab cloned the full length sequence of

Hypothetical protein 2, resulting in a 788 bp fragment.

LFRFamide precursor

The full length sequence and clone for LFRFamide was obtained (Genebank accession

number EU886298) using terminal primers 5’-GGCCTCAGTTCGAAACCTCG-3’ and 5’-

ATTGGTCACCCTGTCCTCGG-3’. The resulting cDNA fragment was 1074 bp.

Myomodulin-like Neuropeptide Precursor 3

The full length sequence and clone for Myomodulin- like Neuropeptide Precursor 3 was

obtained (Genebank accession number EU934739) using terminal primers 5’-

CCTGAGTCCAGCCTAAGCGGTAAGT-3’ and 5’-GTACTGTGTAGGACGTAGGAAGCAG

-3’. The resulting cDNA fragment was 1690 bp.

Conopressin

The full length sequence and clone for Conopressin was obtained (Genebank accession

number FJ172359) using terminal primers 5’-CCAACTACAGGATGTCTCACTC-3’ and 5’-

GACGTTGAGTGGACACTGTGA -3’. The resulting cDNA fragment was 1983 bp.

Ependymin-related protein 2

The full length sequence and clone for Ependymin-related protein 2 was obtained (not

submitted) using terminal primers 5’-CTGGTATCAGAGCCACTCACCTC-3’ and 5’-

TGGTTGAATGTATACGTGCATGTAC -3’. The resulting cDNA fragment was 873 bp.

Betsin

The full length sequence and clone for Betsin was obtained (not submitted) using terminal

primers 5’-GTAACTTTCGTCCCTCCTGCCA-3’ and 5’-CTTCTACTTGACCCACTCGGACC

-3’. The resulting cDNA fragment was 538 bp.

36

Feeding circuit activating petide precursor 3 (FCAP-3)

The partial sequence and clone for FCAP-3 was obtained (not submitted) using terminal

primers 5’-AACAGGCGCAGGCTCAGA-3’ and 5’-ACACGTGGATCGCCGCCGCC -3’. The

resulting cDNA fragment was 367 bp.

SN4

The partial sequence and clone for SN4 was obtained (not submitted) using terminal

primers 5’-ATGCAGTGAGGGATGCTGTGT-3’ and 5’-GGTCAAGGCCAATTTCCCGTG -

3’. The resulting cDNA fragment was 755 bp.

SFY1-like peptide

The partial sequence and clone for SFY1-like peptide was obtained (not submitted) using

terminal primers 5’-GTGGAAGTACGCAGAGAGG-3’ and 5’-CATCCCTCTTGTAGAAGCTGGA-3’.

The resulting cDNA fragment was 1198 bp.

SFY3-like peptide

The partial sequence and clone for SFY3-like peptide was obtained (not submitted) using

terminal primers 5’-GTATCAAGACCGACGACCATG-3’ and 5’-GTGAGCTCATCCCGCGGTTG-3’.

The resulting cDNA fragment was 729 bp.

FIRFamide related neuropeptide precursor

The partial sequence and clone for FIRFamide related neuropeptides precursor was

obtained (not submitted) using terminal primers 5’- CGTCATCGCTGGTGCTGTCAC-3’ and 5’-

CCTCGACAAGGCTTCTCCTTCACC-3’. The resulting cDNA fragment was 2032 bp.

Major royal jelly protein (MRJ)

The partial sequence and clone for MRJ protein was obtained (not submitted) using

terminal primers 5’-CGGAAGTCCGGTACGCGTATATTTC -3’ and 5’-

CTTTCAGAAGGCTATTCCTCCCACC -3’. The resulting cDNA fragment was 774 bp.

37

FMRFamide 5

The partial sequence and clone for FMRFamide 5 was obtained (not submitted) using

terminal primers 5’-GGTGGATCAAGCCTTGGAGCT-3’ and 5’-CACTCAGGTTATTCAACACGTCAAC-


Allatotropin-OR precursor

The partial sequence and clone for Allatotropin-OR precursor was obtained (not submitted)

using terminal primers 5’-CAAGTGGTGATTCGGCCGC-3’ and 5’-GTCAATATAGTTCCCTCTCGTGG-


Second

The partial sequence and clone for Second was obtained (not submitted) using terminal

primers 5’-GCTAATGAGCGATTTCTGCGAG-3’ and 5’-CGTCCTTCTCCGAAAGGCCTAAGCC-3’. The

resulting cDNA fragment was 712 bp.

Neurosecretory Predictions

Preditions were performed by Jinnie Sloan, Mathew Citarella, Drs. A. Kohn and L. Moroz.

Software scripts were written by M. Citarella.

Homology search using unbiased shotgun approach

A cross species master list of secreted signaling molecules submitted to NCBI was created

by manual word search. Search terms included any combination of the following words: signal,

peptide, growth factor, hormone, and neuropeptide. Any results containing the word “receptor”

were eliminated.

All sequences submitted to PeptideDB (www.peptides.be, May 2009), a public resource

for bioactive peptides that includes cytokines and growth factors, peptide hormones,

antimicrobial peptides, toxins and venom peptides, and antifreeze proteins, were manually

38

downloaded and added to the above results in FASTA file format. All redundant sequences were

removed and the master list was aligned with cDNA ESTs from Aplysia as described below.

To ensure that all results represent SSMs, all Aplysia homologs were screened using

SMART (Schultz et al., 1998), and any sequences with a transmembrane domains or enzyme

protein family domains were removed.

Genomic approac h

To facilitate prediction of the Lottia secretome, 23,851 full length protein sequences were

downloaded from the Joint Genome Institute (JGI) website (www.jgi.doe.gov, May 2009).

These sequences represent the best filtered protein-coding gene models as determined by JGI’s

genomic assembly. The latest release of NCBI’s non-redundant protein database, NR, was

downloaded to provide annotation for any predicted secretory products. NR contains protein

sequence entries from the following databases: GenPept, Swissprot, PIR, PDF, PDB, and NCBI

RefSeq.

Classical Secretory Prediction*

The original data set of 23,851 full length protein sequences from the Lottia genome was

analyzed with TargetP 1.1. Batches of 1000 sequences were fed to TargetP via a custom

wrapper script (Citarella, M.R. unpublished), written in Perl using the ‘short’ output op tion for

TargetP. Sequences were considered to be targeted to the secretory pathway if they had a SP

(secretory pathway) score > .80. All other sequences were discarded from the prediction. The

selected sequences were then analyzed with SignalP 3.0 for the presence of a signal sequence

using both Neural Network and Hidden Mark Model methods and the ‘short’ and ‘no-graphics’

output options. Proteins were determined to have a signal sequence if SignalP returned a “Y” for

four out of the five scores for the Neural Network method a nd the Hidden Markov Model

method predicted that it contained a signal peptide. Transmembrane domains were then

39

predicted using TMHMM 2.0c4

Non-classical Secretory peptide Prediction*

using the default settings. Only sequences with no predicted

transmembrane domains or one transmembrane domain and more than four residues of that

domain in the first 60 amino acids were considered further (these proteins are retained since a

single predicted transmbrane domain in the first 60 amino acids of a protein may be a signa l

sequence falsely identified as a transmembrane domain). These proteins were analyzed with the

web version of Phob ius using the “short” option. Proteins with no predicted transmembrane

domains and a confirmed signal sequence were selected as the final predicted classically secreted

proteins.

14,595 full length protein sequences that were localized to ‘other’ with a score > .75 by

TargetP during classical secretory peptide prediction were analyzed with the web version of

SecretomeP 2.0 in sets of 100 with the “mammalian” option checked. Sequences were

determined to be secreted via a non-classical pathway if their NN score exceeded .75 and there

was no predicted signal peptide by SignalP.

*Unless otherwise noted, the above predictions were all performed with the standalone version of the software package mentioned on an Intel Pentium 4 with 1GB RAM running Ubuntu Linux 8.04.

Cross-Species Analysis of Predicted Proteins

All homology searches including the final set of classically and non-classically secreted

proteins from Lottia were aligned with cDNA Expressed Sequence Tags (ESTs) from Aplysia

californica CNS using NCBI’s standalone BLAST package. The following opt ions were set –

program: blastp, e-value: 1e-04, processors: 3, word-size: default(3). The results of each BLAST

were parsed with a custom Perl script, findHomologs.pl. For each predicted secretory protein, a

homolog was said to be found in a species if there was a BLAST hit for that species with an e-

40

value less than or equal to 1e -04. Furthermore, a single sequence from a given species could be

reported as a homolog for at most one Lottia secretory protein.

Annotation of Predicted Proteins

To annotate the list of predicted secretory proteins in Lottia, the latest release of NCBI’s

NR database was downloaded and each predicted protein was BLASTed against it using NBCI’s

standalone package set for blastp with default settings. Annotation for a given predicted protein

was determined as the identifier of the sequence from the NR database with the lowest e-value

hit to the predicted protein, so long as the e-value was less than or equal to 1e-04.

41

Figure 2-1. Summary of model organism and number of ESTs/cDNAs collected. More than 980,000 ESTs/cDNAs were collected from the CNS of five key model species (Pleurobranchaea californica, Clione limacina, Tritonia diomedea, Melibe leonina, and Lymnaea stagnalis). Here, the number of ESTs for each species is shown under their name.

Nudibranchia

Melibe leonina

Tritonia diomedea

Aplysia californica

Clione limacina

Gastropoda Lymnaea stagnalis

Lottia gigantea

Pleurobranchaea californica

Acanthopleura spinosa

(259,277)

(131,851)

(115,599)

(10,809)

(273,922)

(220,000/2,392,545)

(188,590)

Melibe leonina

(259,277)

(131,851)

(115,599)

(10,809)

(273,922)

(220,000/2,392,545)

(188,590)

Melibe leonina

(259,277)

(131,851)

(115,599)

(10,809)

(273,922)

(220,000/2,392,545)

(188,590)

Melibe leonina

(259,277)

(131,851)

(115,599)

(10,809)

(273,922)

(220,000/2,392,545)

(188,590)

Opisthobranchia

Polyplacophore

Pulmonata

Prosobranchia

42

1.00E-116

1.00E-107

1.00E-98

1.00E-89

1.00E-80

1.00E-71

1.00E-62

1.00E-53

1.00E-44

1.00E-35

1.00E-26

1.00E-17

1.00E-08

1.00E+01

Dor

sal-v

entr

al

Buc

calin

Ped

al p

eptid

e 1

Con

opre

ssin

Epen

dym

in

Tem

ptin

Prot

hora

cico

stat

ic

Myo

mod

ulin

Pleu

rin

Whi

tnin

Inte

rsec

tin-2

insu

lin

PTSP

-like

Neu

rope

ptid

e Y

SN

4

Ent

erin

Tollo

id 2

Orc

okin

in

Ven

om p

rote

in 2

FMR

Fam

ide

Ful

icin

-like

MIP

-rel

ated

Neu

roto

xin-

1

LFR

Fam

ide

Neuronal Transcript

Figure 2-2. A nalysis of Evolut ionary Dynamics of Neuronal Transcripts. Preliminary analysis suggests that some neuropeptides may be fast evolving molecules. The sequence conservation between Aplysia and Lottia reveals that proteins involved in development (i.e. Dorsal-ventral patterning) have the highest sequence similarity (indicated by a high e-value from sequence alignments), while neuropeptides involved in regulatory mechanisms (i.e. FMRFamide) have the most variability. These likely reflect differences in biological constraints between these two neurona l processes. The fast evolut ionary divergence of some neuronal genes may account for the large amount of unannotated sequences from the neuronal transcriptome of Aplysia (insert).

E-value Aplysia californica 203,440 transcripts

43,672 annotated

159,768 unannotated

43

FMRFamide

Internal Repeats Intrinsic Disorder

Signal Peptide

Figure 2-3. Conserved Signaling Molecule Motif. Example of the conserved motifs of secreted signaling molecules (here the neuropeptide FMRFamide is shown). Secreted signaling molecules are targeted to the secretory pathway by their signal peptides, and often are characterized by intrinsic disorder, internal repeats, and cysteine rich regions. They are differentiated from proteins encoding receptors by a lack of transmembrane regions.

44

Figure 2-4. Workflow for Computational Prediction of SSMs. The filtered gene models for Lottia gigantea were downloaded from JGI. After filtering the original 23,851 predicted proteins through four protein prediction software programs, 859 proteins were predicted to be classically secreted signal molecules. That is, contained signal sequences, no transmembrane domains, and were targeted for secretion outside the cell. Using BLAST alignments, 400 c lassically-secreted homologs were identified in Aplysia ETSs.

45

Table 2-1. Annotation of Lottia predicted secretory products against NCBI’s NR database. The results of the annotation show the ~24% of the classically predicted secreted peptides cannot be mapped to a biological function due to poor annotation. This could be a product of the evolut ionary distance be tween Lottia and ot her commonly sequenced organisms. If the species represented by the sequences in NR are divergent enough from Lottia, the predicted proteins will not be similar enough to the database entries to provide significant hits for annotation.

Data Set Number

Annotated Number Unannotated

Percent with ‘Predicted’ in Annotation

Percent with ‘Hypothetical’ in Annotation

Percent of Peptides with unknown or ambiguous functions

Classically- Secreted Peptides

83 14 46.53% 5.94% ~24%

46

Table 2-2. Summary of Cross Species Predictions. Results of cross species predictions using homology search against nonbiased shotgun transcriptomes and homology search against our Lottia genomic predictions. Results show that more predicted neurosecretory products are found using the genomic approach perhaps suggesting a genomic approach as a more robust form of predicted signa ling molecules in gastropod molluscs. It is unclear whether differences across species represents sequence divergence from the model organism used (Lottia) or is simply an artifact of different levels of coverage of the sequencing for each species.

Species Shotgun Genomic Object Aplysia californica 90 74 2-1 to 2-3

Lottia gigantea 75 87 2-4 to 2-5

Lymnaea stagnalis 66 48 2-6

Pleurobranchaea californica 45 42 2-7

Tritonia diomedea 37 28 2-8

Melibe leonina 18 14 2-9

Clione limacina - 9 2-10

Octopus vulgaris - 31 2-11

Nautilus pompilius - 44 2-12

47

Figure 2-5. Comparative analysis of predicted Lottia secretory products. The percentage of all predicted Lottia secretory proteins for which homologs were found in each species. While some variation in expression of homologous sequences may be due to the number of ESTs collected, it may also provide insight to the evolutionary distance between species. These data suggests SSMs between Lottia and Aplysia are the most conserved, followed by Lymnaea and Pleurobranchaea. Furthermore, classically and non-classically secreted proteins appear to evolve at different rates across species with non-classically secreted peptides being more highly conserved.

Aplysia Clione Lymnaea Melibe Pleurobranchaea Tritonia

48

Table 2-3. Genomic organization of neurosecretory genes. Preliminary analysis of the genomic

organization of selected neurosecretory products shows that all genes have a similar intron/exon organization, with an average of 3-5 exons. (See Supplemental A for detailed information.)

Neuropeptide Number Introns

Number Exons

Atrial gland-specific antigen precursor 9 10 Feeding circuit activating peptide precursor 1 2 FMRFamide neuropeptide precursor 2 3 L11 neuropeptide precursor 2 3 Buccalin precursor 3 4 Conopressin 3 4 Fulicin-like neuropeptide precursor 4 5 MIP-related peptide precursor 4 5 Neurotoxin-like-1 3 4 Pleurin 2 3 Whitnin precursor 2 3

49

Table 2-4. Adaptors and Primers for 5’ and 3’ 454 libraries Primer Name Primer sequence Trsa 5'-CGCAGTCGGTAC (T)13 -3' 3’ bias library A Adaptor 5'-CCATCTCATCCCTGCGTGTCCCATCTGTTCCCTCCCTGTCTCAG-3'

and 5'-CTGAGACAGGA-3' A pcr 5'-CCATCTCATCCCTGCGTGTC-3' B pcr 5'-CCTATCCCCTGTGTGCCTTG-3' B adaptor,Trsa 5'-CCTATCCCCTGTGTGCCTTGCCTATCCCCGCAGTCGGTACTTTT-3'). 5’ bias library A Adaptor 5'-GCCTCCCTCGCGCCATCAG-3' and 5'-CCTGATGGCGCGAGGG-3' B Adaptor 5'-GCCTTGCCAGCCCGCTCAG -3' and 5'-CTGAGCGGGCTGGCA-3' A pcr 5'- GCCTCCCTCGCGCCATCAG -3' B pcr 5'- GCCTTGCCAGCCCGCTCAG -3'

50

CHAPTER 3

MAPPING EXPRESSION OF NEUROPEPTIDE TRANSCRIPTS

Introduction

One major function of neuropeptides in both vertebrates and invertebrates is to act as

extracellular chemical messengers to modulate the communication between neurons of the

central and pe ripheral nervous systems (Kaldany et al., 1985; Strand, 1999). Physiological

studies in Aplysia californica have demonstrated peptidergic modulation plays a crucial role in

the initiation of several behaviors including feeding, egg laying, and cardioregulation

(Campanelli and Scheller, 1987; Scheller et al., 1983; Sossin et al., 1987; Sweedler et al., 2002).

Several families of neuropeptides are expressed in molluscs (Fujiwara-Sakata and Kobayashi,

1992; Price and Greenberg, 1977; Smit et al., 1991) as well as individual neuropeptides,

(Banvolgyi et al., 2000; Kuroki et al., 1990; Smit et al., 1992).

To support the identification of putative signaling molecules identified in the first chapter

of this thesis, in situ hybridization was performed for a select number of signaling molecules to

test for expression of the RNA transcript. Here, the expression of two putative neuropeptides,

MIP and Fulicin, is described for the CNS of Aplysia.

Mytilus Inhibitory Peptides

One set of identified neuropeptides in Aplysia belongs to the Mytilus inhibitory peptides

(MIPs) family (Fujisawa et al., 1999). Originally isolated from the pedal ganglia of the bivalve

Mytilus edulis (Hirata et al., 1987), MIPs can inhibit target muscles and hyperpolarize central

neurons (Kiss and Osipenko, 1997; Kissler et al., 1997; Yongsiri et al., 1989). Aplysia MIP-

related peptides (AMRPs) are expressed in the CNS and peripheral tissues including the

digestive tract, vasculature, and reproductive organs, where they have a dose-dependent

inhibitory action on target tissues (Fujisawa et al., 1999; Hirata et al., 1987).

51

Fulicin

Previously unidentified in Aplysia, Fulicin was initially isolated from the African giant

snail Achatina fulica (Ohta et al., 1991). In Achatina Fulicin has been shown to regulate female

egg- laying behavior, potentiate tetanic contraction of the penis retractor muscle and modulate

actions of the ganglionic neurons as well as buccal and ventricular muscles (Fujisawa et al.,

2000; Ohta et al., 1991). F ulicin was shown to share the C-terminal portion Phe-Val-NH2 with

Mytilus inhibitory peptides (MIPs), and both peptides depress the phasic contraction of the

ABRM muscle in Mytilus edulis (Hirata et al., 1988; Kim et al., 1991). However, Fulicin was

shown to be 10,000 times less potent than MIPs, presumably due to the lack of Pro-residue

required for inhibitory activity of MIPs (Kim et al., 1991).

This chapter focuses on the mapping of these two neuropeptides exclusively to provide

further insight into possible explanations for the shared C-terminal region of these two protein

precursor. Here we cloned, localized, and show predicted gene products of an Aplysia Fulicin-

related peptide (AFRP). Using two-color in situ hybridization (Jezzini et al., 2006), we show co-

localized expression of MIP and Fulicin transcripts in the CNS of Aplysia. Finally, we postulate

that the shared region of MIP and Fulicin, TxFrag2, is a regulatory genomic element that

provides a molecular mechanism to regulate expression of two neuropeptides in a single neuron.

Results

Cloning o f Aplysia Fulicin precursor mRNA

As described in Chapter 1 of this work, a putative neuropeptide Fulicin was identified

during annotation of transcripts generated from unbiased shotgun sequencing. The identified

Aplysia Fulicin- like transcript was cloned resulting in a ful l lengt h sequence with a 1059 base

pair open reading frame coding for a 352 amino acid precursor (Figure 3-1A).

52

The predicted Fulicin precursor protein contains a hydrophobic signal peptide and a

cleavage site between Asp and Thr, which suggests this protein is targeted to the secretory

pathway. Analysis of monobasic, dibasic and tribasic cleavage sites suggest that there are 13

copies of 10 different predicted amidated peptides processed from the Fulicin precursor (Figure

3-1B).

Localization of Fulicin in the CNS of Aplysia

Since Fulicin has not been localized to the CNS of Aplysia we first wanted to map

expression of Fulicin using in situ hybridization. The Fulicin transcript showed neuron specific

expression with the most intense staining in specific neurons of the abdominal and pleural

ganglia (Figure 3-2). Interestingly, Fulicin clearly labels the L7 motor-neuron in add ition to

other motor neurons critical to the function of the gill and siphon withdraw circuit. Expression is

localized in the LP1 neuron of the pleural ganglia, and small subsets of neurons in the cerebral,

pedal and buccal ganglia, suggesting that Fulicin expression is not ubiquitous. It was noted that

many of the neurons pos itive for Fulicin expression are the same neurons known to express MIP,

suggesting that besides sequence similarity, the expression of these two neuropeptides may also

be similar.

Co-localization of MIP and Fulicin

To further investigate the possible co expression of MIP and Fulicin in the CNS of Aplysia,

co-localization was performed using two-color in situ hybridization (Figure 3-3). Using two-

color in situ hybridization labeling with specific probes we found that MIP and Fulicin were

exclusively co-expressed. During the staining protocol it became apparent that some cells do

stain with different intensities, suggesting that the expression level of these peptides may not be

uniform in positively labeled cells, but rather expression levels are neuron specific.

53

Isolation of Fulicin in the CNS of Aplysia

454 sequencing revealed higher expression of transcripts aligning to the coding regions of

the MIP gene than real time PCR (RT-PCR) experiments (Table 3-1). We found that MIP

showed strong sequence similarity to another gene, Fulicin, in its 5’ region (Figure 3-4) as

supported by previous work in Mytilus edulis showing MIP and F ulicin have a structurally

similar C-terminal region (Hirata et al., 1988; Kim et al., 1991).

MIP and Fulicin share 5’ UTR and coding region

We postulate that the shared 5’ untranslated region may serve as a molecular mechanism

underlying the observed colocalization of these two neuropeptides. To understand the

functional role of this shared region, we extended the sequence of the coding region into the 5’

UTR of each of these genes to check if the shared sequence continued upstream of the protein

start site.

Using 5’ RACE, we extended the non-coding region of both Fulicin and MIP to reveal a

480 nt region, called TxFrag 2, that includes the 5’ UTR and the 21 amino acid signal peptide of

MIP and Fulicin (Figure 3-1A, blue and Figure 3-5).

TxFrag2: Structure and Function

Alignment of TxFrag2 to genomic data available on NCBI reveals that the 480 nt region is

transcribed from four defined fragments of DNA separated by three intergenic splice sites,

characteristic of transgenic noncoding RNA. The fourth defined fragment contains the signal

sequence shared by both MIP and Fulicin. At the end of the signal sequence there is a splice site

in both MIP and Fulicin, the start of Exon 2 marks the beginning of the unique coding region of

both genes.

Previous work mapping short noncoding RNAs (sRNAs) indicates sRNAs cluster at the 5’

and 3’ of genes. Similar to these results, Transfrag2 is located at the 5’ of MIP and Fulicin. Like

54

other intergenic sRNAs (IRNAs), TxFrag2 includes the 5’ boundary of the protein-cod ing gene

but excludes most of the other exons of the coding region. Previous work on IRNAs suggests

they are involved in regulation of gene expression (Davis et al., 2006; Martianov et al., 2007).

These studies suggest that IRNA transfrags serve as precursors to functional sRNAs via sense

and antisense transcription of the IRNA (Kapranov et al., 2007).

To see if TxFrag2 is transcribed in the sense or antisense direction, 454 and SOLiD

transcripts that aligned to the 480 nt TxFrag2 region were counted and compared to the

quantities of sense and antisense MIP and Fulicin (Figure 3-5B). This showed that TxFrag 2 is

transcribed in both the sense and antisense direction where as the coding region of MIP and

Fulicin are made only in the sense direction.

Discussion

Colocalization of Fulicin and MIP

This is a unique example of two neuropeptides showing exclusive colocalization

throughout the CNS of Aplysia. After several in situ experiments, we have found that transcripts

for both MIP and Fulicin consistently colocalize to specific neurons of the CNS of Aplysia,

predominately those neurons in the abdominal ganglia responsible for the gill and siphon

withdraw.

Analysis of the protein sequence of MIP and F ulicin reveal that structurally, they share a

conserved C-termina l. F urther ana lysis of the nucleotide cod ing region of the sequences shows

that this results from a conserved 5’ UTR region that extends through the first exon of both

genes. Preliminary analysis using recently released genomic information from Aplysia

californica further suppor ts that MIP and Fulicin share the same splice sites and exon regions

covering the TxFrag2 region until after the signal sequence.

55

There is currently one additional publication indicating that the preproprotein of two

neuropeptides, brandykinin and temporin in frog, share a conserved 5’ UTR and signal sequence

(Suzuki et al., 2007). In this paper the authors suggest that the sequence similarity may suggest a

linked evolutionary history involving exon shuffling.

TxFrag2: Modulator of Expression?

With the completion of several genome projects, including the Human Genome Project,

the once accepted view that of noncoding RNAs (ncRNAs) as “junk” is rapidly shifting to accept

non-protein-coding transcripts are crucial for cellular function. Recent reports suggest that only

2% of the human genome codes for translated proteins (System, 2008) while nearly half of the

genome is transcribed and expressed as RNA without any messenger (mRNA), transfer (tRNA),

or ribosomal (rRNA) functions (Szell et al., 2008). Understanding the role of these ncRNAs

represents a new realm of understanding genomic data, and the accumulating data suggests

ncRNAs may play a role in organism complexity, specificity, cell regulatory machinery, and

regulation of these ncRNAs has been implicated in several human diseases (Szell et al., 2008).

Work on the human genome has demonstrated that the transcriptome does not exist

exclusively of protein-coding transcripts but also regulatory genomic elements and non-protein-

coding transcripts (Szell et al., 2008). This project, termed ENCODE (the Encylopedia of DNA

Elements), along with unbiased tiling-array data has identified a new set of transcripts (TxFrags)

that are made from fragments of defined genomic regions. Cross species genome sequencing has

shown that increasing biological complexity is correlated with increasing number of non-protein-

coding DNA sequences (Taft et al., 2007) and suggests that differences between species may rely

upon non-cod ing genes rather than protein-coding genes (Pollard et al., 2006).

While the function of TxFrag2 remains to be determined, e xpression of bo th sense and

antisense transcripts suggest that it may serve in a regulator mechanism in these cells. It is our

56

hypothesis that TxFrag2 may function similar to miRNAs, regulating the expression of MIP and

Fulicin by binding antisense transcripts to complementary upstream translation regions.

However, the large size of TxFrag2, 480nt, compared to a normal miRNA, ~20nt, challenges any

conclusions about the mechanism of TxFrag2 regulation. Overall, these data provide evidence

for a possible molecular mechanism underlying how neurons can regulate expression of multiple

neuropeptides within a single cell.

Materials and Methods

Animals

Specimens of Aplysia californica weighing 150-280 g were collected in the wild by

Marinus Scientific (Long Beach, CA). Animals were anesthetized by injection of 50%

(volume/body weight) isotonic MgCl2 (337

Cloning o f full-length cDNA encoding Fulicin

mM) prior to surgical removal of the central nervous

system (CNS).

Terminal primers were designed from two overlapp ing ESTs that shared high identity to

mRNA for the Fulicin precursor in Achatina fulica (Genebank accession number D13986).

A full- length cDNA sequence called fulicin- like neuropeptide precursor (Genbank

accession number AAW30458) was obtained using terminal primers: 5'-

CAATCAACCCGCAATGTGTACC -3'

Sequence analysis and Alignments

and 5'- CTAAGAATCCGGGCACGACGC -3' from an

amplified cDNA library. The amplified PCR product was 1064 bp.

The initial multiple alignment was done using ClustalX ver. 1.83 (Jeanmougin et al., 1998;

Thompson et al., 1997) with default parameters. All protein predictions were determined with

Pros ite (Gattiker et al., 2002) and SMART (Letunic et al., 2006).

http://www.ncbi.nlm.nih.gov/nuccore/D13986�

57

In-situ hybridization of Fulicin and MIP in Aplysia

Full- length cDNA from Fulicin and MIP was cloned and used for the preparation of in s itu

probes. For colocalized expression studies, clones were made for Fulicin and MIP from unique

regions starting after shared 5’ UTR and signal sequence. The antisense probe was generated by

digestion of cDNA from Fulcin and MIP with Not I (New England Biolabs), then transcription

with T3 polymerase from the DIG (digoxigen) RNA labeling kit (Roche Diagnostics). The

control sense probe was produced by the same protocol but used Pme1 (New England Biolabs)

to digest the cDNA and T7 polymerase for transcription. The DIG-labeled antisense probes were

hybridized in whole-mount CNS preparations, and the neurons containing the probe-target

duplex were localized and visualized with alkaline phosphatase-conjugated anti-DIG antibody

fragments (Boehriger Mannheim). The detailed in situ hybridization protocol has been described

(Jezzini et al., 2005; Jezzini and Moroz, 2004; Walters et al., 2004).

Expression of Fulicin was investigated in central ganglia of four experimental CNS

preparations and two control experiments. Control in situ hybridization experiments with full-

length "sense" probes revealed no specific and selective staining in the CNS under identical

conditions and labeling protocols for either probe.

Co-localization using two-color in-situ hybridization was performed as previously

described (Jezzini et al., 2005). Briefly, unique regions of MIP and Fulicin were cloned and

sequenced. Two probes were then made using different NTP labeling mixes. The MIP probe

was made using fluorescein-12-UTPs and the Fulicin probe was DIG-labeled. F irst the

fluorescein-MIP probe was hybridized in whole-mount CNS preparations, and the neurons

containing the probe-target duplex were localized and visualized with Fast Red substrate. After

the first development is stopped, a second hybridization is preformed using the DIG-Fulicin

58

probe and the neurons are localized and visualized with alkaline phosphatase-conjugated anti-

DIG antibody fragments (Boehriger Mannheim).

Imaging

Images were captured with a Nikon Digital Sight DS-5M digital camera mounted on an

upright Olympus SZX12 microscope. Figures were prepared using Adobe Photoshop.

59

Figure 3-1. Fulicin Gene and Protein Predictions. A) Full length open reading frame and UTR for Aplysia Fulicin- like protein. Nucleic acids and amino acids are numbered at left and predicted amidated peptides are shown in red. Monobasic, dibasic, and tribasic cleavage sites are shown in green. The signa l sequence is shown in orange. TxFrag2, shared by both MIP and Fulicin is shown in blue. B) Predicted amino acid sequence of Fulicin. Cleaved peptides are predicted by look ing for conserved c leavage sites (shown in green in 3-1, A).

B. F T E F L

Q W E F V

P Y D F V

Y D F V

Y D F L

P Y D F L

S Y D F L

P Y E F I

P Y E F V

Y A E F L

A. 1 GCTGGTAACGAGCCCTGAATACTGGACAATACAACAGAGTTGGAGAAACAGATGTGTGTA ┌ Splice 1 61 GCATGATGAAGTAGACACCGGTCAAGAGAGTAACACCACCGGAAACTGACCATAGAATTC 121 CAAAAACTCTGTGTTCAAAATAACTAGTGGCGGACTTTCACGAAATCTGATCTCCACACG 181 TCGAAGAGCGAATACCTGCAGTGACGAGCGTCGGTTTCCCTTCAACTCATTCTTGTCATT ┌ Splice 2 ┌ Exon 1 241 TTCATCTTGAAGTGGTTCAGGTTACCACAGAAAGAAAGACAAGAATTGTACTGATTTTTT 301 CTGAGTTCTAATTCCAATCAACCCGCAATGTGTACCCGACCTGGCCTGGCTGCTCTGCTG 11 M C T R P G L A A L L ┌ Exon 2 361 GTGCTGATGACGTCTTGTGCCTCCAGCTTCTCACGTGCAGATACCACGAAGACTGGACAC 31 V L M T S C A S S F S R A D T T K T G H 421 CTGAAAAGGCCAACCCGATCAAGTGACCAGTTTTTCGACAGGAGCGACACGGAACCTCTC 51 L K R P T R S S D Q F F D R S D T E P L 481 CAGCCCCCAAACAGTGACCAGTACCTCTCTTCATTGAAAAACAAAGGCCAGTTCAGCAAC 71 Q P P N S D Q Y L S S L K N K G Q F S N 541 GAGCAATATCCTGGAGAGTATTTTGACGGGTCCGGGTTCAACGCCGAGGAGGAAGAAAGG 91 E Q Y P G E Y F D G S G F N A E E E E R 601 CCTCTACATCAAGGGGATGGGGACAGTGTGTCACTTTTGGGAGACAGCCCTCCGAGCCCG 111 P L H Q G D G D S V S L L G D S P P S P 661 GCCATGTCGAAAAGATTTACAGAGTTCCTAGGTAAAAGGGTGCCTTTGGCTAACAGTGGG 131 A M S K R F T E F L G K R V P L A N S G 721 ATGCCTGGCCCTGTGGAAGAGCCACACAAGGAGACCGTAGAGGATAGTCATAGGTCAGAG 151 M P G P V E E P H K E T V E D S H R S E 781 GGGTTATCACCATCAGAAAGATCGTATAGAGACATGGCAGAAAAGCTGGCTTTACTACTC 171 G L S P S E R S Y R D M A E K L A L L L 841 CGCTCTGACGCCAAAAGACAGTGGGAGTTTGTCGGAAAGCGACCATACGACTTCGTAGGC 191 R S D A K R Q W E F V G K R P Y D F V G 901 AAAAGATACGACTTTGTAGGCAAGCGATACGACTTTGTTGGCAAAAGGTACGACTTTTTG 211 K R Y D F V G K R Y D F V G K R Y D F L 961 GGAAAGAGAAATCCGTACGAGTTTATTGGGAAGCGGTACGACTTTGTGGGAAAACGTCGC 231 G K R N P Y E F I G K R Y D F V G K R R 1021 CCCTACGACTTTCTAGGGAAAAAGAGTTACGACTTCCTCGGGAAAAGGTACGACTTTTTG 251 P Y D F L G K K S Y D F L G K R Y D F L 1081 GGAAAAAGGAATCCGTATGAATTCGTTGGTAAAAGAACTCCGGCTATGGTTCATGAAGGC 271 G K R N P Y E F V G K R T P A M V H E G 1141 GTGATCTCACACAACCTAGACGGCGAGGGTCAAAAACTGGCCATTCCATCCTCTGATGCC 291 V I S H N L D G E G Q K L A I P S S D A 1201 CACACCGCAGACAGAAGGTACGCCGAGTTTCTGGGCAAAAGGTCCGAAGAAAGTGGACAA 311 H T A D R R Y A E F L G K R S E E S G Q 1261 GCAGCTCTGACAGACAGCGCCCGTCTGGCCGCTCTACTCAGCAACACTGGCCTGAGGAAA 331 A A L T D S A R L A A L L S N T G L R K 1321 CGGCTGTCCAGAATGCTTCTGAACGGTCAGCTGGCGGAACAGTACCCAGAGTTTATTGGG 351 R L S R M L L N G Q L A E Q Y P E F I G 1381 AAATAGGACACGGTATTTTCTAAGAATCCGGGCACGACGCAAAACTGTTATAGAAATCAC 371 K * 1441 TTCCACTGAGTTAATCCAAAAGTTACGAAGGTAAATGTAGTTTCTATCCTGCGAACCGCG 1501 TAGTATGCATGTTAACGACGACAACAACTGCAAATGCATAGGTTTATGTATTAATCATAT 1561 GCATTACTCAAAAGATCAAACTGAAAATTCTAGTATCATATATTATTATCTACTAACAAA 1621 TCATGCCTTACTTCTTCTTGAACTTTGTAGTTTTCATTTTTTTTAATAGACCTGTTTAAT 1681 TTGAGAAACAATCGTATATAGAATGCCAGACTTCTCAATAAAAAGTGGGTTATAATCATA 1741 AAAAAA

60

Figure 3-2. Schematic overview of the distribution of Fulicin- like transcript in the CNS of Aplysia. Each circle represents a single cell showing staining for the Fulicin- like transcript. Positions of identified cells MCC, R2, and LP1 are indicated, (viewed from caudal surface). Most significant staining was seen in the Abdominal and Pleural ganglia.

Cerebrobuccal connective

MCC

B1 B2

B5 B6

B4

SC SC

C1

C2

C3 C4

C5 E Cluster

E Cluster

B Cluster A Cluster

Pleuroabdominal connectives

Pec


R3 - R13

R2 A6

A5 A4 A2

BC BC

LP1

Cerebropedal connective

Cerebropleural connective

P5

P6

P7

P9 P8

P5

P4

P6

P9

P8

Cerebrobuccal connective

MCC

B1 B2

B5 B6

B4

SC SC

C1

C2

C3 C4

C5 E Cluster

E Cluster

B Cluster A Cluster


Pec


R3 - R13

R2 A6

A5 A4 A2

BC BC

LP1

Cerebropedal connective

Cerebropleural connective

P5

P6

P7

P9 P8

P5

P4

P6

P9

P8

Pleural ganglia

Abdominal ganglia

61

Figure 3-3. Colocalized expression of MIP and Fulicin transcripts. MIP-specific staining shown in red, Fulicin-specific staining shown in blue. A) and B) the gradual co-localized development of Fulicin and MIP in the Abdominal ganglia. C) Cells in the Pleural ganglia, including LP1 show colocalized expression.

A

B C

62

Table 3-1. Comparison of Real Time PCR of Aplysia MIP-related gene and 454 sequencing.

Comparison of 454 s equence frequency to RT-PCR reveal significantly higher levels of expression of the MIP gene, suggesting t hat another gene may be expressed that has sequence similarity to MIP.

TranscriptCopy Number(Average)

5’Reads 5’Frequency(1,010,896 total)

3’Reads 3’Frequency(468,723 total)

MIP(coding AF454399.1)

2.12E+05 9295 9.19E-03 170 3.63E-04

63

Signal Sequence

Figure 3-4. Alignment of the coding region of Aplysia MIP- like protein against the identified Aplysia Fulicin- like protein. Both share complete identity at their 5’ ends, including the signal sequence. It is also noted that the transcripts share repetitive regions at their 3’ ends.

64

A

B

Figure 3-5. Intergenic splicing and expression of TxFrag2. A) TxFrag2 is composed of 3 intergenic splice sites and 1 intron/exon boundary that is conserved in both MIP and Fulicin. B) Transcriptional profile showing the number of sequences in our 454 library that align to the unique region of TxFrag2, MIP, and Fulicin in the sense and antisense direction. TxFrag2 is present mostly in the antisense direction, suggesting it may function in a regulatory mechanism.

454 Counts (1,500,000 total)

Sense AntisenseTxFrag2 6 50MIP 9,810 325Fulicin 1 0

65

CHAPTER 4 GENE EXPRESSION PROFILING FOR INDIVIDUAL NEURONS

Introduction

The primary motivations for the identification of putative neurosecretory products as

described here was to identify neuron-specific signaling molecules that may be responsible for

neurona l identity, communication and p lasticity. While other labs have created comprehensive

lists of putative secreted signaling molecules in other models, the complexity of the brain

systems in these models has presented a challenge to identifying neuron-specific neurosecretory

products. Our lab has combined high throughput sequencing with the large and easily

identifiable neurons of Aplysia californica, to determine what signa ling molecules are expressed

in individual identified neurons.

Advantages of Aplysia

In order to pe rform gene expression profiling o f a single neuronal type, a homogenous

sample of RNA must be isolated from an individual neuron. In many model systems this would

be difficult if not impossible due to the complexity of the brain tissue, the inability to identify

and isolate single neurons, and the small size of the cells. However, the large and identifiable

neurons of Aplysia offer a means of accomplishing this goal.

The gill and siphon withdraw of Aplysia represents a simple and quantifiable behavior with

a well defined network of neurons. The cellular circuitry that drives this behavior can be

simplified to two neurons of the abdominal ganglia, a sensory cell (SN) and a motor neuron (L7).

Due to the location of these cells, a semi intact preparation can be used to confirm the identity of

these cells using e lectrophysiology (Figure 4-1). By using this type of arrangement our lab has

identified ind ividual motor , sensory and interneuron cells that can be used to provide insight into

the expression of transcripts in a single neuron type.

66

In addition to providing the ability to identify and isolate individual neurons, the giant

polyploidy neurons of Aplysia contain vast amounts of genetic material with a chromosome copy

number up to 100,000n (Gillette, 1991) and RNA content estimated at up to 0.2 µg in some

cases. This makes Aplysia an attractive model for gene expression profiling as large amounts of

RNA can be made readily available for sequencing.

Limitations of Sequencing Technology

Over the last several decades, our understanding and application of high- and low-

throughput methods to study gene expression has continued to grow. Each method has different

sensitivities as well as advantages and disadvantages due to basic technological constraints and

the absolute number of each mRNA molecule to be measured. One major obstacle to using

high-throughput sequencing to measure gene expression is the amount of data generated by this

method. For research scientists, the complexity of the transcriptome becomes overwhelming, if

not impossible, to understand without the use of bioinformatics approaches.

As the sequencing technology continues to grow, and EST coverage begins to reach

genomic scales, many researchers are faced with a daunting task of sifting through generated

data to find transcripts with function relevant to their research aims. To address this problem,

our lab has devised a method for quantifying transcript expression and abundance, in a user

friendly output, to create a digital expression profile (DEP) for each transcript. We have applied

this method to individual neurons in the memory-forming network of Aplysia to determine what

transcripts are relevant for the function and maintenance of specific cell types, including motor

neurons (MN) L7 and R2, sensory neurons (SN) and MCC interneurons.

Here, we applied the method to a selected list of transcripts previously predicted by our lab

to encode secreted signa ling molecules to quickly screen hundreds of transcripts from individual

67

cells invo lved in learning and memory in Aplysia. This method will be particularly useful for

studying model systems that are lacking in genomic information

Results and Discussion

From the DEP, preliminary analyses of selected transcripts encoding secreted signa ling

molecules could be easily screened to de termine differential expression in specific neuron types

(i.e. motor (L7), sensory (SN cluster) and interneurons (MCC)). It also allows us to identify

abundant transcripts for each neuron type as indicated below.

Sensory Neurons

The digital expression profile using neurosecretory products predicted by traditional

cloning for sensory neurons supports previous work indicating robust expression of Sensor in A

in sensory neurons (Cai et al., 2008) (Figure 4-2). As expected from previous publications,

Sensorin A appears to be the most abundant transcript encoding a secreted signaling molecule in

the sensory cell. In add ition to Sensor in A, DEP indicates a low expression of other

neurosecretory transcripts determined by traditional cloning including Prothoraracicostatic

peptide - like protein (PTSP) and Capsulin (Figure 4-2). Further profiling using SSMs identified

here (Figure 4-3) suggests that sensory cells of the gill and s iphon withdraw response may also

express low levels of the predicted SSMs, LFRFamide precursor and SFY3-like peptide. DEP

also suggests expression of predicted 7B2 secretory granule neuroendocrine protein which is

unlikely to a secreted signaling molecule but may be involved in the processing of

neuropeptides. Screening of the SSMs predicted from the Lottia genome shows low expression

of heatshock, and an unknown protein product (Figure 4-4).

Motor Neurons

Our digital expression profile of neurosecretory products predicted by traditional cloning

(Figure 4-2) and homology search (Figure 4-3) support previous experiments suggesting both

68

MIP (Figure 4-3) and FMRFamide (Figure 4-2) are expressed in the L7 motor neuron.

Furthermore, these DEPs and that for the neurosecretory products predicted using a genomic

approach suggest the expression of several other putative neurosecretory products including

Capsulin, R15-1 and R15-2, Delta- like precursor, Ependymin related protein, 7B2 secretory

granduale neuroendocrine protein, LFRFamide, Putative Phermone-2, Theromacin like,

heatshock- like protein, and Fulicin. However, further investigation with in situ hybridization

does not support the expression of most of the products predicted by DEP including Capsulin,

R15-1 and R15-2, and Delta- like precursor. While some of these predicted SSMs may not be

involved in cell signaling, several, including Fulicin, LFRFamide, and Ependymin-related

protein-2 may prove to be key signaling molecules involved in cell-to-cell communication

including retrograde signaling.

Of particular interest to this thesis was the prediction of a Fulicin- like neurosecretory

product in Aplysia and DEP suppor ting the expression of Fulicin in L7 motor neuron (Figure 4-

4) as suggested in Chapter 3 of this thesis.

Interneurons

DEP reveals the expression of Capsulin, FMRFamide, Myomodulin, R15-1 and R15-2,

7B2 secretory granduale neuroendocrine protein, Insulin- like proteins, LFRFamide, putative

Phermone-2, Buccalin, and heatshock protein in MCC interneurons. In situ hybridization has not

confirmed the expression of any MCC specific neurosecretory product, suggesting that all

proteins predicted to be expressed in MCC by DEP are due to inputs from synaptic terminals of

other neurons.

69

Discussion

Digital Expression Profling

Although some transcripts are co-expressed in different neuron types, the level of

expression for each type is unique. Furthermore, the overall expression profile of transcripts

across neuron types is unique, creating a sort of laundry list of transcripts unique to cell function.

This presents a potential method for identifying neuron function based exclusively on molecular

data and excluding the need for physiological studies.

Furthermore, this information can be applied to systems like the gill and siphon withdraw

network in Aplysia californica to enhance our understanding of key molecular components of a

learning network. We also suspect that transcripts with a low frequency of expression in DEP

may indicate contamination to the library by way of synaptic input from other neurons in the

circuit. Indeed, by combining DEP with in situ hybridization, we have found some transcripts

with low DEP expression do not show any positive labeling using in situ hybridization methods

(Table. 4-1). While this may at first seem a drawback to the sequencing technology, this

contamination could actually be used as insight to the identification of synaptic input to specific

neurons being studied.

Implications of Neuron-specific Expression

While the cellular mechanics underlying learning and memory of the gill and siphon

withdraw network has been well defined and studied, limitations in the molecular understanding

of this network has hindered progress in understanding cellular identity and plasticity. Some

signaling molecules of this system have been identified (i.e. Sensorin) while others, for example

the molecules are involved in the retrograde signaling from L7 neurons to sensory neurons,

remain elusive. This work presents a major contribution to the understanding of the molecular

mechanisms underlying cell identity and plasticity in a learning behavior. From here it is hoped

70

that researchers will be able to study the effects of identified putative neurosecretory products to

determine what if any role these molecules have on cell signa ling and learning and memory. It is

likely that several of these identified putative neurosecretory products may prove to be crucial to

the overall understanding o f this neuronal circuit.

Methods

Animals and Dissection

Aplysia californica weighing up to 150g were obtained from the National Resource for

Aplysia at the University of Miami, and Aplysia weighing 150g-400g were collected in the wild

by Marinus Scientific, Long Beach, California. Animals were anesthetized by injection of

isotonic (340 mM) MgCl2

Single neuron collection was performed previously by Thomas Ha. Briefly, ganglia were

first incubated in 1% Protease IX (Sigma) at 34º for 45 min to soften the connective tissue of the

neuronal sheath. Then ganglia were pinned to a sylgard dish in artificial seawater (ASW: 460

mM NaCl, 10nM KCl, 55mM MgCl

(approximately 50% of the body weight) before removal of muscle or

nervous tissue.

2, 11 mM CaCl2,

Construction of 454 Libraries

10mM HEPES, pH 7.6), the cells were

exposed by mechanical removal of overlying sheath with fine forceps, and the dish was flooded

with 70% ethanol. After two minutes the cells were mechanically removed with fine forceps

placed in 250 µl 70% Ethanol and stored at -20C until RNA isolation. The MCC neurons were

identified visually. The identity of the L7 neurons was first confirmed by electrophysiology (see

Figure 4-1) before fixation and collection.

Libraries were constructed as previously described in this thesis, Chapter 2 methods.

71

Digital Expression Profiling

Digital Expression Profiles (DEP) are made by taking the entire set of data from a

sequencing project before assembly. In this dataset, each sequence read represents the

expression of a single transcript in the cell or tissue the library was made from, such that there is

a 1 to 1 ratio of sequence read to transcript.

From this data set, any sequence of interest can be aligned using BLAST (1e-04) and by

counting the number of reads generated from the 454 sequencing that have significant BLAST,

the number of times that transcript was sequenced from the library can be determined. To

determine the frequency of expression, these counts are normalized to the total number of

sequences in the sequencing pr oject. By normalizing the counts, the frequency of expression

across different sequencing projects can be compared.

72

Abdominal Ganglia

SiphonGill

Figure 4-1. Semi- intact preparation of Aplysia abdominal for identifying neurons of the gill and siphon withdraw reflex. The nerves connecting the abdo mina l ganglia to the gill and s iphon remain intact. By removing the sheath surrounding the neurons, cells can be impaled and recorded from to determine cell identity. For example, L7 can be identified by impaling cells in the vicinity of L7 until a cell is found that when stimulated, results in the contraction of the gill and s iphon.

73

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

0

0.001

0.002

0.003

0.004

0.005

0.006Fr

eque

ncy

of E

xpre

ssio

n

Neurosecretory Protein

L7 FrequencySN FrequencyMCC Frequency

capsulin FMRFamide

myomodulin

PTSP-like peptide

R15-2

R15-1

Sensorin-A

Figure 4-2. Digital expression of neurosecretory products predicted by traditiona l cloning.

74

Figure 4-3. Digital expression of neurosecretory products predicted by homology search against unbiased shot-gun sequencing.

Predicted Neurosecretory Products

Freq

uenc

y of

Exp

ress

ion

7B2 secretory granule neuroendorine protein

Delta-like precursor

Ependymin-related protein-2

Insulin-like

LFRFamide precursor

MIP

Putative Pheromone-2

SFY3-like peptide Theromacin

75

Figure 4-4. Digital expression of neurosecretory products predicted by genomic approach.

Reticulocalbin1 precursor

Kinesin-like

Insulin-like

Unknown

Unknown

Unknown

hypothetical protein

Buccalin

Unknown

FlbAproteinBEL-2

Unknown

UnknownFulicin

ependymin-related

VonWillebrand factor precursor

Unknown

similar to orphan G protein

Cell wall protein DAN4 precursor

P protein

Unknown

Unknown

Out at first protein

hypothetical protein

Heatshock

Unknown

unknown

IG-H3 precursor

0

0.00005

0.0001

0.00015

0.0002

0.00025

0.0003

0.00035

0.0004

0.00045

0.0005

Freq

uenc

y of

Exp

ress

ion

Predicted Neurosecretory Product

L7 FrequencySN FrequencyMCC Frequency

76

Secretory Product DEP In situ

SN MN IN SN MN IN

Sensorin + + +

PTSP-like + +

Capsulin + + +

LFRFamide + + +

SFY3 +

7B2 s.g. + + +

Heatshock + + +

MIP + +

FMRFamide + + +

R15 + +

Delta-like +

Ependymin + +

Putative Pheremone 2 + +

Theromacin +

Fulicin +

Myomodulin + +

Insulin-like + +

Buccalin + + +

Table 4-1. Validation of DEP using in situ hybridization. Comparison of secretory products predicted to expressed in Sensory (SN), Motor (MN) or Inter (IN) neurons by DEP compared to expression confirmed by in situ hybridization.

77

Object 2-1. Intron/Exon Boundaries of selected neurosecretory genes.

Preliminary analysis of neuropeptides across gastropod species Aplysia and Lottia suggests

that some neuropeptides evolve at different rates. These results (see Chapter 2) suggest that

some neuropeptides involved in development (i.e. Dorsal-ventral patterning peptide) have a more

closely conserved coding sequence than those neuropeptides involved in neuron regulation (i.e.

MIP). To see if these differences are due to underlying discrepancies in the neuropeptide

genomic organization, the intron/exon boundaries of 12 selected neuropeptides were examined.

Selected neuropeptides show a relatively simple genomic organization, with an average of

3-5 exons. Those neuropeptides shown to be more highly divergent do not appear to have

differences in their genomic organization compared to closely conserved neuropeptides.

Atrial gland-specific antigen precursor (AGSA) (Mollusk-derived growth factor) (MDGF) Gene Bank Accession Number: 22261794 Intron /Exon Boundaries :

Exon Number

Intron 3’ Sequence

Exon 5’ Sequence Exon 3’ Sequence Exon Length


1 gggcaggt ag CCATGTCGTC CATCTTGGCG 209 gt acgtttaa 2 tcttgttt ag GAAAAAAGAA ATGCCTAAAG 130 gt aagggatt 3 ctccccccc ag GCGGTGCTCT CCGGACCCAT 147 gt gagtctat 4 tcattccc ag TTTGTGTTTG TCGATGACTG 67 gt aaggaggc 5 tgctgtac ag GTTGAAGAAA TTTTGCTCAG 97 gt aagtgaaa 6 ttatcatc ag GTCATCGGAC GCTGTTTGGG 114 gt gagtcaaa 7 acccctgc ag TTCTTCGAGC CTGGCCTAAG 128 gt aggctaaa 8 ttccctcc ag GTTCAAGAGC GCTGAAACCA 206 gt acgtatgc 9 ctgttaac ag ACTGGCAGGA CTCTAATCAG 158 gt ttgtagag 10 tgtcctgc ag ATCCTGGGCC CTATCCCATC 69 gt tatctcct

Intron 1 2 3 4 5 6 7 8 9

78

Feeding circuit activating peptide precursor Gene Bank Accession Number: 22947345 Intron/Exon Boundaries: Exon Number



Intron 5’ Sequence Placement in Sequence

1 GACCAGACAG CCTCAACTTC CCACTGCAAG 87 GTAAGAAAAC -18-69 2 TCTCGTTCAG ACACCGGAAC AAATGTAAGG 2162 GTCAGTTACA 70-2231

FMRFamide neuropeptide precursor Gene Bank Accession Number: 84551 Intron/Exon Boundaries: Coding Region: 149-1942 nt Exon Number




1 tcaaatct ag AGTTCTTCAA CCGACTGATC 137 gt aagttgct -20-117 2 caatctgc ag GCGCCCGTGA CTGTGCGAGA 140 gt gagtaccc 118-257 3 ggtatctg ag ATTTGGCCGG AATACAACCA 1695 gt gatatttg 258-1952

L11 neuropeptide precursor Gene Bank Accession Number: 155779 Intron/Exon Boundaries : Exon Number




1 ggaccccc ag GTCATCATGC CTGCACGTGA 117 gt tgtcgttg -6-110 2 cccacagg ag GTTTGTTTTCG CCTACAACAG 141 gt gggctata 111-251 3 ttttccac ag AGTTTTCTTA ACGAAACTAT 87 gt caatagac 385-471

79

Buccal in precursor Gene Bank Accession Number: 404497 Intron/Exon Boundaries: Coding region: nt 271-1786 Exon Number



Intron 5’ Sequence Exon on Seq

1 tttcggca ag AAATCCTGAC ACACACGCAG 181 gt agggatctg 1-167 2 tttgtttc ag TTTACTTAAC GCCTTTATCT 86 gt aagacttg 168-253 3 cctccccc ag GTTCCCCCCC AGAGGAACAG 1516 gt gagctggc 254-1770 4 ctccctcc ag TCGTCGAAGA AGTGACCGCT 29 gt gacgtagt 1771-1800

ConopressinGene Bank Accession Number: FJ172359

*

Intron/Exon Boundaries: Coding region: nt 106-684 Exon Number



Intron 5’ Sequence Exon on Seq

1 gtttttaa ag TTTGCATAAG TTAGAGGCAA 58 gt ttcaagtg 19-72 2 tcctgcac ag AACGAAATCA ACAGCGACAG 195 gt aagaacgt 73-267 3 ttatttcc ag TGCATGGCGT TGTGATTCAG 208 gt aggtcgtc 268-475 4 ttcccccc ag AATCATGTGC GCCCAGTGAC 209/1516 gt gaggagcc 476-684

Fulicin-like neuropeptide precursor+

Gene Bank Accession Number: 56792350

Intron/Exon Boundaries: 353-1533 Exon Number



Intron 5’ Sequence Nucleotide location

1 GGGGATCATC TGACCATAGA - gt atgttgtt 1-247 2 gttgtttc ag ATTCCAAAAA AGTGGTTCAG 144 gt aggttgct 248-391 3 tctcgttt ag GTTACCACAG AATTGTACTG 33 gt gagtggaa 392-424 4 cttattcc ag ATTTTTTCTG TCACGTGCAG 107 gt gagtagga 425-531 5 ctccatcc ag ATACCACGAA ATAGGACACG 1001 gt attttcta 532-1533

80

MIP-related peptide precursor Gene Bank Accession Number: 8886135 Intron/Exon Boundaries: coding region: 668-2873 nt Exon Number




1 CCCTTGGGGT TGACCATAGA - gt atgttgtt 1-456 2 gttgtttc ag ATTCCAAAAA AGTGGTTCAG 144 gt aggttgct 457-600 3 tctcgttt ag GTTACCACAG AATTGTACTG 33 gt gagtggaa 601-633 4 cttattcc ag ATTTTTTCTG TCACGTGCAG 107 gt gagtagga 634-740 5 ttccatgc ag ATACGCAAAG AGGTAGCTTT 2139 gt ccatccag 741-2879

Neurotoxin-like-1Gene Bank Accession Number: 71148939

+

Intron/Exon Boundaries: Coding Region: 10-423 nt Exon Number




1 GCTGAGCA AG TCACAATCAC CAGCGTGAAC 70 GT AAGTCTTA -10-60 2 TCCTCCCC AG GCAAAGGTGG GCAACGAAAG 130 GT GAGGCGTG 61-190 3 GATATTGC AG GGACGTGCCA TTCGTTCCAG 129 GT CAGTACAC 191-319 4 CTTACGTC AG GATTCTTTTC CATAGATGAC 129 GT CAACACCT 320-448

PleurinGene Bank Accession Number: 56200040

+

Intron/Exon Boundaries: Coding Region: 189-755 Exon Number




1 TTGTCACCAG AAGTGAGTGA CTCACCAACA 155 GTAAGCCATC 2-156 2 TATCAAACAG TAAACCGCTC CTTGAGCTCA 482 GTGGCCGAAT 176-657 3 TGTTTCACAG GTGATCAAGC CTTTGATGAC 94 GTCATCTGAG 682-776

81

Whitnin precursor (SPTR)Gene Bank Accession Number: 56200044

+

Intron/Exon Boundaries: Exon Number



Intron 5’ Sequence Placement in S Sequence

1 TGTGTTTCAG ATCCTTCAGC TGTGCTGCAG 115 GTCAGACACA -13-102 2 TGTCTTCCAG GAGGCCTCGG CATTCAAGAG 99 GTGGGTGGCA 103-201 3 ATCATCTCAG CTTGTGGACG GGAGTGGTAC 146 GTGCCTTGGG 202-347

82

Object 2-2. Identified neurosecretory products likely to be signaling molecules.

Secreted Signal Molecules found prior by traditional cloning

Abdominal ganglion neuropeptides L5-67 p recursor Gene Bank Accession Number: 113521 Reference: Shyamala, M., Fisher, J.M. et al. (1986). A neuropeptide precursor expressed in Aplysia neuron L5. DNA. 5(3), 203-8. Aplysianin A precursor Gene Bank Accession Number: 26419361 Reference: Cummins, S. F., Nichols, A. E. et al. (2004). Characterization of Aplysia enticin and temptin, two novel water-borne protein pheromones that act in concert with attractin to stimulate mate attraction. J Biol Chem. 279(24), 25614-22. Atrial gland-specific antigen precursor (AGSA) (Mollusk-derived growth factor) (MDGF) Gene Bank Accession Number: 22261794 Reference: Sossin, W. S., Kreiner, T. et al. (1989). A dense core vesicle protein is restricted to the cortex of granules in the exocrine atrial gland of Aplysia california. J Biol Chem. 264(28), 16933-40. Attrac tin precursor Gene Bank Accession Number: 38257306 Reference: Fan, X., Wu, B. et al. (1997). Molecular cloning of a cDNA encoding a potential water-borne pheromonal attractant released during Aplysia egg laying. Brain Res Mol Brain Res. 48(1), 167-70. Calreticulin Gene Bank Accession Number: 262054 Reference: Kennedy, T. E., Kuhl, D. et al. (1992). Long-term sensitization training in Aplysia leads to an increase in calreticulin, a major presynaptic calcium-binding protein. Neuron. 9(6), 1013-24. Capsulin Gene Bank Accession Number: 31088940 Reference: Cummins, S. F., Nichols, A. E. et al. (2004). Characterization of Aplysia enticin and temptin, two novel water-borne protein pheromones that act in concert with attractin to stimulate mate attraction. J Biol Chem. 279(24), 25614-22. Cerebrin prohormone precursor Gene Bank Accession Number: 74843746 Reference:

83

Li, L., Floyd, P. D.et al. (2001). Cerebrin prohormone processing, distribution and action in Aplysia californica. J Neurochem. 77(6), 1569-80. Clionin precursorGene Bank Accession Number: FJ214338

+

PubMed ID Reference: n/a not released CDS: ATGACGTCATCCATGATCCTCGCTGTCCTGGCCATCTCCCTGTCGTGCCTGCTGACCGCTGTGACGTCATCACCACTTGGCCCAGTCCAGCCGGCCATATCCCTGTCCAAGATGGAACCCGATCACGCCTGTTTGTTCATGTGCAACATCTGTTTCCCGGATCTGGAGGACACGGGTCTGCTCCTGGACTGCAGCAACAAAGTGTGTGGTCCCATCATGGCGGGCATGTGTGCTATGGAGAAGCTCGTCTGGCTGGGCCACAACTGCCGTCAGTACGACATGGTGCAGAAAATGTGGGCTCCGCACGGCGCTCTCTAA Protein: MTSSMILAVLAISLSCLLTAVTSSPLGPVQPAISLSKMEPDHACLFMCNICFPDLEDTGLLLDCSNKVCGPIMAGMCAMEKLVW LGHNCRQYDMVQKMWAPHGAL ELH precursor [Contains: Beta-bag cell peptide (Beta-BCP) Gene Bank Accession Number: 119268 Reference: Scheller, R. H., Jackson, J. F. et al. (1983). A single gene encodes multiple neuropeptides mediating a stereotyped behavior. Cell. 32(1), 7-22. Enticin precursor Gene Bank Accession Number: 74814556 Reference: Cummins, S. F., Nichols, A. E. et al. (2004). Characterization of Aplysia enticin and temptin, two novel water-borne protein pheromones that act in concert with attractin to stimulate mate attraction. J Biol Chem. 279(24), 25614-22. Feeding circuit activating peptide precursor Gene Bank Accession Number: 22947345 Reference: Sweedler, J. V., Li, L. et al. (2002). Identification and characterization of the feeding circuit activating peptides, a novel neuropeptide family of Aplysia. J Neurosci. 22(17), 7797-808. FMRFamide neuropeptide precursor Gene Bank Accession Number: 84551 Reference: Taussig, R. and Scheller, R. H. (1986). The Aplysia FMRFamide gene encodes sequences related to mammalian brain peptides. DNA. 5(6): 453-61. FUR Gene Bank Accession Number: 453657 Reference: Chun, J. Y., Korner, J. et al. (1994). The function and differential sorting of a family of Aplysia prohormone processing enzymes. Neuron. 12(4), 831-44. Glycoprotein hormone beta subunit (GPB5) +

84

Gene Bank Accession Number: AY928334 Reference: Heyland, A., Moroz, L. (2005). Unpublished. Hypothetical protein Gene Bank Accession Number: 26892018 Reference: Cummins, S. F., Nichols, A. E. et al. (2004). Characterization of Aplysia enticin and temptin, two novel water-borne protein pheromones that act in concert with attractin to stimulate mate attraction. J Biol Chem. 279(24), 25614-22. Insulin precursor Gene Bank Accession Number: 8886137 Reference: Floyd, P. D., Li, L. et al. (1999). Insulin prohormone processing, distribution, and relation to metabolism in Aplysia californica. J Neurosci. 19(18), 7732-41. L11 neuropeptide precursor Gene Bank Accession Number: 155779 Reference: Taussig, R., Kaldany, R.R. et al. (1984). A cDNA clone encoding neuropeptides isolated from Aplysia neuron L11. Proc Natl Acad Sci U S A. 81(15), 4988-92. Myomodulin [Aplysia californica] Gene Bank Accession Numbe r: 400327 Reference: Lopez, V., Wickham, L. et al. (1993). Molecular cloning of myomodulin cDNA, a neuropeptide precursor gene expressed in neuron L10 of Aplysia californica. DNA Cell Biol. 12(1): 53-61. Myomodulin gene 2 neuropeptide precursor Gene Bank Accession Number: 77378085 Reference: Proekt, A., Vilim, F.S. et al. (2005). Identification of a new neuropeptide precursor reveals a novel source of extrinsic modulation in the feeding system of Aplysia. J Neurosci. 25(42), 9637-48. Neuropeptide precursor 1 Gene Bank Accession Number: 20372612 Reference: Matz, M.V., Meleshkevitch, E. et al. (2001). Unpublished Neuroprepeptide Gene Bank Accession Number: 155771 Reference: Nambu, J. R., Taussig, R. et al. (1983). Gene isolation with cDNA probes from identified Aplysia neurons: neuropeptide modulators of cardiovascular physiology. Cell 35(1), 47-56. PRQFVamide precursor protein

85

Gene Bank Accession Number: 29469911 Reference: Furukawa, Y., Nakamaru, K. et al. (2003). PRQFVamide, a novel pentapeptide identified from the CNS and gut of Aplysia. J Neurophysiol. 89(6), 3114-27. PTSP-like peptide neurotransmitter precursor+


Reference: Moroz, L. L., Edwards, J. R. et al. (2006). Neuronal transcriptome of Aplysia: neuronal compartments and circuitry. Cell 127(7), 1453-67. Putative pheromone Gene Bank Accession Number: 115371642 Reference: Cummins, S. F., Degnan, B. M. et al. (2008). Characterization of Aplysia Alb-1, a candidate water-borne protein pheromone released during egg laying. Peptides 29(2), 152-61. R15-1 neuroactive peptide precursor Gene Bank Accession Number: 155797 Reference: Buck, L. B., Bigelow, J. M. et al. (1987 ). Alternative splicing in individual Aplysia neurons generates neuropeptide diversity. Cell 51(1), 127-33. R15-2 Neuroactive polyprotein precursor Gene Bank Accession Number: 113523 Reference: Buck, L. B., Bigelow, J. M. et al. (1987). Alternative splicing in individual Aplysia neurons generates neuropeptide diversity. Cell 51(1), 127-33. R3-14 neuropeptide precursor Gene Bank Accession Number: 155782 Reference: Scheller, R. H., Kaldany, R. R. et al. (1984). Neuropeptides: mediators of behavior in Aplysia. Science 225(4668), 1300-8. Sensorin-A precursor Gene Bank Accession Number: 134428 Reference: Brunet, J. F., Shapiro, E. et al. (1991). Identification of a peptide specific for Aplysia sensory neurons by PCR-based differential screening. Science 252(5007), 856-9. Temptin precursor Gene Bank Accession Number: 74811743 Reference: Cummins, S. F., Nichols, A. E. et al. (2004). Characterization of Aplysia enticin and temptin, two novel water-borne protein pheromones that act in concert with attractin to stimulate mate

86

attraction. J Biol Chem. 279(24), 25614-22. Tolloid2Gene Bank Accession Number: AY485265

+

Reference: Brown, B.D., Kohn, A.B. et al. (2003). Unpublished. CDS: GCGCGCCATGGAGAACACAGTGCGAAAAGTTGCTCCTGTTCGAACTGCTAACAGAATCATCAATCTGTTTTGCTTGTGTACTTGTGCCTTGGTGCTGCAGTGTGTTTTGTTCCCCGCCACTGCCGAAAACATTCCAAGACGAACGCCCGACGACCTGTACATGAATCCATGCAAAGCTGCTGGATACTTGGATGGTATCGCTCTAACTGAAAACGAATACGAATCGTTTGAGAGGAGGTGGCTGGCTCTCAAGAACAACATTTCCGATAGTCCTCAACAGTCAATTGTCAACCATACAGACGCAGATAGGACGCAGTCACCCAGGGCTGTTACAGGTGACGCCATGGATATGGAGAAGAGAAAACTTCGTCTACAGAGTTTGGTGGAGGAGAAAGACGAACTCACGCAGTTAATCAAAGAAGCGCGTGCAGAGCTGAAAAAAACTTGCCATGACAGGTCAGACAGCGAATGTGACCAGGAGAAAGAAACGCTTCACAAGCTACTGTCGGAGGCGAGACGCAAAAGAAAACAGATAAAGCTCAGACTGCATCGACTTCGGAAAGAGATTGTTCAGGGAGATGAGAACGAAAGTGACGGACGTCCTCACTCCTCCTCTTCTCGGGGGAGACACAGACGTGCGACACCTGCTGATAGGTCAAAGTTGTGGGACCATGGCGTTATCCCTTATGTCATTGAATCAAATTACTCGGGTGAGAACAAGGACCTGTTCAAGCTGGCCATGAGACACTGGGAAAACCTCACGTGCCTCGTGTTCAAGGATAAAGGTCCGGAGGATACGAATTATATTCTTTTTACCCAGACGGATTGTGGATGTTGCTCGTTCGTTGGGAAGCACGGGAGTGGAGCCCAGGTGATTTCTTTGGGCAAAGGCTGCTACTATTTCGGCACCGTCGTGCACGAGCTGGGTCACGTGGTCGGCTTCTGGCATGAGCACAACAGGCCGGACAGGGATAAATACGTACAGATAATACGGAAGAACATTATGCCAGGCAAAGAAAGCGAGTTCAACATCTTGGACGAAGATAAAGTGGATTCCCTTGGTGAACCGTACGACTATGGCAGCATCATGCACTACTCTCGTGACAAGTTCTCCAAGCACAGCTACCTGGACACCATCCGGCCGTTCCGTCAGAGAGGCATGATCGCCCTGCCCCGCATCGGCCAAAACATACGACTCAGTGATGGGGACGTACGACAGACCAACAAACTCTACAAGTGCCCTACTTGTGGCCGCACCGTGATGGAATCAAAGGGCACGATTAGCCCCGACTCAGGGATACGAGGCGAAAGGACGTGCCAATGGAGGATCATAGCCTCTCACGGGGAGCGGATCCAGCTGAGCCTGACCCGACTTGACCTCAGCAACTGTGACACAGACTACGTGGAGGTCAGGGATGGACATTTTGTCGGCTCTCTCAGTTTGGGCAAATTCTGTGGGAAGAAGATCCCCCCGCCCATGATCTCCTCCGGCACTCGCCTCTGGGTGGAGTACAAGTCAAGGGCGGCGCGCCCGGGAGCTTTCCAGGCCGCTTTTGAGGCTATATGCGGTGGTAACATGCCTGGACCCGAAGGCTTCCTCAACAGTCCCGCTTATCCGGATGAGTATGGCAGTGATAAAGTCTGCGAGTGGGTCATCACTGTGAGAGAGGGATATCAAGTGGCTCTAGAGTTCGCCACTTTCGAGACGGAATTCGACCCTGACTGTGCCTACGACTACGTAGAGATCCGAGATGGTGACACAAAAGACTCGCCTCTTGTGGGTACATATTACGGGGGTTACTTGGACGCACCCAGTGGGACCATCTCCTCCCCGTCCTTCCCTGACCTCTACCCCCCAGACAAGAACTGTGTCTGGCACATCAGCGCGCCCAAGGGACACACCCTCACCGTGAACTTTACTCATATGGATCTGGA GTGGAGGGGGGATGAATGTGAATTAGACTTCGTGCGTGTGACAAACGTCGTTGGTAATAAGGAAAGACTACAAGGCCAGTACTGTGGCTTCATGGCCCCGCCCTCAATTACGTCACTTAGCAACGAGCTGAGAATAGAATTCCGGTCGGATGACACTTTGCAAAAGACAGGATTCTCTATGGACTATGTGGCAGACGTGGACGAGTGTGCCAGCAGCAATGGCGGCTGCAAGCATATCTGTGAGAACACTGTGGGAAGCTTCCGCTGTTCGTGTCGTGAGGGTTTCATCCTGGCCGATGACGAGAAGAGCTGCAAGGAGGGCGGCTGCCATTACGAGGTGACAGATACCAAGGGCGTCATCCAGAGCCCAGATTACCCAAGCTTCTACCCGGCCAGGCGAGACTGTGAGTGGCATTTCACCACAGCACCAGGACACGCTGTCCGTCTCATATTCACGGATTTCCAGGTTGAGCCTCACAGGACTTGTAGGTACGATCACGTAGAGGCGTTTGACGGTGCAAATATTCAGGCACCACAGATTGGCAAATACTGCGGCTCAGAAAAGCCTGCACCAATCATATCTTCCGAAAACACGTTGACCTTGACCTTCCTCTCTGACACGTCTGTGCAGCGGAAAGGATTTAGAGCCCGTCATGACACTGTGTGTCAGTCGTCACCCACAGCTACGAGTGCCCCCAAGAAGATTCTGTCCCACGTCCTGTACGGCAGCAAACCCTACGACAACAGACAGAACTGCTCCTGGAACATCCAGGCGCCCGAGGGCCAGCACGTGGAGCTCAGGTTCACAGCCTTTGAGATTGAACAACAGTCCAGATGCCTGTACGACTACGTGGCTGTCTACGATGGGCCGACGGAGAATGACCTGGTCCTCGGCAAGTTCTGTGGGAATCAGGTCCCAAGCCCTATCGTCTCCAGTACACGCAGTCTTCTCGTTCGCTTCCGCTCTGACGATACGATCAAATCTGGAGGCTTCAGCGCCACCTATCGCATTGCAGATGACACGGACCACACAGTGGATCTGCTGAGCCAGTGAAAACAAAAAGGAGCAACGCCTACGGGGTTCAACAATTGATGAAATGTTACTGATTATATTGAAAGCAAATATGCAGTTTTCATGTTGAACTCTGGTAAATGAAAAGTCTTCCCCCCAGCAAGACTGTACTGAACTGTTATCTATCGCCTATTATTATCACCATCCTAACAAGATCATGTATAATAATCAAATCAGAGTCTCTTAATATTAGGCCTACGTTACCCATATTGCACACGACCGACTTAGAGACCAGTAAATCCTGCAATTGTA

87

CAAACACATGTATGTGGGTTCTGCAACAGTAGCCCCCCTTTTCCACGCACAGAAAGGGGAGGCGTCTGCGAAGTCAACAGGAACGGATGTGAAGCAT Protein: MENTVRKVAPVRTANRIINLFCLCTCALVLQCVLFPATAENIPRRTPDDLYMNPCKAAGYLDGIALTENEYESFERRW LALKNNISDSPQQSIVNHTDADRTQSPRAVTGDAMDMEKRKLRLQSLVEEKDELTQLIKEARAELKKTCHDRSDSECDQEKETLHKLLSEARRKRKQIKLRLHRLRKEIVQGDENESDGRPHSSSSRGRHRRATPADRSKLW DHGVIPYVIESNYSGENKDLFKLAMRHW ENLTCLVFKDKGPEDTNYILFTQTDCGCCSFVGKHGSGAQVISLGKGCYYFGTVVHELGHVVGFW HEHNRPDRDKYVQIIRKNIMPGKESEFNILDEDKVDSLGEPYDYGSIMHYSRDKFSKHSYLDTIRPFRQRGMIALPRIGQNIRLSDGDVRQTNKLYKCPTCGRTVMESKGTISPDSGIRGERTCQWRIIASHGERIQLSLTRLDLSNCDTDYVEVRDGHFVGSLSLGKFCGKKIPPPMISSGTRLWVEYKSRAARPGAFQAAFEAICGGNMPGPEGFLNSPAYPDEYGSDKVCEW VITVREGYQVALEFATFETEFDPDCAYDYVEIRDGDTKDSPLVGTYYGGYLDAPSGTISSPSFPDLYPPDKNCVWHISAPKGHTLTVNFTHMDLEW RGDECELDFVRVTNVVGNKERLQGQYCGFMAPPSITSLSNELRIEFRSDDTLQKTGFSMDYVADVDECASSNGGCKHICENTVGSFRCSCREGFILADDEKSCKEGGCHYEVTDTKGVIQSPDYPSFYPARRDCEWHFTTAPGHAVRLIFTDFQVEPHRTCRYDHVEAFDGANIQAPQIGKYCGSEKPAPIISSENTLTLTFLSDTSVQRKGFRARHDTVCQSSPTATSAPKKILSHVLYGSKPYDNRQNCSWNIQAPEGQHVELRFTAFEIEQQSRCLYDYVAVYDGPTENDLVLGKFCGNQVPSPIVSSTRSLLVRFRSDDTIKSGGFSATYRIADDTDHTVDLLSQ& Small cardioactive peptides precursor Gene Bank Accession Number: 134314 Reference : Sossin, W.S., Kreiner, T. et al. (1989). A dense core vesicle protein is restrictued to the cortex of Granules in the exocrine atrial gland of Aplysia californica. J Biol Chem. 264(28), 16933-40.

Transcripts predicted by homology s earch to encode secreted proteins

Achatin-like neuropeptide precursor+


Reference: Moroz, L. L., Edwards, J. R. et al. (2006). Neuronal transcriptome of Aplysia: neuronal compartments and circuitry. Cell. 127(7), 1453-67. CDS: ATGACGTCATCTGCTCAATGCCTTCTGCTCACAATGCTCCTAGTGGCCACCCTTGACTTCCTGTACGCAGAAGACATCGGCTTCGCGGAAACAGACTCGGAACTGACGCCCTTTGCCGGGGCAGTCGCCGACCACGTCCTGAAAGAGGGCTTCGGGAAGAGGGGCTACTTCGACAAGCGGGGCTTTTTCGACAAACGAGGTGACGCAAGCAAACGCGGCTTCTTCGACAAGCGAGGGTTTTTCGACAAGAAGAGTTACGCCGATAGCAAAGATGAAGAGTCGAACGCTGCTCTCTCAGACTTCGCCGAAGACAAAAGGGGTTTCTTCGGCAAGCGATACTACGGCAGTAAGCGGGAAGAAATCAGTCCCAGGGTGTTGAATCTTCTTCTGAGAAATTATCAGATCCACCCTAGTCCTCTCAATGGACACAACGCTTTCAGCAGGCTTCTCGCGAAGCAGGGATTTTGGGAGAACTGA Protein: MTSSAQCLLLTMLLVATLDFLYAEDIGFAETDSELTPFAGAVADHVLKEGFGKRGYFDKRGFFDKRGDAS KRGFFDKRGFFDKKSYADSKDEESNAALSDFAEDKRGFFGKRYYGSKREEISPRVLNLLLRNYQIHPSPL NGHNAFSRLLAKQGFW EN Adipokinetic prohormone type 1 precursor Gene Bank Accession Number: n/a Reference: n/a Partial clone: CGCAGTCGGTACTTTTTTTTTTTTTGTCTGGCGTTCTTTGGCGAAAGTGTAGAGCTCTCAGGCGTATAAAGACCAAGCGATCAGCATCATCAAATCAACTCTGCTGCAGCTGAGCCAATGCTTTCACACATGTCGTCACAGTTCTGGGCAGGTATTCAGGCAGGAAGAGAGTTTCTTTGCGGTTCGCTGTATTAAAAGCATGATAT

88

CGATGATTTCTTTGTCCGCGATCTGCCAGCAGTGTGGGATTTCCTTCTCGGTGACAGTGGAGACCGCCCTCTTCCCTGTACCCCAGTCGGGCGAGAAGTGAATCTGTGCAAGGCAAGTGGACGTGCCTATCACAAGGACGACAAGGATCAAAAGTATGCTAGAAGATTCCATTGTGCTTGCTCTTGTTTTCGTGTGACACCAGGAGAGCTCCTTGAGCTAGCAGAGACCAGACTGTCTTGGAAACTCAGTTAGGTCTGCGGCTCCTAAGACCCCC Allatotropin-0F precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Prediction: GGCTCGAGGCAGAGCGAAGCGAGGTTTCCGCCTCAACTCTGCGTCACGGGTTGCCCATGGCTACGGCAAGCGTGGGTACGCTTCCTCTTCCGGTGCCGTCCCTTACCCAGAGTTGGCGAGAGATGTCCTAGACAACTTACGAGCAGAAGAGGAGGAAAAAGAATTGGAGTGGTCAATCATGAGCGTGGATGAGCTGGCGTCTCTACTGCAGTCACACCCAAAGCTTGCCCGCGCCTTAGTGAAAAAGTTCGTGGATATCAACGGTGACAACTTAGTTACGGCAGAGGAACTTTTCCGGCCCCCCACGAGAGGGAACTATATTGACATCGCTCTATCTTTTATTTTTTTATTTTTGTTTTTGTTTCTCTGTCCAAAGAACAGCTGATGGGACCTCTGGAATTTTTTCTGAGTTGATGATACTTTCGAGGTATGGATCTTTTTTCGTCTATGAATGTCTGAAAATAACATTGTCCATATTACTGTTTGGCCCCTTTGTTCTCACGACCAAGGCTTGAGATTGTTCCTCCCTGGAAAAGAAGAAATTGGACATTCTGACTCTTCTGGAAATACGTTTTGATTACACTTTCAAATTAACAATGTGCTAATAAACTAGAACATGTCTGAGGTTCCGTTTGCAAAAGAA Allatotropin-0R precursor* Gene Bank Accession Number: n/a Reference: n/a Partial clone: GAATTCGCCCTTCAAGTGGTGATTCGGCCGCTTTTCTTTTGTACTCGCTTCTACAACATCGGGGTGCAGTTCCCAGGACAAATTCGAGCACCGAGGTCACCGACAAGACTAAAACTCGAGAACACCAGATCTACGCCTGTGACAACCCGATCGACATACACTCCAAGACAAACCAGCACACGCCCAAGACCCGCCAAAAGCCTAGTCCTCACAATGCTCAGCGCACCCTCCATAGCCCACACAGGGGTCGCCCTCCTTGTCCTGATGTGTCTTTGTCCCTTCTCACAGTCTACAGAGGCGTCATTAAGCAGAGCGAAGCGAGGTTTCCGCCTCAACTCTGCGTCACGGGTTGCCCATGGCTACGGCAAGCGTGGGTACGCTTCCTCTTCCGGTGCCGTCCCTTACCCAGAGTTGGCGAGAGATGTCCTAGACAACTTACGAGCAGAAGAGGAGGAAAAAGAATTGGAGTGGTCAATCATGAGCGTGGATGAGCTGGCGTCTCTACTGCAGTCACACCCAAAGCTTGCCCGCGCCTTAGTGAAAAAGTTCGTGGATATCAACGGTGACAACTTAGTTACGGCAGAGGAACTTTTCCGGCCCCCCACGAGGAAGTAGCTCTTGCCGCCTCATGGAAGGGGGAGTTGCTACGCCTGTCCCACATGAGTTGGCTCAATTATCTGCCCTTTGCAGGTCTGGGGTCCACGAGAGGGAACTAAGAGGGAACTATATTGACAAGGGCGAATTC Antigen-5-like protein precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Prediction: TTTTTTTAAGGGCTACGGTAGATGTTTATTTTCCTCAAAACTGAAACACCACCAGATAGCAGCCCGTTACATAACCGACTTGCATCTAGGGCTAGTTTATCTTCCATTGCGCTGGGTGTCCAATCTGCCAACAGTCACCAGTTGTCGGCCGGAACACCCGCGGAACTGTAAAAAGTTCAGTCGTCGCCGATCCACACAACCACTTTCCAGCTGATTAGCTGAAGTTTGAATAACAAGAAACCGAAGCTTCTAGAATGGCTTCTCACCCATCCAATTGCCAGGTGGGTAGTAAGCACAGACAACCAGCGTGCCCAGGTCCCAGGTGTGGTCACAGGTAGACACGGCACAGCCCACCTGTCTGGTGTCTCGCCACACTACCTGAGTGTAGTGACCACAGCTTCCATCAGTCATACACCTGTAGGTCCCGTCGTTGTAAGCTCTCTCACTGACCCACATCTCTGTGGCCTGTGCGGCGACTAACCGGGCGCTGATGTGGCGATTTGACGTAGCCATGATGTTCTCTCCCCAATACCTCCGGCTGGCCGGTGTGTGGCTCATGCGACATGTGGAGGCCAGGACGGACACTTTGTTAGCCAGGTCATCACTCCACGTCAGA

89

TCCCTCAGCCCCGCGCCTTCCTGTCTCCTGGCCTGGTTGTGGGCGTCAAGAAAAGCCTGCTTGTCTCCCCAGGTCAGGGACTTGAGATGACCGCCCCAGCAACACGTGACCAGACACAGGACACTGACCAACACACTGACCGCCGCTCTCCTCATCCCGACAGACGTGGACACAACTTTTCGTGGAGGAGGTCAGCTGATGTTTCGGCCAAGCCAGACTCGAGCCGCCTCGAGCC Appetite regulating hormone precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Prediction: CTCTGTTTTGCTTTTTAAAATGATCCTTGCAAAACTAGCAATGTTACAGGGAAATGGTTTTCTATCTTTAAATATGACATTAAATGAGATGATGACTCCAGGAAAA Astacin-like protein Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: CTTGAGATCAAAGAAGGACATTGCCTGCCTCTGGCCCAGGTCACGTTGTAACAGAGGGTCACGTGTCTCCAGGGTGATGCGGCCGTTCTTGGAGAAAAACGAGGAGCCGTAGTGCATGATAGATCCTACGTCATAGGGCACTCCCATGTTGTCTATGTCGCCCCATGATTCCCGTTTAAAGTTGAACTCCTCCCCTCTCTTCACGTTCTCCTCCAGGTATGTGACGTAATCATCTCGGTCCGGTCTGGACTGCTCGTGCCAGAAGCCCAGGGCGTGGCCTAGTTCATGGAGGAGGACTCCTTTCTCAAGGCAGCCTTTGGCCGTGGAGACTTCCTGGTACTTGAAGGCTTTCTCTCGACCTACATATGACCAACACCCCACGTCTTTACGGAAGTCCAG Atrial gland-specific antigen precursor 2 (AGSA) (Mollusk-derived growth factor) (MDGF) Gene Bank Accession Number: 155709 Reference: Sossin, W. S., Kreiner, T. et al. (1989). A dense core vesicle protein is restricted to the cortex of granules in the exocrine atrial gland of Aplysia california. J Biol Chem. 264(28), 16933-

40.(Sossin et al., 1989) Bradykinin-like neuropeptide (LUQ-1) Gene Bank Accession Number: 155765 Reference: Wickham, L. and Desgroseillers, L. (1991). A bradykinin- like neuropeptide precursor gene is expressed in neuron L5 of Aplysia californica. DNA Cell Biol. 10(4), 249-58. Buccal in precursor Gene Bank Accession Number: 404497 Reference: Miller, M. W., Beushausen, S. et al. (1993). The buccalin-related neuropeptides: isolation and characterization of an Aplysia cDNA clone encoding a family of peptide cotransmitters. J Neurosci. 13(8), 3346-57. Central nervous system APGWamide Gene Bank Accession Number: 4099286 Reference: Fan, X., Croll, R.P. et al. (1997). Unpublished.

90

ChordinGene Bank Accession Number: n/a

+

Reference: n/a Partial Clone: AGACGGGAGAAACAGATTTTGTTGTGTGATAACGTCAGATTATCTCCTCGAGGGAAACGCGCGCTGAGAAATAGTAAATAGTGAGCCGAGGAATTTATCTATCGTTTTGTGCTTCTTCGCGCAGTCTTGTAATTGAAGAGTGGGCCTCCTTCCAACTTTTCTTCCCTTCTTCTGTTTGACATCACTGACCGCAAGTCGAGTGTTCTCTGGTGTTGTGTGTTTCTTATAATTGTGGAACTACTGCAGAGGATTTGTTGTATGGACACTTCGGATTTTTCAGACGTTTGGAAATAATGCGCGAGTGTTTTGACTGATTTCTGTACAGTACCTGGAAAATAAACTACGCACGTTCTGTGATTCTTACGGTACACTTGAAATTACTGACAGACAGTTTTCCATTTCCTGACGCGACCCCGTTATAGGATGGGGCAACCGCCTACTCAAGCGGGCTGCTCTAGCAACTCGTGTGTCTATGAATGCGTCCGTGAGTCTGTTCGTGAGTCTGTACATCGTGGGTCAAGTCACATAAAGGTGGTCAACAGCTGAGGAGGTCTAGAGAGGACGAAACCAGAGACAGCTGCCAGCCGCCCATAACATGCAGAAGATGACTCAGATGCTGGTGTATAAAGTGCTCATCTTCGTCATGGTATATCGCTGCAGTGGAAAGCCTTCGGGAGGCCCGTGTGTTTTAGGCGGAGAGCAGTACAACGTTGGAGAGACGTGGCATCCTAAAGAGTTTTCTTCCACCGCCAACTCCTGTGTCCACTGCACGTGCCTAGAGGGCGGTCAGATAAATTGTACCGGCGTGGACTGTCCTTCTCCAGAGTGCGAGGTGCCCAGATTTATCCACGGACAGTGTTGTCCTACATGTGATGTCCGGGGGGACGAGGCCGGAAGTCCCGAGACAGACATGCCCGGATGTGATTTCCATGGCGACCACTACGAGGACGGTGACATATTTCCGTCCAACAAGACGGCTTACAAACCCAAGCATGACAACCAGTGTGTGTTGTGCGGGTGTTATAGAGGTGAGGTCATCTGTCACCTGAAGACTTGTCTGCCCAGCCCCAGGTGTCGGCGGGTAGTCCGGGTGGATGACGACTGCTGCCTACAGTGTGAAGAGGAGTCCAGCATAGACGAATATTTCATGTCTATCGGGTCAGACAACATAAACCATACACTGGACGACGAAGACTGTCTGTCAGCGACAGGACGCCGTAAGAACGGCAGTACGTGGAAGCCAGTGGTGGGCGAGTACGGAGAGATGCATTGTATCGTCTGCTCCTGTTTAGAAGGTCAGGTCGACTGCAAGCGTCTGACCTGCCCTGACGTCACAACCTTGACCTGCAGACACCCAAGGCCACGACAGGACGGGTGCTGTAAGGAATGCCCTGAGAGCGACAGACAGAGGGACAACAGGAAGAAGAAGAGAAAGAAGAGTAAAAAAGG ConopressinGene Bank Accession Number: FJ172359

*

Reference: n/a CDS: GGCTCGAGGTTTTTAAAGTTTGCATAAGTTCTCCACTGGACTCAAAACAAAGAACTAAACGAGAGATTAGAGAACGAAATCAAAACGCTATTTACCAACTACAGGATGTCTCACTCAAGCATGTCACCACTTTCTGTTCGGACTTTCGTCTTGGTAGCAGGACTTGCCGTGATTTCGTTCTCTGTCGTTGCTGATGCCTGCTTTATTCGGAACTGTCCTAAAGGAGGCAAGAGATCAATGGACATGCAGCTTCTTGGACAGCGACAGTGCATGGCGTGTGGTCCTGAAGGGATCGGCCAGTGTGTAGGCCCCAACATCTGCTGCAGTCCACATTTTGGTTGCCATATTGGCACACCAGAAACGGAAATATGCCAGAAAGAAAATCAGAGTACTTCCCCTTGCAGTGTCCGAGGTGAGACGTGCGGATACCGAGACTCAGGAAACTGCGTGGCTAACGGAATTTGCTGTGATTCAGAATCATGTGCCGCAAATGACAGATGCCGACTTCGAAAGGAAACGTCTAGAATCGGCTTTGATGACACACAGAGCTCACGCGCCGAAGTGCTCAAGTTGATACAAAAATTACTCCGCGCTAAGGAGGAAGACTGACACTGTATCCACAAAACACGTCACAGTGTCCACTCAACGTCATTTCCGGCAAAGACAACTCTAAGTGCCCACTGACGTGAGGAGCCAAAACGGGATTACTTTTTCTTGTGATTGTTTTCGCTTTTTATTTGTTTTTTCTGGAGTTCACTGTCAACTTGCTTTGGGACAACTACCTTTCTGTGAATAATTCGGAGAAAGACGGGAGTATCTATTAGGACTAGGAGACAAAGAAGTACTGTATGAGTCATGTGTGCAAGGGTATTAAAATTTAAAAAAGATAAAACGTTTTTGTGGCTTGCTGCTACTCGCAATGTCAATTATTATCTTTCATCGCTCGGAGTTAACAAAGGCTGTTGAGGGCCACCCTGTTTCTGGATGTTCTGATAAAGAAATACTCTTGACTTCAAAGACCCAAAGCAGTGGAAGCAGAATAAACAACCTTAGTTGCGAGTCTCTCGTCTAACCAAATTACGTAGGATGCCTCGGGAAAACAAGGTGTGATGAATGAGAATGGAATCCTGAGCAAAATGGTTAGAACGGTTATTGACAGCCACAATGGCACATTGGACATAGAAGAAAGATTTATAGACAGTACTCATGACTCAGTACGCAATATCACTACTTTTTAACTTAGCTCATTTAACATGAAAGAAAGAGTAGAAGTGAGTTGATATAGGTGGAGGGGAGGTACACTCTTTTGAGAAAATAGGAGGCGTTAGTCACAACACGTACTTATTTTTTTAAAAGAAATAGGCACTCCTTCTTTTGTTCAGTGGGAGGGGCTAAATTGTGTTTGAGGCCCGTATGTCATCTTCTGTGGCGAGCAGTTTTTGTCTTATGATGAAAACTGTCAGGCGATAAATACTACTTGAATTTGTATCCGATGATCC

91

ATGATTGTATAAAAAAACAAACAAACGAAGGGCATCTCCAAGAGTTCAGCTGTCTCATATTATGTCCTGTTGTGTTCAATACTGCCACAGATCACGAATAATGTATTTCATGTGCACTTCAGTATCCATCTTTAATTAGAAAGAAAAACTAAAACAAACAAATGGAAAGAAGATTGAGCGGTTCAGGTGTACCATATCGGCAATGAAGTCGCTAAGAAATGTTTATGAAAGGGAGTTTAAAAACACAGAAGGATTAATCTAGAGGGTATAAAGGGGTGGGGATGGGGGTTGATATGGAAATAAAAACAAGAAAAGAAGACCAGCAAAGGATATTATGAAAAAAATGGTTAATTAACGATGTTTCGGATATCCATGTCTGAACAAGGGAAGTAAGTCTGGTAAATTGTGGCATAATCTAAAGCTTGCTTTAAAATTGAATTGCTTCCCTTTAATTAAAAATGTGTTGGTTTTTTTCCCAAAAAAA Protein: MSHSSMSPLSVRTFVLVAGLAVISFSVVADACFIRNCPKGGKRSMDMQLLGQRQCMACGPEGIGQCVGPNICCSPHFGCHIGTPETEICQKENQSTSPCSVRGETCGYRDSGNCVANGICCDSESCAANDRCRLRKETSRIGFDDTQSSRAEVLKLIQKLLRAKEED Corticotropin-lipotropin precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: CTAAAAAGCAGATGTACCCTTTGAAAGCATTTTTTAAAAGCATTTTTGAACAGTGTAAGCAGTATTCATTTAGG Delta-like precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: CTTGTGCTGGCTTCGAGTGTTTGAACGGAGGTTCCTGCACTGCACCTGCCGACTTCCCATCCTGCCTATGTCCACAAGGGTATTACGGAGACAAATGTCAATTCAGACAAGTAACCAGGGACCCTTGTGCTAACTTTGAGTGTTTGAACGGAGGTTCCTGCACTGCACCTGCCGACCTCCCATCCTGCAGTTGTCCAGGAGGATATTTCGGGGACAAATGTCAATTCGGACCAGTAATCAGGGACCCTTGTACTGGCTTCGAGTGTTTGAACGGAGGTTCCTGCACTGCACCTGCCGACTTCCCATACTGTATGTGTCCAGAAGGATATTCCGGAGACAAATGTCAATTCGGACCAGTAATCAGGGACCCTTGTGCTAACTTCGAGTGTTTGAACGGAGGTTCCTGCACTGCACCTGCCGATCTCCCATTCTGCAGTTGTCCAAAAGGGTATTTCGGAGCCAAATGTCAATTCTCATCAAATCCCTGCGCTAACTTCAACTGTTTGAATGGAGGTTACTGCTTTTCATTTTTCAACCGCCCATTCTGTTTGTGTCGCTTTGGATATGTGGGGAAAAAATGTCAAACAAGAATCTATTAACGATTCCTAAGACAGTGTGCTGTGCCTTTTGCGAGTGATATCAATGTGGTTGATCCCAACAAACAGAATATATGTAATATCAATAAGTGAATTCCCACTCCGCCTTTTCCAGATTTCTTATTATGTATTTCAAAAATCCTCAGCTTCCTTCTATTATCCGTAAAGAGAAACCTATACATGTACTGATTTCGTCAAGAAGAATGGGCTGAAAATAGCACGATGTCAAGTTGGGGTTTTTCCAAACCCATACGTGTTTAACGATAACATGATGCAAATCCAGTTGACAGGAAACGTATTTCTATTACAATATTGTGATTTTAGAGACAATAAACTTTATTAGTGCCATCAACAAAAAAAAAGTAGGGACTGCGCTGCGTGGATACACTGCTTAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATATGATCGTCATCGGCCCCCCCCCCTTAAAAAAAAAAAAAAAAAAAAAAGNNGGGGGGGCCCCGGCCGCGCGCGGACAAAAAAAAAATAAAACCCCCCCCCGGGGGGGGGG Dorsal-ventral patterning tolloid protein precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: GGCTCGAGGCGGCTCGAGGCGGCAAAGGTTTCAACGCAGCCTACGAAGCTATCTGCGGCGGAGAGATCACCAGAGAGGAAGGGTTCCTCACCTCCCCCAACTACCCGGATGACTACCGGCCCGACAAGGTCTGTGTGTGGAAGATCACCGTGGGTCCTGACTTCACTGTGGCCCTCAAGTTCCAGTCTTTCGAGATTGAGAACC

92

ACGACGACTGTGTCTACGACTATCTTGAAGTCCGTGATGGCCCCTCTGAAATGTCTCCCCTCATCGGCAACTACTGCGGCTACAAGATCCCAGAGGACATCAAGTCCACCGGAAGGCACCTCTACGTCAAGTTTGTCAGTGACGGCTCCGTGCAGAAGGCGGGATTTTCTGCCACTTTTGTCAAAGAGTACAACGAGTGTGAGAAGGAGGAGCATGGCTGTGATCACGTGTGTGTCAACACCCTGGGCAGTTACCGCTGCTCCTGTAGGATCGGCTACGAGCTGCACTCTGACGGCAAGAGGTGTGAAGATGCATGCGGCGGTTACATTGACCAGGAGAATGGCACAATTACCTCCCCTTCCTACCCGGACCTGTACCCGCCCAACAAGAACTGCGTGTGGCAGATTGTTGCCCCTGACGACCACAAGATCAACATCAACTTCACTCACTTCGACCTGGAGGGCCACAATCAAGACTGCGAGTATGATTCAGTGCGTGTGAGCAGTGGAAAGGGGAAGGAACTCAAACTACACGGCGTGTTCTGTGGCTA CACGTTTCCAGCTCCAGTGACGTCAGA ELH Atrial gland peptide A precursor (ELH-18) Gene Bank Accession Number: 119272 Reference: Mahon, A. C., Nambu, J. R. et al. (1985). Structure and expression of the egg-laying hormone gene family in Aplysia. J Neurosci. 5(7), 1872-80. ELH egg-layi ng hormone-related precursor [Pro 25] B Gene Bank Accession Number: 2599363 Reference: Kurosky, A., Gorham, E. L. et al. (1997). Expression and genetic variation of the Aplysia egg laying hormone gene family in the atrial gland. Invert Neurosci. 2(4), 261-71. Endothelin-like precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: GACAATGTGTGCGATTGTGTGTCTCCTTGTGTTTTAATGCTTGTGCTCTGTGTATGTGTAA Endozepine-related-1F protein precursor (binds GABAA receptors in the central nervous system, and it increases the mitochondrial synthesis of pregnenolone.) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: GCCGCATGGGGTGAATATCGTACAGGCCGGATAAGTTTGAGGCAGCGGTGAAAGTAATTCGTGGTTTGCCCAAGAATGGTTCTTTCCAGCCTTCCCATGAGCTTATGCTCAAATTTTACAGCTATTTCAAGCAAGCCACAGAAGGACTCTGCACATCTCCAAAGCCAGGTTTCTGGGATTTGGTCAATCGCAAGAAATGGGAGGCGTGGACCGATTTGGGTAAAATGGAAAGCGAGGAGGCCATGCTGCTGTATGTGGATGAGTTGAAAAAAAAAAAAAGTACCGACTGCG Enterin Gene Bank Accession Number: 74844518 Reference: Furukawa, Y., Nakamaru, K. et al. (2001). The enterins : a novel family of neuropeptides isolated from the enteric nervous system and CNS of Aplysia. J Neurosci. 21(20), 8247-61. Enterin neuropeptides precursor 2 Gene Bank Accession Number: n/a

93

Reference: n/a Comment: Predicted only, not cloned Partial: GGCTCGAGGGTTACTCTCATAGTTTTGTAGGTAAACGAGTACCCGGCTATTCGCACAGCTTTGTTGGTAAACGAGTTCCTGGTTACTCTCATAGTTTTGTAGGTAAACGGGTACCCGGCTATTCGCACAGCTTTGTTGGTAAACGAGTTCCTGGTTACTCTCATAGTTTTGTAGGTAAACGGACTCCTGGCTATTCGCACAGCTTTGTAGGTAAACGGGCACCTGGCTATTCACACAGCTTTGTTGGTAAGCGGACACCGGGTTATTCCCACAGTTTTGTTGGTAAACGGGCACCTGGCTATTCACACAGCTTTGTAGGAAAACGAGCACCAGGTTATTCCCACAGTTTTGTAGGTAAACGGGCACCAGGCTATTCACACAGCTTTGTTGGTAAGCGGGTACCGGGTTATTCCCACA GTTTTGTAGGCAAACGGGCACCAGGCTATTCGCACAGCTTTGTTGTTAAGCGGGTACCGGGTTATTCCCACAGTTTTGTCGGTAAACGGGCACCAGGCTATTCCCACAGCTTTGTAGGTAAACGAGAACTAGGTGAGGATGAGATAAACTTTCTAAAAGAGGTAGACGCAGCAGACATTTCTAGACAACTTGCAGAAGAAGACGAAAAGGAAACAATGGTATCAGTTGATGATAAAGAGACACTCAGCAACGAAGAAGACGCATCTGAAGACGATTTTGAGAAGCGGGAAAAAAA Ependymin-related proteinGene Bank Accession Number: 94434843

+

Reference: Moroz, L. L., Edwards, J. R. et al. (2006). Neuronal transcriptome of Aplysia: neuronal compartments and circuitry. Cell 127(7), 1453-67. CDS: ATGAAGTTTCTGGTAGCCTTTTTCGTCTTGTTCGTGGCATGTGTCTACGCCAATAGGCCATGCTCCGCTC CCCATGAGCTCACTTTCCGCGCCTCCAGATGGAACTATGGCGAAACTTACTACGAGCGGTTCTTCGGAGAGTACGACAGATTCAACAAGAGGGTAGTACTCTTTGAGGAGGACTTCGAAAGCGGCACCAAAAAACAACGGGAGCTCCTGTTCCTTCACAAAGAAGGTGTAGGCTACGACTTCAACCTGCAGAGCAAACATTGCAAGAAGTTCAAGGTCGGAGCATTCAAGCCCTTCGAGGTGCCTTCTGGCGCCACTTACGAGGGCGAATTCTCCATTGGAGGACCCACCGAGGAAGTCACAATCGACAGATGGTCGGACAAAATCAGTTCTCGTCGTGAACACTGGATTGGGGAGTTCTCACTAAAGACCTGCTACCCTATCAGCCAGTTTATGATCGAAGAGGGTAACTTCAACAAGACATCCTTCACCTTCTTCTACGACACCGTCAAGGGAATCGTCAACCCCAACGACTTCAATGTGCCCAAAGAATGCGAGAAGTCGGCGATTGAAGTCGATATGCCAATCAACGCTCGCCTTGCCAAGGCTATGTACTCTGGAACCCTGCATGACTAA Protein: MKFLVAFFVLFVACVYANRPCSAPHELTFRASRWNYGETYYERFFGEYDRFNKRVVLFEEDFESGTKKQR ELLFLHKEGVGYDFNLQSKHCKKFKVGAFKPFEVPSGATYEGEFSIGGPTEEVTIDRWSDKISSRREHWI GEFSLKTCYPISQFMIEEGNFNKTSFTFFYDTVKGIVNPNDFNVPKECEKSAIEVDMPINARLAKAMYSG TLHD Ependymin-related protein-2* Gene Bank Accession Number: n/a Reference: n/a Full Length Clone: CDS: CCTTAATACTTCTGTCAGCCCTGGTATCAGAGCCACTCACCTCTA GTCCAACGTAGCTGGTAGACGTGAGAAGATACACCATGTTGAAGCTTCTGTTTTTCGCCCTTCTGGGATTGACACTGTCTGTCGCGGACATCCCTCGTCACTGCTTCTCTCCCCCTGAACTCACGTTCAGAGCCTTCCAGTTCGACCACGAGTATACCACCTTCAACCGTTTCAAGGCCGAGTATGACGTCAGAGACCGCAGGGTGGCTTTCCTGGAGGAGGAAGTCAAAGGACCGGCACCCGGAAAACAATACCTGTGGCTCATGTTCCTGCACGAAGAGAAAGCAGGTTTTGAATTCAACCTGAAGACCAAAAAGTGCAAGAAGTTCAATCCCGGCAAATTTCATCATTTCGGCACCCCAGAAGGTTCCAAGTTCGAGGCTGACTTCTACGTTGGCGGACCCGGGGAGTCCATAGACGCCGAGATGTGGTCTGACAGAACCGACTTCAAAAGGGAAGACTGGCTAGGCGTGTTCACCAAACGTAATTGCTACCCTATCCGCACATTTACGAGGAACACACACAACAACCACACCCTCACCACCAACATCAACGACCTCGTCGAGGGCATTGAGGACCCAGGTCTGTTTGACCCTCCTAAGGAGTGCTTACAGCAGGGTGTGCTAGAAGAAGAGATGAGCGAGACAGCGCGCAGAATGTACAGCATCTACTCACGTACTTTGTTGTAGATCACAAAACAGGCA

94

TGCACAATTATTTTGTTTTAAGTTGTTAAAATGCTTATGTCCTTACGTCGTTCTAGTATGTCTTCTTCTCGAAAATGTACATGCACGTATACATTCAACCATTTCTAATTAAAGCATGCATTCACATCTTGTAACACGTATATGTGTAATTCTAAGTCGATG Protein: MLKLLFFALLGLTLSVADIPRHCFSPPELTFRAFQFDHEYTTFNRFKAEYDVRDRRVAFLEEEVKGPAPGKQYLW LMFLHEEKAGFEFNLKTKKCKKFNPGKFHHFGTPEGSKFEADFYVGGPGESIDAEMWSDRTDFKREDWLGVFTKRNCYPIRTFTRNTHNNHTLTTNINDLVEGIEDPGLFDPPKECLQQGVLEEEMSETARRMYSIYSRTLL Feeding circuit activating peptide precursor 3* Gene Bank Accession Number: n/a Reference: n/a Partial Clone: TCTTTGGGGAAAAAATCTGATACTGAGGAGAAAAGATATTTAGACCATTTGGGAAGTTCTTTGGTCAAAAAGTCTGATACTGAAGAGAAAAGATATTTAGACCATTTGGGAAGTTCGTTGGTGAAAAAATCTGATACTGAGGAGAAAAGATATTTAGATCATTTGGGAAGTTCGTTGGTCAAAAAGTCTGACAGTCAAGAAAAAAGATATTTAGACCATTTGGGTAGTTCACTGGTTAAAAAATCTGACAGAGAAGAAAAAAGATATTTAGACCATCTGGGAAGTTCGTTGGTGAAAAAATCGGAGAGCCCGGAGTTGGCAAGATTTGAGCAGCGGATGTTTACAGACTACAACAACGACCTTGTTGCTATGGAGAAAGACTTCCTGGAGAAGAACAGAGAACTCCAGCAGTCGAGCCCGGATGTGACGTCACAGGGGAACAAACGTTATCTGGACAGGATCGGCAGCTCGCTGGTGAAGAAGGAAGACGACGAGGATACAGAGGTGGACGAAGACGGAGTGGGGCTATTTATAGATTTGGAAAAAGATGAAGATGAGAACGACTTGAGGCAAATGGCGGGCACGA GAGCAGGTAACCACAAGAGATATCTGGACCGTGTGGGCAGCTCGCTCATCAAGAAACAGGCGCAGGCTCAGAAACGATACCTAGACGGGATCGCAAGTTCGCTCATTAAAAAGGAAGACAGAGGCCAGAAACGATATTTAGATGGAATCGCAAGTTCACTCATAAAAAAAGAAGACAGAGGCCAAAAACGATACTTGGACGGGATCGCAAGTTCGCTCATTAAAAAGGAAGACAGGGGTCAGAAACGATACTTAGATGGCATCGCAAGTTCACTCATCAAGAAAGAGGATGGTTTCGTTGGCGACATCCCAAACGGACAAGAAAAACGATATTTGGACGGTATCGCCTCTTCCCTGATAAAGAAAAATGAACTAAAAAGCGAAAAGCGTTATCTGGATGGTATCGCAAGTTCCTTAATAAAGAAAGACAACGTACAAGAAGAAAAACGATACCTCGATGGCATTGCCAGCTCTTTGATTAAAAAAGAAGTCAGACCCGGACAGAAACGATATCTAGATGGTATTGCAAGCTCTCTCATAAAAAAGGACTCGACAAACGAGAACAAGCGATATCTGGACGGTATCGCCAGCAGCTTGATCAAGAGACAGGACTCGGTGAGCGATGAAGAAAAGCGATACTTGGACAGTATCGGCAGCTCTCTCGTGAGGAAATCGTCCTCTACGACTCCGTACAAACGATACCTGGACCAGGTTGGGAGCTCCCTGGTGAAGAAAGGGGCATCAGAGATGGGACCATACGAGGTGATTCCCGAGGCTGTCTACGTGGGGGAGGGGATCTCTGCGGGCGGGGAGGGGCCGGTGTACGTCACCATTGACGGTCAGCTCCCCATGTTGGCCATGTCCGAGACTGACCTGCCAATGGCCAGCCCCAGGAAGCGGATGTACGAGGTGACAGATGACGTCATGACCGGAAGTGGTCACCAACAGCTGGTGGACGATATGGCGGCGGCGATCCACGTGTCGGCCAAACGGGCAGGACTGACAGGTAGGGGCAGCGTGGTAGGGCGATCTTGTCTTATCTTGAATGTCGTTGTTGTCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATTATTTAGAACCTTCTGCCTCACCACTAGCTCACATGTTGACCAGGTCGAGTGCCGATTGGGTAAACGTTTTGTAACGTTTTATTTACGTGGCGTTAGTGGCCTAGTGGTTAGGCTCATCATCATCATCCAGTGCCAGATCTGCGA CAGAGATCGGCGATTATGGTTTTCCATTCATTCATTTGCTCGGATGCTCTCAAAAAAGCTACGTTTCCCTGATCTTTCCCAGGTTAGGCTACCGGACTCGTAATCGTGAGGTTGTCGGTGGCCTAGAGGTTAGGCTACCGGACTCGTAATCGTGAGTTTGTCGGTGGTCTAGTGGTTAGGCTACCGGACTCGTAATCGGGAGTTTGTCGGTGGTCTAGTGGCTACCGGACTCGTACTCATGAGGTTGCGGATTCGGGGGTTCGAGCCGTGGGCCCGACGTTGTGTCCTTGGAAAAGGCACTTTACACGAATTTTCCTCACTTCACCTAATTGGGAATGGGTACCCAGCTATAGACATCGAAAGGTCTTCTCAGTATGTTAATGTTTTAGCTCCTATAAATGGCTGCATGCACTGTATGCATCCCCGAAAGTTGAGGTTGTTTTAGAATGCTATCGGGTCTGCTGGGGTAATAGTATAAGTTGTGAAACGCAAAGAGATTGCTGTTGAGCGGGAATTTGCGCTATACAAATGCTATCTATTATTATTTATGATAATGGTTACGAGTTTCGATCCTACCTGTCAGGGCCTACCCGGGCTAAAAGGGATATAAAATCCCGCTAGAAAAACAGAGATACTCGATAAACCTTCTCCCCACTCGGGAATAGAACTCCTTTCTCCTGTGTTCGACAGCGTAACGCCTAACCTCTACACCAAAGAAGCCAGACAAAATGGTTGTATCACAGACTCTAATGTGTCTCACGTCCATTCATTTCAGTA

95

Feeding c ircuit peptide 2Gene Bank Accession Number: n/a

+

Reference: n/a Partial Clone: CTGGACGGTATCGCAAGCTCGTTGATCAAACGGCAAGACGTGCTGGGTTCTGACGTGGCCATTGGGAGCACGGGGAACAGCGGGCCGGACAAACGATACCTTGACCACTTGGGTAGTTCACTGGTCAAGAAATCTGATGGACAAGACAAACGATACCTTGACCACTTGGGTAGTTCGCTGGTCAAGAAATCTGATGGACAAGACAAACGATACCTTGACCACTTGGGCAGTTCTCTGGTTAAGAAATGGGACGAGGGTGACCAGGAAAAGAGATATTTGGACCACTTAGGCAGCTCACTAGTGAGAAAATCAGATAGTCAGGACAAGAGGTATTTGGATCATCTGGGGAGTTCTTTGGTCAAAAAGTCAGATGGTCAAGAAAAAAGATATTTAGACCATTTGGGTAGTTCA CTGGTAAAAAAGTCTGACGGTATAGACCAGGAGAAGAGATATTTAGACCATTTGGGAAGTTCTTTGGTGAAAAAATCTGATACTGAGGAGAAAAGATATTTAGACCATTTGGGAAGTTCATTGGTGAAAAAGTCAGATGGTCAAGAAAAAAGATATTTAGACCATTTGGGAAGTTCTTTGGTCAAAAAGTCTGACGGTATAGACCAAGAGAAGAGATATTTAGACCATTTGGGAAGTTCCTTGGTGAAAAAATCTGATACTGAGGAGAAAAGATATTTAGACCATTTGGGAAGTTCTTTGGTAAAGAAATCTGATACTGAGGAGAAACGATATTTAGACCATTTGGGAAGTTCTTTGGTGAAAAAATCTGATACTGAGGAGAAAAGATATTTAGACCATTTGGGAAGTTCTTTGGTCAAAAAGTCTGATACTGAAGAGAAAAGATATTTAGACCATTTGGGAAGTTCGTTGGTGAAAAAATCTGATACTGAGGAGAAAAGATATTTAGATCATTTGGGAAGTTCGTTGGTCAAAAAGTCTGACAGTCAAGAAAAAAGATATTTAGACCATTTGGGTAGTTCACTGGTTAAAAAATCTAACGGCATAGACCAAGAAAAAAGATATTTAGATCATCTAGGCAGTTCTTTGGTCAAAAAGTCAGACAGTGAAGAAAAAAGATATTTAGACCATTTGGGCAGTTCGCTGGTTAAAAAATCTGACAGAGAAGAAAAAAGATATTTAGACCATCTGGGAAGTTCGTTGGTGAAAAAATCGGAGAGCCCGGAGTTGGCAAGATTTGAGCAGCGGATGTTTACAGACTACAACAACGACCTTGTTGCTATGGAGAAAGACTTCCTGGAGAAGAACAGAGAACTCCAGCAGTCGAGCCCGGATGTGACGTCACAGGGGAACAAACGTTATCTGGACAGGATCGGCAGCTCGCTGGTGAAGAAGGAAG FFELamide/FMRFamide -3 Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: GGCTCGAGAGGACCCTTTTACCCTTTCGGTAGAGGCGACCCATTTATCAGATTTGGTAGAGGCGTCCCCTTCATTCGATTTGGTCGAGGCGATCCATTCATCCGATTTGGCAAAAGAGGAGCAGACAGCGAAGAAGGTGTCGGTGAAGGAGAAGCCTTGTCGAGGACAGCCAGATCGACAGAAACGACACAGGAACGAGCCAACTCTCGTCAAATACGCAGTGCAGACGCCAACAAACGATTCACGAGGTTCGGCCGCTCCGCCACTTCCGCTTTTGTGACCCAGCAGGAGGCTGAGGTCAAGGACGCAGTGAGGAAAAGGTCAGTCGAGAACTTGGACAACGAGGTCAAACGAGCGATGTGGAGCAACAACCAACAGATGACGCGATTGTCCAAAAGAGGTCACCCAGAGTTCATGCGATTTGGGCGGAGTCAAGGAGACGAGACAGAGGACGACGAAATTGGCGGAGATGACGTCATCAGTTTGGGGGAAGGTCTAGCACAGAGGGTGCGCCGGGTGTACGAAGACAGGAGCTCACTGCCACGATTTGGGAAGAGGCAGGAGGTGGAGGAGGAGGAGGAGGAGGACTTTGGGAAGGAAAAGAGACAGAAGTACATGCGTTTTGGAAGACGGAGTGATCCAGAGCAGCAGGCGTCAGCGGAAGTGGGCGGGGCGGAAGTCCGAGACAGTGTGCTCTCTGCCATGTAGTCACGTGCTCACTGGGAGGAAAGGATGCACGCACAACACGCTCTTTGGACGTATTATTGCAGCCGGCAGCACAACATTTTTA Fifth (short peptide fragments obtained from MS data) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Partial: CCACGTACGGGCGTTAGCGCCAACTTCCTGGCCCCAGGCAGACTCGAAACATGGGAGATAATAGAAAAATCCATTTGGAAAATACTGCTAGTTGTCTCCCATCCTCTCTTTCCGTTAAACCCTATTCCGTGAAGGCATAGTGGGAGAATTGTCAAAATTCTACAGTTTGTCAATATACTGATCCTGAAATCAACCCTCTAGGCCCTTAGTTCGGTTTCTTTTACAGTCTTTCTATCCCTTTTCTATCATAGAGTCTCCCATTTCACTGATGGGCA

96

GCTAAATTTTGTAATTCTGAATTGATTTGAGGGTATTTTTTCGAACATAATATCAATATGACCACTTGGACTCATATAAAAAATTCGTTCTCGATACGCCATGACAAAAAGCTGTAAATGAACTAAATGCAGAGTAGAAAAGAGAAGAGCTAAATTGCGATGCATGTTTCAAGTCGGAGATAAAACAAATCGAGTGCTGGTCAACAGATCTGCCAGCCGTTATCTTAACGGTGCTACTAGGCGTTTACTGGCAACAATGAGCGTGATAGTGTTTATTGTCAGTAGTAGCGTGACGTGAGATGGGGGAGCCGATCAATGTGTTGCTTTATTAATAAGGAAGAGAAAAAAAAGAAGGGGGTGGGTCTGCGTGGGAAGGAAATTTGTGGGAGCAGGCATTTTTGTTTTCGCGCGAACCCATTGGCCAGTGTATAAATCATCGTCTCATTTAAGACAGAAGTGTCTAGAAGCAAGACCGCATTGGTCAGATCTTCGAGGGTGTAATTACTACTGGCTTCCTCTCTTTTCTTGTAAAATGCGTCAAATTAGAAAGCCTCTGAATAAATTCCTTTCGTCCGGTTTGTGATTGCCGTTATTTGCCATTATTACCCGGGGCTTGCCGAGAAGCACGGGAGTTCGACAACGATAAAACCATCGGGCGAGCTGTTGGAGAGAGGGAAACGAACCGCTCGGGGAACGGTCGCAGAACTGTGCGTGAGAAGAAAAAGACGAGCTCGTGGTCACTTTGTGGTCATCTTCATTGTCTGCTGACCCCCACGGAGATTTCATTCGACTCCTCAGACTCATAATAACATCTCCTGTGTCTGACAAACAGGTTAAAGGATCAGTGAAGCTTGAACGAGTGTTTTCTTACCATTTCGTCTCTAGTGACCAAGAGCTCGTAACTTGGCTCAAAGAGTAGACCGGTCTTCTCAATATATAGTATTTAAGTGACTACAGTACAAATTTCACTTTATCTAGACAGCCTTTCCCCCAGAGACCACTGTGATAAAACTATTAGGTCAATTTACTTCAGATCATCTCATTGACGTTAGTCTGGCATTGAAATACTCGCTGTGCTGGATCTATTGCTCGTGTGGTTGCATTTTGGTGCTAGTATTACTCTGCGTCTACGCCACTGAAACAACAGCATGACTTGCGCCAGTTATTTGAGGACTATCCCCGCTCTCGTCGTTGCAGTCCTATGTCTGGGAAGTGCGCTGAGCTCACCGTACAGAGACGAGGACCTGAGACCATTAATTGCGAGCTCGGCCCTTGACTCGGACACAGGTGTCTTCCTGGACATGCGCACACGGCGTGGGAGTGGCCAACAGCTGTATTACTCACCGCATTCTTTCACCAGCAAGAGGATGAATCAGATATCGTGCAGCTGCTGTTCCAATTCCTTCAATAGCAACTGTTGCTTTCAGTGCATGCAGAGGCTTGGAAAGAGAAGCGGGGCAAGATCAGACAACAAAAATGAGGCTCCTGAACTCCTGAGGAACGGACCAGAGCTATATTATAACGAAGGCGGCCGCAGCAGTGACCATGACCTCCTTCCAATGCCCCTTCAACCCGAGGTCAGCGATTGGCGAGCCAAAGGCAGAGCTTGCTCGTGCTGTCTTTATCAGATGCACCAAGAGCCGTTCGCTCAGTCTTGGCCTTGCTGTAGATTGTGCAAGAGATCCAAGCGATCTGTTGGAGCCGTGCCCGCACCAGCTGAGACCAGCAAGAAAGAAACGTGAAGAAAGTCTTGTAGTTGTAGCATTTCAGACTGATTATAATGACTAAATACAACACGAGTAACCTCAATCAGCGCTGATTGGAATAATCAGAATACGAAGACATGTTCTGTATGCCTCCTCCATCATATTATGCTCGAAAATTCATCTATATTTTAGGTGGAAAGTAGAATTGAAAGGACAGTTTA FIRFamide related neuropeptides precursor* Gene Bank Accession Number: n/a Reference: n/a Partial Clone: GAGTAATCTCCCATTTTTCTTACTGTGTGTGTGGTAGATGAGGTCTGTCTCTATCTTCAAAAAACGGTAGATCATTGTCGTGGGGGACGACTTTAAACAAATAGTCATGACGAGTCTTTCTCAGAGAGATGGTTTTAACAGATTCACGAGAAAGTCTGAGCCAGTGGGACAGCGAGAGCGAGCGAGAGAGCGAGAGAGACAGAGAGAGAGAGAGAGAGAGAGAGAGATGGGAGAGAGAGAGAGAGAGAGATGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGACAGAGAGAGAGAGAGACAGAGAGAGAGAGAGAGACAGAGAGAGAGACAGAGAGAGAGAGAGAGAGAGAGAGAAAGACAGACAGACAGACAGTTTAGTTTTAGTAAGGTCTTAAGGTGTTCACAGTTCTGTTTAAGGTCATATGAGCACCAAGGGGTGGATGTCAACAGAGAAGTCCAAACGGGGAATCAGGCTTACTCTGCTCACCTCTCTAGCCTTAACAGCATAAAGTCTGATTTGACTATCAAAGCACCCCGGAAATAGAAATTCACCAGAGTAACAAACATACAAAACAAGAAACGGAAAAACGACGATAGTAAGGATGAGGAAAAGAAAAGCAGCTGCAGTTCCCCCTATGTTGTTCCTGCACAGTATGAGAATGGCATTGATAAATCAGCCCATCTATAATTCTACTCATTCTACTTTGCATAAGAACATGTTAATACCCCCCCCCCCCCCCCCCCCCTTGGTAATGGCCTAAACACAACTTCGGTCAGCAGCACTGCCCTGGTGGGATGGTCCTCTGTGCCACACCGGAACAAACACAGTTTGTGGATATTGAAAAGGAAACAAGCAGTTGCAAACGAGAGCAGGTCGTGTTCTACGAACTGTAAAGCAAAGATTGTTCTGATGAACCAACGTGTGGACCATCATCATCTTCATCATAATTTATAGTAATATGTATTATTATTGTTATTATTATTACTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTACTGTTGTTGTTGTTGTTGCTTTTGTTGTTATAATAATAATACTAATTATTATTAGTATCCTTTGTATTAATATCATCATTATCAAGACAATCTCAGTATCAAAACAATTAATGATGATAACTACTGTAAGTCGTAACGTTATATTTATTACTTGTTTTCAGAAAGTGACGCGTCAGACAAGAGAGACTACCTCCGCTTTGGCCGCAGCACTGACGGCAGTTTCCTGCGCTTTGGCCGAGACGCTCAACAGAACGGTGACAGCACCAGCGATGACGTCAATGACGACCTAAAACGGGACCCCATTATGCGGTTTGGAAGAGGGGATGGTTTCCTCCGATTCGGCAAAGGGGATCCGTTTATCCGGTTCGGGAAAAG

97

AAGAGGAGATCCGTTTATTCGAATCGGGAGAGGCGACCCCTTTATCCGTTTCGGAAAAAGAGATTCGGATGCTAGTGGTGGACAAACAGATAAGCGGCAAGACCCTATCATCCGATTCGGAAGAGGGGATCCGTTTATCCGATTTGGCAGAGGGGACCCCTTTATAAGGTTTGGCAGAGGGGACCCCTTTATCCGGTTTGGAAAGAGGCAGGACCCTTTTATCAGATTCGGCAGGGGAGACCCATTTATCCGATTTGGAAAGAGGCAGGACCCTTTTATCAGATTCGGTAGAGGCGACCCATTTATCAGATTTGGTAGAGGGGACCCCTTCATTCGATTTGGTCGAGGCGATCCATTCATCCGATTTGGCAAAAGAGGAGCAGACAGCGAAGAAGGTGTCGGTGAAGGAGAAGCCTTGTCGAGGTAAAAATAGAAAATAGAAAACATCCTTTATCCGTTTTCTTATAGTACGAACTACATTTTTTTCGTCTTCAAAGATGATCTAAAAGCAGTATGGATGATACAGCTGTGCTTAGGCAATAGACAGTAAACATAGTGCTAGTAGATTGCCAAAGTTATAACTTTCTATACTTTTGATCATGATA FMRFamide5* Gene Bank Accession Number: n/a Reference: n/a Partial Clone: CAAGCGGGGTGGATCAAGCCTTGGAGCTAATTTCGAAGAGGGAGATGATATTGTCAATGATGGGCCTGTCAATAAACGGTTTATGAGGGGATTCCAGAACTTTGTAAACATGGCCAGAAGAAGATACTCGTATCCTCGTGAAGGCGATTCTCAAGATAAAAGGTTCATGCGAGGTCTGTCTGCCTACAAACGTGGTGCAGGAGGGGACTCGTTAGACGAGGACATCAGCAAACGTTTTATCAAGGGGTTAGAGAGATACGCTCTGCCAGCCTCTAAGAGATTCATGCGCGGTTTACAAAACTGGCAGAAACGAAACTCCCTCGAGGGTTCGGGTGCGGACAAGCGATTTATCCGGGGGTTACATCTGTACCATAAACGAGGAGGCGACATGTCTTCTGTGGACAAGCGCTTTATGAGAGGGTTAAATTTGTACGCAAAGAGGTATCTGGGATGGGACATACCGGATTCTAAAAATAGATTTCCTGCCTCAAGCGTTTCAGTTGAGCCTGTCCTAGAGGTGAGCCAGGAAGATGGGCCCTCGTCGGTGTGGGAGGGAGAATACGATCAGCCGAAGAGAGATTATATGTTCACATCAAGTTTGGGGAATTCCTCACCAAAATCTGGTTCAAGGCAGTTCATGAGTTGGAGGGGCGGAGACTACGTGCTTGGCTGACATGACAAATTATCACAGCTCCTTTCAGTTGACGTGTTGAATAACCTGAGTGTAACATGCAAGACAAAGATAGCGGTAAAACTAAGGGGAACATGATAGTGTGATAGTTTATATCATTTTTTTCCTTTCCGATAATATGTTTACAACGTTGTTGTTTTGTTTTGTTTTCTTATTGCTATTTAACGGACTTTTGATACTGCGTTCTAACGTGTTGGTTTTTTTAAAGCAAAGCGTATATTCTAGCTTCTGGTGCTACTGAGTCAAAATCTGTTTCGCTTGTCTTGCGTTTCAAAACCTCTCAGTTTTCTGTCTCTGAGAATTGTCCCTGGTTTTTTTGGGGGGTTTTTTTGTTGTTGTTATATAAAATATCAATCTCTTATTTCACCAGTGTTTCAGGCTCCTATCTGAAAGTTTAAACTTAATGTTATTGCTCTCTATCTACTCTCGATCGAGCAATGTCCAAAAAACGTGAAATTCTATGGCAAACTATATATTCTAAAACAAAATTATACAATATTTGCGTGTTCGTTGTTTTCTCGTTTATCCAGCTTAATTGTGATGAATTAAAGAGGCTTCAGATTAAGTCAATTATAATGTAGGCTTTACATGTGGACTCTTTTATGCCAGATGTGAAGGTTTTAAATGTTCGTTATATTAGTATAAAG Fourth (short peptide fragments obtained from MS data) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: ACGGCATGAACATCGTTAGTGGTTTTTGACGTGCATGTGGGCGTGCCCTGGCTGCTCCAACCTCTGACCCTGTGCCGACATGCTAAAGACAACGCAAAAGTGTTTCACTGACAATGGCATTCCTTCGGAAGAGGTAAATGCCATGATCAAGACTGTTTCAATCGGCAACGTGGCAGGAATGAACTTCACCAACTACTGTGGCGACAGAAAAGATGCCTTCCTGAATGCCAATAAATGTGTTAGGGACGCTGTAAATACGTGTTCTCCAAACAGTTATGATGTTTCTTTAAATTTCGATTACTCGAAATACATGGATGCACTGTGTACTGACAAAAACATCAAACTTGAATGCGTGTCACATTCAGGATTCATAGATGCATTTTCCAAATGTATCAAGGACGAAGCTGCTCGACAATTCAATTCGACCACGGTGAGTGTATGTGATCAAGGAAAAGTCTCTTTGTCTTGCGTAAAGACAGTAGTGGGGGCGTGCGACGAGCATACTGGTGAATTCTACCATGATTTTATGCAGCCCACAGTGGAACAAGCGTGCAAAGGGAATACTGTTCTTGGCTAGATTTGACAGCGTGCGTGGAAGGATCTGAGCACCTAAACTGTGAAGGGAACTAACAACAGACTTTGGCATAATGAAGTTACCCAGTGGAAATCATTGAGGACGTGCATTCTGGTTATTCGAACGCTTGAAGACGCCCTATTTTGAAGAATTATAAAAAGAACAACTTAGCTGTTCCCACATATTAGAGTTTTTTTTTCAGTTCAGATTTCTCTATTTGAAACTTGAATGCTCAAATCCGTGTTACTAGTTTGTGTAAATTAGGTTTGAAATGTGCCATGATATTGAGTCCGCTACGCCTTGCAAACTTTTAAAAACATTTTTTCGGGGTCAAGGGCCGGTTTTCATACTTTAAATGTGTACCAACTGCCAAATATCATTTAAA

98

AAATCTTAATGGGAGTTTTGTTTCGCAAGAAGAGATTCGTTTTGAATTTGGGAATCCAATCAATTTAGTTTGGGTTATTCTGAGCATGCAAAAAAAGTATACATAATATTTGCAGACAATTATAATGTTCAACGATTACTCGTCTAAAGTAATCACTACTGTTCATTATTTTCTTTATTCACGCCGTCTCTCCCTAAAAAATCTGCCTCTACTTTGGAAAGTCGGGCCTTACCTTGAATAACATGACTTCATGACTTCATGACTTCATGACTTCATGAGTTTCATTCGAAACTTACAGTTTACAAAACAAATTTTTGATATTGAAAGAGTGGTGTATGTTGTGAACCAACACAAAATAGGAAACAGATAGGAAAATAATCATGCTGTTTGTTGTCCCTTGCAGTTTGTTCTGCTCTCCTTGTGTCTAATGAAATTGAACGGAAAGATAAAATACTTGTATTAAAACCAAAACATTCGGA Fulicin-like neuropeptide precursor+


Reference: Moroz, L. L., Edwards, J. R. et al. (2006). Neuronal transcriptome of Aplysia: neuronal compartments and circuitry. Cell 127(7), 1453-67. CDS: ATGTGTACCCGACCTGGCCTGGCTGCTCTGCTGGTGCTGATGACGTCTTGTGCCTCCAGCTTCTCACGTGCAGATACCACGAAGACTGGACACCTGAAAAGGCCAACCCGATCAAGTGACCAGTTTTTCGACAGGAGCGACACGGAACCTCTCCAGCCCCCAAACAGTGACCAGTACCTCTCTTCATTGAAAAACAAAGGCCAGTTCAGCAACGAGCAATATCCTGGAGAGTATTTTGACGGGTCCGGGTTCAACGCCGAGGAGGAAGAAAGGCCTCTACATCAAGGGGATGGGGACAGTGTGTCACTTTTGGGAGACAGCCCTCCGAGCCCGGCCATGTCGAAAAGATTTACAGAGTTCCTAGGTAAAAGGGTGCCTTTGGCTAACAGTGGGATGCCTGGCCCTGTGGAAGAGCCACACAAGGAGACCGTAGAGGATAGTCATAGGTCAGAGGGGTTATCACCATCAGAAAGATCGTATAGAGACATGGCAGAAAAGCTGGCTTTACTACTCCGCTCTGACGCCAAAAGACAGTGGGAGTTTGTCGGAAAGCGACCATACGACTTCGTAGGCAAAAGATACGACTTTGTAGGCAAGCGATACGACTTTGTTGGCAAAAGGTACGACTTTTTGGGAAAGAGAAATCCGTACGAGTTTATTGGGAAGCGGTACGACTTTGTGGGAAAACGTCGCCCCTACGACTTTCTAGGGAAAAAGAGTTACGACTTCCTCGGGAAAAGGTACGACTTTTTGGGAAAAAGGAATCCGTATGAATTCGTTGGTAAAAGAACTCCGGCTATGGTTCATGAAGGCGTGATCTCACACAACCTAGACGGCGAGGGTCAAAAACTGGCCATTCCATCCTCTGATGCCCACACCGCAGACAGAAGGTACGCCGAGTTTCTGGGCAAAAGGTCCGAAGAAAGTGGACAAGCAGCTCTGACAGACAGCGCCCGTCTGGCCGCTCTACTCAGCAACACTGGCCTGAGGAAACGGCTGTCCAGAATGCTTCTGAACGGTCAGCTGGCGGAACAGTACCCAGAGTTTATTGGGAAATAG Protein: MCTRPGLAALLVLMTSCASSFSRADTTKTGHLKRPTRSSDQFFDRSDTEPLQPPNSDQYLSSLKNKGQFS NEQYPGEYFDGSGFNAEEEERPLHQGDGDSVSLLGDSPPSPAMSKRFTEFLGKRVPLANSGMPGPVEEPH KETVEDSHRSEGLSPSERSYRDMAEKLALLLRSDAKRQW EFVGKRPYDFVGKRYDFVGKRYDFVGKRYDFLGKRNPYEFIGKRYDFVGKRRPYDFLGKKSYDFLGKRYDFLGKRNPYEFVGKRTPAMVHEGVISHNLDGEGQKLAIPSSDAHTADRRYAEFLGKRSEESGQAALTDSARLAALLSNTGLRKRLSRMLLNGQLAEQYPEFI GK FVRFamide peptide protein 7/Secretogranin-1-0R precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: TCTAGTTCGGTACTTTTTTTTTTTTTTGACTGATAATATCGTGTTGTATTGTTATGAAATAGAATGACATGCTGGGTTGAAATAATGCGGTTTATCACTGGTTTATCCGTTATCCGTATCGGGGGTACGAGTTTCGGTGAGACTGTGCACGAGGGGGAGTGCCCTTCAGGATAAAAGGAATGTGTGTGCTTGGTCTTGGCCGTCCCTGATTTCTTCTGTTGTGGTAAAATAACATTGCTGGCAACCTGCCAATACGCGCTGTGGCATACCTCTTCTTGTTCAGGCTGAGGCGAATATTGAGAGGCGGCAAGTTACTGTTTCGCTTTGAGGTTTGCTTTTCTTCCACTGTGTTCGACTCTGAAGGCTCTGCCGGAGCGTCTATAAAGTCACTCTGTTCTGGAGAGCGTTCAAACCTCCTTGCAGAACTGGGATAGTTTCTATTCTGCGGTTTGCTTTCTGTCTTGAAGTCAGCAAAAGAGCGATCACGTTGATCAAATTGGTGTGGACGTATTTTGTTGTCTTCGTTGTCTGTTTCAGGTCTTCTAGAGGATCTCCCAAATCGAACAAACCTATGATAGTATCTTTTGTCTGCACTCGGGTTTGGTTCTTTATCTACTTGGCTTGAGCGCTCGAAACGATAGTAATTTTTTTCGCCTTCCTCTTGCTTTTCACTCGGGCTTTGGCGCCCAATGC

99

GAACAAAGCTATGCCCTGGAATTGTCCTTTTCCCAAAACGCACGAAGTTGTGTTGAGTTAAGGGAAGCCGTTTTCCGACCTCCTGCATGGCTAGTCGTCTGAGATCCTCTAAATGGTGGTGTTCCAAATACTGGAAGGCGTTTTCGATGTCCTCCCACAGCTGGCCGATGTACTCCACCGTGTC Glycoprotein hormone alpha precursor, CPA2 subunitGene Bank Accession Number: n/a

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Reference: n/a Full Length Clone: CDS: GCGCCCATTCTTCCACAGAGTTTTAGCCATGACGTCATCCTGGCCGGCACCCACCGGACACAATTCAGCCTCTGATATCTCGTCGCCTCGTTCATCATTTGGCAACTGCCGGAGATA CCTGCTTGCTCTGTCTATCGTTGTCCTCTTCTGTCCTTCTGTCACTCCACAAAGACATTCATGGGAGGCCCCTGGGTGTCACTTGGTAGGGCACACTCGTACTGTGTCCATCCCTGGCTGTGTGTCCTTTGAGGTGACCACCAACGCCTGCAGAGGCTTCTGCGTCTCTTACGCCATACCATCGCCTAGTCACACCTTGGCAGTCAACCGGAACTTTGTCATTACCTCCAGGGCTGAGTGCTGTGGGATTGTGGACACCCATGACGTGAAAGTGTGGGTCGCATGCAGAAGTGGGTTCCAGCAGAAGACCTTCAAGTCTGCTCGCAGCTGCCAATGCTCCATCTGCAGACGATCACAGTGACCCCAGGATGACCCCTACACGGCGAAAGTCAAAT Protein: MTSSWPAPTGHNSASDISSPRSSFGNCRRYLLALSIVVLFCPSVTPQRHSW EAPGCHLVGHTRTVSIPGCVSFEVTTNACRGFCVSYAIPSPSHTLAVNRNFVITSRAECCGIVDTHDVKVW VACRSGFQQKTFKSARSCQCSICRRSQ Granulin-like 55 (modulate the growth of cells, is a secreted glycoprotein containing multiple repeats of the granulin/epithelins motif) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: TGCCCCCAAACCTGAGGTAGAGCATGTGGTGTGCCCAGGAGGAACATCTTACTGCCCTGATGGAACCACCTGTTGTCAACTTGCTTCTGGACAATATGGCTGCTGTCCATTGCCATCAG Hyperglycemic hormones isoform A precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: AAAAAGTACCGATGCGAAAAAAAAAAAAGTACCGAAAAAAAAAAAAAAGTACCGACTGCGAAAAAAAAAAAAAGTACCGACTGCGAGCAACTAGTTCCACAAAGCTGAACATGTCTAGACCCTAGACATGTAGACTCTTTGTGGCTGGGGAGGGGGTTAGCGTCCGCTATGCGAACTAAAAAAAAAAGTACCGACTGCGAAAAAAAAAAAGTACCGACTGCGGGAGTAACAGTTCTCACGACAGGTGGTGGCAACGAATGGCTTACGAAAAAAAAAAAAAGTACCGACTGCGNNNNNNNNNNNNNNNNNNNNNCCACGAGGCAAAAGCCTGAAAAAAAAAAAAAGTACCGACTACGAAAAAAAAAAAAAGTACCGACTGGCGAAAAAAAAAAAAAGTACCGACTGCGGAAAAAAAAAAAAA Hypertrehalosaemic neuropeptide Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: CCACTTTTGAAAGAGGCACAGATCTTCTGTACCGACGAGCAAGCACAATGGAATCTTCTAGCATACTTTTGATCCTTGTCGTCCTTGTGATAGGCACGTCCACTTGCCTTGCACAGATTCACTTCTCGCCCGACTGG

100

GGTACAGGGAAGAGGGCGGTCTCCACTGTCACCGAGAAGGAAATCCCACTCTGCTGGCAGATCGCGGACAAAGAAATCATCGATATCATGCTTTTAATACAGCGAACCGCAAAGAAACTCTCTTCCTGCCTGAATACCTGCCCAGAACTGTGACGACATGTGTGAAAGCATTGGCTCAGCTGCGGCAGAGTTGATTTGATGATGCTGATCGCTTGGTCTTTATACGCCTGAGAGCTCTACACTTTCGCCAAAGAACGCCAGAC Hypothetical protein 2 (secretory, pheromone like peptide similar to seductin) Gene Bank Accession Number: AAN83922 Reference: Cummins, S. F., Nichols, A. E. et al. (2004). Characterization of Aplysia enticin and temptin, two novel water-borne protein pheromones that act in concert with attractin to stimulate mate attraction. J Biol Chem. 279(24), 25614-22. Insulin-like 2Gene Bank Accession Number: 19850964

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Reference: Moroz, L. L., Edwards, J. R. et al. (2006). Neuronal transcriptome of Aplysia: neuronal compartments and circuitry. Cell 127(7), 1453-67. CDS: ATGGAACCACAGTCTACCCTCGATTTATGTGTGACCTTGACTCTTCTTGTTTCGACATTCGGAGTAATGACAGCTCAGGGACAACTCGTGTGTGACAGAGACTCAAGGCCACACCCAAGAGGTATCTGCGGCTCTCGTCTGACTCGGGCCCACAACAACCTGTGCTTCCTGTTGAGTCAAACCTACCCCGAGTACTTCCCGGGCAAGAGATCGGCCTCACCAGAGCTACAGGCGTCTCAAAACAAACGTGTTCCCAACCAAAGCCTCATTGACGGATCTACAGCATCCTCTGTCTGTGCTGCCGACCTTGACCTATCTAGGGACGACTGGGCTCGCTCGCCTTCAAGAGACCTTTGGCGGGCCCAGCCCTGCCAGGACCCTGAGGGTAGAGTAAGAGTCCGCGGCGACCCAGAGGCCAGTGCCAAGCTCAATCGGTTCACGGCCATGATGCTAGACTCAATGGCAGGGAGAAGGCTGAAGAGAGGAGGAACTCAGTCCAACATGGTCTGCGACTGCTGCTACCATATGTGCTCACCTAGACAACTCGCCACTTACTGTTAG Protein: MEPQSTLDLCVTLTLLVSTFGVMTAQGQLVCDRDSRPHPRGICGSRLTRAHNNLCFLLSQTYPEYFPGKR SASPELQASQNKRVPNQSLIDGSTASSVCAADLDLSRDDWARSPSRDLW RAQPCQDPEGRVRVRGDPEAS AKLNRFTAMMLDSMAGRRLKRGGTQSNMVCDCCYHMCSPRQLATYC Insulin-like 7Gene Bank Accession Number: 94434859

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Reference: Moroz, L. L., Edwards, J. R. et al. (2006). Neuronal transcriptome of Aplysia: neuronal compartments and circuitry. Cell 127(7), 1453-67. CDS: ATGACAGGAAACCTCGACAGTGGACTGGCATTCACACTACTGCTGGTGACCTCGACGATGACCTTCACACATGGAGAGACTCGATATTGCCGACCTGGATTTTCCAGGCCTCATCCCAGAGGCTTCTGCGGCTCAGCTCTAGCACGTCTGCACGCCAACTTCTGCCTGCTACTCCGCTGGGCCTACCCAGAACACTTTCCCATGGGAAAACGATCTGCTGACCGGGCAGCCCACCATCCGCCCACAACCACTTCCTCGCCCACCATCCACGGCATCCCACTGTCCGCTCTGGCCGATGTGTCA CTTACTGACCACGAGCAGCTCAGAACGATGCTATACCCCGACGCCTCAGGTGAGCTCAAGAAACCATTGCTGAGTGAGCATGAAATTAATGAGGTCTGGATCCAAGACAACGCACCATCTTCTTCCCCTGCAAATCCCTTCGTTCGACGCTTGCGCAAAAGAGCTGCGCGGGGAAAACGTAGTCTTGTTTGTGACTGTTGCTACAGTGCGTGCGATGAGCGGAAACTGGCAGTGTATTGA Protein: MTGNLDSGLAFTLLLVTSTMTFTHGETRYCRPGFSRPHPRGFCGSALARLHANFCLLLRWAYPEHFPMGK RSADRAAHHPPTTTSSPTIHGIPLSALADVSLTDHEQLRTMLYPDASGELKKPLLSEHEINEVWIQDNAP SSSPANPFVRRLRKRAARGKRSLVCDCCYSACDERKLAVY

101

Insulin-like Growth factor 5Gene Bank Accession Number: n/a

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Reference: n/a Partial Clone: GCGGCTCGAGGCCTGTTTGCCTGGTTCTGTCTGCTGAGCAGTCTGTACGTGTCGAGTCTGGCCGAGAGGCGCTTGTGTGGTCAACTGCTGGCCGACACACTGGACATGGTCTGTGAAGACCGAAGGTTTCAACCTCGCCAAGAGGAATGGTCGCAGTGTCGGACAGACGCACGTGAGAGTCCGTGACTTCTCACCTTTGTACAGAATGCTCCACAAAATCGCCCCTGCTCCCCCCAGCGTGCACGGCACCGGGTCATCGACCATCAGCGACCACAATGACGTCACAAGCTTTACTCCTCCTACTACTCGCCCTCAGCCCAAATGGGAGGGTCAAAGGTCGCCGGGAGGTCACGGCGTGAGGTCAACTCTCGTATTGTGGACGAGTGTTGTCTCCGGCCGTGCAACTTCGCCACGCTACAGAGCTACTGCGCCGATCCGGACGATCCCGTGGTGGAAATTCCAGAGGATGTACTGCGGCAGCGCCTCGGTTTCACCACACAGCGAACTGCAGGTCACGTGGGTCAG Insulin-like OGene Bank Accession Number: 94434877

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Reference: Moroz, L. L., Edwards, J. R. et al. (2006). Neuronal transcriptome of Aplysia: neuronal compartments and circuitry. Cell 127(7), 1453-67. CDS: ATGCCTGGCCCGGTGAATAGCCTGTTTGCCTGGTTCTGTCTGCTGAGCAGTCTGTACGTGTCGAGTCTGGCCGAGAGGCGCTTGTGTGGTCAACTGCTGGCCGACACACTGGACATGGTCTGTGAGGACAGAGGCTTCAACCTCGCCAAGAGGAATGGTCGCAGTGTCGGACAGACGCACGTGAGAGTCCGTGACTTCTCACCTTTGTACAGAATGCTCCACAAAATCGCCCCTGCTCCCCCCAGCGTGCACGGCACCGGGTCATCGACCATCAGCGACCACAATGACGTCACAGGCTTGAGGAAAGACAATCTCGGAGGAGCGAGTTTAGCAGACAGCCACGCCCCCGTTGCCTCGGCAGCTGCCTATGATGGCTCCGGGGGTAGCCGCCAGCAGAACGAGGGAGGCGCTCGGCCCGAGCACAAGCTTTACTCCTCCTACTACTCGCCCTCAGCCCAGATGGGAGGGTCAAAGGTCGCCGGGAGGTCACGGCGTGAGGTCAACTCTCGTATTGTGGACGAGTGTTGTCTCCGGCCGTGCAACTTCGCCACGCTACAGAGCTACTGCGCCGATCCGGACGATCCCGTGGTGGAAATTCCAGAGGATGTACTGCGGCAGCGCCTCGGTTTCACCACACAGCGACCTGCAGGTCACGTGGGTCAGACCGGAAGACGGCGCCAGACGACATCGAGGCCCTCGGCGGAAATCAACACCGGTTCAGGTCATCGGCGATTGGGCTCGTTTTTCTGTTGGAGATCGGGAATCAACCTACCCGCAAACCGCTTGTAG Protein: MPGPVNSLFAWFCLLSSLYVSSLAERRLCGQLLADTLDMVCEDRGFNLAKRNGRSVGQTHVRVRDFSPLY RMLHKIAPAPPSVHGTGSSTISDHNDVTGLRKDNLGGASLADSHAPVASAAAYDGSGGSRQQNEGGARPE HKLYSSYYSPSAQMGGSKVAGRSRREVNSRIVDECCLRPCNFATLQSYCADPDDPVVEIPEDVLRQRLGF TTQRPAGHVGQTGRRRQTTSRPSAEINTGSGHRRLGSFFCWRSGINLPANRL Latarcin-4a precursor (secreted by venom glands, antimicrobial and cytolytic) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: GGAGCAGCAGAAATTGAAGAGGAGGAAGAGAAGAAGAAGAAGAAGGAGGAGGAGCAGAGGTTAAGGGAGGAGGAAGAGAAAAGGAAGAAGGAAGAACAGAGGCTGAAAGAAGAGGAACGAGAAGAGGATAAAAGAAGAGCAAAGATTGAAGGAAGAGGCGGAAAAGAAAAAGAAAGAGGAGCAAAGATTGAAAGAAGAGGAGGAAAAGAAGAAAAGGGAAGAGCAACGATTGAAAGAAGAGGAAGACAAGAGAAAGAAAGAGGAACAAAGATTGAAAGAAGAGGAAGACAAGAGAAAGAAAGAAGAACAAAGATTGAAAGAAGAAGCTGAAACGAAAAAGAAGGAAGACCAACGGTTGAAGGAAGAAGAGGAACAGAAGAAAAGGGAAGAACAGAGATTGACAGAAGAAGAAGAACAAAAGAAAACGAAAGAAGAACAAAGATTGAAAGGAGAGGAGGAAACGAAGAAAAGGGA New neuropeptide prohormone (Betsin)*

102

Gene Bank Accession Number: n/a Reference: n/a Full Length Clone: CDS: GAACTTTTGCACTCCCGCGGAAACTGGATCGAGGCAGAAATAGAAGCAGAGAGTCACCCAAAACCATTCACACATGAGCGAAAACTTCCTACCAAACAAAACTATTACGTCATAGCAACTCGGCTGCGGGTATATATAAGCGCGGACGGAATCACGGTCAAACATAGGGCGCTACAGAATTGATTAAAGACGGACTTAAGCTTTGACTAGGTTGTAAGTGCTTGCCTCCCTCCTTGCATTCTCAACAAAAACAACTACAATAACAACGCAAATTCTGCTTTAGACTGAAAAAAAAGTATTCACAGTATAGGAGACCCTCCGTTCCGGCGATCAACAAGAAAACGAACTTCTGAAAACGAACAAGGAAAGGCTCACGTGTTCAGACTGAGGCTACGAAACAGAAACAACATATATACAGAGACGGAGACTGTTAGATTGAACCAGTGAATTGAAAGACCAGGAAGAAGAATATTGAGGCCCTTGCAACGAACGACTTTAGAGCGAAAGAAAAGTTTCTCTGAACTGCAAATTGTAACTTTCGTCCCTCCTGCCAAAAACACTCCTGCACAGTCATGGAACCCCAACTTCTCAGCGTCGTCATTCTCGTTCTCTGCGCATGTGCCGCGTCTGCCCTTCCGACTAACAGCAGACGAGAGGACCTGAGTCATCTGGTGACTCTGCTGGGGAAACTGAAGAACATTGAGCGAGCACGCCAGAATAGCGAGGGGTCAGCACAGGCGCATGCACAAGAGGCATGGGAAGGTCCCAGAGTGTCCAGCTTCCCAGAAACTGAGGTCACAGCTGACGTCATACCAGACGAAGTGTCAGCGGCCAAGGGGTATCTCGGACAGAGGCCGGCGACTGGCGTGGTAAACAAGCGACAGGGGGCGTGGTCTTACGATTACGGTCTGGGCGGTGGCAGGTTCGGGAAGAGAAGCTACGGGGATTACGGTATCGGAGGTGGACGGTTCGGCCGTGACGTGGACCATGTAGATCTGTCGGACGCCACCGAGAATGAGATTACGTCATGAAGGGCGAGCAGGGTCCGAGTGGGTCAAGTAGAAGTGACGGCGTAAGAAGAAATATCTCCATCTTTTTGTGACATCACAAGACACATTTGCTGAGGAATGATGCTTGAAGAGGTCGCTTCTACTTGCAATCGGGTCGTGAATCGTTGAGAACGATGAGATAGGATTAATTAAACAGAAATGAAACCCTTAAAAAAAG Protein: MEPQLLSVVILVLCACAASALPTNSRREDLSHLVTLLGKLKNIERARQNSEGSAQAHAQEAW EGPRVSSFPETEVTADVIPDEVSAAKGYLGQRPATGVVNKRQGAWSYDYGLGGGRFGKRSYGDYGIGGGRFGRDVDHVDLSDATENEITS Leptin precursor 1 Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: ctgaattttggtcttcctaataaaacatctggtaggaccaatacttcagaaaggagcagaatgtcccccaaatgtgcatttctaaacaggaacactttttt Leucokinins precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: GGCTGGGACTCTACCACAAGCGTAGGACAGAAGGGATTTTTTCCATACGTGATCTAGCTTCCCAGTTTTAGAATCATTTCCATTGAGGAACGAATCATTTATCTTGTGGAACCAGGTCCGATTATATTATGTCAGCAGAAAATTCAAATTCATACGAAAACCTCAAAGAATTTGTGTCATTTTGAACCAGAACGGACTGACAACTTGAAATTCTGAATATTTTAAATTATTAGTTACGGCAAAAATAAATTATAGCGCAAAGAAGAAATACGGATTTTGCATGTCAGGACTTTTAAACCCATAACAGATGGTTATGCAAAGGAATTTTATTTCAATTTGAGCACCGCATGTGAGGAAGTTTCAAAACCTGTCAACTCAACATTTCGAAAAAAACAAATGACTTAAAAATCTTAGTACTGCGTGAAAGGAGAAAGTGAAATCATGGAGAAACCATTTTCCATATTGGGTGCTTATAAAGTGTTTTCGAGCAGAAACAGGCCCACTAAGGATAGTTGTTTCCGTTTTCCAAACTCAAATGTGTTGCGGATAGCTACTGTCTTTCTGATTTGTCTACTGGGTTCACAAGACTATGCAGTTTTATGCGAAGTTCAGGACTCGGCAGTCCGTGATTTGACTACATCTGTACACACTTTTACCAAAAGATCCAGTGGGCCTTTAGACTCCTTAGAAATATTGCACTTCGGGAACGACGGGAAGTTTGGAGAATTTTCTGTGAAAAGGTCGGATAACGTTGACCACGAAAACGAAATTGTTGTTGTGAATACAGAAGTGCCAGAGTTTTCGGAAGGTGTTGTTTCTAAAAGCGATGTTCTAGAAAAACTTGCAGACGACTTTCTTGAAGGGGAGGGGACAAATATTATTCCTGAAAA

103

TGAGAGGGATGAGGTAGACGAATTCCCTGATGATTCAGGAACAGAGGATTTGGACAAAAGGGCGGGATTTGCGCCTTGGGCGGGCAAAAGAGACGGAGACGGTGTCGTGGATAAAAGACCAGCATTTCATGCGTGGTCAGGGAAAAGAACACCACATAGCGCTGTAGATGCACAGAAAAGACCCGCGTTTCATGCATGGTCGGGCAAACGTTCAACGTACCCCTACAAAAACAATGAAAAACGACCCGCATTCCATGCCTGGTCGGGCAAAAGATCAGAAAAACGGCCTGCATTTAGCGCTTGGAATGGAAAACAAAAAAAAGTACGGCTGCG LFRFamide precursor* Gene Bank Accession Number: EU886298 Reference: n/a CDS: AAGGTGCGAGCGTCCAGGGTCCAGCTGTCCCAGAATTCCCTGTGTGAGGTCCAGGACAGCACAGCTTAGCCCTGCCCCGCCTGCCGCGCAGTGTGTTCNCCCCATCGTCTGACAGGCCTCAGTTCGAAACCTCGTCCCTGAGCAAGCGGAAGACAAGAGAATGGAACCCAGACCCATCACGCTCAGCCTGGCTGTTCTACTCGCAGCTCTCTGCACAACCTGTGCCACCGGCTCGGCCAAATTAGTCAGCTCAAGCGAAGACAATACCAACAGCCACACTGACGGCCTCACCCGGGACCACCAGCCACAGAAGCGGTCAGCGGCGGCCATCTTGTCCCCAGCCGATGACGCGCTGTTGGTCAGAGCGGACGAGGGAGAGGAGGAGGGAGTGGGAGGAAGCTATCCAGGGATCCCCCTCTCTCTCCCAGGAACCGCCGACAAGAGAAGCACACTCTTCAGGTTCGGCAAACGAGGCGCAGGGACCCTCTTCCGGTTCGGGAAGCGAGGCTCTCTCTTCCGGTTCGGGAAAAGGGGCGGGGCTCTCTTCCGGTTCGGCAAAAGGGGCGGGGCTTTGTTCCGGTTCGGCAAGAGAGGCGTGGCTTCGGGGTCGGGGGCGTTCCCGCTGGCTGACGACTCCGACGTGAAGAGGACGCTCTTCCGCTTCGGCAAACGATCCAACCTTGACCTTCTCAGGGACGTCTTGTCGCAGTACCAGCTCAGCGCCTACCCCGACGCCTACCCCGACGCCTACCCCGACGCCTACATCGATGACGTAGACAGCAAGCGAGGGGTAGACGGGTTTCACTGGGGAGATGACCAATAAGCGAACTGTGTGTGAATAGCCGAGGACAGGGTGACCAATAAACTAACTGTGTGGTGATAAGCTGAGACAGGGGTGACCAATCAACTAACATTGGGGGGACATTAACATTGTGATTAGCGAAGATGAAGGCGACGAATAAACGAACTTTGTACACTTTCAGCAAAGAGTGAGACGACGAATAAAATAAGGAAATTTCACAAGTTCAAAGATGGAGATGACGAATAAACAAACATATTTCACAGGGGCCAAAA Protein: MEPRPITLSLAVLLAALCTTCATGSAKLVSSSEDNTNSHTDGLTRDHQPQKRSAAAILSPADDALLVRADEGEEEGVGGSYPGIPLSLPGTADKRSTLFRFGKRGAGTLFRFGKRGSLFRFGKRGGALFRFGKRGGALFRFGKRGVASGSGAFPLADDSDVKRTLFRFGKRSNLDLLRDVLSQYQLSAYPDAYPDAYPDAYIDDVDSKRGVDGFHW GDDQ Major royal jelly protein* Gene Bank Accession Number: n/a Reference: n/a Partial Clone: GGCTCGAGATTACGTCACACCAGATGGATCGGAAGTCCGGTACGCGTATATTTCTGATACTGGCGATGCGAATCTGGTGGTATTCGATTTTCAGACGAACACGTCATGGGCTTTCAAGGACACGACAATGACACCGGCACCAAACGGTATCTTGACCATTAATGATGTCAACTACACCATGGACTTTCCTATCAACGGAATCGGAATGTCTCCCAATTTCGATTACGTCTACTACTGTGTTATTGGCAACAATAACCTGTACCAGATTCCCACGTCAGTCCTCCGAAACAAAACCGCAGACTTTAAGGAATATGTTAGGAATGTTGGAGACAAAGGTGGGTCCTCTGACGCAATTGTTGTTGGGAAGAAAAATCTGTACTTCGGAGTAATGCAAGAAAATGCTGTTTCCGAGTGGCAACGCAGCAAA GACATCAAGTCCCAAAATGTCTCCGAAGGCGAGGTCAACATGGCCACCATACAAGAGCTGACCTCTAACGACGTGACCATGCAATGGCCGGACGGCTTCACTCTGGACGATAAAGGCTACCTCTGGTTCACCACAGAACGTCTGCAGCTCTACCTGAATGACAAGATGGACTTCTCTGGGGGTCAGGGCTCCAATTTCCGTGTCTTCCGGATGTTCGTTGGTGACGTCAGCTACTTGACAAAACCCACGAGTGGAAAGAAGAGTTGGTGGGAGGAATAGCCTTCTGAAAGATTAAAATAGTATCAACGAAATAGATGAGATTATATTCTAATAGTTTAGTAAGCGT MIP-related peptide precursor Gene Bank Accession Number: 8886135 Reference: Fujisawa, Y., Furukawa, Y. et al. (1999). The Aplysia mytilus inhibitory peptide-related

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peptides: identification, cloning, processing, distribution, and action. J Neurosci. 19(21), 9618-34.

Myomodulin-like Neuropeptides Precursor 3* Gene Bank Accession Number: EU934739 Reference: n/a CDS: AGTCAGGGAATCGTTTGCATGGCGAGACGCGGACTATAGAAGGAGATGGCAATCTGTGTTTCACATCAACACAAACACAGGAGGAAAACCATTAAAAGATCAAACATGGCGCAAGCATAAAATGAGATGTCGCAGTACCCTATCACACATAATTGTATAGAATAGGCTTTTTGAAAAGATAAATATACCCAAACATGGCGAAGCTGGACACAAGACGTGGCAGAAAAACAACAGCAGAGAATTGTACAAAAAAGTCCTATCATCCACTTTGTGACGTCATGGGGAGTGTGTGAGACGTGAGAGATCCGCCTCCTGTAACTCGTGTTAGGTTTCAGTCATGCGACAATGGCGACGGTCACGTTTTGCTTGAGGCTGAAAGCTTCCTAGGAACACCCGGGATTCGTTCCCTGTTTCCTTGATCTCTCTACACTAAACCACATTAGACCTGTCAGTATTCTGTTAACATATTGGAAGCTTACCATTCCTTTATTTCTTTCTAGAAAAATGAAGTATTGGTGTCAATGCGATTTTAAAATGTCCACTAACTTTTAACCCTAACCCTCATGGTAAGAGGATATAATAATTCTATCGTTCAAAGATAACCACTTCCTCCATGTTTTTTTAAGGGGGATGTCATATTATATGATGTGTGTCACTTTGAAAACTGTTTAAACTCCCGAAGGTATTACAGATAAATTTGACTTTTGATTGAAGATGTTGTTATTTTTTCTTTCAGAGCAGTTTGTATTTTCGCATTTCGGCAGAGGTAACCATGAACATGACCAATCTCCACTTGCATTGTACAAGTACGACTAAGAAAGAAAAATGCTCTTTATACGCCTATTGCACCGTACACGGCTCCTACGGTATACGCTGAATGTGGCAAAATGGTTTTTGGCTCCATCATAATACAAAAAGTTAACGTCCTGAGTCTTTCTTCAATCCCCCTCTTTGGGTTAGTTGGCTACATTAAGCTGACAGACTTTCATAAGCAAAGCCACTGGAAAAAAATAAACAAACAAATCCACCCCCCAACCCCCCCCCCCCCCAACAAAACAACAAGAACAATTTCAGTTAGGACTTGAAAGAACAAAAGGGTAATAGTGAAACATTGTTTCGAGGATTCAGGGAGTAATTCGATTTTATCGGCTTTGGTAACAGTAGCGTCGTGAGCTCTTCTTGGTTTTACAAAAAAAAAAAAAAAAAAAAAAAAAGAGGACAGGGCTCGGGATCTGGGGCACGGAGAGTGAGAGAAACACAATGTGCTTTGGGGAAAAAAGCACGTAAGTCCCACTCGTCCCCAGACACGAAGGAGCAAACAGACATCTGGATGGGCGTGGCCACGATCTGTGGTGACATCACGGCTGGCGGCAATTAAAACTTTGGAGACATTGACTGGACGCAAGCAAAGCTGGAACCAAATTATGTCAACTTTTCAAAGGAACTGACGAAGGTGTCCATGTTCTATCGGATCACGTTTGACTACACGTCTGCTTCCTCTACTCTGCCTTCACGTTTTATTTAATCGTCTAAGGAACGCGGGGGAAGGCAAAGTCGATTTCAAAAACGTAACTCAAGCTTGTTAACACCTGACCCCTCTTCGTCCCCTCTCCCTGATATAATTTAGTAGCCTAGGACAGACAAAGTCAACACACATATCTCAGTGGCATTTGGAATTAAAGGGTTTTCCGAGGAGTGTCTTTATCTCAGATCTTAAGGACAAACGACAGGAAATGTGAAACACAAAAGGGAAGCAACACACGTGCGAAAGGAAAAGATAAATATGTGAATTGAAAAATACACTGGCGAGGAGCAAAGTTCAATAATGCTCTGAAAAAGAAACGACAGCCGGGGGCTGATCTTGTTAAAAGACAAACAAACCCGAAACACAAGAAGAGAGGAGAGGAAAAGAGCTGACTAAATGACTAAAACCAAATTAAAACAAAGGTGAGAAGAGGAGAAGATTTGACGATTTCGTTAAATAGACCAATACATTTTTCATTTTTGAAACACATTGTACGTTTTATCAGCGTGACCTAGCAGCTGACCCCACTCCCTTTACTAATCGTTTTCTTGTTGAACTTTGGCAGTTTTATTTCCGCTGAGACACAATAATGTTTGCGTTATCCTCCCAAAGACGGCATACATTTTCGTGCAGCAATAATATTCAAGGTTCTTTAAAAGCACAAATGAAAGAGGATATTGAGGCGTACATTTGTCTTGAAATATAAAGTCGAATTTTAGAATGAGAAAAAGAGCGAGAGAGAGAGAGACGAGAAGAGAAAAAGCGTACAAGCGGTAAATTTTATTGATTTTACAATGTAATAATGCTTTTCCCCAGACTCAAGAAAAAACAAACAGACAAATAACAACAAACGAATGCTTTGACAACTAAAATAACATTTCCTGAAACTCAACAACAAAGCTAAATGCACCCCACACCCCCAAACCCCCCTTCCTTTGCAATCACAAAAAGAAAGTGACACAAAACAAAGCAAAAAGCACACGCGACAAGACAGCATTAGGAGAAAACGAGCGAAAGCATTACCAGGGAAATAATTTTGTTTCTGTTTCATCACAGATTAACTATTTGTAACACGAGGGAAAATTTGAAAAGATTCCTTCCCTCACCTCACCCGTCCCCTTCCCGGAGCTCTCCATTTCGTCTTCATAACATGATAGCATGATAGTTAATATTATTTTTTCCCTCCTTTTTTGTCCCCACCACACACCTTATGAACATAGGTACCATTTATAACAGGAGAATCTTTGATCTCCTCTTAGACTGCAGCAGGAAAGGCAAATCGAGAAAAAAAAATCACTTTCTCCGTTTCCGTTTTTATATTTTTATACATGTACATGTAATTCTTTTTGTTTCAATAGCATTTCAACCCTCCTCTTTCTTAAATAAAGCACGAATAATTTTGAAAATAACATAATAGCGCATGTTTCTTATTCGCGGGCCTTTAGTAAGTACCGTAATAGAATATAACAGCAGCGTGTATTCTTGTGAATGGTCTTTGAACCTTTATGTTTACTCAGGGAGTGTCACTCTTTCTTTACACCTTCGTATTCTACAAGACTGAAACAAACGGGATTGTTTAAAAATGCTTTATCGGGCTCTCATTATGAAACGTCAGTTCAGGATGTTCGATCTTGAGACACCAAGTCTCTACACTCCTCTTTGCACCCTCCCCCTGCCCCC

105

AAATATAAAAAAAAAAATCCCCGCTTCCACTCACTCCCCCAAAACAAGAAAAGAATCTGTGGAACACCTTCTTTCTTCAATCTGCCCCAGCTTAAAACGGCTCCACCTCCACACGTTTCATCCAACATCCCTTCAGTTTTTGGTCCATGTTCGCATCCTTCCTCCTCATTTCGACCTCCCAAGGACATCTCCAGATCTTGAGTACGTGGAACCGAGCTCAATTTAAAATGAAGAGATGGGGGTATTGGACAAGACCAAAATAGCAGTATCGCATGGACAAGTTCAATGAGGTGGAAAAAAAACACACCGACAGGAAGATTAAATGAGTGTCAAATCTGTGCGGCGTTACTTGTCCTTTGCGCGGCGTTCTGTTTTTTGTCAATACGTCAATGTACTAAGACCGCTTCTGACGTTGAATTTGATGAGGTCATTTCTTTCTGTGTCCAGGTTTTTGTGCCTATTAGGTGGGTTCCAGGGCAGGGGATGGGCGTGGTCACAAGCACGTTTATACGTAATGTGTACGTCTATAGTGTGGTGCTGCCGAAAAAACGGTTTTATACACTTGTACTCTAAGAACAAGGGAAACAGTATTGGCAGGCTCGTTGAGGATTTTGGAAAGTTTATTAAAGTTGTGAGGATGTCTGTACATTTCGACTTATCCAGATCATCAGGTGGTATGCTGAAGTGAATTTTATCACCACAGTAATAACTCACTGAGTGTTACGTTGTGTGCGTGTAGAAATGTGAGTGTGTGTTTGTGTCGCAAGCTCTTGTATCCAGACTAGCCTACATATTGCATTATTTAGAAAGAAGCGAGCTGATATTATGTCGACGGTAAATGTATGTTCACTAATGTGTTTACAAGCACGTTTATACGCAAACATATGTCCATATTCTGATACTTCTAAAGGAACCAGAAACAATTAATTTATTTGCAGGTAACGAGGCCGCCAGATATAGCTCGGCGTGTTTCATTAAAAAGTGTTATTTTTAGAAGTTAAAAAGGGAAAACCTTGTTTCCCGCGCACCACTCTTACAGCCTCAAGTTTCTCCCCCTATCACCTGATGCCTTTTTAAAGTTGAACTCCAGGGCGGCCGGATCTGCTGCTCGGCATCCCAAGAGATGCCATATGTCACGAGGGGATGTAGACATTACCGAAACGGCACACACTGACTTATTGCTGCAGGAACATACCGACTCCTCATTTCATAGAGACTATTTTAGATAATATAAAACTGACAGGGATTAAAAGCGGTAAATAGGTTTTGAGGAACAGAGCAGGGGTTACTACTGATAACATTGCACGAATGTAGAAGACCACAAGTTATGCATTATTTTCTTTAATATAATATTTGCACTATAATACACCCTTTTCGTCCTCTAAACAGCATTGGAAGTTGTACATAATCATTTACAGACTGGATTCTGTCATGATGGAATTCAAAATGTGGTGACAGAGCATAAGATCTTGTTTACTTGAGATGTTTGTACAAATACAAGAAAGAGCAAAGACGTGGACAGTGAGTAAAACATTGACAACATTATATGCGCTTCTTCTGTTGATGCTAATGAATTTGTAAACATCCCTCTCACCACACCCTCTTTTCACCCCCAACCAACCCATTTTCAGAGTCTTAATTGGTAACATCAATAATCAAAAAGACGTATCGAGCAAGCGTTGGGATTCTTGTGTACTGTGTAGGACGTA GGAAGCAGTTACTTCCCTATCCGGACAAAAGAGGATTGTCCGCCCCTGGATCCCCGCCGGTACCCTGCCCTGCGCCCGAGAGCGGGCTCCACCATGACGTCAGAGTTCTTGCCGATGCGGACAAAAGCGGAGGATGGGATTTTCCCTATTCTGACAAACGCATGCGCTCTGCTCGCCACGCTGATCGGCTTATCGTCATCAAAAAGAACACCGTCCCCTAGGCTTTCTTCAGACGATGATGAGTCGGAATGTGAAGATGAAGAAGATGAGGGCGAGGATAGAGAAGACATTGAGGATGACGACCCAACGGACGACGGTTGTGCCGGTGCCCCTGGAGATGACAAAGCTTTGCCGATTCGCACGAAGTTTGAGGCCCTCTTGTCGGAAGACAGTGCACCTTCGCCATCAGCTGCGTTGTCCATGCCCGATTTCCCGATCCGGACAAAGCTTGAGGCTCGCTTCTGACCGTCGTCCGTGCCGTCTACGCTGAGATGAGGGCCGGAATTGTCCCCGGACTTTCCAATTCTGACGAAGCTGGACGCTCTCTTATCCTCCCCGTCACCGGACTTTCCGATTCTGACAAAACTAGAGGCGCGTTTGAGCATGTCTTGGCTGTTGAAATCTGCGGGGCTGAGATCATCATAGCCGAGAGAGCTTCCAAGATCGTACCCGATGTTTTTGCCAATACGGACGAAACTGCTGGCTCTCTTTTCCGCAAAATCTTCGTCATCAGGCAATTCTGACTTGCCGATGCGGACAAAACTGGAA GGGTTGCGGCCAATACGAACAAAGCTCGAAGGATTTCTGCCAATTCTCACAAAATTTGAGGGAGCCTTGCCGATTCTGACGAAGTGAGATGCGCGTTTGATGGCGTCTTTGAGCGAGGCCAGTTGGTCAACGGGGGTTTTCCCGATCCTCACAAAACTAGACGCGCGTTTGTTGTCGTACTGGTCCTCACTGGCGTCATCGTAAACCTCGTCACCTGATCTGCCAATACGGACGAAGCTGGACGGCCGTTTGCCGATTCTGACGAAGTGAGACTTTCGTTTTTCTGCTTCGGGCTCGTCCCCATACATCCGGTTATAGTGGTCATCTACGTAACTTCCGTAGTCAGACATCCTGCCAATTCGGATGAATCTGTGTAGAGGTAGGTTGCTTCCTCGTCCTATCCTCACAAAGCTAGACGCCTTCTTTTCCATCTCATCAGCGTAAGGGTCGTCTCTCAGATAGTCCTGCTGGCCAGCGTCGTTCTCGTCAAAGCTCCCGTCTCCTCCAACATCTTTCCCGATCCGGACAAAACTGCTAGGGCGACCGATCCGCACGAAGTTGCTCGCCCGCTTCTCTCGAGACTCAGCCGAGTCCGTGACGTCAGCCGGACTCGTGGCCTTGACCGTTGAAGCGTCGTCCTCAGCGAGGATTGCAGACGGGCAGAAAGACATGAAGCAGACGACACACAGGAGTAACGGTATCGCCATGGCCCTGTAGGACAAGGTCGGTCGAACGGCAGCCATCTTGGTATCACTTACCGCTTAGGCTGGACTCAGGGTCTCGGAGTACTTGATTCTATGTGAGAGATTTGACGAGATTTGGTTCTGAGGAAATACAAAACAAAAATAAAATAGAAGGATGTGTGGAGTGGTAGCTTTCGGGGAGTTAGAGTGC Protein: MAAVRPTLSYRAMAIPLLLCVVCFMSFCPSAILAEDDASTVKATSPADVTDSAESREKRASNFVRIGRPSSFVRIGKDVGGDGSFDENDAGQQDYLRDDPYADEMEKKASSFVRIGRGSNLPLHRFIRIGRMSDYGSYVDDHYNRMYGDEPEAEKRKSHFVRIGKRPSSFVRIGRSGDEVYDDASEDQYDNKRASSFVRIGKTPVDQLASLKDAIKRASHFVRIGKAPSNFVRIGRNPSSFVRIGRNPSSFVRIGKSELPDDEDFAEKRASSFVRIGKNIGYDLGSSLGYDDLSPADFNSQDMLKRASSFVRIGKSGDGEDKRASSFVRIGKSGDNSGPHLSVDGTDDGQKRASSFVRI

106

GKSGMDNAADGEGALSSDKRASNFVRIGKALSSPGAPAQPSSVGSSSSMSSLSSPSSSSSSHSDSSSSEESLGDGVLFDDDKPISVASRAHAFVRIGKIPSSAFVRIGKNSDVMVEPALGRRAGYRRGSRGGQSSFVRIGK Neuropeptide CP2 precursor Gene Bank Accession Number: 18844703 Reference: Vilim, F. S., Alexeeva, V. et al. (2001). Cloning, expression and processing of the CP2 neuropeptide precursor of Aplysia. Peptides 22(12), 2027-38. Neuropeptide Y mRNA, complete cds Gene Bank Accession Number: 155793 Reference: Rajpara, S. M., Garcia, P. D. et al. (1992). Identification and molecular cloning of a neuropeptide Y homolog that produces prolonged inhibition in Aplysia neurons. Neuron 9(3), 505-13. Neurotoxic peptide caeron-like precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: CGCAGTCGGTACTTTTTTTTTTTGAAGAGTTTGCAGATATGACACTTTATTTCCGCGCTCAGTTCATCATCTCTCGCGACGGACTCATCTAATTCCGCCATTGGTACTGTCGCTTGACGGACTCATTGAGCAGATTCTAGCGTCATGAAGAATGTCGCGTGACTGACTCGTCTAGTAAATGATGTGAGGGTTAATGTCGAAACGTTGCTCACATGCGCTCTTGACGCGACAGGTGCCCTTGAAAATAGGGAGGTTGAAGTCTTGTCTTTTACTGACTGCCAGGTTGAAGTAACAGCATTGGTCTTTTCCACAGTCGCTGTTGCTAGTACAGGTCGTGGCGGCCTTGCGTTTGACGACCACCGGTCTCTGACAGGTGCCCATTTGACTGAAAGGCGTGTCCGAATCCTTCACAAGCTGGCACTTGAGACCTTTTTGGCAGTTGCAGGAACTGAACAAGTTGCAGGACTCTCCCTCAGCGCTGAGTTTCTGGCAAAATCCGGGGATACCGTCCAGGTTGAGCGCTCTTCTGCTGGGCGTGACGCGCATGCAGCACTCACCCTTCCCGCACTGGTCTTCACGCACACAGTTGTCACCCTGGGCCGCAAAGGCCAGACCGAGGCCCAGAGCCAAGACAGCGATAGT Neurotoxin-like-1Gene Bank Accession Number: 71148939

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Reference: Moroz, L. L., Edwards, J. R. et al. (2006). Neuronal transcriptome of Aplysia: neuronal compartments and circuitry. Cell 127(7), 1453-67. CDS: ATGATGAAGGTTCTCCTGTGTGTGTCTGCTCTCGTCCTCGTCAGCGTGAACGCAAAGGTGGGTGACGAGTGTTGGAACGAAAGCACGTGCGACCCTGGAGAATGTTGTTTGAAGAACGAGGGCTCTATGATCGTCAGCAAGAAGAGATCTGACTTTGGACCCATCTTGGGAGCAACGAAA GGGACGTGCCAAAACTATCGGTTGGAAGGCGACAACTGCCAGAGAGAAGACATTTGGGACGAAGTAACAATGTGTCCCTGTGAGACCGGCTTGAGGTGTGTCTCGAGGGAAGAGCCGAGATTCGTTCCAGGATTCTTTTCAAAGAGAGTTATTATAGCGCCTGCCAAACCAGGATACATTTGGCGGGAAAGGTGTGAGAGAATCGAGAGAAACCAGGATTTTTTCGTCAGCTAA Protein: MMKVLLCVSALVLVSVNAKVGDECW NESTCDPGECCLKNEGSMIVSKKRSDFGPILGATKGTCQNYRLEGDNCQREDIWDEVTMCPCETGLRCVSREEPRFVPGFFSKRVIIAPAKPGYIW RERCERIERNQDFFVS Neurotoxin-like 2Gene Bank Accession Number: 71148941

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107

Reference: Moroz, L. L., Edwards, J. R. et al. (2006). Neuronal transcriptome of Aplysia: neuronal compartments and circuitry. Cell 127(7), 1453-67. CDS: ATGAAGGTTCTCCTGTGTGTGTCTGCTCTCGTCCTCGTCAGCGTGAACGCAAAGGTGGGTGACGTGTGTCAGGACGAAAGCACGTGTGACCCTGGAGAATGTTGTTTGGAGCCCGTGGACTCTTTGATCGTCAGCAAGAAGAGATTTGACTTTGGCCCCATCTTGGAAGCAAGGAACGCGACCTGCCAAAACTATCTGTTGGAAGGCGCCGACTGCGAGAGCCAAGACATTTGGGATGGAGTGACCTGTCCTTGTGAGACCGGCTCTATGTGTATCTCAAGACAAAAGCAGAAATTCTTTTCAGGTTTCTTTGCAAGTAGAGCATTGCCGCCATCCCAACCAGGATACACTTCGCAGACTAAGTGTGAGAGAAACAACGAGCCCATACCCAGCTAA Protein: MKVLLCVSALVLVSVNAKVGDVCQDESTCDPGECCLEPVDSLIVSKKRFDFGPILEARNATCQNYLLEGA DCESQDIWDGVTCPCETGSMCISRQKQKFFSGFFASRALPPSQPGYTSQTKCERNNEPIPS Orcokinin peptides type A precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: TAACAGAGATGAGATCA GAGACAGCAGACGTGACATGACCAGGAACAGAGACAACAGATTTGAAACGAAAGAGATCATGCGCAACAGGCGGGGCTTGGACGAGATAAGCAGAGACAACAGGCGTGACATGAATGAGATCAGCAGAGACAACAGTCGTGATATGGACGAGATCAAAAGCAACAGACGTGAAGTGAACAAAGTAAATAACGACGAAATCCGAGACAACATGCGTGTGATGATCGGTAACAGGGACAACAGGCATGACCTGAATGA GATAAGCAGAGCAAACAGTCGTGACTTGAACGAGATCAGAGACGACAGACATGAAGTGAACAGGGTAAGCATAGATGAGATCAGATACAACAGACGTGACATGAACGAGATTAACAGAGACAACAGGCGTGACATGGACGAGCTCAGAAACTACAGGCGTGACCGGAGCGAGATCAGAGACAACAGGCGTCACATGAACGAGGTTAACAGAGACAACAGGCGTGACA Orexigenic neuropeptide QRFP precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: CACATCGTTTGTCGGTTTGGGGGTTTGGACAGAGCGAGGTGTGTGAGGGACACAGGTGTCAGGTC Pedal peptide 1Gene Bank Accession Number: 56200042

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Reference: Moroz, L. L., Edwards, J. R. et al. (2006). Neuronal transcriptome of Aplysia: neuronal compartments and circuitry. Cell 127(7), 1453-67. CDS: ATGATGAGACAACTGTTCGTCCTGGTCATCGTGGCCGGAGCCGTGCTCGCCGACAACTACTGGTGCCCCAAGGCCGGCGAGGCTTTCGAGTGCTTCGAGTCGTCCCCCACAGAAAGGTTTTGCCTGACCAACGGACGCGAGACCGCTGTCATCTGCTCCAAGTGCAGGAAGAAGTTCGACTTCTGCCGTAACGACTTCGTCAAATCCAAGAGAGCCCACACTGACTGTGGAGCCGGTTGGGAAAGCACTCCCTGCACCCACGACAACTCACACGTGCCCGCTGTCTTCCCCGGGAAACTTTAA Protein: MMRQLFVLVIVAGAVLADNYW CPKAGEAFECFESSPTERFCLTNGRETAVICSKCRKKFDFCRNDFVKSK RAHTDCGAGW ESTPCTHDNSHVPAVFPGKL

108

Pedal peptide 2Gene Bank Accession Number: 94434888

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Reference: Moroz, L. L., Edwards, J. R. et al. (2006). Neuronal transcriptome of Aplysia: neuronal compartments and circuitry. Cell 127(7), 1453-67. CDS: ATGGCCAAAGTCGGAGTGAGCAGTCTCTGCCTTGTCCTTATCCTTCTCTGCTCTGCCGTGAGCGGAGGTGAAGTTCCAGATGGAGTTCTCAAGAAAAAATCACAATTTGTCCAAGCCTCCTCCGTGAGTGAGACAGCCGAGGGTGCAGGTGATGGTTCTCTCAGTAAACGACATGTGGGTTCCATCGGAAGTTCTTTTATCAAACGACCTGTCGATTCTATTGGAAGCTCTTTTATCAAACGACCAGTCGATTCCATCGGAAGCTCTTTCATCAAACGACCAGTCGATTCCATCGGAAGCTCTTTCATCAAACGGCCAGTCGATTCCATTGGAAGTTCGTTCATCAAACGACCAGTCGACTCCATTGGAAGTTCCTTCATCAAACGGCCAGTTGACTCCATTGGAAGTTCCTTCATCAAACGGCCAGTTGACTCCATTGGAAGTTCTTTCATCAAACGGCCAGTCGATTCCATTGGAAGCTCTTTCATCAAACGGCCAGTCGATTCCATTGGAAGCTCTTTCATCAAACGACCCGTCGATTCTATAGGAAGCTCTTTCATCAAACGGCCAGTCGATTCCATTGGAAGTTCGTTCATCAAACGGCCAGTCGATTCTATTGGAAGTTCGTTCATCAAACGGCCAGTCGATTCCATTGGAAGTTCGTTCATCAAACGGCCAGTCGATTCTATTGGAAGTTCTTTCATCAAACGGCCAGTCGATTCTATCGGAAGTTCTTTTATCAAACGGCCAGTTGATTCCATAGGAAGCTCCTTCATCAAGCGACCTGTTGATTCAATCGGAAGCTCCTTTATCAAGCGACCGGTTGACTCTATCGGAAGCTCTTTCATCAAACGTGGCGTTGATTCAATTGGAAGTTCTTTCATTAAAAGACCTATTGACACTATTGGCAGTTCGTTTATAAAGCGCGGGGTGGATTCGATTGGTAGTTCTTTCATCAAACGGCCGGTGGACAGTATTGGTAGCAGCTTCATCAAAAAGAGAAGATTCGGGGGCGAAAATGAATTTATGAAGAGACCAATCGATACTATTGGAAGCAGTTTCATCAAAAAGAATGTCGACTCTATCGGTAGCAGCTTTATCAAACGGCCCATTGACACGATTGGAAGCAGTTTTATCAAGAAGAATATGGACCAGGCAGCGTTTATTAAGAGGCCTATTGACACTATCGGAAGTAGTTTCATCAAGAAAAACATTGATTCAATTGGCAGTAGCTTTATAAAGCGACCCGTTGACAGTATCGGAAGCAGTTTCATCAAGAAAAACATTGATTCTATTGGCAGTAGCTTCATAAAGCGGCCGATAGACACCATTGGAAGCAGTTTCATTAAGAAAAGTTATGGACAAAACGAAATGATGAAGCGACCTGTGGACTCGATCGGCAGCAGCTTCATCAAACGACCGATAGACACCATCGGGAGCTCTTTCATCAAGCGAGGGGCTTTCGTTTACGGGGGACGCCATTTCAGGAAAAGGCCAAAACCCACTTGGCACAGGTTCATGAAGAGGCCAATCGACACCATAGGAAGTAGTTTTATCAAGAAGCGAGATGTGTTGGAGGAGGAGGCGGCGATGGACGGCACACAGGGCAAATTAGGCGCGAGTGATTCCGAAGGTCAGAGCAGACGAAAGCGTAGCACGAGCTTCCACGTGTTGGACTCACTTCCCCTGAGACGTGTGATCAACAGCCTGTACACGTCAGATCTTGATGGGTCACGCGACGAGCCTGCCCATGAGGGCCAGCTCACGTACGGCGACGTAGTGGAGCTCCTCCGATTCTTGGACAGGAACGAGTCTGTCATCAGTGATGAGAAGTTCGGAGACATTCTGGACAAGCCATACCTGGGCTCCGGTCAGATGGGGAGGTAA Protein: MAKVGVSSLCLVLILLCSAVSGGEVPDGVLKKKSQFVQASSVSETAEGAGDGSLSKRHVGSIGSSFIKRP VDSIGSSFIKRPVDSIGSSFIKRPVDSIGSSFIKRPVDSIGSSFIKRPVDSIGSSFIKRPVDSIGSSFIKRPVDSIGSSFIKRPVDSIGSSFIKRPVDSIGSSFIKRPVDSIGSSFIKRPVDSIGSSFIKRPVDSIGSSFIKRPVDSIGSSFIKRPVDSIGSSFIKRPVDSIGSSFIKRPVDSIGSSFIKRPVDSIGSSFIKRPVDSIGSSFIKRGVDSIGSSFIKRPIDTIGSSFIKRGVDSIGSSFIKRPVDSIGSSFIKKRRFGGENEFMKRPIDTIGSSFIKKNVDSIGSSFIKRPIDTIGSSFIKKNMDQAAFIKRPIDTIGSSFIKKNIDSIGSSFIKRPVDSIGSSFIKKNIDSIGSSFIKRPIDTIGSSFIKKSYGQNEMMKRPVDSIGSSFIKRPIDTIGSSFIKRGAFVYGGRHFRKRPKPTWHRFMKRPIDTIGSSFIKKRDVLEEEAAMDGTQGKLGASDSEGQSRRKRSTSFHVLDSLPLRRVINSLYTSDLDGSRDEPAHEGQLTYGDVVELLRFLDRNESVISDEKFGDILDKPYLGSGQMGR Pedal peptide 4+


Reference: Moroz, L. L., Edwards, J. R. et al. (2006). Neuronal transcriptome of Aplysia: neuronal compartments and circuitry. Cell 127(7), 1453-67. CDS: ATGAATCAAAACTATTTGTCCAAAAGACCGCTGGATTCCATATCTGGTAGCAAATTTCACGGAATGAATCAAAACTATTTGTCAAAACGACCGCTGGATTCCATATCTAGCGGGTTCAACGGAATGAGACAGAACT

109

TCTTATCAAAGAAAAGCTTTGACTCTATTTCCGGCAGCGGGCTGCACGGGATGGGCCAGAACTTTCTCGACGGGAAGAGAACATTTGACTCAATCGGAGGGGGTGACTTACAAGGAATGGGCCAAAATTTTCTGACATCAAAAAGAGGTTTTGACCCAATTTCAACAGGCTCTCTGCACGGAATGCGTCAAAACTTTTTCGGACACAAAAGAAGTTTTGACTCAATAAGCAGAAATCGGTTAGGAAATTTACGTCAAAATTTTTTGAGTAAACGAGCAGACGAGAGCGCGGATGAGTGGAGTGTTAATGACGCTTCACCCGAGAAAAGATTCGATTCGATCAGCACTAGCGCTCTCAATGGCATGGGCCAAAATTTCTTCGACAAAAAGTACTTTGACAATTTTCCAGAAGGTGCTGTATCGAAGAGGTTCGACCCCATTTCTTCATCGGGGACCATAAGCGGATTACAACAGAATTTCCTTAAGAAAAAAGACTCTGACTCCAGGTCGGCGGGCGGGATGGGAGGAGTCCGCCTGAGTTTTCCACGACAGACAGGAAGTGTGGACGGCATGACGTCATCTAAACGCCAGTTTGACTCCATCTCTGGGGGCAGCGGCATCGGCGGTCTGGGTCAGAACTTCCTGAACAGGCGACAGTTTGATTCGATAAACGAGGGGGATATAGGCGGGATGGAGCAAAACTTTCTCAGTGGGAAACGATTCGATACCATTTCCAGTGGTAAAATGGCAGGATTCAATCAGAATTTTCTTGGCAAGCGACAGTTTGATTCCATTTCTACTGGGGAGATGAGCGGGATGGACCAAAACTTTCTGGGAAAACGCGGCTTCGATTCCATCAACAGCGACTCTCGCATTTCCGGAATGGGGCAAAAGTATTTGGTTCGTCGTAGCTCCAGCCTCGAACCTCTACCCTCAGCAGAAGTGAGCGAGCAGCCCCATGACGTCTACAAACGCAGCCCAGAGGATGTGACGTCTCTGGAAGCCAGTCACGACTCGGCTGTTGTCACCGGCGATACTTCAGAACCGATCGGACCTGAGCATCGTTTTATAAAACGCAGCTTCGATTCTATTTCCAGATTTTCAGGAGGTATGGCGGGCATGAGTCAGAACTATCTGTCTCGCAGAGCGTCTAACACTCGTGCTCTGGACTCGAACTCCCAACCGTCGTCGTCATCAGCAAGCTACACTAGCCGTGGCACTAACGACGTGACTTCACTCGCCGGAGTAGACAACGATCACGAACTCTGGCGCATCCTGGACAAGAAAAACTTGGACTCAATCTCCAGATTGGGCGCTTATTTCCGGCTTTCATCAGAACTTCTTAGGCTTGCCGTCTGCCAAACGCGCGGGCCAGGGCGCGCGAGACTCCAGCCAACATCACCCCGCCACCGATCCTCTCTTCAATCGATGGGATTACCAAAGATCGGGGTCCGCAGATTTCTCAGAGATTAA Protein MNQNYLSKRPLDSISGSKFHGMNQNYLSKRPLDSISSGFNGMRQNFLSKKSFDSISGSGLHGMGQNFLDG KRTFDSIGGGDLQGMGQNFLTSKRGFDPISTGSLHGMRQNFFGHKRSFDSISRNRLGNLRQNFLSKRADE SADEWSVNDASPEKRFDSISTSALNGMGQNFFDKKYFDNFPEGAVSKRFDPISSSGTISGLQQNFLKKKD SDSRSAGGMGGVRLSFPRQTGSVDGMTSSKRQFDSISGGSGIGGLGQNFLNRRQFDSINEGDIGGMEQNF LSGKRFDTISSGKMAGFNQNFLGKRQFDSISTGEMSGMDQNFLGKRGFDSINSDSRISGMGQKYLVRRSS SLEPLPSAEVSEQPHDVYKRSPEDVTSLEASHDSAVVTGDTSEPIGPEHRFIKRSFDSISRFSGGMAGMS QNYLSRRASNTRALDSNSQPSSSSASYTSRGTNDVTSLAGVDNDHELWRILDKKNLDSISRLGAYFRLSS ELLRLAVCQTRGPGRARLQPTSPRHRSSLQSMGLPKIGVRRFLRD PleurinGene Bank Accession Number: 56200040

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Reference: Moroz, L. L., Edwards, J. R. et al. (2006). Neuronal transcriptome of Aplysia: neuronal compartments and circuitry. Cell 127(7), 1453-67. CDS: ATGCAGTCTCACGTGAGCAGCAGCCTACTGGCCCTGTGCGTCCTGACGGGCAGCGCATGCGCAATGTTCTACACCAAAGGCAGCGACAGCGACTACCCCCGGATCGGGCGTCGGAGCTTCTACACAACCGGAAACGGAAACCATTACCCGCGAATAGGACGACGGGATTCCCCTGGCGCAGCTGCTGGCACGGACATTCTTTACCCGAACACGGCTTCAGGGTTTTCCGATTTCTCGGCAGGCGCTCCGGCGGTGAAGAGAGGGATTTTCACGCAGTCGGCGTACGGTTCTTACCCGAGGGTAGGACGAAGGAGTCAGGCGGGAGATGATGGTTCCCAGGAAGGTCTTCAGACTGCGAGATCTAAGATTTTCCAGAAGATTACGGGGCTGGACGGGCCGGTGGAAGAAGAGCTCGGGGATGGATCGGCCGAGAGATTGGAAGCTGACAACCAGATTGGGCTTGAGCTCATGTTCCTCGCCTTTGACCTAGATGGTGATCAAGCTTTGTCAAAATCAGAGTTCCAGTCCGGGATGGAAAAGTTCCGACGACACAACCCACTTTGCTAA Protein: MQSHVSSSLLALCVLTGSACAMFYTKGSDSDYPRIGRRSFYTTGNGNHYPRIGRRDSPGAAAGTDILYPN TASGFSDFSAGAPAVKRGIFTQSAYGSYPRVGRRSQAGDDGSQEGLQTARSKIFQKITGLDGPVEEELGD GSAERLEADNQIGLELMFLAFDLDGDQALSKSEFQSGMEKFRRHNPLC Preprogo nadotropin-releasing hormone-like protein

110

Gene Bank Accession Number: 158906123 Reference: (Zhang et al., 2008) Prothoracicostatic peptide 2 Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: CAAATGTCCGTTTGGGGTAAAAGAGATGAAGATGGCACTTAGATAAGCGGTGGAAACAAATGTCTGTTTGGGGAAAGAGGGATGGAGATGGCAACTTAGATAAACGGTGGAAGCAAATGTCCGTTTGGGGGAAGAGAGACGAGGATGGCAACTTAGATAAACGGTGGAAACAAATGTCTGTTTGGGGAAAGAGAGACGATGACATAGGGGACTTAGATAAAAGATGGAAACAAATGTCGGTTTGGGGAAAACGCAATGGGGCTGATGTTGGTGGTGATAATTTGGACAAGAGGTGGAAACAAATGTCTGTGTGGGGCAAGAGGTTACAAGACCTGGATTCAAACAAAAAGTGGGCTCAAATGTCAAGCTGGGGGAAGCGGGGGTTTGTGGGAGATGTAAGCGACGTCGATAGCTTAGTTAACAAGAGATGGAAGCAGATAGCCGCATGGGGCAAACGACTGAGCCAGGACGACGATCTCCAGAAAAAGTGGAAACAAATGCCTGTTTGGGGGAAGAGAGGCGATACCAACGACGTCGACGATAAGAGATGGCGAGAAATGGCTGTTTGGGGGAAGAGAAACTCGGAAAATAACGACAACCGAAACATCGTAAACGAAGGGAGCGACGAAAACACTGCGGACAAGCGATGGAAACAGATGTCAGTATGGGGGAAAAGGGATGCCGGGACGGCTCCAGCCGCCGTCATGGAAGCACCATCTCCAGTGTCATCATCATCATCATCCTCATCATCATCACCAACATCTTCCTTATCGTCCCCCTCATTATCAGCCTCAGCCGCAGAAGCAGCAGCTGCTGCAGGGGCGCCGCGCGTGGACAAGAGGTGGCCCGATAGCTCCGTCTGGGGCAAGAGAACGTACAGGCACATCAGGAACTGGCGAGACATGGACATGTGGGGCAAGAGGCCCAGCTGGTCGAGGACAGGCTTCACCAGCTGGGGAAAACGCTCTGGGGCCGCTGTGGACCTGGCTCAGTTCAAAGACTATCTCCTAAACAGCTCCCAGCAACAGCAACTGCAACAGCAGCAGCAGCCGTTGATAGGTGAGTCGTCAACATAATCAATAAAAATGAAAAATAAAATGACGAGGATGAATAAG Putative pheromone-2+

Gene Bank Accession Number: n/a

Reference: n/a Partial Clone: TGGCAATGGTTTAAAAGGCAGTTAAATCGCATCTTTTGGTTGAGACATATTTATTAGTTTTCTGATTCATGTAATATCAGTAAAACTATATAGAGAGAGAGGATGGGAAAAGAAAGAGGGAGAGAGAGAGGGGGGGGGGAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAGTAGAGACAGAGAGACGGAAAGGGGGAGATATATGGAGAGAAAGGGGGATAGTGAAATTGTCACTATTGGAATTAGGTTCTTTATACTATTGTCAAACGCATTGTACGTTCTTTTGTGGAAATGCGTTAATTATATTAAAAGTAAGGTATCATTTCTAAATAGCGATTGTCAATCCTGAGCATGCATATATTATTTTACCTATCTCAACAGAGAAGTTCTCGACGACCGCCTTCCCCACCACCATCACCCCACGCCGCGTCAAACCAGTGACCACAGACCTCCCCAGCTCCCCACCGGAAGCGACCACCAACATCTGCGACTACCTGTGCTCCGTGCAGCTGGGTGGTGATGCCTGCCAGTGCAGTAACCCCAGTCTGCCCGGGAAGAAGTAGTCGGCGGTTCGAACCCCGCCCGAAACGATGCTCTCACCACAGAATCGTCGGCGCACCTAGCTAGACATCTAGAACGTACCGCTGTTTGAACGACTTGTCCCGACACAAGGGGCGCTGTCTGTTCCAAAGACGTAGAGCTGAGACTTCCGGTTGTTGTTTTATGTCGTGAATGTATCCAATACAAGACGGAGCATCTTTCGCAAACGTTTGACTTTTGTTTGAGGTCTCTGTTTTGAATCCAATGGCTTTTTAAAGACTATATACAAAGTCTTCACATAATTTATTCTTCCATACGTTTTGCTTTGTCGGTTGTTGATACTTTATACGGCCTGTCCGGGTGCGTTTTGGAAGTGGCTGTTTTTTGTTGTTGTTTTTTTTGGTGCTTATTTTCCCTCTTCATAAAAGAGAGTAAGGGCAACTATTTTGTTGCACTTATTGTTGTGTTTATTGTTTCATTTTGCTGGGGATGTTTTCCTCTTTTGGGAAATTGGGAACAACTGTGCTTTCGCACCTTCCTGCTTTTAATTTCATTTCTTTTCATTTCCTTCATTGATGTTGGTTTTAAATAAAAGCACATCCAATATCGCCCGGTTTGAGCTCATCTTTTGTCTTACAGATTTACCTAAGTTTTGAACTCTCTTTTTTTATAGGTCAATACTTGGTCAGTCTGTGCCAGCGTATATAAATCTTTGACAACCTATACCAATACGTATCACTATTAACCCGTCTACCCTAAAGCTACACAGTTTTATCTGACTTTTGTGACCGTTTTGAGTTACTAAGACCAAGAGGCAGAGACAAATGTTCTATCAGCTCTTTTGAAATCGTAGACAATTCGAGCCGTATCATGATATTCTAAGAACTGCACGTGAAAAAGCTTGACGTGTTGTGAACTCAAACTTTCAGCG

111

TCAAATTCTCAAATTAGGCCCTGAGCAGATAGAACTTGTGATTCTGGACAATAATCGTCCACTTATTTTCATAAGCTTGGGCTTTCCCCCTCGTTT R3-14 peptide 2 Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: ATTAGGAAGGGGGGCCGATCGCAGCGGCCATTTACCCCACTATAGGGGACCAAAAGGGGGGTCCCCGCCCCCCCCCCTAGGTTGGACGGATATGTTTAACTTTGAATTGAATTTCTTCGGGGCGGAGGCGGTCGGGGTTTAAATGCAAGAAACCCATAAGGCTTTCCAGGTTATTCCCCCTTCGGTTTTGGGATTTCCCTCCCCCCTGAGGATTATAATTTGGCAGCCCCAGTGAACTTAACTGGTCGGCGGGGGCTTAAAACATCCGCAATTAAAATGAATGGAAGGGCCCTAAAATTGCCCTCACCTAGTTTAAACAACCGTATGCCCTGCTCACCGTCGAACGCTCCGTATACCGGACTTTAGCATGGAGATTATCAGGGGAGAAAGGGGTGGTGTTTTGGGGTTTTCCTCTCTACAGCTTTTTTGACGCACCGAGCCCCCTCACCGATGGGGGGGCTCCAAGTTAGTTTGGCCGTTTTCCTCCGCCGCCAACCTTTGGATTCATTCCCGCTTGGGTCCTCTCGGTTCAACCAGAACCAAAGACTGGCTTTTTTTGCTGTTTCCCGAAGGGAGGTTGTGAGTTTGTTTCTTTTTTTTGCGGGAAATCCTGCCCCTTTCATCCCCTTTCGGGGGCTTGGTGGTACAAAACCTCCGCATCCCACCCCTAAATTAATTGTTTTTAGTAGTTTTATAGGGGTATACAATTCATTTAATTTTTCTGGGGACGTTTTTTTTCCCTTTGACAGTTTTGGCAGATTTTAAAAACAAACGGGAAGCAGAAGAACCCTCCGCCTTCATGACCCCCCTTTCCAGACAAATTGGGCAAAAGCACCTTTGGGGTGCCGTCAACCCCGACCGTAAACCCGGCCCTGGGTCCGGCCGTCACGGCCGCTTCCTCATTTGTAACCGAAACCGATACGGGGGGGGGCATTTTTCCGGGGC Schitosomin-likeGene Bank Accession Number: 56200042

+

Reference: Moroz, L. L., Edwards, J. R. et al. (2006). Neuronal transcriptome of Aplysia: neuronal compartments and circuitry. Cell 127(7), 1453-67. CDS: ATGATGAGACAACTGTTCGTCCTGGTCATCGTGGCCGGAGCCGTGCTCGCCGACAACTACTGGTGCCCCAAGGCCGGCGAGGCTTTCGAGTGCTTCGAGTCGTCCCCCACAGAAAGGTTTTGCCTGACCAACGGACGCGAGACCGCTGTCATCTGCTCCAAGTGCAGGAAGAAGTTCGACTTCTGCCGTAACGACTTCGTCAAATCCAAGAGAGCCCACACTGACTGTGGAGCCGGTTGGGAAAGCACTCCCTGCACCCACGACAACTCACACGTGCCCGCTGTCTTCCCCGGGAAACTTTAA Protein: MMRQLFVLVIVAGAVLADNYW CPKAGEAFECFESSPTERFCLTNGRETAVICSKCRKKFDFCRNDFVKSK RAHTDCGAGW ESTPCTHDNSHVPAVFPGKL Second (short fragments obtained from MS data)* Gene Bank Accession Number: n/a Reference: n/a Partial Clone: NTGGGATTCGCAGTCGGTACTTTTTTTTTTTTTAGCCTCTTCTTTTTAGCCATTCCTAAAGAGTGAGAGTTTTTTTATAATTTAATTTTTTATGAACTTCAAAAGGTGGAAGGACAAGATTGAATGATTTAGTGCAGAGGACACTCAAAAAAAAAAAAAAATTCATCTCTGCTAATGAGCGATTTCTGCGAGACTAAAATTTGTTTTATTGTGGGGTTGATTTGTAGCTCTACGTTCTGGGATTGTACGCCTCAAGAGTATAATCACCCCTGGCTTGAATTTAAGCAGATCTCACTACGACTACTGCCAGTGTGGGTGGTGAATTGTTTCTGTGTCCAGAAAATGTTTCCTTCACCACAAACATTTCTCAGTTCTGATCCGTTTATACTTCTTTTATGATTAATTCATTTCCTTTGTGCCGGTATCTCTCGTGATTCTCTAAAGAAAGAAAATTCACAAACTATTATTACAGTGTTCCTTTCACCTGATGTATCCCGACAAAGGTACCTGTGGTTAGGCTACCGGACCCGTGGTCGTGAATTGTGGGTCCCGGGGTTCGAGCCTCAACTCGGTCCGATGTTGCGTCCTTGGGGAAAGGCGTTTGAAGTTTCAAAAATGTC

112

CTATTTGGCTTAGGCCTTTCGGAGAAGGACGTTAAACTAGAAAGCTCCTGCTTCTATCGACACTGTTAGGTTATAATTCAAGAGGCAGGAG Secretin (a peptide hormone, primary effect is to regulate the pH of the duodenal contents via the control of gastric acid secretion and buffering with bicarbonate) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: GATCAGTCAGTTTTCTGTGGCATAGTCTCTCAATTTGGACAGCTCATTTGTTGTACCGTCTTAAGTAAAATAAAAACGAAAAAGTAA SFY1-like peptide* Gene Bank Accession Number: n/a Reference: n/a Partial Clone: GCGCTGACAGGATTGCTCTGCGCTTCCTGGCGGGAACAGTACAGTTCGCGTGGGGCGTGGTCAGTGGTACAGCACGTGCTCCCCCGTCAGTGAGCTGGGCCAGTGTGTGCGAGGGGAAGCGTCCTTGGCAAGTGAGATCACGGTCAGAGGTCGAGGCTTCCAACGGCGTGACACTAAGATCCTGTGTCAGTGCCCCATAGGGAACGACCTCGAACAGAACCCCGTGGAAGTACGCAGAGAGGAAGACTCGCCGCGCATGACAATCATTGTCTTTTGTGGTGAGGATTCTCAAGCGACAGGCGAGATGAAAAAGAGAGGACGTGGCATGTCCGCGTTTTACAAAAGAAACCATGGTCTCCAAAAGGGCTTCTTCTCTTCCTACAAACGTTACCCCAGCATCAGCGCGTTTTATAAGAGAAGCCCGGTATTGGGGACCGTGTTTGCGCGTCCCGACCGAAAGCTGCACTCTTTCTACAAACGAAGTGTGGCGTCTGACGTCACTTCTGAAGAAGAGGACCAAGACGTAACGTCAACTAGCCGCTTTGCGCGAGATCTGAGTTCTTTCTACAAGAGAGGATTAGGAGTTGCATCACTGAGCTCGTTCTATAAGAGAGATGACGAAACAGCGGAGGAGGAAGATGGGGATGAGCCAGACAAACGAGGGGGCATGAGCGCTTTTTATAAGCGAGGTTTAAACGGCGTCAGCTCGTTTTATAAGAGAGGATTATCTCGTCAGAGCTCGTTTTACAAGAGATTTCCTCAGTCTGTCAGCGCGTTCTACAAGCGAGAATTTGGTAACCCGGATAGCAGCTCTGCTCTATTAGATAATAGTTTGGATGGTCAGGAAAAGAGAGACTTTGGATCTGGGTTGAGTTCTTTTTACAAAAAAAGCGGCCCGTTCAGCTTTTACAAACGCGGCATGAGCTCACTAAGCTCGTTCTACAAAAAGCGTGGACTTGACTCTGGCTTGAGTGCTTTCTACAAAAGAGTTCCTGGCTCGGCTTTGTCTTCGTTTTACAAACGATCAGGCCTTAGCTCCTTCTACAAGCGACCTCACACGTCAGGTGGCATGTCCAGCTTTTACAAACGAGCGAGAGGGTTATCCAGCTTCTACAAGAGGGATGATGATTCTGAAGACACAGCCATAGATTCTAATCACGCCCCCATGATGTCCTCAGAGGATTCATATCACCGTAGC SFY3-like peptide* Gene Bank Accession Number: n/a Reference: n/a Partial Clone: LRGSYHRSKRSIKTDDHEATVQRRSTDALSSFYKRGLQGVSSFYKRASGQGVSSFYKRSPFGEMSNFYKRSPFNGVSNFYKRGAESYSSFYKRMLPGSQQSRFYKRTPNSLSSFYKRSPNNDVSSFYKRSPYDDVSSFYKRSPYDDVSSFYKRSPYDDVSSFYKRSRQSSFYKRFPQSVSAFYKREFGDPDSSSALLDNSLDGQEKRDFGSGLSSFYKKKRPVQLLQPRDELTKFVLQKSVDL Somatotropin I precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: TGGGATTTTATAGATTTTTCTCTGCTGAAACTTTTCTTTCTACCAGAGGGCAGCTCCTTTTCAGCCTTTGCTCACCAAAACTGCTGCTATGTGGCCTTCTTCTATGAGATAGGGCACGACTGATGTGTAGAACACGGC

http://en.wikipedia.org/wiki/Peptide_hormone�

http://en.wikipedia.org/wiki/PH�

http://en.wikipedia.org/wiki/Gastric_acid�

http://en.wikipedia.org/wiki/Bicarbonate�

113

CATTGTGGGTAACTTACCATAAATGGGAAGACTTCCCGCCCCTGGCTAATCTCTTGAACATGGCCGCCGATGCTGCTCTTTTTTTTTTCTTACAGCTAAATATTCTATTCATGTTAGATTTTTTCAGTCCTAAATATCAGGTTCACATAAAGTCATAAATTAACATTGGCCTCTTAGGTAAGTGTTGAAGTTATATAACGTGGGCATTAGAATTCGTGGGGATAGCCATCATCATCATTATAATCATCATAATTTTTCTGGTGGTCATATTAGGGTGGGGCTGTGTGCCTGCACGTGGTGGGGAGAGCAGTTGTCACATTCTATGGCCCTATTTATTGGCATCGTTCTCTGTGAACATTTGGTTGACTGGATTTGATTTATTTGAAAACCATGAAGCATCGCT Somatotropin precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: ATAATGTTTCATTGAGATTGCCGGTTATCCTTAGATTATCCGGATTTTGCCCACCTCTAGTGCTTTGCCGCGCACGCCCCCCCCAAAAAATGCAAGCTTGATTTCCATGTTGGGAGATTTTTTTTTTTACAGAGAAGCAGAGTCCTTATGCTGGGACGTTGTGTTTCTCTCAGCCCAGGTTGGTCGTTACAGGCAGGATGAAGCCCGCCTCCTAAGTGATCTAAACTGTGTCCATAGGACCACAAAAACAACTTCATTTCATTAAACAAAAAAAAAAAAAAGTACCGACTGCG Suppressor of lurcher protein 1 (Accessory protein required for glutamate-gated currents. May participate in the gating of non-NMDA (N-methyl-D-aspartate) ionotropic glutamate receptors such as glr-1) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Prediction: GGTAGTAGGCATGGAATCCCGTCGAGTCGTAGATGTCGTTAGATTTGAATATCACTCGGAAAAAGGACCCATCTGACCAGATCTCTCGTTTCTCCTTTGATGGAGTGCCACATAATCTGGTCATCTTGCGGTCTTCGGTCTCAGCAAAGTTTGAGAAGGACACGTAGTCACTGTTGGTGCTTTCCTCACACCTAGGATTATTCCATCCACATCAAAGTCAACAAACTGGATGTGCACCCGCTCCTTGCCCCGTCCGTAGAACAAATAATGGCACTCTGTGTTACGGGGA TACTGCCCGTTGTAGTTTGGTGATGTGATCTCACCGGTGGACTGAACCGTACTCTGGTAGTTGAACACGCACGTTCCCCTGTTGTCTTGTTGCCCCTCCAGTATTCCAAAGTTTGTAACGAAACTA Theromacin+


Reference: Kohn, A.B., Moroz, L.L. (2006). Unpublished. CDS: ATGGACAAGAAGGCA GCAAATGGAGGAAAAGAGAAGGGCCCCTTGGAAGCCTGCTGGGACGAGTGGAGCAGATGTACAGGTTGGAGCTCTGCTGGCACTGGAGTTCTTTGGAAATCTTGTGATGACCAGTGCAAAAAGCTGGGGAAAAGCGGTGGAGAATGTGTGCTCACTCCCTCTACTTGCCCATTTACACGTACCGACAAGGCTTACCAATGCCAGTGCAAGAAGTAA Protein: MDKKAANGGKEKGPLEACWDEW SRCTGWSSAGTGVLW KSCDDQCKKLGKSGGECVLTPSTCPFTRTDKAYQCQCKK Third (short fragments obtained from MS data) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned

114

Prediction: GGCTCGACAAGTAGACCTGCTTCAATCCCAGAGGTGAATACTGAGACGAATAAGCATGCAGCTCTTAATACTTCTTGCTGCCGTTGTGGGTGTATCCCTGGCCGCCCCAAGCCCTGATGTTTGCTCCAACGTCAACGCTACGGCTATTAAGTGTATGACAGACAACGGAATTCCTCTTCAAGATGTGCTCTCTATGCAGAAGGCTGCTTTGTCTGGGAACATTGCAGCAATGAACCTGACCCGCTTCTGTGGCGACAAAAAAGATGGCTACCTGAAAGGTGTGGAGTGTGTAATGAACTCCGTGGCTGTGTGTATCCCATCGTTCAAACAAACATTAGGAGACTTGGACTTCAAAAAAATGCTGGATGTGCAATGTACCGACAAAAACATCAAGTACAACTGCCTGGGCCTGATGCAACTGCCTGCTAATAAAGCTGTTGTCCAGACCTGTGTCCAGAAAGCTTTTGAGAATCTGAAGGATTTGTCTTCAGACAAGAGAGTTTGTGAGGCGACCAAGAGCGCCTACGTGTGCGAGGATGCCATTGTGAGGTCATGTGATGTGCACACCGCTGATGTGTACAAACAGTTCATTGACCCTTTCATGAAGAGGGACTGCGATAACTTTGTCCTCGGGTGAATGTGACAAAGTGTCCAGGACAACCGGGGTATCAAAGGATAAAAAAGAAACTCGCAAC Whitnin precursor (SPTR)Gene Bank Accession Number: 56200044

+

Reference: Moroz, L. L., Edwards, J. R. et al. (2006). Neuronal transcriptome of Aplysia: neuronal compartments and circuitry. Cell 127(7), 1453-67. CDS: ATGGAACTCCAAGCCTGTAACATTTTTGCCCTTTTCGTTGTGGTTGTGACGCTCTCAGTTGCCAGCAGTCTCCCTGCCAGCCGGACGGACGATGTGCTGCAGGAGGCCTCGGGTCTGGCTTTGAACAAACGGCCAAAGTACATGGACACCCGACGGGACCTTGACGTCTTTAAAGATCTGGTGCTCATCAGCATTCAAGAGCTTGTGGACGAAAACCGCCTGAACCCCGCCTTGTTGCCAGAGGAAGATGCCCCCAAGCCCGTTGAGAAGCGAATGCGCTACATGGGAATCTGCATGAAGAAACAGTACAACAACTTCATCCCCTTCCCCTGCCTCAGGAGTGGTCGCTAA Protein: MELQACNIFALFVVVVTLSVASSLPASRTDDVLQEASGLALNKRPKYMDTRRDLDVFKDLVLISIQELVD ENRLNPALLPEEDAPKPVEKRMRYMGICMKKQYNNFIPFPCLRSGR Signaling Molecules

Wnt-2 protein precursorGene Bank Accession Number: 46981372

+

Reference: Brown, B., Kohn, A.B et al. (2007). Unpublished. CDS: ATGAGATCATCACCAGGCTATCCACTCTTCATTTTGGAAGTGGTCCTACTCCTAGGGCTTGTTGCTAAGGAAGTTTCTGCATCTTGGTGGTTTATCAGCCAGCTAAACATCCATGCCCTGGGCCCTGGTGTGGTGTGTGACAACATCCCCGGTCTCGTGGGCAGACAGCGCAGGCTATGTCGTCATCACCCTGACGTCATGGTGCCGCTGAGCGAAGGCGTACGCCTCGGAGTGGAAGAGTGCCAGTTCCATTTCCGGAACCAGAGGTGGAACTGTAGCACGCTGGAGAGGGATACCTCCTTGTTTGGGAAGGTCATGCTAAGAGGCAGTCGCGAAGCTGCCTTTGTGTACAGCATCTCTTCCGCTGGCGTGGTCCACGCCATCACCCGTTCTTGCAGTCGTGGCCAGCTCATGCACTGCGCCTGCGACCCCACCAAAAGAGGATCTGGCAGAGACAAGGGAGGTGCCTTTGACTGGGGAGGATGTTCAGATAATGTTCGCTACGGCAGTACTTTCTCCAGGATGTTCATCGACGCTAAGGAACGCAAGCAGAGAGACGGGAGGGCTATGATGAACTTACACAACAACAGAGCCGGCCGAAGGGCGGTGAAGAAGTTCCGCAAACTGGAGTGCAAGTGTCACGGTGTGAGTGGATCCTGTACCATCCGCACGTGCTGGCTGGCCATGCAGGAGTTCCGTCTGGTGGGGCAGCACCTCAAAACACGCTACAACGGGGCCACGCAGGTCATGATGAAGCAAAACGGCGCAAGTCTCATTGTGGCAGATAAGAACCACAAGCGTCCTACGCGCTCTGACCTCGTCTACTTAGAATCTTCACCCGACTACTGCGTTGAGAATTCGGAAGTTGGATCGCTCGGGACTACAGGGCGCACGTGCAACAAATCTTCTATGGGCACCGACGGTTGTGACATCATGTGTTGTGGGCGGGGCTACCACACACGCGTGGTGAAGCAACATTACAAGTGTGAGTGCAAGTTCCACTGGTGCTGCTTTGTACACTGCAAGGATTGTCAAAGGTGGGTGGAGCTACACACCTGCAAAGGCCCCGCCCCTCAGGGTCTCATTGGCTCCTGA

115

Protein: MRSSPGYPLFILEVVLLLGLVAKEVSASWWFISQLNIHALGPGVVCDNIPGLVGRQRRLCRHHPDVMVPL SEGVRLGVEECQFHFRNQRWNCSTLERDTSLFGKVMLRGSREAAFVYSISSAGVVHAITRSCSRGQLMHC ACDPTKRGSGRDKGGAFDW GGCSDNVRYGSTFSRMFIDAKERKQRDGRAMMNLHNNRAGRRAVKKFRKLECKCHGVSGSCTIRTCWLAMQEFRLVGQHLKTRYNGATQVMMKQNGASLIVADKNHKRPTRSDLVYLESSPDYCVENSEVGSLGTTGRTCNKSSMGTDGCDIMCCGRGYHTRVVKQHYKCECKFHWCCFVHCKDCQRWVELHTCKGPAPQGLIGS Frizzled-related protein 2Gene Bank Accession Number: 46981374

+

Reference: Brown, B., Kohn, A.B. et al. (2007). Unpublished. CDS: ATGAGCGGGCGAATACAGCAGCCCAAATGCGTGGACATCCCCAGTAATCTAACTCTTTGTCAAGGAATCGGATACGAGACGATGAGGCTTCCCAACCTGCTCGATCACGATTCTCTGAAAGAAGTGACACAACAAGCAGGTTCCTGGGTGCCTTTGACAAGGATCGCCTGCCACCCAGACACAAAGGTCTTCCTCTGTTCCCTGTTCTCTCCAGTCTGCTTAGACAGCCTAGATAGGTTGATCTATCCGTGTAGGTCGCTGTGCCAGAACGTGAGGGCCTCGTGCGAGAGTCGGATGACTATACATGGCTTTGAGTGGCCAGCCATGCTGGAGTGTGAGAAGTTCCCACTCGACAACGACATGTGTATAATGCCCATACACGACATCAAACCTGACAACAACTGCACCGCTTGCAAGCACCCACAGACACACGAGGCTCTTATTGACCACTTTTGCCGGTCAGATGTTGCTATGCGGGTCACTTTGAAGGATCGCCGCGTTCTGGGCACCGACTTGGAGCTTGTCCTTCGAAAACGTCGCAAACTGTACAAGTTCGAGAACATGGAGAAGAAAGAGGCGAAGAACCTGCCAGTCGTCATCGCTGGAGGTACTGCGTGCACATGTGAGACCATCAACGACACTCAAGGGAAGTACCTGCTCATGGGCAACAAACGCAACGGGAAAATCGTCATCAACTTCGGCATGGCTTGGAAGCGCAAGGATAAGGAGTTCCGTAAAGGTCTCCGCGCCATCAGAAAGGGCGACTGCTAA Protein: MSGRIQQPKCVDIPSNLTLCQGIGYETMRLPNLLDHDSLKEVTQQAGSW VPLTRIACHPDTKVFLCSLFS PVCLDSLDRLIYPCRSLCQNVRASCESRMTIHGFEWPAMLECEKFPLDNDMCIMPIHDIKPDNNCTACKH PQTHEALIDHFCRSDVAMRVTLKDRRVLGTDLELVLRKRRKLYKFENMEKKEAKNLPVVIAGGTACTCET INDTQGKYLLMGNKRNGKIVINFGMAWKRKDKEFRKGLRAIRKGDC Hedgehog Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: TGCTGTGAGGCAGTGAAATTGGGGACTGGTGCTGTCAGAGATGCTGGAGGGTGTCTGAAATATGATGAGGTCTACATGTTTGGCCACAAAGACGAGGTTGCCGTCTCCGAGTTTGTGATGCTGTTGACAGAGACAAAGTCTGTCTGTGTGAGTAGTGAACACAGCGTGTACTGCTCACGGGCTGGCCAGGAAATGTGTGTTGCTGCTGGTAATGTAAGAGTTGGGGATGAGCTTCTTGTACTGGACGCTTCGTCTGCACGTCTGGTGGCCGAGCCTGTGGTGAGTATTGGGTTTGAGAAGAAGCAAGGATTGTTTGCTCCGTTTACCCTGAGCGGCTCTATCGTGGTGGACGGGACTCTGATGTCATGCTACGTGAACTCCCCGGCCCCTGCCACCGCCCATGCTCTGCTGTGGCCAGTGCGACGACTGTACAAGTTGTCTCCCTGGATGCTGGAGTTTATCAGCGGCTCTAGCAAACAGGACACTATTCCTTGGTGGGCTAGGACCGCTCTCAGGTTTCTGTAGAAGAGAACTGTTTGACCCCCATGAACCTGGTCGTGCACATTTGAATCAGTATTGGAAGTCTTTCAGAATCTTGTATGTGACCTGTAATTGCATAGTTTCTGACATGTTGCAGATCTGTATCTGTGTGTTGAAGCCCAAAATGCATGACTAGGGTCAACCCTGAACTAGGCCCTACCTGACCTGTCTTCCTTGAATTATCCTATCCTTACCATATTAAGTG Chordin-like protein Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned

116

Predicted: GAGAGAAATACAATCCTTCTCAANTCGCAGGAGACGGGAGAAACAGATTTTGTTGTGTGATAACGTCAGATTATCTCCTCGAGGGAAACGCGCGCTGAGAAATAGTAAATAGTGAGCCGAGGAATTTATCTATCGTTTTGTGCTTCTTCGCGCAGTCTTGTAATTGAAGAGTGGGCCTCCTTCCAACTTTTCTTCCCTTCTTCTGTTTGACATCACTGACCGCAAGTCGAGTGTTCTCTGGTGTTGTGTGTTTCTTATAATTGTGGAACTACTGCAGAGGATTTGTTGTATGGACACTTCGGATTTTTCAGACGTTTGGAAATAATGCGCGAGTGTTTTGACTGATTTCTGTACAGTACCTGGAAAATAAACTACGCACGTTCTGTGATTCTTACGGTACACTTGAAATTACTGACAGACAGTTTTCCATTTCCTGACGCGACCCCGTTATAGGATGGGGCAACCGCCTACTCAAGCGGGCTGCTCTAGCAACTCGTGTGTCTATGAATGCGTCCGTGAGTCTGTTCGTGAGTCTGTACATCGTGGGTCAAGTCACATAAAGGTGGTCAACAGCTGAGGAGGTCTAGAGAGGACGAAACCAGAGACAGCTGCCAGCCGCCCATAACATGCAGAAGATGACTCAGATGCTGGTGTATAAAGTGCTCATCTTCGTCATGGTATATCGCTGCAGTGGAAAGCCTTCGGGAGGCCCGTGTGTTTTAGGCGGAGAGCAGTACAACGTTGGAGAGACGTGGCATCCTAAAGAGTTTTCTTCCACCGCCAACTCCTGTGTCCACTGCACGTGCCTAGAGGGCGGTCAGATAAATTGTACCGGCGTGGACTGTCCTTCTCCAGAGTGCGAGGTGCCCAGATTTATCCACGGACAGTGTTGTCCTACATGTGATGTCCGGGGGGACGAGGCCGGAAGTCCCGAGACAGACATGCCCGGATGTGATTTCCATGGCGACCACTACGAGGACGGTGACATATTTCCGTCCAACAAGACGGCTTACAAACCCAAGCATGACAACCAGTGTGTGTTGTGCGGGTGTTATAGAGGTGAGGTCATCTGTCACCTGAAGACTTGTCTGCCCAGCCCCAGGTGTCGGCGGGTAGTCCGGGTGGATGACGACTGCTGCCTACAGTGTGAAGAGGAGTCCAGCATAGACGAATATTTCATGTCTATCGGGTCAGACAACATAAACCATACACTGGACGACGAAGACTGTCTGTCAGCGACAGGACGCCGTAAGAACGGCAGTACGTGGAAGCCAGTGGTGGGCGAGTACGGAGAGATGCATTGTATCGTCTGCTCCTGTTTAGAAGGTCAGGTCGACTGCAAGCGTCTGACCTGCCCTGACGTCACAACCTTGACCTGCGGACACCCAAGGCCACGACAGGACGGGTGCTGTAAGGAATGCCCTGAGAGCGACAGACAGAGGGACAACAG Wnt-16 precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: TTTTTTTCCCCCGTCTACTTAACATCATTTATGCAGCTGGTGACAGTAACGAAAGCACGACATTCCGCTTTTCGAAAGCATGGGCGTGTACCTACACAAAGCCACGATGGGTAGAAATAGCGCACTGAATGTCCATGCTGAAGTTCTCAGATTCTTGAACAGGACTCAGGAGTCCACAATCCTCTGGGATTTGATCACGCCTGTCAGAGTCCCAAATACTGCCAGAAGTGATTACACCGGTCAAATGACATGTGGTATTAATTGTTTTTTTAAAAGTAAACATGAAGGGACTTTCGTATCTAAAGTGAAGTACATTTTGGAAAAGTCCAACTTCATAGGAAAAGTTCCCAAAAATCATGACAGAGATTCCCAAAACAAAGGTGGAACCAGAATTTCTAAAATGAAGAAAATAATTGTCTCTGAGGTGTACTCATGTGCAGTTTTCAGACTGGACCAAGAAGGCTGCACTCCGAAAGAGAAGGCTTCACCGAGTATTTAAATGGGAATAAGCCACACTTTGGTGATCAGCTCGTCGAAAGAGCCAAATTCCTCTTTGTCGGTGACTTTTAATTAAAATTTTGCATCTACATTTAATAGTGGGGTATTATTGGTATAGATCTAGTATAAGCAGGGTGGTCCCATGCCCCTTTTTGGGTGACCACTGAATGCCCTTAATAATTTTCTTTTCAAAATCAGTACGTTGTCAAAAGTCCAGCATTTCTAGCAGAAAAAGGTCAGATTTTTGTGACACATTCAACAAGGGCTGGTCATGTCATGCTGAGAATATTTCTACACTAAAACATGCAACGCCTGACAAATGAAATACAACACCACTAGTCTTTAATTTTTCAAACATAAAGACTTTGTAGAAGCAGCTCCGTCGGTACAAACTGATTCACGTCAAGTGTTTAAGTATGCTCTTGCTAGGATTCATCATTTCCTATCACATCGTATTGCAAACTCCTGCTTTCGATTATACAATAAAAGCACAGGATGTTCACAAGGGCCACTGAAAAGTACTTCAGCCAAGAGAATTGGCGTTGATGGTAGGCTAATATATTGTTCTCACAGAGCAATACTGGCATCGCTCGAAAAATGGTTTCCTTCAGTGTAAAATAGTGTTACCAAGCTTATCCTCGGATACATTGTTTGAGAGGTTGTGAGGGAACGTTTCAATTTCGGCTTTAGAATGAGTTACAACTCCGTGGTCAGCAGTTTTCAAAGCTGTTGTTCGGGGACTTCATGCACCACCAATATCTTCCATAAATTCAATTGGACCTGCATACATTTAAGATCACGCACAGCTCTCCTGAGTTAAATGATCGACCATCGGTGATCCATGCCAGACTCAGTGAAAGGTTTCCTCAAGAAGAGACAGACCGCATGCGCTTTAAAACTTTTGTCGGAAACTCGGCGGCGCAAGGTGCTCCATTATGTAAATGACACTTCACCACATGTCCTGTCACGGTATACTTCTAATCACAAGTGCACACTCTTAAAGTCAGGAAGGAATTCAGTCGCAGTGACAGAATTCCTTCTTCTCGGACAACGAGTTTCAATGTGCACGCCACGCCATTTCTTGTTTCTCTTGGATTATAATATATACACAAAATCCAACACTTTTCGATTGACAGTGACGCAGGTGCTGTCACTTGCAGGTATGAATGTCAACCAAAGTTTCACAAGTCTTGCACTCAACGTAACAACACCA

117

ATGGAACTTGCACCGGCATCTTTCTACGTGTCTGACGACCTGCGTGTTATAGCCACGCCCACAACACAGCAAGTCGCAGCTCTCTGCCCCGTTCGCCGTCCGGTTACACCGGCGTCCCGTCGTACCCAGAATCCCTCTCTTCGGGTTGTTCCGGCAGTAGTTCGCTCGAGCC Wnt inhibitory factor 1 precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: TCATCACAGGAGTCCTCTGCTGCAGATTGCCCGTGAAGA GACCAAACATCGTAGTCAAGCCCCCAGGGCGTCTTCAGGTGTACAGAGACTTCATCTTCCTGCTGACCTGCCAGGCTTCCGGTATGCTCCCACCGCGTCTGCTCTGGTACAAAGATGGCCGCCGTCTCGCCAGTGGGAATTCCCGCATCTCTGTGCTCTCCTCGGGAGACCTGCTAGTGACGCTGGCCGTCGCTCGGACACCGGTCTGTACACCTGTGAAGCCATTAACGAGGAGGGCATTGATACAGCCAGCTCCTACGTCAGCGTAGCAGAGTACACGTCTGGGTGTGCAGACGACAGCACTGACGGTCTCCACATGCACAGTGACATCCAGGCGTGTGCGGGTGAGTGGAAGTGGTCACGTGAAGTCCGCCAAATCACTATGTGCTCGCGGCTGGAGGGTGTGTAATCCTCGTGACCAACGGAGCCTCAAAGAGATCACGTCCTTCGAGATGTTCGACCTGTCTGGCTGCTACGCTTACAATGCCGCCAGTCGGAAGAACAAGTGTAAGAGGTGCAAAAATAGCAAAATGTCCGGCATCGGGCGGGACTGCGGTCACGTGAGCTACTCCCACACTTCCTGTCTGGCTCATGGTCGCATCGACGTCTTTGCGACCAACCAGACATCGTCGTGCCAGTACACGCCAGGGCTGACCTCAGGCGTTCTCTGCTGCAAGAAACCCAAGAAGAGAAAAGGGGAGAAGTGTGTACCGCGGTGTGAGCACAAGGGCGTGTGCATCTCACACAACCGCTGTCGATGTGCATCAGGTTACAAAGGCGCCCGCTGCCAACTGCCGGTGTGCTCACCCGGATGTGGCTCCAAGGGTCAGTGCATCCGGCCCAACAAGTGTCGCTGTAGTGCCGGCTACACCGGCCGCACGTGCAGGCGCAAGACCAAGCCCTGCAAGAGCACCTGTCTCAACGGGGGGCGCTGCCGCAGGGGCAAGTGTAAATGTCCAGGGAGCTTCTGGGGGAAGGCTTGCCAGTACCCACTCCAGCACGTTTTGCTGACGCGACTCAACAGAACAGAGTAAAGATCACAATCAACTCCCGCTGATCAGCATCACCGGAGCTCTTGCGTTCTCTGCTATTCGCACAACACTGCCAATAGATCGAGCGGTCCGGCAATCTGCACTTCTTGTCTCGAAAAGATAGTGCAATGTTTAGTGGAACATTTTTTTACAGCTGACTTCGTCAATGCAAGTTCATGACTGTGTCCGTTCGGTGTAAGTCCTAATCCAGGAGGCAGAGGATTGGGCTCTTGTGTTCGCTGATTTACA GTAATATTTTGTTATGCTGTACCATCCTTGTTTGTTTACGATGTTGTAAAAGGAAAGGGAATGGCATAATATTCAGTACGCATTATCGTGAGTGATATGTTTCCTTTGTTCATGAACATGACATTAATGTAGGTGTGTCTGCAGTGGGTGCCCTTATTGTGGATTGAGAGACGA Secreted frizzled-related protein 3 Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: TATGATGGGAGTTGTGTTGTGTCGTTATCGTCTTTCCCATTGTCGTAATTGTCATTAGAGTCGCCTAGCGTGTCTCGACGGATGGAGGCAAAAATGGCATCATCATCATCATCGTCGTCCTCCTCATCATCGTGTGAGAGATCTGCTGGAGTGAGTCTTCTATCTCATATGGCGGGCGACATGCCAACGACGTCCTCCCATCGCCCTCGTGGTTCTGTATACTCCCCTCGTGCGTCGTTATCTTTCCTTTCTGTCCTCATTTTCCTGTATGTCTTCGCCGTTTGTCTAACTACCGCGGCCTCACAACAGTACAACAATGGTGAGGATAGCTTGTACAATTATTACTACCACCATGACAGCAACAACTACAACGGCATGGATGAATACGTGTCGGACTGGAGCCAGCATGAGTGGGCCAAAATGAGCGGGCGAATACAGCAGCCCAAATGCGTGGACATCCCCAGTAATCTAACTCTTTGTCAAGGAATCGGATACGAGACGATGAGGCTTCCCAACCTGCTCGATCACGATTCTCTGAAAGAAGTGACACAACAAGCAGGTTCCTGGGTGCCTTTGACAAGGATCGCCTGCCACCCAGACACAAAGGTCTTCCTCTGTTCCCTGTTCTCTCCAGTCTGCTTAGACAGCCTAGATAGGTTGATCTATCCGTGTAGGTCGCTGTGCCAGAACGTGAGGGCCTCGTGCGAGAGTCGGATGA CTATACATGGCTTTGAGTGGCCAGCCATGCTGGAGTGTGAGAAGTTCCCACTCGACAACGACATGTGTATAATGCCCATACACGACATCAAACCTGACAACAACTGCACCGCTTGCAAGCACCCACAGACACACGAGGCTCTTATTGACCACTTTTGCCGGTCAGATGTTGCTATGCGGGTCACTTTGAAGGATCGCCGCGTTCTGGGCACCGACTTGGAGCTTGTCCTTCGAAAACGTCGCAAACTGTACAAGTTCGAGAACATGGAGAAGAAAGAGGCGAAGAACCTGCCAGTCGTCATCGCTGGAGGTACTGCGTGCACATGTGAGACCATCAACGACACTCAAGGGAAGTACCTGCTCATGGGCAACAAACGCAACGGGAAAATCGTCATCAACTTCGGCATGGCTTGGAAGCGCAAGGATAAGGAGTTCCGTAAAGG

118

TCTCCGCGCCATCAGAAAGGGCGACTGCAAGCAGTTGTTCAAAACCGTCATCGACAACTTACATGAAGATCAGAAGCAACCGGGGTCCT Secreted frizzled-related protein 5 Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: TATGATGGGAGTTGTGTTGTGTCGTTATCGTCTTTCCCATTGTCGTAATTGTCATTAGAGTCGCCTAGCGTGTCTCGACGGATGGAGGCAAAAATGGCATCATCATCATCATCGTCGTCCTCCTCATCATCGTGTGAGAGATCTGCTGGAGTGAGTCTTCTATCTCATATGGCGGGCGACATGCCAACGACGTCCTCCCATCGCCCTCGTGGTTCTGTATACTCCCCTCGTGCGTCGTTATCTTTCCTTTCTGTCCTCATTTTCCTGTATGTCTTCGCCGTTTGTCTAACTACCGCGGCCTCACAACAGTACAACAATGGTGAGGATAGCTTGTACAATTATTACTACCACCATGACAGCAACAACTACAACGGCATGGATGAATACGTGTCGGACTGGAGCCAGCATGAGTGGGCCAAAATGAGCGGGCGAATACAGCAGCCCAAATGCGTGGACATCCCCAGTAATCTAACTCTTTGTCAAGGAATCGGATACGAGACGATGAGGCTTCCCAACCTGCTCGATCACGATTCTCTGAAAGAAGTGACACAACAAGCAGGTTCCTGGGTGCCTTTGACAAGGATCGCCTGCCACCCAGACACAAAGGTCTTCCTCTGTTCCCTGTTCTCTCCAGTCTGCTTAGACAGCCTAGATAGGTTGATCTATCCGTGTAGGTCGCTGTGCCAGAACGTGAGGGCCTCGTGCGAGAGTCGGATGACTATACATGGCTTTGAGTGGCCAGCCATGCTGGAGTGTGAGAAGTTCCCACTCGACAACGACATGTGTATAATGCCCATACACGACATCAAACCTGACAACAACTGCACCGCTTGCAAGCACCCACAGACACACGAGGCTCTTATTGACCACTTTTGCCGGTCAGATGTTGCTATGCGGGTCACTTTGAAGGATCGCCGCGTTCTGGGCACCGACTTGGAGCTTGTCCTTCGAAAACGTCGCAAACTGTACAAGTTCGAGAACATGGAGAAGAAAGAGGCGAAGAACCTGCCAGTCGTCATCGCTGGAGGTACTGCGTGCACATGTGAGACCATCAACGACACTCAAGGGAAGTACCTGCTCATGGGCAACAAACGCAACGGGAAAATCGTCATCAACTTCGGCATGGCTTGGAAGCGCAAGGATAAGGAGTTCCGTAAAGGTCTCCGCGCCATCAGAAAGGGCGACTGCAAGCAGTTGTTCAAAACCGTCATCGACAACTTACATGAAGATCAGAAGCAACCGGGGTCCT Interleukin-16 precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: AGCAGCAGACACAACTTACAGCAACACCAGCAGCTGCTCCTTCAGATCTCCNCAGCTGGAGTACAGAAGGTGATCATGCTCAAAGGGGCTACAGGAGTGGGCTTCTGTCTGGAAGGAGGCCTTGGCTCTCCCAAGGGCAATCTTCCCATTGCCATCAAGAGGATATTTAAAGGAGGTCCTGCAGAAAAATGTGGACAGCTGAAGGTGAAGGATGAGATCTTGGAGGTCAATGGAGTAGACTTTACAAACATGAGACACTATGAAGCATGGAACCACCTAAAATTCCTTGATGATGGAGAAGTTCACATCACTGTGCGACGTCATTAGTATATTGATCAAGCATCTGTAGAGATTGTATTTGTTAACAGCTGAATTGTCACACTCTGTACATCCTGTTTATACTAACCATATGTTTTGTGTGAGTATTTATTTAAAGAGCTTTTTGTATGTAAACCATATAAACAGGGACTGTTATGATTTGTGTATTTCTTGAATCTGTATGATAATGTCTGTTCTCTTGTATTCTGTGATGTTGAAGAAGCCTACTGTGGGCTGGTGATACCTGAGAAACATATGTAAAGATCACATGGTTTTTAGTGTATACTTGAAATATTATCTCTGACTATAGGGCTATTTATGGCTGACAGACAGAGCC Thrombospondin (promotes the development of new synapses) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: GACCCCGCGAGAGAAATACAATCCTCTCAATCGCAGAGACGGGAGAAACAGATTCTGTTGGTGATAACGTCAGATTATCTCCTCGAGGGAAACGCGCGCTGAGAAATAGTAAATAGTGAGCCGAGGAATTTATCTATCGTTTTGTCCTTCTTCGCGCAGTCTTGTAATTGAAGAGTGGCCACCCCCTCCCACCTCTCCTCCCCAC

http://en.wikipedia.org/wiki/Synapse�

119

CTCTGTTTGACATCACTGACCGCAAGTCGAGTGTTCTCTGGTGTTGTGTGTTTCTTATAATTGTGGAACTACTGCAGAGGATTTGTTGTATGGACACTTCGGATTTTTCAGACGTTTGGAAATAATGCGCGAGTGTTTTGACTGATTTCTGTACAGTACCTGGAAAATAAACTACGCACGTTCTGTGATTCTTACGGTACACTTGAAATTACTGA CAGACAGAAGATGACTCAGATGCTGGTGTATAAAGTGCTCATCTTCGTCATGGTATATCGCTGCAGTGGAAAGCCTTCGGGAGGCCCGTGTGTTTTAGGCGGAGAGCAGTACAACGTTGGAGAGACGTGGCATCCTAAAGAGTTTTCTTCCACCGCCAACTCCTGTGTCCACTGCACGTGCCTAGAGGGCGGTCAGATAAATTGTACCGGCGTGGACTGTCCTTCTCCAGAGTGCGAGGTGCCCAGATTTATCCACGGACAGTGTTGTCCTACATGTGATGTCCGGGGGGACGAGGCCGGAAGTCCCGAGACAGGACATGGCCCGGATGTGATTTCCAT Similar to Growth-arrest-specific protein (promote neuronal survival) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: TGAGGAA GATCATAGGAAAAGACAGTGGTGTGGGGTGAGGTGCCTGTGGGAAAGTTCACG AAATGAGTTGTTGTAGTGGTGCCCTTTGGTGATAGCTGAGAGCGGAAGAGTGGAGAAGAC ATCCTACCTCGTAGTGCTGTGAAACTCATGTGTCAGGATAACATGCCTCTGAGGTCTAAG ATGCGACCCCACTTCCTGCCTGCCCAAGCCCCACAAGTCACTGCGAGCCTGTCTTGATTC GTCACATCTGGCAGTAACATATGCTTTGTTGAAGGAAATGGCCGTACCGACGAGTAGGCC TTACATAACCTGGCATCTGTTATGTCCAGTCTAACTGCTAGAATGTCGACCAGTTGGCGA TTTCCCCCGATCGAACAGTACATGATTGTTTTAGTCATGTCCACGTCCATTTCTCTTTAC GCAGACTTAACCGCAGTCTCAGACGAGAGCTGCCACGTAGCTCAACTTAACTGCCACGCG AGAAGCGGCTGCCAGAGGGCGCTCAACAACTTCTTCATTCACTGCCGATCTGTCATCAAA GGTGACTTCCGGGACGTCTGCCCTACGGACTGCAAAAATGCCCTTGTGTCTCTCCTGTCC ACGGAGGATGAAGCAGGACTGGCGTTCATCAACTGCGACTGCCATCAAGCAGGTCTTTGC TCAGAGCGGAAAGAGAGAGTTGAGGTCTGCAAGAGGGAAGTGTTGGCGTCCATGCACGTG CTTGAAGACAACGCTCCCCCCGTGTCCTGCAATCTGGCCAGATGGATCTGCGAAGCTGAC ACGTCGTGCCAAACGGCTCTGCGTTACTACTACGACTACTGCTCCAAACTTTTCAAAGGG GTCAAGTGTACGTCGCGGTGTAAAAACAGTTTGTTCATTCTGTCTCGGCAACCTCACGCC AGCAAACTGAGGTCTTGCCTCTGTGACGGAACAGAAGACTACGACTGTCCCACGCTGAAG GTCAACACGGAGAATATGTGTTTCACTCCTAGGGCGCGTCGTCGCCATCGCAAAA GTGAC ATTGCCAGAAAAGGTGGGGCGAAAGTGCCGCAAGAAGGGCGCCCGAATGTGTCATCAAAA GATAAGCGCCGTGCTGGGCATGTAAAAAGAAGAACTCGTCGAAGAAAAGAGACGAAACCG GCCGCACAGACCACAGGGCCGACGCCAGCGCGCCAGGAGGAGG Similar to Growth-arrest-specific protein 8 (promote neuronal survival) Gene Bank Accession Numbe r: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: GTTACTGTTAGTGTTATACAACGCAGTACGTGACTCTGTGACCGTGAACCTGAGTGAGGT GAACGTGACCGTCCTATGAGTTGTGCCTTTGAATGGTTTTCGCAGTGGAATAGTGAAAGT TATTACTGGAGTGTTCCAGTTCCCAATACAGGATGAACTAAATTGATGTTTAGTTAGGTC AGAGACTATCCTCTTCGACATCCGGGTTCTGTTGCTCTGACAACCACCGTAAGCGTCCAT AACTGTGCTGTGGGACGACAACAAAGTTGCCGTAGTTTATCAAAATGCCTCCAAAAAAGA AGAGTGGCAAGAAAGGGAAGGGGAAGAAGGGGAAGAAGTCAGGAAAAACCCCCACTGTGA TAGATGGCATTCCCACAGAAGAGATGTCAAAGGAGCAGTTGGAGGAGCTCATTCAGAGGC TACGTGAAGAGCTTGAGAGAGAAAGAGAAGAGAGAAATTACTTTCAGCTGGAACGTGACA AGGTTAACACATTTTGGGAAATTACAAAACGGCAGTTGGAGGAGAAGAAAGCAGAGCTCA GGAACAAAGATAGAGAGATGGAGGATGCTGAAGAACGCCATCAAATAGAGATCAAGGTGT ACAAGCAGAAGGTCAAGCACTTGTTGTACGAGCATCAGAACAATATCTCGGAACTGAAGG CCGAAGGCTCCGTGGCTCTGAAGCAGGCTCAGGATGGTCACAATTCGTCTGAGATGGAGC

120

TCCGCAAAGACAAGAGAGCACTGAAGGTGGAGCTCAAGGAGCAGGAGCTAGCTCATGAAG ATGTCATCAAGAACCTGAAAAAGAGAAATGATGAAAACATGACCTCCCTTCGCAACAGCT TTGAGCGCCAAGCCAAAGAAATCGAGTCAAAATATGAGAAGAAGATGAGGGCTCTTCGTG ATGAGCTAGACTTGAGAAGAAAGACAGAAATTCATGAGATTGAGGAGAGGAAGAACGGCC AGATCAACACCCTTATGAAGAATCACGAGAAGGCGTTCAGCGATATTAAGAATTATTACA ATGACATTACATTGAACAACCCTGCCCTTATCAACACATTGAAGGGACAAAGTTGAAAAA AAAAAAAAAAGGACCGGCGGGCCCGGGGTGGACCCCCGGCTTAAACCCCAGGGGCGGCCG CCGCCGGGCGACCATATGGAAAACTCCCAACGGCGTGGAGGCAAACTTGGGTATTCCAAA GGGGCCCCAAAAACCTGGGGGAACCGGTCAAGGCGTCTTTTTGGCGGGGAAAANTTTNTT TTAGAAAACCCCCCCCAACCCCCCGGGGCCAGGGGGGGGGGGGAAAAGGGAGCCCCCGCC CAAATTGCGCCAAAAAGGGGGAAGAATAAAATATTGGGGGGAAAAACTTTTAAAAAAAAA AAAAAAAAAAAAAAA

Secretory s ignaling peptides/proteins found by homology search to Lottia genomic screening

Strong Annotation

Lottia Protein Model: jg i_Lotgi1_119019_e_gw1.30.87.1 NR Annotation: PREDICTED: similar to seleno protein N, 1 isoform 2 precursor [Canis familiaris] NR E value: 2.00E-85 Ac Annotation: Seleno protein N precursor_[0.0]_[6]_APL_all_052305.8412.C1 Ac E Value: 7.00E-69 Sequence: MVLFTLLCCLFIIGRHDPTQIPEEIVINIGDDGVLLFQQHDRDNDGYLSIQEFEPLVYRLLEINVSGPVYDVPISTDDEMITLKSYFIPIVKESMSKDLNDSVSIGLLRTMNSLHGLEKW QNVNLQWMNFGASHFSGFLPKDVDSIMLGSSYFIINVEKGLFNAALSSNRYYPPKVTTNESIIVHRLLTLFHPRPFVVSRFPAQSSVACVRAYNDKYLDIVFRIHAEFQLNEPPYHPFWFTPAQFTGNLIISKDGKHIQYFNLYVPNNKRLNIDMEWLNGPNESENMEVDIGFMPLMQLNSTQSSVPVKNLEEYELLEPLPERPQHKTEGENIEWMATIDLEDAKSSLEKALYPFKKVPYYNFTEAFKKAEDNKKLVHSILLW GALDDQSC* Lottia Protein Model: jg i_Lotgi1_121665_e_gw1.37.7.1 NR Annotation: hedgehog [Patella vulgata] NR E value: 1.00E-171 Ac Annotation: Sonichedgehog protein precursor (SHH) (HHG-1) [Contains: Sonic hedgehog protei_[3.65288E-39]_[1]_L7ALL-ET3V2MC01DELVF_253 Ac E Value: 3.00E-32 Sequence: MKLFFSFQSVCSWTFIFLFITSLTHACGPGRGSGSRRRPRKRTPLVFKQHVPNVSENTLGASGISDGKIRRNSEKFKNLVKNENPDIVFKNEEGDGSDYLMSRRCQDKLNSLAVSVMNNWKDVRLRVTEAWDDSPNSHAKDSLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDW VYYESRGHIHCSVKSDSSVAIKIGGCFPPTGSVQTLHGWKTMGQLTVGDKVLSINSAGQLEYSPVIAFIDRNDLEFEKYLTLHTEDDTDITLTSKHLIYASGTNSSNFESYDVVYADDIMEGDHVLITSSEKGAISPTRVVTISEKTLQGVYAPLTVNGNIVVDGVVVSCYAVVSDANLAHAVFAPMRGLHYLSQYVPW FLHSTQQENAPQNGVHW YAKMLYNIGSTFLNEKTLYIA* Lottia Protein Model: jg i_Lotgi1_159314_fgenesh2_pg.C_sca_20000054 NR Annotation: Buccalin p recursor [Contains: Buccalin-D; Buccalin-E; Buccalin -F; Buccalin-G; Buccalin-H; Buccalin-A; Buccalin-I; Buccalin-J; Buccalin-K; Buccalin-L; Buccalin-B (BUCb); Buccalin-M; Buccalin gene-predicted acidic peptide A (BGPAP A); Buccalin-N; Buccalin-O; Buccalin -P; Buccalin-Q; Buccalin-R; Buccalin-C; Buccalin-S; Buccalin gene-predicted acid ic peptide B (BGPAP B)] gb|AAB27696.2| buccalin precursor [Aplysia californica]

121

NR E value: 3.00E-66 Ac Annotation: Buccalin precursor [Contains: Buccalin D; Buccalin E; Buccalin F; Buccalin G; B_[0.0]_[43]_APL_all_052305.95.C2 Ac E Value: 4.00E-75 Sequence: MAARKHELVLVLTSVLCFVSSIVGDPNVPSDSQDNSALTQDDFAKRGMDKFGFAGGVGKRGLDKFGFTGQLGKRDMDSFGFAGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFAGQLGKRGLDQYGFTGQLGKRGLDQYGFAGQLGKRGLDHYGFAGQLGKRGLDQYGFAGQLGKRGFDQFGFAGQLGKRGLDHYGFAGQLGKRGLDQLGFTGQLGKRQMDIFGYRGQLGKRQSIDKYSFLGAGIGKRSVKNTAGIKKDDA* Lottia Protein Model: jg i_Lotgi1_163440_fgenesh2_pg.C_sca_39000174 NR Annotation: No match found NR E value: Ac Annotation: BEL-2PROTEIN_[0.749555]_[1]_APLCNSN01-F-043283-501 (antiapoptotic protein) Ac E Value: 1.00E-40 Sequence: MDLYGVVW LLAILQEVTSCFLTEKLPSLEGKEWFTKIRSGGSNDVIAYFDRNSVTFNDVKNTTNGETKW RCFMKIRGRYFLKNVRQGGGYLYKCMGIIVRSESAIQLEWSHVSRLADPALCAEENINLDPW LLISYKTVVNDFTSCPVSGGFDIKHAGYYSRDVRDSRGYDIGCNLMNIPMRLEFDCIAGEGAIFNFRSKNCVPDIHFNIYQNTICVAKWSNKRHHFVLLRTKTGLEFW CARFPVGIENQNETELYLYSDVACLEGEYAEEFVKFMKFDLQRVMYTTICADEYPQCKEGTCNVFSKLECQKTCGLCNPHRPPGICTFPKRMQGTYLLHSKHGNQNVTLEGRTLNIENVGQFDCIVFEDSPLRSTKTYTTMSIFQNGCRPRYTCVRLKRLGPSALRYSLAQNLVW PIKKERIGANICNEDNFRADGDPIRDTFRSYKKTGKPVIKIHPKPRFINCGMNTSYTIRAELPDGSICHGGFYQHCKNETKLRFDFHTCNSFIKPKEDFNCAGIFRTNYW ERAVLIQNVDNLNDIRCLIFNSLRPYQALIVVADECDKMASGLVDSNIRIPIMRLHIRSDVYPCKHIQLPLKDDVITNVVTTTEPIKMNVTLKESKNVNSFSVDLELSSRNSSGSRPFENKLNSLLLLFLCYVVFVKNL* Lottia Protein Model: jg i_Lotgi1_164016_fgenesh2_pg.C_sca_43000014 NR Annotation: chordin [Danio rerio] sp|O57472|CHRD_BRARE Chordin precursor (Protein chordino) gb|AAB93485.1| chordin [Danio rerio] NR E value: 1.00E-119 Ac Annotation: Chordin-likeprotein 1 p recursor (Neuralin) (Ventroptin)_[1.94386E-18]_[6]_APL_all_052305.11591.C1 Ac E Value: 2.00E-24 Sequence: MSPMDLAVCLVLLAATVSSTKFSKIPLKAARENLFGIQPNRPGCILGSKFYGI GERWTPELLPVGEMYCVKCECVPVERKGIIDMKGQTLCKNIKNLCPRPNCLNPVLPKGKCCKVCPDEVNSFEDSFSLGSKSSAPTMISSDSKRRVRNEFVSLLVGKNVRRESVKTSAVAAVYFSIVGRDEIRYSVRYFKLDRPKFLQITDANGNVLFERPIEKKRNGDKKFCGIWTKVPSAYIQYVREERLFAVITASRHFRGLVSGRIMVNRHAKREVFSSVLASPKANGIGGLLSVVYNPRTHLVDYVINLDGVFPGSSEEYFITISKKSKVLHQSKGKASSKTRFIRGSWTLKNKRQSKQLARGRLTIRLTSTNGASVSGDIKPKVTCGVSQAVMSSGNSLEEMKLASSGTAVFELREKGSLDYKIRVTGLTSKVHRIRLEGAVNKKNRRQIIGSVHRNFKADENSFNGW CNGTFKKMTADDLHLFLNNKIFVNVATSENRISELRGRVIALPYHSHFEQVSADPAALGPKTITSNGHAGNAWISMGVGCSLHYDITTTTIAKHHDLTLSLVSPTDIDAYVTIEKISTTNLYEFQQASGSIAVVPESLFRDLDEGSALIQVISGDVILSGNVSVPNTCWQYSQDYDMDILVDEQTSEEVLAANRYKCVYEGKVYENGDSW LPDVNATCNTCSCNKGKIECHRLICPVVECANPIVLDGECCPTCPIETTDQTECKIDGDLRSHPVGSTWHPFLPLMGYAKCVICTCLPGGIYKCERKCPKLDCPASRRIRLNPKDCCQVCAPEEVKPEVVKPLQDVDMKGACSFKDQIYANGAKWHPRVIPFGYMKCIDCSCNNGTPSCSRPKCPKLSCSRKIREDGACCDTCADSDGEEIVEEKKSDSCIFGGKKYDDGETFQPKTSSLSSCATCTCRKGSMKCSLTCPEKCKDPKHSNDPCCKHC* Lottia Protein Model: jg i_Lotgi1_164520_fgenesh2_pg.C_sca_45000185

122

NR Annotation: PREDICTED: similar to filaggrin 2 [Rattus norvegicus] (molecular regulat ion of epidermal terminal differentiat ion) NR E value: 5.00E-37 Ac Annotation: A-agglutin inattachment subunit precursor_[2.05518E-9]_[1]_APLCNSN02-F-021300-501 Ac E Value: 2.00E-11 Sequence: MIKLTLSILDIVQIVKVTFSILDTVQIVKLTLSILDIVQMIKLTLSILDIVQIVKVTFSILDIVQIVKLTLSILDTVQIVKLTLSILDIVQIVKLTLSILDTVQIVKLTLSILDTVQIIKLTLSILDIVQIVKLTLSILDTVQIVKLTLSILDTVQIVKLTLSILDTVQIVKLTLSILDTVQIVKVTFSILDTVQIVKLTLSILDTVQIVKLTLSILDTVQIVKLTFSIFDTVQIVKLTLSILDIVQIVKVTFSILDTVQIVKLTLSILDIVQIVKLTFSIFDTVQIVKLTLSILDIVQIVKLTLSIHVVC* Lottia Protein Model: jgi_Lotgi1_168619_fgenesh2_pg.C_sca_77000042 NR Annotation: crossveinless [Saccoglossus kowalevskii] (a new family of twisted-gastrulation-like modulators of bmp signaling) NR E value: 1.00E-148 Ac Annotation: VonWillebrand factor precursor (vWF) [Contains: Von Willebrand antigen II]_[0.0]_[6]_APL_all_052305.10450.C1 Ac E Value: 1.00E-44 Sequence: MYYTELSILAKCIALCILTCFPILIQAQLTGTVTRCYNEGGEVKIQGITTDPCISCYCMDGYVQCDKKKCDNLDGCHAILFDGPKRCCDTCKGCRLNGVKHESGKSWFDKRDRCTSYSCKAGVITKSKVQCHVPCSNPIKRKRQCCPSCKGCHFDGADRKNGEKFALASDPCVECKCRKGSVTCMKRACPVLNCPDEVIYQPKGECCPKCKGMRKIFDFPGACYFAKRVYVDGFSFEPKSETRCTCTKGTVICEKATCPPVACPVEERVQKGSCQVCEPKRNCLYDGKIHMHRSKWQPRMCTQCSCQNGVTYCQRERCNNSLSCPNGYKLQFQPGECCPKCVEHDAVCRVFGDPHYRTFDGKMYNFQGTCKYMLSQDCQGKDFTIKVKNGVRLSSGFAWTQMVVVLMGDTRISFRQNLLIKINRRRVQLPYTIPGKFSIRREGHSVTFRADIGLKVVW DGDSFLEVTASRKYKNRLCGLCGNYNGLETDDLIGRKGKNYLRGEEFGNSW RIGSKKACKTQPKVKNLQSICDKDFKAKVRANKECSVLYSRAFSSCRRVVDVTPYVTSCVTDMCDCPHGKKCSCESIQAYAHECKRAGHKVKW EKVSNCKAPQKNCPKGAYYSLCAPACPKTCSSNKPDGSCSKKCSPGCVCQNGAVLYKNRCISPEKCPKT* Lottia Protein Model: jgi_Lotgi1_179503_fgenesh2_pm.C_sca_71000002 NR Annotation: Wnt7 [Euprymna scolopes] NR E value: 1.00E-122 Ac Annotation: Wnt-16protein precursor_[8.48178E-30]_[10]_APL_all_052305.8921.C1 Ac E Value: 7.00E-23 Sequence: MTVSPTVVWAAFSMALSSVVALGANIICNKIPGMAPKQRAICRSRPDAIVSIGEGAKLGLTECQYQFRFMRWNCSTLDSNDSMFGYESLGGTKEAAFIYAMTSAGVSYAITQSCGLGSLPNCGCDKDKGDGKLAPQGWKWGGCSADIKHGLRLARKFMDAREIAQNARSLMNLHNNRAGRKAVKDNMGTDCKCHGVSGSCTMKTCWTTLPPFRKIGDSLKKRYKKSKIVVPYLGRRARTAVTLILKRAKRPHRKPRRSHLVYLDKSPNYCDFDGKTGSLGTVGRKCNRTTKDTDGCDLMCCGRGYNTHQYTRTWQCNCKFHW CCYVNCNKCSERTEEYTCK* Lottia Protein Model: jgi_Lotgi1_186451_estExt_Genewise1.C_sca_160091 NR Annotation: PREDICTED: similar to out at first [Nasonia vitripennis] (Vital for proper neuronal development and hatching) NR E value: 7.00E-69 Ac Annotation: Out at first protein_[0.0]_[4]_APL_all_052305.9349.C2 Ac E Value: 5.00E-90 Sequence: MSMSLIPSLSYSIFVCVLIKICHTQLVVNVKNGGGDILVESIQSNVSSDTITLEYPSSDGSLITQFIDFKSEAQIF

123

RAYVPGEEERGQPPGSVQVLCFITRFSKNDFISSDAMSKLRQKNPTAIRTPEEERPQEVHSFDLSVDLNKSHVISPHVFNICREAKESTFFNEDDLKTISRSLSKDYNTMMSAMIKLAPTKYGKCSDTSDIFKPCTCRYDACVGWYPCGLKYCRGKDSSGKVVSYRCGIKTCKRCLAFDHFVKQKFLCLWN Lottia Protein Model: jgi_Lotgi1_188920_estExt_Genewise1.C_sca_260301 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO37057.1| p redicted protein [Nematostella vectensis] NR E value: 1.00E-109 Ac Annotation: Multip leEGF-like-domain protein 3 precursor (Mult iple ep idermal growth factor-_[3.47103E-24]_[3]_APL_all_052305.12391.C1 Ac E Value: 1.00E-114 Sequence: MFLEVFNISATVLCILFLIGHRKCDTIQNSKHHAELMMDLLQPYKNYHGQRLKKSNRHIRECQEIKYGNVTHSKVKVKPDTNISYPLVEVKHLTKEIGSYYYKNPVTIRYQRVDFPLNTLSVLEPGEPGTCREGHGTRSITSKTALSGNCIVGVNGGFFNTTTGACLGNIVSNGRLVQDSQGLQNAHFGITEDGYIYVGYLSELDLITQQFKQLLGGVLW LIRDGEKYIDKSKQMECPDVEETGTIETFIEVQSARSA VGHDKDGRVIMVQVDGKTNARGVNLHEFTDILLELGVVNAINLDGGGSTTTVINHTLVNYPSDKCADGTFSCEREISTILCVHEPECQPNDCNQHGHCVLGKCVCDGYW VGSDCSSLSCPFSCSHHGTCTKDGCICDDGW YGINCSTPCKPGYYGSNCAHQCYCLNHGTCNSQTGHCVCSPGFTGKYCESLCEFGYYGDKCNNLCTCDNGCFCHHVTGSCNLS Lottia Protein Model: jgi_Lotgi1_205842_estExt_fgenesh2_pm.C_sca_70027 NR Annotation: BMP2/4 [Patella vulgata] NR E value: 1.00E-159 Ac Annotation: Bone morphogenetic protein 4 p recursor (BMP-4)_[8.29921E-13]_[3]_APL_all_052305.15764.C1 Ac E Value: 7.00E-27 Sequence: MIADFYRSVVLLLLVLVVNYTSSLSTNTLHPRKHGNEKVLAAVESNLLGLFGLKSRPVPGRRTPIPDYMLDLYKRLDSEPDFISPFAESKGKGIVEANTVRSFYHSDINFVKDCDERTCARIWFNISTIPFAEIMAASELRVFKDVYNHFKLTDANSLSGDRIKASLFKHRLEIHEIMRVSPDDSECISRLIDTKIVDTRNSSWESFDVHPAVLKWRRRPQYNYGLEVRIVSKSPWISTNSHVRLRRSAHMDEKNWQIQRPILVTYTHDGRNPNSRIKRASARNRRRKSRKRRRRKKKGNKNECKRHALYVDFGDVGW NDWIVAPTGYNAYFCRGECPFPMGQHLNSTHHAVMQTLVHSVNPSAVPKACCVPSELSAISMLYLDEW DKVVLKNYQDMVVEGCGCR* Lottia Protein Model: jgi_Lotgi1_208417_estExt_Genewise1Plus.C_sca_10710 NR Annotation: fasciclin- like protein NR E value: 5e-178 Ac Annotation: Transforming growth factor-beta induced protein IG-H3 precursor (Beta IG-H3) (K_[2.29414E-23]_[11]_APL_all_052305.8904.C1 Ac E Value: 2.00E-61 Sequence: MSRNLWIFLLILLSIIVIASSRRSRGRW YRGRSGRRSLADRLRDRGTKPSNHRPRQRHGW GRW GSGW GAGQDHRTRVEEELDDLFRRIKDRRYGFNFEDKFW LDFGWNSRNEPKPWWEGPNVCKDEKESIENTSISDTGPISRHFSFKNQVCDQSETSYKCTTKTGIFMKQKTVTEVYECCPGFTRKDEMFGCPTEFKLDGLVKTARNLGLTEFLRATESVSLTDDLEKGNFTVFAPVNKAFEDEKNILPELETILPKDMPSVVMVSKPLTDLVIADMSNVLLGHLVYDTLKTSGLNDEQLIETASPFDSLLRVNFFSRPEKLITVNCARILSADNQATNGIIHTVDRIIKPVTDSIVDIISQNPNLSYLKTALARADLVKALRGTGQYTLFAPTDAAFEKLEPSIRDRLIKGEKSCLEKVLKNHLLTSVICSTVIQGTVTTTNLLNKYLNLTRTEDDKLFVEGSQMVGRDIMATNGVIHVIEDVLVPDQALGLIDVAEKNNLTKFVELLKETGMFKGITKMENLTLFIPSNNAFEKLEEKVSLEELKKDKKKVEDIVMYHVVPEVVTCSRLHNNYKLSTENERNIRMNRYSTFPFRHHSIQTAQCAPIIKENVPACNGVINIVNEVLLPPVGNVVDNLASKKKFSTLVRLMKKSGLADMLQEEGPFTMFAPTNEAFDDLYEEDLEKIEDNPEVLKKVLQNHIVQEFVCCASISMVRSWFDVASVRTLSGVRFPVKERHGTIQFNHAVVTECDNTATNGVVHAIDKVTLSPERPFWKRDFYHW*

124

Weak Annotation

Lottia Protein Model: jg i_Lotgi1_110650_e_gw1.12.27.1 NR Annotation: unnamed protein product [Tetraodon nigrovirid is] NR E value: 1.00E-32 Ac Annotation: Slit homolog 2 protein precursor (Slit-2)_[0.0]_[4]_APL_all_052305.13937.C1 (axon guidance) Ac E Value: 3.00E-12 Sequence: MKIILVLLCFCHLIYSAKTCPSNCQCHLTDKAVNCYKRELQFVPANIPLDTEILDLSSNDIANIDPTMFHNLTKLTDLVLNDNKISKLDPRTFQNLHKLEVIKLSQNFLSTIPEGLFDNLPIMVPKLMELNLSQNKIVKIENYVFRPLSMLSTVSLNDNPLKNADRLLSKNRRLSYIDMSECQLTTVPRGLPDSIEHLKLIKNNITKIKPKDFRNKNYLKILALDENEIEFIAPNSFKSLQKLIQLW LNGNRLRKFPSPISPSVTNVYLAKNNITKLSAADF Lottia Protein Model: jg i_Lotgi1_113861_e_gw1.18.363.1 NR Annotation: PREDICTED: similar to tartan CG11280-PA [Apis mellifera] NR E value: 5.00E-27 Ac Annotation: Insulin-like growth factor binding protein complex acid lab ile chain precursor _[1.10885E-21]_[2]_APL_all_052305.14739.C1 Ac E Value: 5.00E-18 Sequence: MVKPPTQNTRWWIIVWILLFQKYTCEPEVTCPHGCKCDVDVVNCLHSNLTSVPVGLSNGDRLQELKLGRNKITEIKLELLEYKELRLLDLISNNISTIDGSAFRTLYKLETLFLSRNFITKTDKNTFRNLTSLRFLDLSYNNLRCLKIGNFEDLQSLVNLDLSRNQISLIEAESFRCLENLEILKLPRNQLDQFPW KIFQNLTSLKMLVLDDNLLTSLPISAFSNLINLEYLSLTGNRLHELLPSSFMMSDEGAISLEQLYLRGTDLDTIPVKLFAKLKKLTVLDLSKTQIVSVKNENLNGLSSLESFIMTSSNNLTYIEKGVFQDLHSLRKIDISGNTLLSEIQFTTSSLRNLSLFDLSANSL Lottia Protein Model: jg i_Lotgi1_137329_e_gw1.113.40.1 NR Annotation: insulin-like growth factor b inding protein, acid labile subunit [Sus scrofa] gb|ABE73450.1| acid-labile subunit [Sus scrofa] gb|ABE73451.1| acid-lab ile subunit [Sus scrofa] NR E value: 1.00E-21 Ac Annotation: Slitprotein precursor_[2.64913E-12]_[2]_APL_all_052305.8468.C1 Ac E Value: 5.00E-13 Sequence: MSFVQLLPFFLFLTISQSSSQRWPLSGTCYPCSCDINQKGFGRVKYVNCSNLALTRIPRNLPLDLHTLDFSNNRILPQEIQQLCTFNSMQYVALSYNSLDSIPPEIFKDLENLHTLVLHGISSIPTTNIFEDLFNLEYLDINDCHVNQIPDNWFRKLYSLKTLKLRQTGIRSIEPGVLDGLLNLQELDLSHNVMRTAQTESFKPIVQSIRRINFRGNMFKTIGDHLFEKMYNLVELDLSDNKLESIHKRSFLDLRQLINLDIRDNKLSNLPGGLFKNLRNLQTLNLAMNTFNNFPETLFKIGYLLKLDLSYNRIRRLPDSFVHSFPYLEFLNVDKNPLHCDCKSLDVKFYQPNLVII Lottia Protein Model: jg i_Lotgi1_152641_fgenesh2_pg.C_sca_2000061 NR Annotation: interleukin 17 [Crassostrea gigas] NR E value: 5.00E-07 Ac Annotation: CNSN01-F-034307-501 Ac E Value: 1.00E-06 Sequence: MVLMKVFICALLVNMVYSFTLIKNIEHILERERRSDECAVPNNIDELFTNLNSHVNRVEFLIAVNATHQPNAQGLIPPAAGDQTDTECPKEYTGKDTSNSGIW QRSTCPWFYSERNYGAEYYPSKVYNAACKCVDCLYSKSNSETGCSRIYRNINILKKTGCSNGFYTYEQRSISVSVGCTCAKLQANIG*

125

Lottia Protein Model: jg i_Lotgi1_157019_fgenesh2_pg.C_sca_12000330 NR Annotation: No match found NR E value: Ac Annotation: Chordin-likeprotein 2 p recursor (Chord in-related protein 2) (Breast tumor nove_[0.235318]_[1]_L7ALL-ETFK5Z408EYR8G_257 Ac E Value: 2.00E-08 Sequence: MFSLVLLVVTPWLVFCMPPPPPAMCTCSDGTQVPPGGTHSISDCHSCYCDENNSEAQEIIAACLIVPCVDSVKMPGQCCPTCPNGENCRAEFKDDNGVERSEIIKVSDGVVKFNSTECQCDYQNYNGEPKANCVQVLSIEPVIAEKSTP* Lottia Protein Model: jg i_Lotgi1_167943_fgenesh2_pg.C_sca_71000005 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO34356.1| p redicted protein [Nematostella vectensis] NR E value: 1.00E-21 Ac Annotation: Fulicin precursor [Contains: Fu licin; Fucilin gene-related peptide 1 (FGRP-1); _[0.00557519]_[20]_APL_all_052305.37.C1 Ac E Value: 2.00E-16 Sequence: MKSILVTSVVVVAGTLLTLVQALGADKFAEPATQKHSKIPTAALSHSGDTLLNTDAHQTNPSNIWKSKSKFDLDALPVVNRQDVQSHYAQHRRSYDTPMFLGKRDLKNLSLRFKRATDLEDLDATEFSDDFDKRGVDIPQFIGRRRYDTPQFIGRRGYGVPAFVGKRAFRAPAFIGKKAFGAPAFIGKKAFDAPSFVGKRKLTAPSFIGKRGLDVPAFVGRRAYAPPQFIGRRGMDVPAFVGKREFQTPMFLGKRAFSAPSFVGKRGLDAPTFIGKRKEESFRDLLATLQAYREYRRLMQTDKRFDAPLFVGKRSDYENDNLAAETLSRRFQCIPQW VFRMRLNVVDLNMSFIGGYKQNYFSLNILDDVQTKILLPLDDLHKVDSSKMDVPTVQLWDADMPRPNGARFLKLDPAHALGFLGQEASPVSKRYLEFVGKRDNDKRYAEFVGKRAGFGEKRYSRGSLSEYLSKRLRIRRPEFVGKRSNQKRYLEFIGR* Lottia Protein Model: jg i_Lotgi1_168377_fgenesh2_pg.C_sca_74000087 NR Annotation: TBL-1 NR E value: 2.00E-07 Ac Annotation: Dorsal-ventralpatterning tolloid protein precursor (Mini fin protein)_[0.0204183]_[9]_APL_all_052305.10078.C3 Ac E Value: 7.00E-14 Sequence: MLPHITQCVILFITFLCDISVLLPSRDGGRGLTYAQLVPHRTSNDVYMDPCKAAGFMGDIALSEEEFKRERKYLQQMNNTMFELQVEDPRTTYIGRKPSKYSGQPENTLKKLPMSELDVKKKKYLRKILKEKKQNLKSLCKKGFQTVVYTRQVPVGFKFISPFLYLLDLNIL* Lottia Protein Model: jgi_Lotgi1_168508_fgenesh2_pg.C_sca_76000035 NR Annotation: ependymin-related protein precursor; EpenHg precursor [Holothuria glaberrima] NR E value: 3.00E-10 Ac Annotation: Mammalianependymin related protein-1 p recursor (MERP-1) (UCC1 protein)_[0.0126998]_[10]_APL_all_052305.1963.C1 Ac E Value: 5.00E-19 Sequence: MMKYCLVVAVVVVGVVIGEPSKPGCCTPSQWEGQQGSTIGIVDGGIPSVRTSMVRISYDEKNQRIAYVTSESTPQGEKNYKIIYDYSRKFQFIVDLDTKHCTVSSLTDPFMKLCLPVDAQPKGKFYYGIDGNNLPAMAFSLNVNGTMITVVTTADNCIPIAEVLVDGQAQGVSQMHASGFVNLTSGIKDPSVFIPPGTCTQ

126

Lottia Protein Model: jgi_Lotgi1_169325_fgenesh2_pg.C_sca_84000054 NR Annotation: Insulin precursor [Contains: Insulin B chain; Insulin B chain'; Insulin A chain] gb|AAF80383.1|AF160192_1 insulin precursor [Aplysia californica] NR E value: 4.00E-13 Ac Annotation: CNSN01-F-005950-501 (unknown) Ac E Value: 2.00E-11 Sequence: MEVTCKCPLVLLGVLFLNFGTVLTHLEWTCTLETKRESPRGVCGQRLPEVLSMVCKRYGGYRDTWFRKRNGEGTNSRLGNIILGKRDAFSYLGKRGQSYGEQGITCECCYHSCSFRELRQYCRNSQQRISIKK*

127

Object 2-3. Controversial predicted neurosecretory products.

All software programs are inherently flawed in that they can only use those parameters

they are programmed with to make predictions about whether a protein may or may not be a

secreted signaling molecules. Due to these flaws, our final list of predicted secreted signaling

molecules was manually screened for conserved motifs, signal peptides, and annotated to make

the most conservative list of predicted neurosecretory products. Here, we show those transcripts

predicted to be secretory molecules due to targeting to the classical signaling cascade but are

unlikely to be secreted outside of the cell. Some of these predicted secretory molecules may be

part of the secretory apparatus but do not encode for signaling peptides.

Furthermore, many of these predicted molecules have motifs of non-secretory products but

may be secretory in our model species. Further experimentation is needed to determine the

functional role of these products, so we have decided to leave these products in this supplemental

section to provide a complete unbiased review of all predicted products for future researchers.

Found by Homology Search

7B2 secretory granule neuroendocrine protein+


Reference: N/A CDS: ATGAACATCCTTCTCCTCGCCGCCACCCTTGTGGGTGTGACCTTGGCCAGCTATGACCCCTACGTAGACATGGCCCAGCTGTACCGGATGCAGCTTCTGGCCAACGCCTTTGACGACTACCTGCCTGAGAGCCAGCTTCTGGACAGCCGCAGCGAGGAGCCGTACTGGCCCGAGCTAGAGGACGTGGCTGAGCCGCAGGACGACAAGGATGCCATCTATAACGACAGGTTCTACAGCGGGGCTCATCTCAGAGATCAGGAGCATTTGGAGCATAGCGCTCTGCACGGTTATCA GTCGGTGTCTGGCGGTGCATCAGAAGTCCCCCCTAACCCCAAACAGGTCAAGTCGGACAAACAGCTGCCTGAGTACTGTAACCCACCCAACCCCTGCCCTGTGGGCAAGACAGCCAAGGACAACTGTGTAGAGAACTTCGACAACAGCGCGGAGAACAACGAGCGCCTATTGTCCCAACAAGACTGTCCCTGCGACACCGAGCACATGTTCTCCTGCCCCGCCGGAAGCCAGACGGTGTCCTCCAAGGCCCAGAGCTCGGGCAACCAGCAGATGGCGCTCAACAAGGTCATGGACGAGATCGCCAAGATGGAGCATGATGGGGAGAGCTTGGAAAACAACCCCACAATGTCGGAGACGCGGAAAAGAGTGACGCTGGTGGCAAAGAAGTCTCCACATATTATTCACAAACGATCTGAGCAATCAGACCACAGCAATCCTTTCCTGCAAGGAGCTCCTGTAGCTATTGCTGCTAAGAAAGACCCCAATACCGCACAGAGGGTCATTCCCCAGTGGGCCAGATACGACCAGCCCTTGCGGTGA Protein: MNILLLAATLVGVTLASYDPYVDMAQLYRMQLLANAFDDYLPESQLLDSRSEEPYWPELEDVAEPQDDKDAIYNDRFYSGAHLRDQEHLEHSALHGYQSVSGGASEVPPNPKQVKSDKQLPEYCNPPNPCPVGKTAKDN

128

CVENFDNSAENNERLLSQQDCPCDTEHMFSCPAGSQTVSSKAQSSGNQQMALNKVMDEIAKMEHDGESLENNPTMSETRKRVTLVAKKSPHIIHKRSEQSDHSNPFLQGAPVAIAAKKDPNTAQRVIPQWARYDQPLR Acyl-CoA-binding-0R protein Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: CTTCTGATAACGGGCAAAGAACANGAAAGCTCAGAACTTCTGACAAACTGTCATCGTTCAGTTCAAACTCTAGTAGTAGTACAAATTCCGGTCAAAAGCTTGAGAGCCTTTCTAAACCAATTCCCAAAAGAACATCATTTGCCTTGAACAACAATGTGATCAAACCGGGCCCCCGATCAAACCACCGAAGAAAACGCCCCCTGCTGTCCCGGTGCGCAGTCCCAACACAGGGCTCACCCCAGGNCCTCCCACTGACTTGCCCTCAACACGGCTTATAATACTCGCAAAATTGGCAAGAAGCTGGGTATCCAGCTCAAGAAAGGTCCTTTAGGCCTGGGATTTAGTGTGACATCTCGGGACAATCAGACAGATGGGAATACGCCCATTTACATCCGGAATATTCTGCCCAAGGGGGCTGCTGTACAGGATGGACAACTCAAGCCTGGGGACAGACTCTTGGAGGTGAATGGTGTAGAGATGACTGGCAACACTCAGGCTGAGGCCGTTTCCTTCCTTCGCATG Acyl-CoA-binding-1F protein Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: GACACACACACGCACACACACACACACACACCGGTAGGCCTCTAGACTAGAACTGATCTATCATTCGTCTCACCTGAAGGCCTTGTTGCACGGGACACGCCTTTTCACAGACCACTTGCTTTGTTGAGTTCATTGTGTTAACGTAAACGGTTAACTACACAAATCCATCGACATGGCAGACTTGGAAACTAAATTCAAGACAGCTGCTGATGAGGTGACAAAGCTGACCTCAGAGCCCTCCAATGAGGAAAAACTTGCTATTTATGGTCTCTTCAAACAGGGGACTGTCGGGGATTGCAACACAGAGAGGCCAGGAATGTTTGACATGAAAGGAAAGGCCAAGTGGGATGCCTGGAATGCATTGAAAGGCAAATCAAAAGAGGACGCACAGAATGAATACTGTTCCAAAGTTGAAGAACTGAAGGGGAAATACAACTAACTGACATTCCTTTCCTAGTTTTCTTCACCTTTTCAATTTCATATCTCCATAGTGTTTGTGTTGTCAAGTTATCTTGTTGGATGATCACGTCATGTGTGCAGTGGCCCGCTGAGAAAATCTGGCACAGACAAACAACCTGGGGAACAACGAAGTTTTTTGGACTTCAGAGAAACTGTTAACATGACCAACTAATTAGGAATTTTTTGTCTTAATGAAGCTAGACAAGTATTGAGCCTGTGAAGACACATCCATATATATTTTATCTCGCTTGTCAGAAAAAGACTACTTGTTGATACATTCGCTTATTTGTAGAGTCAGAGTTAGTCTTGTTACGTGAAGTTATGTGAGCATAATGGGTATTTAGTATTCTCATCCACTGCTGTCTGCTCAAGGTAAGAGATCGATCATGAAATGACTGCCTGTCAAATTATTGCTTTTGTGCTCATACTGACTTTTTGCCATGACTTTTATGACCAAAAAAAAGTACAGCAGGAGGCTTAACGTAACATTTCATTTTGGAGCATTATTCTTTTGTTAGCATACTTTT Anaphylatoxin-like 1 (Complement component 3) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: CAAATGGCCTGGACACGCAAACGCCCCTTTTGGCAGATATACCCACGGCAAGGAAGGACCATGTGGTGATGGAGTCAGGTAGTGTCGTGTTTAGTGTCAGTTGCCCGGTACTGCCGAGATTATGTTCCTCAAAAAGCCATGACTCCGGGAAATATTTCCTCACCGTTTGAGGTGGAGCTTCTGCCACAAACAGTCCTTTATCTGTCTTTTTAACATGATCCCTGAACCCACTAGGAATTTGGGAGATAAATCCTCCAGGTCCTAATGGTCCAGTTGGTCCAACTGGACCTATGTTGGCTCCCCCTTTACGAATCGCCACTGTTCCTTCAAAGCGCCCTATGATGGCGTTAGAATCTACGTTGTAAGGGGCGCTTGAGTCAACCAGGCGATGAATTAGACCAGAATTCTTGAATATTTCTGTTGCGTCCGATCCGTCTCCACTGTCTGAACCTTGGTCATGACGCACAAATTGGGAAGACATCGATTCTCTGGACAGAGTGACGTCCTTGTTCACCAGCAGCACAGCTTTGTCCACAGCCACCATGCCCACTTTCATATTGCGGCCACCGATGACATTTAGCTTGTACCCATCCTTGGGCTTCACGCTCAGACTCCCGCCAAACATACCACCGGGCTTTTGGACTATGACCTCCTCCAGACACACGTCTGGGGTGTCGACCAGCAG

129

AGAGTCCATAGCAATCTCATCGTCCACCATAAAATACGCTACCACTCTCATACTAGGGGTGAGTTCGCCGTGGAGTCGTTTCGGCAGACTCACAAGAGACCGTCCGTCCAGACTACGCATGGTGCTGGTAGTGTAGAGTACCTGTCCTCTGGAGATTACCAGTACTGTAATCGTGGTAAAGTCTTCTCCCACACCATACCAGTTGTCCTCAGGAGGTGTGTATCTCAGTAGGATCACCCAATCTTGAAATTTCTTGACATCTCCGTCCATGGCTATGTGCAGGTATTGGGAGTGGGCGAGTTAAATTTTCGCACTGAGAAGGTCTTAAGCCTTTGTGTTTCATGTCGGTGACCTGAG Apolipophorin precursor protein Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: AACTACGAACTGTCTGTTTTCTCGGAGAGATACGGAGCAGGAGCCATGCTGAGAGCATTGTTGTGTTTTCTTCATCTTCTTTCATTCCCAGATCTGCCATGCTGAACCTAACAGTGGACATGCTGGGTCAGTCGGTCAACGTGTTGGAAGTGGGAGGTCGTGTACAAGGCCTGGAGCTGTTGCTACAGAAGTACATGGGCAGCTTTTTCAGCGGCGAAGAGAACAAAGGGAAGAGTGGCCCCTCCTCAAGCTGCGTCCGTCAACAGAAGATGGACAAAATGAAGGATAAGTTTGCGGTGTCAAAAGATGATTTGGAGGCCTCTTTCTACGTGAGGATGTTTGGTAATGAGCTGGGCTTTGAGCATCTGAGTGGAGAAGAACTGATGAACATCAAGGACAAGTTCAACCTCAAGACGATCATGAAAACTTTGGCAAAGGGAGACGATATCTCGTTCACCAAGAGTGCCATGTTCATGGACTCCAGCGTGGCTGTGCCAACTATTGCAGGTATGGCTCTGCGTCTCAGTGTGAATGGCACTGCGACTGTTGAACTTGTTGCCAATGGAAAGATTGACATGAGAAAGATCTCCCGCAAAAACATGGCTGCCAATTTCAATTTCAGGCCAAGTGCTGCTGTTCAGCTGTCCGGTTTGATGAGTGTGGAAGCGGGAGCGGCCCGGTCAGGACTGCGAATGACCACTACCCTGCACA CTACCAGTGCCACCACAGGTAGCTTGGATTTGACCAATGGTCAGGTGGTCTCGGTAGACTTTGACACTCCTGTGGAGAAGATGGGGCTTCTTGATGTCAAGTCGGAATTCTTCATCCTTCACACTGAAGCAGAAAAGAGTGTTGAGATGAAACAGAACAGCCTCGTGAGTAAGCGTACATGCTCTCCCGACATGCTGCTGAAGGTCACCGGCTACGAGGTGTGCGGAGAGTTTTCTTTCATGAGGCCGGCCACGCCAGACACCAGTCCCTATTTCCCCCTTACTGGGCCCGTGAAGTTGAGCCTGACCTTGGACAAGAAAGATGCGCCGAGTGGGTTTAAGCTTGTGGCAAAACAAGTTCAGAATACCGACACCTCTGAGTTCCACTTGTTAGTAGACACCCCGAGGTCGAATCAGGACAGAAAACTTACTGTTGACTACTTTCTCGACCAGCGTGCCAAGTCGCTGGAAGCAGAACTTGCCACCCCGTGGAAGAAAATGTTGGTTAAAGGA GCATACACAGTCGGAGATGACACACGCCTTCTTTCAGCCTCTGTGGTGGTTGATGGACGCGAATATGAACTGCTCACGCAGTTAGACAAGGATGCAAAAGGGGTCAAAGGTCAAATGGACACCA Beta-Amyloid precursor protein (appear to be the main constituent of amyloid plaques in the brains of Alzheimer's disease patients )+


Reference: Brough, T., A.B. Kohn, L.L. Moroz (2007) unpublished. CDS: ATGGGGCTCGTTACAAATTGCGCTATTTTCTTAGGATTACTCCAGGTGTTGTACGCCGCCAGTGTGGAGGACAAGTATGAGCCGATGGTGGCCTTCATCTGTGAGAGGCCGGCTATGCACCGGGGGGTCAATGGATGGATCGCTGACAAGTCTACGGACTGCCTCAATAGGATGGAAGATATCCTCTCTTACTGCCAGAGGATGTACCCTGACCACAACATCACCAACGTAGTGGAGGCCTCCTACCTGGTCACCATCAAGGACTGGCCCATGACCTCTGCTGACCGCATGCACCCGCACCGTGTCCGCCCCTTCAGATGTCTCGTGGGTGGCTTCCAGTCGGACGCTCTCCTAGTCCCCCAGCACTGTGTGTTTGACCACCGCCATGACCAGCGCGTGTGTCAGGGCTTCTCTCACTGGAACGTGGTGGCGGA CGAGTCCTGCCGCTCCAAGGACATGCACCTGGAGAGCTTTGGCATGCTGCTCAACTGCAAGCTGGGCATGTTTAGCGGAGTGGAGTACGTCTGCTGTCCCAACCAGAACAAGCCCAACTACCACCCGCCACAGACCGACGACAAGCCTGACGACTGGCATGACAGCAGCGCCGAGGAGGAGGATGAGGAAGAGGAGAAGGAGGGCGAGGATTCGAACCCCACCGCCCCACAGAGCACATCCAGCGAGGAGAGCAGCGACTCTGGCGCCACCAGCAACAGCGACAGCGCGGTGACCATCAACGAAGACGACCTTGAGGAGGACGTGATGGACTTCTACGAGGCGTATCTGCGTGGCCAGGAGTTCCCCAGCAAGTACAACAACGAACACAAGAAGTTCCTGGCCGCCAAGGATCGCATGAAGAAGAACCAGCAGCACAAGACTACCAAGCTTCTGCAACAATGGCAGGTGGCCAGGGACCATGTGGACGAGGTCAGGAAGGTGGATACCAAGAAGGCCGACAGTCTGAGCGGAGAGATCGCTTCGAGGTTCCAGAAGCTGTACGCCTCGTACGAGCAGGAAGACGCGGCAGAGAAGCAGCAGCTGATGGCTCTGCACCAGCAACACGTACAGGCCGCCCTCAACGAGAG

http://en.wikipedia.org/wiki/Amyloid�

http://en.wikipedia.org/wiki/Brain�

http://en.wikipedia.org/wiki/Alzheimer%27s_disease�

130

GAAGCGGGACGCCATGGACAACTACATGAAGGCTTTGGAGAAGGGAGATGTGGAGAAGATCATCAAAGACCTGCGTAGCTACTCAAGGCGGAGGAGAAAGGACCGCATGCACACTGTGAA CCACTTCGAGCACGTCAAGTACTCCAACGCTCGCGAGGCGCTGCGCATCCACCCGTTCATCATCAACCACCTGCGTCTCTCAGAGCAGAGGATCGACCAGGCCCTCGAGATGTTGTCCCGCTACCCAGACATCGAGGTCCAAGTCAGACCTGAAGTTGAGGAGTTCATGAAGCGTTTCGACGCCATCGCCAACAGCATCAAGAACGTGGTGCTGCCCCTGCCCAAGGTGGAAGAGGAGCCGCAGACCAAGTCGGCCTCGCAGGAAGCTCCACAAGACGCCAGCGACAGCGTGAGCCCTGACGACGACATCAGAATAGACGACGCGGGCGACTTTGACCTTAGCAGCGAGCAGGTGGATGAGGAAGGCGACGACGTCTCGGAGGACGAGCACGACTACGAGAGAGGCAGCCAGTTCATCGCTCACCGCATGGACGACAAGATGCACGTGCGACAGGGCTTTGCCGAGTCGGCCGCAACAAGCAGCCAAATGGGCAGCACCATCGGCATCGCGCTGGGAGGTGTCAGCGTGTTCGTCATCATCGTGGTTGCTGTGTTTATGGTGAAGCGCAAGAACCGCAACCAGTACCCGTCCCCAGGATACGTGGAGGTTGACCCATCGGCCTCACCGGAGGAGCGTCATGTCGCCAACATGCAGATGAACGGATACGAGAACCCCACGTACAAATACTTTGAG Protein: MGLVTNCAIFLGLLQVLYAASVEDKYEPMVAFICERPAMHRGVNGWIADKSTDCLNRMEDILSYCQRMYPDHNITNVVEASYLVTIKDWPMTSADRMHPHRVRPFRCLVGGFQSDALLVPQHCVFDHRHDQRVCQGFSHWNVVADESCRSKDMHLESFGMLLNCKLGMFSGVEYVCCPNQNKPNYHPPQTDDKPDDWHDSSAEEEDEEEEKEGEDSNPTAPQSTSSEESSDSGATSNSDSAVTINEDDLEEDVMDFYEAYLRGQEFPSKYNNEHKKFLAAKDRMKKNQQHKTTKLLQQWQVARDHVDEVRKVDTKKADSLSGEIASRFQKLYASYEQEDAAEKQQLMALHQQHVQAALNERKRDAMDNYMKALEKGDVEKIIKDLRSYSRRRRKDRMHTVNHFEHVKYSNAREALRIHPFIINHLRLSEQRIDQALEMLSRYPDIEVQVRPEVEEFMKRFDAIANSIKNVVLPLPKVEEEPQTKSASQEAPQDASDSVSPDDDIRIDDAGDFDLSSEQVDEEGDDVSEDEHDYERGSQFIAHRMDDKMHVRQGFAESAATSSQMGSTIGIALGGVSVFVIIVVAVFMVKRKNRNQYPSPGYVEVDPSASPEERHVANMQMNGYENPTYKYF Intersectin-2 (involved in endocytosis) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: TGAAAGACCTGGTGATCCTCGCTGTCCTCTCTCCTCCTTCCTGAAGTATGTGACGCACTTGCACCCAATGACAGATGCCTTCTGGCAGCGGCCAAAGCGTAACGTCTCACAGTCAGACTACGTGTGGTACGACCACACCCCCCT Laminin beta-1 chain precursor (extracellular matrix glycoproteins, implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: GGCTCGAGCTGTGACCAGTCTGTTGACAACTACTGGAACCTGCAGAAGGACAACCCTCTTGGATGTCAAGCTTGTACGTGCAACCCAGATGGCACTATTCCAGACCTAGGATGTGATGATGAGACCGGCCTTTGCAGATGCAAGCGTTATGTCACTGGCAAAGATTGTGATGAGTGCTATCCTGGCTTCTACGGTCTGAGTGCTGATGACCGCTACGGCTGCAAGTCCTGTGACTGTGATATTGGCGGAGCTGTCCGCTACACCTGTGACCAGGCTACTGGCCAGTGCGAGTGCCGGCATCACATCCGTGGAATGCGCTGTACTGAGGTTGATCCTGGTTTCTTCTTTGCTCACCTGGACCACTACAAGTTCGA GGCTGAGTACGGAACTGGCAGCGGTAATGCTCGTGTGTACATCAGGGAGCCGGTGGCTGGATCTCCCAGCTACTGGACAGGACCTGGCTACATGAAGGTGACGGAAGGAGACAGCATTGAGTTCACCGTCAACAACCTCCCATTCAGCACCTACTATGACATTGTCGTCAGATATGACCCAAGGATGCCTGAGGTTTT

131

Leucine Rich Repeat (LRR)-containing proteins (Leucine-rich repeat motifs have been identified in a large number of functionally unrelated proteins including ribonuclease inhibitor, and toll- like receptors ) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: GGGGAAGAACCACGATTACTGCCAACATAATTCAAAATGGCCGACTGTAAGATCTGCACTCTTGGAGATTCTTCTTCTCTCATTCGAACAAGTTCAAGGTCACGTCCTTTCATTTTTTCTTGCAGAGTCACAATTTCTGGGTCTTTCTCTTCCATGGCCTGTCTGTGTTTCAGTAATTGTTCTGACGTCTGACCGTCCACAGTGGACAGCTTCTCCCTGAGCTCGGCCTGCGTGGAACGTTCCTGCTCCAGTTTGGCCTGAGACTTGTTCAGCTCTACTTTCACTTTGCCCACCTCCGACATGGTGTAGCTATTCTTCTCCGACACCTCCTGAGCCATCTCGTCCATCTGTCTCTTCATGTTCTCCAGCTCCAGCTGTAAGTTGCTGTTCTTGTGCTGTAGCTGCGTCAGCGAGGCTGTCTGCTGGTTGCAGTGGCTCTGGAGCTCGTCCTTGCTCGTTTGCAGCACTCGGATTTTCTCCTCTAATTGGACGTGACGTTGCTCCTCCTCCGACTTCAGTCGGTGCTTGAGAGATGATGTCTGCTACTCAGCATTCAACTTATCAGCCATGTTAGCTGCCTTCAGACGGCTAATCTCTTCCCCCATGTCCAGTCTCTGTGACTCCATTTTTGATATGCGGTCTCGCAGAATACGTTCCATTTCTTCACTCTCTTGTTTCATTCGGTTCATATCCTTCTGTTTGTACTGTTCCAGATCTCGCATGGCTTCCTTTTGTCTCAGCTTCTCCTGGGTAATCCTCTCATCAAACTGTGAGCCTTTGAGCGTCAGCTCAGACTGTAGGTGAGTCACCTGTTCCTTCAGCTCGTAAATTTGTTCTTGCTGGTGACGGCATTTACGTTCCAGGTCTTCAACCTTGTTTTGTAACTGGCCGTTCTTGTCCTGCGCCTCGGAGATTTCCCTCAGATACTTGATTTCTTCATTTGCTCTATCCTCTTTCTCTCGACGAAATTCATTTGAATGATTCACAGCCATTTCTTGCATTTCCGCCTTGAATCGATTGACAGTTGAACTGAACTCATTGACTTTCTGCTCTGATAACGCCAGTTCCGTCTCAGCCATAGATAATTTTGCTGCCATAGAGTTGAATGCACCTTTCCTCTCATCAAGAGCCACTTGCAAGTTCCCAATCTGTTGGGTGGAGACTCTGTGAGATTTCCTCAAGATTTCATCTTTCTGATCAATTGCAGACATTAAGTCATTGATCTGAAGTTTCTTATCAAATTCCAGATCACGGATGTGTTTTGCCATTGTCTGAGTCCTTTTGAGATTTTCATCTACAATGGTTTGTCGATCAGCATTGCCAGAAACTGCAGCATCAATTGATTTTGTGATGTCCGAANGGAACATTGTTTCCAGCCAGC Mannose-binding lectin precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: TGTGTGGCTTTCTGTTTGATTTGTAGAGGTCCCGATTAATTTATAGAAGTCAAAATGGCAGTTCACGATGCCTCGTAGAGCTACCTGGATCATCCTGTGTCAATTTTTGTGGGCTCTTGTTGCAGGAGAATGGAACACCAAGGATTATCAAAGACGAGAACACAGCTTAGTGAAACCGTACCAAGGTTCTGGCATGGGTGTTCCTCAATGGGACTTCCTGGGTAGCACAATGGTGACGAACAACTACATCCGCTTGACTCCGGACAGGCAGAGCAAGATTGGAGCTATTTGGAACAATGTGCCATGCTTTGTACCAAACTGGGAGATTCATATCCAGTTCAAAGTCCATGGCAGTGGGAAGACACTTTTTGGGGATGGCTTAGCTGTCTGGTATACCAAAGAGAGGATGAAAATTCGGAAATGTGTATGGCAATGCAGATCCCTTCACTGGCTTGGCCATTATTATGGACACCTACAGGAAACACAATGGCCCTCACAATCATAAGCAACCATACATTGCTGCTATGTTAAACAATGGCAGGCTCACTTATGACCATGACACCGATGGGACACACACACAGCTGTCAGGATGTG Partitioning-defective 3 homolog (PARD-3) (may be critical for cell migration) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Partial: CTTCTGATAACGGGCAAAGAACANGAAAGCTCAGAACTTCTGACAAACTGTCATCGTTCAGTTCAAACTCTAGTAGTAGTACAAATTCCGGTCAAAAGCTTGAGAGCCTTTCTAAACCAATTCCCAAAAGAACATCATTTGCCTTGAACAACAATGTGATCAAACCGGGCCCCCGATCAAACCACCGAAGAAAACGCCCCCTGCTGTCCCGGTGCGCAGTCCCAACACAGGGCTCACCCCAGGNCCTCCCACTGACTTGCCCTCAACACGGC

http://en.wikipedia.org/wiki/Ribonuclease_inhibitor�

http://en.wikipedia.org/wiki/Toll-like_receptor�

132

TTATAATACTCGCAAAATTGGCAAGAAGCTGGGTATCCAGCTCAAGAAAGGTCCTTTAGGCCTGGGATTTAGTGTGACATCTCGGGACAATCA GACAGATGGGAATACGCCCATTTACATCCGGAATATTCTGCCCAAGGGGGCTGCTGTACAGGATGGACAACTCAAGCCTGGGGACAGACTCTTGGAGGTGAATGGTGTAGAGATGACTGGCAACACTCAGGCTGAGGCCGTTTCCTTCCTTCGCATG Pituitary adenylate cyclase-activating polypeptide precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: TACGCCTCAAAGCAAAAATATAAGGTCCAATAAGAATAATACATGGTGTTTAGAATTAGTGTCTATCATGTAGGGTAAGAACAATAAAAAATCCTGGGTGAGCAGGGAATAATGATATAGCTATAGGGCGGTCCTGGATCAGAGGTATTTGCAGTATCTCATGTCGTACGTTGTATGGGCATTGTGCAGATCTGAAATAAATGTCTTGTGCTTTATCGCACCCAACACAGCTGGGAAAAATAAACAGACAACGGCCGGGGCTGATCTCTTTCCATTCACTTTATCCCACCCACATTAACGAAACGTTGCGAAAGAATAAATGCGTACC Protein deltex-2 (Deltex-2) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: GGATGCCAGCGTGCAGTGCCCGAGTTGTAAGACGATCTACGGAGAGAAGACAGGGATCTGCCCGCCGGGAAATATGCATTGGCGTATCTGTCCTGACCTTCACCTTCCAGGCTACGAGAACTACAGCACGATTATGGTTACGTACAGCATATCTCCTGGTGTACAGGGCCCGGAGCATCCCCAGCCAGGCGCCAGGTATTCCACGAGGGGCTTCCCGCGCAGAGGCTTCATACCCGACTGTGACCAGGGCAGGAAGCTCCTGAAG Protein NPC2 homolog precursor (Niemann Pick) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: CTTACACAATCTGGGCCGGAATGATGGCGCAGAAGACATCATTGTTCGCTCCATCCACCAATTGCCATTTGACAAAAACGTTGACAGAAGGGAAATAGCTTTTGA CTTCAAGAATTGGCGTGTAGTTTTGAGTCGTACTGGCTTTGATGGGGCATTTAAGGCCTGAGTCTTTGCAGGCATCTGCGTTACTGAGAGGAAAGTTCACATAGGGGAAATCTCCAATCTTGCCAGAAACTTTAGATGTTAACTTGGACTCGTCCAAATTGGCTTTAAAGGTGAAATCAATGGCCACTGAAGTGCCCCTTCTTCAGAATAGCCAAAGGTCCCTGGCAGGTTCCGTT Serine Protease Inhibitor 85 Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: TGTCTCCTGACAACTGTCCTCGCGTTTGGACTAGTAGAGGCGAACACACCCGATTACTGTAGACTTGCAGCAGACCCTGGACCTTGCGAGGCCGCTACGACCAGATACTACTACAACGATAAAGAAAAGTCATGCCAGCAATTTGTCTATGGAGGGTGTGACGGTAATGAGAACAACTTCTCTACAGAGTCAGAGTGTCAAGCAGCATGCGTGTGTACTCAAGGATCAAGAAAAATTCGTCGCCTTGGGTGTACAAAACAAGTTTATTTCCACAATGTTTACAGCGGCCAATGCAGGCTATACGGCCAGCGAAAAGGCGGAAAAAACCATAACGGCTGTAATGCAAAAGCTAATCGTTTCGCAAGCGCCGAGGAGTGCAATTCGGTATGTGCGACGTCACACTGCTATCTGAGCCCGAAAAGAGGACCATGCAGAGCCCGTAACGAACGTTTCTTTTACAACGCACTCAGCGGCAAGTGCGAGAG

133

Similar to cat eye syndrome chromosome region, candidate 5 isoform 2 precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: GTCGGCGAGCGCTTCATGCGCACTGCCGACAGCATAATATTGCTTGCAACATCTTTCATCAAGTGTAAGGAATAGTGCTGTCAATGTGAAAAAATTCCACACCCAAAGTGCTCTGTCAAACAAGTGTGAATTTGGATTCTTGTTTGATATTGATGGAGTGATTGTTCGTGGAAAGAAACCTTTGCCTTTGGCAAAACAAGCTTTTCAACTATTAACAAAGGACAGACAATTCAAAGTTCCAACACTTTTCGTCACGAATGCTGGGAATTCTTTACGGAGTAAAAAGGCTCAACAATTGTCGGAATGGTTAGATATTGAGGTGTCAGAAGAGCAAGTTGTGATGTCCCATAGTCCCCTCAAGATGTTTCGGCAGTTTCATGACAAGCATTGTCTGGTCAATGGGCAAGGCCCCATCAAAGAGATTGCTCAGAACATCGGATTCACGCATGTAACCACCACAGAGGAGTTGAATCAACAATTTCCAAATCTCGACAAAATGGATCATCTAAGGA GAAAACCTGCTCCTTGTGCATTTGAAGAATATTTTCCTCGAATTGATGCTGTGATT SN4* (hypothetical protein, unknown function) Gene Bank Accession Number: n/a Reference: n/a Partial Clone: TCGATGCAGTGAGGGATGCTGTGTCCACGAATACTGCGCATACAACAAAATATGCTACCCGAGAATCCATTGCCATCTGGGCTGTCCAGA CGGCCATTGTGTGGGCAAGGGTAAAAACATCTCCAGACGCCAAACAAAAACTGCCCAATTCAGGGAAATCCAGAGCCACCAGCTCCTCCCCAAAGCCACAGCACGGACTCTCGGGCAAGGGGCCTATCATTTCCGAACGTTATTCTTATTCCGACGTGAATATGAAGGGGAACGACAACAGCAGGATAGACGAGGATGGGATATTTGTGGACAGAAAAGACCCGACCACGGTTGATGGGATCTGCATGCCTCTTCCGCCGTGCCGAGAGGGCGTTTTCTGTGGGACTGGGCATAGTTGTACCATGAACTATAACCTTGGATACAGTGTGTGTCAGTATAATCTAGATATCGGGAGCACTCTCTGTTACGATCGTCACGGGAAATTGGCCTTGACCCCCATGACGTGAGGTCAGGATGATAAAAGTATACAAAACGCAAAGAAAAAAAAAACTTTTGTAAATGTGTACGTCGATGAATAGATTATTTTTATATCATAATATCATTTTATTTTGATGTTCAGAGAAATTCAAAAACATAAATGAGCATAAAGTACAATAATTTTGTCTGATATTGATTAAAATGCAGTACACATTTTTAAAAAAAAAAAAAAAACTGAGTTTGGATCCTGCCATTTCATTACCTCTTTCTCCGCACCCGACATAGA Stanniocalcin 2 precursor (STC-2) (has an anti-hypocalcemic action on calcium and phosphate homeostasis) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: TTCTGGCTCTCCCGCGGGAACCCCCACGGCCTTAGCCGAGGGCAGAGCCGTGGAGGAGTCCTGCCTTGCTCTGGCCGACGAGGGAAGCTGTGAGTTTTACACCTGTTTTGAGAACCGCCTGCCCTGTGGCCGAGACTGGTATATGCTGCGACATGGACA GTACTATTGCAACAAGATGCAGAGACTCAGTTCCACCTTCACAGACGCGGGCCAGGCATTCCTCAGTGACGCCCAGCGTTGTTTGACCCAAGCTCTCAAGCCCCTGTACCAGAGGGACAATGTAGACTGTCACTCGCTGGAACACCAGGCTGTAGCCGCCATCACTCCTTGCTTCATTGACAACGCGTTCTGCAGTGTGTTCCGTGACAATACTGACCAGTTTGCCAGGATCTACGATGTGGGCGATCTGTTCACGAGAGGCGCCGGAAAGGTGTGGCGTGAGATTGCGGCCCTAGCCTTGAGATGCACCCACGAGTACATCCGGGACTTCTCCTCTGAGACTCACAGCAACGTCGTGTCCTCAGTCAATGGCATGGTGGACGCCCTCAGGGATGCTGTCGAGGTGAACTAAGTCTCAGCTCAGACTGGTTCATTTCACACTCAACGAACACCGCTACAACCACCATTTTGTTCTATGTTCTGTTGTGATTTATGATCGATAGTCTTAGAACCCCAATATTCATGGCTTCCATTATTTGACCCCCCCCCACCCCCTCCTCCGAAAAAAAGATAAAAATACATTAATTAATTAATAGTCTTGCTATTGAACCTTTAAAATTTTCAAATCTTTGTTCCAAACAAAATTTTCTCGAATACCAGAATCCCAATTCATGACTTCTAGAAAGGGAATTCAAAGAGTGGTGAAAAAATGTAATTCAACTTCGACGTAGACCAATTTACGCCATGGCTCTTTTCGAATTGAGATAACACACACAAAATACACTCTACAAAAGTCAATCCATAATTTGTTTGGAATTCAACTTCTAAGAAAAAAGAATTGAGGGCGTTTTATACTTCCCCGCAA

134

AAAATGATATAATTTTCAAATCAACCATTTCAAGATCTGAACAGATTGACCCTTTTAGATACGGGTACACTTTTTATTCTATAAACCAGCAGATGAGTTTATTTCCCTTGTAATGCCAATACAGGTATACGTTCGTACCTTTCGTCAGTGTTCGTGGTGCTGGAGTTATGTCCCTTTTGTCAAATTAAATGTCTTCAAATACATCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGT Twisted Gastrulation Protein (May be involved in dorsoventral axis formation) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: GCATGTGTTGTAAAATACTGCATCACAATTACTTTTGAAGTGCTGTTTTCATATCAGAAGCCAGTATGGCCAGACCTGAGACACTTTTGCTCCTCTTGCTGGGAACCGGAGCCTGCATCGTTTATGCATGCAACAACGCCATCTGTGGACCACGGGTCAGCAAGTGTATGCTGATCAAGTGTTGTGGCTGTGACATGTCAGACTTGAAGAACTGCACTTGCTGCCGCGACTGCCAGGTCTGCTTGAAGTCCCTCTATACTGAATGCTGTTCTTGTGTTGGTCTCTGTCCGCCTCCAGATCCTGAGGATGACCTTGCAAAAACGAGTTCTGTTGAGGATCTTCCAGATCCTATCCCTGACTTGTTTGATGTCTTGACGGAGGAGGAGGACCATTTCCAGCGATGGAGTACATACACTTTTGCTGTCGACCTGGATAAATTGTTGTTCAAACCGGGCGGCGCCAAGTCCCCGAATGTCAACATGAAGATCATTCAGACTGAAAAAGAGAAGGAACCTCAGCGCAAGTTTGAACCATCAGAAGAAGAGGATGCAATTCGTCTTGGTATTCGCAACTGCACTGTGGCTTTCTTCTCTGAGTGCATGTCCATGGGCAAGTGCAAGGCCTCATGCAGGTCCATGGGAGCTGCCAAGTACCGCTGGTTCCACGAACACGGCTGCTGTCAGTGTGTCGGCAGCTCCTGCTTTGACTATGGACTCAGCAACCCACAATGTCTGAGGTGTCCAGAAACGGCCGGTTTCAAGGAGCGCTA CATGTTTGAACACAAGGAGAATTCCATGTACCACAGCGATGGCTACGATAAAGTGGGAATGTCTCAGGCCTTTGTGGACACGGTATAGATGACAGATACGAAAGTGGGAGAGGTGACCTTGGAGCTCACAGAGAGGTGCATGAACTTGGTGCGTATGCACTCGCCAGGTGTGAGCAGAGATCCAGAATGTGCTGTAGATCTTGTTTCGACGCCTGTCTTGTAAATCTGCTTACTGCAAATGACTATTATTAGATGTGACAATCATCATAAAGTCTGAACACTGATTCTTGACATTTCTCCGTAAAAAATGGGATGATCAATCTTAAACACATTATTAGCATGAAGAGACGAGACACTTTGTACGAAATTACACGGGAATAAAGCATGGTTACTGAGAAAGAAAGAATACCACTAGTTACTAACTGACAAAGTGAAGTTAAAGATCGTCATTTTATGCATAGAGGTTTAGGGCAGTTACTTTACTGATTTCTGAAAGCCAGATGCAGTGAAAC Venom protein 2 precursor Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: CTCGTTATATTTAAAGGAGACTTGAACTACAGAAAACTTGTAGGAGATTTGAAGTGGGATTATACAACCGATTTCCAGACGAGTTTAAGGGGGTTTCTACCAG Von Willebrand fac tor precursor (Important in maintenance of homeostasis) Gene Bank Accession Number: n/a Reference: n/a Comment: Predicted only, not cloned Predicted: CGACGATCGAAAAGCGTAAAGACCGGTGAAGATCCACTGGCCGTCATAAGTCACGATGGTCAAGCTTTGTGCCCAGCCTGGGGCCAGAGTCACTACAGAACCTTTGACAACAAAATCTTCACCTTCCAAGGGACATGCAAATACGTCTTGGCTCACGTGCCCAACTATTTCGAAATCATGACAGTCAACGACAAGATATGTGTGGATGGATTGAACTGCACTAGGGATATTGAGATATACTTCAACGACAACGTTGTCCGATTGAGGTCTGCACAATCTGGGCCCATTGTGGAATGGAATGAGAGAAGGTTCAATATCCCAGGCTCCAGGACAGGCACTGTTATAGAAAGAGTTGGAAGCTACGTGACAGTCAAAAGTCCCTTGCAGTTCACGCTGAAGTGGGACGGTAGTCAGTCCGTGTTTATTACGGTGTCTGACAATCTTAAAGGAAAAGTCAGTGGCTTGTGTGGTCGTTACAATGGAAACCCACGTGATGACTTTGAGAATGCCAGCGGAAAAGTTGTCACCTCAGTTTCCAGCTTTGGAAACAGCTGGAAGAAAACGAAGCTGTCAAATGGCCCTGTATGCTTGGACTTGCAACAAACCAGCGGATGCAGCAACGATCTTGTCGAACAGAGTGCTCAAGCCAAATGTGGAGATCTTCTCACAGATCCCGCTT

135

TCAAACTTTGCCATTCGGTAGTCGACCCACAACCTTATGTAGAGGCCTGCAAAACTGACTGCTGTAGCGCAGACTCTGCTGGGTGTATGTGCGAGACAATGCAAGCATATTCCAGAGCATGTGTGGACCTTGGTGTCAGACTCTCGTGGAGAAATCCAGGGCGTTGCGAAATCCAGTGTGATGGTGGTAAAGTATTCAAAGAATGCGGCAGTTCATGCATCGAAGACTGTTCCAGTGCCAGTACTACCTGCTCTGACCCCACCTGCATCGATGGGTGCTTCTGTCCAGATGGCTATGCATTACACAATGGTGTATGCATACGGAAACAGGAATGTCCATGCACATTGACTGCAGAGCAAGGAACTGGAAAAGAGTATCAACAAGGACACATCATCAAAAAAAAAAGTACCGACTGCG

Found by homology search to Lottia genomic screening

Lottia Protein Model: jgi_Lotgi1_103075_e_gw1.1.1164.1 NR Annotation: PREDICTED: similar to CG31650-PC, isoform C [Apis mellifera] NR E value: 9.00E-50 Ac Annotation: Reticu localbin1 precursor_[1.88764E-10]_[19]_APL_all_052305.1244.C1 Ac E Value: 7.00E-36 Sequence: MNVYYNILILCLGVYCTLGEKEKVPNEPVDQKHTKDGEHNPHYDHEAVIGSKQLEHEFDELPPAEAIQRLENLVKHHDLNADNIITHDELKKW ILASYMSLDEEESDEKFQENDVDGNGNVSW RELVKKEFGYDLEDIEDFKKNTDKQEPDTKEFLLMLDEEEKRFMTADTDNNGNLEKQEFRAYYHPNEYPHMHGVEIERALRTHDKNKDNQVSKAEFIGGLLFLEDREVYLTEEENFNSFDLDGNGKLSQDEVRAW VIQDNNQAAEDEVLHLISQADDNKNNELTLAEILEHYDDFVGSATDYGHKLSDEL* Lottia Protein Model: jgi_Lotgi1_108564_e_gw1.8.40.1 NR Annotation: PREDICTED: hypothetical protein [St rongylocentrotus purpuratus] ref|XP_001179636.1| PREDICTED: hypothetical protein [Strongylocentrotus purpuratus] NR E value: 2.00E-86 Ac Annotation: Kinesin-like motor protein C20orf23 (Sort ing nexin 23)_[3.38462E-18]_[9]_APL_all_052305.1593.C3 Ac E Value: 6.00E-99 Sequence: MYLSCYLPVILLFIEDLKQAVVAMMLRKDEVEEKNKSLKAMLDREMEISSTLRAEIEEMKISFKLSKDKEIAKNETLQKENELLKHQLRKYINAVQLLRTEGAKDDTQGITLEDPQPIIPPAKPSIDYSHEASEYEKKLIQVAEMHGELMEFNELLHRQINCKEAVIRNLKEELTDLRGPLPYDAQSSDDSLSGDLESSLISRCLINIWIPSAFLGGSKTDSHHVYQVYVRIRDEEW NVYRRYSKFLDVHTRLKKVYPLIEKFEFPPKKTIGSKDPKVVTARRKMLQSYLRKVINHLLEKNADLSSNVSKEKLIAVLPFFK* Lottia Protein Model: jg i_Lotgi1_118077_e_gw1.28.72.1 NR Annotation: LOC100124858 protein [Xenopus tropicalis] NR E value: 1.00E-108 Ac Annotation: Tubulointerstitialnephritis antigen (TIN-Ag)_[0.0]_[3]_APL_all_052305.11433.C1 Ac E Value: 8.00E-78 Sequence: MGSTLNILVILGCILVPFSFSNFLEW GPDLEGPW CATRPIEQCCPGRDDECTVPILGTKCYCDIFCNETAEDCCPDFW NLCLGVTRPTPWPLTTTTSRVPINILCNVYW FKCTKLHFSSHWECSNDDCLLEADHITQINNGPYSWVASNYSDFW GLTLEDGVKYRLGTFPLGSNVVQMTPLRVKLTDVLPESFDARTKWPSYIKPIRDQGNCGASWAFSTTAVASDRLAIESLGEIKDELSAQHMLSCNVRKQKGCEGGNLDRAWWFLRKTGVVTEECYPYES GDTTDKGDCLVSRRKSNTTCPSRILYKEKRRYKATPPYRIAPKEREIMKEIMDNGPVQATFMVKSDFFMYKNGVYRYSNISFDEEPAVYHSVRIIGW GVERTIYGDILKYWICANSW GTQW GENGYFRIIRGRDECEIESYIVGVW GQISGDASLRTLLTRGRRRRLFAERSLRNLRVNSMAKGRKSRSRQVGRKKVKRGRKSREERRRLRQEHRNKRKLRRQSRKQDSDSQNTV*

136

Lottia Protein Model: jg i_Lotgi1_134336_e_gw1.89.95.1 NR Annotation: apolipoprotein D [Branchiostoma belcheri tsingtauense] NR E value: 9.00E-41 Ac Annotation: CNSN01-F-078834-501 Ac E Value: 2.00E-25 Sequence: MERILTAVLLCIGVAVYA QVPGLGPCFDAKPQSTLNVSQYLGHW YEITKLPSFFEIEQVCLTANYSVKEDGHIKVFNQGKVNGKNVSSTGDAYVPDPSEPSKLIVKFPQAAPYGPYW VLDTDYTSYSLVFSCEQLGSFAHAKFLWILSRTPSIDPAVKDRLFKRLEKDGIGASGLRATKRSDCS* Lottia Protein Model: jg i_Lotgi1_154590_fgenesh2_pg.C_sca_6000093 NR Annotation: PREDICTED: similar to meteorin, g lial cell differentiation regulator-like [Tribolium castaneum] NR E value: 3.00E-24 Ac Annotation: Meteorinprecursor (RJD6)_[1.49345E-11]_[5]_APL_all_052305.10513.C1 Ac E Value: 5.00E-43 Comments: Secreted, involved in both glial cell differentiat ion and axonal network fo rmation during neurogenesis. Sequence: MDFLQATWLLVVMVIFHIYIITADQICNQCDCTIRDSTGKGNINVKPKCQSGKITWPDPVSAVRLELKPEVVGPFKACIALKSENIVTLVSREITTETVNYRTRSYDSKVDLLPWFSVEGQGKEHCVTSSESSVMLYLEIESTTEDSGLSRTFIYYDIELIPDNKFSPDPMEECQPCSDDEILRSYCISDYVVVGRMKEVNFQSELDKTKVNIIVDQIVKTTDEDIFKRHDNLLEGALFTSVHCGVQKGPGQFLFTGRNRLGQPILTCAPFYDQWIQIQRLAQEKGTIECAYG* Lottia Protein Model: jg i_Lotgi1_160631_fgenesh2_pg.C_sca_25000236 NR Annotation: Substrate-specific endoprotease Tex31 precursor emb|CAD36507.1| substrate-specific endoprotease [Conus textile] NR E value: 4.00E-29 Ac Annotation: Delta-like protein 3 precursor (Drosophila Delta homolog 3)_[5.19686E-34]_[5]_APL_all_052305.2047.C1 Ac E Value: 2.00E-05 Sequence: MNRLCFLLVVW QIALSQSKVVSSVEGFNGNLLRIVRSANNAAPQGNLNYNSNCNEAYKNISSSHSMCLVDVCTAVALSQEEIDAAVAAHNDYRKNVSPKAAAMQKMVW DDELAEIATKWAQQGRAGHDTKDARKTISLKGTYIGQNAAAGQANLIAAIKAWHDEVVDFKYGVGSINGKDVGHYTQVVHHQSTRVGCGKAHCPDSTYKSYYVCNYAIGQMGLTHPYKSGSSSCSDCPSSCSDNLCDCNGKICFNGGTLDINTCTCSCPSLYSGDQCQTLQCTKTEKSWCRRSYTVADCTKYSNFPIDCNMMCGVCPYPCDGKQCENGGTLDPNTCQCTCKDPYTGPTCADKSCPGQEEWWCQKFFTAADCTKYSNFPTDCNIMCGVCPACKITA* Lottia Protein Model: jg i_Lotgi1_163300_fgenesh2_pg.C_sca_39000034 NR Annotation: PREDICTED: similar to TTC17 protein, partial [Bos taurus] NR E value: 1.00E-133 Ac Annotation: FlbAprotein_[0.0128025]_[2]_APL_all_052305.15119.C1 Ac E Value: 2.00E-84 Sequence: MASSVVVIVLLHFLFEQCLLTRGSMHW VVTEDGRIQSQADSVYNLRRPYDLVAFMHQEDRATVLNHLKKELLNRKGEIDKSEDRDTTGLEQKFYKSDYDCLQAGTPLPEIDLYISTILPIEHKHISDELSIEIQKAAQSSDNKIGTIPPEPDCTKVLQLDFTPHGFEHLEGVQERMNLTGSAELGLTHAIPFIPDKTEIPDFGHVIAKELKKNSTSWVLYNMAAFYW RVKGDPWMTVECLRRALHFSPRENKDIALISLGNILHRARYSNEAAIVVHSALELSKELNVNHFTLGNIYAVLGEYNKSIICYENTLRIQPDFEAATIRKHAVLCHQKLEAALEAQHRSLQKTLRDLKDYQKKHDLWHIQNDRMSVEQVSQEEKVSQNIAFEFNKAKQTSTKDIGEYCNMVEREGKQVLLCTWNRQAPTLEMLDQFALEEQKKESERNRLKLNKYEKKAIDYNLPVRAPLYVKHDRQAVPRFNELELDNTWPEKEECDSH

137

LLTSPDPFNLSTVYLSPENKGFEVKALLTEAQNLKDGAEHPLPW YPPICVTLLTINEYDEKTYDNLKSVGHPGRTKVPLKMYDPSMRKTLLSHVNGGTVTEEEVGQRILSAIKQEVGARW VLFNLAGLYW RIVGNNYHGIECIRRSLALAPDEYADVPLVNMANILYKW GRYDDAIVLMKDALKINDAEPDSNFLMANLLW VTKNYSGAISHYRTVLDIIPEHQEAFDSLRAIKCLQKFLIKEQSVAPIEVPSQPVGNSCQQKGVANKNQQTESRVICKTENGEEKCIIETRMRGKSGECNGHCTQTCTITPIKVESSCGGPDLAVDTSNPVQCHQKGSQCGEAGEELLFTNDFTNKLDEVSDHYEKKGICNGEDCNTLRVQCLIPMKTHSGLVAHVITPPKLFVRPLSLHSTQCMTEQKKPHIKLEFIDGVLHQKLIFVQDANDIHVEDNECIIFNDGIKSPGCDQAQYRSYSEELARSNINVEFRYVGDESPVEKDKKNHCSKTPNSPGVPSDEIPYSDISDGILQSLNGDVEPSEVVGHRIALTLQQNPKSW VAASAAGLYW RVEGDFTKALDCYRLSLSYAPLEMADLMLLGYANILVQGGYYSNAIMVEDLALDIQSTLPIHYFTLGNIYAALGKWRTAVSFYEATRFYQEDFKPAVDRLRAIHCENIA* Lottia Protein Model: jgi_Lotgi1_168805_fgenesh2_pg.C_sca_79000073 NR Annotation: PREDICTED: similar to plexin A CG11081-PA, isoform A [Apis mellifera] (a neuronal semaphorin receptor that controls axon guidance) NR E value: 1.00E-121 Ac Annotation: PlexinA3 precursor (Plexin 4) (Transmembrane protein sex)_[1.07485E-23]_[4]_APL_all_052305.11722.C1 Ac E Value: 1.00E-27 Sequence: MEGGIRLQLFLLVSLICHTQCIKILHSFRDPGGNILQKLVIDDQTGNVFLGALNRLHKLDPDLNIVQSASTGPRLDNVECPPPLLPCDKPKTQLNSQIKGLVIDSASNSLILCSSLFHGSCQTLALNNITNVKKFFHKPLVPNDHHSCYMFLAPSINNTQALYIATAYSDLGDAAYRDLVPSISSRSLDNLDFIHRDTEGSTKLEILKEYRESFIVRQLYGFQNGGFVYFITLQRENPGSQKIVSRINRLCQQDRYFRSFVEIPIKCQKSDSDSYDVVQNAYFTNGKLYVIFSNTVSSAGAKKSTFCEYDIQDIDLEFNETVRNCYKAVGNFGPEHYKKIEPCPPLIFGSVDFCGESGEW KDYSAIEGSKPISATPLHFFTSDKPTAIFVHPEGNHRYAYIGTQDGHVIKLRFENGQVNQLFKITHSVGDIILQITNHPQSNLLYILGSHKLSVLSLNHCDDLTSCDECMNDGDETCGW CVMDNRCTTKELCQSSVITPPW LSRTDQSCASISSLQPSSLSYKKFINGGDSRQIKFQVNKLKFESNRNLNCLFINGEKHDNTMAILDQKENTITCPLPRQLPTIPQGKDHEDLELQFHVEGKMIVKRDVSVFDCQGNNNCTSCSTSQFNCHWCPVSHSCVEQSESCPQGDSIKVNDRCPRLETAASDTDILVHSGELKTISVQVRALEANQRSNLKCHFDLSGQVQTVSAKISSSTLTCETIKLAYADDIPYIVAGFKVTW GQQNHPLDNPQRMNVRIYKCASMVTNCGKCLSLDVEYECGWCEDQCSLQKKCQKSWLDQSETCPNPQILRYSPSKGPVKGKTEIQVNGINLGKEISDILGQVTVAGLPCDVMQEHYEPSSSFKCEVKGVTSAKSGTVKVVVNNKYTAESDTVFEFVDPVLNHVDPKQGLMSGGTRVLIKGNNLDVGSDTQVIVGGNSVPVIK* Lottia Protein Model: jg i_Lotgi1_192261_estExt_Genewise1.C_sca_450209 NR Annotation: conserved hypothetical protein [Aedes aegypti] gb|EAT46740.1| conserved hypothetical protein [Aedes aegypti] NR E value: 1.00E-128 Ac Annotation: Ero1-likeprotein precursor (Endoplasmic oxidoreductin 1-like protein)_[2.65914E-25]_[1]_APLCNSN01-F-139925-501 (oxidoreductase activity) Ac E Value: 1.00E-38 Sequence: MTLYITSLVFFLFINGTLQVKNRSSKVADRCFCKLNGEIDDCSCKVETLSVLNNNKIYPRIKSLLSRNYFRYFKVNLKKKCPFWHDDSRCALKDCHVDTCKEEEIPSAIKKGSSAYRYSEEAQKEESCAEEKELSALNTTISDESIQAFKDW KEHDESQDLFCDIDDESSADSEYVDLLLNPERYTGYKGESPHRIW RSIYQENCFKPQTEYIYGPSKSSSPLCLEKRAFHRLISGLHTSINIHLCADYLYQENLGYGMKAHWKPNVAEFQKRFDPQTSNGEGPQRLKNLFFTYLVELRAIAKAAPYLQEEDFFTADSDEDKDVRQGIDDLLNIISTFPDHFDESKLFAGNPNEAKLLKDEFRNHFRNVSRIMDCVGCDKCKLW GKLQTQGMGTALKILFSGDDIGPDSTVNAQQKKHFQLTRSEIVTLFNAFGRLSKSIHSLEMFKSLLSKS* Lottia Protein Model: jgi_Lotgi1_197084_estExt_Genewise1.C_sca_820083 NR Annotation: glucose-regulated protein 94 [Crassostre

138

NR E value: 0 Ac Annotation: Heatshock protein HSP 90-beta (HSP 84) (HSP 90)_[0.0]_[23]_APL_all_052305.1.CB102 Ac E Value: 1.00E-105 Sequence: MKRFIVLGILGFLFIAGAGLLNVQAEEVVTEPDVVEDIGKSREASRTDDEAVQREEEAIKLDGLNVAQMKELREKAEKFAFEAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRFVSLTDKSAMAATDELSIKIKADKASHVIQITDTGIGMTKDDLVKNLGTIAKSGTSDFLSKLGESKDQAQMSDLIGQFGVGFYSAFLVADRIVVTSKHNDDDQYVW ESDSSSFSVAKDPRGNTLGRGTMITLHLKEEAHDYLEESTIRSLVKKYSQFINFDIFLWASKTEEVEEPIEEEEEEKKEEEKKEDEDAEVEEEKEEKPKTKKVSKTIWDW ELINNVKPIWTRKPSEITDDEYNDFYKSISSDYEKPMTKIHFTAEGEVTFKSILYVPNSSPSDTFQNYGKQMEQIKMYVRRVFITDDFEDMMPKYLSFIRGVVDSDDLPLNVSRETLQQHKLLKVIKKKLVRKTLDMIKKIDKDTYEKFW KEFSTNIKLGVIEDTTNRTRLAKLLRFSSSNSDTELTSLAEYLERMKEKQEHIYFVAGHNRAEVEKSPFVERLLKKGYEVLYLTEPVDEYCIQSLPEFESKKFQNVAKEGLKLDDSEKAKERKEATEKEFEPLITWLKDSALNDKIEKATISERLASSPCALVASQYGWSGNMERIMQAQAYAKAKDPSQEFYATQKKTLEINPRHPLIKELKKRVEANKEDETAKDLAVVMFETATVRSGYRITDTAGFAQRVENMLRLSLEVPLDAKIEEEPEEEPEEAEEEKTEEVEAEAEEEAEEKKSEHEEL* Lottia Protein Model: jg i_Lotgi1_204770_estExt_fgenesh2_kg.C_sca_820009 NR Annotation: unnamed protein p roduct [Tetraodon nigroviridis] NR E value: 3.00E-35 Ac Annotation: Translocon-associated protein, delta subunit precursor (TRAP-delta) (Signal seq_[3.38716E-37]_[8]_APL_all_052305.232.C1 (bind calcium to the ER membrane) Ac E Value: 3.00E-46 Sequence: MAATTHKMFLVACLVFLPIFASADTCLGPSVSAETYTTSEVTVSTETVFIAQFTLTCKNGLQNVNLYAEVAGRMIPAVKTSKPNEYQISITDEYKALRSGTYEAKFYDEESFSALKKAKRNNEDSNSVTPLFSINISHSGLWQGSYIQSEFVAACVAILVWW LAFSAKSKLSP* Lottia Protein Model: jg i_Lotgi1_211937_estExt_Genewise1Plus.C_sca_110124 NR Annotation: PREDICTED: similar to SPFH domain family, member 1 [Monodelphis domestica] NR E value: 1.00E-124 Ac Annotation: ProteinC8orf2 (UNQ2441/PRO9924/PRO5003)_[0.0]_[11]_APL_all_052305.7501 .C1 Ac E Value: 1.00E-117 Sequence: MANPALALLSVSAVALAIFLNFSIHKIEEGHVGVYYRGGALLSSTSEPGYHFMLPFLTTFRSVQTTLQTDEVKNVPCGTSGGVIIYFERIEVVNKLDRAIVYETVRNYTADYDKTLIYNKIHHELNQFCSVHNLQEVYIDLFDQIDENLKKALQLDLNVMAPGLMVQAVRVTKPKIPESIRKNYEAMEGEKTKLLIAIQKQKLVEKEAETERKKAVIEAEKVALVAKIQW EQKVMEKESEKRMSEIEDSANTARAKAKADAEFYKTQKEIESNKLKLTKEFLEFTKYESISKNTKIYFGNSIPGIFSDKVEPQEIAKVVGERK* Lottia Protein Model: jg i_Lotgi1_116422_e_gw1.24.410.1 NR Annotation: PREDICTED: similar to CG14764-PA [Apis mellifera] NR E value: 7.00E-58 Ac Annotation: Retin itis pigmentosa 1-like 1 protein_[0.0216154]_[1]_APLPEG003-C-222184-501 Ac E Value: 3.00E-13 Sequence: MKTKNFYILVLLISVTSVALW LVSYPSPTFRNIMSTTQNNIRKIPKHMQMSKSPDLEVDKKYLDLLGFDSESKPKNDIVVIGSALMPADTENTILFLKSAQEYVPNLKLVLFDMGLSKADKQTLTNCRNETKHCEIRKFNFDQYPSHISDFANKSYKPICIQLLLKEFGTVIWADTSELFASSDIDNTMKQAKTEGLVAWTIEDATSSLTHPKMFDYFKTDQKKYFFHHAVQTSHIILVNTDKIHENVMLPW VKCALVEECINPMGAQNSGCNYQRKPKFRYTGCHWYDMSALNVILGLAFDFDEAYSGKDKIFWTKFDQEKAKAENMTDGAVHKVSVPHRVYF*

139

Lottia Protein Model: jgi_Lotgi1_118302_e_gw1.28.365.1 NR Annotation: No match found NR E value: Ac Annotation: HISTIDINE-RICH GLYCOPROTEIN PRECURSOR_[7.36731E-10]_[4]_APL_all_052305.6806.C1 Ac E Value: 1.00E-16 Sequence: CVMLYMLCKCSSLDCVKLYVLCECSICHCVKLYVLCECSICHCVKLYVLCECSICHCVKLYVLCECSICHCVKLYCSICHCVKLYVICECSICHCVKLYMFCECNICHCVKLYVLCEV* Lottia Protein Model: jg i_Lotgi1_149784_fgenesh2_kg.C_sca_15000101 NR Annotation: PREDICTED: similar to chromosome 19 open reading frame 10 [Canis familiaris] NR E value: 9.00E-20 Ac Annotation: Structural maintenance of chromosome 1 (DA-box protein SMC1)_[3.12337]_[10]_APL_all_052305.5506.C1 Ac E Value: 2.00E-18 Sequence: MLISNDFKCLFMLILGFIQW VSSYEMTRDYEEASHFDVMPGGAVLSHEKQLDGFTCKFTYACQGGTKEGWQLTINTNTDKTKYLCSVQRPDGKSYLFFQSFKLEFTGLEVKEGYAMGANFKPLSPSEYQVDFQKHSISDVAGKFGSQLEKVELYGEKGKAEL* Lottia Protein Model: jg i_Lotgi1_152847_fgenesh2_pg.C_sca_2000267 NR Annotation: SARP-19 precursor [Littorina littorea] NR E value: 5.00E-08 Ac Annotation: CNSN01-F-148317-501 Ac E Value: 2.00E-07 Sequence: MIAFICVLGFISGCLAAPCTHTNSAEHTEEQVIARVIALADHNNDGILSVTDLAAYFVINFDHNHNSEVDKNEFILQWHETFHDEKAFAEHVFTHFDTNADNVLNLSDLFALNIRLDTDGNGTVTNKELESYLQLIYNAC* Lottia Protein Model: jg i_Lotgi1_158797_fgenesh2_pg.C_sca_18000147 NR Annotation: predicted protein [Monosiga brevicollis MX1] NR E value: 5.00E-92 Ac Annotation: KILLERTOXIN KHR PRECURSOR (KILLER OF HEAT RESISTANT)_[0.162197]_[4]_APL_all_052305.10913.C1 Ac E Value: 1.00E-15 Sequence: MKIHILAVLFSFFSATNAATLRFVSQTPLSITINKDGSYTVNVNGEPW LESSAIYFYAEGRRVSMGDGSLKIASTFMDNGYDSVGQYGKTCW RFEIESKYIDTCFKQYMEPTPFIIFEQHYLNDTENTASIDYDYTIAGFPAFTAPPTGKLGYLHYAGSMTGDSDKSFGIWQEKAKMLTGVAGGPLVLFDKDADDCLILSPLNQFMAASSLFDGQNYSTGVLGGVNFVPKDFVLQTVLFYGKGINHAMSDW GLLLTQYYGKDDSYRKSDLTLNYIGYW TDGGAYYYYNTEPNKTYQQTILDVRQNAKDIGMPYKFVQYDSFFYYKGPHGGVKTW VPMPDLFPDGFQYLYNQTGW PAGAHNRWWSPKTTYARQNGGNFDFIVEEAKAVPTDQAFWDYLFDSSKKW GLTLYEQDW LDNEFAGVKALLTQIDLGRTW LMQMGRAAQENGITIQYCMSNPRHIMQSVEIPVVTQARASGDYRAGKDNW KIGISSIMVDALGVAPFKDNFWSSPDQPGNPAYPDLKETHADLQVAVSTLSTAPVAPGDMLNKTNVPLVMQCCNADGMLLQPSKPAKAIDAQILQLAFGGDVGPQGDIWTTFSNIAGFYFGIILGTDMTADYNLTPSKASFKAFLPSEVFPRFASIPKSQEFSASNPLVITTNCTKTNICLYYTSPVFSPGGTNVLIYGEENKIVPMSPKRVTDITLDRDLKLTLTGAAKESVRFGFILPDQYITITCQLGQSGSAVLSFNERSCNGY*

140

Lottia Protein Model: jg i_Lotgi1_159188_fgenesh2_pg.C_sca_19000225 NR Annotation: predicted protein [ Nematostella vectensis] gb|EDO44886.1| predicted protein [Nematostella vectensis] NR E value: 2e-07 Ac Annotation: Low-density lipoprotein receptor-related protein 1B precursor (Low-density lipo_[3.7488E-24]_[1]_APLCNSN01-F-143892-501 Ac E Value: 2.00E-06 Sequence: MRGPTWSVILLLINLIVTLQACDDVTEFQCADNNQCISKTDTCNTINDCDDGSDENNCACLEKLGMEDGTILDSSLIASSSKTGSSPSYARASNDKGNW CPKSDDSKPFFKVKFDTPRAISAIVFTLKQSVVDPLNYLSTWTLKAVLPGSDEEVTLYEGLNAFSNNSLYRSLFFPSLVTDTFIIAPTTW QNEPCLSFELYGCNIDNLCPTGCQNNGKCSGNDICACADGYFGTKCQFQKTTIPFSSYIFKSLESGSLVTSSETFTVRTSTTIRTSKIIEIQGRKVLSLPVADYFTAPKDGKFGCLSKLGSGGCKSFYLALGCELEELKDTVQLFTTGSDSTYIGAEATLKSDNLVVTFRTSELIWTLNVRKTNMFSSSIKYVLEFDWDISTGIRIYVNKKLIGQQRQATRTTQVLRTNTLATSGVEFFKSKSVNVKLFSLKSSSVNRISLIKTSLISLTPTEECDQEKEHKCANSDKCIDKSNVCDSVEDCDDGSDEQNCPTTTCDVKVYKKLVTKSASLSLDLLEVDDAQRVAKCTLPVISYVVDGILLDFLSKNQYGDQLSATNDLGANQNHNNNNKDDNNNNKKSSQDEFKEDVFNWADEPWTPCDDVEVPDWARMDDPTFDNFNSELIWAEDPWTQLPLVGLPSWMLLPGIDYPVEAKDPVVYTPVNDEDDAAFLASGIDIVNWSDEPYFQLPLDVPRWALLPGIDYPVRHGYNSPYWTYKLNQYNSSRREFGKKMFARQFPTLREERVLYNNNGQSKLCIPGNFQRRNHRF* Lottia Protein Model: jg i_Lotgi1_153437_fgenesh2_pg.C_sca_3000340 NR Annotation: hypothetical protein PB105991.00.0 [Plas modium berghei strain ANKA] emb|CAI04701.1| conserved hypothetical protein [Plas modium berghei] NR E value: 8.00E-09 Ac Annotation: DAZ-associated protein 1 (Deleted in azoospermia-associated protein 1) (Pro line_[0.0]_[6]_APL_all_052305.517.C1 Ac E Value: 3.00E-09 Sequence: MKTMVAIIGVIIVSCVLHTSGLPLFKNILNERSVEYGGESGNGFKNERAVGYGGESGNGFLNERSVEYGGESGNGFQNERAVGYGGESGNGFLNERSVEYGGESGNGFQNERAVEYGGESGNGFLNERSVEYGGESGNGFQNERAVGYGGESGNGFLNERSVEYGGESGNSFLNKRAVEYGGESGNGFLNERAIEYGGESGNGFLNERAVEYGGESGNGFLNERAIEYGGESGNGFQNKRAVEYGGESGNGFLNERAVEYGGESGNGFLNERSVQNSGKSGNGFLNERSVQQGGKSGNGFLNERSVEYGGESGNSFLNKRAVEYGGESGNGFLNERAVEYGGESGNGFLNERSVQYGGESGNGFLNERSVQYGGESGNGFLNERSVEYGGESGNGFLNERSVEYGGESGNGFLNERSVEYGGEAGNGLK* Lottia Protein Model: jg i_Lotgi1_160696_fgenesh2_pg.C_sca_25000301 NR Annotation: No match found NR E value: Ac Annotation: Translocated actin-recruiting phosphoprotein (Tarp protein)_[0.451769]_[276]_L7ALL-contig08326_921 Ac E Value: 8.00E-06 Sequence: MKLLLLLTVLIPLCVSVPTKPGCCVPPVW EGIQGMFSGDGSGPAPELIQQSALISYDEKRQMININITQSIHGLTSDVRILTDYKKGVQYLINSKSRSCTTSKVPQTFQRFCIPDYFYYKGSFNVAFTLRASTEQFIVPLIWSKCKSNSSNFFKMRKACFSRDIQLVVGTCDCLFN* Lottia Protein Model: jg i_Lotgi1_163475_fgenesh2_pg.C_sca_40000029 NR Annotation: Myc homolog [Crassostrea virginica] NR E value: 8.00E-08 Ac Annotation: 104120_3728_3444 (Unknown)

141

Ac E Value: 1.00E-11 Sequence: MDLSYLYYGMFWFSCLLVSTVSAEFSYEEVTYRIPPTPTGFYDQNCTDKISDCEEFGIASCFKPYESWARENCAKTCEFCQGKPEKPPTCTDVEKGCDLYTTSICTDPSFISWAKAKCRRFCRMCPEQVLAELDLMTTTLTPVDCFDKVVCERYGKKACTGDFASWATDNCQKYCGFCSTGVTKSKRLCVDKLPNCPQYDSRICTDKKYYSWTDENCRSTCKFCQDKLVPFLIPTPQAVRTPKPPTT* Lottia Protein Model: jg i_Lotgi1_166720_fgenesh2_pg.C_sca_60000088 NR Annotation: No match found NR E value: Ac Annotation: ProteinC 20orf129_[0.888824]_[1]_APLPEG002-C-006682-301 Ac E Value: 8.00E-23 Sequence: MMLLIKTGVLLIILFSLNTLCDSCACEFHPVLSGKWFFTGENKNINIKIHSKIIVFKVSNYLKLRYKCTERYGNIYLLRILCLFLEVDFLSDLFMKGGFTSIHVEGRGFDSFIFSPKNVTRKYANIDNTCDSEISRERKFIYRKGGCRVPRDMVGTW GFTFRQSSAITMGKRNLTIHFLNNDNLTLNCEARDRNMFLFRRSSYINSSTDAIICVQIDPVLDDPFYDYEFNRIITGNAVENLVRTVPRGKKLYINSDCDMIHSPARPEYIYTVDNIL* Lottia Protein Model: jg i_Lotgi1_166891_fgenesh2_pg.C_sca_62000015 NR Annotation: PREDICTED: similar to scavenger receptor class F, member 1 isoform 1 precursor [Canis familiaris] NR E value: 9.00E-05 Ac Annotation: Mult iple EGF-like-domain protein 3_[0.0]_[1]_APLCNSN01-F-105232-501 Ac E Value: 3.00E-13 Sequence: MATGLYFILSIVTCIINNCVIGLEICEDHYVSCPSQDCGIFPLEPLFRLHCMKTCGVCTDVNRASRKPTAQSSVDVNGMASHAVDGNTDLNICSKTNSSQPDTQWWCSDLQQVYEIDAIKIYSPVIGTKCNTGYYSQSSTSCSPCDTCSENSCEPTNGMCWDGCKQGFKGETCRQCEDGLFGPTCRFACNTTKCQSNCDRLTGICSECDTGYYGKNCELVCDKDCREGKCLKTDGSCTTDGKGSVVSTERAPVQHYTENINKYLVGVIGTFIGIVVTILLVLTYRCFKKRRSDTIYELPTRPPSKQELDFPLDPQYISSGVSTTSSRYTEVHPDSGSSHGSHYLARRNLSQYTHKLRPPAELPENEKRRLQAEMRQSKISGPRGKYMEYPNDAYDRDGSDYEAVKTEI* Lottia Protein Model: jgi_Lotgi1_171718_fgenesh2_pg.C_sca_119000006 NR Annotation: PREDICTED: similar to orphan G protein-coupled receptor HG38 [Gallus gallus] NR E value: 1.00E-15 Ac Annotation: Coiled-co ildomain-containing protein 22_[0.568464]_[1]_L7ALL-ETMYUMG01BM4OM_257 Ac E Value: 1.00E-05 Sequence: MAPIYLTFIASLLLASSTFCEQLCLEGCKCEDHPSEKLGPHLMIDCSNLGSSSVPRDLNKFQNYGRPMHLKLSKNIIARVSKADFPENLPIIALDFNQNRGVMITDDAFMNIKGLRYLGLNDISMPFTDTMKYLKGMTKLRYLYLNKNYQFGEGLVPVSLFSGLELKLKDLSLHDCYLKGIENGALANVVITGSLDIGANQLDHIPEEVKTLKNLTKLDVSQNSIKSIPSNTFEGMSKLRTLSLSNNYINGQSFKTDSFNGLKLKELRLEKSELNTIPSAALSSLSSLKILKLNNSPIKTIPKHSFNGSYCLEVLDLTNVLVEIENTHLDGLESCLQKLYLNNMQIREIPKFFKDFKVLNYVSLSGNSISEIKMDDLVGTTITELDLTENPLHRIEKGSFEEFPHAISLIISDTRLSNLSFVRDYEYGTIKTIKLGNVLPMCDCELAFAEYRVLEIHGNCNFNNTKNVAISTDEFMSVTSHCWPDGKMPQKKDVWAVVGDNSAGTFSTSILSYFCVFLFFKVLNIV* Lottia Protein Model: jgi_Lotgi1_172278_fgenesh2_pg.C_sca_130000017 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO35011.1| p redicted protein [Nematostella vectensis] NR E value: 2.00E-26

142

Ac Annotation: Cellwall protein DAN4 precursor_[8.10371E-4]_[9]_APL_all_052305.453.C4 Ac E Value: 5.00E-19 Sequence: MGVKLLFLGICLIYLVLDLHDAEVTAVSDRQAKLKEKDAREKLQQELSKNYAVKQRRRKKKKFRDPRTRGKSSKVVNRGGAPKVQNGDDGGDDPHIGNRENYLQKYFKGKMW GIRDKKKAPKGNW RKKLRKQKHKKKEKRQNMKKKEKSKRTKVKKFKKKRKKHKKRRRGKRSTDENEDDPGSDQEHTHRTKRYAITDQERMW DYGVVPYSFSSDVTDVMARTYRGVFDALEKRTCISFVEW DGTESFYSNRSLNHMVHLNFVDQGGCWAYLGRYTYRTTNIAQDISCCYVGRTCLHEIMHSLSMEHEHTSPLRDGW LRVNW DNLNEDGQFQFSVIDPLMLGRESW GFDIKSFVSYGTTEFTKNGMMGFQPLMPDFYPTGNSYW QPFKEVQMNYDCFGIKCSGMEEPVCENDGYPGYYEGECGCVCPPGLDPATNCASIKPTDPIRGW PPSSFGLPEPIEGCPAGFQLGSLTQPLAGDSEVSSSSHINAGFENSTAEFGFCLKDTASQTEGGLTEWPRGQYCIHSYSNACPPGFQEGLITYDDSVNATRTILTESPLPDGSFVNNTELW YCCRSDGMVQLDIKLPNTESFILYRHGTAECQNVEGMYMFPEYYSLMIEGETIRSISVYQTLGSVPYNVLDGNELFVYYCYYSPLNKDCGEVIHLGPENPTHDIMTPNYPFDYPLNTECNW LFVAPEGSEVMLNFNDFDVEGDDFDCFDYVTIKRGLPGIPGVSYCGTEFKAAIKSIDRYLMITFTSGSEITYTGFHATGRLLDVADLCYNPDDFGRTYNGIHNYAEDFTECLPW VEMAHCDTNLFNTEDAFDNLDGNYCRNPGARYDSPWCYTEKENCTIKYCDVCQERTIYDNFDDCEELIAEHPNFCLDGDHHHYGCLATCIEYGHITIPEKSNRAQDVSCPVPDDIVDADPIVHDVTRYNVGDSLAYVCSNGTATKMRRCLTDGTWTDIEYVCGGCGDEMKSDTRGTCYAYVAEEVRFVEAEEYCIEKDWDVASAQTEDAFNFLVQYRSDMGSDGKQVW LDLYEDPVGVW SW GDESLVQDGFTNWRSTYPVSGPIETDGNNW YRCATMVGTGQSSVWNQVACDNKW YKAHYICQTMESEREICADRKSSCTRNLIDIPLLCSQYQSYAHEVCPYTCGICDVENADSCTVTADFVVGLNLVSGGSTPFSLNPGESFTTQCPAGQICTQNCQHFSRACRRSGEVTGATPVCQDADSVPTLVNNIERIPRLDTSEARRIYQGDNSYMNITRSGEITKWMTLCENDGIVHLMAFKYW SQDRYFKVMGVNQVECKAGRRMTW EIPEGERIQVDPSMNIGLMDWNGGCIPLYECFASEYPDLNKIYHTMAESQDEVALNKLVWMRAGPTCYFFSLNAEISPVGYIAPATTQLTTPSTTTEEPEPEPEATSTSSEDTSESTDMTTSSGDVSTSSTSTSAGNTESSSTGASRSSPSTDAFVSEDTTSSVW QRTTTHSVVITTPRRTINEDFIKSFIKKKKKKRKWWKNRGKKTDKTRKRKNKRRKKAQKGR* Lottia Protein Model: jgi_Lotgi1_172798_fgenesh2_pg.C_sca_142000011 NR Annotation: gb|AAA30047.1| calcium-b inding protein [St rongylocentrotus purpuratus] NR E value: 7.00E-07 Ac Annotation: P protein [Includes: DNA-directed DNA polymerase ; RNA-directed DNA polymerase _[0.795798]_[34]_APL_all_052305.384.C8 Ac E Value: 3.00E-24 Sequence: MRADSIQVRGLKALVLILLVLYEGHTKETCRTGVKTDKNLRGVNLDKTSGLPFYW FDLHRASDGYHSEYTYTFRLHSSSEASPFSDAVLQVETANNCGTGDLVVNTTFLYQPRNCPKMIMTEYKKPMTNVPFKWRAPKCGCIKFRATVIRNGVTYFYDDENISDGNLVKTVCPHGVRVAEQLVEVDEKTTDSTTKTLSTVTSKMDRAA LLDLLCKITSTLNNLELLKRPEFIQRRKLQNSSLVKLLNIELALEQRKADISSCCHKKGEDKTICFDNVRRYRIDQLCGDGQPRIPFVEQKRNYMRKRESECCW RIGQPRYQCFSKSSEQLTEYSVDYSLDESDPANDIADYPKEVERYEMASNTKFFSPPPTLKPDNQNIETLTKLEETTTPTSVTKSVTLPPIRQPSARPHRQTTPTSVLTHHRQRPVPAVKTKLRQSRTVGQGDMGYNLRKQAKYTRKTTNLRHSASSVEITVNTLKQKLWKSSLKLECCQQGRIFAAKTGRTDVW DRCRSAAKDFRSSVVRGKKICSNTFQKCCVEQFITATAKASTIPKPNTPKGSPSTKQLVTTTSLRTNIKKENYRRAPLDVDSSREADEKYASMESKAEDDLMEQDDKASAEDAGEHVDDDGDDVSDDSDNDDQGEDGDQDEDDDQDDDDKDGDVDDDKVDEINNDEKNRKRKFIDYPREDPSADTFHNLQTKRKSTPNTDIAHRDSNHRNLKIESAKRKRNRRRHNRKLRRRKHKSD* Lottia Protein Model: jg i_Lotgi1_201804_estExt_Genewise1.C_sca_1950010 NR Annotation: DICTED: similar to putative porin precursor [St rongylocentrotus purpuratus] ref|XP_001192936.1| PREDICTED: similar to putative porin precursor [Strongylocentrotus purpuratus] NR E value: 8.00E-19 Ac Annotation: WAP four-disulfide core domain p rotein 2 precursor (Epid idymal secretory protei_[1.82442E-12]_[6]_APL_all_052305.6277.C1 Ac E Value: 3.00E-12

143

Sequence: MRLLIFTLSLLVAVVRSVDASQCPEPPSGFGPCVITDANCLTDSECSNGQICCPHLCGKICKDKVSLTVVKPGTCPSNAAISCFVGSVADTCSSDSDCPGNQKCCNLCGKNCSPPQGSAPQLPPIGLPAPPPVCPPVCAIACEYGNVLDQNGCPTCSCKKTPCKGEAPLTLDRNGNTINCGRGGVRCPLNSECKIHPADAYALCCPIEPDDPKPGVCPKLPNPLPRCLIFQNDCKQDSECPGDQKCCSVTCGIGCIDPVKLCPVVDCLQYCEFGYRLDSNGCQTCQCKKSPCEDEAPMTLDSTGNTINCGRGGVRCPSNSECKIHPADRYAVCCPKVCPPVCEIYCEFGNVLDENGCKTCKCKKSPCEDGAPMTLDSNGNTINCGRGGVRCPLNSECKIHPADRYAVCCPKVCPPVCAIYCQYGNVLDENGCATCTCKKTPCKGEAPLTLDRNGNTINCGRGGVRCPLNSDCKIHPADAYAVCCPKEPSPVCKFGSCPVPPSSFLLRCASIDRCKYDSECEGTKKCCKHPCGRQCRDIVKSKPGVCPKKADPTLVLCKRRGDDCYEDATCPGSQKCCQGACGNACMDPRLTSTDHAGTCPALDPKIQSLILCASLDTECELDSDCKVDQKCCKDYCGIKCVKSVPKPIIKKGTCPRLRYRCRRKRRDFCRSDGDCPGDRKCCRLPCGYKCRRPKRIPIIRKECPAVNTHPIYKLFCSPPLNPPCQVDRDCPQGKICCPGVCANRCLKAPTKPVCDPNPCGDAQIKCQYGQTKDENGCLLCQCKSYDFKMS* Lottia Protein Model: jgi_Lotgi1_205030_estExt_fgenesh2_kg.C_sca_1120002 NR Annotation: PREDICTED: hypothetical protein [Strongylocentrotus purpuratus] ref|XP_001188672.1| PREDICTED: hypothetical protein [Strongylocentrotus purpuratus] NR E value: 3.00E-20 Ac Annotation: Sec sixty-one protein homolog_[0.842179]_[2]_APL_all_052305.13357.C1 Ac E Value: 4.00E-07 Sequence: MKLMFLGFIGILMLSTSNNASASPWFCKGLECPKFNLLNKTKDYEVRQYGSLTW VTTSHIGQWKSDILGELLFKNLFSYIEGNNTMKKQLDLAVPLLTKLESAGDRGSIYTMHLLIPRENIDHLPSPLNTNLTFITIPPSTYYIRSFDGVAYDRQYMKVLQELVNAINDTGAYPDDFFYTATYDHPQVKENRHNEVW LLKKEKIP* Unknown Function

Lottia Protein Model: jgi_Lotgi1_105864_e_gw1.4.718.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO49128.1| p redicted protein [Nematostella vectensis] NR E value: 3.00E-15 Ac Annotation: CNSN01-F-034625-501 Ac E Value: 3.00E-09 Sequence: ILTCLAFSLVWHSHLSGILTCLAFSLAWHSHLPGILTCLAFSLAWHSRLPGILTCLAFSLAWHSHLPGTLACLAFSLVWHSRLPGTLACLAFSLDWHSHLPGILTCLAFSLDWHSHLTGILSCLAFSLDWHSHLTGILTCLAFSLVW HSHLTGILT* Lottia Protein Model: jgi_Lotgi1_107224_e_gw1.6.194.1 NR Annotation: No match found NR E value: Ac Annotation: PEG002-C-001338-501 Ac E Value: 2.00E-06 Sequence: CFLSVCLCSSICFTTECCFLSACLRVCFTTECCFLSVCLLFKRSRVCFTTECCFLSPCLRVCFTTECCFLSVCLLFKRSRVCFTTECCFLSVCL* Lottia Protein Model: jg i_Lotgi1_122612_e_gw1.41.141.1

144

NR Annotation: predicted protein [Nematostella vectensis] gb|EDO49127.1| p redicted protein [Nematostella vectensis] NR E value: 1.00E-28 Ac Annotation: 307649_3461_0681 (unknown) Ac E Value: 3.00E-08 Sequence: MSYRRCFGISCSSMSFAVDWCILLVDGVAW CMLLVDVVDW CILLVDGVAW CMLLVDGVEW CILLVDVVDWCILLVDGVAW CMLLVDVVDW CILLVDGVAW CMLLVDGVEW CILMVDVVEW CILLVDVEDW CILLVDGVEW CILLVDGVEWCILMVDGVAW CMLLVDGVEW CILMVDVVEWSILLVDVEDW CILLVDGVEW CILLVDVVDW LILFGISRFKLELLHLEI* Lottia Protein Model: jg i_Lotgi1_132441_e_gw1.79.137.1 NR Annotation: PREDICTED: similar to CG11081-PA [Nasonia vitripennis] NR E value: 4.00E-72 Ac Annotation: CNSN01-F-064139-501 (unknown) Ac E Value: 5.00E-42 Sequence: MAWKIYVLFVCVLCLRFILAARENYIKATFEGAKDRGIFNKLVVNRETNELYLAAVNHLYKLSPNLEVIQEVVTGPHLDDPSCTSLTNLTCDPDLKSCACKLRPYYTKALVVDYVNKKLITCSTLYHGHCDKLDLDNITNKQMFPFMPMVANNATASTVMFIAPGPAVVKDEPQRQVLYVAASYTRTGLKAYREQVPAFCSRSLEDFNLLLKNAFGSSSIEIESQHRDTFPVRYIYGFGSDNFSYVLSIQKANVQTDRYVTKISRICQSDDDYYSYAEVGLSCTMQNTEFNLLQAAYVGKSGTKLARSLGIPTTEDVLYTVFSIGDSNSANPSATSAMCVYSLRDIRKKFTENIQECFSGIGNTGPDHMIQSSKCLKVVSSHFFVIFFLESFESADLSVL* Lottia Protein Model: jg i_Lotgi1_143169_e_gw1.194.72.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO36917.1| p redicted protein [Nematostella vectensis] NR E value: 1.00E-28 Ac Annotation: CNSN01-F-096652-501 (unknown) Ac E Value: 1.00E-12 Sequence: MVKVCSIPLTVVKVCSIPLTMVKVSSIPLTISKVCSIPLTMVKVSSIPLTMVKVCSIPLTMVKVCSIPLTIVKVSSIPLTIVKVCSIPLTMVKVSSIPLTIIKVCSIPLTVVKVCSIPLTVVKVCSIPLTMVKVSSIPLTMVKVCSIPLTVVKVSSIPLTMVKVCSIPLTVVKVCS Lottia Protein Model: jg i_Lotgi1_145599_e_gw1.360.11.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO49883.1| p redicted protein [Nematostella vectensis] NR E value: 6.00E-16 Ac Annotation: CNSN01-F-006444-501 (unknown) Ac E Value: 4.00E-10 Sequence: LVVYCHCGLVVYCHW ELVVYCHW GLVVYCHW DLEVYCIVYCYW GLEVYCHW GLEVQCYW CLEVYCHCDQVVYCSW ELVVYCHW GLVVYCYW GLEVYCHW GLEVQCYW CLEVYCHCDQVVYCSW ELVVYCHWGLVVYCHW GLVVY* Lottia Protein Model: jg i_Lotgi1_151060_fgenesh2_kg.C_sca_80000004 NR Annotation: LOC431836 protein [Xenopus laevis] NR E value: 1.00E-140 Ac Annotation: CNSN01-F-041871-501 (unknown)

145

Ac E Value: 7.00E-24 Sequence: MDFARLFQSLCICLLTLDIQLFGLTSGANPEVEQHLEMGKKLLAAGQLADALSHYHAAVEGDPDNYMTYFKRATVFLALGKSRSALPDLAKVVELRPDFTAARIQRGSVLLKQGKYKEAKNDFETVLKTSPNNEEAKRNIDKIETIPPDIELAYRLVEEGNYGDAIQVLGGIIDTCPWDPTFHEIRSECYEAQGDLFKAASDIRQSTKLRADNTRGYFKLSSLHYEMGEADESLVQIRECLKLDPDNKQCFPHYKKVKKLVKLMTSAQESINNEEYDKCVKKAEEMLKVESLVHHYVSRAGQHKCKCYSKGGKVKEALAECTHLLKIDPNNLDALIDRAEAHIFNEDYEAAINDYQEASNIDGENRRVKEGLNKANKLLKQSKKRDYYKILGVKRNAKKKAIMKAYRKLAVLW HPDKFTTDEEKEKAQKKFLDIAAAKEVLTDPEKRAQFDNGEDPLDPEEQSGHGGNPFQQGFNPFGQGFSGGSFKFHFN* Lottia Protein Model: jg i_Lotgi1_157590_fgenesh2_pg.C_sca_14000213 NR Annotation: No match found NR E value: Ac Annotation: CNSN01-C-005830-501 (unknown) Ac E Value: 5.00E-11 Sequence: MLMFTSLLFVCFASIYAGESVYPDCQNPDNNERIPNGSKFKLENSGDCIEFTCKDGGYSASSVECDNNGECVAVGRTVEENCRSKKCVYDKFVGFQVTETKCEDDEGQCHSPGEEFTRTYQGTTYSKTTCTVDGNLVKYSYKN* Lottia Protein Model: jg i_Lotgi1_161950_fgenesh2_pg.C_sca_31000192 NR Annotation: RE49668p, putative [Brugia malayi] NR E value: 3.00E-08 Ac Annotation: CNSN01-F-103859-501 (unknown) Ac E Value: 9.00E-12 Sequence: MKLGGDYGILCVLFTILTPVISIDCYACTSLGGNNEGCEDSFERTLKTVHFIARDCYYGYFRGTHCYKLKGERADGSSIMVRHCSDSHW GSHCGDIKYTNEHNVEEKIKGCLETCDHDGCNTATQKQFNFSLVIMAVLMTSALLL* Lottia Protein Model: jg i_Lotgi1_165259_fgenesh2_pg.C_sca_50000100 NR Annotation: hypothetical protein CHLREDRAFT_144736 [Chlamydomonas reinhardtii] gb|EDP05543.1| predicted protein [Chlamydomonas reinhardtii] NR E value: 6.00E-23 Ac Annotation: 220720_2775_1358 (Unknown) Ac E Value: 6.00E-10 Sequence: MKVLLVLDICCLIGLVSVGEAVCPDNCNYNGVCDVKHSRCNCYEGFTGSDCKTDCRCNGHGTCQSGSVCKCDEGWKYSGGQCVW DCHCLNGAKCIGPGECGCVHNCKMGNCRNGQCQCW NGYKGSDCSEYDPTM* Lottia Protein Model: jg i_Lotgi1_166645_fgenesh2_pg.C_sca_60000013 NR Annotation: No match found NR E value: Ac Annotation: CNS003-F-114184-501 (unknown) Ac E Value: 9.00E-13 Sequence: MGASILLGLTILLSYVNQVHLQMFHPRDISRGGQQQEEQLRRGYLIQILKSFLNRRQEDATEKRKRSCNLNLGFHCQTDEYSSIADMYDFLQSALSPGKRKRNVKIVSIEGS*

146

Lottia Protein Model: jg i_Lotgi1_167022_fgenesh2_pg.C_sca_63000026 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO43826.1| p redicted protein [Nematostella vectensis] NR E value: 2.00E-08 Ac Annotation: CNSN01-F-102728-501 (unknown) Ac E Value: 7.00E-34 Sequence: MVAVRIRKYKNLLFFALLFAGVLSISTIFYSNHYQIMNTGTRSDVNTGFSYSNANSSKYVIYICDGKNSCGGYGDRQKGIVASYIISVMMNRQFGVIMNDSCDIKQMFKPNQINWIVDNNDIKSKTSKRIKALDGYSDKLRKSMIEMDLDQEYKEEVIYFSLNLEFVFYLRQNKRYEKQLKW LEHLSMSEIYNVVWQSIFKLRPYIREKLDPFLDLKKQGLKLISAQIRLGKNPTIPHDSRVVNSLNNMNVLW QFYKKYNDSSKYRIFISTDSDTVREKARSIFPDVYSDVPGKVFHVERSNKTDICNGW RKVILDQVILSLSDVLVISNSGFGRIAAFFRQNENDLYCLNLDKIRRCTTQSEIFVDKTW* Lottia Protein Model: jg i_Lotgi1_168040_fgenesh2_pg.C_sca_71000102 NR Annotation: No match found NR E value: Ac Annotation: PEG003-C-229928-501 (unknown) Ac E Value: 1.00E-17 Sequence: MVGTRYLQLATSLAVFLLVLFLTCTQAAPVDDFETDNEALRKALFIARLLSSSDRLKNTKPEGSNLDFSELASIPFSNQQKRYRPPMQGRSGGMSLCLWKVCPAAPW LVSKRSEKTWDKNNMLGK* Lottia Protein Model: jg i_Lotgi1_170051_fgenesh2_pg.C_sca_92000069 NR Annotation: hypothetical protein TTHERM_00439050 [Tetrahymena thermophila SB210] gb|EAR97557.1| hypothetical protein TTHERM_00439050 [Tetrahymena thermophila SB210] NR E value: 3.00E-08 Ac Annotation: PEG001-C-000321-501 (unknown) Ac E Value: 2.00E-18 Sequence: MKMINIFIVVYCLISPCLGFW LSSKTVDPKVDISTECINKALTGDCGFFTCFEERLPCGEYGYAESYGGKYCWQFQQSHHLFTKKGVEFVEKLTRCHMNRSITSYRQNQIECFSHYDQSFVIMGDCYVESGFCDVVIDNFMSLARILEPKDFTNYRVVREVFRAAKQCPNNISGGIISKFLSYKRGSSL* Lottia Protein Model: jgi_Lotgi1_171167_fgenesh2_pg.C_sca_110000033 NR Annotation: No match found NR E value: Ac Annotation: CNSN01-C-005636-501 (unknown) Ac E Value: 2.00E-23 Sequence: MAISHDWSFTLLW LIWIFTLLFSDSYGKRTQNYYMEGLDCGDSKHIGGATVYSNFRGDVSTVYGNDIECQMTFKAENKDWRLMLRIIELDIPDRTSTGLCNDALYVYDESSIYARAMEEANGNTGLCGNILPPTLYSTGQYLTVHFSVSLQNQKDIV* Lottia Protein Model: jgi_Lotgi1_171450_fgenesh2_pg.C_sca_115000014 NR Annotation: No match found NR E value: Ac Annotation: CNSN01-F-059701-501 (unknown) Ac E Value: 1.00E-09

147

Sequence: MKRFTLLALLLVAILYISQSESKCQGVTKCMVNLFTCKREQISVNIAADCCKEYFECFNECKPGSRPPCKNTAPGKRGSWNKRYSSRYGSDILGDIISGY* Lottia Protein Model: jgi_Lotgi1_171757_fgenesh2_pg.C_sca_119000045 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO37253.1| p redicted protein [Nematostella vectensis] NR E value: 7.00E-24 Ac Annotation: CNSN01-C-000842-501 (Unknown) Ac E Value: 2.00E-07 Sequence: MWKIFSFHWIYFLFSLFCLLHSVTCVKKVSKTPRKSKESEVSKESTKRSASLDDDPSLWPGHLQPLGSSQQVHKVDAYKKYLKPEEFLNSYAAPYLPVLFQAVEQTSIAYEKW GNITYFTVLPQTLTNW VHISPEVGQVSFRKFLLNYTDKGSVFNNKVPFFMRSAKWEPYRDDVSVPKCLHCEPILNSFSTHKLW ISHTDQSSPIHRRNKDTIHCTFVGSQEFTLISHIEYGDKVPVDYTTIDINKVDFTKFPTLREVEYHKVRVKAGDCLYIPTKW YWQVRSTGKTYSADIEW YHRNKTVKSSNCKTKTSISSLNSVVFKDIDDPTTIETDVLLHYFTTYLLNVPKFTLPQFERHLKKDKKFMEDLIEWTDELQHITKEVFETLDVNKDSRFSMADLESLSQPMLEDIRGLFLDRLQDYDDILKDQQEEAKESKKQSEKENKAKDAPATTGPVDDYLKNYIGQLEDVLKESIEEMSVTGHLPDIKKKFAEKQAQGKTGNADKPKQSTKPKTEREKAKEKLEQDRQRRKEKQEAENIKEKTKDDEKYLIVDDSVEEEIMADDDDDSTTGSTNKRTPTADKADTKDNKSHTKDNKADTRHVDKKTGRKGQDGKEAKQKNTGSKIKTEL* Lottia Protein Model: jgi_Lotgi1_173456_fgenesh2_pg.C_sca_159000027 NR Annotation: No match found NR E value: Ac Annotation: CNSN01-C-007362-501 Ac E Value: 8.00E-06 Sequence: MWSGVW CGVVYGVMWSGVW CGVWSGVERSVVW RGVWSDVDGVMWKECGVVYGVVW CMKWCGVWSDVERSVVWCMKWCGVW SDVERSVVW CMEW CGVWSGV Lottia Protein Model: jgi_Lotgi1_175543_fgenesh2_pg.C_sca_302000002 NR Annotation: No match found NR E value: Ac Annotation: PEG003-C-220719-501 (unknown) Ac E Value: 3.00E-08 Sequence: MNTMFLLVVFLLIPATSAFDSTQTNRTIVNSTITTKINGSSIGLGTCSEFTPQSCSGVCGQFFELW CSCDEYCQVYRRCCFDYEAVCKEESEKSKQDHENLMNVTVECDSNMSGVHVPQQRISPASG* Lottia Protein Model: jgi_Lotgi1_176704_fgenesh2_pg.C_sca_2718000001 NR Annotation: PREDICTED: similar to rCG46800 [Nasonia vitripennis] NR E value: 6.00E-09 Ac Annotation: CNSN01-F-096594-501 (unknown) Ac E Value: 9.00E-12 Sequence: MLTIRVLGVFAVIVLAFVLVESDVELCEDDVNVCHGELPANAMCSGENNKTKRCQCKKGYKMSLDIDCTSNGSVFSCQDIDECATNNGSCEYKCQNEPGSYNCSCPEGSELQGNGLNCGPSGP Lottia Protein Model: jgi_Lotgi1_204607_estExt_fgenesh2_kg.C_sca_690020

148

NR Annotation: ctg4a [Aedes aegypti] gb|EAT44181.1| ctg4a [Aedes aegypti] NR E value: 2.00E-45 Ac Annotation: CNSN01-F-087652-501 (unknown) Ac E Value: 2.00E-30 Sequence: MAFMFFQTLLIIVSIGYLHADDSDGVQEATLCEVCRILAEELQLRLDETGKSKEVIETGHGLDTKKKKKYNFSELRLIEALQEPNICDRILEYSVHKEKTGIERFAKGRSQTMEALHGLVNKGVKVELGMPHEMWDKPSVEITQMQRKCYALVEEYDEDIEDW YYNHQDQPFLDYFCRNLVLNPQNNECLDEDMRSQNEGSSSEMKGDKGKKEEL* Lottia Protein Model: jgi_Lotgi1_204654_estExt_fgenesh2_kg.C_sca_720011 NR Annotation: PREDICTED: hypothetical protein [Monodelphis domestica] NR E value: 8.00E-93 Ac Annotation: 190207_2814_0323 (unknown) Ac E Value: 2.00E-12 Sequence: MSLLIATGLFCVIYLLVKFLNISQPCHLPELYFKDKTSNFIQTVFTMCPILFESYIPPLLW GKSGHLQTFIYAKMGRIMSPLPNGKRIELIRPDNATMSFDVFQPHYEHPTGRDYTLAVCPGIANSSESLYIRTLVDHAQYNGFRVAVLNHLGALRKVKLTSPRIFNYGETGEYNCMIDELKRLYPNTSIIAVGCSMGANIVIKYLGECKQHENKVIGALSFCQGYDVNDAKPFLLGW ESCRRLYCYFMAINLRQLLRSHQDILFTEQAQSEYGTVELKKLYSATSLLAIDELYSVNAFKFKCCEEYYHWASCKQYIQNITKPIFILNAEDDPIVPKQLYTTPLKYQENHDNCLFIVTKHGGHLGFFEGGFIKPNTITW LDRAVVQFSNVLCELDCKERKS* Lottia Protein Model: jg i_Lotgi1_67930_gw1.39.124.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO32094.1| predicted protein [Nematostella vectensis] NR E value: 1.00E-10 Ac Annotation: 061241_2672_2657 (unknown) Ac E Value: 3.00E-11 Sequence: VGHFVLSCCMW RAVFSCCMWRVVFSCCMWRAVFSCCMWRAVFSCCMW RAIFSCCMW RAVFSCCMWRAVFSCCMWRAVFSCCMWRAVFSCCMW RAVFSCCMWQHVASCIQLLHVASCIQLLHVASCTQLKHVASCIQLLHVASCTQLKHVASCIQLLHVASCIQLLHVASCI Lottia Protein Model: jgi_Lotgi1_98330_gw1.10.599.1 NR Annotation: hypothetical protein NEMVEDRAFT_v1g147894 [Nematostella vectensis] gb|EDO28414.1| predicted protein [Nematostella vectensis] NR E value: 5.00E-06 Ac Annotation: No_Hit_[N/A]_[1]_APLCNSN01-F-143489-501 (unknown) Ac E Value: 3.00E-07 Sequence: TVCSLGTHCLSITVCSLGTHCLSITVCSLGTHCLSITVFCSLGTHCLSITVCSLGTHCLSITVCSLGTH CLSITVCSLGTHC

149

Object 2-4. Predicted Lottia secreted signa ling proteins.

Strong Annotation

Lottia Protein Model: jgi_Lotgi1_103075_e_gw1.1.1164.1 NR Annotation: PREDICTED: similar to CG31650-PC, isoform C [Apis mellifera] (Reticulocalbin 1 precursor) NR E value: 9.00E-50 Sequence: MNVYYNILILCLGVYCTLGEKEKVPNEPVDQKHTKDGEHNPHYDHEAVIGSKQLEHEFDELPPAEAIQRLENLVKHHDLNADNIITHDELKKW ILASYMSLDEEESDEKFQENDVDGNGNVSW RELVKKEFGYDLEDIEDFKKNTDKQEPDTKEFLLMLDEEEKRFMTADTDNNGNLEKQEFRAYYHPNEYPHMHGVEIERALRTHDKNKDNQVSKAEFIGGLLFLEDREVYLTEEENFNSFDLDGNGKLSQDEVRAW VIQDNNQAAEDEVLHLISQADDNKNNELTLAEILEHYDDFVGSATDYGHKLSDEL* Lottia Protein Model: jg i_Lotgi1_119019_e_gw1.30.87.1 NR Annotation: PREDICTED: similar to seleno protein N, 1 isoform 2 precursor [Canis familiaris] NR E value: 2.00E-85 Sequence: MVLFTLLCCLFIIGRHDPTQIPEEIVINIGDDGVLLFQQHDRDNDGYLSIQEFEPLVYRLLEINVSGPVYDVPISTDDEMITLKSYFIPIVKESMSKDLNDSVSIGLLRTMNSLHGLEKW QNVNLQWMNFGASHFSGFLPKDVDSIMLGSSYFIINVEKGLFNAALSSNRYYPPKVTTNESIIVHRLLTLFHPRPFVVSRFPAQSSVACVRAYNDKYLDIVFRIHAEFQLNEPPYHPFWFTPAQFTGNLIISKDGKHIQYFNLYVPNNKRLNIDMEWLNGPNESENMEVDIGFMPLMQLNSTQSSVPVKNLEEYELLEPLPERPQHKTEGENIEWMATIDLEDAKSSLEKALYPFKKVPYYNFTEAFKKAEDNKKLVHSILLW GALDDQSC* Lottia Protein Model: jg i_Lotgi1_121665_e_gw1.37.7.1 NR Annotation: hedgehog [Patella vulgata] NR E value: 1.00E-171 Sequence: MKLFFSFQSVCSWTFIFLFITSLTHACGPGRGSGSRRRPRKRTPLVFKQHVPNVSENTLGASGISDGKIRRNSEKFKNLVKNENPDIVFKNEEGDGSDYLMSRRCQDKLNSLAVSVMNNWKDVRLRVTEAWDDSPNSHAKDSLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDW VYYESRGHIHCSVKSDSSVAIKIGGCFPPTGSVQTLHGWKTMGQLTVGDKVLSINSAGQLEYSPVIAFIDRNDLEFEKYLTLHTEDDTDITLTSKHLIYASGTNSSNFESYDVVYADDIMEGDHVLITSSEKGAISPTRVVTISEKTLQGVYAPLTVNGNIVVDGVVVSCYAVVSDANLAHAVFAPMRGLHYLSQYVPW FLHSTQQENAPQNGVHW YAKMLYNIGSTFLNEKTLYIA* Lottia Protein Model: jg i_Lotgi1_159314_fgenesh2_pg.C_sca_20000054 NR Annotation: Buccalin p recursor [Contains: Buccalin-D; Buccalin-E; Buccalin -F; Buccalin-G; Buccalin-H; Buccalin-A; Buccalin-I; Buccalin-J; Buccalin-K; Buccalin-L; Buccalin-B (BUCb); Buccalin-M; Buccalin gene-predicted acidic peptide A (BGPAP A); Buccalin-N; Buccalin-O; Buccalin -P; Buccalin-Q; Buccalin-R; Buccalin-C; Buccalin-S; Buccalin gene-predicted acid ic peptide B (BGPAP B)] gb|AAB27696.2| buccalin precursor [Aplysia californica] NR E value: 3.00E-66 Sequence: MAARKHELVLVLTSVLCFVSSIVGDPNVPSDSQDNSALTQDDFAKRGMDKFGFAGGVGKRGLDKFGFTGQLGKRDMDSFGFAGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFAGQLGKRGLDQYGFTGQLGKRGLDQYGFAGQLGKRGLDHYGFAGQLGKRGLDQYGFAGQLGKRGFDQFGFAGQLGKRGLDHYGFA GQLGKRGLDQLGFTGQLGKRQMDIFGYRGQLGKRQSIDKYSFLGAGIGKRSVKNTAGIKKDDA* Lottia Protein Model: jg i_Lotgi1_163440_fgenesh2_pg.C_sca_39000174

150

NR Annotation: No match found NR E value: Sequence: MDLYGVVW LLAILQEVTSCFLTEKLPSLEGKEWFTKIRSGGSNDVIAYFDRNSVTFNDVKNTTNGETKW RCFMKIRGRYFLKNVRQGGGYLYKCMGIIVRSESAIQLEWSHVSRLADPALCAEENINLDPW LLISYKTVVNDFTSCPVSGGFDIKHAGYYSRDVRDSRGYDIGCNLMNIPMRLEFDCIAGEGAIFNFRSKNCVPDIHFNIYQNTICVAKWSNKRHHFVLLRTKTGLEFW CARFPVGIENQNETELYLYSDVACLEGEYAEEFVKFMKFDLQRVMYTTICADEYPQCKEGTCNVFSKLECQKTCGLCNPHRPPGICTFPKRMQGTYLLHSKHGNQNVTLEGRTLNIENVGQFDCIVFEDSPLRSTKTYTTMSIFQNGCRPRYTCVRLKRLGPSALRYSLAQNLVW PIKKERIGANICNEDNFRADGDPIRDTFRSYKKTGKPVIKIHPKPRFINCGMNTSYTIRAELPDGSICHGGFYQHCKNETKLRFDFHTCNSFIKPKEDFNCAGIFRTNYW ERAVLIQNVDNLNDIRCLIFNSLRPYQALIVVADECDKMASGLVDSNIRIPIMRLHIRSDVYPCKHIQLPLKDDVITNVVTTTEPIKMNVTLKESKNVNSFSVDLELSSRNSSGSRPFENKLNSLLLLFLCYVVFVKNL* Lottia Protein Model: jg i_Lotgi1_164016_fgenesh2_pg.C_sca_43000014 NR Annotation: chordin [Danio rerio] sp|O57472|CHRD_BRARE Chordin precursor (Protein chordino) gb|AAB93485.1| chordin [Danio rerio] NR E value: 1.00E-119 Sequence: MSPMDLAVCLVLLAATVSSTKFSKIPLKAARENLFGIQPNRPGCILGSKFYGI GERWTPELLPVGEMYCVKCECVPVERKGIIDMKGQTLCKNIKNLCPRPNCLNPVLPKGKCCKVCPDEVNSFEDSFSLGSKSSAPTMISSDSKRRVRNEFVSLLVGKNVRRESVKTSAVAAVYFSIVGRDEIRYSVRYFKLDRPKFLQITDANGNVLFERPIEKKRNGDKKFCGIWTKVPSAYIQYVREERLFAVITASRHFRGLVSGRIMVNRHAKREVFSSVLASPKANGIGGLLSVVYNPRTHLVDYVINLDGVFPGSSEEYFITISKKSKVLHQSKGKASSKTRFIRGSWTLKNKRQSKQLARGRLTIRLTSTNGASVSGDIKPKVTCGVSQAVMSSGNSLEEMKLASSGTAVFELREKGSLDYKIRVTGLTSKVHRIRLEGAVNKKNRRQIIGSVHRNFKADENSFNGW CNGTFKKMTADDLHLFLNNKIFVNVATSENRISELRGRVIALPYHSHFEQVSADPAALGPKTITSNGHAGNAWISMGVGCSLHYDITTTTIAKHHDLTLSLVSPTDIDAYVTIEKISTTNLYEFQQASGSIAVVPESLFRDLDEGSALIQVISGDVILSGNVSVPNTCWQYSQDYDMDILVDEQTSEEVLAANRYKCVYEGKVYENGDSW LPDVNATCNTCSCNKGKIECHRLICPVVECANPIVLDGECCPTCPIETTDQTECKIDGDLRSHPVGSTWHPFLPLMGYAKCVICTCLPGGIYKCERKCPKLDCPASRRIRLNPKDCCQVCAPEEVKPEVVKPLQDVDMKGACSFKDQIYANGAKWHPRVIPFGYMKCIDCSCNNGTPSCSRPKCPKLSCSRKIREDGACCDTCADSDGEEIVEEKKSDSCIFGGKKYDDGETFQPKTSSLSSCATCTCRKGSMKCSLTCPEKCKDPKHSNDPCCKHC* Lottia Protein Model: jg i_Lotgi1_164520_fgenesh2_pg.C_sca_45000185 NR Annotation: PREDICTED: similar to filaggrin 2 [Rattus norvegicus] (molecular regulat ion of epidermal terminal differentiat ion) NR E value: 5.00E-37 Sequence: MIKLTLSILDIVQIVKVTFSILDTVQIVKLTLSILDIVQMIKLTLSILDIVQIVKVTFSILDIVQIVKLTLSILDTVQIVKLTLSILDIVQIVKLTLSILDTVQIVKLTLSILDTVQIIKLTLSILDIVQIVKLTLSILDTVQIVKLTLSILDTVQIVKLTLSILDTVQIVKLTLSILDTVQIVKVTFSILDTVQIVKLTLSILDTVQIVKLTLSILDTVQIVKLTFSIFDTVQIVKLTLSILDIVQIVKVTFSILDTVQIVKLTLSILDIVQIVKLTFSIFDTVQIVKLTLSILDIVQIVKLTLSIHVVC* Lottia Protein Model: jgi_Lotgi1_168619_fgenesh2_pg.C_sca_77000042 NR Annotation: crossveinless [Saccoglossus kowalevskii] (a new family of twisted-gastrulation-like modulators of bmp signaling) NR E value: 1.00E-148 Sequence: MYYTELSILAKCIALCILTCFPILIQAQLTGTVTRCYNEGGEVKIQGITTDPCISCYCMDGYVQCDKKKCDNLDGCHAILFDGPKRCCDTCKGCRLNGVKHESGKSWFDKRDRCTSYSCKAGVITKSKVQCHVPCSNPIKRKR

151

QCCPSCKGCHFDGADRKNGEKFALASDPCVECKCRKGSVTCMKRACPVLNCPDEVIYQPKGECCPKCKGMRKIFDFPGACYFAKRVYVDGFSFEPKSETRCTCTKGTVICEKATCPPVACPVEERVQKGSCQVCEPKRNCLYDGKIHMHRSKWQPRMCTQCSCQNGVTYCQRERCNNSLSCPNGYKLQFQPGECCPKCVEHDAVCRVFGDPHYRTFDGKMYNFQGTCKYMLSQDCQGKDFTIKVKNGVRLSSGFAWTQMVVVLMGDTRISFRQNLLIKINRRRVQLPYTIPGKFSIRREGHSVTFRADIGLKVVW DGDSFLEVTASRKYKNRLCGLCGNYNGLETDDLIGRKGKNYLRGEEFGNSW RIGSKKACKTQPKVKNLQSICDKDFKAKVRANKECSVLYSRAFSSCRRVVDVTPYVTSCVTDMCDCPHGKKCSCESIQAYAHECKRAGHKVKW EKVSNCKAPQKNCPKGAYYSLCAPACPKTCSSNKPDGSCSKKCSPGCVCQNGAVLYKNRCISPEKCPKT* Lottia Protein Model: jgi_Lotgi1_179503_fgenesh2_pm.C_sca_71000002 NR Annotation: Wnt7 [Euprymna scolopes] NR E value: 1.00E-122 Sequence: MTVSPTVVWAAFSMALSSVVALGANIICNKIPGMAPKQRAICRSRPDAIVSIGEGAKLGLTECQYQFRFMRWNCSTLDSNDSMFGYESLGGTKEAAFIYAMTSAGVSYAITQSCGLGSLPNCGCDKDKGDGKLAPQGWKWGGCSADIKHGLRLARKFMDAREIAQNARSLMNLHNNRAGRKAVKDNMGTDCKCHGVSGSCTMKTCWTTLPPFRKIGDSLKKRYKKSKIVVPYLGRRARTAVTLILKRAKRPHRKPRRSHLVYLDKSPNYCDFDGKTGSLGTVGRKCNRTTKDTDGCDLMCCGRGYNTHQYTRTWQCNCKFHW CCYVNCNKCSERTEEYTCK* Lottia Protein Model: jgi_Lotgi1_186451_estExt_Genewise1.C_sca_160091 NR Annotation: PREDICTED: similar to out at first [Nasonia vitripennis] (Vital for proper neuronal development and hatching) NR E value: 7.00E-69 Sequence: MSMSLIPSLSYSIFVCVLIKICHTQLVVNVKNGGGDILVESIQSNVSSDTITLEYPSSDGSLITQFIDFKSEAQIFRAYVPGEEERGQPPGSVQVLCFITRFSKNDFISSDAMSKLRQKNPTAIRTPEEERPQEVHSFDLSVDLNKSHVISPHVFNICREAKESTFFNEDDLKTISRSLSKDYNTMMSAMIKLAPTKYGKCSDTSDIFKPCTCRYDACVGWYPCGLKYCRGKDSSGKVVSYRCGIKTCKRCLAFDHFVKQKFLCLWN Lottia Protein Model: jgi_Lotgi1_188920_estExt_Genewise1.C_sca_260301 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO37057.1| p redicted protein [Nematostella vectensis] (growth factor) NR E value: 1.00E-109 Sequence: MFLEVFNISATVLCILFLIGHRKCDTIQNSKHHAELMMDLLQPYKNYHGQRLKKSNRHIRECQEIKYGNVTHSKVKVKPDTNISYPLVEVKHLTKEIGSYYYKNPVTIRYQRVDFPLNTLSVLEPGEPGTCREGHGTRSITSKTALSGNCIVGVNGGFFNTTTGACLGNIVSNGRLVQDSQGLQNAHFGITEDGYIYVGYLSELDLITQQFKQLLGGVLW LIRDGEKYIDKSKQMECPDVEETGTIETFIEVQSARSAVGHDKDGRVIMVQVDGKTNARGVNLHEFTDILLELGVVNAINLDGGGSTTTVINHTLVNYPSDKCADGTFSCEREISTILCVHEPECQPNDCNQHGHCVLGKCVCDGYW VGSDCSSLSCPFSCSHHGTCTKDGCICDDGW YGINCSTPCKPGYYGSNCAHQCYCLNHGTCNSQTGHCVCSPGFTGKYCESLCEFGYYGDKCNNLCTCDNGCFCHHVTGSCNLS Lottia Protein Model: jgi_Lotgi1_205842_estExt_fgenesh2_pm.C_sca_70027 NR Annotation: BMP2/4 [Patella vulgata] NR E value: 1.00E-159 Sequence: MIADFYRSVVLLLLVLVVNYTSSLSTNTLHPRKHGNEKVLAAVESNLLGLFGLKSRPVPGRRTPIPDYMLDLYKRLDSEPDFISPFAESKGKGIVEANTVRSFYHSDINFVKDCDERTCARIWFNISTIPFAEIMAASELRVFKDVYNHFKLTDANSLSGDRIKASLFKHRLEIHEIMRVSPDDSECISRLIDTKIVDTRNSSWESFDVHPAVLKWRRRPQYNYGLEVRIVSKSPWISTNSHVRLRRSAHMDEKNWQIQRPILVTYTHDGRNPNSRIKRASARNRRRKSRKRRRRKKKGNKNECKRHALYVDFGDVGW NDWIVAPTGYNAYFCRGECPFPMGQHLNSTHHAVMQTLVHSVNPSAVPKACCVPSELSAISMLYLDEW DKVVLKNYQDMVVEGCGCR*

152

Lottia Protein Model: jgi_Lotgi1_208417_estExt_Genewise1Plus.C_sca_10710 NR Annotation: fasciclin- like protein NR E value: 5e-178 Sequence: MSRNLWIFLLILLSIIVIASSRRSRGRW YRGRSGRRSLADRLRDRGTKPSNHRPRQRHGW GRW GSGW GAGQDHRTRVEEELDDLFRRIKDRRYGFNFEDKFW LDFGWNSRNEPKPWWEGPNVCKDEKESIENTSISDTGPISRHFSFKNQVCDQSETSYKCTTKTGIFMKQKTVTEVYECCPGFTRKDEMFGCPTEFKLDGLVKTARNLGLTEFLRATESVSLTDDLEKGNFTVFAPVNKAFEDEKNILPELETILPKDMPSVVMVSKPLTDLVIA DMSNVLLGHLVYDTLKTSGLNDEQLIETASPFDSLLRVNFFSRPEKLITVNCARILSADNQATNGIIHTVDRIIKPVTDSIVDIISQNPNLSYLKTALARADLVKALRGTGQYTLFAPTDAAFEKLEPSIRDRLIKGEKSCLEKVLKNHLLTSVICSTVIQGTVTTTNLLNKYLNLTRTEDDKLFVEGSQMVGRDIMATNGVIHVIEDVLVPDQALGLIDVAEKNNLTKFVELLKETGMFKGITKMENLTLFIPSNNAFEKLEEKVSLEELKKDKKKVEDIVMYHVVPEVVTCSRLHNNYKLSTENERNIRMNRYSTFPFRHHSIQTAQCAPIIKENVPACNGVINIVNEVLLPPVGNVVDNLASKKKFSTLVRLMKKSGLADMLQEEGPFTMFAPTNEAFDDLYEEDLEKIEDNPEVLKKVLQNHIVQEFVCCASISMVRSWFDVASVRTLSGVRFPVKERHGTIQFNHAVVTECDNTATNGVVHAIDKVTLSPERPFWKRDFYHW* Weak Annotation

Lottia Protein Model: jg i_Lotgi1_110650_e_gw1.12.27.1 NR Annotation: unnamed protein product [Tetraodon nigrovirid is] NR E value: 1.00E-32 Sequence: MKIILVLLCFCHLIYSAKTCPSNCQCHLTDKAVNCYKRELQFVPANIPLDTEILDLSSNDIANIDPTMFHNLTKLTDLVLNDNKISKLDPRTFQNLHKLEVIKLSQNFLSTIPEGLFDNLPIMVPKLMELNLSQNKIVKIENYVFRPLSMLSTVSLNDNPLKNADRLLSKNRRLSYIDMSECQLTTVPRGLPDSIEHLKLIKNNITKIKPKDFRNKNYLKILALDENEIEFIAPNSFKSLQKLIQLW LNGNRLRKFPSPISPSVTNVYLAKNNITKLSAADF Lottia Protein Model: jg i_Lotgi1_113861_e_gw1.18.363.1 NR Annotation: PREDICTED: similar to tartan CG11280-PA [Apis mellifera] NR E value: 5.00E-27 Sequence: MVKPPTQNTRWWIIVWILLFQKYTCEPEVTCPHGCKCDVDVVNCLHSNLTSVPVGLSNGDRLQELKLGRNKITEIKLELLEYKELRLLDLISNNISTIDGSAFRTLYKLETLFLSRNFITKTDKNTFRNLTSLRFLDLSYNNLRCLKIGNFEDLQSLVNLDLSRNQISLIEAESFRCLENLEILKLPRNQLDQFPW KIFQNLTSLKMLVLDDNLLTSLPISAFSNLINLEYLSLTGNRLHELLPSSFMMSDEGAISLEQLYLRGTDLDTIPVKLFAKLKKLTVLDLSKTQIVSVKNENLNGLSSLESFIMTSSNNLTYIEKGVFQDLHSLRKIDISGNTLLSEIQFTTSSLRNLSLFDLSANSL Lottia Protein Model: jg i_Lotgi1_137329_e_gw1.113.40.1 NR Annotation: insulin-like growth factor b inding protein, acid labile subunit [Sus scrofa] gb|ABE73450.1| acid-labile subunit [Sus scrofa] gb|ABE73451.1| acid-lab ile subunit [Sus scrofa] NR E value: 1.00E-21 Sequence: MSFVQLLPFFLFLTISQSSSQRWPLSGTCYPCSCDINQKGFGRVKYVNCSNLALTRIPRNLPLDLHTLDFSNNRILPQEIQQLCTFNSMQYVALSYNSLDSIPPEIFKDLENLHTLVLHGISSIPTTNIFEDLFNLEYLDINDCHVNQIPDNWFRKLYSLKTLKLRQTGIRSIEPGVLDGLLNLQELDLSHNVMRTAQTESFKPIVQSIRRINFRGNMFKTIGDHLFEKMYNLVELDLSDNKLESIHKRSFLDLRQLINLDIRDNKLSNLPGGLFKNLRNLQTLNLAMNTFNNFPETLFKIGYLLKLDLSYNRIRRLPDSFVHSFPYLEFLNVDKNPLHCDCKSLDVKFYQPNLVII Lottia Protein Model: jg i_Lotgi1_152641_fgenesh2_pg.C_sca_2000061 NR Annotation: interleukin 17 [Crassostrea gigas]

153

NR E value: 5.00E-07 Sequence: MVLMKVFICALLVNMVYSFTLIKNIEHILERERRSDECAVPNNIDELFTNLNSHVNRVEFLIAVNATHQPNAQGLIPPAAGDQTDTECPKEYTGKDTSNSGIW QRSTCPWFYSERNYGAEYYPSKVYNAACKCVDCLYSKSNSETGCSRIYRNINILKKTGCSNGFYTYEQRSISVSVGCTCAKLQANIG* Lottia Protein Model: jg i_Lotgi1_157019_fgenesh2_pg.C_sca_12000330 NR Annotation: No match found NR E value : Comment: Similar to Chordin Sequence: MFSLVLLVVTPWLVFCMPPPPPAMCTCSDGTQVPPGGTHSISDCHSCYCDENNSEAQEIIAACLIVPCVDSVKMPGQCCPTCPNGENCRAEFKDDNGVERSEIIKVSDGVVKFNSTECQCDYQNYNGEPKANCVQVLSIEPVIAEKSTP* Lottia Protein Model: jg i_Lotgi1_167943_fgenesh2_pg.C_sca_71000005 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO34356.1| p redicted protein [Nematostella vectensis] NR E value: 1.00E-21 Sequence: MKSILVTSVVVVAGTLLTLVQALGADKFAEPATQKHSKIPTAALSHSGDTLLNTDAHQTNPSNIWKSKSKFDLDALPVVNRQDVQSHYAQHRRSYDTPMFLGKRDLKNLSLRFKRATDLEDLDATEFSDDFDKRGVDIPQFIGRRRYDTPQFIGRRGYGVPAFVGKRAFRAPAFIGKKAFGAPAFIGKKAFDAPSFVGKRKLTAPSFIGKRGLDVPAFVGRRAYAPPQFIGRRGMDVPAFVGKREFQTPMFLGKRAFSAPSFVGKRGLDAPTFIGKRKEESFRDLLATLQAYREYRRLMQTDKRFDAPLFVGKRSDYENDNLAAETLSRRFQCIPQW VFRMRLNVVDLNMSFIGGYKQNYFSLNILDDVQTKILLPLDDLHKVDSSKMDVPTVQLWDADMPRPNGARFLKLDPAHALGFLGQEASPVSKRYLEFVGKRDNDKRYAEFVGKRAGFGEKRYSRGSLSEYLSKRLRIRRPEFVGKRSNQKRYLEFIGR* Lottia Protein Model: jg i_Lotgi1_168377_fgenesh2_pg.C_sca_74000087 NR Annotation: TBL-1 NR E value: 2.00E-07 Sequence: MLPHITQCVILFITFLCDISVLLPSRDGGRGLTYAQLVPHRTSNDVYMDPCKAAGFMGDIALSEEEFKRERKYLQQMNNTMFELQVEDPRTTYIGRKPSKYSGQPENTLKKLPMSELDVKKKKYLRKILKEKKQNLKSLCKKGFQTVVYTRQVPVGFKFISPFLYLLDLNIL* Lottia Protein Model: jgi_Lotgi1_168508_fgenesh2_pg.C_sca_76000035 NR Annotation: ependymin-related protein precursor; EpenHg precursor [Holothuria glaberrima] NR E value: 3.00E-10 Sequence: MMKYCLVVAVVVVGVVIGEPSKPGCCTPSQWEGQQGSTIGIVDGGIPSVRTSMVRISYDEKNQRIAYVTSESTPQGEKNYKIIYDYSRKFQFIVDLDTKHCTVSSLTDPFMKLCLPVDAQPKGKFYYGIDGNNLPAMAFSLNVNGTMITVVTTADNCIPIAEVLVDGQAQGVSQMHASGFVNLTSGIKDPSVFIPPGTCTQ Lottia Protein Model: jgi_Lotgi1_169325_fgenesh2_pg.C_sca_84000054 NR Annotation: Insulin precursor [Contains: Insulin B chain; Insulin B chain'; Insulin A chain] gb|AAF80383.1|AF160192_1 insulin precursor [Aplysia californica] NR E value: 4.00E-13

154

Sequence: MEVTCKCPLVLLGVLFLNFGTVLTHLEWTCTLETKRESPRGVCGQRLPEVLSMVCKRYGGYRDTWFRKRNGEGTNSRLGNIILGKRDAFSYLGKRGQSYGEQGITCECCYHSCSFRELRQYCRNSQQRISIKK*

155

Object 2-5. Controversial predicted Lottia secreted signaling proteins.

All software programs are inherently flawed in that they can only use those parameters

they are programmed with to make predictions about whether a protein may or may not be a

secreted signaling molecules. Due to these flaws, our final list of predicted secreted signaling

molecules was manually screened for conserved motifs, signal peptides, and annotated to make

the most conservative list of predicted neurosecretory products. Here, we show those transcripts

predicted to be secretory molecules due to targeting to the classical signaling cascade but are

either unlikely to be secreted outside of the cell, or unlikely to serve as neurosecretory signaling

molecules. Some of these predicted secretory molecules may be part of the secretory apparatus

but do not encode for signaling peptides.

Furthermore, many of these predicted molecules have motifs of non-secretory products but

may be secretory in our model species. Further experimentation is needed to determine the

functional role of these products, so we have decided to leave these products in this supplemental

section to provide a complete unbiased review of all predicted products for future researchers.

Annotated

Lottia Protein Model: jgi_Lotgi1_108564_e_gw1.8.40.1 NR Annotation: PREDICTED: hypothetical protein [St rongylocentrotus purpuratus] ref|XP_001179636.1| PREDICTED: hypothetical protein [Strongylocentrotus purpuratus] NR E value: 2.00E-86 Sequence: MYLSCYLPVILLFIEDLKQAVVAMMLRKDEVEEKNKSLKAMLDREMEISSTLRAEIEEMKISFKLSKDKEIAKNETLQKENELLKHQLRKYINAVQLLRTEGAKDDTQGITLEDPQPIIPPAKPSIDYSHEASEYEKKLIQVAEMHGELMEFNELLHRQINCKEAVIRNLKEELTDLRGPLPYDAQSSDDSLSGDLESSLISRCLINIWIPSAFLGGSKTDSHHVYQVYVRIRDEEW NVYRRYSKFLDVHTRLKKVYPLIEKFEFPPKKTIGSKDPKVVTARRKMLQSYLRKVINHLLEKNADLSSNVSKEKLIAVLPFFK* Lottia Protein Model: jg i_Lotgi1_118077_e_gw1.28.72.1 NR Annotation: LOC100124858 protein [Xenopus tropicalis] NR E value: 1.00E-108 Sequence: MGSTLNILVILGCILVPFSFSNFLEW GPDLEGPW CATRPIEQCCPGRDDECTVPILGTKCYCDIFCNETAEDCCPDFW NLCLGVTRPTPWPLTTTTSRVPINILCNVYW FKCTKLHFSSHWECSNDDCLLEADHITQINNGPYSWVASNYSDFW GLTLEDGVKYRLGTFPLGSNVVQMTPLRVKLTDVLPESFDARTKWPSYIKPIRDQGNCGA

156

SWAFSTTAVASDRLAIESLGEIKDELSAQHMLSCNVRKQKGCEGGNLDRAWWFLRKTGVVTEECYPYES GDTTDKGDCLVSRRKSNTTCPSRILYKEKRRYKATPPYRIAPKEREIMKEIMDNGPVQATFMVKSDFFMYKNGVYRYSNISFDEEPAVYHSVRIIGW GVERTIYGDILKYWICANSW GTQW GENGYFRIIRGRDECEIESYIVGVW GQISGDASLRTLLTRGRRRRLFAERSLRNLRVNSMAKGRKSRSRQVGRKKVKRGRKSREERRRLRQEHRNKRKLRRQSRKQDSDSQNTV* Lottia Protein Model: jg i_Lotgi1_134336_e_gw1.89.95.1 NR Annotation: apolipoprotein D [Branchiostoma belcheri tsingtauense] NR E value: 9.00E-41 Ac Annotation: CNSN01-F-078834-501 Ac E Value: 2.00E-25 Sequence: MERILTAVLLCIGVAVYAQVPGLGPCFDAKPQSTLNVSQYLGHW YEITKLPSFFEIEQVCLTANYSVKEDGHIKVFNQGKVNGKNVSSTGDAYVPDPSEPSKLIVKFPQAAPYGPYW VLDTDYTSYSLVFSCEQLGSFAHAKFLWILSRTPSIDPAVKDRLFKRLEKDGIGASGLRATKRSDCS* Lottia Protein Model: jg i_Lotgi1_154590_fgenesh2_pg.C_sca_6000093 NR Annotation: PREDICTED: similar to meteorin, g lial cell differentiation regulator-like [Tribolium castaneum] NR E value: 3.00E-24 Comments: Secreted, involved in both glial cell differentiat ion and axonal network fo rmation during neurogenesis. Sequence: MDFLQATWLLVVMVIFHIYIITADQICNQCDCTIRDSTGKGNINVKPKCQSGKITWPDPVSAVRLELKPEVVGPFKACIALKSENIVTLVSREITTETVNYRTRSYDSKVDLLPWFSVEGQGKEHCVTSSESSVMLYLEIESTTEDSGLSRTFIYYDIELIPDNKFSPDPMEECQPCSDDEILRSYCISDYVVVGRMKEVNFQSELDKTKVNIIVDQIVKTTDEDIFKRHDNLLEGALFTSVHCGVQKGPGQFLFTGRNRLGQPILTCAPFYDQWIQIQRLAQEKGTIECAYG* Lottia Protein Model: jg i_Lotgi1_160631_fgenesh2_pg.C_sca_25000236 NR Annotation: Substrate-specific endoprotease Tex31 precursor emb|CAD36507.1| substrate-specific endoprotease [Conus textile] NR E value: 4.00E-29 Sequence: MNRLCFLLVVW QIALSQSKVVSSVEGFNGNLLRIVRSANNAAPQGNLNYNSNCNEAYKNISSSHSMCLVDVCTAVALSQEEIDAAVAAHNDYRKNVSPKAAAMQKMVW DDELAEIATKWAQQGRAGHDTKDARKTISLKGTYIGQNAAAGQANLIAAIKAWHDEVVDFKYGVGSINGKDVGHYTQVVHHQSTRVGCGKAHCPDSTYKSYYVCNYAIGQMGLTHPYKSGSSSCSDCPSSCSDNLCDCNGKICFNGGTLDINTCTCSCPSLYSGDQCQTLQCTKTEKSWCRRSYTVADCTKYSNFPIDCNMMCGVCPYPCDGKQCENGGTLDPNTCQCTCKDPYTGPTCADKSCPGQEEWWCQKFFTAADCTKYSNFPTDCNIMCGVCPACKITA* Lottia Protein Model: jg i_Lotgi1_163300_fgenesh2_pg.C_sca_39000034 NR Annotation: PREDICTED: similar to TTC17 protein, partial [Bos taurus] NR E value: 1.00E-133 Sequence: MASSVVVIVLLHFLFEQCLLTRGSMHW VVTEDGRIQSQADSVYNLRRPYDLVAFMHQEDRATVLNHLKKELLNRKGEIDKSEDRDTTGLEQKFYKSDYDCLQAGTPLPEIDLYISTILPIEHKHISDELSIEIQKAAQSSDNKIGTIPPEPDCTKVLQLDFTPHGFEHLEGVQERMNLTGSAELGLTHAIPFIPDKTEIPDFGHVIAKELKKNSTSWVLYNMAAFYW RVKGDPWMTVECLRRALHFSPRENKDIALISLGNILHRARYSNEAAIVVHSALELSKELNVNHFTLGNIYAVLGEYNKSIICYENTLRIQPDFEAATIRKHAVLCHQKLEAALEAQHRSLQKTLRDLKDYQKKHDLWHIQNDRMSVEQVSQEEKVSQNIAFEFNKAKQTSTKDIGEYCNMVEREGKQVLLCTWNRQAPTLEMLDQFALEEQKKESERNRLKLNKYEKKAIDYNLPVRAPLYVKHDRQAVPRFNELELDNTWPEKEECDSHLLTSPDPFNLSTVYLSPENKGFEVKALLTEAQNLKDGAEHPLPW YPPICVTLLTINEYDEKTYDNLKSVGHP

157

GRTKVPLKMYDPSMRKTLLSHVNGGTVTEEEVGQRILSAIKQEVGARW VLFNLAGLYW RIVGNNYHGIECIRRSLALAPDEYADVPLVNMANILYKW GRYDDAIVLMKDALKINDAEPDSNFLMANLLW VTKNYSGAISHYRTVLDIIPEHQEAFDSLRAIKCLQKFLIKEQSVAPIEVPSQPVGNSCQQKGVANKNQQTESRVICKTENGEEKCIIETRMRGKSGECNGHCTQTCTITPIKVESSCGGPDLAVDTSNPVQCHQKGSQCGEAGEELLFTNDFTNKLDEVSDHYEKKGICNGEDCNTLRVQCLIPMKTHSGLVAHVITPPKLFVRPLSLHSTQCMTEQKKPHIKLEFIDGVLHQKLIFVQDANDIHVEDNECIIFNDGIKSPGCDQAQYRSYSEELARSNINVEFRYVGDESPVEKDKKNHCSKTPNSPGVPSDEIPYSDISDGILQSLNGDVEPSEVVGHRIALTLQQNPKSW VAASAAGLYW RVEGDFTKALDCYRLSLSYAPLEMADLMLLGYANILVQGGYYSNAIMVEDLALDIQSTLPIHYFTLGNIYAALGKWRTAVSFYEATRFYQEDFKPAVDRLRAIHCENIA* Lottia Protein Model: jgi_Lotgi1_168805_fgenesh2_pg.C_sca_79000073 NR Annotation: PREDICTED: similar to plexin A CG11081-PA, isoform A [Apis mellifera] (a neuronal semaphorin receptor that controls axon guidance) NR E value: 1.00E-121 Sequence: MEGGIRLQLFLLVSLICHTQCIKILHSFRDPGGNILQKLVIDDQTGNVFLGALNRLHKLDPDLNIVQSASTGPRLDNVECPPPLLPCDKPKTQLNSQIKGLVIDSASNSLILCSSLFHGSCQTLALNNITNVKKFFHKPLVPNDHHSCYMFLAPSINNTQALYIATAYSDLGDAAYRDLVPSISSRSLDNLDFIHRDTEGSTKLEILKEYRESFIVRQLYGFQNGGFVYFITLQRENPGSQKIVSRINRLCQQDRYFRSFVEIPIKCQKSDSDSYDVVQNAYFTNGKLYVIFSNTVSSAGAKKSTFCEYDIQDIDLEFNETVRNCYKAVGNFGPEHYKKIEPCPPLIFGSVDFCGESGEW KDYSAIEGSKPISATPLHFFTSDKPTAIFVHPEGNHRYAYIGTQDGHVIKLRFENGQVNQLFKITHSVGDIILQITNHPQSNLLYILGSHKLSVLSLNHCDDLTSCDECMNDGDETCGW CVMDNRCTTKELCQSSVITPPW LSRTDQSCASISSLQPSSLSYKKFINGGDSRQIKFQVNKLKFESNRNLNCLFINGEKHDNTMAILDQKENTITCPLPRQLPTIPQGKDHEDLELQFHVEGKMIVKRDVSVFDCQGNNNCTSCSTSQFNCHWCPVSHSCVEQSESCPQGDSIKVNDRCPRLETAASDTDILVHSGELKTISVQVRALEANQRSNLKCHFDLSGQVQTVSAKISSSTLTCETIKLAYADDIPYIVAGFKVTW GQQNHPLDNPQRMNVRIYKCASMVTNCGKCLSLDVEYECGWCEDQCSLQKKCQKSWLDQSETCPNPQILRYSPSKGPVKGKTEIQVNGINLGKEISDILGQVTVAGLPCDVMQEHYEPSSSFKCEVKGVTSAKSGTVKVVVNNKYTAESDTVFEFVDPVLNHVDPKQGLMSGGTRVLIKGNNLDVGSDTQVIVGGNSVPVIK* Lottia Protein Model: jg i_Lotgi1_192261_estExt_Genewise1.C_sca_450209 NR Annotation: conserved hypothetical protein [Aedes aegypti] gb|EAT46740.1| conserved hypothetical protein [Aedes aegypti] NR E value: 1.00E-128 Sequence: MTLYITSLVFFLFINGTLQVKNRSSKVADRCFCKLNGEIDDCSCKVETLSVLNNNKIYPRIKSLLSRNYFRYFKVNLKKKCPFWHDDSRCALKDCHVDTCKEEEIPSAIKKGSSAYRYSEEAQKEESCAEEKELSALNTTISDESIQAFKDW KEHDESQDLFCDIDDESSADSEYVDLLLNPERYTGYKGESPHRIW RSIYQENCFKPQTEYIYGPSKSSSPLCLEKRAFHRLISGLHTSINIHLCADYLYQENLGYGMKAHWKPNVAEFQKRFDPQTSNGEGPQRLKNLFFTYLVELRAIAKAAPYLQEEDFFTADSDEDKDVRQGIDDLLNIISTFPDHFDESKLFAGNPNEAKLLKDEFRNHFRNVSRIMDCVGCDKCKLW GKLQTQGMGTALKILFSGDDIGPDSTVNAQQKKHFQLTRSEIVTLFNAFGRLSKSIHSLEMFKSLLSKS* Lottia Protein Model: jgi_Lotgi1_197084_estExt_Genewise1.C_sca_820083 NR Annotation: glucose-regulated protein 94 [Crassostre NR E value: 0 Sequence: MKRFIVLGILGFLFIAGAGLLNVQAEEVVTEPDVVEDIGKSREASRTDDEAVQREEEAIKLDGLNVAQMKELREKAEKFAFEAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRFVSLTDKSAMAATDELSIKIKADKASHVIQITDTGIGMTKDDLVKNLGTIAKSGTSDFLSKLGESKDQAQMSDLIGQFGVGFYSAFLVADRIVVTSKHNDDDQYVW ESDSSSFSVA KDPRGNTLGRGTMITLHLKEEAHDYLEESTIRSLVKKYSQFINFDIFLWASKTEEVEEPIEEEEEEKKEEEKKEDEDAEVEEEKEEKPKTKKVSKTIWDW ELINNVKPIWTRKPSEITDDEYNDFYKSISSDYEKPMTKIHFTAEGEVTFKSILYVPNSSPSDTFQNYGKQMEQIKMYVRRVFITDDFEDMMPKYLS

158

FIRGVVDSDDLPLNVSRETLQQHKLLKVIKKKLVRKTLDMIKKIDKDTYEKFW KEFSTNIKLGVIEDTTNRTRLAKLLRFSSSNSDTELTSLAEYLERMKEKQEHIYFVAGHNRAEVEKSPFVERLLKKGYEVLYLTEPVDEYCIQSLPEFESKKFQNVAKEGLKLDDSEKAKERKEATEKEFEPLITWLKDSALNDKIEKATISERLASSPCALVASQYGWSGNMERIMQAQAYAKAKDPSQEFYATQKKTLEINPRHPLIKELKKRVEANKEDETAKDLAVVMFETATVRSGYRITDTAGFAQRVENMLRLSLEVPLDAKIEEEPEEEPEEAEEEKTEEVEAEAEEEAEEKKSEHEEL* Lottia Protein Model: jg i_Lotgi1_204770_estExt_fgenesh2_kg.C_sca_820009 NR Annotation: unnamed protein p roduct [Tetraodon nigroviridis] NR E value: 3.00E-35 Sequence: MAATTHKMFLVACLVFLPIFASADTCLGPSVSAETYTTSEVTVSTETVFIAQFTLTCKNGLQNVNLYAEVAGRMIPAVKTSKPNEYQISITDEYKALRSGTYEAKFYDEESFSALKKAKRNNEDSNSVTPLFSINISHSGLWQGSYIQSEFVAACVAILVWW LAFSAKSKLSP* Lottia Protein Model: jg i_Lotgi1_211937_estExt_Genewise1Plus.C_sca_110124 NR Annotation: PREDICTED: similar to SPFH domain family, member 1 [Monodelphis domestica] NR E value: 1.00E-124 Sequence: MANPALALLSVSAVALAIFLNFSIHKIEEGHVGVYYRGGALLSSTSEPGYHFMLPFLTTFRSVQTTLQTDEVKNVPCGTSGGVIIYFERIEVVNKLDRAIVYETVRNYTADYDKTLIYNKIHHELNQFCSVHNLQEVYIDLFDQIDENLKKALQLDLNVMAPGLMVQAVRVTKPKIPESIRKNYEAMEGEKTKLLIAIQKQKLVEKEAETERKKAVIEAEKVALVAKIQW EQKVMEKESEKRMSEIEDSANTARAKAKADAEFYKTQKEIESNKLKLTKEFLEFTKYESISKNTKIYFGNSIPGIFSDKVEPQEIAKVVGERK* Lottia Protein Model: jg i_Lotgi1_116422_e_gw1.24.410.1 NR Annotation: PREDICTED: similar to CG14764-PA [Apis mellifera] NR E value: 7.00E-58 Sequence: MKTKNFYILVLLISVTSVALW LVSYPSPTFRNIMSTTQNNIRKIPKHMQMSKSPDLEVDKKYLDLLGFDSESKPKNDIVVIGSALMPADTENTILFLKSAQEYVPNLKLVLFDMGLSKADKQTLTNCRNETKHCEIRKFNFDQYPSHISDFANKSYKPICIQLLLKEFGTVIWADTSELFASSDIDNTMKQAKTEGLVAWTIEDATSSLTHPKMFDYFKTDQKKYFFHHAVQTSHIILVNTDKIHENVMLPW VKCALVEECINPMGAQNSGCNYQRKPKFRYTGCHWYDMSALNVILGLAFDFDEAYSGKDKIFWTKFDQEKAKAENMTDGAVHKVSVPHRVYF* Lottia Protein Model: jgi_Lotgi1_118302_e_gw1.28.365.1 NR Annotation: No match found NR E value: Sequence: CVMLYMLCKCSSLDCVKLYVLCECSICHCVKLYVLCECSICHCVKLYVLCECSICHCVKLYVLCECSICHCVKLYCSICHCVKLYVICECSICHCVKLYMFCECNICHCVKLYVLCEV* Lottia Protein Model: jg i_Lotgi1_149784_fgenesh2_kg.C_sca_15000101 NR Annotation: PREDICTED: similar to chromosome 19 open reading frame 10 [Canis familiaris] NR E value: 9.00E-20 Sequence: MLISNDFKCLFMLILGFIQW VSSYEMTRDYEEASHFDVMPGGAVLSHEKQLDGFTCKFTYACQGGTKEGWQLTINTNTDKTKYLCSVQRPDGKSYLFFQSFKLEFTGLEVKEGYAMGANFKPLSPSEYQVDFQKHSISDVAGKFGSQLEKVELYGEKGKAEL*

159

Lottia Protein Model: jg i_Lotgi1_152847_fgenesh2_pg.C_sca_2000267 NR Annotation: SARP-19 precursor [Littorina littorea] NR E value: 5.00E-08 Ac Annotation: CNSN01-F-148317-501 Ac E Value: 2.00E-07 Sequence: MIAFICVLGFISGCLAAPCTHTNSAEHTEEQVIARVIALADHNNDGILSVTDLAAYFVINFDHNHNSEVDKNEFILQWHETFHDEKAFAEHVFTHFDTNADNVLNLSDLFALNIRLDTDGNGTVTNKELESYLQLIYNAC* Lottia Protein Model: jg i_Lotgi1_158797_fgenesh2_pg.C_sca_18000147 NR Annotation: predicted protein [Monosiga brevicollis MX1] NR E value: 5.00E-92 Sequence: MKIHILAVLFSFFSATNAATLRFVSQTPLSITINKDGSYTVNVNGEPW LESSAIYFYAEGRRVSMGDGSLKIASTFMDNGYDSVGQYGKTCW RFEIESKYIDTCFKQYMEPTPFIIFEQHYLNDTENTASIDYDYTIAGFPAFTAPPTGKLGYLHYAGSMTGDSDKSFGIWQEKAKMLTGVAGGPLVLFDKDADDCLILSPLNQFMAASSLFDGQNYSTGVLGGVNFVPKDFVLQTVLFYGKGINHAMSDW GLLLTQYYGKDDSYRKSDLTLNYIGYW TDGGAYYYYNTEPNKTYQQTILDVRQNAKDIGMPYKFVQYDSFFYYKGPHGGVKTW VPMPDLFPDGFQYLYNQTGW PAGAHNRWWSPKTTYARQNGGNFDFIVEEAKAVPTDQAFWDYLFDSSKKW GLTLYEQDW LDNEFAGVKALLTQIDLGRTW LMQMGRAAQENGITIQYCMSNPRHIMQSVEIPVVTQARASGDYRAGKDNW KIGISSIMVDALGVAPFKDNFWSSPDQPGNPAYPDLKETHADLQVAVSTLSTAPVAPGDMLNKTNVPLVMQCCNADGMLLQPSKPAKAIDAQILQLAFGGDVGPQGDIWTTFSNIAGFYFGIILGTDMTADYNLTPSKASFKAFLPSEVFPRFASIPKSQEFSASNPLVITTNCTKTNICLYYTSPVFSPGGTNVLIYGEENKIVPMSPKRVTDITLDRDLKLTLTGAAKESVRFGFILPDQYITITCQLGQSGSAVLSFNERSCNGY* Lottia Protein Model: jg i_Lotgi1_159188_fgenesh2_pg.C_sca_19000225 NR Annotation: predicted protein [ Nematostella vectensis] gb|EDO44886.1| predicted protein [Nematostella vectensis] NR E value: 2e-07 Sequence: MRGPTWSVILLLINLIVTLQACDDVTEFQCADNNQCISKTDTCNTINDCDDGSDENNCACLEKLGMEDGTILDSSLIASSSKTGSSPSYARASNDKGNW CPKSDDSKPFFKVKFDTPRAISAIVFTLKQSVVDPLNYLSTWTLKAVLPGSDEEVTLYEGLNAFSNNSLYRSLFFPSLVTDTFIIAPTTW QNEPCLSFELYGCNIDNLCPTGCQNNGKCSGNDICACADGYFGTKCQFQKTTIPFSSYIFKSLESGSLVTSSETFTVRTSTTIRTSKIIEIQGRKVLSLPVADYFTAPKDGKFGCLSKLGSGGCKSFYLALGCELEELKDTVQLFTTGSDSTYIGAEATLKSDNLVVTFRTSELIWTLNVRKTNMFSSSIKYVLEFDWDISTGIRIYVNKKLIGQQRQATRTTQVLRTNTLATSGVEFFKSKSVNVKLFSLKSSSVNRISLIKTSLISLTPTEECDQEKEHKCANSDKCIDKSNVCDSVEDCDDGSDEQNCPTTTCDVKVYKKLVTKSASLSLDLLEVDDAQRVAKCTLPVISYVVDGILLDFLSKNQYGDQLSATNDLGANQNHNNNNKDDNNNNKKSSQDEFKEDVFNWADEPWTPCDDVEVPDWARMDDPTFDNFNSELIWAEDPWTQLPLVGLPSWMLLPGIDYPVEAKDPVVYTPVNDEDDAAFLASGIDIVNWSDEPYFQLPLDVPRWALLPGIDYPVRHGYNSPYWTYKLNQYNSSRREFGKKMFARQFPTLREERVLYNNNGQSKLCIPGNFQRRNHRF* Lottia Protein Model: jg i_Lotgi1_153437_fgenesh2_pg.C_sca_3000340 NR Annotation: hypothetical protein PB105991.00.0 [Plas modium berghei strain ANKA] emb|CAI04701.1| conserved hypothetical protein [Plas modium berghei] NR E value: 8.00E-09 Sequence: MKTMVAIIGVIIVSCVLHTSGLPLFKNILNERSVEYGGESGNGFKNERAVGYGGESGNGFLNERSVEYGGESGNGFQNERAVGYGGESGNGFLNERSVEYGGESGNGFQNERAVEYGGESGNGFLNERSVEYGGESGNGFQNERAVGYGGESGNGFLNERSVEYGGESGNSFLNKRAVEYGGESGNGFLNERAIEYGGESGNGFLNERAVEYGGESGNGFLNERAIEYGGESGNGFQNKRAVEYGGESGNGFLNERAVEYGGESGNGFLNERSVQNSGKSGNGFLNERSVQQGGKSGNGFLNERSVEYGGESGNSFLNKRAVEYGGESGNGFLNERAVEYGGESGNGFLNE

160

RSVQYGGESGNGFLNERSVQYGGESGNGFLNERSVEYGGESGNGFLNERSVEYGGESGNGFLNERSVEYGGEAGNGLK* Lottia Protein Model: jg i_Lotgi1_160696_fgenesh2_pg.C_sca_25000301 NR Annotation: No match found NR E value: Sequence: MKLLLLLTVLIPLCVSVPTKPGCCVPPVW EGIQGMFSGDGSGPAPELIQQSALISYDEKRQMININITQSIHGLTSDVRILTDYKKGVQYLINSKSRSCTTSKVPQTFQRFCIPDYFYYKGSFNVAFTLRASTEQFIVPLIWSKCKSNSSNFFKMRKACFSRDIQLVVGTCDCLFN* Lottia Protein Model: jg i_Lotgi1_163475_fgenesh2_pg.C_sca_40000029 NR Annotation: Myc homolog [Crassostrea virginica] NR E value: 8.00E-08 Sequence: MDLSYLYYGMFWFSCLLVSTVSAEFSYEEVTYRIPPTPTGFYDQNCTDKISDCEEFGIASCFKPYESWARENCAKTCEFCQGKPEKPPTCTDVEKGCDLYTTSICTDPSFISWAKAKCRRFCRMCPEQVLAELDLMTTTLTPVDCFDKVVCERYGKKACTGDFASWATDNCQKYCGFCSTGVTKSKRLCVDKLPNCPQYDSRICTDKKYYSWTDENCRSTCKFCQDKLVPFLIPTPQAVRTPKPPTT* Lottia Protein Model: jg i_Lotgi1_166720_fgenesh2_pg.C_sca_60000088 NR Annotation: No match found NR E value: Sequence: MMLLIKTGVLLIILFSLNTLCDSCACEFHPVLSGKWFFTGENKNINIKIHSKIIVFKVSNYLKLRYKCTERYGNIYLLRILCLFLEVDFLSDLFMKGGFTSIHVEGRGFDSFIFSPKNVTRKYANIDNTCDSEISRERKFIYRKGGCRVPRDMVGTW GFTFRQSSAITMGKRNLTIHFLNNDNLTLNCEARDRNMFLFRRSSYINSSTDAIICVQIDPVLDDPFYDYEFNRIITGNAVENLVRTVPRGKKLYINSDCDMIHSPARPEYIYTVDNIL* Lottia Protein Model: jg i_Lotgi1_166891_fgenesh2_pg.C_sca_62000015 NR Annotation: PREDICTED: similar to scavenger receptor class F, member 1 isoform 1 precursor [Canis familiaris] NR E value: 9.00E-05 Sequence: MATGLYFILSIVTCIINNCVIGLEICEDHYVSCPSQDCGIFPLEPLFRLHCMKTCGVCTDVNRASRKPTAQSSVDVNGMASHAVDGNTDLNICSKTNSSQPDTQWWCSDLQQVYEIDAIKIYSPVIGTKCNTGYYSQSSTSCSPCDTCSENSCEPTNGMCWDGCKQGFKGETCRQCEDGLFGPTCRFACNTTKCQSNCDRLTGICSECDTGYYGKNCELVCDKDCREGKCLKTDGSCTTDGKGSVVSTERAPVQHYTENINKYLVGVIGTFIGIVVTILLVLTYRCFKKRRSDTIYELPTRPPSKQELDFPLDPQYISSGVSTTSSRYTEVHPDSGSSHGSHYLARRNLSQYTHKLRPPAELPENEKRRLQAEMRQSKISGPRGKYMEYPNDAYDRDGSDYEAVKTEI* Lottia Protein Model: jgi_Lotgi1_171718_fgenesh2_pg.C_sca_119000006 NR Annotation: PREDICTED: similar to orphan G protein-coupled receptor HG38 [Gallus gallus] NR E value: 1.00E-15 Sequence: MAPIYLTFIASLLLASSTFCEQLCLEGCKCEDHPSEKLGPHLMIDCSNLGSSSVPRDLNKFQNYGRPMHLKLSKNIIARVSKADFPENLPIIALDFNQNRGVMITDDAFMNIKGLRYLGLNDISMPFTDTMKYLKGMTKLRYLYLNKNYQFGEGLVPVSLFSGLELKLKDLSLHDCYLKGIENGALANVVITGSLDIGANQLDHIPEEVKTLKNLTKLDVSQNSIKSIPSNTFEGMSKLRTLSLSNNYINGQSFKTDSFNGLKLKELRLEKSELNTIPSAALSSLSSLKILKLNNSPIKTIPKHSFNGSYCLEVLDLTNVLVEIENTHLDGLESCLQKLYLNNMQIREIPKFFKDFKVLNYVSLSGNSISEIKMDDLVGTTITELDLTENPLHRIEKGSFEEFPHAISLIISDTRLSNLSFVRDYEYGTIKTIKLGNVLP

161

MCDCELAFAEYRVLEIHGNCNFNNTKNVAISTDEFMSVTSHCWPDGKMPQKKDVWAVVGDNSAGTFSTSILSYFCVFLFFKVLNIV* Lottia Protein Model: jgi_Lotgi1_172278_fgenesh2_pg.C_sca_130000017 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO35011.1| p redicted protein [Nematostella vectensis] NR E value: 2.00E-26 Sequence: MGVKLLFLGICLIYLVLDLHDAEVTAVSDRQAKLKEKDAREKLQQELSKNYAVKQRRRKKKKFRDPRTRGKSSKVVNRGGAPKVQNGDDGGDDPHIGNRENYLQKYFKGKMW GIRDKKKAPKGNW RKKLRKQKHKKKEKRQNMKKKEKSKRTKVKKFKKKRKKHKKRRRGKRSTDENEDDPGSDQEHTHRTKRYAITDQERMW DYGVVPYSFSSDVTDVMARTYRGVFDALEKRTCISFVEW DGTESFYSNRSLNHMVHLNFVDQGGCWAYLGRYTYRTTNIAQDISCCYVGRTCLHEIMHSLSMEHEHTSPLRDGW LRVNW DNLNEDGQFQFSVIDPLMLGRESW GFDIKSFVSYGTTEFTKNGMMGFQPLMPDFYPTGNSYW QPFKEVQMNYDCFGIKCSGMEEPVCENDGYPGYYEGECGCVCPPGLDPATNCASIKPTDPIRGW PPSSFGLPEPIEGCPAGFQLGSLTQPLAGDSEVSSSSHINAGFENSTAEFGFCLKDTASQTEGGLTEWPRGQYCIHSYSNACPPGFQEGLITYDDSVNATRTILTESPLPDGSFVNNTELW YCCRSDGMVQLDIKLPNTESFILYRHGTAECQNVEGMYMFPEYYSLMIEGETIRSISVYQTLGSVPYNVLDGNELFVYYCYYSPLNKDCGEVIHLGPENPTHDIMTPNYPFDYPLNTECNW LFVAPEGSEVMLNFNDFDVEGDDFDCFDYVTIKRGLPGIPGVSYCGTEFKAAIKSIDRYLMITFTSGSEITYTGFHATGRLLDVADLCYNPDDFGRTYNGIHNYAEDFTECLPW VEMAHCDTNLFNTEDAFDNLDGNYCRNPGARYDSPWCYTEKENCTIKYCDVCQERTIYDNFDDCEELIAEHPNFCLDGDHHHYGCLATCIEYGHITIPEKSNRAQDVSCPVPDDIVDADPIVHDVTRYNVGDSLAYVCSNGTATKMRRCLTDGTWTDIEYVCGGCGDEMKSDTRGTCYAYVAEEVRFVEAEEYCIEKDWDVASAQTEDAFNFLVQYRSDMGSDGKQVW LDLYEDPVGVW SW GDESLVQDGFTNWRSTYPVSGPIETDGNNW YRCATMVGTGQSSVWNQVACDNKW YKAHYICQTMESEREICADRKSSCTRNLIDIPLLCSQYQSYAHEVCPYTCGICDVENADSCTVTADFVVGLNLVSGGSTPFSLNPGESFTTQCPAGQICTQNCQHFSRACRRSGEVTGATPVCQDADSVPTLVNNIERIPRLDTSEARRIYQGDNSYMNITRSGEITKWMTLCENDGIVHLMAFKYW SQDRYFKVMGVNQVECKAGRRMTW EIPEGERIQVDPSMNIGLMDWNGGCIPLYECFASEYPDLNKIYHTMAESQDEVALNKLVWMRAGPTCYFFSLNAEISPVGYIAPATTQLTTPSTTTEEPEPEPEATSTSSEDTSESTDMTTSSGDVSTSSTSTSAGNTESSSTGASRSSPSTDAFVSEDTTSSVW QRTTTHSVVITTPRRTINEDFIKSFIKKKKKKRKWWKNRGKKTDKTRKRKNKRRKKAQKGR* Lottia Protein Model: jgi_Lotgi1_172798_fgenesh2_pg.C_sca_142000011 NR Annotation: gb|AAA30047.1| calcium-b inding protein [St rongylocentrotus purpuratus] NR E value: 7.00E-07 Sequence: MRADSIQVRGLKALVLILLVLYEGHTKETCRTGVKTDKNLRGVNLDKTSGLPFYW FDLHRASDGYHSEYTYTFRLHSSSEASPFSDAVLQVETANNCGTGDLVVNTTFLYQPRNCPKMIMTEYKKPMTNVPFKWRAPKCGCIKFRATVIRNGVTYFYDDENISDGNLVKTVCPHGVRVAEQLVEVDEKTTDSTTKTLSTVTSKMDRAALLDLLCKITSTLNNLELLKRPEFIQRRKLQNSSLVKLLNIELALEQRKADISSCCHKKGEDKTICFDNVRRYRIDQLCGDGQPRIPFVEQKRNYMRKRESECCW RIGQPRYQCFSKSSEQLTEYSVDYSLDESDPANDIADYPKEVERYEMASNTKFFSPPPTLKPDNQNIETLTKLEETTTPTSVTKSVTLPPIRQPSARPHRQTTPTSVLTHHRQRPVPAVKTKLRQSRTVGQGDMGYNLRKQAKYTRKTTNLRHSASSVEITVNTLKQKLWKSSLKLECCQQGRIFAAKTGRTDVW DRCRSAAKDFRSSVVRGKKICSNTFQKCCVEQFITATAKASTIPKPNTPKGSPSTKQLVTTTSLRTNIKKENYRRAPLDVDSSREADEKYASMESKAEDDLMEQDDKASAEDAGEHVDDDGDDVSDDSDNDDQGEDGDQDEDDDQDDDDKDGDVDDDKVDEINNDEKNRKRKFIDYPREDPSADTFHNLQTKRKSTPNTDIAHRDSNHRNLKIESAKRKRNRRRHNRKLRRRKHKSD* Lottia Protein Model: jg i_Lotgi1_201804_estExt_Genewise1.C_sca_1950010 NR Annotation: DICTED: similar to putative porin precursor [St rongylocentrotus purpuratus] ref|XP_001192936.1| PREDICTED: similar to putative porin precursor [Strongylocentrotus purpuratus] NR E value: 8.00E-19 Sequence: MRLLIFTLSLLVAVVRSVDASQCPEPPSGFGPCVITDANCLTDSECSNGQICCPHLCGKICKDKVSLTVVKPG

162

TCPSNAAISCFVGSVADTCSSDSDCPGNQKCCNLCGKNCSPPQGSAPQLPPIGLPAPPPVCPPVCAIACEYGNVLDQNGCPTCSCKKTPCKGEAPLTLDRNGNTINCGRGGVRCPLNSECKIHPADAYALCCPIEPDDPKPGVCPKLPNPLPRCLIFQNDCKQDSECPGDQKCCSVTCGIGCIDPVKLCPVVDCLQYCEFGYRLDSNGCQTCQCKKSPCEDEAPMTLDSTGNTINCGRGGVRCPSNSECKIHPADRYAVCCPKVCPPVCEIYCEFGNVLDENGCKTCKCKKSPCEDGAPMTLDSNGNTINCGRGGVRCPLNSECKIHPADRYAVCCPKVCPPVCAIYCQYGNVLDENGCATCTCKKTPCKGEAPLTLDRNGNTINCGRGGVRCPLNSDCKIHPADAYAVCCPKEPSPVCKFGSCPVPPSSFLLRCASIDRCKYDSECEGTKKCCKHPCGRQCRDIVKSKPGVCPKKADPTLVLCKRRGDDCYEDATCPGSQKCCQGACGNACMDPRLTSTDHAGTCPALDPKIQSLILCASLDTECELDSDCKVDQKCCKDYCGIKCVKSVPKPIIKKGTCPRLRYRCRRKRRDFCRSDGDCPGDRKCCRLPCGYKCRRPKRIPIIRKECPAVNTHPIYKLFCSPPLNPPCQVDRDCPQGKICCPGVCANRCLKAPTKPVCDPNPCGDAQIKCQYGQTKDENGCLLCQCKSYDFKMS* Lottia Protein Model: jgi_Lotgi1_205030_estExt_fgenesh2_kg.C_sca_1120002 NR Annotation: PREDICTED: hypothetical protein [Strongylocentrotus purpuratus] ref|XP_001188672.1| PREDICTED: hypothetical protein [Strongylocentrotus purpuratus] NR E value: 3.00E-20 Sequence: MKLMFLGFIGILMLSTSNNASASPWFCKGLECPKFNLLNKTKDYEVRQYGSLTW VTTSHIGQWKSDILGELLFKNLFSYIEGNNTMKKQLDLAVPLLTKLESAGDRGSIYTMHLLIPRENIDHLPSPLNTNLTFITIPPSTYYIRSFDGVAYDRQYMKVLQELVNAINDTGAYPDDFFYTATYDHPQVKENRHNEVW LLKKEKIP* Lottia Protein Model: jgi_Lotgi1_150026_fgenesh2_kg.C_sca_22000005 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO30774.1| predicted protein [Nematostella vectensis] NR E value: 3.00E-25 Sequence: MLVYLLLAALLTDFSSAGVFYKDCGSVDGTISNIEVSSCASEPCQFQKNTNISVTIDFTSKTVVSSATTVVHGIIAGIPVPFTSVQPDACKDMSCPLASGTKYTYKNQVAVLPIYPTIQLVVQW EVKDQDGKDLLCFKIPVRITN* Lottia Protein Model: jgi_Lotgi1_166436_fgenesh2_pg.C_sca_58000065 NR Annotation: PREDICTED: similar to MEGF6 [Strongylocentrotus purpuratus] ref|XP_001187830.1| PREDICTED: similar to M EGF6 [Strongylocentrotus purpuratus] NR E value: 1.00E-26 Sequence: MMKILEILLCLSFFRATVQQFDCSVCKVCDNSTCIECIEGRYGISCGSFCSLKCSGRCRQSDGYCTSGCDEGYWGPGGLCDQTCSNCNPGGCVMNSGLCIAIGCRTGW YGTTCNNKCNSNCDGNCNQSNGNCNQCIPGKYGTDCQLDCSNCKDTCNKNNGVCNNGCKEGLW GTSCTKTCPNCQSSGCDIDNGNCNNPGCRIGW YGDSCNDKCNERCNDGCKQDTGYCNKCNKGVYGNVCDKNCNKGCLDGCNIDGSCKCKSTYYGTNCSKQCSSNCVDSLCNEISVGYNSCIRGCVAGYKGLYCDKQCPSSCPICNRFDGSCTECSKGFYGTECQLKCPEQCDKCKIKEGTCESCNPKYHGPVCSKRCSDKCLNGECNGVGGRCIDCIPGTFGDNCERSCSNGCIGDTSSTTCDRNGSCISGCINRIYGEKCNLNCSKNCYGSCNRSNGYCNSCLSGYFSDNCNQKCSIHCNGDCDRSTGNCGKCNEGYFGDNCNQNCSINCNGGCQKSSGSCLECNAGYYGDYCKQQCNDKCINNECLRNGQCRFGCMNGKTGSQCDGNCNSNCTICLQTDGNICKECKPGLYGISCDKLCNNTCKKVNSKPACNITTGKCTTGCIDGYKGYYCQDTCLDNCISCNNNQCTSCNTGW YGNQCQTKCNSNCINRCDQATGDCLDGCIDGKSGKHCKQEIG* Unknown Function

Lottia Protein Model: jgi_Lotgi1_105864_e_gw1.4.718.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO49128.1| p redicted protein [Nematostella vectensis] NR E value: 3.00E-15 Sequence:

163

ILTCLAFSLVWHSHLSGILTCLAFSLAWHSHLPGILTCLAFSLAWHSRLPGILTCLAFSLAWHSHLPGTLACLAFSLVWHSRLPGTLACLAFSLDWHSHLPGILTCLAFSLDWHSHLTGILSCLAFSLDWHSHLTGILTCLAFSLVW HSHLTGILT* Lottia Protein Model: jgi_Lotgi1_107224_e_gw1.6.194.1 NR Annotation: No match found NR E value: Sequence: CFLSVCLCSSICFTTECCFLSACLRVCFTTECCFLSVCLLFKRSRVCFTTECCFLSPCLRVCFTTECCFLSVCLLFKRSRVCFTTECCFLSVCL* Lottia Protein Model: jg i_Lotgi1_122612_e_gw1.41.141.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO49127.1| p redicted protein [Nematostella vectensis] NR E value: 1.00E-28 Sequence: MSYRRCFGISCSSMSFAVDWCILLVDGVAW CMLLVDVVDW CILLVDGVAW CMLLVDGVEW CILLVDVVDWCILLVDGVAW CMLLVDVVDW CILLVDGVAW CMLLVDGVEW CILMVDVVEW CILLVDVEDW CILLVDGVEW CILLVDGVEWCILMVDGVAW CMLLVDGVEW CILMVDVVEWSILLVDVEDW CILLVDGVEW CILLVDVVDW LILFGISRFKLELLHLEI* Lottia Protein Model: jg i_Lotgi1_132441_e_gw1.79.137.1 NR Annotation: PREDICTED: similar to CG11081-PA [Nasonia vitripennis] NR E value: 4.00E-72 Sequence: MAWKIYVLFVCVLCLRFILAARENYIKATFEGAKDRGIFNKLVVNRETNELYLAAVNHLYKLSPNLEVIQEVVTGPHLDDPSCTSLTNLTCDPDLKSCACKLRPYYTKALVVDYVNKKLITCSTLYHGHCDKLDLDNITNKQMFPFMPMVANNATASTVMFIAPGPAVVKDEPQRQVLYVAASYTRTGLKAYREQVPAFCSRSLEDFNLLLKNAFGSSSIEIESQHRDTFPVRYIYGFGSDNFSYVLSIQKANVQTDRYVTKISRICQSDDDYYSYAEVGLSCTMQNTEFNLLQAAYVGKSGTKLARSLGIPTTEDVLYTVFSIGDSNSANPSATSAMCVYSLRDIRKKFTENIQECFSGIGNTGPDHMIQSSKCLKVVSSHFFVIFFLESFESADLSVL* Lottia Protein Model: jg i_Lotgi1_143169_e_gw1.194.72.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO36917.1| p redicted protein [Nematostella vectensis] NR E value: 1.00E-28 Sequence: MVKVCSIPLTVVKVCSIPLTMVKVSSIPLTISKVCSIPLTMVKVSSIPLTMVKVCSIPLTMVKVCSIPLTIVKVSSIPLTIVKVCSIPLTMVKVSSIPLTIIKVCSIPLTVVKVCSIPLTVVKVCSIPLTMVKVSSIPLTMVKVCSIPLTVVKVSSIPLTMVKVCSIPLTVVKVCS Lottia Protein Model: jg i_Lotgi1_145599_e_gw1.360.11.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO49883.1| p redicted protein [Nematostella vectensis] NR E value: 6.00E-16 Sequence: LVVYCHCGLVVYCHW ELVVYCHW GLVVYCHW DLEVYCIVYCYW GLEVYCHW GLEVQCYW CLEVYCHCDQVVYCSW ELVVYCHW GLVVYCYW GLEVYCHW GLEVQCYW CLEVYCHCDQVVYCSW ELVVYCHWGLVVYCHW GLVVY*

164

Lottia Protein Model: jg i_Lotgi1_151060_fgenesh2_kg.C_sca_80000004 NR Annotation: LOC431836 protein [Xenopus laevis] NR E value: 1.00E-140 Sequence: MDFARLFQSLCICLLTLDIQLFGLTSGANPEVEQHLEMGKKLLAAGQLADALSHYHAAVEGDPDNYMTYFKRATVFLALGKSRSALPDLAKVVELRPDFTAARIQRGSVLLKQGKYKEAKNDFETVLKTSPNNEEAKRNIDKIETIPPDIELAYRLVEEGNYGDAIQVLGGIIDTCPWDPTFHEIRSECYEAQGDLFKAASDIRQSTKLRADNTRGYFKLSSLHYEMGEADESLVQIRECLKLDPDNKQCFPHYKKVKKLVKLMTSAQESINNEEYDKCVKKAEEMLKVESLVHHYVSRAGQHKCKCYSKGGKVKEALAECTHLLKIDPNNLDALIDRAEAHIFNEDYEAAINDYQEASNIDGENRRVKEGLNKANKLLKQSKKRDYYKILGVKRNAKKKAIMKAYRKLAVLW HPDKFTTDEEKEKAQKKFLDIAAAKEVLTDPEKRAQFDNGEDPLDPEEQSGHGGNPFQQGFNPFGQGFSGGSFKFHFN* Lottia Protein Model: jg i_Lotgi1_157590_fgenesh2_pg.C_sca_14000213 NR Annotation: No match found NR E value: Sequence: MLMFTSLLFVCFASIYAGESVYPDCQNPDNNERIPNGSKFKLENSGDCIEFTCKDGGYSASSVECDNNGECVAVGRTVEENCRSKKCVYDKFVGFQVTETKCEDDEGQCHSPGEEFTRTYQGTTYSKTTCTVDGNLVKYSYKN* Lottia Protein Model: jg i_Lotgi1_161950_fgenesh2_pg.C_sca_31000192 NR Annotation: RE49668p, putative [Brugia malayi] NR E value: 3.00E-08 Sequence: MKLGGDYGILCVLFTILTPVISIDCYACTSLGGNNEGCEDSFERTLKTVHFIARDCYYGYFRGTHCYKLKGERADGSSIMVRHCSDSHW GSHCGDIKYTNEHNVEEKIKGCLETCDHDGCNTATQKQFNFSLVIMAVLMTSALLL* Lottia Protein Model: jg i_Lotgi1_165259_fgenesh2_pg.C_sca_50000100 NR Annotation: hypothetical protein CHLREDRAFT_144736 [Chlamydomonas reinhardtii] gb|EDP05543.1| predicted protein [Chlamydomonas reinhardtii] NR E value: 6.00E-23 Sequence: MKVLLVLDICCLIGLVSVGEAVCPDNCNYNGVCDVKHSRCNCYEGFTGSDCKTDCRCNGHGTCQSGSVCKCDEGWKYSGGQCVW DCHCLNGAKCIGPGECGCVHNCKMGNCRNGQCQCW NGYKGSDCSEYDPTM* Lottia Protein Model: jg i_Lotgi1_166645_fgenesh2_pg.C_sca_60000013 NR Annotation: No match found NR E value: Sequence: MGASILLGLTILLSYVNQVHLQMFHPRDISRGGQQQEEQLRRGYLIQILKSFLNRRQEDATEKRKRSCNLNLGFHCQTDEYSSIADMYDFLQSALSPGKRKRNVKIVSIEGS* Lottia Protein Model: jg i_Lotgi1_167022_fgenesh2_pg.C_sca_63000026 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO43826.1| p redicted protein [Nematostella vectensis] NR E value: 2.00E-08 Sequence: MVAVRIRKYKNLLFFALLFAGVLSISTIFYSNHYQIMNTGTRSDVNTGFSYSNANSSKYVIYICDGKNSCGGYGDRQKGIVASYIISVMMNRQFGVIMNDSCDIKQMFKPNQINWIVDNNDIKSKTSKRIKALDGYSDKLRKS

165

MIEMDLDQEYKEEVIYFSLNLEFVFYLRQNKRYEKQLKW LEHLSMSEIYNVVWQSIFKLRPYIREKLDPFLDLKKQGLKLISAQIRLGKNPTIPHDSRVVNSLNNMNVLW QFYKKYNDSSKYRIFISTDSDTVREKARSIFPDVYSDVPGKVFHVERSNKTDICNGW RKVILDQVILSLSDVLVISNSGFGRIAAFFRQNENDLYCLNLDKIRRCTTQSEIFVDKTW* Lottia Protein Model: jg i_Lotgi1_168040_fgenesh2_pg.C_sca_71000102 NR Annotation: No match found NR E value: Sequence: MVGTRYLQLATSLAVFLLVLFLTCTQAAPVDDFETDNEALRKALFIARLLSSSDRLKNTKPEGSNLDFSELASIPFSNQQKRYRPPMQGRSGGMSLCLWKVCPAAPW LVSKRSEKTWDKNNMLGK* Lottia Protein Model: jg i_Lotgi1_170051_fgenesh2_pg.C_sca_92000069 NR Annotation: hypothetical protein TTHERM_00439050 [Tetrahymena thermophila SB210] gb|EAR97557.1| hypothetical protein TTHERM_00439050 [Tetrahymena thermophila SB210] NR E value: 3.00E-08 Sequence: MKMINIFIVVYCLISPCLGFW LSSKTVDPKVDISTECINKALTGDCGFFTCFEERLPCGEYGYAESYGGKYCWQFQQSHHLFTKKGVEFVEKLTRCHMNRSITSYRQNQIECFSHYDQSFVIMGDCYVESGFCDVVIDNFMSLARILEPKDFTNYRVVREVFRAAKQCPNNISGGIISKFLSYKRGSSL* Lottia Protein Model: jgi_Lotgi1_171167_fgenesh2_pg.C_sca_110000033 NR Annotation: No match found NR E value: Sequence: MAISHDWSFTLLW LIWIFTLLFSDSYGKRTQNYYMEGLDCGDSKHIGGATVYSNFRGDVSTVYGNDIECQMTFKAENKDWRLMLRIIELDIPDRTSTGLCNDALYVYDESSIYARAMEEANGNTGLCGNILPPTLYSTGQYLTVHFSVSLQNQKDIV* Lottia Protein Model: jgi_Lotgi1_171450_fgenesh2_pg.C_sca_115000014 NR Annotation: No match found NR E value: Sequence: MKRFTLLALLLVAILYISQSESKCQGVTKCMVNLFTCKREQISVNIAADCCKEYFECFNECKPGSRPPCKNTAPGKRGSWNKRYSSRYGSDILGDIISGY* Lottia Protein Model: jgi_Lotgi1_171757_fgenesh2_pg.C_sca_119000045 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO37253.1| p redicted protein [Nematostella vectensis] NR E value: 7.00E-24 Sequence: MWKIFSFHWIYFLFSLFCLLHSVTCVKKVSKTPRKSKESEVSKESTKRSASLDDDPSLWPGHLQPLGSSQQVHKVDAYKKYLKPEEFLNSYAAPYLPVLFQAVEQTSIAYEKW GNITYFTVLPQTLTNW VHISPEVGQVSFRKFLLNYTDKGSVFNNKVPFFMRSAKWEPYRDDVSVPKCLHCEPILNSFSTHKLW ISHTDQSSPIHRRNKDTIHCTFVGSQEFTLISHIEYGDKVPVDYTTIDINKVDFTKFPTLREVEYHKVRVKAGDCLYIPTKW YWQVRSTGKTYSADIEW YHRNKTVKSSNCKTKTSISSLNSVVFKDIDDPTTIETDVLLHYFTTYLLNVPKFTLPQFERHLKKDKKFMEDLIEWTDELQHITKEVFETLDVNKDSRFSMADLESLSQPMLEDIRGLFLDRLQDYDDILKDQQEEAKESKKQSEKENKAKDAPATTGPVDDYLKNYIGQLEDVLKESIEEMSVTGHLPDIKKKFAEKQAQGKTGNADKPKQSTKPKTEREKAKEKLEQDRQRRKEKQEAENIKEKTKDDEKYLIVDDSVEEEIMADDDDDSTTGSTNKRTPTADKADTKDNKSHTKDNKADTRHVDKKTGRKGQDGKEAKQKNTGSKIKTEL*

166

Lottia Protein Model: jgi_Lotgi1_173456_fgenesh2_pg.C_sca_159000027 NR Annotation: No match found NR E value: Sequence: MWSGVW CGVVYGVMWSGVW CGVWSGVERSVVW RGVWSDVDGVMWKECGVVYGVVW CMKWCGVWSDVERSVVWCMKWCGVW SDVERSVVW CMEW CGVWSGV Lottia Protein Model: jgi_Lotgi1_173484_fgenesh2_pg.C_sca_161000006 NR Annotation: No match found NR E value: Sequence: MRLHYLVTVLVVLILIDVGEGQDNISKCLELPSVKEIVTRYICPKPDPCATVDCNCNGKCVDGKCQCKNGFFGEFCTALTSLIPKVAGFVSNNKSLISSEAQTIGSIAKAGVSKKSKELKELQQIRKLRNRRLEEKKGKDFKDIQKSLGSLLGFPWAKYPGEKHLQDHNYTGPGTRLDLRLDENDKPKPGGNETFTFDFISIMLGKKQFE* Lottia Protein Model: jgi_Lotgi1_175543_fgenesh2_pg.C_sca_302000002 NR Annotation: No match found NR E value: Sequence: MNTMFLLVVFLLIPATSAFDSTQTNRTIVNSTITTKINGSSIGLGTCSEFTPQSCSGVCGQFFELW CSCDEYCQVYRRCCFDYEAVCKEESEKSKQDHENLMNVTVECDSNMSGVHVPQQRISPASG* Lottia Protein Model: jgi_Lotgi1_176704_fgenesh2_pg.C_sca_2718000001 NR Annotation: PREDICTED: similar to rCG46800 [Nasonia vitripennis] NR E value: 6.00E-09 Sequence: MLTIRVLGVFAVIVLAFVLVESDVELCEDDVNVCHGELPANAMCSGENNKTKRCQCKKGYKMSLDIDCTSNGSVFSCQDIDECATNNGSCEYKCQNEPGSYNCSCPEGSELQGNGLNCGPSGP Lottia Protein Model: jg i_Lotgi1_203811_estExt_fgenesh2_kg.C_sca_240005 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO37140.1| p redicted protein [Nematostella vectensis] NR E value: 0 Sequence: MMKLFVILALLAGQSLADIYLHNPRGSNDRLNEKSAQRANANRLFDSQNNNRGGYNVGDVTSAPHGKDASKQYKMAYFQSEADAETILTVEW YNQHGCGGNEDDNPQKQNCRLVLQYMCQPEGTTEDVLRNGVVTNTQDYNRPPNSNYNLANRNSRKNNNVKADRGLQESW DW YEECFVRERNKGLFTADQNLRGNNGLGYSSAIYTRQNPNGNRRGYECPEERDYFPYW HPTQWKDIAILAENETLCNPLIAESFNNKPYGACLQKFPNSQVRKPSRYNNKEECEENGLDW VAFHNYLEKDKTASTQAACQAKSTNNIEYTWAIPYDSTTFTAECLVKVPSPQCQEAPWSRSNHLGNGIGGEMNTVQWTLPHFPSGQVQNCVMRMRYNISTDDYDPFNTDSEFNGADNSPVTNNPYVDIGVGRGPLRLAINTAQFGRVFQDRTHIFQLKPRPDDIKDFRIFNVNVRGKRGNIVQVYPAVEYDFTPTDLEVTENDLVHFQWTGSNTHNNGRPGGDGQTGDAGEGKSGSDRHNILQMADRNENFPLPFEKTNMWDNAEIKWIYHGKNDVSARDLALNMASSGYYKCVSATDCPAESFKDFLVDTKDKMQVTLDNTPASYPGVVLKFKQGKYHYMCTRNNNFTNRSQKATITVAA* Lottia Protein Model: jgi_Lotgi1_204607_estExt_fgenesh2_kg.C_sca_690020 NR Annotation: ctg4a [Aedes aegypti] gb|EAT44181.1| ctg4a [Aedes aegypti] NR E value: 2.00E-45 Sequence: MAFMFFQTLLIIVSIGYLHADDSDGVQEATLCEVCRILAEELQLRLDETGKSKEVIETGHGLDTKKKKKYNF

167

SELRLIEALQEPNICDRILEYSVHKEKTGIERFAKGRSQTMEALHGLVNKGVKVELGMPHEMWDKPSVEITQMQRKCYALVEEYDEDIEDW YYNHQDQPFLDYFCRNLVLNPQNNECLDEDMRSQNEGSSSEMKGDKGKKEEL* Lottia Protein Model: jgi_Lotgi1_204654_estExt_fgenesh2_kg.C_sca_720011 NR Annotation: PREDICTED: hypothetical protein [Monodelphis domestica] NR E value: 8.00E-93 Sequence: MSLLIATGLFCVIYLLVKFLNISQPCHLPELYFKDKTSNFIQTVFTMCPILFESYIPPLLW GKSGHLQTFIYAKMGRIMSPLPNGKRIELIRPDNATMSFDVFQPHYEHPTGRDYTLAVCPGIANSSESLYIRTLVDHAQYNGFRVAVLNHLGALRKVKLTSPRIFNYGETGEYNCMIDELKRLYPNTSIIAVGCSMGANIVIKYLGECKQHENKVIGALSFCQGYDVNDAKPFLLGW ESCRRLYCYFMAINLRQLLRSHQDILFTEQAQSEYGTVELKKLYSATSLLAIDELYSVNAFKFKCCEEYYHWASCKQYIQNITKPIFILNAEDDPIVPKQLYTTPLKYQENHDNCLFIVTKHGGHLGFFEGGFIKPNTITW LDRAVVQFSNVLCELDCKERKS* Lottia Protein Model: jg i_Lotgi1_67930_gw1.39.124.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO32094.1| predicted protein [Nematostella vectensis] NR E value: 1.00E-10 Sequence: VGHFVLSCCMW RAVFSCCMWRVVFSCCMWRAVFSCCMWRAVFSCCMW RAIFSCCMW RAVFSCCMWRAVFSCCMWRAVFSCCMWRAVFSCCMW RAVFSCCMWQHVASCIQLLHVASCIQLLHVASCTQLKHVASCIQLLHVASCTQLKHVASCIQLLHVASCIQLLHVASCI Lottia Protein Model: jgi_Lotgi1_98330_gw1.10.599.1 NR Annotation: hypothetical protein NEMVEDRAFT_v1g147894 [Nematostella vectensis] gb|EDO28414.1| predicted protein [Nematostella vectensis] NR E value: 5.00E-06 Sequence: TVCSLGTHCLSITVCSLGTHCLSITVCSLGTHCLSITVFCSLGTHCLSITVCSLGTHCLSITVCSLGTH CLSITVCSLGTHC Lottia Protein Model: jgi_Lotgi1_154212_fgenesh2_pg.C_sca_5000252 NR Annotation: pred icted protein [Monosiga brevicollis MX1] NR E Value: 2.00E-97 Sequence: MNLKLYILICVTVSGILGTPLDDYVNRPDSTYKYFEITHYVHEDHTLYILNMTSQTW LTAEISTQPIWWHYLTVTIPHKIRYPDAAFMFIEGGSNHNGPPTGTDNFVALTTAMAVSTGTIGADLKMIPNQPIMFKADPTKKQRTEDAIIAWTWKTFVETNGSNPDLLLRMPMTKASVRALDTIADFAMDKVGNKVDKFFIAGASKRGWTTWTTAAVDNRVVAMAPIVMDLLHMVKNLHHHYRAYGGWTFAFKDYYSLNFTQDLDSPWTQAMADIIDPISYNDRYANIPKLVVSTGGDEFFLPDDSHFYFKELMGPKYLRILPNAEHSCAGHEISLLFTMRAFYLSVITKTPLPQLTW VREETPSGGRITVTCNQKPKTVNVFYARTLDGNRRDFRLLVGTPGDPSKPMPHPVVW LRDDVQDMGNNVYRAELTKPEIGW TAFFIQLTFQGFADSDLEFTTETQIIPDIFPFPDCTGQECKGTLV* Lottia Protein Model: jgi_Lotgi1_160458_fgenesh2_pg.C_sca_25000063 NR Annotation: predicted protein [Monosiga brevicollis MX1] NR E Value: 6.00E-86 Ac Annotation: no hit Sequence: MNYKMILNKYTLVLGIILVCTQPCITDVNITKVHLIFMNHLDVGFNGITPQTGFVINVINKYFTKYFPGAIELALQMNFLGYRERLIYTTHPW LVSLYLDCPQNLTMSGIQLMCPTEEQREGFLYAAKRGDITW HAGPMNMQYEALDTAMVNFSLKLSEDMDARMDIKRRHRVLSQRDVPGMTRALIPIFNNHGIEGVTVGVNSVSSPPAVPK

168

IFKW VFQNSSVIAMWHPGGYPIKPGSFPSMPLGLSRDNCVTFEQFDEAMCFSFRGDNQGPPTSIAEVLNSYEIVRGQFPGAIVEGSAFENFVEVVQSVKDKLPVVDQEIGDTWIQGISSDPGKTAMMRGFFRARTACLTSAPNFEDVISSWSEQRQFIDLALETLGDHPLVQAVEEEYKQLVPQAPDLSKYKQLSATTFDCQNGFKFSFAADGSLNKLQDKDGRNWASKSQPIGQLLYKTYNDTDFDSFQKQYVLNHYYW FQVGKWNLTANCPTCESTIWNTKLQKLYAASDGSCDVVALLAMEEDRATSFYGAPPKIYVKYLSSSNTFLSIEVQLFGKKPTRLAESLSLSFQPIRQEGSKW YLHKLGQLIDPLNVVTNGSQRLHAVDEGVQYKDSNGKGMMIGSKDVGLATVHQTPTDVSVLPVPLTPIQSISGVSYNIFNNVWDCNYIFW YPFLKQDSNSKYRFSITFS* Lottia Protein Model: jgi_Lotgi1_161657_fgenesh2_pg.C_sca_30000077 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO44809.1| p redicted protein [Nematostella vectensis] NR E Value: 3.00E-17 Sequence: MTKLSEYFLICSMIFVVFMLQRGTCDDVVTEEQISYAKGSVCGYCSYCKFCKLCDKDCPCETSPSKPNCKMCKYCKYCYICSGVCDTICQPGGMIDKISAAIVNALPSFNKEEIEDDLESVKPW LDKKDEL* Lottia Protein Model: jgi_Lotgi1_164905_fgenesh2_pg.C_sca_48000037 NR Annotation: no hit Sequence: MMTKMLTIIFSVLIVLCSSSPECGPNYFEGDKWFDGCNW CTCTDNKEKCTNATCTEYQDELDNSPCQKLGESWRDGCYHCRCLPDGIRCRANKRCVHALKSDDLNCPVDGLCVCGLDGVPSCNEQIMSRFSFKNVPELGPDTSSDKKLILSSKGENTLRLNPDEAETDLGDKDLHLIPTDDKLCHLGPRW YGGGLRCYCDPQNGVACGNPRFIARKVDGFKIRSKKCRVGKIWEERCRICTCKKSGLSLCRIDRECTKNKNIRHSVKSIVVAGNAECRVGLRW KDGCKRCRCNKRGKIICKTRLCNLSDDGKKLSNKDKTHRQTSTGGLISKSTPRFQLPEKPRFRMPNLSLYNNRFQRKKRPSIKTVNCNGFKPGEKYWDKCNLCHCTMAGAPMCSSKLC* Lottia Protein Model: jgi_Lotgi1_166736_fgenesh2_pg.C_sca_60000104 NR Annotation: hypothetical protein LOC500991 [Rattus norvegicus] sp|Q5XI89|FA55D_RAT Protein FAM55D precursor gb|AAH83798.1| Hypothetical protein LOC500991 [Rattus norvegicus] NR E Value: 3.00E-25 Sequence: MALTKKLVLICCLIIATVFGLKIMHTIKYQINMRPNIEPIPFYSQNTPKRKLMFPERQSPSLEDYNMKHDDVRSEDIFNKFLSENVVYVKDTTADDYFIEGEKELLEEGEIKDLRDLPSSNYSIISTKNTVFNQYEAVTVKIQLYNGFGQLLKRGGDDVRVW ITGENFQAGGEVYDLHNGTYVAEVKAFWTGKSKIHVSIAYSREELRILLRTRRESKVLRYSVGRFLRGGDTFMTLCSPFHDIPLYPRVCNFTNFNLNPYYCGQPNSPKVACSDLRILHTINYSKQRMNNLESKILSRQNRVNELKSKSITINIGTVVNLPYCSSYKSRFTWTNTAPLGYFNNNTWHPSFCQGQITRQRLSRCIRDLHVYLVGDSTVRQIFQSFLRLTKCTNTTFIGNRPHDRVQRYCDVHDSNFRVEYGIHGLPYSMVRTPDLLNNTKAIGFHLDGLQWSQNKDKMFVMVHLYAHFLFFHTSLFEARVRSLKVPLTRLLNRYPKIKIFIKGPHAFTGADFKPIVKSDYFGKVYRSILLKELFEFRDRVYYLDYWDMSVSSGNRLLHPVNSFVDEMVKFFLNLACDK* Lottia Protein Model: jgi_Lotgi1_168278_fgenesh2_pg.C_sca_73000118 NR Annotation: no hit Sequence: MSISASSYSGTFKLCLFIVVLVAFSSYCGFVITREDSQFSNHLPKLTSTNVILEKLDSLRKDFLKQRTNVAGQFKNLGSKLSELVLNKNTKPENQHIKAQDEPWRKSTKTGLTLFTSW VDVPEKDLVHGNVVRMW RKWKPLIKPLFFYSSASTGKRLEKEGW LVQPVSKTGCGVTEMPTIKDMFLDAMKNHESVLYGYANGDITFDDGMPKAIDYVVNMDVVREKPVLVLVRRTNVDFSNGPELDANSDITEMYNEGKALKDGSSDGFFTNKLFPWKYLPDIVIGRIGIGMW LASYARAMNVTVIDITKTVKAIHMTTKSGNSESHFKKNGRCNHVIYGRLKLSPTSW GCGFITCATIDITFDKQGQTTMRRKNPKSMNPKCGNCSMDLTQILPKSLGITDRIYFGQNIEPKQVVNKRPKSPK* Lottia Protein Model: jgi_Lotgi1_173484_fgenesh2_pg.C_sca_161000006

169

NR Annotation: no hit Sequence: MRLHYLVTVLVVLILIDVGEGQDNISKCLELPSVKEIVTRYICPKPDPCATVDCNCNGKCVDGKCQCKNGFFGEFCTALTSLIPKVAGFVSNNKSLISSEAQTIGSIAKAGVSKKSKELKELQQIRKLRNRRLEEKKGKDFKDIQKSLGSLLGFPWAKYPGEKHLQDHNYTGPGTRLDLRLDENDKPKPGGNETFTFDFISIMLGKKQFE* Lottia Protein Model: jgi_Lotgi1_176362_fgenesh2_pg.C_sca_1085000001 NR Annotation: no hit Sequence: MVAKGTVGILIVLFNTICANIDLNYNQVPVANPLLFGRRGINPDMSSLFFGKRSGNSDHRDLRKMKDTCKAVLSSCKILFSDYEDDTVRNKVQDGFGRFK* Lottia Protein Model: jgi_Lotgi1_177026_fgenesh2_pg.C_sca_12012000001 NR Annotation: no hit Sequence: MMTKMLTIIFSVLIVLCSSSPECGPNYFEGDKWFDGCNW CTCTDNKEKCTNATCTEYQDELDNSPCQKLGESWRDGCYHCRCLPDGIRCRANKRCVHALKSDDLNCPVDGLCVCGLDGVPSCNEQIMSRFSFKNVPELGPDTSSDKKLILSSKGENTLRLNPDEAETDLGDKDLHLIPTDDKLCHLGPRW YGGGLRCYCDPQNGVACGNPRFIARKVDGFKIRSKKCRVXKIWEERCRICTCKKSGLSLCRIDRECTKNKNIRHSVKSIV Lottia Protein Model: jgi_Lotgi1_202874_estExt_Genewise1.C_sca_21760001 NR Annotation: pred icted protein [Nematostella vectensis] gb|EDO37140.1| predicted protein [Nematostella vectensis] NR E value: 2.00E-54 Sequence: MMKLFVILALLAGQSLADIYLHNPRGSNDRLNEKSAQRANANRLFDSQNNNRGGYNVGDVTSAPHGKDASKQYKMAYFQSEADAETILTVEW YNQHGCGGNEDDNPQKQNCRLVLQYMCQPEGTTEDVLRNGVVTNTQDYNRPPNSNYNLANRNSRKNNNVKADRGLQESW DW YEECFVRERNKGLFTADQNLRGNNGLGYSSAIYTR Lottia Protein Model: jgi_Lotgi1_202874_estExt_Genewise1.C_sca_21760001 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO37140.1| predicted protein [Nematostella vectensis] NR E value: 2.00E-54 Sequence: MMKLFVILALLAGQSLADIYLHNPRGSNDRLNEKSAQRANANRLFDSQNNNRGGYNVGDVTSAPHGKDASKQYKMA YFQSEADAETILTVEW YNQHGCGGNEDDNPQKQNCRLVLQYMCQPEGTTEDVLRNGVVTNTQDYNRPPNSNYNLANRNSRKNNNVKADRGLQESW DW YEECFVRERNKGLFTADQNLRGNNGLGYSSAIYTRQNPNGNRRGYECPEERDYFPYW HPTQWKDIAILAENETLCNPLIAESFNNKPYGACLQKFPNSQVRKPSRYNNKEECEENGLDW VAFHNYLEKDKTASTQAACQAKSTNNIEYTWAIPYDSTTFTAECLVKVPSPQCQEAPWSRSNHLGNGIGGEMNTVQWTLPHFPSGQVQNCVMRMRYNISTDDYDPFNTDSEFNGADNSPVTNNPYVDIGVGRGPLRLAINTAQFGRVFQDRTHIFQLKPRPDDIKDFRIFNVNVRGKRGNIVQVYPAVEYDFTPTDLEVTENDLVHFQWTGSNTHNNGRPGGDGQTGDAGEGKSGSDRHNILQMADRNENFPLPFEKTNMWDNAEIKWIYHGKNDVSARDLALNMASSGYYKCVSATDCPAESFKDFLVDTKDKMQVTLDNTPASYPGVVLKFKQGKYHYMCTRNNNFTNRSQKATITVAA*

170

Object 2-6. Predicted signaling molecules found in Lymnaea stagnalis.

Likely to be functional secreted signaling molecule

Lottia Protein Model: jg i_Lotgi1_113861_e_gw1.18.363.1 NR Annotation: PREDICTED: similar to tartan CG11280-PA [Apis mellifera] NR E value: 5.00E-27 Sequence: MVKPPTQNTRWWIIVWILLFQKYTCEPEVTCPHGCKCDVDVVNCLHSNLTSVPVGLSNGDRLQELKLGRNKITEIKLELLEYKELRLLDLISNNISTIDGSAFRTLYKLETLFLSRNFITKTDKNTFRNLTSLRFLDLSYNNLRCLKIGNFEDLQSLVNLDLSRNQISLIEAESFRCLENLEILKLPRNQLDQFPW KIFQNLTSLKMLVLDDNLLTSLPISAFSNLINLEYLSLTGNRLHELLPSSFMMSDEGAISLEQLYLRGTDLDTIPVKLFAKLKKLTVLDLSKTQIVSVKNENLNGLSSLESFIMTSSNNLTYIEKGVFQDLHSLRKIDISGNTLLSEIQFTTSSLRNLSLFDLSANSL Lottia Protein Model: jg i_Lotgi1_157019_fgenesh2_pg.C_sca_12000330 NR Annotation: No match found NR E value: Comment: Similar to Chordin Sequence: MFSLVLLVVTPWLVFCMPPPPPAMCTCSDGTQVPPGGTHSISDCHSCYCDENNSEAQEIIAACLIVPCVDSVKMPGQCCPTCPNGENCRAEFKDDNGVERSEIIKVSDGVVKFNSTECQCDYQNYNGEPKANCVQVLSIEPVIAEKSTP* Lottia Protein Model: jg i_Lotgi1_159314_fgenesh2_pg.C_sca_20000054 NR Annotation: Buccalin p recursor [Contains: Buccalin-D; Buccalin-E; Buccalin -F; Buccalin-G; Buccalin-H; Buccalin-A; Buccalin-I; Buccalin-J; Buccalin-K; Buccalin-L; Buccalin-B (BUCb); Buccalin-M; Buccalin gene-predicted acidic peptide A (BGPAP A); Buccalin-N; Buccalin-O; Buccalin -P; Buccalin-Q; Buccalin-R; Buccalin-C; Buccalin-S; Buccalin gene-predicted acid ic peptide B (BGPAP B)] gb|AAB27696.2| buccalin precursor [Aplysia californica] NR E value: 3.00E-66 Sequence: MAARKHELVLVLTSVLCFVSSIVGDPNVPSDSQDNSALTQDDFAKRGMDKFGFAGGVGKRGLDKFGFTGQLGKRDMDSFGFAGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFAGQLGKRGLDQYGFTGQLGKRGLDQYGFAGQLGKRGLDHYGFAGQLGKRGLDQYGFAGQLGKRGFDQFGFAGQLGKRGLDHYGFAGQLGKRGLDQLGFTGQLGKRQMDIFGYRGQLGKRQSIDKYSFLGAGIGKRSVKNTAGIKKDDA* Lottia Protein Model: jg i_Lotgi1_164016_fgenesh2_pg.C_sca_43000014 NR Annotation: chordin [Danio rerio] sp|O57472|CHRD_BRARE Chordin precursor (Protein chordino) gb|AAB93485.1| chordin [Danio rerio] NR E value: 1.00E-119 Sequence: MSPMDLAVCLVLLAATVSSTKFSKIPLKAARENLFGIQPNRPGCILGSKFYGI GERWTPELLPVGEMYCVKCECVPVERKGIIDMKGQTLCKNIKNLCPRPNCLNPVLPKGKCCKVCPDEVNSFEDSFSLGSKSSAPTMISSDSKRRVRNEFVSLLVGKNVRRESVKTSAVAAVYFSIVGRDEIRYSVRYFKLDRPKFLQITDANGNVLFERPIEKKRNGDKKFCGIWTKVPSAYIQYVREERLFAVITASRHFRGLVSGRIMVNRHAKREVFSSVLASPKANGIGGLLSVVYNPRTHLVDYVINLDGVFPGSSEEYFITISKKSKVLHQSKGKASSKTRFIRGSWTLKNKRQSKQLARGRLTIRLTSTNGASVSGDIKPKVTCGVSQAVMSSGNSLEEMKLASSGTAVFELREKGSLDYKIRVTGLTSKVHRIRLEGAVNKKNRRQIIGSVHRNFKADENSFNGW CNGTFKKMTADDLHLFLNNKIFVNVATSENRISELRG

171

RVIALPYHSHFEQVSADPAALGPKTITSNGHAGNAWISMGVGCSLHYDITTTTIAKHHDLTLSLVSPTDIDAYVTIEKISTTNLYEFQQASGSIAVVPESLFRDLDEGSALIQVISGDVILSGNVSVPNTCWQYSQDYDMDILVDEQTSEEVLAANRYKCVYEGKVYENGDSW LPDVNATCNTCSCNKGKIECHRLICPVVECANPIVLDGECCPTCPIETTDQTECKIDGDLRSHPVGSTWHPFLPLMGYAKCVICTCLPGGIYKCERKCPKLDCPASRRIRLNPKDCCQVCAPEEVKPEVVKPLQDVDMKGACSFKDQIYANGAKWHPRVIPFGYMKCIDCSCNNGTPSCSRPKCPKLSCSRKIREDGACCDTCADSDGEEIVEEKKSDSCIFGGKKYDDGETFQPKTSSLSSCATCTCRKGSMKCSLTCPEKCKDPKHSNDPCCKHC* Lottia Protein Model: jg i_Lotgi1_164520_fgenesh2_pg.C_sca_45000185 NR Annotation: PREDICTED: similar to filaggrin 2 [Rattus norvegicus] (molecular regulat ion of epidermal terminal differentiat ion) NR E value: 5.00E-37 Sequence: MIKLTLSILDIVQIVKVTFSILDTVQIVKLTLSILDIVQMIKLTLSILDIVQIVKVTFSILDIVQIVKLTLSILDTVQIVKLTLSILDIVQIVKLTLSILDTVQIVKLTLSILDTVQIIKLTLSILDIVQIVKLTLSILDTVQIVKLTLSILDTVQIVKLTLSILDTVQIVKLTLSILDTVQIVKVTFSILDTVQIVKLTLSILDTVQIVKLTLSILDTVQIVKLTFSIFDTVQIVKLTLSILDIVQIVKVTFSILDTVQIVKLTLSILDIVQIVKLTFSIFDTVQIVKLTLSILDIVQIVKLTLSIHVVC* Lottia Protein Model: jg i_Lotgi1_167943_fgenesh2_pg.C_sca_71000005 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO34356.1| p redicted protein [Nematostella vectensis] (Fulicin-like) NR E value: 1.00E-21 Sequence: MKSILVTSVVVVAGTLLTLVQALGADKFAEPATQKHSKIPTAALSHSGDTLLNTDAHQTNPSNIWKSKSKFDLDALPVVNRQDVQSHYAQHRRSYDTPMFLGKRDLKNLSLRFKRATDLEDLDATEFSDDFDKRGVDIPQFIGRRRYDTPQFIGRRGYGVPAFVGKRAFRAPAFIGKKAFGAPAFIGKKAFDAPSFVGKRKLTAPSFIGKRGLDVPAFVGRRAYAPPQFIGRRGMDVPAFVGKREFQTPMFLGKRAFSAPSFVGKRGLDAPTFIGKRKEESFRDLLATLQAYREYRRLMQTDKRFDAPLFVGKRSDYENDNLAAETLSRRFQCIPQW VFRMRLNVVDLNMSFIGGYKQNYFSLNILDDVQTKILLPLDDLHKVDSSKMDVPTVQLWDADMPRPNGARFLKLDPAHALGFLGQEASPVSKRYLEFVGKRDNDKRYAEFVGKRAGFGEKRYSRGSLSEYLSKRLRIRRPEFVGKRSNQKRYLEFIGR* Lottia Protein Model: jgi_Lotgi1_168508_fgenesh2_pg.C_sca_76000035 NR Annotation: ependymin-related protein precursor; EpenHg precursor [Holothuria glaberrima] NR E value: 3.00E-10 Sequence: MMKYCLVVAVVVVGVVIGEPSKPGCCTPSQWEGQQGSTIGIVDGGIPSVRTSMVRISYDEKNQRIAYVTSESTPQGEKNYKIIYDYSRKFQFIVDLDTKHCTVSSLTDPFMKLCLPVDAQPKGKFYYGIDGNNLPAMAFSLNVNGTMITVVTTADNCIPIAEVLVDGQAQGVSQMHASGFVNLTSGIKDPSVFIPPGTCTQ Lottia Protein Model: jgi_Lotgi1_169325_fgenesh2_pg.C_sca_84000054 NR Annotation: Insulin precursor [Contains: Insulin B chain; Insulin B chain'; Insulin A chain] gb|AAF80383.1|AF160192_1 insulin precursor [Aplysia californica] NR E value: 4.00E-13 Sequence: MEVTCKCPLVLLGVLFLNFGTVLTHLEWTCTLETKRESPRGVCGQRLPEVLSMVCKRYGGYRDTWFRKRNGEGTNSRLGNIILGKRDAFSYLGKRGQSYGEQGITCECCYHSCSFRELRQYCRNSQQRISIKK* Lottia Protein Model: jgi_Lotgi1_179503_fgenesh2_pm.C_sca_71000002 NR Annotation: Wnt7 [Euprymna scolopes]

172

NR E value: 1.00E-122 Sequence: MTVSPTVVWAAFSMALSSVVALGANIICNKIPGMAPKQRAICRSRPDAIVSIGEGAKLGLTECQYQFRFMRWNCSTLDSNDSMFGYESLGGTKEAAFIYAMTSAGVSYAITQSCGLGSLPNCGCDKDKGDGKLAPQGWKWGGCSADIKHGLRLARKFMDAREIAQNARSLMNLHNNRAGRKAVKDNMGTDCKCHGVSGSCTMKTCWTTLPPFRKIGDSLKKRYKKSKIVVPYLGRRARTAVTLILKRAKRPHRKPRRSHLVYLDKSPNYCDFDGKTGSLGTVGRKCNRTTKDTDGCDLMCCGRGYNTHQYTRTWQCNCKFHW CCYVNCNKCSERTEEYTCK* Lottia Protein Model: jgi_Lotgi1_188920_estExt_Genewise1.C_sca_260301 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO37057.1| p redicted protein [Nematostella vectensis] (growth factor) NR E value: 1.00E-109 Sequence: MFLEVFNISATVLCILFLIGHRKCDTIQNSKHHAELMMDLLQPYKNYHGQRLKKSNRHIRECQEIKYGNVTHSKVKVKPDTNISYPLVEVKHLTKEIGSYYYKNPVTIRYQRVDFPLNTLSVLEPGEPGTCREGHGTRSITSKTALSGNCIVGVNGGFFNTTTGACLGNIVSNGRLVQDSQGLQNAHFGITEDGYIYVGYLSELDLITQQFKQLLGGVLW LIRDGEKYIDKSKQMECPDVEETGTIETFIEVQSARSAVGHDKDGRVIMVQVDGKTNARGVNLHEFTDILLELGVVNAINLDGGGSTTTVINHTLVNYPSDKCADGTFSCEREISTILCVHEPECQPNDCNQHGHCVLGKCVCDGYW VGSDCSSLSCPFSCSHHGTCTKDGCICDDGW YGINCSTPCKPGYYGSNCAHQCYCLNHGTCNSQTGHCVCSPGFTGKYCESLCEFGYYGDKCNNLCTCDNGCFCHHVTGSCNLS Lottia Protein Model: jgi_Lotgi1_208417_estExt_Genewise1Plus.C_sca_10710 NR Annotation: fasciclin- like protein NR E value: 5e-178 Sequence: MSRNLWIFLLILLSIIVIASSRRSRGRW YRGRSGRRSLADRLRDRGTKPSNHRPRQRHGW GRW GSGW GAGQDHRTRVEEELDDLFRRIKDRRYGFNFEDKFW LDFGWNSRNEPKPWWEGPNVCKDEKESIENTSISDTGPISRHFSFKNQVCDQSETSYKCTTKTGIFMKQKTVTEVYECCPGFTRKDEMFGCPTEFKLDGLVKTARNLGLTEFLRATESVSLTDDLEKGNFTVFAPVNKAFEDEKNILPELETILPKDMPSVVMVSKPLTDLVIADMSNVLLGHLVYDTLKTSGLNDEQLIETASPFDSLLRVNFFSRPEKLITVNCARILSADNQATNGIIHTVDRIIKPVTDSIVDIISQNPNLSYLKTALARADLVKALRGTGQYTLFAPTDAAFEKLEPSIRDRLIKGEKSCLEKVLKNHLLTSVICSTVIQGTVTTTNLLNKYLNLTRTEDDKLFVEGSQMVGRDIMATNGVIHVIEDVLVPDQALGLIDVAEKNNLTKFVELLKETGMFKGITKMENLTLFIPSNNAFEKLEEKVSLEELKKDKKKVEDIVMYHVVPEVVTCSRLHNNYKLSTENERNIRMNRYSTFPFRHHSIQTAQCAPIIKENVPACNGVINIVNEVLLPPVGNVVDNLASKKKFSTLVRLMKKSGLADMLQEEGPFTMFAPTNEAFDDLYEEDLEKIEDNPEVLKKVLQNHIVQEFVCCASISMVRSWFDVASVRTLSGVRFPVKERHGTIQFNHAVVTECDNTATNGVVHAIDKVTLSPERPFWKRDFYHW* Predicted molecules found in Lymnaea stagnalis with unknown function or predicted to be

related to the secretory pathway

Lottia Protein Model: jgi_Lotgi1_103075_e_gw1.1.1164.1 NR Annotation: PREDICTED: similar to CG31650-PC, isoform C [Apis mellifera] NR E value: 9.00E-50 Sequence: MNVYYNILILCLGVYCTLGEKEKVPNEPVDQKHTKDGEHNPHYDHEAVIGSKQLEHEFDELPPAEAIQRLENLVKHHDLNADNIITHDELKKW ILASYMSLDEEESDEKFQENDVDGNGNVSW RELVKKEFGYDLEDIEDFKKNTDKQEPDTKEFLLMLDEEEKRFMTADTDNNGNLEKQEFRAYYHPNEYPHMHGVEIERALRTHDKNKDNQVSKAEFIGGLLFLEDREVYLTEEENFNSFDLDGNGKLSQDEVRAW VIQDNNQAAEDEVLHLISQADDNKNNELTLAEILEHYDDFVGSATDYGHKLSDEL*

173

Lottia Protein Model: jgi_Lotgi1_105864_e_gw1.4.718.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO49128.1| p redicted protein [Nematostella vectensis] NR E value: 3.00E-15 Sequence: ILTCLAFSLVWHSHLSGILTCLAFSLAWHSHLPGILTCLAFSLAWHSRLPGILTCLAFSLAWHSHLPGTLACLAFSLVWHSRLPGTLACLAFSLDWHSHLPGILTCLAFSLDWHSHLTGILSCLAFSLDWHSHLTGILTCLAFSLVW HSHLTGILT* Lottia Protein Model: jgi_Lotgi1_107224_e_gw1.6.194.1 NR Annotation: No match found NR E value: Sequence: CFLSVCLCSSICFTTECCFLSACLRVCFTTECCFLSVCLLFKRSRVCFTTECCFLSPCLRVCFTTECCFLSVCLLFKRSRVCFTTECCFLSVCL* Lottia Protein Model: jgi_Lotgi1_108564_e_gw1.8.40.1 NR Annotation: PREDICTED: hypothetical protein [St rongylocentrotus purpuratus] ref|XP_001179636.1| PREDICTED: hypothetical protein [Strongylocentrotus purpuratus] NR E value: 2.00E-86 Sequence: MYLSCYLPVILLFIEDLKQAVVAMMLRKDEVEEKNKSLKAMLDREMEISSTLRAEIEEMKISFKLSKDKEIAKNETLQKENELLKHQLRKYINAVQLLRTEGAKDDTQGITLEDPQPIIPPAKPSIDYSHEASEYEKKLIQVAEMHGELMEFNELLHRQINCKEAVIRNLKEELTDLRGPLPYDAQSSDDSLSGDLESSLISRCLINIWIPSAFLGGSKTDSHHVYQVYVRIRDEEW NVYRRYSKFLDVHTRLKKVYPLIEKFEFPPKKTIGSKDPKVVTARRKMLQSYLRKVINHLLEKNADLSSNVSKEKLIAVLPFFK* Lottia Protein Model: jg i_Lotgi1_116422_e_gw1.24.410.1 NR Annotation: PREDICTED: similar to CG14764-PA [Apis mellifera] NR E value: 7.00E-58 Sequence: MKTKNFYILVLLISVTSVALW LVSYPSPTFRNIMSTTQNNIRKIPKHMQMSKSPDLEVDKKYLDLLGFDSESKPKNDIVVIGSALMPADTENTILFLKSAQEYVPNLKLVLFDMGLSKADKQTLTNCRNETKHCEIRKFNFDQYPSHISDFANKSYKPICIQLLLKEFGTVIWADTSELFASSDIDNTMKQAKTEGLVAWTIEDATSSLTHPKMFDYFKTDQKKYFFHHAVQTSHIILVNTDKIHENVMLPW VKCALVEECINPMGAQNSGCNYQRKPKFRYTGCHWYDMSALNVILGLAFDFDEAYSGKDKIFWTKFDQEKAKAENMTDGAVHKVSVPHRVYF* Lottia Protein Model: jg i_Lotgi1_118077_e_gw1.28.72.1 NR Annotation: LOC100124858 protein [Xenopus tropicalis] NR E value: 1.00E-108 Sequence: MGSTLNILVILGCILVPFSFSNFLEW GPDLEGPW CATRPIEQCCPGRDDECTVPILGTKCYCDIFCNETAEDCCPDFW NLCLGVTRPTPWPLTTTTSRVPINILCNVYW FKCTKLHFSSHWECSNDDCLLEADHITQINNGPYSWVASNYSDFW GLTLEDGVKYRLGTFPLGSNVVQMTPLRVKLTDVLPESFDARTKWPSYIKPIRDQGNCGASWAFSTTAVASDRLAIESLGEIKDELSAQHMLSCNVRKQKGCEGGNLDRAWWFLRKTGVVTEECYPYES GDTTDKGDCLVSRRKSNTTCPSRILYKEKRRYKATPPYRIAPKEREIMKEIMDNGPVQATFMVKSDFFMYKNGVYRYSNISFDEEPAVYHSVRIIGW GVERTIYGDILKYWICANSW GTQW GENGYFRIIRGRDECEIESYIVGVW GQISGDASLRTLLTRGRRRRLFAERSLRNLRVNSMAKGRKSRSRQVGRKKVKRGRKSREERRRLRQEHRNKRKLRRQSRKQDSDSQNTV*

174

Lottia Protein Model: jgi_Lotgi1_118302_e_gw1.28.365.1 NR Annotation: No match found NR E value: Sequence: CVMLYMLCKCSSLDCVKLYVLCECSICHCVKLYVLCECSICHCVKLYVLCECSICHCVKLYVLCECSICHCVKLYCSICHCVKLYVICECSICHCVKLYMFCECNICHCVKLYVLCEV* Lottia Protein Model: jg i_Lotgi1_122612_e_gw1.41.141.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO49127.1| p redicted protein [Nematostella vectensis] NR E value: 1.00E-28 Sequence: MSYRRCFGISCSSMSFAVDWCILLVDGVAW CMLLVDVVDW CILLVDGVAW CMLLVDGVEW CILLVDVVDWCILLVDGVAW CMLLVDVVDW CILLVDGVAW CMLLVDGVEW CILMVDVVEW CILLVDVEDW CILLVDGVEW CILLVDGVEWCILMVDGVAW CMLLVDGVEW CILMVDVVEWSILLVDVEDW CILLVDGVEW CILLVDVVDW LILFGISRFKLELLHLEI* Lottia Protein Model: jg i_Lotgi1_143169_e_gw1.194.72.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO36917.1| p redicted protein [Nematostella vectensis] NR E value: 1.00E-28 Sequence: MVKVCSIPLTVVKVCSIPLTMVKVSSIPLTISKVCSIPLTMVKVSSIPLTMVKVCSIPLTMVKVCSIPLTIVKVSSIPLTIVKVCSIPLTMVKVSSIPLTIIKVCSIPLTVVKVCSIPLTVVKVCSIPLTMVKVSSIPLTMVKVCSIPLTVVKVSSIPLTMVKVCSIPLTVVKVCS Lottia Protein Model: jg i_Lotgi1_145599_e_gw1.360.11.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO49883.1| p redicted protein [Nematostella vectensis] NR E value: 6.00E-16 Sequence: LVVYCHCGLVVYCHW ELVVYCHW GLVVYCHW DLEVYCIVYCYW GLEVYCHW GLEVQCYW CLEVYCHCDQVVYCSW ELVVYCHW GLVVYCYW GLEVYCHW GLEVQCYW CLEVYCHCDQVVYCSW ELVVYCHWGLVVYCHW GLVVY* Lottia Protein Model: jg i_Lotgi1_151060_fgenesh2_kg.C_sca_80000004 NR Annotation: LOC431836 protein [Xenopus laevis] NR E value: 1.00E-140 Sequence: MDFARLFQSLCICLLTLDIQLFGLTSGANPEVEQHLEMGKKLLAAGQLADALSHYHAAVEGDPDNYMTYFKRATVFLALGKSRSALPDLAKVVELRPDFTAARIQRGSVLLKQGKYKEAKNDFETVLKTSPNNEEAKRNIDKIETIPPDIELAYRLVEEGNYGDAIQVLGGIIDTCPWDPTFHEIRSECYEAQGDLFKAASDIRQSTKLRADNTRGYFKLSSLHYEMGEADESLVQIRECLKLDPDNKQCFPHYKKVKKLVKLMTSAQESINNEEYDKCVKKAEEMLKVESLVHHYVSRAGQHKCKCYSKGGKVKEALAECTHLLKIDPNNLDALIDRAEAHIFNEDYEAAINDYQEASNIDGENRRVKEGLNKANKLLKQSKKRDYYKILGVKRNAKKKAIMKAYRKLAVLW HPDKFTTDEEKEKAQKKFLDIAAAKEVLTDPEKRAQFDNGEDPLDPEEQSGHGGNPFQQGFNPFGQGFSGGSFKFHFN* Lottia Protein Model: jg i_Lotgi1_152847_fgenesh2_pg.C_sca_2000267 NR Annotation: SARP-19 precursor [Littorina littorea] NR E value: 5.00E-08

175

Ac Annotation: CNSN01-F-148317-501 Ac E Value: 2.00E-07 Sequence: MIAFICVLGFISGCLAAPCTHTNSAEHTEEQVIARVIALADHNNDGILSVTDLAAYFVINFDHNHNSEVDKNEFILQWHETFHDEKAFAEHVFTHFDTNADNVLNLSDLFALNIRLDTDGNGTVTNKELESYLQLIYNAC* Lottia Protein Model: jgi_Lotgi1_154212_fgenesh2_pg.C_sca_5000252 NR Annotation: pred icted protein [Monosiga brevicollis MX1] NR E Value: 2.00E-97 Sequence: MNLKLYILICVTVSGILGTPLDDYVNRPDSTYKYFEITHYVHEDHTLYILNMTSQTW LTAEISTQPIWWHYLTVTIPHKIRYPDAAFMFIEGGSNHNGPPTGTDNFVALTTAMAVSTGTIGADLKMIPNQPIMFKADPTKKQRTEDAIIAWTWKTFVETNGSNPDLLLRMPMTKASVRALDTIADFAMDKVGNKVDKFFIAGASKRGWTTWTTAAVDNRVVAMAPIVMDLLHMVKNLHHHYRAYGGWTFAFKDYYSLNFTQDLDSPWTQAMADIIDPISYNDRYANIPKLVVSTGGDEFFLPDDSHFYFKELMGPKYLRILPNAEHSCAGHEISLLFTMRAFYLSVITKTPLPQLTW VREETPSGGRITVTCNQKPKTVNVFYARTLDGNRRDFRLLVGTPGDPSKPMPHPVVW LRDDVQDMGNNVYRAELTKPEIGW TAFFIQLTFQGFADSDLEFTTETQIIPDIFPFPDCTGQECKGTLV* Lottia Protein Model: jg i_Lotgi1_157590_fgenesh2_pg.C_sca_14000213 NR Annotation: No match found NR E value: Sequence: MLMFTSLLFVCFASIYAGESVYPDCQNPDNNERIPNGSKFKLENSGDCIEFTCKDGGYSASSVECDNNGECVAVGRTVEENCRSKKCVYDKFVGFQVTETKCEDDEGQCHSPGEEFTRTYQGTTYSKTTCTVDGNLVKYSYKN* Lottia Protein Model: jg i_Lotgi1_159188_fgenesh2_pg.C_sca_19000225 NR Annotation: predicted protein [ Nematostella vectensis] gb|EDO44886.1| predicted protein [Nematostella vectensis] NR E value: 2e-07 Sequence: MRGPTWSVILLLINLIVTLQACDDVTEFQCADNNQCISKTDTCNTINDCDDGSDENNCACLEKLGMEDGTILDSSLIASSSKTGSSPSYARASNDKGNW CPKSDDSKPFFKVKFDTPRAISAIVFTLKQSVVDPLNYLSTWTLKAVLPGSDEEVTLYEGLNAFSNNSLYRSLFFPSLVTDTFIIAPTTW QNEPCLSFELYGCNIDNLCPTGCQNNGKCSGNDICACADGYFGTKCQFQKTTIPFSSYIFKSLESGSLVTSSETFTVRTSTTIRTSKIIEIQGRKVLSLPVADYFTAPKDGKFGCLSKLGSGGCKSFYLALGCELEELKDTVQLFTTGSDSTYIGAEATLKSDNLVVTFRTSELIWTLNVRKTNMFSSSIKYVLEFDWDISTGIRIYVNKKLIGQQRQATRTTQVLRTNTLATSGVEFFKSKSVNVKLFSLKSSSVNRISLIKTSLISLTPTEECDQEKEHKCANSDKCIDKSNVCDSVEDCDDGSDEQNCPTTTCDVKVYKKLVTKSASLSLDLLEVDDAQRVAKCTLPVISYVVDGILLDFLSKNQYGDQLSATNDLGANQNHNNNNKDDNNNNKKSSQDEFKEDVFNWADEPWTPCDDVEVPDWARMDDPTFDNFNSELIWAEDPWTQLPLVGLPSWMLLPGIDYPVEAKDPVVYTPVNDEDDAAFLASGIDIVNWSDEPYFQLPLDVPRWALLPGIDYPVRHGYNSPYWTYKLNQYNSSRREFGKKMFARQFPTLREERVLYNNNGQSKLCIPGNFQRRNHRF* Lottia Protein Model: jgi_Lotgi1_161657_fgenesh2_pg.C_sca_30000077 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO44809.1| p redicted protein [Nematostella vectensis] NR E Value: 3.00E-17 Sequence: MTKLSEYFLICSMIFVVFMLQRGTCDDVVTEEQISYAKGSVCGYCSYCKFCKLCDKDCPCETSPSKPNCKMCKYCKYCYICSGVCDTICQPGGMIDKISAAIVNALPSFNKEEIEDDLESVKPW LDKKDEL*

176

Lottia Protein Model: jg i_Lotgi1_163300_fgenesh2_pg.C_sca_39000034 NR Annotation: PREDICTED: similar to TTC17 protein, partial [Bos taurus] NR E value: 1.00E-133 Sequence: MASSVVVIVLLHFLFEQCLLTRGSMHW VVTEDGRIQSQADSVYNLRRPYDLVAFMHQEDRATVLNHLKKELLNRKGEIDKSEDRDTTGLEQKFYKSDYDCLQAGTPLPEIDLYISTILPIEHKHISDELSIEIQKAAQSSDNKIGTIPPEPDCTKVLQLDFTPHGFEHLEGVQERMNLTGSAELGLTHAIPFIPDKTEIPDFGHVIAKELKKNSTSWVLYNMAAFYW RVKGDPWMTVECLRRALHFSPRENKDIALISLGNILHRARYSNEAAIVVHSALELSKELNVNHFTLGNIYAVLGEYNKSIICYENTLRIQPDFEAATIRKHAVLCHQKLEAALEAQHRSLQKTLRDLKDYQKKHDLWHIQNDRMSVEQVSQEEKVSQNIAFEFNKAKQTSTKDIGEYCNMVEREGKQVLLCTWNRQAPTLEMLDQFALEEQKKESERNRLKLNKYEKKAIDYNLPVRAPLYVKHDRQAVPRFNELELDNTWPEKEECDSHLLTSPDPFNLSTVYLSPENKGFEVKALLTEAQNLKDGAEHPLPW YPPICVTLLTINEYDEKTYDNLKSVGHPGRTKVPLKMYDPSMRKTLLSHVNGGTVTEEEVGQRILSAIKQEVGARW VLFNLAGLYW RIVGNNYHGIECIRRSLALAPDEYADVPLVNMANILYKW GRYDDAIVLMKDALKINDAEPDSNFLMANLLW VTKNYSGAISHYRTVLDIIPEHQEAFDSLRAIKCLQKFLIKEQSVAPIEVPSQPVGNSCQQKGVANKNQQTESRVICKTENGEEKCIIETRMRGKSGECNGHCTQTCTITPIKVESSCGGPDLAVDTSNPVQCHQKGSQCGEAGEELLFTNDFTNKLDEVSDHYEKKGICNGEDCNTLRVQCLIPMKTHSGLVAHVITPPKLFVRPLSLHSTQCMTEQKKPHIKLEFIDGVLHQKLIFVQDANDIHVEDNECIIFNDGIKSPGCDQAQYRSYSEELARSNINVEFRYVGDESPVEKDKKNHCSKTPNSPGVPSDEIPYSDISDGILQSLNGDVEPSEVVGHRIALTLQQNPKSW VAASAAGLYW RVEGDFTKALDCYRLSLSYAPLEMADLMLLGYANILVQGGYYSNAIMVEDLALDIQSTLPIHYFTLGNIYAALGKWRTAVSFYEATRFYQEDFKPAVDRLRAIHCENIA* Lottia Protein Model: jgi_Lotgi1_166436_fgenesh2_pg.C_sca_58000065 NR Annotation: PREDICTED: similar to MEGF6 [Strongylocentrotus purpuratus] ref|XP_001187830.1| PREDICTED: similar to M EGF6 [Strongylocentrotus purpuratus] NR E value: 1.00E-26 Sequence: MMKILEILLCLSFFRATVQQFDCSVCKVCDNSTCIECIEGRYGISCGSFCSLKCSGRCRQSDGYCTSGCDEGYWGPGGLCDQTCSNCNPGGCVMNSGLCIAIGCRTGW YGTTCNNKCNSNCDGNCNQSNGNCNQCIPGKYGTDCQLDCSNCKDTCNKNNGVCNNGCKEGLW GTSCTKTCPNCQSSGCDIDNGNCNNPGCRIGW YGDSCNDKCNERCNDGCKQDTGYCNKCNKGVYGNVCDKNCNKGCLDGCNIDGSCKCKSTYYGTNCSKQCSSNCVDSLCNEISVGYNSCIRGCVAGYKGLYCDKQCPSSCPICNRFDGSCTECSKGFYGTECQLKCPEQCDKCKIKEGTCESCNPKYHGPVCSKRCSDKCLNGECNGVGGRCIDCIPGTFGDNCERSCSNGCIGDTSSTTCDRNGSCISGCINRIYGEKCNLNCSKNCYGSCNRSNGYCNSCLSGYFSDNCNQKCSIHCNGDCDRSTGNCGKCNEGYFGDNCNQNCSINCNGGCQKSSGSCLECNAGYYGDYCKQQCNDKCINNECLRNGQCRFGCMNGKTGSQCDGNCNSNCTICLQTDGNICKECKPGLYGISCDKLCNNTCKKVNSKPACNITTGKCTTGCIDGYKGYYCQDTCLDNCISCNNNQCTSCNTGW YGNQCQTKCNSNCINRCDQATGDCLDGCIDGKSGKHCKQEIG* Lottia Protein Model: jg i_Lotgi1_166645_fgenesh2_pg.C_sca_60000013 NR Annotation: No match found NR E value: Sequence: MGASILLGLTILLSYVNQVHLQMFHPRDISRGGQQQEEQLRRGYLIQILKSFLNRRQEDATEKRKRSCNLNLGFHCQTDEYSSIADMYDFLQSALSPGKRKRNVKIVSIEGS* Lottia Protein Model: jg i_Lotgi1_166720_fgenesh2_pg.C_sca_60000088 NR Annotation: No match found NR E value: Sequence: MMLLIKTGVLLIILFSLNTLCDSCACEFHPVLSGKWFFTGENKNINIKIHSKIIVFKVSNYLKLRYKCTERYGNIYLLRILCLFLEVDFLSDLFMKGGFTSIHVEGRGFDSFIFSPKNVTRKYANIDNTCDSEISRERKFIYRKGGC

177

RVPRDMVGTW GFTFRQSSAITMGKRNLTIHFLNNDNLTLNCEARDRNMFLFRRSSYINSSTDAIICVQIDPVLDDPFYDYEFNRIITGNAVENLVRTVPRGKKLYINSDCDMIHSPARPEYIYTVDNIL* Lottia Protein Model: jg i_Lotgi1_166891_fgenesh2_pg.C_sca_62000015 NR Annotation: PREDICTED: similar to scavenger receptor class F, member 1 isoform 1 precursor [Canis familiaris] NR E value: 9.00E-05 Sequence: MATGLYFILSIVTCIINNCVIGLEICEDHYVSCPSQDCGIFPLEPLFRLHCMKTCGVCTDVNRASRKPTAQSSVDVNGMASHAVDGNTDLNICSKTNSSQPDTQWWCSDLQQVYEIDAIKIYSPVIGTKCNTGYYSQSSTSCSPCDTCSENSCEPTNGMCWDGCKQGFKGETCRQCEDGLFGPTCRFACNTTKCQSNCDRLTGICSECDTGYYGKNCELVCDKDCREGKCLKTDGSCTTDGKGSVVSTERAPVQHYTENINKYLVGVIGTFIGIVVTILLVLTYRCFKKRRSDTIYELPTRPPSKQELDFPLDPQYISSGVSTTSSRYTEVHPDSGSSHGSHYLARRNLSQYTHKLRPPAELPENEKRRLQAEMRQSKISGPRGKYMEYPNDAYDRDGSDYEAVKTEI* Lottia Protein Model: jg i_Lotgi1_168040_fgenesh2_pg.C_sca_71000102 NR Annotation: No match found NR E value: Sequence: MVGTRYLQLATSLAVFLLVLFLTCTQAAPVDDFETDNEALRKALFIARLLSSSDRLKNTKPEGSNLDFSELASIPFSNQQKRYRPPMQGRSGGMSLCLWKVCPAAPW LVSKRSEKTWDKNNMLGK* Lottia Protein Model: jg i_Lotgi1_170051_fgenesh2_pg.C_sca_92000069 NR Annotation: hypothetical protein TTHERM_00439050 [Tetrahymena thermophila SB210] gb|EAR97557.1| hypothetical protein TTHERM_00439050 [Tetrahymena thermophila SB210] NR E value: 3.00E-08 Sequence: MKMINIFIVVYCLISPCLGFW LSSKTVDPKVDISTECINKALTGDCGFFTCFEERLPCGEYGYAESYGGKYCWQFQQSHHLFTKKGVEFVEKLTRCHMNRSITSYRQNQIECFSHYDQSFVIMGDCYVESGFCDVVIDNFMSLARILEPKDFTNYRVVREVFRAAKQCPNNISGGIISKFLSYKRGSSL* Lottia Protein Model: jgi_Lotgi1_171718_fgenesh2_pg.C_sca_119000006 NR Annotation: PREDICTED: similar to orphan G protein-coupled receptor HG38 [Gallus gallus] NR E value: 1.00E-15 Sequence: MAPIYLTFIASLLLASSTFCEQLCLEGCKCEDHPSEKLGPHLMIDCSNLGSSSVPRDLNKFQNYGRPMHLKLSKNIIARVSKADFPENLPIIALDFNQNRGVMITDDAFMNIKGLRYLGLNDISMPFTDTMKYLKGMTKLRYLYLNKNYQFGEGLVPVSLFSGLELKLKDLSLHDCYLKGIENGALANVVITGSLDIGANQLDHIPEEVKTLKNLTKLDVSQNSIKSIPSNTFEGMSKLRTLSLSNNYINGQSFKTDSFNGLKLKELRLEKSELNTIPSAALSSLSSLKILKLNNSPIKTIPKHSFNGSYCLEVLDLTNVLVEIENTHLDGLESCLQKLYLNNMQIREIPKFFKDFKVLNYVSLSGNSISEIKMDDLVGTTITELDLTENPLHRIEKGSFEEFPHAISLIISDTRLSNLSFVRDYEYGTIKTIKLGNVLPMCDCELAFAEYRVLEIHGNCNFNNTKNVAISTDEFMSVTSHCWPDGKMPQKKDVWAVVGDNSAGTFSTSILSYFCVFLFFKVLNIV* Lottia Protein Model: jgi_Lotgi1_171757_fgenesh2_pg.C_sca_119000045 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO37253.1| p redicted protein [Nematostella vectensis] NR E value: 7.00E-24 Sequence: MWKIFSFHWIYFLFSLFCLLHSVTCVKKVSKTPRKSKESEVSKESTKRSASLDDDPSLWPGHLQPLGSSQQVHKVDAYKKYLKPEEFLNSYAAPYLPVLFQAVEQTSIAYEKW GNITYFTVLPQTLTNW VHISPEVGQVSFRK

178

FLLNYTDKGSVFNNKVPFFMRSAKWEPYRDDVSVPKCLHCEPILNSFSTHKLW ISHTDQSSPIHRRNKDTIHCTFVGSQEFTLISHIEYGDKVPVDYTTIDINKVDFTKFPTLREVEYHKVRVKAGDCLYIPTKW YWQVRSTGKTYSADIEW YHRNKTVKSSNCKTKTSISSLNSVVFKDIDDPTTIETDVLLHYFTTYLLNVPKFTLPQFERHLKKDKKFMEDLIEWTDELQHITKEVFETLDVNKDSRFSMADLESLSQPMLEDIRGLFLDRLQDYDDILKDQQEEAKESKKQSEKENKAKDAPATTGPVDDYLKNYIGQLEDVLKESIEEMSVTGHLPDIKKKFAEKQAQGKTGNADKPKQSTKPKTEREKAKEKLEQDRQRRKEKQEAENIKEKTKDDEKYLIVDDSVEEEIMADDDDDSTTGSTNKRTPTADKADTKDNKSHTKDNKADTRHVDKKTGRKGQDGKEAKQKNTGSKIKTEL* Lottia Protein Model: jgi_Lotgi1_172278_fgenesh2_pg.C_sca_130000017 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO35011.1| p redicted protein [Nematostella vectensis] NR E value: 2.00E-26 Sequence: MGVKLLFLGICLIYLVLDLHDAEVTAVSDRQAKLKEKDAREKLQQELSKNYAVKQRRRKKKKFRDPRTRGKSSKVVNRGGAPKVQNGDDGGDDPHIGNRENYLQKYFKGKMW GIRDKKKAPKGNW RKKLRKQKHKKKEKRQNMKKKEKSKRTKVKKFKKKRKKHKKRRRGKRSTDENEDDPGSDQEHTHRTKRYAITDQERMW DYGVVPYSFSSDVTDVMARTYRGVFDALEKRTCISFVEW DGTESFYSNRSLNHMVHLNFVDQGGCWAYLGRYTYRTTNIAQDISCCYVGRTCLHEIMHSLSMEHEHTSPLRDGW LRVNW DNLNEDGQFQFSVIDPLMLGRESW GFDIKSFVSYGTTEFTKNGMMGFQPLMPDFYPTGNSYW QPFKEVQMNYDCFGIKCSGMEEPVCENDGYPGYYEGECGCVCPPGLDPATNCASIKPTDPIRGW PPSSFGLPEPIEGCPAGFQLGSLTQPLAGDSEVSSSSHINAGFENSTAEFGFCLKDTASQTEGGLTEWPRGQYCIHSYSNACPPGFQEGLITYDDSVNATRTILTESPLPDGSFVNNTELW YCCRSDGMVQLDIKLPNTESFILYRHGTAECQNVEGMYMFPEYYSLMIEGETIRSISVYQTLGSVPYNVLDGNELFVYYCYYSPLNKDCGEVIHLGPENPTHDIMTPNYPFDYPLNTECNW LFVAPEGSEVMLNFNDFDVEGDDFDCFDYVTIKRGLPGIPGVSYCGTEFKAAIKSIDRYLMITFTSGSEITYTGFHATGRLLDVADLCYNPDDFGRTYNGIHNYAEDFTECLPW VEMAHCDTNLFNTEDAFDNLDGNYCRNPGARYDSPWCYTEKENCTIKYCDVCQERTIYDNFDDCEELIAEHPNFCLDGDHHHYGCLATCIEYGHITIPEKSNRAQDVSCPVPDDIVDADPIVHDVTRYNVGDSLAYVCSNGTATKMRRCLTDGTWTDIEYVCGGCGDEMKSDTRGTCYAYVAEEVRFVEAEEYCIEKDWDVASAQTEDAFNFLVQYRSDMGSDGKQVW LDLYEDPVGVW SW GDESLVQDGFTNWRSTYPVSGPIETDGNNW YRCATMVGTGQSSVWNQVACDNKW YKAHYICQTMESEREICADRKSSCTRNLIDIPLLCSQYQSYAHEVCPYTCGICDVENADSCTVTADFVVGLNLVSGGSTPFSLNPGESFTTQCPAGQICTQNCQHFSRACRRSGEVTGATPVCQDADSVPTLVNNIERIPRLDTSEARRIYQGDNSYMNITRSGEITKWMTLCENDGIVHLMAFKYW SQDRYFKVMGVNQVECKAGRRMTW EIPEGERIQVDPSMNIGLMDWNGGCIPLYECFASEYPDLNKIYHTMAESQDEVALNKLVWMRAGPTCYFFSLNAEISPVGYIAPATTQLTTPSTTTEEPEPEPEATSTSSEDTSESTDMTTSSGDVSTSSTSTSAGNTESSSTGASRSSPSTDAFVSEDTTSSVW QRTTTHSVVITTPRRTINEDFIKSFIKKKKKKRKWWKNRGKKTDKTRKRKNKRRKKAQKGR* Lottia Protein Model: jgi_Lotgi1_176362_fgenesh2_pg.C_sca_1085000001 NR Annotation: no hit Sequence: MVAKGTVGILIVLFNTICANIDLNYNQVPVANPLLFGRRGINPDMSSLFFGKRSGNSDHRDLRKMKDTCKAVLSSCKILFSDYEDDTVRNKVQDGFGRFK* Lottia Protein Model: jgi_Lotgi1_176704_fgenesh2_pg.C_sca_2718000001 NR Annotation: PREDICTED: similar to rCG46800 [Nasonia vitripennis] NR E value: 6.00E-09 Sequence: MLTIRVLGVFAVIVLAFVLVESDVELCEDDVNVCHGELPANAMCSGENNKTKRCQCKKGYKMSLDIDCTSNGSVFSCQDIDECATNNGSCEYKCQNEPGSYNCSCPEGSELQGNGLNCGPSGP Lottia Protein Model: jg i_Lotgi1_192261_estExt_Genewise1.C_sca_450209 NR Annotation: conserved hypothetical protein [Aedes aegypti] gb|EAT46740.1| conserved hypothetical protein [Aedes aegypti]

179

NR E value: 1.00E-128 Sequence: MTLYITSLVFFLFINGTLQVKNRSSKVADRCFCKLNGEIDDCSCKVETLSVLNNNKIYPRIKSLLSRNYFRYFKVNLKKKCPFWHDDSRCALKDCHVDTCKEEEIPSAIKKGSSAYRYSEEAQKEESCAEEKELSALNTTISDESIQAFKDW KEHDESQDLFCDIDDESSADSEYVDLLLNPERYTGYKGESPHRIW RSIYQENCFKPQTEYIYGPSKSSSPLCLEKRAFHRLISGLHTSINIHLCADYLYQENLGYGMKAHWKPNVAEFQKRFDPQTSNGEGPQRLKNLFFTYLVELRAIAKAAPYLQEEDFFTADSDEDKDVRQGIDDLLNIISTFPDHFDESKLFAGNPNEAKLLKDEFRNHFRNVSRIMDCVGCDKCKLW GKLQTQGMGTALKILFSGDDIGPDSTVNAQQKKHFQLTRSEIVTLFNAFGRLSKSIHSLEMFKSLLSKS* Lottia Protein Model: jgi_Lotgi1_197084_estExt_Genewise1.C_sca_820083 NR Annotation: glucose-regulated protein 94 [Crassostre NR E value: 0 Sequence: MKRFIVLGILGFLFIAGAGLLNVQAEEVVTEPDVVEDIGKSREASRTDDEAVQREEEAIKLDGLNVAQMKELREKAEKFAFEAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRFVSLTDKSAMAATDELSIKIKADKASHVIQITDTGIGMTKDDLVKNLGTIAKSGTSDFLSKLGESKDQAQMSDLIGQFGVGFYSAFLVADRIVVTSKHNDDDQYVW ESDSSSFSVAKDPRGNTLGRGTMITLHLKEEAHDYLEESTIRSLVKKYSQFINFDIFLWASKTEEVEEPIEEEEEEKKEEEKKEDEDAEVEEEKEEKPKTKKVSKTIWDW ELINNVKPIWTRKPSEITDDEYNDFYKSISSDYEKPMTKIHFTAEGEVTFKSILYVPNSSPSDTFQNYGKQMEQIKMYVRRVFITDDFEDMMPKYLSFIRGVVDSDDLPLNVSRETLQQHKLLKVIKKKLVRKTLDMIKKIDKDTYEKFW KEFSTNIKLGVIEDTTNRTRLAKLLRFSSSNSDTELTSLAEYLERMKEKQEHIYFVAGHNRAEVEKSPFVERLLKKGYEVLYLTEPVDEYCIQSLPEFESKKFQNVAKEGLKLDDSEKAKERKEATEKEFEPLITWLKDSALNDKIEKATISERLASSPCALVASQYGWSGNMERIMQAQAYAKAKDPSQEFYATQKKTLEINPRHPLIKELKKRVEANKEDETAKDLAVVMFETATVRSGYRITDTAGFAQRVENMLRLSLEVPLDAKIEEEPEEEPEEAEEEKTEEVEAEAEEEAEEKKSEHEEL* Lottia Protein Model: jgi_Lotgi1_202874_estExt_Genewise1.C_sca_21760001 NR Annotation: pred icted protein [Nematostella vectensis] gb|EDO37140.1| predicted protein [Nematostella vectensis] NR E value: 2.00E-54 Sequence: MMKLFVILALLAGQSLADIYLHNPRGSNDRLNEKSAQRANANRLFDSQNNNRGGYNVGDVTSAPHGKDASKQYKMAYFQSEADAETILTVEW YNQHGCGGNEDDNPQKQNCRLVLQYMCQPEGTTEDVLRNGVVTNTQDYNRPPNSNYNLANRNSRKNNNVKADRGLQESW DW YEECFVRERNKGLFTADQNLRGNNGLGYSSAIYTR Lottia Protein Model: jgi_Lotgi1_203670_estExt_fgenesh2_kg.C_sca_170029 NR Annotation: PREDICTED: hypothetical protein [Ornithorhynchus anatinus] NR E value: 1.00E-142 Sequence: MKLSVFLSLLFLTAVWADTPANCTFDDIKGTWTFFIGSNGHDNTVNCSNMGNPKSQLQVTLYFPDVAVDEFGNKGFWTIIYNQGFEVVISGRKYFAFSMYEQSGSTVSSICDKTLPGWSHDTFNRDWACYYGKKITSVPPKYYKKNKPHRLFKLNYDYINNINKQQKLWTATVYPEYEGKPMRELLNKAGGPASQVFGPIKAAPVTW EQQRLSETIPEQFDW RNVNDNNYVSPIRNQESCGSCYAFSSMAMNEARVRIMTNNTLQPVFSPQDIVECSEYSQGCAGGFPYLIGGKYAEDFGLVEEKCNPYKGQDHSCSTDKTCKRQYATSYRYVGGFYGGCNDALMKMEIYRHGPVVVGFNVTDDFFHYKSGIFIHTGLTDRYNPFEVTNHAVLVVGYGTEKGVKYWIVKNSW GEKW GESGYFRIRRGTDELGIESMAVSSTPIF* Lottia Protein Model: jgi_Lotgi1_204607_estExt_fgenesh2_kg.C_sca_690020 NR Annotation: ctg4a [Aedes aegypti] gb|EAT44181.1| ctg4a [Aedes aegypti] NR E value: 2.00E-45

180

Sequence: MAFMFFQTLLIIVSIGYLHADDSDGVQEATLCEVCRILAEELQLRLDETGKSKEVIETGHGLDTKKKKKYNFSELRLIEALQEPNICDRILEYSVHKEKTGIERFAKGRSQTMEALHGLVNKGVKVELGMPHEMWDKPSVEITQMQRKCYALVEEYDEDIEDW YYNHQDQPFLDYFCRNLVLNPQNNECLDEDMRSQNEGSSSEMKGDKGKKEEL* Lottia Protein Model: jgi_Lotgi1_204654_estExt_fgenesh2_kg.C_sca_720011 NR Annotation: PREDICTED: hypothetical protein [Monodelphis domestica] NR E value: 8.00E-93 Sequence: MSLLIATGLFCVIYLLVKFLNISQPCHLPELYFKDKTSNFIQTVFTMCPILFESYIPPLLW GKSGHLQTFIYAKMGRIMSPLPNGKRIELIRPDNATMSFDVFQPHYEHPTGRDYTLAVCPGIANSSESLYIRTLVDHAQYNGFRVAVLNHLGALRKVKLTSPRIFNYGETGEYNCMIDELKRLYPNTSIIAVGCSMGANIVIKYLGECKQHENKVIGALSFCQGYDVNDAKPFLLGW ESCRRLYCYFMAINLRQLLRSHQDILFTEQAQSEYGTVELKKLYSATSLLAIDELYSVNAFKFKCCEEYYHWASCKQYIQNITKPIFILNAEDDPIVPKQLYTTPLKYQENHDNCLFIVTKHGGHLGFFEGGFIKPNTITW LDRAVVQFSNVLCELDCKERKS* Lottia Protein Model: jgi_Lotgi1_205030_estExt_fgenesh2_kg.C_sca_1120002 NR Annotation: PREDICTED: hypothetical protein [Strongylocentrotus purpuratus] ref|XP_001188672.1| PREDICTED: hypothetical protein [Strongylocentrotus purpuratus] NR E value: 3.00E-20 Sequence: MKLMFLGFIGILMLSTSNNASASPWFCKGLECPKFNLLNKTKDYEVRQYGSLTW VTTSHIGQWKSDILGELLFKNLFSYIEGNNTMKKQLDLAVPLLTKLESAGDRGSIYTMHLLIPRENIDHLPSPLNTNLTFITIPPSTYYIRSFDGVAYDRQYMKVLQELVNAINDTGAYPDDFFYTATYDHPQVKENRHNEVW LLKKEKIP* Lottia Protein Model: jg i_Lotgi1_67930_gw1.39.124.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO32094.1| predicted protein [Nematostella vectensis] NR E value: 1.00E-10 Sequence: VGHFVLSCCMW RAVFSCCMWRVVFSCCMWRAVFSCCMWRAVFSCCMW RAIFSCCMW RAVFSCCMWRAVFSCCMWRAVFSCCMWRAVFSCCMW RAVFSCCMWQHVASCIQLLHVASCIQLLHVASCTQLKHVASCIQLLHVASCTQLKHVASCIQLLHVASCIQLLHVASCI Lottia Protein Model: jgi_Lotgi1_98330_gw1.10.599.1 NR Annotation: hypothetical protein NEMVEDRAFT_v1g147894 [Nematostella vectensis] gb|EDO28414.1| predicted protein [Nematostella vectensis] NR E value: 5.00E-06 Sequence: TVCSLGTHCLSITVCSLGTHCLSITVCSLGTHCLSITVFCSLGTHCLSITVCSLGTHCLSITVCSLGTH CLSITVCSLGTHC

181

Object 2-7. Predicted secreted signaling molecules found in Pleurobranchaea californica.

Likely to encode functional secreted signaling molecules

Lottia Protein Model: jg i_Lotgi1_167943_fgenesh2_pg.C_sca_71000005 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO34356.1| p redicted protein [Nematostella vectensis] (Fulicin precursor) NR E value: 1.00E-21 Sequence: MKSILVTSVVVVAGTLLTLVQALGADKFAEPATQKHSKIPTAALSHSGDTLLNTDAHQTNPSNIWKSKSKFDLDALPVVNRQDVQSHYAQHRRSYDTPMFLGKRDLKNLSLRFKRATDLEDLDATEFSDDFDKRGVDIPQFIGRRRYDTPQFIGRRGYGVPAFVGKRAFRAPAFIGKKAFGAPAFIGKKAFDAPSFVGKRKLTAPSFIGKRGLDVPAFVGRRAYAPPQFIGRRGMDVPAFVGKREFQTPMFLGKRAFSAPSFVGKRGLDAPTFIGKRKEESFRDLLATLQAYREYRRLMQTDKRFDAPLFVGKRSDYENDNLAAETLSRRFQCIPQW VFRMRLNVVDLNMSFIGGYKQNYFSLNILDDVQTKILLPLDDLHKVDSSKMDVPTVQLWDADMPRPNGARFLKLDPAHALGFLGQEASPVSKRYLEFVGKRDNDKRYAEFVGKRAGFGEKRYSRGSLSEYLSKRLRIRRPEFVGKRSNQKRYLEFIGR* Lottia Protein Model: jgi_Lotgi1_168619_fgenesh2_pg.C_sca_77000042 NR Annotation: crossveinless [Saccoglossus kowalevskii] (a new family of twisted-gastrulation-like modulators of bmp signaling) NR E value: 1.00E-148 Sequence: MYYTELSILAKCIALCILTCFPILIQAQLTGTVTRCYNEGGEVKIQGITTDPCISCYCMDGYVQCDKKKCDNLDGCHAILFDGPKRCCDTCKGCRLNGVKHESGKSWFDKRDRCTSYSCKAGVITKSKVQCHVPCSNPIKRKRQCCPSCKGCHFDGADRKNGEKFALASDPCVECKCRKGSVTCMKRACPVLNCPDEVIYQPKGECCPKCKGMRKIFDFPGACYFAKRVYVDGFSFEPKSETRCTCTKGTVICEKATCPPVACPVEERVQKGSCQVCEPKRNCLYDGKIHMHRSKWQPRMCTQCSCQNGVTYCQRERCNNSLSCPNGYKLQFQPGECCPKCVEHDAVCRVFGDPHYRTFDGKMYNFQGTCKYMLSQDCQGKDFTIKVKNGVRLSSGFAWTQMVVVLMGDTRISFRQNLLIKINRRRVQLPYTIPGKFSIRREGHSVTFRADIGLKVVW DGDSFLEVTASRKYKNRLCGLCGNYNGLETDDLIGRKGKNYLRGEEFGNSW RIGSKKACKTQPKVKNLQSICDKDFKAKVRANKECSVLYSRAFSSCRRVVDVTPYVTSCVTDMCDCPHGKKCSCESIQAYAHECKRAGHKVKW EKVSNCKAPQKNCPKGAYYSLCAPACPKTCSSNKPDGSCSKKCSPGCVCQNGAVLYKNRCISPEKCPKT* Lottia Protein Model: jgi_Lotgi1_169325_fgenesh2_pg.C_sca_84000054 NR Annotation: Insulin precursor [Contains: Insulin B chain; Insulin B chain'; Insulin A chain] gb|AAF80383.1|AF160192_1 insulin precursor [Aplysia californica] NR E value: 4.00E-13 Sequence: MEVTCKCPLVLLGVLFLNFGTVLTHLEWTCTLETKRESPRGVCGQRLPEVLSMVCKRYGGYRDTWFRKRNGEGTNSRLGNIILGKRDAFSYLGKRGQSYGEQGITCECCYHSCSFRELRQYCRNSQQRISIKK* Lottia Protein Model: jgi_Lotgi1_179503_fgenesh2_pm.C_sca_71000002 NR Annotation: Wnt7 [Euprymna scolopes] NR E value: 1.00E-122 Sequence: MTVSPTVVWAAFSMALSSVVALGANIICNKIPGMAPKQRAICRSRPDAIVSIGEGAKLGLTECQYQFRFMRWNCSTLDSNDSMFGYESLGGTKEAAFIYAMTSAGVSYAITQSCGLGSLPNCGCDKDKGDGKLAPQGWKWGGCSADIKHGLRLARKFMDAREIAQNARSLMNLHNNRAGRKAVKDNMGTDCKCHGVSGSCTMKTCWTTLPPFRKIGDSLKKRYKKSKIVVPYLGRRARTAVTLILKRAKRPHRKPRRSHLVYLDKSPNYCDFDGKTGSLGTVGRKCNRTTKDTDGCDLMCCGRGYNTHQYTRTWQCNCKFHW CCYVNCNKCSERTEEYTCK*

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Lottia Protein Model: jgi_Lotgi1_188920_estExt_Genewise1.C_sca_260301 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO37057.1| p redicted protein [Nematostella vectensis] (growth factor) NR E value: 1.00E-109 Sequence: MFLEVFNISATVLCILFLIGHRKCDTIQNSKHHAELMMDLLQPYKNYHGQRLKKSNRHIRECQEIKYGNVTHSKVKVKPDTNISYPLVEVKHLTKEIGSYYYKNPVTIRYQRVDFPLNTLSVLEPGEPGTCREGHGTRSITSKTALSGNCIVGVNGGFFNTTTGACLGNIVSNGRLVQDSQGLQNAHFGITEDGYIYVGYLSELDLITQQFKQLLGGVLW LIRDGEKYIDKSKQMECPDVEETGTIETFIEVQSARSAVGHDKDGRVIMVQVDGKTNARGVNLHEFTDILLELGVVNAINLDGGGSTTTVINHTLVNYPSDKCADGTFSCEREISTILCVHEPECQPNDCNQHGHCVLGKCVCDGYW VGSDCSSLSCPFSCSHHGTCTKDGCICDDGW YGINCSTPCKPGYYGSNCAHQCYCLNHGTCNSQTGHCVCSPGFTGKYCESLCEFGYYGDKCNNLCTCDNGCFCHHVTGSCNLS Lottia Protein Model: jgi_Lotgi1_2084(2009). from 17_estExt_Genewise1Plus.C_sca_10710 NR Annotation: fasciclin- like protein NR E value: 5e-178 Sequence: MSRNLWIFLLILLSIIVIASSRRSRGRW YRGRSGRRSLADRLRDRGTKPSNHRPRQRHGW GRW GSGW GAGQDHRTRVEEELDDLFRRIKDRRYGFNFEDKFW LDFGWNSRNEPKPWWEGPNVCKDEKESIENTSISDTGPISRHFSFKNQVCDQSETSYKCTTKTGIFMKQKTVTEVYECCPGFTRKDEMFGCPTEFKLDGLVKTARNLGLTEFLRATESVSLTDDLEKGNFTVFAPVNKAFEDEKNILPELETILPKDMPSVVMVSKPLTDLVIADMSNVLLGHLVYDTLKTSGLNDEQLIETASPFDSLLRVNFFSRPEKLITVNCARILSADNQATNGIIHTVDRIIKPVTDSIVDIISQNPNLSYLKTALARADLVKALRGTGQYTLFAPTDAAFEKLEPSIRDRLIKGEKSCLEKVLKNHLLTSVICSTVIQGTVTTTNLLNKYLNLTRTEDDKLFVEGSQMVGRDIMATNGVIHVIEDVLVPDQALGLIDVAEKNNLTKFVELLKETGMFKGITKMENLTLFIPSNNAFEKLEEKVSLEELKKDKKKVEDIVMYHVVPEVVTCSRLHNNYKLSTENERNIRMNRYSTFPFRHHSIQTAQCAPIIKENVPACNGVINIVNEVLLPPVGNVVDNLASKKKFSTLVRLMKKSGLADMLQEEGPFTMFAPTNEAFDDLYEEDLEKIEDNPEVLKKVLQNHIVQEFVCCASISMVRSWFDVASVRTLSGVRFPVKERHGTIQFNHAVVTECDNTATNGVVHAIDKVTLSPERPFWKRDFYHW*

Predicted molecules found in Pleurobranchaea californica with unknown function or predicted to be related to the secretory pathway

Lottia Protein Model: jgi_Lotgi1_107224_e_gw1.6.194.1 NR Annotation: No match found NR E value: Sequence: CFLSVCLCSSICFTTECCFLSACLRVCFTTECCFLSVCLLFKRSRVCFTTECCFLSPCLRVCFTTECCFLSVCLLFKRSRVCFTTECCFLSVCL* Lottia Protein Model: jg i_Lotgi1_118077_e_gw1.28.72.1 NR Annotation: LOC100124858 protein [Xenopus tropicalis] NR E value: 1.00E-108 Sequence: MGSTLNILVILGCILVPFSFSNFLEW GPDLEGPW CATRPIEQCCPGRDDECTVPILGTKCYCDIFCNETAEDCCPDFW NLCLGVTRPTPWPLTTTTSRVPINILCNVYW FKCTKLHFSSHWECSNDDCLLEADHITQINNGPYSWVASNYSDFW GLTLEDGVKYRLGTFPLGSNVVQMTPLRVKLTDVLPESFDARTKWPSYIKPIRDQGNCGASWAFSTTAVASDRLAIESLGEIKDELSAQHMLSCNVRKQKGCEGGNLDRAWWFLRKTGVVTEECYPYES G

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DTTDKGDCLVSRRKSNTTCPSRILYKEKRRYKATPPYRIAPKEREIMKEIMDNGPVQATFMVKSDFFMYKNGVYRYSNISFDEEPAVYHSVRIIGW GVERTIYGDILKYWICANSW GTQW GENGYFRIIRGRDECEIESYIVGVW GQISGDASLRTLLTRGRRRRLFAERSLRNLRVNSMAKGRKSRSRQVGRKKVKRGRKSREERRRLRQEHRNKRKLRRQSRKQDSDSQNTV* Lottia Protein Model: jgi_Lotgi1_118302_e_gw1.28.365.1 NR Annotation: No match found NR E value: Sequence: CVMLYMLCKCSSLDCVKLYVLCECSICHCVKLYVLCECSICHCVKLYVLCECSICHCVKLYVLCECSICHCVKLYCSICHCVKLYVICECSICHCVKLYMFCECNICHCVKLYVLCEV* Lottia Protein Model: jg i_Lotgi1_134336_e_gw1.89.95.1 NR Annotation: apolipoprotein D [Branchiostoma belcheri tsingtauense] NR E value: 9.00E-41 Ac Annotation: CNSN01-F-078834-501 Ac E Value: 2.00E-25 Sequence: MERILTAVLLCIGVAVYAQVPGLGPCFDAKPQSTLNVSQYLGHW YEITKLPSFFEIEQVCLTANYSVKEDGHIKVFNQGKVNGKNVSSTGDAYVPDPSEPSKLIVKFPQAAPYGPYW VLDTDYTSYSLVFSCEQLGSFAHAKFLWILSRTPSIDPAVKDRLFKRLEKDGIGASGLRATKRSDCS* Lottia Protein Model: jg i_Lotgi1_143169_e_gw1.194.72.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO36917.1| p redicted protein [Nematostella vectensis] NR E value: 1.00E-28 Sequence: MVKVCSIPLTVVKVCSIPLTMVKVSSIPLTISKVCSIPLTMVKVSSIPLTMVKVCSIPLTMVKVCSIPLTIVKVSSIPLTIVKVCSIPLTMVKVSSIPLTIIKVCSIPLTVVKVCSIPLTVVKVCSIPLTMVKVSSIPLTMVKVCSIPLTVVKVSSIPLTMVKVCSIPLTVVKVCS Lottia Protein Model: jg i_Lotgi1_145599_e_gw1.360.11.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO49883.1| p redicted protein [Nematostella vectensis] NR E value: 6.00E-16 Sequence: LVVYCHCGLVVYCHW ELVVYCHW GLVVYCHW DLEVYCIVYCYW GLEVYCHW GLEVQCYW CLEVYCHCDQVVYCSW ELVVYCHW GLVVYCYW GLEVYCHW GLEVQCYW CLEVYCHCDQVVYCSW ELVVYCHWGLVVYCHW GLVVY* Lottia Protein Model: jg i_Lotgi1_151060_fgenesh2_kg.C_sca_80000004 NR Annotation: LOC431836 protein [Xenopus laevis] NR E value: 1.00E-140 Sequence: MDFARLFQSLCICLLTLDIQLFGLTSGANPEVEQHLEMGKKLLAAGQLADALSHYHAAVEGDPDNYMTYFKRATVFLALGKSRSALPDLAKVVELRPDFTAARIQRGSVLLKQGKYKEAKNDFETVLKTSPNNEEAKRNIDKIETIPPDIELAYRLVEEGNYGDAIQVLGGIIDTCPWDPTFHEIRSECYEAQGDLFKAASDIRQSTKLRADNTRGYFKLSSLHYEMGEADESLVQIRECLKLDPDNKQCFPHYKKVKKLVKLMTSAQESINNEEYDKCVKKAEEMLKVESLVHHYVSRAGQHKCKCYSKGGKVKEALAECTHLLKIDPNNLDALIDRAEAHIFNEDYEAAINDYQEASNIDGENRRVKEGLNKANKLLKQSKKRDYYKILGVKRNAKKKAIMKAYRKLAVLW HPDKFTTDEEKEKAQKKFLDIAAAKEVLTDPEKRAQFDNGEDPLDPEEQSGHGGNPFQQGFNPFGQGFSGGSFKFHFN*

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Lottia Protein Model: jg i_Lotgi1_153437_fgenesh2_pg.C_sca_3000340 NR Annotation: hypothetical protein PB105991.00.0 [Plas modium berghei strain ANKA] emb|CAI04701.1| conserved hypothetical protein [Plas modium berghei] NR E value: 8.00E-09 Sequence: MKTMVAIIGVIIVSCVLHTSGLPLFKNILNERSVEYGGESGNGFKNERAVGYGGESGNGFLNERSVEYGGESGNGFQNERAVGYGGESGNGFLNERSVEYGGESGNGFQNERAVEYGGESGNGFLNERSVEYGGESGNGFQNERAVGYGGESGNGFLNERSVEYGGESGNSFLNKRAVEYGGESGNGFLNERAIEYGGESGNGFLNERAVEYGGESGNGFLNERAIEYGGESGNGFQNKRAVEYGGESGNGFLNERAVEYGGESGNGFLNERSVQNSGKSGNGFLNERSVQQGGKSGNGFLNERSVEYGGESGNSFLNKRAVEYGGESGNGFLNERAVEYGGESGNGFLNERSVQYGGESGNGFLNERSVQYGGESGNGFLNERSVEYGGESGNGFLNERSVEYGGESGNGFLNERSVEYGGEAGNGLK* Lottia Protein Model: jg i_Lotgi1_154590_fgenesh2_pg.C_sca_6000093 NR Annotation: PREDICTED: similar to meteorin, g lial cell differentiation regulator-like [Tribolium castaneum] NR E value: 3.00E-24 Comments: Secreted, involved in both glial cell differentiat ion and axonal network fo rmation during neurogenesis. Sequence: MDFLQATWLLVVMVIFHIYIITADQICNQCDCTIRDSTGKGNINVKPKCQSGKITWPDPVSAVRLELKPEVVGPFKACIALKSENIVTLVSREITTETVNYRTRSYDSKVDLLPWFSVEGQGKEHCVTSSESSVMLYLEIESTTEDSGLSRTFIYYDIELIPDNKFSPDPMEECQPCSDDEILRSYCISDYVVVGRMKEVNFQSELDKTKVNIIVDQIVKTTDEDIFKRHDNLLEGALFTSVHCGVQKGPGQFLFTGRNRLGQPILTCAPFYDQWIQIQRLAQEKGTIECAYG* Lottia Protein Model: jg i_Lotgi1_158797_fgenesh2_pg.C_sca_18000147 NR Annotation: predicted protein [Monosiga brevicollis MX1] NR E value: 5.00E-92 Sequence: MKIHILAVLFSFFSATNAATLRFVSQTPLSITINKDGSYTVNVNGEPW LESSAIYFYAEGRRVSMGDGSLKIASTFMDNGYDSVGQYGKTCW RFEIESKYIDTCFKQYMEPTPFIIFEQHYLNDTENTASIDYDYTIAGFPAFTAPPTGKLGYLHYAGSMTGDSDKSFGIWQEKAKMLTGVAGGPLVLFDKDADDCLILSPLNQFMAASSLFDGQNYSTGVLGGVNFVPKDFVLQTVLFYGKGINHAMSDW GLLLTQYYGKDDSYRKSDLTLNYIGYW TDGGAYYYYNTEPNKTYQQTILDVRQNAKDIGMPYKFVQYDSFFYYKGPHGGVKTW VPMPDLFPDGFQYLYNQTGW PAGAHNRWWSPKTTYARQNGGNFDFIVEEAKAVPTDQAFWDYLFDSSKKW GLTLYEQDW LDNEFAGVKALLTQIDLGRTW LMQMGRAAQENGITIQYCMSNPRHIMQSVEIPVVTQARASGDYRAGKDNW KIGISSIMVDALGVAPFKDNFWSSPDQPGNPAYPDLKETHADLQVAVSTLSTAPVAPGDMLNKTNVPLVMQCCNADGMLLQPSKPAKAIDAQILQLAFGGDVGPQGDIWTTFSNIAGFYFGIILGTDMTADYNLTPSKASFKAFLPSEVFPRFASIPKSQEFSASNPLVITTNCTKTNICLYYTSPVFSPGGTNVLIYGEENKIVPMSPKRVTDITLDRDLKLTLTGAAKESVRFGFILPDQYITITCQLGQSGSAVLSFNERSCNGY* Lottia Protein Model: jgi_Lotgi1_160458_fgenesh2_pg.C_sca_25000063 NR Annotation: predicted protein [Monosiga brevicollis MX1] NR E Value: 6.00E-86 Ac Annotation: no hit Sequence: MNYKMILNKYTLVLGIILVCTQPCITDVNITKVHLIFMNHLDVGFNGITPQTGFVINVINKYFTKYFPGAIELALQMNFLGYRERLIYTTHPW LVSLYLDCPQNLTMSGIQLMCPTEEQREGFLYAAKRGDITW HAGPMNMQYEALDTAMVNFSLKLSEDMDARMDIKRRHRVLSQRDVPGMTRALIPIFNNHGIEGVTVGVNSVSSPPAVPKIFKW VFQNSSVIAMWHPGGYPIKPGSFPSMPLGLSRDNCVTFEQFDEAMCFSFRGDNQGPPTSIAEVLNSYEIVRGQFPGAIVEGSAFENFVEVVQSVKDKLPVVDQEIGDTWIQGISSDPGKTAMMRGFFRARTACLTSAPNFEDVISSWSEQRQFIDLALETLGDHPLVQAVEEEYKQLVPQAPDLSKYKQLSATTFDCQNGFKFSFAADGSL

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NKLQDKDGRNWASKSQPIGQLLYKTYNDTDFDSFQKQYVLNHYYW FQVGKWNLTANCPTCESTIWNTKLQKLYAASDGSCDVVALLAMEEDRATSFYGAPPKIYVKYLSSSNTFLSIEVQLFGKKPTRLAESLSLSFQPIRQEGSKW YLHKLGQLIDPLNVVTNGSQRLHAVDEGVQYKDSNGKGMMIGSKDVGLATVHQTPTDVSVLPVPLTPIQSISGVSYNIFNNVWDCNYIFW YPFLKQDSNSKYRFSITFS* Lottia Protein Model: jgi_Lotgi1_161657_fgenesh2_pg.C_sca_30000077 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO44809.1| p redicted protein [Nematostella vectensis] NR E Value: 3.00E-17 Sequence: MTKLSEYFLICSMIFVVFMLQRGTCDDVVTEEQISYAKGSVCGYCSYCKFCKLCDKDCPCETSPSKPNCKMCKYCKYCYICSGVCDTICQPGGMIDKISAAIVNALPSFNKEEIEDDLESVKPW LDKKDEL* Lottia Protein Model: jg i_Lotgi1_163300_fgenesh2_pg.C_sca_39000034 NR Annotation: PREDICTED: similar to TTC17 protein, partial [Bos taurus] NR E value: 1.00E-133 Sequence: MASSVVVIVLLHFLFEQCLLTRGSMHW VVTEDGRIQSQADSVYNLRRPYDLVAFMHQEDRATVLNHLKKELLNRKGEIDKSEDRDTTGLEQKFYKSDYDCLQAGTPLPEIDLYISTILPIEHKHISDELSIEIQKAAQSSDNKIGTIPPEPDCTKVLQLDFTPHGFEHLEGVQERMNLTGSAELGLTHAIPFIPDKTEIPDFGHVIAKELKKNSTSWVLYNMAAFYW RVKGDPWMTVECLRRALHFSPRENKDIALISLGNILHRARYSNEAAIVVHSALELSKELNVNHFTLGNIYAVLGEYNKSIICYENTLRIQPDFEAATIRKHAVLCHQKLEAALEAQHRSLQKTLRDLKDYQKKHDLWHIQNDRMSVEQVSQEEKVSQNIAFEFNKAKQTSTKDIGEYCNMVEREGKQVLLCTWNRQAPTLEMLDQFALEEQKKESERNRLKLNKYEKKAIDYNLPVRAPLYVKHDRQAVPRFNELELDNTWPEKEECDSHLLTSPDPFNLSTVYLSPENKGFEVKALLTEAQNLKDGAEHPLPW YPPICVTLLTINEYDEKTYDNLKSVGHPGRTKVPLKMYDPSMRKTLLSHVNGGTVTEEEVGQRILSAIKQEVGARW VLFNLAGLYW RIVGNNYHGIECIRRSLALAPDEYADVPLVNMANILYKW GRYDDAIVLMKDALKINDAEPDSNFLMANLLW VTKNYSGAISHYRTVLDIIPEHQEAFDSLRAIKCLQKFLIKEQSVAPIEVPSQPVGNSCQQKGVANKNQQTESRVICKTENGEEKCIIETRMRGKSGECNGHCTQTCTITPIKVESSCGGPDLAVDTSNPVQCHQKGSQCGEAGEELLFTNDFTNKLDEVSDHYEKKGICNGEDCNTLRVQCLIPMKTHSGLVAHVITPPKLFVRPLSLHSTQCMTEQKKPHIKLEFIDGVLHQKLIFVQDANDIHVEDNECIIFNDGIKSPGCDQAQYRSYSEELARSNINVEFRYVGDESPVEKDKKNHCSKTPNSPGVPSDEIPYSDISDGILQSLNGDVEPSEVVGHRIALTLQQNPKSW VAASAAGLYW RVEGDFTKALDCYRLSLSYAPLEMADLMLLGYANILVQGGYYSNAIMVEDLALDIQSTLPIHYFTLGNIYAALGKWRTAVSFYEATRFYQEDFKPAVDRLRAIHCENIA* Lottia Protein Model: jgi_Lotgi1_164905_fgenesh2_pg.C_sca_48000037 NR Annotation: no hit Sequence: MMTKMLTIIFSVLIVLCSSSPECGPNYFEGDKWFDGCNW CTCTDNKEKCTNATCTEYQDELDNSPCQKLGESWRDGCYHCRCLPDGIRCRANKRCVHALKSDDLNCPVDGLCVCGLDGVPSCNEQIMSRFSFKNVPELGPDTSSDKKLILSSKGENTLRLNPDEAETDLGDKDLHLIPTDDKLCHLGPRW YGGGLRCYCDPQNGVACGNPRFIARKVDGFKIRSKKCRVGKIWEERCRICTCKKSGLSLCRIDRECTKNKNIRHSVKSIVVAGNAECRVGLRW KDGCKRCRCNKRGKIICKTRLCNLSDDGKKLSNKDKTHRQTSTGGLISKSTPRFQLPEKPRFRMPNLSLYNNRFQRKKRPSIKTVNCNGFKPGEKYWDKCNLCHCTMAGAPMCSSKLC* Lottia Protein Model: jg i_Lotgi1_166720_fgenesh2_pg.C_sca_60000088 NR Annotation: No match found NR E value: Sequence: MMLLIKTGVLLIILFSLNTLCDSCACEFHPVLSGKWFFTGENKNINIKIHSKIIVFKVSNYLKLRYKCTERYGNIYLLRILCLFLEVDFLSDLFMKGGFTSIHVEGRGFDSFIFSPKNVTRKYANIDNTCDSEISRERKFIYRKGGC

186

RVPRDMVGTW GFTFRQSSAITMGKRNLTIHFLNNDNLTLNCEARDRNMFLFRRSSYINSSTDAIICVQIDPVLDDPFYDYEFNRIITGNAVENLVRTVPRGKKLYINSDCDMIHSPARPEYIYTVDNIL* Lottia Protein Model: jg i_Lotgi1_167022_fgenesh2_pg.C_sca_63000026 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO43826.1| p redicted protein [Nematostella vectensis] NR E value: 2.00E-08 Sequence: MVAVRIRKYKNLLFFALLFAGVLSISTIFYSNHYQIMNTGTRSDVNTGFSYSNANSSKYVIYICDGKNSCGGYGDRQKGIVASYIISVMMNRQFGVIMNDSCDIKQMFKPNQINWIVDNNDIKSKTSKRIKALDGYSDKLRKSMIEMDLDQEYKEEVIYFSLNLEFVFYLRQNKRYEKQLKW LEHLSMSEIYNVVWQSIFKLRPYIREKLDPFLDLKKQGLKLISAQIRLGKNPTIPHDSRVVNSLNNMNVLW QFYKKYNDSSKYRIFISTDSDTVREKARSIFPDVYSDVPGKVFHVERSNKTDICNGW RKVILDQVILSLSDVLVISNSGFGRIAAFFRQNENDLYCLNLDKIRRCTTQSEIFVDKTW* Lottia Protein Model: jg i_Lotgi1_168040_fgenesh2_pg.C_sca_71000102 NR Annotation: No match found NR E value: Sequence: MVGTRYLQLATSLAVFLLVLFLTCTQAAPVDDFETDNEALRKALFIARLLSSSDRLKNTKPEGSNLDFSELASIPFSNQQKRYRPPMQGRSGGMSLCLWKVCPAAPW LVSKRSEKTWDKNNMLGK* Lottia Protein Model: jgi_Lotgi1_168805_fgenesh2_pg.C_sca_79000073 NR Annotation: PREDICTED: similar to plexin A CG11081-PA, isoform A [Apis mellifera] (a neuronal semaphorin receptor that controls axon guidance) NR E value: 1.00E-121 Sequence: MEGGIRLQLFLLVSLICHTQCIKILHSFRDPGGNILQKLVIDDQTGNVFLGALNRLHKLDPDLNIVQSASTGPRLDNVECPPPLLPCDKPKTQLNSQIKGLVIDSASNSLILCSSLFHGSCQTLALNNITNVKKFFHKPLVPNDHHSCYMFLAPSINNTQALYIATAYSDLGDAAYRDLVPSISSRSLDNLDFIHRDTEGSTKLEILKEYRESFIVRQLYGFQNGGFVYFITLQRENPGSQKIVSRINRLCQQDRYFRSFVEIPIKCQKSDSDSYDVVQNAYFTNGKLYVIFSNTVSSAGAKKSTFCEYDIQDIDLEFNETVRNCYKAVGNFGPEHYKKIEPCPPLIFGSVDFCGESGEW KDYSAIEGSKPISATPLHFFTSDKPTAIFVHPEGNHRYAYIGTQDGHVIKLRFENGQVNQLFKITHSVGDIILQITNHPQSNLLYILGSHKLSVLSLNHCDDLTSCDECMNDGDETCGW CVMDNRCTTKELCQSSVITPPW LSRTDQSCASISSLQPSSLSYKKFINGGDSRQIKFQVNKLKFESNRNLNCLFINGEKHDNTMAILDQKENTITCPLPRQLPTIPQGKDHEDLELQFHVEGKMIVKRDVSVFDCQGNNNCTSCSTSQFNCHWCPVSHSCVEQSESCPQGDSIKVNDRCPRLETAASDTDILVHSGELKTISVQVRALEANQRSNLKCHFDLSGQVQTVSAKISSSTLTCETIKLAYADDIPYIVAGFKVTW GQQNHPLDNPQRMNVRIYKCASMVTNCGKCLSLDVEYECGWCEDQCSLQKKCQKSWLDQSETCPNPQILRYSPSKGPVKGKTEIQVNGINLGKEISDILGQVTVAGLPCDVMQEHYEPSSSFKCEVKGVTSAKSGTVKVVVNNKYTAESDTVFEFVDPVLNHVDPKQGLMSGGTRVLIKGNNLDVGSDTQVIVGGNSVPVIK* Lottia Protein Model: jg i_Lotgi1_170051_fgenesh2_pg.C_sca_92000069 NR Annotation: hypothetical protein TTHERM_00439050 [Tetrahymena thermophila SB210] gb|EAR97557.1| hypothetical protein TTHERM_00439050 [Tetrahymena thermophila SB210] NR E value: 3.00E-08 Sequence: MKMINIFIVVYCLISPCLGFW LSSKTVDPKVDISTECINKALTGDCGFFTCFEERLPCGEYGYAESYGGKYCWQFQQSHHLFTKKGVEFVEKLTRCHMNRSITSYRQNQIECFSHYDQSFVIMGDCYVESGFCDVVIDNFMSLARILEPKDFTNYRVVREVFRAAKQCPNNISGGIISKFLSYKRGSSL*

187

Lottia Protein Model: jgi_Lotgi1_171450_fgenesh2_pg.C_sca_115000014 NR Annotation: No match found NR E value: Sequence: MKRFTLLALLLVAILYISQSESKCQGVTKCMVNLFTCKREQISVNIAADCCKEYFECFNECKPGSRPPCKNTAPGKRGSWNKRYSSRYGSDILGDIISGY* Lottia Protein Model: jgi_Lotgi1_171757_fgenesh2_pg.C_sca_119000045 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO37253.1| p redicted protein [Nematostella vectensis] NR E value: 7.00E-24 Sequence: MWKIFSFHWIYFLFSLFCLLHSVTCVKKVSKTPRKSKESEVSKESTKRSASLDDDPSLWPGHLQPLGSSQQVHKVDAYKKYLKPEEFLNSYAAPYLPVLFQAVEQTSIAYEKW GNITYFTVLPQTLTNW VHISPEVGQVSFRKFLLNYTDKGSVFNNKVPFFMRSAKWEPYRDDVSVPKCLHCEPILNSFSTHKLW ISHTDQSSPIHRRNKDTIHCTFVGSQEFTLISHIEYGDKVPVDYTTIDINKVDFTKFPTLREVEYHKVRVKAGDCLYIPTKW YWQVRSTGKTYSADIEW YHRNKTVKSSNCKTKTSISSLNSVVFKDIDDPTTIETDVLLHYFTTYLLNVPKFTLPQFERHLKKDKKFMEDLIEWTDELQHITKEVFETLDVNKDSRFSMADLESLSQPMLEDIRGLFLDRLQDYDDILKDQQEEAKESKKQSEKENKAKDAPATTGPVDDYLKNYIGQLEDVLKESIEEMSVTGHLPDIKKKFAEKQAQGKTGNADKPKQSTKPKTEREKAKEKLEQDRQRRKEKQEAENIKEKTKDDEKYLIVDDSVEEEIMADDDDDSTTGSTNKRTPTADKADTKDNKSHTKDNKADTRHVDKKTGRKGQDGKEAKQKNTGSKIKTEL* Lottia Protein Model: jgi_Lotgi1_173484_fgenesh2_pg.C_sca_161000006 NR Annotation: no hit Sequence: MRLHYLVTVLVVLILIDVGEGQDNISKCLELPSVKEIVTRYICPKPDPCATVDCNCNGKCVDGKCQCKNGFFGEFCTALTSLIPKVAGFVSNNKSLISSEAQTIGSIAKAGVSKKSKELKELQQIRKLRNRRLEEKKGKDFKDIQKSLGSLLGFPWAKYPGEKHLQDHNYTGPGTRLDLRLDENDKPKPGGNETFTFDFISIMLGKKQFE* Lottia Protein Model: jgi_Lotgi1_176362_fgenesh2_pg.C_sca_1085000001 NR Annotation: no hit Sequence: MVAKGTVGILIVLFNTICANIDLNYNQVPVANPLLFGRRGINPDMSSLFFGKRSGNSDHRDLRKMKDTCKAVLSSCKILFSDYEDDTVRNKVQDGFGRFK* Lottia Protein Model: jgi_Lotgi1_177026_fgenesh2_pg.C_sca_12012000001 NR Annotation: no hit Sequence: MMTKMLTIIFSVLIVLCSSSPECGPNYFEGDKWFDGCNW CTCTDNKEKCTNATCTEYQDELDNSPCQKLGESWRDGCYHCRCLPDGIRCRANKRCVHALKSDDLNCPVDGLCVCGLDGVPSCNEQIMSRFSFKNVPELGPDTSSDKKLILSSKGENTLRLNPDEAETDLGDKDLHLIPTDDKLCHLGPRW YGGGLRCYCDPQNGVACGNPRFIARKVDGFKIRSKKCRVXKIWEERCRICTCKKSGLSLCRIDRECTKNKNIRHSVKSIV Lottia Protein Model: jgi_Lotgi1_197084_estExt_Genewise1.C_sca_820083 NR Annotation: glucose-regulated protein 94 [Crassostre NR E value: 0 Sequence: MKRFIVLGILGFLFIAGAGLLNVQAEEVVTEPDVVEDIGKSREASRTDDEA VQREEEAIKLDGLNVAQMKELREKAEKFAFEAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRFVSLTDKSAMAATDELSIKIKADKASHVIQITDTGIGMTKDDLVKNLGTIAKSGTSDFLSKLGESKDQAQMSDLIGQFGVGFYSAFLVADRIVVTSKHNDDDQYVW ESDSSSFSVAKDPRGNTLGRGTMITLHLKEEAHDYLEESTIRSLVKKYSQFINFDIFLWASKT

188

EEVEEPIEEEEEEKKEEEKKEDEDAEVEEEKEEKPKTKKVSKTIWDW ELINNVKPIWTRKPSEITDDEYNDFYKSISSDYEKPMTKIHFTAEGEVTFKSILYVPNSSPSDTFQNYGKQMEQIKMYVRRVFITDDFEDMMPKYLSFIRGVVDSDDLPLNVSRETLQQHKLLKVIKKKLVRKTLDMIKKIDKDTYEKFW KEFSTNIKLGVIEDTTNRTRLAKLLRFSSSNSDTELTSLAEYLERMKEKQEHIYFVAGHNRAEVEKSPFVERLLKKGYEVLYLTEPVDEYCIQSLPEFESKKFQNVAKEGLKLDDSEKAKERKEATEKEFEPLITWLKDSALNDKIEKATISERLASSPCALVASQYGWSGNMERIMQAQAYAKAKDPSQEFYATQKKTLEINPRHPLIKELKKRVEANKEDETAKDLAVVMFETATVRSGYRITDTAGFAQRVENMLRLSLEVPLDAKIEEEPEEEPEEAEEEKTEEVEAEAEEEAEEKKSEHEEL* Lottia Protein Model: jgi_Lotgi1_202874_estExt_Genewise1.C_sca_21760001 NR Annotation: pred icted protein [Nematostella vectensis] gb|EDO37140.1| predicted protein [Nematostella vectensis] NR E value: 2.00E-54 Sequence: MMKLFVILALLAGQSLADIYLHNPRGSNDRLNEKSAQRANANRLFDSQNNNRGGYNVGDVTSAPHGKDASKQYKMAYFQSEADAETILTVEW YNQHGCGGNEDDNPQKQNCRLVLQYMCQPEGTTEDVLRNGVVTNTQDYNRPPNSNYNLANRNSRKNNNVKADRGLQESW DW YEECFVRERNKGLFTADQNLRGNNGLGYSSAIYTR Lottia Protein Model: jg i_Lotgi1_204770_estExt_fgenesh2_kg.C_sca_820009 NR Annotation: unnamed protein p roduct [Tetraodon nigroviridis] NR E value: 3.00E-35 Sequence: MAATTHKMFLVACLVFLPIFASADTCLGPSVSAETYTTSEVTVSTETVFIAQFTLTCKNGLQNVNLYAEVAGRMIPAVKTSKPNEYQISITDEYKALRSGTYEAKFYDEESFSALKKAKRNNEDSNSVTPLFSINISHSGLWQGSYIQSEFVAACVAILVWW LAFSAKSKLSP* Lottia Protein Model: jg i_Lotgi1_67930_gw1.39.124.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO32094.1| predicted protein [Nematostella vectensis] NR E value: 1.00E-10 Sequence: VGHFVLSCCMW RAVFSCCMWRVVFSCCMWRAVFSCCMWRAVFSCCMW RAIFSCCMW RAVFSCCMWRAVFSCCMWRAVFSCCMWRAVFSCCMW RAVFSCCMWQHVASCIQLLHVASCIQLLHVASCTQLKHVASCIQLLHVASCTQLKHVASCIQLLHVASCIQLLHVASCI Lottia Protein Model: jgi_Lotgi1_173484_fgenesh2_pg.C_sca_161000006 NR Annotation: No match found NR E value: Sequence: MRLHYLVTVLVVLILIDVGEGQDNISKCLELPSVKEIVTRYICPKPDPCATVDCNCNGKCVDGKCQCKNGFFGEFCTALTSLIPKVAGFVSNNKSLISSEAQTIGSIAKAGVSKKSKELKELQQIRKLRNRRLEEKKGKDFKDIQKSLGSLLGFPWAKYPGEKHLQDHNYTGPGTRLDLRLDENDKPKPGGNETFTFDFISIMLGKKQFE*

189

Object 2-8. Predicted secreted signaling molecules found in Tritonia diomedea.


Lottia Protein Model: jg i_Lotgi1_119019_e_gw1.30.87.1 NR Annotation: PREDICTED: similar to seleno protein N, 1 isoform 2 precursor [Canis familiaris] NR E value: 2.00E-85 Sequence: MVLFTLLCCLFIIGRHDPTQIPEEIVINIGDDGVLLFQQHDRDNDGYLSIQEFEPLVYRLLEINVSGPVYDVPISTDDEMITLKSYFIPIVKESMSKDLNDSVSIGLLRTMNSLHGLEKW QNVNLQWMNFGASHFSGFLPKDVDSIMLGSSYFIINVEKGLFNAALSSNRYYPPKVTTNESIIVHRLLTLFHPRPFVVSRFPAQSSVACVRAYNDKYLDIVFRIHAEFQLNEPPYHPFWFTPAQFTGNLIISKDGKHIQYFNLYVPNNKRLNIDMEWLNGPNESENMEVDIGFMPLMQLNSTQSSVPVKNLEEYELLEPLPERPQHKTEGENIEWMATIDLEDAKSSLEKALYPFKKVPYYNFTEAFKKAEDNKKLVHSILLW GALDDQSC* Lottia Protein Model: jg i_Lotgi1_164016_fgenesh2_pg.C_sca_43000014 NR Annotation: chordin [Danio rerio] sp|O57472|CHRD_BRARE Chordin precursor (Protein chordino) gb|AAB93485.1| chordin [Danio rerio] NR E value: 1.00E-119 Sequence: MSPMDLAVCLVLLAATVSSTKFSKIPLKAARENLFGIQPNRPGCILGSKFYGI GERWTPELLPVGEMYCVKCECVPVERKGIIDMKGQTLCKNIKNLCPRPNCLNPVLPKGKCCKVCPDEVNSFEDSFSLGSKSSAPTMISSDSKRRVRNEFVSLLVGKNVRRESVKTSAVAAVYFSIVGRDEIRYSVRYFKLDRPKFLQITDANGNVLFERPIEKKRNGDKKFCGIWTKVPSAYIQYVREERLFAVITASRHFRGLVSGRIMVNRHAKREVFSSVLASPKANGIGGLLSVVYNPRTHLVDYVINLDGVFPGSSEEYFITISKKSKVLHQSKGKASSKTRFIRGSWTLKNKRQSKQLARGRLTIRLTSTNGASVSGDIKPKVTCGVSQAVMSSGNSLEEMKLASSGTAVFELREKGSLDYKIRVTGLTSKVHRIRLEGAVNKKNRRQIIGSVHRNFKADENSFNGW CNGTFKKMTADDLHLFLNNKIFVNVATSENRISELRGRVIALPYHSHFEQVSADPAALGPKTITSNGHAGNAWISMGVGCSLHYDITTTTIAKHHDLTLSLVSPTDIDAYVTIEKISTTNLYEFQQASGSIAVVPESLFRDLDEGSALIQVISGDVILSGNVSVPNTCWQYSQDYDMDILVDEQTSEEVLAANRYKCVYEGKVYENGDSW LPDVNATCNTCSCNKGKIECHRLICPVVECANPIVLDGECCPTCPIETTDQTECKIDGDLRSHPVGSTWHPFLPLMGYAKCVICTCLPGGIYKCERKCPKLDCPASRRIRLNPKDCCQVCAPEEVKPEVVKPLQDVDMKGACSFKDQIYANGAKWHPRVIPFGYMKCIDCSCNNGTPSCSRPKCPKLSCSRKIREDGACCDTCADSDGEEIVEEKKSDSCIFGGKKYDDGETFQPKTSSLSSCATCTCRKGSMKCSLTCPEKCKDPKHSNDPCCKHC* Lottia Protein Model: jg i_Lotgi1_167943_fgenesh2_pg.C_sca_71000005 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO34356.1| p redicted protein [Nematostella vectensis] (Fulicin-like) NR E value: 1.00E-21 Sequence: MKSILVTSVVVVAGTLLTLVQALGADKFAEPATQKHSKIPTAALSHSGDTLLNTDAHQTNPSNIWKSKSKFDLDALPVVNRQDVQSHYAQHRRSYDTPMFLGKRDLKNLSLRFKRATDLEDLDATEFSDDFDKRGVDIPQFIGRRRYDTPQFIGRRGYGVPAFVGKRAFRAPAFIGKKAFGAPAFIGKKAFDAPSFVGKRKLTAPSFIGKRGLDVPAFVGRRAYAPPQFIGRRGMDVPAFVGKREFQTPMFLGKRAFSAPSFVGKRGLDAPTFIGKRKEESFRDLLATLQAYREYRRLMQTDKRFDAPLFVGKRSDYENDNLAAETLSRRFQCIPQW VFRMRLNVVDLNMSFIGGYKQNYFSLNILDDVQTKILLPLDDLHKVDSSKMDVPTVQLWDADMPRPNGARFLKLDPAHALGFLGQEASPVSKRYLEFVGKRDNDKRYAEFVGKRAGFGEKRYSRGSLSEYLSKRLRIRRPEFVGKRSNQKRYLEFIGR* Lottia Protein Model: jgi_Lotgi1_169325_fgenesh2_pg.C_sca_84000054 NR Annotation: Insulin precursor [Contains: Insulin B chain; Insulin B chain'; Insulin A chain] gb|AAF80383.1|AF160192_1 insulin precursor [Aplysia californica]

190

NR E value: 4.00E-13 Sequence: MEVTCKCPLVLLGVLFLNFGTVLTHLEWTCTLETKRESPRGVCGQRLPEVLSMVCKRYGGYRDTWFRKRNGEGTNSRLGNIILGKRDAFSYLGKRGQSYGEQGITCECCYHSCSFRELRQYCRNSQQRISIKK* Lottia Protein Model: jgi_Lotgi1_179503_fgenesh2_pm.C_sca_71000002 NR Annotation: Wnt7 [Euprymna scolopes] NR E value: 1.00E-122 Sequence: MTVSPTVVWAAFSMALSSVVALGANIICNKIPGMAPKQRAICRSRPDAIVSIGEGAKLGLTECQYQFRFMRWNCSTLDSNDSMFGYESLGGTKEAAFIYAMTSAGVSYAITQSCGLGSLPNCGCDKDKGDGKLAPQGWKWGGCSADIKHGLRLARKFMDAREIAQNARSLMNLHNNRAGRKAVKDNMGTDCKCHGVSGSCTMKTCWTTLPPFRKIGDSLKKRYKKSKIVVPYLGRRARTAVTLILKRAKRPHRKPRRSHLVYLDKSPNYCDFDGKTGSLGTVGRKCNRTTKDTDGCDLMCCGRGYNTHQYTRTWQCNCKFHW CCYVNCNKCSERTEEYTCK* Lottia Protein Model: jgi_Lotgi1_208417_estExt_Genewise1Plus.C_sca_10710 NR Annotation: fasciclin- like protein NR E value: 5e-178 Sequence: MSRNLWIFLLILLSIIVIASSRRSRGRW YRGRSGRRSLADRLRDRGTKPSNHRPRQRHGW GRW GSGW GAGQDHRTRVEEELDDLFRRIKDRRYGFNFEDKFW LDFGWNSRNEPKPWWEGPNVCKDEKESIENTSISDTGPISRHFSFKNQVCDQSETSYKCTTKTGIFMKQKTVTEVYECCPGFTRKDEMFGCPTEFKLDGLVKTARNLGLTEFLRATESVSLTDDLEKGNFTVFAPVNKAFEDEKNILPELETILPKDMPSVVMVSKPLTDLVIADMSNVLLGHLVYDTLKTSGLNDEQLIETASPFDSLLRVNFFSRPEKLITVNCARILSADNQATNGIIHTVDRIIKPVTDSIVDIISQNPNLSYLKTALARADLVKALRGTGQYTLFAPTDAAFEKLEPSIRDRLIKGEKSCLEKVLKNHLLTSVICSTVIQGTVTTTNLLNKYLNLTRTEDDKLFVEGSQMVGRDIMATNGVIHVIEDVLVPDQALGLIDVAEKNNLTKFVELLKETGMFKGITKMENLTLFIPSNNAFEKLEEKVSLEELKKDKKKVEDIVMYHVVPEVVTCSRLHNNYKLSTENERNIRMNRYSTFPFRHHSIQTAQCAPIIKENVPACNGVINIVNEVLLPPVGNVVDNLASKKKFSTLVRLMKKSGLADMLQEEGPFTMFAPTNEAFDDLYEEDLEKIEDNPEVLKKVLQNHIVQEFVCCASISMVRSWFDVASVRTLSGVRFPVKERHGTIQFNHAVVTECDNTATNGVVHAIDKVTLSPERPFWKRDFYHW* Predicted molecules found in Tritonia diomedea with unknown function or predicted to be


Lottia Protein Model: jgi_Lotgi1_103075_e_gw1.1.1164.1 NR Annotation: PREDICTED: similar to CG31650-PC, isoform C [Apis mellifera] NR E value: 9.00E-50 Sequence: MNVYYNILILCLGVYCTLGEKEKVPNEPVDQKHTKDGEHNPHYDHEAVIGSKQLEHEFDELPPAEAIQRLENLVKHHDLNADNIITHDELKKW ILASYMSLDEEESDEKFQENDVDGNGNVSW RELVKKEFGYDLEDIEDFKKNTDKQEPDTKEFLLMLDEEEKRFMTADTDNNGNLEKQEFRAYYHPNEYPHMHGVEIERALRTHDKNKDNQVSKAEFIGGLLFLEDREVYLTEEENFNSFDLDGNGKLSQDEVRAW VIQDNNQAAEDEVLHLISQADDNKNNELTLAEILEHYDDFVGSATDYGHKLSDEL* Lottia Protein Model: jgi_Lotgi1_118302_e_gw1.28.365.1 NR Annotation: No match found NR E value: Sequence:

191

CVMLYMLCKCSSLDCVKLYVLCECSICHCVKLYVLCECSICHCVKLYVLCECSICHCVKLYVLCECSICHCVKLYCSICHCVKLYVICECSICHCVKLYMFCECNICHCVKLYVLCEV* Lottia Protein Model: jg i_Lotgi1_132441_e_gw1.79.137.1 NR Annotation: PREDICTED: similar to CG11081-PA [Nasonia vitripennis] NR E value: 4.00E-72 Sequence: MAWKIYVLFVCVLCLRFILAARENYIKATFEGAKDRGIFNKLVVNRETNELYLAAVNHLYKLSPNLEVIQEVVTGPHLDDPSCTSLTNLTCDPDLKSCACKLRPYYTKALVVDYVNKKLITCSTLYHGHCDKLDLDNITNKQMFPFMPMVANNATASTVMFIAPGPAVVKDEPQRQVLYVAASYTRTGLKAYREQVPAFCSRSLEDFNLLLKNAFGSSSIEIESQHRDTFPVRYIYGFGSDNFSYVLSIQKANVQTDRYVTKISRICQSDDDYYSYAEVGLSCTMQNTEFNLLQAAYVGKSGTKLARSLGIPTTEDVLYTVFSIGDSNSANPSATSAMCVYSLRDIRKKFTENIQECFSGIGNTGPDHMIQSSKCLKVVSSHFFVIFFLESFESADLSVL* Lottia Protein Model: jg i_Lotgi1_151060_fgenesh2_kg.C_sca_80000004 NR Annotation: LOC431836 protein [Xenopus laevis] NR E value: 1.00E-140 Sequence: MDFARLFQSLCICLLTLDIQLFGLTSGANPEVEQHLEMGKKLLAAGQLADALSHYHAAVEGDPDNYMTYFKRATVFLALGKSRSALPDLAKVVELRPDFTAARIQRGSVLLKQGKYKEAKNDFETVLKTSPNNEEAKRNIDKIETIPPDIELAYRLVEEGNYGDAIQVLGGIIDTCPWDPTFHEIRSECYEAQGDLFKAASDIRQSTKLRADNTRGYFKLSSLHYEMGEADESLVQIRECLKLDPDNKQCFPHYKKVKKLVKLMTSAQESINNEEYDKCVKKAEEMLKVESLVHHYVSRAGQHKCKCYSKGGKVKEALAECTHLLKIDPNNLDALIDRAEAHIFNEDYEAAINDYQEASNIDGENRRVKEGLNKANKLLKQSKKRDYYKILGVKRNAKKKAIMKAYRKLAVLW HPDKFTTDEEKEKAQKKFLDIAAAKEVLTDPEKRAQFDNGEDPLDPEEQSGHGGNPFQQGFNPFGQGFSGGSFKFHFN* Lottia Protein Model: jg i_Lotgi1_160631_fgenesh2_pg.C_sca_25000236 NR Annotation: Substrate-specific endoprotease Tex31 precursor emb|CAD36507.1| substrate-specific endoprotease [Conus textile] NR E value: 4.00E-29 Sequence: MNRLCFLLVVW QIALSQSKVVSSVEGFNGNLLRIVRSANNAAPQGNLNYNSNCNEAYKNISSSHSMCLVDVCTAVALSQEEIDAAVAAHNDYRKNVSPKAAAMQKMVW DDELAEIATKWAQQGRAGHDTKDARKTISLKGTYIGQNAAAGQANLIAAIKAWHDEVVDFKYGVGSINGKDVGHYTQVVHHQSTRVGCGKAHCPDSTYKSYYVCNYAIGQMGLTHPYKSGSSSCSDCPSSCSDNLCDCNGKICFNGGTLDINTCTCSCPSLYSGDQCQTLQCTKTEKSWCRRSYTVADCTKYSNFPIDCNMMCGVCPYPCDGKQCENGGTLDPNTCQCTCKDPYTGPTCADKSCPGQEEWWCQKFFTAADCTKYSNFPTDCNIMCGVCPACKITA* Lottia Protein Model: jgi_Lotgi1_164905_fgenesh2_pg.C_sca_48000037 NR Annotation: no hit Sequence: MMTKMLTIIFSVLIVLCSSSPECGPNYFEGDKWFDGCNW CTCTDNKEKCTNATCTEYQDELDNSPCQKLGESWRDGCYHCRCLPDGIRCRANKRCVHALKSDDLNCPVDGLCVCGLDGVPSCNEQIMSRFSFKNVPELGPDTSSDKKLILSSKGENTLRLNPDEAETDLGDKDLHLIPTDDKLCHLGPRW YGGGLRCYCDPQNGVACGNPRFIARKVDGFKIRSKKCRVGKIWEERCRICTCKKSGLSLCRIDRECTKNKNIRHSVKSIVVAGNAECRVGLRW KDGCKRCRCNKRGKIICKTRLCNLSDDGKKLSNKDKTHRQTSTGGLISKSTPRFQLPEKPRFRMPNLSLYNNRFQRKKRPSIKTVNCNGFKPGEKYWDKCNLCHCTMAGAPMCSSKLC* Lottia Protein Model: jg i_Lotgi1_165259_fgenesh2_pg.C_sca_50000100 NR Annotation: hypothetical protein CHLREDRAFT_144736 [Chlamydomonas reinhardtii] gb|EDP05543.1| predicted protein [Chlamydomonas reinhardtii]

192

NR E value: 6.00E-23 Sequence: MKVLLVLDICCLIGLVSVGEAVCPDNCNYNGVCDVKHSRCNCYEGFTGSDCKTDCRCNGHGTCQSGSVCKCDEGWKYSGGQCVW DCHCLNGAKCIGPGECGCVHNCKMGNCRNGQCQCW NGYKGSDCSEYDPTM* Lottia Protein Model: jgi_Lotgi1_166436_fgenesh2_pg.C_sca_58000065 NR Annotation: PREDICTED: similar to MEGF6 [Strongylocentrotus purpuratus] ref|XP_001187830.1| PREDICTED: similar to M EGF6 [Strongylocentrotus purpuratus] NR E value: 1.00E-26 Sequence: MMKILEILLCLSFFRATVQQFDCSVCKVCDNSTCIECIEGRYGISCGSFCSLKCSGRCRQSDGYCTSGCDEGYWGPGGLCDQTCSNCNPGGCVMNSGLCIAIGCRTGW YGTTCNNKCNSNCDGNCNQSNGNCNQCIPGKYGTDCQLDCSNCKDTCNKNNGVCNNGCKEGLW GTSCTKTCPNCQSSGCDIDNGNCNNPGCRIGW YGDSCNDKCNERCNDGCKQDTGYCNKCNKGVYGNVCDKNCNKGCLDGCNIDGSCKCKSTYYGTNCSKQCSSNCVDSLCNEISVGYNSCIRGCVAGYKGLYCDKQCPSSCPICNRFDGSCTECSKGFYGTECQLKCPEQCDKCKIKEGTCESCNPKYHGPVCSKRCSDKCLNGECNGVGGRCIDCIPGTFGDNCERSCSNGCIGDTSSTTCDRNGSCISGCINRIYGEKCNLNCSKNCYGSCNRSNGYCNSCLSGYFSDNCNQKCSIHCNGDCDRSTGNCGKCNEGYFGDNCNQNCSINCNGGCQKSSGSCLECNAGYYGDYCKQQCNDKCINNECLRNGQCRFGCMNGKTGSQCDGNCNSNCTICLQTDGNICKECKPGLYGISCDKLCNNTCKKVNSKPACNITTGKCTTGCIDGYKGYYCQDTCLDNCISCNNNQCTSCNTGW YGNQCQTKCNSNCINRCDQATGDCLDGCIDGKSGKHCKQEIG* Lottia Protein Model: jg i_Lotgi1_166645_fgenesh2_pg.C_sca_60000013 NR Annotation: No match found NR E value: Sequence: MGASILLGLTILLSYVNQVHLQMFHPRDISRGGQQQEEQLRRGYLIQILKSFLNRRQEDATEKRKRSCNLNLGFHCQTDEYSSIADMYDFLQSALSPGKRKRNVKIVSIEGS* Lottia Protein Model: jgi_Lotgi1_166736_fgenesh2_pg.C_sca_60000104 NR Annotation: hypothetical protein LOC500991 [Rattus norvegicus] sp|Q5XI89|FA55D_RAT Protein FAM55D precursor gb|AAH83798.1| Hypothetical protein LOC500991 [Rattus norvegicus] NR E Value: 3.00E-25 Sequence: MALTKKLVLICCLIIATVFGLKIMHTIKYQINMRPNIEPIPFYSQNTPKRKLMFPERQSPSLEDYNMKHDDVRSEDIFNKFLSENVVYVKDTTADDYFIEGEKELLEEGEIKDLRDLPSSNYSIISTKNTVFNQYEAVTVKIQLYNGFGQLLKRGGDDVRVW ITGENFQAGGEVYDLHNGTYVAEVKAFWTGKSKIHVSIAYSREELRILLRTRRESKVLRYSVGRFLRGGDTFMTLCSPFHDIPLYPRVCNFTNFNLNPYYCGQPNSPKVACSDLRILHTINYSKQRMNNLESKILSRQNRVNELKSKSITINIGTVVNLPYCSSYKSRFTWTNTAPLGYFNNNTWHPSFCQGQITRQRLSRCIRDLHVYLVGDSTVRQIFQSFLRLTKCTNTTFIGNRPHDRVQRYCDVHDSNFRVEYGIHGLPYSMVRTPDLLNNTKAIGFHLDGLQWSQNKDKMFVMVHLYAHFLFFHTSLFEARVRSLKVPLTRLLNRYPKIKIFIKGPHAFTGADFKPIVKSDYFGKVYRSILLKELFEFRDRVYYLDYWDMSVSSGNRLLHPVNSFVDEMVKFFLNLACDK* Lottia Protein Model: jgi_Lotgi1_172278_fgenesh2_pg.C_sca_130000017 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO35011.1| p redicted protein [Nematostella vectensis] NR E value: 2.00E-26 Sequence: MGVKLLFLGICLIYLVLDLHDAEVTAVSDRQAKLKEKDAREKLQQELSKNYAVKQRRRKKKKFRDPRTRGKSSKVVNRGGAPKVQNGDDGGDDPHIGNRENYLQKYFKGKMW GIRDKKKAPKGNW RKKLRKQKHKKKEKRQNMKKKEKSKRTKVKKFKKKRKKHKKRRRGKRSTDENEDDPGSDQEHTHRTKRYAITDQERMW DYGVVPYSFSSDVTDVMARTYRGVFDALEKRTCISFVEW DGTESFYSNRSLNHMVHLNFVDQGGCWAYLG

193

RYTYRTTNIAQDISCCYVGRTCLHEIMHSLSMEHEHTSPLRDGW LRVNW DNLNEDGQFQFSVIDPLMLGRESW GFDIKSFVSYGTTEFTKNGMMGFQPLMPDFYPTGNSYW QPFKEVQMNYDCFGIKCSGMEEPVCENDGYPGYYEGECGCVCPPGLDPATNCASIKPTDPIRGW PPSSFGLPEPIEGCPAGFQLGSLTQPLAGDSEVSSSSHINAGFENSTAEFGFCLKDTASQTEGGLTEWPRGQYCIHSYSNACPPGFQEGLITYDDSVNATRTILTESPLPDGSFVNNTELW YCCRSDGMVQLDIKLPNTESFILYRHGTAECQNVEGMYMFPEYYSLMIEGETIRSISVYQTLGSVPYNVLDGNELFVYYCYYSPLNKDCGEVIHLGPENPTHDIMTPNYPFDYPLNTECNW LFVAPEGSEVMLNFNDFDVEGDDFDCFDYVTIKRGLPGIPGVSYCGTEFKAAIKSIDRYLMITFTSGSEITYTGFHATGRLLDVADLCYNPDDFGRTYNGIHNYAEDFTECLPW VEMAHCDTNLFNTEDAFDNLDGNYCRNPGARYDSPWCYTEKENCTIKYCDVCQERTIYDNFDDCEELIAEHPNFCLDGDHHHYGCLATCIEYGHITIPEKSNRAQDVSCPVPDDIVDADPIVHDVTRYNVGDSLAYVCSNGTATKMRRCLTDGTWTDIEYVCGGCGDEMKSDTRGTCYAYVAEEVRFVEAEEYCIEKDWDVASAQTEDAFNFLVQYRSDMGSDGKQVW LDLYEDPVGVW SW GDESLVQDGFTNWRSTYPVSGPIETDGNNW YRCATMVGTGQSSVWNQVACDNKW YKAHYICQTMESEREICADRKSSCTRNLIDIPLLCSQYQSYAHEVCPYTCGICDVENADSCTVTADFVVGLNLVSGGSTPFSLNPGESFTTQCPAGQICTQNCQHFSRACRRSGEVTGATPVCQDADSVPTLVNNIERIPRLDTSEARRIYQGDNSYMNITRSGEITKWMTLCENDGIVHLMAFKYW SQDRYFKVMGVNQVECKAGRRMTW EIPEGERIQVDPSMNIGLMDWNGGCIPLYECFASEYPDLNKIYHTMAESQDEVALNKLVWMRAGPTCYFFSLNAEISPVGYIAPATTQLTTPSTTTEEPEPEPEATSTSSEDTSESTDMTTSSGDVSTSSTSTSAGNTESSSTGASRSSPSTDAFVSEDTTSSVW QRTTTHSVVITTPRRTINEDFIKSFIKKKKKKRKWWKNRGKKTDKTRKRKNKRRKKAQKGR* Lottia Protein Model: jg i_Lotgi1_173484_fgenesh2_pg.C_sca_161000006 NR Annotation: no hit Sequence: MRLHYLVTVLVVLILIDVGEGQDNISKCLELPSVKEIVTRYICPKPDPCATVDCNCNGKCVDGKCQCKNGFFGEFCTALTSLIPKVAGFVSNNKSLISSEAQTIGSIAKAGVSKKSKELKELQQIRKLRNRRLEEKKGKDFKDIQKSLGSLLGFPWAKYPGEKHLQDHNYTGPGTRLDLRLDENDKPKPGGNETFTFDFISIMLGKKQFE* Lottia Protein Model: jgi_Lotgi1_176704_fgenesh2_pg.C_sca_2718000001 NR Annotation: PREDICTED: similar to rCG46800 [Nasonia vitripennis] NR E value: 6.00E-09 Sequence: MLTIRVLGVFAVIVLAFVLVESDVELCEDDVNVCHGELPANAMCSGENNKTKRCQCKKGYKMSLDIDCTSNGSVFSCQDIDECATNNGSCEYKCQNEPGSYNCSCPEGSELQGNGLNCGPSGP Lottia Protein Model: jgi_Lotgi1_177026_fgenesh2_pg.C_sca_12012000001 NR Annotation: no hit Sequence: MMTKMLTIIFSVLIVLCSSSPECGPNYFEGDKWFDGCNW CTCTDNKEKCTNATCTEYQDELDNSPCQKLGESWRDGCYHCRCLPDGIRCRANKRCVHALKSDDLNCPVDGLCVCGLDGVPSCNEQIMSRFSFKNVPELGPDTSSDKKLILSSKGENTLRLNPDEAETDLGDKDLHLIPTDDKLCHLGPRW YGGGLRCYCDPQNGVACGNPRFIARKVDGFKIRSKKCRVXKIWEERCRICTCKKSGLSLCRIDRECTKNKNIRHSVKSIV Lottia Protein Model: jgi_Lotgi1_197084_estExt_Genewise1.C_sca_820083 NR Annotation: glucose-regulated protein 94 [Crassostre NR E value: 0 Sequence: MKRFIVLGILGFLFIAGAGLLNVQAEEVVTEPDVVEDIGKSREASRTDDEAVQREEEAIKLDGLNVAQMKELREKAEKFAFEAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRFVSLTDKSAMAATDELSIKIKADKASHVIQITDTGIGMTKDDLVKNLGTIAKSGTSDFLSKLGESKDQAQMSDLIGQFGVGFYSAFLVADRIVVTSKHNDDDQYVW ESDSSSFSVAKDPRGNTLGRGTMITLHLKEEAHDYLEESTIRSLVKKYSQFINFDIFLWASKTEEVEEPIEEEEEEKKEEEKKEDEDAEVEEEKEEKPKTKKVSKTIWDW ELINNVKPIWTRKPSEITDDEYNDFYKSISSDYEKPMTKIHFTAEGEVTFKSILYVPNSSPSDTFQNYGKQMEQIKMYVRRVFITDDFEDMMPKYLSFIRGVVDSDDLPLNVSRETLQQHKLLKVIKKKLVRKTLDMIKKIDKDTYEKFW KEFSTNIKLGVIEDTTNRTRLAKLLRFSSSNSDTELTSLAEYLERMKEKQEHIYFVAGHNRAEVEKSPFVERLLKKGYEVLYLTEPVDEY

194

CIQSLPEFESKKFQNVAKEGLKLDDSEKAKERKEATEKEFEPLITWLKDSALNDKIEKATISERLASSPCALVASQYGWSGNMERIMQAQAYAKAKDPSQEFYATQKKTLEINPRHPLIKELKKRVEANKEDETAKDLAVVMFETATVRSGYRITDTAGFAQRVENMLRLSLEVPLDAKIEEEPEEEPEEAEEEKTEEVEAEAEEEAEEKKSEHEEL* Lottia Protein Model: jg i_Lotgi1_201804_estExt_Genewise1.C_sca_1950010 NR Annotation: DICTED: similar to putative porin precursor [St rongylocentrotus purpuratus] ref|XP_001192936.1| PREDICTED: similar to putative porin precursor [Strongylocentrotus purpuratus] NR E value: 8.00E-19 Sequence: MRLLIFTLSLLVAVVRSVDASQCPEPPSGFGPCVITDANCLTDSECSNGQICCPHLCGKICKDKVSLTVVKPGTCPSNAAISCFVGSVADTCSSDSDCPGNQKCCNLCGKNCSPPQGSAPQLPPIGLPAPPPVCPPVCAIACEYGNVLDQNGCPTCSCKKTPCKGEAPLTLDRNGNTINCGRGGVRCPLNSECKIHPADAYALCCPIEPDDPKPGVCPKLPNPLPRCLIFQNDCKQDSECPGDQKCCSVTCGIGCIDPVKLCPVVDCLQYCEFGYRLDSNGCQTCQCKKSPCEDEAPMTLDSTGNTINCGRGGVRCPSNSECKIHPADRYAVCCPKVCPPVCEIYCEFGNVLDENGCKTCKCKKSPCEDGAPMTLDSNGNTINCGRGGVRCPLNSECKIHPADRYAVCCPKVCPPVCAIYCQYGNVLDENGCATCTCKKTPCKGEAPLTLDRNGNTINCGRGGVRCPLNSDCKIHPADAYAVCCPKEPSPVCKFGSCPVPPSSFLLRCASIDRCKYDSECEGTKKCCKHPCGRQCRDIVKSKPGVCPKKADPTLVLCKRRGDDCYEDATCPGSQKCCQGACGNACMDPRLTSTDHAGTCPALDPKIQSLILCASLDTECELDSDCKVDQKCCKDYCGIKCVKSVPKPIIKKGTCPRLRYRCRRKRRDFCRSDGDCPGDRKCCRLPCGYKCRRPKRIPIIRKECPAVNTHPIYKLFCSPPLNPPCQVDRDCPQGKICCPGVCANRCLKAPTKPVCDPNPCGDAQIKCQYGQTKDENGCLLCQCKSYDFKMS* Lottia Protein Model: jg i_Lotgi1_204770_estExt_fgenesh2_kg.C_sca_820009 NR Annotation: unnamed protein p roduct [Tetraodon nigroviridis] NR E value: 3.00E-35 Sequence: MAATTHKMFLVACLVFLPIFASADTCLGPSVSAETYTTSEVTVSTETVFIAQFTLTCKNGLQNVNLYAEVAGRMIPAVKTSKPNEYQISITDEYKALRSGTYEAKFYDEESFSALKKAKRNNEDSNSVTPLFSINISHSGLWQGSYIQSEFVAACVAILVWW LAFSAKSKLSP*

195

Object 2-9. Predicted secreted signaling molecules found in Melibe leonine


Lottia Protein Model: jg i_Lotgi1_159314_fgenesh2_pg.C_sca_20000054 NR Annotation: Buccalin p recursor [Contains: Buccalin-D; Buccalin-E; Buccalin -F; Buccalin-G; Buccalin-H; Buccalin-A; Buccalin-I; Buccalin-J; Buccalin-K; Buccalin-L; Buccalin-B (BUCb); Buccalin-M; Buccalin gene-predicted acidic peptide A (BGPAP A); Buccalin-N; Buccalin-O; Buccalin -P; Buccalin-Q; Buccalin-R; Buccalin-C; Buccalin-S; Buccalin gene-predicted acid ic peptide B (BGPAP B)] gb|AAB27696.2| buccalin precursor [Aplysia californica] NR E value: 3.00E-66 Sequence: MAARKHELVLVLTSVLCFVSSIVGDPNVPSDSQDNSALTQDDFAKRGMDKFGFAGGVGKRGLDKFGFTGQLGKRDMDSFGFAGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFTGQLGKRGLDQYGFAGQLGKRGLDQYGFTGQLGKRGLDQYGFAGQLGKRGLDHYGFAGQLGKRGLDQYGFAGQLGKRGFDQFGFAGQLGKRGLDHYGFAGQLGKRGLDQLGFTGQLGKRQMDIFGYRGQLGKRQSIDKYSFLGAGIGKRSVKNTAGIKKDDA* Lottia Protein Model: jg i_Lotgi1_167943_fgenesh2_pg.C_sca_71000005 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO34356.1| p redicted protein [Nematostella vectensis] (Fulicin-like) NR E value: 1.00E-21 Sequence: MKSILVTSVVVVAGTLLTLVQALGADKFAEPATQKHSKIPTAALSHSGDTLLNTDAHQTNPSNIWKSKSKFDLDALPVVNRQDVQSHYAQHRRSYDTPMFLGKRDLKNLSLRFKRATDLEDLDATEFSDDFDKRGVDIPQFIGRRRYDTPQFIGRRGYGVPAFVGKRAFRAPAFIGKKAFGAPAFIGKKAFDAPSFVGKRKLTAPSFIGKRGLDVPAFVGRRAYAPPQFIGRRGMDVPAFVGKREFQTPMFLGKRAFSAPSFVGKRGLDAPTFIGKRKEESFRDLLATLQAYREYRRLMQTDKRFDAPLFVGKRSDYENDNLAAETLSRRFQCIPQW VFRMRLNVVDLNMSFIGGYKQNYFSLNILDDVQTKILLPLDDLHKVDSSKMDVPTVQLWDADMPRPNGARFLKLDPAHALGFLGQEASPVSKRYLEFVGKRDNDKRYAEFVGKRAGFGEKRYSRGSLSEYLSKRLRIRRPEFVGKRSNQKRYLEFIGR* Lottia Protein Model: jgi_Lotgi1_171718_fgenesh2_pg.C_sca_119000006 NR Annotation: PREDICTED: similar to orphan G protein-coupled receptor HG38 [Gallus gallus] NR E value: 1.00E-15 Sequence: MAPIYLTFIASLLLASSTFCEQLCLEGCKCEDHPSEKLGPHLMIDCSNLGSSSVPRDLNKFQNYGRPMHLKLSKNIIARVSKADFPENLPIIALDFNQNRGVMITDDAFMNIKGLRYLGLNDISMPFTDTMKYLKGMTKLRYLYLNKNYQFGEGLVPVSLFSGLELKLKDLSLHDCYLKGIENGALANVVITGSLDIGANQLDHIPEEVKTLKNLTKLDVSQNSIKSIPSNTFEGMSKLRTLSLSNNYINGQSFKTDSFNGLKLKELRLEKSELNTIPSAALSSLSSLKILKLNNSPIKTIPKHSFNGSYCLEVLDLTNVLVEIENTHLDGLESCLQKLYLNNMQIREIPKFFKDFKVLNYVSLSGNSISEIKMDDLVGTTITELDLTENPLHRIEKGSFEEFPHAISLIISDTRLSNLSFVRDYEYGTIKTIKLGNVLPMCDCELAFAEYRVLEIHGNCNFNNTKNVAISTDEFMSVTSHCWPDGKMPQKKDVWAVVGDNSAGTFSTSILSYFCVFLFFKVLNIV*

Predicted molecules found in Melibe leonina with unknown function or predicted to be related to the secretory pathway

Lottia Protein Model: jg i_Lotgi1_151060_fgenesh2_kg.C_sca_80000004 NR Annotation: LOC431836 protein [Xenopus laevis] NR E value: 1.00E-140

196

Sequence: MDFARLFQSLCICLLTLDIQLFGLTSGANPEVEQHLEMGKKLLAAGQLADALSHYHAAVEGDPDNYMTYFKRATVFLALGKSRSALPDLAKVVELRPDFTAARIQRGSVLLKQGKYKEAKNDFETVLKTSPNNEEAKRNIDKIETIPPDIELAYRLVEEGNYGDAIQVLGGIIDTCPWDPTFHEIRSECYEAQGDLFKAASDIRQSTKLRADNTRGYFKLSSLHYEMGEADESLVQIRECLKLDPDNKQCFPHYKKVKKLVKLMTSAQESINNEEYDKCVKKAEEMLKVESLVHHYVSRAGQHKCKCYSKGGKVKEALAECTHLLKIDPNNLDALIDRAEAHIFNEDYEAAINDYQEASNIDGENRRVKEGLNKANKLLKQSKKRDYYKILGVKRNAKKKAIMKAYRKLAVLW HPDKFTTDEEKEKAQKKFLDIAAAKEVLTDPEKRAQFDNGEDPLDPEEQSGHGGNPFQQGFNPFGQGFSGGSFKFHFN* Lottia Protein Model: jg i_Lotgi1_153437_fgenesh2_pg.C_sca_3000340 NR Annotation: hypothetical protein PB105991.00.0 [Plas modium berghei strain ANKA] emb|CAI04701.1| conserved hypothetical protein [Plas modium berghei] NR E value: 8.00E-09 Sequence: MKTMVAIIGVIIVSCVLHTSGLPLFKNILNERSVEYGGESGNGFKNERAVGYGGESGNGFLNERSVEYGGESGNGFQNERAVGYGGESGNGFLNERSVEYGGESGNGFQNERAVEYGGESGNGFLNERSVEYGGESGNGFQNERAVGYGGESGNGFLNERSVEYGGESGNSFLNKRAVEYGGESGNGFLNERAIEYGGESGNGFLNERAVEYGGESGNGFLNERAIEYGGESGNGFQNKRAVEYGGESGNGFLNERAVEYGGESGNGFLNERSVQNSGKSGNGFLNERSVQQGGKSGNGFLNERSVEYGGESGNSFLNKRAVEYGGESGNGFLNERAVEYGGESGNGFLNERSVQYGGESGNGFLNERSVQYGGESGNGFLNERSVEYGGESGNGFLNERSVEYGGESGNGFLNERSVEYGGEAGNGLK* Lottia Protein Model: jg i_Lotgi1_159188_fgenesh2_pg.C_sca_19000225 NR Annotation: predicted protein [ Nematostella vectensis] gb|EDO44886.1| predicted protein [Nematostella vectensis] NR E value: 2e-07 Sequence: MRGPTWSVILLLINLIVTLQACDDVTEFQCADNNQCISKTDTCNTINDCDDGSDENNCACLEKLGMEDGTILDSSLIASSSKTGSSPSYARASNDKGNW CPKSDDSKPFFKVKFDTPRAISAIVFTLKQSVVDPLNYLSTWTLKAVLPGSDEEVTLYEGLNAFSNNSLYRSLFFPSLVTDTFIIAPTTW QNEPCLSFELYGCNIDNLCPTGCQNNGKCSGNDICACADGYFGTKCQFQKTTIPFSSYIFKSLESGSLVTSSETFTVRTSTTIRTSKIIEIQGRKVLSLPVADYFTAPKDGKFGCLSKLGSGGCKSFYLA LGCELEELKDTVQLFTTGSDSTYIGAEATLKSDNLVVTFRTSELIWTLNVRKTNMFSSSIKYVLEFDWDISTGIRIYVNKKLIGQQRQATRTTQVLRTNTLATSGVEFFKSKSVNVKLFSLKSSSVNRISLIKTSLISLTPTEECDQEKEHKCANSDKCIDKSNVCDSVEDCDDGSDEQNCPTTTCDVKVYKKLVTKSASLSLDLLEVDDAQRVAKCTLPVISYVVDGILLDFLSKNQYGDQLSATNDLGANQNHNNNNKDDNNNNKKSSQDEFKEDVFNWADEPWTPCDDVEVPDWARMDDPTFDNFNSELIWAEDPWTQLPLVGLPSWMLLPGIDYPVEAKDPVVYTPVNDEDDAAFLASGIDIVNWSDEPYFQLPLDVPRWALLPGIDYPVRHGYNSPYWTYKLNQYNSSRREFGKKMFARQFPTLREERVLYNNNGQSKLCIPGNFQRRNHRF* Lottia Protein Model: jg i_Lotgi1_163475_fgenesh2_pg.C_sca_40000029 NR Annotation: Myc homolog [Crassostrea virginica] NR E value: 8.00E-08 Sequence: MDLSYLYYGMFWFSCLLVSTVSAEFSYEEVTYRIPPTPTGFYDQNCTDKISDCEEFGIASCFKPYESWARENCAKTCEFCQGKPEKPPTCTDVEKGCDLYTTSICTDPSFISWAKAKCRRFCRMCPEQVLAELDLMTTTLTPVDCFDKVVCERYGKKACTGDFASWATDNCQKYCGFCSTGVTKSKRLCVDKLPNCPQYDSRICTDKKYYSWTDENCRSTCKFCQDKLVPFLIPTPQAVRTPKPPTT* Lottia Protein Model: jgi_Lotgi1_164905_fgenesh2_pg.C_sca_48000037 NR Annotation: no hit Sequence:

197

MMTKMLTIIFSVLIVLCSSSPECGPNYFEGDKWFDGCNW CTCTDNKEKCTNATCTEYQDELDNSPCQKLGESWRDGCYHCRCLPDGIRCRANKRCVHALKSDDLNCPVDGLCVCGLDGVPSCNEQIMSRFSFKNVPELGPDTSSDKKLILSSKGENTLRLNPDEAETDLGDKDLHLIPTDDKLCHLGPRW YGGGLRCYCDPQNGVACGNPRFIARKVDGFKIRSKKCRVGKIWEERCRICTCKKSGLSLCRIDRECTKNKNIRHSVKSIVVAGNAECRVGLRW KDGCKRCRCNKRGKIICKTRLCNLSDDGKKLSNKDKTHRQTSTGGLISKSTPRFQLPEKPRFRMPNLSLYNNRFQRKKRPSIKTVNCNGFKPGEKYWDKCNLCHCTMAGAPMCSSKLC* Lottia Protein Model: jgi_Lotgi1_166436_fgenesh2_pg.C_sca_58000065 NR Annotation: PREDICTED: similar to MEGF6 [Strongylocentrotus purpuratus] ref|XP_001187830.1| PREDICTED: similar to M EGF6 [Strongylocentrotus purpuratus] NR E value: 1.00E-26 Sequence: MMKILEILLCLSFFRATVQQFDCSVCKVCDNSTCIECIEGRYGISCGSFCSLKCSGRCRQSDGYCTSGCDEGYWGPGGLCDQTCSNCNPGGCVMNSGLCIAIGCRTGW YGTTCNNKCNSNCDGNCNQSNGNCNQCIPGKYGTDCQLDCSNCKDTCNKNNGVCNNGCKEGLW GTSCTKTCPNCQSSGCDIDNGNCNNPGCRIGW YGDSCNDKCNERCNDGCKQDTGYCNKCNKGVYGNVCDKNCNKGCLDGCNIDGSCKCKSTYYGTNCSKQCSSNCVDSLCNEISVGYNSCIRGCVAGYKGLYCDKQCPSSCPICNRFDGSCTECSKGFYGTECQLKCPEQCDKCKIKEGTCESCNPKYHGPVCSKRCSDKCLNGECNGVGGRCIDCIPGTFGDNCERSCSNGCIGDTSSTTCDRNGSCISGCINRIYGEKCNLNCSKNCYGSCNRSNGYCNSCLSGYFSDNCNQKCSIHCNGDCDRSTGNCGKCNEGYFGDNCNQNCSINCNGGCQKSSGSCLECNAGYYGDYCKQQCNDKCINNECLRNGQCRFGCMNGKTGSQCDGNCNSNCTICLQTDGNICKECKPGLYGISCDKLCNNTCKKVNSKPACNITTGKCTTGCIDGYKGYYCQDTCLDNCISCNNNQCTSCNTGW YGNQCQTKCNSNCINRCDQATGDCLDGCIDGKSGKHCKQEIG* Lottia Protein Model: jgi_Lotgi1_197084_estExt_Genewise1.C_sca_820083 NR Annotation: glucose-regulated protein 94 [Crassostre NR E value: 0 Sequence: MKRFIVLGILGFLFIAGAGLLNVQAEEVVTEPDVVEDIGKSREASRTDDEAVQREEEAIKLDGLNVAQMKELREKAEKFAFEAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRFVSLTDKSAMAATDELSIKIKADKASHVIQITDTGIGMTKDDLVKNLGTIAKSGTSDFLSKLGESKDQAQMSDLIGQFGVGFYSAFLVADRIVVTSKHNDDDQYVW ESDSSSFSVAKDPRGNTLGRGTMITLHLKEEAHDYLEESTIRSLVKKYSQFINFDIFLWASKTEEVEEPIEEEEEEKKEEEKKEDEDAEVEEEKEEKPKTKKVSKTIWDW ELINNVKPIWTRKPSEITDDEYNDFYKSISSDYEKPMTKIHFTAEGEVTFKSILYVPNSSPSDTFQNYGKQMEQIKMYVRRVFITDDFEDMMPKYLSFIRGVVDSDDLPLNVSRETLQQHKLLKVIKKKLVRKTLDMIKKIDKDTYEKFW KEFSTNIKLGVIEDTTNRTRLAKLLRFSSSNSDTELTSLAEYLERMKEKQEHIYFVAGHNRAEVEKSPFVERLLKKGYEVLYLTEPVDEYCIQSLPEFESKKFQNVAKEGLKLDDSEKAKERKEATEKEFEPLITWLKDSALNDKIEKATISERLASSPCALVASQYGWSGNMERIMQAQAYAKAKDPSQEFYATQKKTLEINPRHPLIKELKKRVEANKEDETAKDLAVVMFETATVRSGYRITDTAGFAQRVENMLRLSLEVPLDAKIEEEPEEEPEEAEEEKTEEVEAEAEEEAEEKKSEHEEL* Lottia Protein Model: jg i_Lotgi1_204770_estExt_fgenesh2_kg.C_sca_820009 NR Annotation: unnamed protein p roduct [Tetraodon nigroviridis] NR E value: 3.00E-35 Sequence: MAATTHKMFLVACLVFLPIFASADTCLGPSVSAETYTTSEVTVSTETVFIAQFTLTCKNGLQNVNLYAEVAGRMIPAVKTSKPNEYQISITDEYKALRSGTYEAKFYDEESFSALKKAKRNNEDSNSVTPLFSINISHSGLWQGSYIQSEFVAACVAILVWW LAFSAKSKLSP* Lottia Protein Model: jgi_Lotgi1_202874_estExt_Genewise1.C_sca_21760001 NR Annotation: pred icted protein [Nematostella vectensis] gb|EDO37140.1| predicted protein [Nematostella vectensis] NR E value: 2.00E-54

198

Sequence: MMKLFVILALLAGQSLADIYLHNPRGSNDRLNEKSAQRANANRLFDSQNNNRGGYNVGDVTSAPHGKDASKQYKMAYFQSEADAETILTVEW YNQHGCGGNEDDNPQKQNCRLVLQYMCQPEGTTEDVLRNGVVTNTQDYNRPPNSNYNLANRNSRKNNNVKADRGLQESW DW YEECFVRERNKGLFTADQNLRGNNGLGYSSAIYTR Lottia Protein Model: jg i_Lotgi1_203811_estExt_fgenesh2_kg.C_sca_240005 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO37140.1| p redicted protein [Nematostella vectensis] NR E value: 0 Sequence: MMKLFVILALLAGQSLADIYLHNPRGSNDRLNEKSAQRANANRLFDSQNNNRGGYNVGDVTSAPHGKDASKQYKMAYFQSEADAETILTVEW YNQHGCGGNEDDNPQKQNCRLVLQYMCQPEGTTEDVLRNGVVTNTQDYNRPPNSNYNLANRNSRKNNNVKADRGLQESW DW YEECFVRERNKGLFTADQNLRGNNGLGYSSAIYTRQNPNGNRRGYECPEERDYFPYW HPTQWKDIAILAENETLCNPLIAESFNNKPYGACLQKFPNSQVRKPSRYNNKEECEENGLDW VAFHNYLEKDKTASTQAACQAKSTNNIEYTWAIPYDSTTFTAECLVKVPSPQCQEAPWSRSNHLGNGIGGEMNTVQWTLPHFPSGQVQNCVMRMRYNISTDDYDPFNTDSEFNGADNSPVTNNPYVDIGVGRGPLRLAINTAQFGRVFQDRTHIFQLKPRPDDIKDFRIFNVNVRGKRGNIVQVYPAVEYDFTPTDLEVTENDLVHFQWTGSNTHNNGRPGGDGQTGDAGEGKSGSDRHNILQMADRNENFPLPFEKTNMWDNAEIKWIYHGKNDVSARDLALNMASSGYYKCVSATDCPAESFKDFLVDTKDKMQVTLDNTPASYPGVVLKFKQGKYHYMCTRNNNFTNRSQKATITVAA* Lottia Protein Model: jg i_Lotgi1_67930_gw1.39.124.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO32094.1| predicted protein [Nematostella vectensis] NR E value: 1.00E-10 Sequence: VGHFVLSCCMW RAVFSCCMWRVVFSCCMWRAVFSCCMWRAVFSCCMW RAIFSCCMW RAVFSCCMWRAVFSCCMWRAVFSCCMWRAVFSCCMW RAVFSCCMWQHVASCIQLLHVASCIQLLHVASCTQLKHVASCIQLLHVASCTQLKHVASCIQLLHVASCIQLLHVASCI

199

Object 2-10. Predicted secreted signaling molecules found in Clione limacine.


Lottia Protein Model: jg i_Lotgi1_163440_fgenesh2_pg.C_sca_39000174 NR Annotation: No match found NR E value: Comment: Bel-2 like protein Sequence: MDLYGVVW LLAILQEVTSCFLTEKLPSLEGKEWFTKIRSGGSNDVIAYFDRNSVTFNDVKNTTNGETKW RCFMKIRGRYFLKNVRQGGGYLYKCMGIIVRSESAIQLEWSHVSRLADPALCAEENINLDPW LLISYKTVVNDFTSCPVSGGFDIKHAGYYSRDVRDSRGYDIGCNLMNIPMRLEFDCIAGEGAIFNFRSKNCVPDIHFNIYQNTICVAKWSNKRHHFVLLRTKTGLEFW CARFPVGIENQNETELYLYSDVACLEGEYAEEFVKFMKFDLQRVMYTTICADEYPQCKEGTCNVFSKLECQKTCGLCNPHRPPGICTFPKRMQGTYLLHSKHGNQNVTLEGRTLNIENVGQFDCIVFEDSPLRSTKTYTTMSIFQNGCRPRYTCVRLKRLGPSALRYSLAQNLVW PIKKERIGANICNEDNFRADGDPIRDTFRSYKKTGKPVIKIHPKPRFINCGMNTSYTIRAELPDGSICHGGFYQHCKNETKLRFDFHTCNSFIKPKEDFNCAGIFRTNYW ERAVLIQNVDNLNDIRCLIFNSLRPYQALIVVADECDKMASGLVDSNIRIPIMRLHIRSDVYPCKHIQLPLKDDVITNVVTTTEPIKMNVTLKESKNVNSFSVDLELSSRNSSGSRPFENKLNSLLLLFLCYVVFVKNL*

Predicted molecules found in Clione limacina with unknown function or predicted to be related to the secretory pathway

Lottia Protein Model: jg i_Lotgi1_118077_e_gw1.28.72.1 NR Annotation: LOC100124858 protein [Xenopus tropicalis] NR E value: 1.00E-108 Sequence: MGSTLNILVILGCILVPFSFSNFLEW GPDLEGPW CATRPIEQCCPGRDDECTVPILGTKCYCDIFCNETAEDCCPDFW NLCLGVTRPTPWPLTTTTSRVPINILCNVYW FKCTKLHFSSHWECSNDDCLLEADHITQINNGPYSWVASNYSDFW GLTLEDGVKYRLGTFPLGSNVVQMTPLRVKLTDVLPESFDARTKWPSYIKPIRDQGNCGASWAFSTTAVASDRLAIESLGEIKDELSAQHMLSCNVRKQKGCEGGNLDRAWWFLRKTGVVTEECYPYES GDTTDKGDCLVSRRKSNTTCPSRILYKEKRRYKATPPYRIAPKEREIMKEIMDNGPVQATFMVKSDFFMYKNGVYRYSNISFDEEPAVYHSVRIIGW GVERTIYGDILKYWICANSW GTQW GENGYFRIIRGRDECEIESYIVGVW GQISGDASLRTLLTRGRRRRLFAERSLRNLRVNSMAKGRKSRSRQVGRKKVKRGRKSREERRRLRQEHRNKRKLRRQSRKQDSDSQNTV* Lottia Protein Model: jgi_Lotgi1_118302_e_gw1.28.365.1 NR Annotation: No match found NR E value: Sequence: CVMLYMLCKCSSLDCVKLYVLCECSICHCVKLYVLCECSICHCVKLYVLCECSICHCVKLYVLCECSICHCVKLYCSICHCVKLYVICECSICHCVKLYMFCECNICHCVKLYVLCEV* Lottia Protein Model: jg i_Lotgi1_143169_e_gw1.194.72.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO36917.1| p redicted protein [Nematostella vectensis] NR E value: 1.00E-28 Sequence:

200

MVKVCSIPLTVVKVCSIPLTMVKVSSIPLTISKVCSIPLTMVKVSSIPLTMVKVCSIPLTMVKVCSIPLTIVKVSSIPLTIVKVCSIPLTMVKVSSIPLTIIKVCSIPLTVVKVCSIPLTVVKVCSIPLTMVKVSSIPLTMVKVCSIPLTVVKVSSIPLTMVKVCSIPLTVVKVCS Lottia Protein Model: jgi_Lotgi1_166736_fgenesh2_pg.C_sca_60000104 NR Annotation: hypothetical protein LOC500991 [Rattus norvegicus] sp|Q5XI89|FA55D_RAT Protein FAM55D precursor gb|AAH83798.1| Hypothetical protein LOC500991 [Rattus norvegicus] NR E Value: 3.00E-25 Sequence: MALTKKLVLICCLIIATVFGLKIMHTIKYQINMRPNIEPIPFYSQNTPKRKLMFPERQSPSLEDYNMKHDDVRSEDIFNKFLSENVVYVKDTTADDYFIEGEKELLEEGEIKDLRDLPSSNYSIISTKNTVFNQYEAVTVKIQLYNGFGQLLKRGGDDVRVW ITGENFQAGGEVYDLHNGTYVAEVKAFWTGKSKIHVSIAYSREELRILLRTRRESKVLRYSVGRFLRGGDTFMTLCSPFHDIPLYPRVCNFTNFNLNPYYCGQPNSPKVACSDLRILHTINYSKQRMNNLESKILSRQNRVNELKSKSITINIGTVVNLPYCSSYKSRFTWTNTAPLGYFNNNTWHPSFCQGQITRQRLSRCIRDLHVYLVGDSTVRQIFQSFLRLTKCTNTTFIGNRPHDRVQRYCDVHDSNFRVEYGIHGLPYSMVRTPDLLNNTKAIGFHLDGLQWSQNKDKMFVMVHLYAHFLFFHTSLFEARVRSLKVPLTRLLNRYPKIKIFIKGPHAFTGADFKPIVKSDYFGKVYRSILLKELFEFRDRVYYLDYWDMSVSSGNRLLHPVNSFVDEMVKFFLNLACDK* Lottia Protein Model: jgi_Lotgi1_173456_fgenesh2_pg.C_sca_159000027 NR Annotation: No match found NR E value: Sequence: MWSGVW CGVVYGVMWSGVW CGVWSGVERSVVW RGVWSDVDGVMWKECGVVYGVVW CMKWCGVWSDVERSVVWCMKWCGVW SDVERSVVW CMEW CGVWSGV Lottia Protein Model: jgi_Lotgi1_175543_fgenesh2_pg.C_sca_302000002 NR Annotation: No match found NR E value: Sequence: MNTMFLLVVFLLIPATSAFDSTQTNRTIVNSTITTKINGSSIGLGTCSEFTPQSCSGVCGQFFELW CSCDEYCQVYRRCCFDYEAVCKEESEKSKQDHENLMNVTVECDSNMSGVHVPQQRISPASG* Lottia Protein Model: jg i_Lotgi1_67930_gw1.39.124.1 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO32094.1| predicted protein [Nematostella vectensis] NR E value: 1.00E-10 Sequence: VGHFVLSCCMW RAVFSCCMWRVVFSCCMWRAVFSCCMWRAVFSCCMW RAIFSCCMW RAVFSCCMWRAVFSCCMWRAVFSCCMWRAVFSCCMW RAVFSCCMWQHVASCIQLLHVASCIQLLHVASCTQLKHVASCIQLLHVASCTQLKHVASCIQLLHVASCIQLLHVASCI Lottia Protein Model: jgi_Lotgi1_98330_gw1.10.599.1 NR Annotation: hypothetical protein NEMVEDRAFT_v1g147894 [Nematostella vectensis] gb|EDO28414.1| predicted protein [Nematostella vectensis] NR E value: 5.00E-06 Sequence: TVCSLGTHCLSITVCSLGTHCLSITVCSLGTHCLSITVFCSLGTHCLSITVCSLGTHCLSITVCSLGTH CLSITVCSLGTHC

201

Object 2-11. Predicted secreted signaling molecules found in Octopus vulgaris.


Lottia Protein Model: jg i_Lotgi1_121665_e_gw1.37.7.1 NR Annotation: hedgehog [Patella vulgata] NR E value: 1.00E-171 Sequence: MKLFFSFQSVCSWTFIFLFITSLTHACGPGRGSGSRRRPRKRTPLVFKQHVPNVSENTLGASGISDGKIRRNSEKFKNLVKNENPDIVFKNEEGDGSDYLMSRRCQDKLNSLAVSVMNNWKDVRLRVTEAWDDSPNSHAKDSLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDW VYYESRGHIHCSVKSDSSVAIKIGGCFPPTGSVQTLHGWKTMGQLTVGDKVLSINSAGQLEYSPVIAFIDRNDLEFEKYLTLHTEDDTDITLTSKHLIYASGTNSSNFESYDVVYADDIMEGDHVLITSSEKGAISPTRVVTISEKTLQGVYAPLTVNGNIVVDGVVVSCYAVVSDANLAHAVFAPMRGLHYLSQYVPW FLHSTQQENAPQNGVHW YAKMLYNIGSTFLNEKTLYIA* Lottia Protein Model: jg i_Lotgi1_168377_fgenesh2_pg.C_sca_74000087 NR Annotation: TBL-1 NR E value: 2.00E-07 Sequence: MLPHITQCVILFITFLCDISVLLPSRDGGRGLTYAQLVPHRTSNDVYMDPCKAAGFMGDIALSEEEFKRERKYLQQMNNTMFELQVEDPRTTYIGRKPSKYSGQPENTLKKLPMSELDVKKKKYLRKILKEKKQNLKSLCKKGFQTVVYTRQVPVGFKFISPFLYLLDLNIL* Lottia Protein Model: jgi_Lotgi1_168619_fgenesh2_pg.C_sca_77000042 NR Annotation: crossveinless [Saccoglossus kowalevskii] (a new family of twisted-gastrulation-like modulators of bmp signaling) NR E value: 1.00E-148 Sequence: MYYTELSILAKCIALCILTCFPILIQAQLTGTVTRCYNEGGEVKIQGITTDPCISCYCMDGYVQCDKKKCDNLDGCHAILFDGPKRCCDTCKGCRLNGVKHESGKSWFDKRDRCTSYSCKAGVITKSKVQCHVPCSNPIKRKRQCCPSCKGCHFDGADRKNGEKFALASDPCVECKCRKGSVTCMKRACPVLNCPDEVIYQPKGECCPKCKGMRKIFDFPGACYFAKRVYVDGFSFEPKSETRCTCTKGTVICEKATCPPVACPVEERVQKGSCQVCEPKRNCLYDGKIHMHRSKWQPRMCTQCSCQNGVTYCQRERCNNSLSCPNGYKLQFQPGECCPKCVEHDAVCRVFGDPHYRTFDGKMYNFQGTCKYMLSQDCQGKDFTIKVKNGVRLSSGFAWTQMVVVLMGDTRISFRQNLLIKINRRRVQLPYTIPGKFSIRREGHSVTFRADIGLKVVW DGDSFLEVTASRKYKNRLCGLCGNYNGLETDDLIGRKGKNYLRGEEFGNSW RIGSKKACKTQPKVKNLQSICDKDFKAKVRANKECSVLYSRAFSSCRRVVDVTPYVTSCVTDMCDCPHGKKCSCESIQAYAHECKRAGHKVKW EKVSNCKAPQKNCPKGAYYSLCAPACPKTCSSNKPDGSCSKKCSPGCVCQNGAVLYKNRCISPEKCPKT* Lottia Protein Model: jgi_Lotgi1_205842_estExt_fgenesh2_pm.C_sca_70027 NR Annotation: BMP2/4 [Patella vulgata] NR E value: 1.00E-159 Sequence: MIADFYRSVVLLLLVLVVNYTSSLSTNTLHPRKHGNEKVLAAVESNLLGLFGLKSRPVPGRRTPIPDYMLDLYKRLDSEPDFISPFAESKGKGIVEANTVRSFYHSDINFVKDCDERTCARIWFNISTIPFA EIMAASELRVFKDVYNHFKLTDANSLSGDRIKASLFKHRLEIHEIMRVSPDDSECISRLIDTKIVDTRNSSWESFDVHPAVLKWRRRPQYNYGLEVRIVSKSPWISTNSHVRLRRSAHMDEKNWQIQRPILVTYTHDGRNPNSRIKRASARNRRRKSRKRRRRKKKGNKNECKRHALYVDFGDVGW NDWIVAPTGYNAYFCRGECPFPMGQHLNSTHHAVMQTLVHSVNPSAVPKACCVPSELSAISMLYLDEW DKVVLKNYQDMVVEGCGCR* Lottia Protein Model: jgi_Lotgi1_188920_estExt_Genewise1.C_sca_260301

202

NR Annotation: predicted protein [Nematostella vectensis] gb|EDO37057.1| p redicted protein [Nematostella vectensis] (growth factor) NR E value: 1.00E-109 Sequence: MFLEVFNISATVLCILFLIGHRKCDTIQNSKHHAELMMDLLQPYKNYHGQRLKKSNRHIRECQEIKYGNVTHSKVKVKPDTNISYPLVEVKHLTKEIGSYYYKNPVTIRYQRVDFPLNTLSVLEPGEPGTCREGHGTRSITSKTALSGNCIVGVNGGFFNTTTGACLGNIVSNGRLVQDSQGLQNAHFGITEDGYIYVGYLSELDLITQQFKQLLGGVLW LIRDGEKYIDKSKQMECPDVEETGTIETFIEVQSARSA VGHDKDGRVIMVQVDGKTNARGVNLHEFTDILLELGVVNAINLDGGGSTTTVINHTLVNYPSDKCADGTFSCEREISTILCVHEPECQPNDCNQHGHCVLGKCVCDGYW VGSDCSSLSCPFSCSHHGTCTKDGCICDDGW YGINCSTPCKPGYYGSNCAHQCYCLNHGTCNSQTGHCVCSPGFTGKYCESLCEFGYYGDKCNNLCTCDNGCFCHHVTGSCNLS Lottia Protein Model: jgi_Lotgi1_208417_estExt_Genewise1Plus.C_sca_10710 NR Annotation: fasciclin- like protein NR E value: 5e-178 Sequence: MSRNLWIFLLILLSIIVIASSRRSRGRW YRGRSGRRSLADRLRDRGTKPSNHRPRQRHGW GRW GSGW GAGQDHRTRVEEELDDLFRRIKDRRYGFNFEDKFW LDFGWNSRNEPKPWWEGPNVCKDEKESIENTSISDTGPISRHFSFKNQVCDQSETSYKCTTKTGIFMKQKTVTEVYECCPGFTRKDEMFGCPTEFKLDGLVKTARNLGLTEFLRATESVSLTDDLEKGNFTVFAPVNKAFEDEKNILPELETILPKDMPSVVMVSKPLTDLVIADMSNVLLGHLVYDTLKTSGLNDEQLIETASPFDSLLRVNFFSRPEKLITVNCARILSADNQATNGIIHTVDRIIKPVTDSIVDIISQNPNLSYLKTALARADLVKALRGTGQYTLFAPTDAAFEKLEPSIRDRLIKGEKSCLEKVLKNHLLTSVICSTVIQGTVTTTNLLNKYLNLTRTEDDKLFVEGSQMVGRDIMATNGVIHVIEDVLVPDQALGLIDVAEKNNLTKFVELLKETGMFKGITKMENLTLFIPSNNAFEKLEEKVSLEELKKDKKKVEDIVMYHVVPEVVTCSRLHNNYKLSTENERNIRMNRYSTFPFRHHSIQTAQCAPIIKENVPACNGVINIVNEVLLPPVGNVVDNLASKKKFSTLVRLMKKSGLADMLQEEGPFTMFAPTNEAFDDLYEEDLEKIEDNPEVLKKVLQNHIVQEFVCCASISMVRSWFDVASVRTLSGVRFPVKERHGTIQFNHAVVTECDNTATNGVVHAIDKVTLSPERPFWKRDFYHW*

Predicted molecules found in Octopus vulgaris with unknown function or predicted to be related to the secretory pathway

Lottia Protein Model: jgi_Lotgi1_103075_e_gw1.1.1164.1 NR Annotation: PREDICTED: similar to CG31650-PC, isoform C [Apis mellifera] NR E value: 9.00E-50 Sequence: MNVYYNILILCLGVYCTLGEKEKVPNEPVDQKHTKDGEHNPHYDHEAVIGSKQLEHEFDELPPAEAIQRLENLVKHHDLNADNIITHDELKKW ILASYMSLDEEESDEKFQENDVDGNGNVSW RELVKKEFGYDLEDIEDFKKNTDKQEPDTKEFLLMLDEEEKRFMTADTDNNGNLEKQEFRAYYHPNEYPHMHGVEIERALRTHDKNKDNQVSKAEFIGGLLFLEDREVYLTEEENFNSFDLDGNGKLSQDEVRAW VIQDNNQAAEDEVLHLISQADDNKNNELTLAEILEHYDDFVGSATDYGHKLSDEL* Lottia Protein Model: jgi_Lotgi1_108564_e_gw1.8.40.1 NR Annotation: PREDICTED: hypothetical protein [St rongylocentrotus purpuratus] ref|XP_001179636.1| PREDICTED: hypothetical protein [Strongylocentrotus purpuratus] NR E value: 2.00E-86 Sequence: MYLSCYLPVILLFIEDLKQAVVAMMLRKDEVEEKNKSLKAMLDREMEISSTLRAEIEEMKISFKLSKDKEIAKNETLQKENELLKHQLRKYINAVQLLRTEGAKDDTQGITLEDPQPIIPPAKPSIDYSHEASEYEKKLIQVAEMHGELMEFNELLHRQINCKEAVIRNLKEELTDLRGPLPYDAQSSDDSLSGDLESSLISRCLINIWIPSAFLGGSKTDSHHVYQVYVRIRDEEW NVYRRYSKFLDVHTRLKKVYPLIEKFEFPPKKTIGSKDPKVVTARRKMLQSYLRKVINHLLEKNADLSSNVSKEKLIAVLPFFK*

203

Lottia Protein Model: jg i_Lotgi1_116422_e_gw1.24.410.1 NR Annotation: PREDICTED: similar to CG14764-PA [Apis mellifera] NR E value: 7.00E-58 Sequence: MKTKNFYILVLLISVTSVALW LVSYPSPTFRNIMSTTQNNIRKIPKHMQMSKSPDLEVDKKYLDLLGFDSESKPKNDIVVIGSALMPADTENTILFLKSAQEYVPNLKLVLFDMGLSKADKQTLTNCRNETKHCEIRKFNFDQYPSHISDFANKSYKPICIQLLLKEFGTVIWADTSELFASSDIDNTMKQAKTEGLVAWTIEDATSSLTHPKMFDYFKTDQKKYFFHHAVQTSHIILVNTDKIHENVMLPW VKCALVEECINPMGAQNSGCNYQRKPKFRYTGCHWYDMSALNVILGLAFDFDEAYSGKDKIFWTKFDQEKAKAENMTDGAVHKVSVPHRVYF* Lottia Protein Model: jg i_Lotgi1_118077_e_gw1.28.72.1 NR Annotation: LOC100124858 protein [Xenopus tropicalis] NR E value: 1.00E-108 Sequence: MGSTLNILVILGCILVPFSFSNFLEW GPDLEGPW CATRPIEQCCPGRDDECTVPILGTKCYCDIFCNETAEDCCPDFW NLCLGVTRPTPWPLTTTTSRVPINILCNVYW FKCTKLHFSSHWECSNDDCLLEADHITQINNGPYSWVASNYSDFW GLTLEDGVKYRLGTFPLGSNVVQMTPLRVKLTDVLPESFDARTKWPSYIKPIRDQGNCGASWAFSTTAVASDRLAIESLGEIKDELSAQHMLSCNVRKQKGCEGGNLDRAWWFLRKTGVVTEECYPYES GDTTDKGDCLVSRRKSNTTCPSRILYKEKRRYKATPPYRIAPKEREIMKEIMDNGPVQATFMVKSDFFMYKNGVYRYSNISFDEEPAVYHSVRIIGW GVERTIYGDILKYWICANSW GTQW GENGYFRIIRGRDECEIESYIVGVW GQISGDASLRTLLTRGRRRRLFAERSLRNLRVNSMAKGRKSRSRQVGRKKVKRGRKSREERRRLRQEHRNKRKLRRQSRKQDSDSQNTV* Lottia Protein Model: jgi_Lotgi1_118302_e_gw1.28.365.1 NR Annotation: No match found NR E value: Sequence: CVMLYMLCKCSSLDCVKLYVLCECSICHCVKLYVLCECSICHCVKLYVLCECSICHCVKLYVLCECSICHCVKLYCSICHCVKLYVICECSICHCVKLYMFCECNICHCVKLYVLCEV* Lottia Protein Model: jg i_Lotgi1_132441_e_gw1.79.137.1 NR Annotation: PREDICTED: similar to CG11081-PA [Nasonia vitripennis] NR E value: 4.00E-72 Sequence: MAWKIYVLFVCVLCLRFILAARENYIKATFEGAKDRGIFNKLVVNRETNELYLAAVNHLYKLSPNLEVIQEVVTGPHLDDPSCTSLTNLTCDPDLKSCACKLRPYYTKALVVDYVNKKLITCSTLYHGHCDKLDLDNITNKQMFPFMPMVANNATASTVMFIAPGPAVVKDEPQRQVLYVAASYTRTGLKAYREQVPAFCSRSLEDFNLLLKNAFGSSSIEIESQHRDTFPVRYIYGFGSDNFSYVLSIQKANVQTDRYVTKISRICQSDDDYYSYAEVGLSCTMQNTEFNLLQAAYVGKSGTKLARSLGIPTTEDVLYTVFSIGDSNSANPSATSAMCVYSLRDIRKKFTENIQECFSGIGNTGPDHMIQSSKCLKVVSSHFFVIFFLESFESADLSVL* Lottia Protein Model: jg i_Lotgi1_149784_fgenesh2_kg.C_sca_15000101 NR Annotation: PREDICTED: similar to chromosome 19 open reading frame 10 [Canis familiaris] NR E value: 9.00E-20 Sequence: MLISNDFKCLFMLILGFIQW VSSYEMTRDYEEASHFDVMPGGAVLSHEKQLDGFTCKFTYACQGGTKEGWQLTINTNTDKTKYLCSVQRPDGKSYLFFQSFKLEFTGLEVKEGYAMGANFKPLSPSEYQVDFQKHSISDVAGKFGSQLEKVELYGEKGKAEL* Lottia Protein Model: jgi_Lotgi1_150026_fgenesh2_kg.C_sca_22000005

204

NR Annotation: predicted protein [Nematostella vectensis] gb|EDO30774.1| predicted protein [Nematostella vectensis] NR E value: 3.00E-25 Sequence: MLVYLLLAALLTDFSSAGVFYKDCGSVDGTISNIEVSSCASEPCQFQKNTNISVTIDFTSKTVVSSATTVVHGIIAGIPVPFTSVQPDACKDMSCPLASGTKYTYKNQVAVLPIYPTIQLVVQW EVKDQDGKDLLCFKIPVRITN* Lottia Protein Model: jg i_Lotgi1_151060_fgenesh2_kg.C_sca_80000004 NR Annotation: LOC431836 protein [Xenopus laevis] NR E value: 1.00E-140 Sequence: MDFARLFQSLCICLLTLDIQLFGLTSGANPEVEQHLEMGKKLLAAGQLADALSHYHAAVEGDPDNYMTYFKRATVFLALGKSRSALPDLAKVVELRPDFTAARIQRGSVLLKQGKYKEAKNDFETVLKTSPNNEEAKRNIDKIETIPPDIELAYRLVEEGNYGDAIQVLGGIIDTCPWDPTFHEIRSECYEAQGDLFKAASDIRQSTKLRADNTRGYFKLSSLHYEMGEADESLVQIRECLKLDPDNKQCFPHYKKVKKLVKLMTSAQESINNEEYDKCVKKAEEMLKVESLVHHYVSRAGQHKCKCYSKGGKVKEALAECTHLLKIDPNNLDALIDRAEAHIFNEDYEAAINDYQEASNIDGENRRVKEGLNKANKLLKQSKKRDYYKILGVKRNAKKKAIMKAYRKLAVLW HPDKFTTDEEKEKAQKKFLDIAAAKEVLTDPEKRAQFDNGEDPLDPEEQSGHGGNPFQQGFNPFGQGFSGGSFKFHFN* Lottia Protein Model: jg i_Lotgi1_159188_fgenesh2_pg.C_sca_19000225 NR Annotation: predicted protein [ Nematostella vectensis] gb|EDO44886.1| predicted protein [Nematostella vectensis] NR E value: 2e-07 Sequence: MRGPTWSVILLLINLIVTLQACDDVTEFQCADNNQCISKTDTCNTINDCDDGSDENNCACLEKLGMEDGTILDSSLIASSSKTGSSPSYARASNDKGNW CPKSDDSKPFFKVKFDTPRAISAIVFTLKQSVVDPLNYLSTWTLKAVLPGSDEEVTLYEGLNAFSNNSLYRSLFFPSLVTDTFIIAPTTW QNEPCLSFELYGCNIDNLCPTGCQNNGKCSGNDICACADGYFGTKCQFQKTTIPFSSYIFKSLESGSLVTSSETFTVRTSTTIRTSKIIEIQGRKVLSLPVADYFTAPKDGKFGCLSKLGSGGCKSFYLALGCELEELKDTVQLFTTGSDSTYIGAEATLKSDNLVVTFRTSELIWTLNVRKTNMFSSSIKYVLEFDWDISTGIRIYVNKKLIGQQRQATRTTQVLRTNTLATSGVEFFKSKSVNVKLFSLKSSSVNRISLIKTSLISLTPTEECDQEKEHKCANSDKCIDKSNVCDSVEDCDDGSDEQNCPTTTCDVKVYKKLVTKSASLSLDLLEVDDAQRVAKCTLPVISYVVDGILLDFLSKNQYGDQLSATNDLGANQNHNNNNKDDNNNNKKSSQDEFKEDVFNWADEPWTPCDDVEVPDWARMDDPTFDNFNSELIWAEDPWTQLPLVGLPSWMLLPGIDYPVEAKDPVVYTPVNDEDDAAFLASGIDIVNWSDEPYFQLPLDVPRWALLPGIDYPVRHGYNSPYWTYKLNQYNSSRREFGKKMFARQFPTLREERVLYNNNGQSKLCIPGNFQRRNHRF* Lottia Protein Model: jgi_Lotgi1_161657_fgenesh2_pg.C_sca_30000077 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO44809.1| p redicted protein [Nematostella vectensis] NR E Value: 3.00E-17 Sequence: MTKLSEYFLICSMIFVVFMLQRGTCDDVVTEEQISYAKGSVCGYCSYCKFCKLCDKDCPCETSPSKPNCKMCKYCKYCYICSGVCDTICQPGGMIDKISAAIVNALPSFNKEEIEDDLESVKPW LDKKDEL* Lottia Protein Model: jg i_Lotgi1_163300_fgenesh2_pg.C_sca_39000034 NR Annotation: PREDICTED: similar to TTC17 protein, partial [Bos taurus] NR E value: 1.00E-133 Sequence: MASSVVVIVLLHFLFEQCLLTRGSMHW VVTEDGRIQSQADSVYNLRRPYDLVAFMHQEDRATVLNHLKKELLNRKGEIDKSEDRDTTGLEQKFYKSDYDCLQAGTPLPEIDLYISTILPIEHKHISDELSIEIQKAAQSSDNKIGTIPPEPDCTKVLQLDFTPHGFEHLEGVQERMNLTGSAELGLTHAIPFIPDKTEIPDFGHVIAKELKKNSTSWVLYNMAAFYW RVKGDPWMTVECLRRALHFSPRENKDIALISLGNILHRARYSNEAAIVVHSALELSKELN

205

VNHFTLGNIYAVLGEYNKSIICYENTLRIQPDFEAATIRKHAVLCHQKLEAALEAQHRSLQKTLRDLKDYQKKHDLWHIQNDRMSVEQVSQEEKVSQNIAFEFNKAKQTSTKDIGEYCNMVEREGKQVLLCTWNRQAPTLEMLDQFALEEQKKESERNRLKLNKYEKKAIDYNLPVRAPLYVKHDRQAVPRFNELELDNTWPEKEECDSHLLTSPDPFNLSTVYLSPENKGFEVKALLTEAQNLKDGAEHPLPW YPPICVTLLTINEYDEKTYDNLKSVGHPGRTKVPLKMYDPSMRKTLLSHVNGGTVTEEEVGQRILSAIKQEVGARW VLFNLAGLYW RIVGNNYHGIECIRRSLALAPDEYADVPLVNMANILYKW GRYDDAIVLMKDALKINDAEPDSNFLMANLLW VTKNYSGAISHYRTVLDIIPEHQEAFDSLRAIKCLQKFLIKEQSVAPIEVPSQPVGNSCQQKGVANKNQQTESRVICKTENGEEKCIIETRMRGKSGECNGHCTQTCTITPIKVESSCGGPDLAVDTSNPVQCHQKGSQCGEAGEELLFTNDFTNKLDEVSDHYEKKGICNGEDCNTLRVQCLIPMKTHSGLVAHVITPPKLFVRPLSLHSTQCMTEQKKPHIKLEFIDGVLHQKLIFVQDANDIHVEDNECIIFNDGIKSPGCDQAQYRSYSEELARSNINVEFRYVGDESPVEKDKKNHCSKTPNSPGVPSDEIPYSDISDGILQSLNGDVEPSEVVGHRIALTLQQNPKSW VAASAAGLYW RVEGDFTKALDCYRLSLSYAPLEMADLMLLGYANILVQGGYYSNAIMVEDLALDIQSTLPIHYFTLGNIYAALGKWRTAVSFYEATRFYQEDFKPAVDRLRAIHCENIA* Lottia Protein Model: jg i_Lotgi1_165259_fgenesh2_pg.C_sca_50000100 NR Annotation: hypothetical protein CHLREDRAFT_144736 [Chlamydomonas reinhardtii] gb|EDP05543.1| predicted protein [Chlamydomonas reinhardtii] NR E value: 6.00E-23 Sequence: MKVLLVLDICCLIGLVSVGEAVCPDNCNYNGVCDVKHSRCNCYEGFTGSDCKTDCRCNGHGTCQSGSVCKCDEGWKYSGGQCVW DCHCLNGAKCIGPGECGCVHNCKMGNCRNGQCQCW NGYKGSDCSEYDPTM* Lottia Protein Model: jgi_Lotgi1_166436_fgenesh2_pg.C_sca_58000065 NR Annotation: PREDICTED: similar to MEGF6 [Strongylocentrotus purpuratus] ref|XP_001187830.1| PREDICTED: similar to M EGF6 [Strongylocentrotus purpuratus] NR E value: 1.00E-26 Sequence: MMKILEILLCLSFFRATVQQFDCSVCKVCDNSTCIECIEGRYGISCGSFCSLKCSGRCRQSDGYCTSGCDEGYWGPGGLCDQTCSNCNPGGCVMNSGLCIAIGCRTGW YGTTCNNKCNSNCDGNCNQSNGNCNQCIPGKYGTDCQLDCSNCKDTCNKNNGVCNNGCKEGLW GTSCTKTCPNCQSSGCDIDNGNCNNPGCRIGW YGDSCNDKCNERCNDGCKQDTGYCNKCNKGVYGNVCDKNCNKGCLDGCNIDGSCKCKSTYYGTNCSKQCSSNCVDSLCNEISVGYNSCIRGCVAGYKGLYCDKQCPSSCPICNRFDGSCTECSKGFYGTECQLKCPEQCDKCKIKEGTCESCNPKYHGPVCSKRCSDKCLNGECNGVGGRCIDCIPGTFGDNCERSCSNGCIGDTSSTTCDRNGSCISGCINRIYGEKCNLNCSKNCYGSCNRSNGYCNSCLSGYFSDNCNQKCSIHCNGDCDRSTGNCGKCNEGYFGDNCNQNCSINCNGGCQKSSGSCLECNAGYYGDYCKQQCNDKCINNECLRNGQCRFGCMNGKTGSQCDGNCNSNCTICLQTDGNICKECKPGLYGISCDKLCNNTCKKVNSKPACNITTGKCTTGCIDGYKGYYCQDTCLDNCISCNNNQCTSCNTGW YGNQCQTKCNSNCINRCDQATGDCLDGCIDGKSGKHCKQEIG* Lottia Protein Model: jg i_Lotgi1_167022_fgenesh2_pg.C_sca_63000026 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO43826.1| p redicted protein [Nematostella vectensis] NR E value: 2.00E-08 Sequence: MVAVRIRKYKNLLFFALLFAGVLSISTIFYSNHYQIMNTGTRSDVNTGFSYSNANSSKYVIYICDGKNSCGGYGDRQKGIVASYIISVMMNRQFGVIMNDSCDIKQMFKPNQINWIVDNNDIKSKTSKRIKALDGYSDKLRKSMIEMDLDQEYKEEVIYFSLNLEFVFYLRQNKRYEKQLKW LEHLSMSEIYNVVWQSIFKLRPYIREKLDPFLDLKKQGLKLISAQIRLGKNPTIPHDSRVVNSLNNMNVLW QFYKKYNDSSKYRIFISTDSDTVREKARSIFPDVYSDVPGKVFHVERSNKTDICNGW RKVILDQVILSLSDVLVISNSGFGRIAAFFRQNENDLYCLNLDKIRRCTTQSEIFVDKTW* Lottia Protein Model: jgi_Lotgi1_168278_fgenesh2_pg.C_sca_73000118 NR Annotation: no hit Sequence:

206

MSISASSYSGTFKLCLFIVVLVAFSSYCGFVITREDSQFSNHLPKLTSTNVILEKLDSLRKDFLKQRTNVAGQFKNLGSKLSELVLNKNTKPENQHIKAQDEPWRKSTKTGLTLFTSW VDVPEKDLVHGNVVRMW RKWKPLIKPLFFYSSASTGKRLEKEGW LVQPVSKTGCGVTEMPTIKDMFLDAMKNHESVLYGYANGDITFDDGMPKAIDYVVNMDVVREKPVLVLVRRTNVDFSNGPELDANSDITEMYNEGKALKDGSSDGFFTNKLFPWKYLPDIVIGRIGIGMW LASYARAMNVTVIDITKTVKAIHMTTKSGNSESHFKKNGRCNHVIYGRLKLSPTSW GCGFITCATIDITFDKQGQTTMRRKNPKSMNPKCGNCSMDLTQILPKSLGITDRIYFGQNIEPKQVVNKRPKSPK Lottia Protein Model: jg i_Lotgi1_170051_fgenesh2_pg.C_sca_92000069 NR Annotation: hypothetical protein TTHERM_00439050 [Tetrahymena thermophila SB210] gb|EAR97557.1| hypothetical protein TTHERM_00439050 [Tetrahymena thermophila SB210] NR E value: 3.00E-08 Sequence: MKMINIFIVVYCLISPCLGFW LSSKTVDPKVDISTECINKALTGDCGFFTCFEERLPCGEYGYAESYGGKYCWQFQQSHHLFTKKGVEFVEKLTRCHMNRSITSYRQNQIECFSHYDQSFVIMGDCYVESGFCDVVIDNFMSLARILEPKDFTNYRVVREVFRAAKQCPNNISGGIISKFLSYKRGSSL* Lottia Protein Model: jgi_Lotgi1_172278_fgenesh2_pg.C_sca_130000017 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO35011.1| p redicted protein [Nematostella vectensis] NR E value: 2.00E-26 Sequence: MGVKLLFLGICLIYLVLDLHDAEVTAVSDRQAKLKEKDAREKLQQELSKNYAVKQRRRKKKKFRDPRTRGKSSKVVNRGGAPKVQNGDDGGDDPHIGNRENYLQKYFKGKMW GIRDKKKAPKGNW RKKLRKQKHKKKEKRQNMKKKEKSKRTKVKKFKKKRKKHKKRRRGKRSTDENEDDPGSDQEHTHRTKRYAITDQERMW DYGVVPYSFSSDVTDVMARTYRGVFDALEKRTCISFVEW DGTESFYSNRSLNHMVHLNFVDQGGCWAYLGRYTYRTTNIAQDISCCYVGRTCLHEIMHSLSMEHEHTSPLRDGW LRVNW DNLNEDGQFQFSVIDPLMLGRESW GFDIKSFVSYGTTEFTKNGMMGFQPLMPDFYPTGNSYW QPFKEVQMNYDCFGIKCSGMEEPVCENDGYPGYYEGECGCVCPPGLDPATNCASIKPTDPIRGW PPSSFGLPEPIEGCPAGFQLGSLTQPLAGDSEVSSSSHINAGFENSTAEFGFCLKDTASQTEGGLTEWPRGQYCIHSYSNACPPGFQEGLITYDDSVNATRTILTESPLPDGSFVNNTELW YCCRSDGMVQLDIKLPNTESFILYRHGTAECQNVEGMYMFPEYYSLMIEGETIRSISVYQTLGSVPYNVLDGNELFVYYCYYSPLNKDCGEVIHLGPENPTHDIMTPNYPFDYPLNTECNW LFVAPEGSEVMLNFNDFDVEGDDFDCFDYVTIKRGLPGIPGVSYCGTEFKAAIKSIDRYLMITFTSGSEITYTGFHATGRLLDVADLCYNPDDFGRTYNGIHNYAEDFTECLPW VEMAHCDTNLFNTEDAFDNLDGNYCRNPGARYDSPWCYTEKENCTIKYCDVCQERTIYDNFDDCEELIAEHPNFCLDGDHHHYGCLATCIEYGHITIPEKSNRAQDVSCPVPDDIVDADPIVHDVTRYNVGDSLAYVCSNGTATKMRRCLTDGTWTDIEYVCGGCGDEMKSDTRGTCYAYVAEEVRFVEAEEYCIEKDWDVASAQTEDAFNFLVQYRSDMGSDGKQVW LDLYEDPVGVW SW GDESLVQDGFTNWRSTYPVSGPIETDGNNW YRCATMVGTGQSSVWNQVACDNKW YKAHYICQTMESEREICADRKSSCTRNLIDIPLLCSQYQSYAHEVCPYTCGICDVENADSCTVTADFVVGLNLVSGGSTPFSLNPGESFTTQCPAGQICTQNCQHFSRACRRSGEVTGATPVCQDADSVPTLVNNIERIPRLDTSEARRIYQGDNSYMNITRSGEITKWMTLCENDGIVHLMAFKYW SQDRYFKVMGVNQVECKAGRRMTW EIPEGERIQVDPSMNIGLMDWNGGCIPLYECFASEYPDLNKIYHTMAESQDEVALNKLVWMRAGPTCYFFSLNAEISPVGYIAPATTQLTTPSTTTEEPEPEPEATSTSSEDTSESTDMTTSSGDVSTSSTSTSAGNTESSSTGASRSSPSTDAFVSEDTTSSVW QRTTTHSVVITTPRRTINEDFIKSFIKKKKKKRKWWKNRGKKTDKTRKRKNKRRKKAQKGR* Lottia Protein Model: jgi_Lotgi1_176704_fgenesh2_pg.C_sca_2718000001 NR Annotation: PREDICTED: similar to rCG46800 [Nasonia vitripennis] NR E value: 6.00E-09 Sequence: MLTIRVLGVFAVIVLAFVLVESDVELCEDDVNVCHGELPANAMCSGENNKTKRCQCKKGYKMSLDIDCTSNGSVFSCQDIDECATNNGSCEYKCQNEPGSYNCSCPEGSELQGNGLNCGPSGP Lottia Protein Model: jgi_Lotgi1_186451_estExt_Genewise1.C_sca_160091

207

NR Annotation: PREDICTED: similar to out at first [Nasonia vitripennis] (Vital for proper neuronal development and hatching) NR E value: 7.00E-69 Sequence: MSMSLIPSLSYSIFVCVLIKICHTQLVVNVKNGGGDILVESIQSNVSSDTITLEYPSSDGSLITQFIDFKSEAQIFRAYVPGEEERGQPPGSVQVLCFITRFSKNDFISSDAMSKLRQKNPTAIRTPEEERPQEVHSFDLSVDLNKSHVISPHVFNICREAKESTFFNEDDLKTISRSLSKDYNTMMSAMIKLAPTKYGKCSDTSDIFKPCTCRYDACVGWYPCGLKYCRGKDSSGKVVSYRCGIKTCKRCLAFDHFVKQKFLCLWN Lottia Protein Model: jgi_Lotgi1_197084_estExt_Genewise1.C_sca_820083 NR Annotation: glucose-regulated protein 94 [Crassostre NR E value: 0 Sequence: MKRFIVLGILGFLFIAGAGLLNVQAEEVVTEPDVVEDIGKSREASRTDDEAVQREEEAIKLDGLNVAQMKELREKAEKFAFEAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRFVSLTDKSAMAATDELSIKIKADKASHVIQITDTGIGMTKDDLVKNLGTIAKSGTSDFLSKLGESKDQAQMSDLIGQFGVGFYSAFLVADRIVVTSKHNDDDQYVW ESDSSSFSVAKDPRGNTLGRGTMITLHLKEEAHDYLEESTIRSLVKKYSQFINFDIFLWASKTEEVEEPIEEEEEEKKEEEKKEDEDAEVEEEKEEKPKTKKVSKTIWDW ELINNVKPIWTRKPSEITDDEYNDFYKSISSDYEKPMTKIHFTAEGEVTFKSILYVPNSSPSDTFQNYGKQMEQIKMYVRRVFITDDFEDMMPKYLSFIRGVVDSDDLPLNVSRETLQQHKLLKVIKKKLVRKTLDMIKKIDKDTYEKFW KEFSTNIKLGVIEDTTNRTRLAKLLRFSSSNSDTELTSLAEYLERMKEKQEHIYFVAGHNRAEVEKSPFVERLLKKGYEVLYLTEPVDEYCIQSLPEFESKKFQNVAKEGLKLDDSEKAKERKEATEKEFEPLITWLKDSALNDKIEKATISERLASSPCALVASQYGWSGNMERIMQAQAYAKAKDPSQEFYATQKKTLEINPRHPLIKELKKRVEANKEDETAKDLAVVMFETATVRSGYRITDTAGFAQRVENMLRLSLEVPLDAKIEEEPEEEPEEAEEEKTEEVEAEAEEEAEEKKSEHEEL* Lottia Protein Model: jgi_Lotgi1_203670_estExt_fgenesh2_kg.C_sca_170029 NR Annotation: PREDICTED: hypothetical protein [Ornithorhynchus anatinus] NR E value: 1.00E-142 Sequence: MKLSVFLSLLFLTAVWADTPANCTFDDIKGTWTFFIGSNGHDNTVNCSNMGNPKSQLQVTLYFPDVAVDEFGNKGFWTIIYNQGFEVVISGRKYFAFSMYEQSGSTVSSICDKTLPGWSHDTFNRDWACYYGKKITSVPPKYYKKNKPHRLFKLNYDYINNINKQQKLWTATVYPEYEGKPMRELLNKAGGPASQVFGPIKAAPVTW EQQRLSETIPEQFDW RNVNDNNYVSPIRNQESCGSCYAFSSMAMNEARVRIMTNNTLQPVFSPQDIVECSEYSQGCAGGFPYLIGGKYAEDFGLVEEKCNPYKGQDHSCSTDKTCKRQYATSYRYVGGFYGGCNDALMKMEIYRHGPVVVGFNVTDDFFHYKSGIFIHTGLTDRYNPFEVTNHAVLVVGYGTEKGVKYWIVKNSW GEKW GESGYFRIRRGTDELGIESMAVSSTPIF* Lottia Protein Model: jg i_Lotgi1_204770_estExt_fgenesh2_kg.C_sca_820009 NR Annotation: unnamed protein p roduct [Tetraodon nigroviridis] NR E value: 3.00E-35 Sequence: MAATTHKMFLVACLVFLPIFASADTCLGPSVSAETYTTSEVTVSTETVFIAQFTLTCKNGLQNVNLYAEVAGRMIPAVKTSKPNEYQISITDEYKALRSGTYEAKFYDEESFSALKKAKRNNEDSNSVTPLFSINISHSGLWQGSYIQSEFVAACVAILVWW LAFSAKSKLSP* Lottia Protein Model: jgi_Lotgi1_205030_estExt_fgenesh2_kg.C_sca_1120002 NR Annotation: PREDICTED: hypothetical protein [Strongylocentrotus purpuratus] ref|XP_001188672.1| PREDICTED: hypothetical protein [Strongylocentrotus purpuratus] NR E value: 3.00E-20 Sequence: MKLMFLGFIGILMLSTSNNASASPWFCKGLECPKFNLLNKTKDYEVRQYGSLTW VTTSHIGQWKSDILGEL

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LFKNLFSYIEGNNTMKKQLDLAVPLLTKLESAGDRGSIYTMHLLIPRENIDHLPSPLNTNLTFITIPPSTYYIRSFDGVAYDRQYMKVLQELVNAINDTGAYPDDFFYTATYDHPQVKENRHNEVW LLKKEKIP* Lottia Protein Model: jg i_Lotgi1_211937_estExt_Genewise1Plus.C_sca_110124 NR Annotation: PREDICTED: similar to SPFH domain family, member 1 [Monodelphis domestica] NR E value: 1.00E-124 Sequence: MANPALALLSVSAVALAIFLNFSIHKIEEGHVGVYYRGGALLSSTSEPGYHFMLPFLTTFRSVQTTLQTDEVKNVPCGTSGGVIIYFERIEVVNKLDRAIVYETVRNYTADYDKTLIYNKIHHELNQFCSVHNLQEVYIDLFDQIDENLKKALQLDLNVMAPGLMVQAVRVTKPKIPESIRKNYEAMEGEKTKLLIAIQKQKLVEKEAETERKKAVIEAEKVALVAKIQW EQKVMEKESEKRMSEIEDSANTARAKAKADAEFYKTQKEIESNKLKLTKEFLEFTKYESISKNTKIYFGNSIPGIFSDKVEPQEIAKVVGERK*

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Object 2-12. Predicted secreted signaling molecules found in Nautilus pompilius.


Lottia Protein Model: jg i_Lotgi1_110650_e_gw1.12.27.1 NR Annotation: unnamed protein product [Tetraodon nigrovirid is] NR E value: 1.00E-32 Sequence: MKIILVLLCFCHLIYSAKTCPSNCQCHLTDKAVNCYKRELQFVPANIPLDTEILDLSSNDIANIDPTMFHNLTKLTDLVLNDNKISKLDPRTFQNLHKLEVIKLSQNFLSTIPEGLFDNLPIMVPKLMELNLSQNKIVKIENYVFRPLSMLSTVSLNDNPLKNADRLLSKNRRLSYIDMSECQLTTVPRGLPDSIEHLKLIKNNITKIKPKDFRNKNYLKILALDENEIEFIAPNSFKSLQKLIQLW LNGNRLRKFPSPISPSVTNVYLAKNNITKLSAADF Lottia Protein Model: jg i_Lotgi1_119019_e_gw1.30.87.1 NR Annotation: PREDICTED: similar to seleno protein N, 1 isoform 2 precursor [Canis familiaris] NR E value: 2.00E-85 Sequence: MVLFTLLCCLFIIGRHDPTQIPEEIVINIGDDGVLLFQQHDRDNDGYLSIQEFEPLVYRLLEINVSGPVYDVPISTDDEMITLKSYFIPIVKESMSKDLNDSVSIGLLRTMNSLHGLEKW QNVNLQWMNFGASHFSGFLPKDVDSIMLGSSYFIINVEKGLFNAALSSNRYYPPKVTTNESIIVHRLLTLFHPRPFVVSRFPAQSSVACVRAYNDKYLDIVFRIHAEFQLNEPPYHPFWFTPAQFTGNLIISKDGKHIQYFNLYVPNNKRLNIDMEWLNGPNESENMEVDIGFMPLMQLNSTQSSVPVKNLEEYELLEPLPERPQHKTEGENIEWMATIDLEDAKSSLEKALYPFKKVPYYNFTEAFKKAEDNKKLVHSILLW GALDDQSC* Lottia Protein Model: jg i_Lotgi1_121665_e_gw1.37.7.1 NR Annotation: hedgehog [Patella vulgata] NR E value: 1.00E-171 Sequence: MKLFFSFQSVCSWTFIFLFITSLTHACGPGRGSGSRRRPRKRTPLVFKQHVPNVSENTLGASGISDGKIRRNSEKFKNLVKNENPDIVFKNEEGDGSDYLMSRRCQDKLNSLAVSVMNNWKDVRLRVTEAWDDSPNSHAKDSLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDW VYYESRGHIHCSVKSDSSVAIKIGGCFPPTGSVQTLHGWKTMGQLTVGDKVLSINSAGQLEYSPVIAFIDRNDLEFEKYLTLHTEDDTDITLTSKHLIYASGTNSSNFESYDVVYADDIMEGDHVLITSSEKGAISPTRVVTISEKTLQGVYAPLTVNGNIVVDGVVVSCYAVVSDANLAHAVFAPMRGLHYLSQYVPW FLHSTQQENAPQNGVHW YAKMLYNIGSTFLNEKTLYIA* Lottia Protein Model: jg i_Lotgi1_137329_e_gw1.113.40.1 NR Annotation: insulin-like growth factor b inding protein, acid labile subunit [Sus scrofa] gb|ABE73450.1| acid-labile subunit [Sus scrofa] gb|ABE73451.1| acid-lab ile subunit [Sus scrofa] NR E value: 1.00E-21 Sequence: MSFVQLLPFFLFLTISQSSSQRWPLSGTCYPCSCDINQKGFGRVKYVNCSNLALTRIPRNLPLDLHTLDFSNNRILPQEIQQLCTFNSMQYVALSYNSLDSIPPEIFKDLENLHTLVLHGISSIPTTNIFEDLFNLEYLDINDCHVNQIPDNWFRKLYSLKTLKLRQTGIRSIEPGVLDGLLNLQELDLSHNVMRTAQTESFKPIVQSIRRINFRGNMFKTIGDHLFEKMYNLVELDLSDNKLESIHKRSFLDLRQLINLDIRDNKLSNLPGGLFKNLRNLQTLNLAMNTFNNFPETLFKIGYLLKLDLSYNRIRRLPDSFVHSFPYLEFLNVDKNPLHCDCKSLDVKFYQPNLVII Lottia Protein Model: jg i_Lotgi1_152641_fgenesh2_pg.C_sca_2000061 NR Annotation: interleukin 17 [Crassostrea gigas] NR E value: 5.00E-07 Sequence:

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MVLMKVFICALLVNMVYSFTLIKNIEHILERERRSDECAVPNNIDELFTNLNSHVNRVEFLIAVNATHQPNAQGLIPPAAGDQTDTECPKEYTGKDTSNSGIW QRSTCPWFYSERNYGAEYYPSKVYNAACKCVDCLYSKSNSETGCSRIYRNINILKKTGCSNGFYTYEQRSISVSVGCTCAKLQANIG* Lottia Protein Model: jg i_Lotgi1_163440_fgenesh2_pg.C_sca_39000174 NR Annotation: No match found NR E value: Sequence: MDLYGVVW LLAILQEVTSCFLTEKLPSLEGKEWFTKIRSGGSNDVIAYFDRNSVTFNDVKNTTNGETKW RCFMKIRGRYFLKNVRQGGGYLYKCMGIIVRSESAIQLEWSHVSRLADPALCAEENINLDPW LLISYKTVVNDFTSCPVSGGFDIKHAGYYSRDVRDSRGYDIGCNLMNIPMRLEFDCIAGEGAIFNFRSKNCVPDIHFNIYQNTICVAKWSNKRHHFVLLRTKTGLEFW CARFPVGIENQNETELYLYSDVACLEGEYAEEFVKFMKFDLQRVMYTTICADEYPQCKEGTCNVFSKLECQKTCGLCNPHRPPGICTFPKRMQGTYLLHSKHGNQNVTLEGRTLNIENVGQFDCIVFEDSPLRSTKTYTTMSIFQNGCRPRYTCVRLKRLGPSALRYSLAQNLVW PIKKERIGANICNEDNFRADGDPIRDTFRSYKKTGKPVIKIHPKPRFINCGMNTSYTIRAELPDGSICHGGFYQHCKNETKLRFDFHTCNSFIKPKEDFNCAGIFRTNYW ERAVLIQNVDNLNDIRCLIFNSLRPYQALIVVADECDKMASGLVDSNIRIPIMRLHIRSDVYPCKHIQLPLKDDVITNVVTTTEPIKMNVTLKESKNVNSFSVDLELSSRNSSGSRPFENKLNSLLLLFLCYVVFVKNL* Lottia Protein Model: jg i_Lotgi1_164016_fgenesh2_pg.C_sca_43000014 NR Annotation: chordin [Danio rerio] sp|O57472|CHRD_BRARE Chordin precursor (Protein chordino) gb|AAB93485.1| chordin [Danio rerio] NR E value: 1.00E-119 Sequence: MSPMDLAVCLVLLAATVSSTKFSKIPLKAARENLFGIQPNRPGCILGSKFYGI GERWTPELLPVGEMYCVKCECVPVERKGIIDMKGQTLCKNIKNLCPRPNCLNPVLPKGKCCKVCPDEVNSFEDSFSLGSKSSAPTMISSDSKRRVRNEFVSLLVGKNVRRESVKTSAVAAVYFSIVGRDEIRYSVRYFKLDRPKFLQITDANGNVLFERPIEKKRNGDKKFCGIWTKVPSAYIQYVREERLFAVITASRHFRGLVSGRIMVNRHAKREVFSSVLASPKANGIGGLLSVVYNPRTHLVDYVINLDGVFPGSSEEYFITISKKSKVLHQSKGKASSKTRFIRGSWTLKNKRQSKQLARGRLTIRLTSTNGASVSGDIKPKVTCGVSQAVMSSGNSLEEMKLASSGTAVFELREKGSLDYKIRVTGLTSKVHRIRLEGAVNKKNRRQIIGSVHRNFKADENSFNGW CNGTFKKMTADDLHLFLNNKIFVNVATSENRISELRGRVIALPYHSHFEQVSADPAALGPKTITSNGHAGNAWISMGVGCSLHYDITTTTIAKHHDLTLSLVSPTDIDAYVTIEKISTTNLYEFQQASGSIAVVPESLFRDLDEGSALIQVISGDVILSGNVSVPNTCWQYSQDYDMDILVDEQTSEEVLAANRYKCVYEGKVYENGDSW LPDVNATCNTCSCNKGKIECHRLICPVVECANPIVLDGECCPTCPIETTDQTECKIDGDLRSHPVGSTWHPFLPLMGYAKCVICTCLPGGIYKCERKCPKLDCPASRRIRLNPKDCCQVCAPEEVKPEVVKPLQDVDMKGACSFKDQIYANGAKWHPRVIPFGYMKCIDCSCNNGTPSCSRPKCPKLSCSRKIREDGACCDTCADSDGEEIVEEKKSDSCIFGGKKYDDGETFQPKTSSLSSCATCTCRKGSMKCSLTCPEKCKDPKHSNDPCCKHC* Lottia Protein Model: jg i_Lotgi1_167943_fgenesh2_pg.C_sca_71000005 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO34356.1| p redicted protein [Nematostella vectensis] (Fulicin-like) NR E value: 1.00E-21 Sequence: MKSILVTSVVVVAGTLLTLVQALGADKFAEPATQKHSKIPTAALSHSGDTLLNTDAHQTNPSNIWKSKSKFDLDALPVVNRQDVQSHYAQHRRSYDTPMFLGKRDLKNLSLRFKRATDLEDLDATEFSDDFDKRGVDIPQFIGRRRYDTPQFIGRRGYGVPAFVGKRAFRAPAFIGKKAFGAPAFIGKKAFDAPSFVGKRKLTAPSFIGKRGLDVPAFVGRRAYAPPQFIGRRGMDVPAFVGKREFQTPMFLGKRAFSAPSFVGKRGLDAPTFIGKRKEESFRDLLATLQAYREYRRLMQTDKRFDAPLFVGKRSDYENDNLAAETLSRRFQCIPQW VFRMRLNVVDLNMSFIGGYKQNYFSLNILDDVQTKILLPLDDLHKVDSSKMDVPTVQLWDADMPRPNGARFLKLDPAHALGFLGQEASPVSKRYLEFVGKRDNDKRYAEFVGKRAGFGEKRYSRGSLSEYLSKRLRIRRPEFVGKRSNQKRYLEFIGR*

211

Lottia Protein Model: jgi_Lotgi1_168278_fgenesh2_pg.C_sca_73000118 NR Annotation: no hit Sequence: MSISASSYSGTFKLCLFIVVLVAFSSYCGFVITREDSQFSNHLPKLTSTNVILEKLDSLRKDFLKQRTNVAGQFKNLGSKLSELVLNKNTKPENQHIKAQDEPWRKSTKTGLTLFTSW VDVPEKDLVHGNVVRMW RKWKPLIKPLFFYSSASTGKRLEKEGW LVQPVSKTGCGVTEMPTIKDMFLDAMKNHESVLYGYANGDITFDDGMPKAIDYVVNMDVVREKPVLVLVRRTNVDFSNGPELDANSDITEMYNEGKALKDGSSDGFFTNKLFPWKYLPDIVIGRIGIGMW LASYARAMNVTVIDITKTVKAIHMTTKSGNSESHFKKNGRCNHVIYGRLKLSPTSW GCGFITCATIDITFDKQGQTTMRRKNPKSMNPKCGNCSMDLTQILPKSLGITDRIYFGQNIEPKQVVNKRPKSPK Lottia Protein Model: jgi_Lotgi1_169325_fgenesh2_pg.C_sca_84000054 NR Annotation: Insulin precursor [Contains: Insulin B chain; Insulin B chain'; Insulin A chain] gb|AAF80383.1|AF160192_1 insulin precursor [Aplysia californica] NR E value: 4.00E-13 Sequence: MEVTCKCPLVLLGVLFLNFGTVLTHLEWTCTLETKRESPRGVCGQRLPEVLSMVCKRYGGYRDTWFRKRNGEGTNSRLGNIILGKRDAFSYLGKRGQSYGEQGITCECCYHSCSFRELRQYCRNSQQRISIKK* Lottia Protein Model: jgi_Lotgi1_179503_fgenesh2_pm.C_sca_71000002 NR Annotation: Wnt7 [Euprymna scolopes] NR E value: 1.00E-122 Sequence: MTVSPTVVWAAFSMALSSVVALGANIICNKIPGMAPKQRAICRSRPDAIVSIGEGAKLGLTECQYQFRFMRWNCSTLDSNDSMFGYESLGGTKEAAFIYAMTSAGVSYAITQSCGLGSLPNCGCDKDKGDGKLAPQGWKWGGCSADIKHGLRLARKFMDAREIAQNARSLMNLHNNRAGRKAVKDNMGTDCKCHGVSGSCTMKTCWTTLPPFRKIGDSLKKRYKKSKIVVPYLGRRARTAVTLILKRAKRPHRKPRRSHLVYLDKSPNYCDFDGKTGSLGTVGRKCNRTTKDTDGCDLMCCGRGYNTHQYTRTWQCNCKFHW CCYVNCNKCSERTEEYTCK* Lottia Protein Model: jgi_Lotgi1_188920_estExt_Genewise1.C_sca_260301 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO37057.1| p redicted protein [Nematostella vectensis] (growth factor) NR E value: 1.00E-109 Sequence: MFLEVFNISATVLCILFLIGHRKCDTIQNSKHHAELMMDLLQPYKNYHGQRLKKSNRHIRECQEIKYGNVTHSKVKVKPDTNISYPLVEVKHLTKEIGSYYYKNPVTIRYQRVDFPLNTLSVLEPGEPGTCREGHGTRSITSKTALSGNCIVGVNGGFFNTTTGACLGNIVSNGRLVQDSQGLQNAHFGITEDGYIYVGYLSELDLITQQFKQLLGGVLW LIRDGEKYIDKSKQMECPDVEETGTIETFIEVQSARSAVGHDKDGRVIMVQVDGKTNARGVNLHEFTDILLELGVVNAINLDGGGSTTTVINHTLVNYPSDKCADGTFSCEREISTILCVHEPECQPNDCNQHGHCVLGKCVCDGYW VGSDCSSLSCPFSCSHHGTCTKDGCICDDGW YGINCSTPCKPGYYGSNCAHQCYCLNHGTCNSQTGHCVCSPGFTGKYCESLCEFGYYGDKCNNLCTCDNGCFCHHVTGSCNLS Lottia Protein Model: jgi_Lotgi1_208417_estExt_Genewise1Plus.C_sca_10710 NR Annotation: fasciclin- like protein NR E value: 5e-178 Sequence: MSRNLWIFLLILLSIIVIASSRRSRGRW YRGRSGRRSLADRLRDRGTKPSNHRPRQRHGW GRW GSGW GAGQDHRTRVEEELDDLFRRIKDRRYGFNFEDKFW LDFGWNSRNEPKPWWEGPNVCKDEKESIENTSISDTGPISRHFSFKNQVCDQSETSYKCTTKTGIFMKQKTVTEVYECCPGFTRKDEMFGCPTEFKLDGLVKTARNLGLTEFLRATESVSLTDDLEKGNFTVFAPVNKAFEDEKNILPELETILPKDMPSVVMVSKPLTDLVIADMSNVLLGHLVYDTLKTSGLNDEQLIETASPFDSLLRVNFFSRPEKLITVNCARILSADNQATNGIIHTVDRIIKPVTDSIVDIISQNPNLSYLKTALARADLVKALRGTGQYTLFAPTDAAFEKLEPSIRDRLIKGEKSCLEKVLKNHLLTSVIC

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STVIQGTVTTTNLLNKYLNLTRTEDDKLFVEGSQMVGRDIMATNGVIHVIEDVLVPDQALGLIDVAEKNNLTKFVELLKETGMFKGITKMENLTLFIPSNNAFEKLEEKVSLEELKKDKKKVEDIVMYHVVPEVVTCSRLHNNYKLSTENERNIRMNRYSTFPFRHHSIQTAQCAPIIKENVPACNGVINIVNEVLLPPVGNVVDNLASKKKFSTLVRLMKKSGLADMLQEEGPFTMFAPTNEAFDDLYEEDLEKIEDNPEVLKKVLQNHIVQEFVCCASISMVRSWFDVASVRTLSGVRFPVKERHGTIQFNHAVVTECDNTATNGVVHAIDKVTLSPERPFWKRDFYHW* Lottia Protein Model: jgi_Lotgi1_168619_fgenesh2_pg.C_sca_77000042 NR Annotation: crossveinless [Saccoglossus kowalevskii] (a new family of twisted-gastrulation-like modulators of bmp signaling) NR E value: 1.00E-148 Sequence: MYYTELSILAKCIALCILTCFPILIQAQLTGTVTRCYNEGGEVKIQGITTDPCISCYCMDGYVQCDKKKCDNLDGCHAILFDGPKRCCDTCKGCRLNGVKHESGKSWFDKRDRCTSYSCKAGVITKSKVQCHVPCSNPIKRKRQCCPSCKGCHFDGADRKNGEKFALASDPCVECKCRKGSVTCMKRACPVLNCPDEVIYQPKGECCPKCKGMRKIFDFPGACYFAKRVYVDGFSFEPKSETRCTCTKGTVICEKATCPPVACPVEERVQKGSCQVCEPKRNCLYDGKIHMHRSKWQPRMCTQCSCQNGVTYCQRERCNNSLSCPNGYKLQFQPGECCPKCVEHDAVCRVFGDPHYRTFDGKMYNFQGTCKYMLSQDCQGKDFTIKVKNGVRLSSGFAWTQMVVVLMGDTRISFRQNLLIKINRRRVQLPYTIPGKFSIRREGHSVTFRADIGLKVVW DGDSFLEVTASRKYKNRLCGLCGNYNGLETDDLIGRKGKNYLRGEEFGNSW RIGSKKACKTQPKVKNLQSICDKDFKAKVRANKECSVLYSRAFSSCRRVVDVTPYVTSCVTDMCDCPHGKKCSCESIQAYAHECKRAGHKVKW EKVSNCKAPQKNCPKGAYYSLCAPACPKTCSSNKPDGSCSKKCSPGCVCQNGAVLYKNRCISPEKCPKT* Lottia Protein Model: jgi_Lotgi1_205842_estExt_fgenesh2_pm.C_sca_70027 NR Annotation: BMP2/4 [Patella vulgata] NR E value: 1.00E-159 Sequence: MIADFYRSVVLLLLVLVVNYTSSLSTNTLHPRKHGNEKVLAAVESNLLGLFGLKSRPVPGRRTPIPDYMLDLYKRLDSEPDFISPFAESKGKGIVEANTVRSFYHSDINFVKDCDERTCARIWFNISTIPFAEIMAASELRVFKDVYNHFKLTDANSLSGDRIKASLFKHRLEIHEIMRVSPDDSECISRLIDTKIVDTRNSSWESFDVHPAVLKWRRRPQYNYGLEVRIVSKSPWISTNSHVRLRRSAHMDEKNWQIQRPILVTYTHDGRNPNSRIKRASARNRRRKSRKRRRRKKKGNKNECKRHALYVDFGDVGW NDWIVAPTGYNAYFCRGECPFPMGQHLNSTHHAVMQTLVHSVNPSAVPKACCVPSELSAISMLYLDEW DKVVLKNYQDMVVEGCGCR* Predicted molecules found in Nautilus pompilius with unknown function or predicted to be


Lottia Protein Model: jgi_Lotgi1_103075_e_gw1.1.1164.1 NR Annotation: PREDICTED: similar to CG31650-PC, isoform C [Apis mellifera] NR E value: 9.00E-50 Sequence: MNVYYNILILCLGVYCTLGEKEKVPNEPVDQKHTKDGEHNPHYDHEAVIGSKQLEHEFDELPPAEAIQRLENLVKHHDLNADNIITHDELKKW ILASYMSLDEEESDEKFQENDVDGNGNVSW RELVKKEFGYDLEDIEDFKKNTDKQEPDTKEFLLMLDEEEKRFMTADTDNNGNLEKQEFRAYYHPNEYPHMHGVEIERALRTHDKNKDNQVSKAEFIGGLLFLEDREVYLTEEENFNSFDLDGNGKLSQDEVRAW VIQDNNQAAEDEVLHLISQADDNKNNELTLAEILEHYDDFVGSATDYGHKLSDEL* Lottia Protein Model: jg i_Lotgi1_132441_e_gw1.79.137.1 NR Annotation: PREDICTED: similar to CG11081-PA [Nasonia vitripennis] NR E value: 4.00E-72 Sequence:

213

MAWKIYVLFVCVLCLRFILAARENYIKATFEGAKDRGIFNKLVVNRETNELYLAAVNHLYKLSPNLEVIQEVVTGPHLDDPSCTSLTNLTCDPDLKSCACKLRPYYTKALVVDYVNKKLITCSTLYHGHCDKLDLDNITNKQMFPFMPMVANNATASTVMFIAPGPAVVKDEPQRQVLYVAASYTRTGLKAYREQVPAFCSRSLEDFNLLLKNAFGSSSIEIESQHRDTFPVRYIYGFGSDNFSYVLSIQKANVQTDRYVTKISRICQSDDDYYSYAEVGLSCTMQNTEFNLLQAAYVGKSGTKLARSLGIPTTEDVLYTVFSIGDSNSANPSATSAMCVYSLRDIRKKFTENIQECFSGIGNTGPDHMIQSSKCLKVVSSHFFVIFFLESFESADLSVL* Lottia Protein Model: jgi_Lotgi1_150026_fgenesh2_kg.C_sca_22000005 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO30774.1| predicted protein [Nematostella vectensis] NR E value: 3.00E-25 Sequence: MLVYLLLAALLTDFSSAGVFYKDCGSVDGTISNIEVSSCASEPCQFQKNTNISVTIDFTSKTVVSSATTVVHGIIAGIPVPFTSVQPDACKDMSCPLASGTKYTYKNQVAVLPIYPTIQLVVQW EVKDQDGKDLLCFKIPVRITN* Lottia Protein Model: jg i_Lotgi1_151060_fgenesh2_kg.C_sca_80000004 NR Annotation: LOC431836 protein [Xenopus laevis] NR E value: 1.00E-140 Sequence: MDFARLFQSLCICLLTLDIQLFGLTSGANPEVEQHLEMGKKLLAAGQLADALSHYHAAVEGDPDNYMTYFKRATVFLALGKSRSALPDLAKVVELRPDFTAARIQRGSVLLKQGKYKEAKNDFETVLKTSPNNEEAKRNIDKIETIPPDIELAYRLVEEGNYGDAIQVLGGIIDTCPWDPTFHEIRSECYEAQGDLFKAASDIRQSTKLRADNTRGYFKLSSLHYEMGEADESLVQIRECLKLDPDNKQCFPHYKKVKKLVKLMTSAQESINNEEYDKCVKKAEEMLKVESLVHHYVSRAGQHKCKCYSKGGKVKEALAECTHLLKIDPNNLDALIDRAEAHIFNEDYEAAINDYQEASNIDGENRRVKEGLNKANKLLKQSKKRDYYKILGVKRNAKKKAIMKAYRKLAVLW HPDKFTTDEEKEKAQKKFLDIAAAKEVLTDPEKRAQFDNGEDPLDPEEQSGHGGNPFQQGFNPFGQGFSGGSFKFHFN* Lottia Protein Model: jgi_Lotgi1_118302_e_gw1.28.365.1 NR Annotation: No match found NR E value: Sequence: CVMLYMLCKCSSLDCVKLYVLCECSICHCVKLYVLCECSICHCVKLYVLCECSICHCVKLYVLCECSICHCVKLYCSICHCVKLYVICECSICHCVKLYMFCECNICHCVKLYVLCEV* Lottia Protein Model: jgi_Lotgi1_154212_fgenesh2_pg.C_sca_5000252 NR Annotation: pred icted protein [Monosiga brevicollis MX1] NR E Value: 2.00E-97 Sequence: MNLKLYILICVTVSGILGTPLDDYVNRPDSTYKYFEITHYVHEDHTLYILNMTSQTW LTAEISTQPIWWHYLTVTIPHKIRYPDAAFMFIEGGSNHNGPPTGTDNFVALTTAMAVSTGTIGADLKMIPNQPIMFKADPTKKQRTEDAIIAWTWKTFVETNGSNPDLLLRMPMTKASVRALDTIADFAMDKVGNKVDKFFIAGASKRGWTTWTTAAVDNRVVAMAPIVMDLLHMVKNLHHHYRAYGGWTFAFKDYYSLNFTQDLDSPWTQAMADIIDPISYNDRYANIPKLVVSTGGDEFFLPDDSHFYFKELMGPKYLRILPNAEHSCAGHEISLLFTMRAFYLSVITKTPLPQLTW VREETPSGGRITVTCNQKPKTVNVFYARTLDGNRRDFRLLVGTPGDPSKPMPHPVVW LRDDVQDMGNNVYRAELTKPEIGW TAFFIQLTFQGFADSDLEFTTETQIIPDIFPFPDCTGQECKGTLV* Lottia Protein Model: jg i_Lotgi1_159188_fgenesh2_pg.C_sca_19000225 NR Annotation: predicted protein [ Nematostella vectensis] gb|EDO44886.1| predicted protein [Nematostella vectensis] NR E value: 2e-07 Sequence: MRGPTWSVILLLINLIVTLQACDDVTEFQCADNNQCISKTDTCNTINDCDDGSDENNCACLEKLGMEDGTI

214

LDSSLIASSSKTGSSPSYARASNDKGNW CPKSDDSKPFFKVKFDTPRAISAIVFTLKQSVVDPLNYLSTWTLKAVLPGSDEEVTLYEGLNAFSNNSLYRSLFFPSLVTDTFIIAPTTW QNEPCLSFELYGCNIDNLCPTGCQNNGKCSGNDICACADGYFGTKCQFQKTTIPFSSYIFKSLESGSLVTSSETFTVRTSTTIRTSKIIEIQGRKVLSLPVADYFTAPKDGKFGCLSKLGSGGCKSFYLALGCELEELKDTVQLFTTGSDSTYIGAEATLKSDNLVVTFRTSELIWTLNVRKTNMFSSSIKYVLEFDWDISTGIRIYVNKKLIGQQRQATRTTQVLRTNTLATSGVEFFKSKSVNVKLFSLKSSSVNRISLIKTSLISLTPTEECDQEKEHKCANSDKCIDKSNVCDSVEDCDDGSDEQNCPTTTCDVKVYKKLVTKSASLSLDLLEVDDAQRVAKCTLPVISYVVDGILLDFLSKNQYGDQLSATNDLGANQNHNNNNKDDNNNNKKSSQDEFKEDVFNWADEPWTPCDDVEVPDWARMDDPTFDNFNSELIWAEDPWTQLPLVGLPSWMLLPGIDYPVEAKDPVVYTPVNDEDDAAFLASGIDIVNWSDEPYFQLPLDVPRWALLPGIDYPVRHGYNSPYWTYKLNQYNSSRREFGKKMFARQFPTLREERVLYNNNGQSKLCIPGNFQRRNHRF* Lottia Protein Model: jgi_Lotgi1_160458_fgenesh2_pg.C_sca_25000063 NR Annotation: predicted protein [Monosiga brevicollis MX1] NR E Value: 6.00E-86 Ac Annotation: no hit Sequence: MNYKMILNKYTLVLGIILVCTQPCITDVNITKVHLIFMNHLDVGFNGITPQTGFVINVINKYFTKYFPGAIELALQMNFLGYRERLIYTTHPW LVSLYLDCPQNLTMSGIQLMCPTEEQREGFLYAAKRGDITW HAGPMNMQYEALDTAMVNFSLKLSEDMDARMDIKRRHRVLSQRDVPGMTRALIPIFNNHGIEGVTVGVNSVSSPPAVPKIFKW VFQNSSVIAMWHPGGYPIKPGSFPSMPLGLSRDNCVTFEQFDEAMCFSFRGDNQGPPTSIAEVLNSYEIVRGQFPGAIVEGSAFENFVEVVQSVKDKLPVVDQEIGDTWIQGISSDPGKTAMMRGFFRARTACLTSAPNFEDVISSWSEQRQFIDLALETLGDHPLVQAVEEEYKQLVPQAPDLSKYKQLSATTFDCQNGFKFSFAADGSLNKLQDKDGRNWASKSQPIGQLLYKTYNDTDFDSFQKQYVLNHYYW FQVGKWNLTANCPTCESTIWNTKLQKLYAASDGSCDVVALLAMEEDRATSFYGAPPKIYVKYLSSSNTFLSIEVQLFGKKPTRLAESLSLSFQPIRQEGSKW YLHKLGQLIDPLNVVTNGSQRLHAVDEGVQYKDSNGKGMMIGSKDVGLATVHQTPTDVSVLPVPLTPIQSISGVSYNIFNNVWDCNYIFW YPFLKQDSNSKYRFSITFS* Lottia Protein Model: jg i_Lotgi1_160696_fgenesh2_pg.C_sca_25000301 NR Annotation: No match found NR E value: Sequence: MKLLLLLTVLIPLCVSVPTKPGCCVPPVW EGIQGMFSGDGSGPAPELIQQSALISYDEKRQMININITQSIHGLTSDVRILTDYKKGVQYLINSKSRSCTTSKVPQTFQRFCIPDYFYYKGSFNVAFTLRASTEQFIVPLIWSKCKSNSSNFFKMRKACFSRDIQLVVGTCDCLFN* Lottia Protein Model: jgi_Lotgi1_161657_fgenesh2_pg.C_sca_30000077 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO44809.1| p redicted protein [Nematostella vectensis] NR E Value: 3.00E-17 Sequence: MTKLSEYFLICSMIFVVFMLQRGTCDDVVTEEQISYAKGSVCGYCSYCKFCKLCDKDCPCETSPSKPNCKMCKYCKYCYICSGVCDTICQPGGMIDKISAAIVNALPSFNKEEIEDDLESVKPW LDKKDEL* Lottia Protein Model: jg i_Lotgi1_161950_fgenesh2_pg.C_sca_31000192 NR Annotation: RE49668p, putative [Brugia malayi] NR E value: 3.00E-08 Sequence: MKLGGDYGILCVLFTILTPVISIDCYACTSLGGNNEGCEDSFERTLKTVHFIARDCYYGYFRGTHCYKLKGERADGSSIMVRHCSDSHW GSHCGDIKYTNEHNVEEKIKGCLETCDHDGCNTATQKQFNFSLVIMAVLMTSALLL* Lottia Protein Model: jg i_Lotgi1_163300_fgenesh2_pg.C_sca_39000034 NR Annotation: PREDICTED: similar to TTC17 protein, partial [Bos taurus]

215

NR E value : 1.00E-133 Sequence: MASSVVVIVLLHFLFEQCLLTRGSMHW VVTEDGRIQSQADSVYNLRRPYDLVAFMHQEDRATVLNHLKKELLNRKGEIDKSEDRDTTGLEQKFYKSDYDCLQAGTPLPEIDLYISTILPIEHKHISDELSIEIQKAAQSSDNKIGTIPPEPDCTKVLQLDFTPHGFEHLEGVQERMNLTGSAELGLTHAIPFIPDKTEIPDFGHVIAKELKKNSTSWVLYNMAAFYW RVKGDPWMTVECLRRALHFSPRENKDIALISLGNILHRARYSNEAAIVVHSALELSKELNVNHFTLGNIYAVLGEYNKSIICYENTLRIQPDFEAATIRKHAVLCHQKLEAALEAQHRSLQKTLRDLKDYQKKHDLWHIQNDRMSVEQVSQEEKVSQNIAFEFNKAKQTSTKDIGEYCNMVEREGKQVLLCTWNRQAPTLEMLDQFALEEQKKESERNRLKLNKYEKKAIDYNLPVRAPLYVKHDRQAVPRFNELELDNTWPEKEECDSHLLTSPDPFNLSTVYLSPENKGFEVKALLTEAQNLKDGAEHPLPW YPPICVTLLTINEYDEKTYDNLKSVGHPGRTKVPLKMYDPSMRKTLLSHVNGGTVTEEEVGQRILSAIKQEVGARW VLFNLAGLYW RIVGNNYHGIECIRRSLALAPDEYADVPLVNMANILYKW GRYDDAIVLMKDALKINDAEPDSNFLMANLLW VTKNYSGAISHYRTVLDIIPEHQEAFDSLRAIKCLQKFLIKEQSVAPIEVPSQPVGNSCQQKGVANKNQQTESRVICKTENGEEKCIIETRMRGKSGECNGHCTQTCTITPIKVESSCGGPDLAVDTSNPVQCHQKGSQCGEAGEELLFTNDFTNKLDEVSDHYEKKGICNGEDCNTLRVQCLIPMKTHSGLVAHVITPPKLFVRPLSLHSTQCMTEQKKPHIKLEFIDGVLHQKLIFVQDANDIHVEDNECIIFNDGIKSPGCDQAQYRSYSEELARSNINVEFRYVGDESPVEKDKKNHCSKTPNSPGVPSDEIPYSDISDGILQSLNGDVEPSEVVGHRIALTLQQNPKSW VAASAAGLYW RVEGDFTKALDCYRLSLSYAPLEMADLMLLGYANILVQGGYYSNAIMVEDLALDIQSTLPIHYFTLGNIYAALGKWRTAVSFYEATRFYQEDFKPAVDRLRAIHCENIA* Lottia Protein Model: jg i_Lotgi1_165259_fgenesh2_pg.C_sca_50000100 NR Annotation: hypothetical protein CHLREDRAFT_144736 [Chlamydomonas reinhardtii] gb|EDP05543.1| predicted protein [Chlamydomonas reinhardtii] NR E value: 6.00E-23 Sequence: MKVLLVLDICCLIGLVSVGEAVCPDNCNYNGVCDVKHSRCNCYEGFTGSDCKTDCRCNGHGTCQSGSVCKCDEGWKYSGGQCVW DCHCLNGAKCIGPGECGCVHNCKMGNCRNGQCQCW NGYKGSDCSEYDPTM* Lottia Protein Model: jgi_Lotgi1_166436_fgenesh2_pg.C_sca_58000065 NR Annotation: PREDICTED: similar to MEGF6 [Strongylocentrotus purpuratus] ref|XP_001187830.1| PREDICTED: similar to M EGF6 [Strongylocentrotus purpuratus] NR E value: 1.00E-26 Sequence: MMKILEILLCLSFFRATVQQFDCSVCKVCDNSTCIECIEGRYGISCGSFCSLKCSGRCRQSDGYCTSGCDEGYWGPGGLCDQTCSNCNPGGCVMNSGLCIAIGCRTGW YGTTCNNKCNSNCDGNCNQSNGNCNQCIPGKYGTDCQLDCSNCKDTCNKNNGVCNNGCKEGLW GTSCTKTCPNCQSSGCDIDNGNCNNPGCRIGW YGDSCNDKCNERCNDGCKQDTGYCNKCNKGVYGNVCDKNCNKGCLDGCNIDGSCKCKSTYYGTNCSKQCSSNCVDSLCNEISVGYNSCIRGCVAGYKGLYCDKQCPSSCPICNRFDGSCTECSKGFYGTECQLKCPEQCDKCKIKEGTCESCNPKYHGPVCSKRCSDKCLNGECNGVGGRCIDCIPGTFGDNCERSCSNGCIGDTSSTTCDRNGSCISGCINRIYGEKCNLNCSKNCYGSCNRSNGYCNSCLSGYFSDNCNQKCSIHCNGDCDRSTGNCGKCNEGYFGDNCNQNCSINCNGGCQKSSGSCLECNAGYYGDYCKQQCNDKCINNECLRNGQCRFGCMNGKTGSQCDGNCNSNCTICLQTDGNICKECKPGLYGISCDKLCNNTCKKVNSKPACNITTGKCTTGCIDGYKGYYCQDTCLDNCISCNNNQCTSCNTGW YGNQCQTKCNSNCINRCDQATGDCLDGCIDGKSGKHCKQEIG* Lottia Protein Model: jgi_Lotgi1_168805_fgenesh2_pg.C_sca_79000073 NR Annotation: PREDICTED: similar to plexin A CG11081-PA, isoform A [Apis mellifera] (a neuronal semaphorin receptor that controls axon guidance) NR E value: 1.00E-121 Sequence: MEGGIRLQLFLLVSLICHTQCIKILHSFRDPGGNILQKLVIDDQTGNVFLGALNRLHKLDPDLNIVQSASTGPRLDNVECPPPLLPCDKPKTQLNSQIKGLVIDSASNSLILCSSLFHGSCQTLALNNITNVKKFFHKPLVPNDHHSCYMFLAPSINNTQALYIATAYSDLGDAAYRDLVPSISSRSLDNLDFIHRDTEGSTKLEILKEYRESFIVRQLY

216

GFQNGGFVYFITLQRENPGSQKIVSRINRLCQQDRYFRSFVEIPIKCQKSDSDSYDVVQNAYFTNGKLYVIFSNTVSSAGAKKSTFCEYDIQDIDLEFNETVRNCYKAVGNFGPEHYKKIEPCPPLIFGSVDFCGESGEW KDYSAIEGSKPISATPLHFFTSDKPTAIFVHPEGNHRYAYIGTQDGHVIKLRFENGQVNQLFKITHSVGDIILQITNHPQSNLLYILGSHKLSVLSLNHCDDLTSCDECMNDGDETCGW CVMDNRCTTKELCQSSVITPPW LSRTDQSCASISSLQPSSLSYKKFINGGDSRQIKFQVNKLKFESNRNLNCLFINGEKHDNTMAILDQKENTITCPLPRQLPTIPQGKDHEDLELQFHVEGKMIVKRDVSVFDCQGNNNCTSCSTSQFNCHWCPVSHSCVEQSESCPQGDSIKVNDRCPRLETAASDTDILVHSGELKTISVQVRALEANQRSNLKCHFDLSGQVQTVSAKISSSTLTCETIKLAYADDIPYIVAGFKVTW GQQNHPLDNPQRMNVRIYKCASMVTNCGKCLSLDVEYECGWCEDQCSLQKKCQKSWLDQSETCPNPQILRYSPSKGPVKGKTEIQVNGINLGKEISDILGQVTVAGLPCDVMQEHYEPSSSFKCEVKGVTSAKSGTVKVVVNNKYTAESDTVFEFVDPVLNHVDPKQGLMSGGTRVLIKGNNLDVGSDTQVIVGGNSVPVIK* Lottia Protein Model: jgi_Lotgi1_171167_fgenesh2_pg.C_sca_110000033 NR Annotation: No match found NR E value: Sequence: MAISHDWSFTLLW LIWIFTLLFSDSYGKRTQNYYMEGLDCGDSKHIGGATVYSNFRGDVSTVYGNDIECQMTFKAENKDWRLMLRIIELDIPDRTSTGLCNDALYVYDESSIYARAMEEANGNTGLCGNILPPTLYSTGQYLTVHFSVSLQNQKDIV* Lottia Protein Model: jgi_Lotgi1_171718_fgenesh2_pg.C_sca_119000006 NR Annotation: PREDICTED: similar to orphan G protein-coupled receptor HG38 [Gallus gallus] NR E value: 1.00E-15 Sequence: MAPIYLTFIASLLLASSTFCEQLCLEGCKCEDHPSEKLGPHLMIDCSNLGSSSVPRDLNKFQNYGRPMHLKLSKNIIARVSKADFPENLPIIALDFNQNRGVMITDDAFMNIKGLRYLGLNDISMPFTDTMKYLKGMTKLRYLYLNKNYQFGEGLVPVSLFSGLELKLKDLSLHDCYLKGIENGALANVVITGSLDIGANQLDHIPEEVKTLKNLTKLDVSQNSIKSIPSNTFEGMSKLRTLSLSNNYINGQSFKTDSFNGLKLKELRLEKSELNTIPSAALSSLSSLKILKLNNSPIKTIPKHSFNGSYCLEVLDLTNVLVEIENTHLDGLESCLQKLYLNNMQIREIPKFFKDFKVLNYVSLSGNSISEIKMDDLVGTTITELDLTENPLHRIEKGSFEEFPHAISLIISDTRLSNLSFVRDYEYGTIKTIKLGNVLPMCDCELAFAEYRVLEIHGNCNFNNTKNVAISTDEFMSVTSHCWPDGKMPQKKDVWAVVGDNSAGTFSTSILSYFCVFLFFKVLNIV* Lottia Protein Model: jgi_Lotgi1_172278_fgenesh2_pg.C_sca_130000017 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO35011.1| p redicted protein [Nematostella vectensis] NR E value: 2.00E-26 Sequence: MGVKLLFLGICLIYLVLDLHDAEVTAVSDRQAKLKEKDAREKLQQELSKNYAVKQRRRKKKKFRDPRTRGKSSKVVNRGGAPKVQNGDDGGDDPHIGNRENYLQKYFKGKMW GIRDKKKAPKGNW RKKLRKQKHKKKEKRQNMKKKEKSKRTKVKKFKKKRKKHKKRRRGKRSTDENEDDPGSDQEHTHRTKRYAITDQERMW DYGVVPYSFSSDVTDVMARTYRGVFDALEKRTCISFVEW DGTESFYSNRSLNHMVHLNFVDQGGCWAYLGRYTYRTTNIAQDISCCYVGRTCLHEIMHSLSMEHEHTSPLRDGW LRVNW DNLNEDGQFQFSVIDPLMLGRESW GFDIKSFVSYGTTEFTKNGMMGFQPLMPDFYPTGNSYW QPFKEVQMNYDCFGIKCSGMEEPVCENDGYPGYYEGECGCVCPPGLDPATNCASIKPTDPIRGW PPSSFGLPEPIEGCPAGFQLGSLTQPLAGDSEVSSSSHINAGFENSTAEFGFCLKDTASQTEGGLTEWPRGQYCIHSYSNACPPGFQEGLITYDDSVNATRTILTESPLPDGSFVNNTELW YCCRSDGMVQLDIKLPNTESFILYRHGTAECQNVEGMYMFPEYYSLMIEGETIRSISVYQTLGSVPYNVLDGNELFVYYCYYSPLNKDCGEVIHLGPENPTHDIMTPNYPFDYPLNTECNW LFVAPEGSEVM LNFNDFDVEGDDFDCFDYVTIKRGLPGIPGVSYCGTEFKAAIKSIDRYLMITFTSGSEITYTGFHATGRLLDVADLCYNPDDFGRTYNGIHNYAEDFTECLPW VEMAHCDTNLFNTEDAFDNLDGNYCRNPGARYDSPWCYTEKENCTIKYCDVCQERTIYDNFDDCEELIAEHPNFCLDGDHHHYGCLATCIEYGHITIPEKSNRAQDVSCPVPDDIVDADPIVHDVTRYNVGDSLAYVCSNGTATKMRRCLTDGTWTDIEYVCGGCGDEMKSDTRGTCYAYVAEEVRFVEAEEYCIEKDWDVASAQTEDAFNFLVQYRSDMGSDGKQVW LDLYEDPVGVW SW GDESLVQD

217

GFTNWRSTYPVSGPIETDGNNW YRCATMVGTGQSSVWNQVACDNKW YKAHYICQTMESEREICADRKSSCTRNLIDIPLLCSQYQSYAHEVCPYTCGICDVENADSCTVTADFVVGLNLVSGGSTPFSLNPGESFTTQCPAGQICTQNCQHFSRACRRSGEVTGATPVCQDADSVPTLVNNIERIPRLDTSEARRIYQGDNSYMNITRSGEITKWMTLCENDGIVHLMAFKYW SQDRYFKVMGVNQVECKAGRRMTW EIPEGERIQVDPSMNIGLMDWNGGCIPLYECFASEYPDLNKIYHTMAESQDEVALNKLVWMRAGPTCYFFSLNAEISPVGYIAPATTQLTTPSTTTEEPEPEPEATSTSSEDTSESTDMTTSSGDVSTSSTSTSAGNTESSSTGASRSSPSTDAFVSEDTTSSVW QRTTTHSVVITTPRRTINEDFIKSFIKKKKKKRKWWKNRGKKTDKTRKRKNKRRKKAQKGR* Lottia Protein Model: jgi_Lotgi1_172798_fgenesh2_pg.C_sca_142000011 NR Annotation: gb|AAA30047.1| calcium-b inding protein [St rongylocentrotus purpuratus] NR E value: 7.00E-07 Sequence: MRADSIQVRGLKALVLILLVLYEGHTKETCRTGVKTDKNLRGVNLDKTSGLPFYW FDLHRASDGYHSEYTYTFRLHSSSEASPFSDAVLQVETANNCGTGDLVVNTTFLYQPRNCPKMIMTEYKKPMTNVPFKWRAPKCGCIKFRATVIRNGVTYFYDDENISDGNLVKTVCPHGVRVAEQLVEVDEKTTDSTTKTLSTVTSKMDRAALLDLLCKITSTLNNLELLKRPEFIQRRKLQNSSLVKLLNIELALEQRKADISSCCHKKGEDKTICFDNVRRYRIDQLCGDGQPRIPFVEQKRNYMRKRESECCW RIGQPRYQCFSKSSEQLTEYSVDYSLDESDPANDIADYPKEVERYEMASNTKFFSPPPTLKPDNQNIETLTKLEETTTPTSVTKSVTLPPIRQPSARPHRQTTPTSVLTHHRQRPVPAVKTKLRQSRTVGQGDMGYNLRKQAKYTRKTTNLRHSASSVEITVNTLKQKLWKSSLKLECCQQGRIFAAKTGRTDVW DRCRSAAKDFRSSVVRGKKICSNTFQKCCVEQFITATAKASTIPKPNTPKGSPSTKQLVTTTSLRTNIKKENYRRAPLDVDSSREADEKYASMESKAEDDLMEQDDKASAEDAGEHVDDDGDDVSDDSDNDDQGEDGDQDEDDDQDDDDKDGDVDDDKVDEINNDEKNRKRKFIDYPREDPSADTFHNLQTKRKSTPNTDIAHRDSNHRNLKIESAKRKRNRRRHNRKLRRRKHKSD* Lottia Protein Model: jgi_Lotgi1_173484_fgenesh2_pg.C_sca_161000006 NR Annotation: No match found NR E value: Sequence: MRLHYLVTVLVVLILIDVGEGQDNISKCLELPSVKEIVTRYICPKPDPCATVDCNCNGKCVDGKCQCKNGFFGEFCTALTSLIPKVAGFVSNNKSLISSEAQTIGSIAKAGVSKKSKELKELQQIRKLRNRRLEEKKGKDFKDIQKSLGSLLGFPWAKYPGEKHLQDHNYTGPGTRLDLRLDENDKPKPGGNETFTFDFISIMLGKKQFE* Lottia Protein Model: jgi_Lotgi1_176704_fgenesh2_pg.C_sca_2718000001 NR Annotation: PREDICTED: similar to rCG46800 [Nasonia vitripennis] NR E value: 6.00E-09 Sequence: MLTIRVLGVFAVIVLAFVLVESDVELCEDDVNVCHGELPANAMCSGENNKTKRCQCKKGYKMSLDIDCTSNGSVFSCQDIDECATNNGSCEYKCQNEPGSYNCSCPEGSELQGNGLNCGPSGP Lottia Protein Model: jg i_Lotgi1_192261_estExt_Genewise1.C_sca_450209 NR Annotation: conserved hypothetical protein [Aedes aegypti] gb|EAT46740.1| conserved hypothetical protein [Aedes aegypti] NR E value: 1.00E-128 Sequence: MTLYITSLVFFLFINGTLQVKNRSSKVADRCFCKLNGEIDDCSCKVETLSVLNNNKIYPRIKSLLSRNYFRYFKVNLKKKCPFWHDDSRCALKDCHVDTCKEEEIPSAIKKGSSAYRYSEEAQKEESCAEEKELSALNTTISDESIQAFKDW KEHDESQDLFCDIDDESSADSEYVDLLLNPERYTGYKGESPHRIW RSIYQENCFKPQTEYIYGPSKSSSPLCLEKRAFHRLISGLHTSINIHLCADYLYQENLGYGMKAHWKPNVAEFQKRFDPQTSNGEGPQRLKNLFFTYLVELRAIAKAAPYLQEEDFFTADSDEDKDVRQGIDDLLNIISTFPDHFDESKLFAGNPNEAKLLKDEFRNHFRNVSRIMDCVGCDKCKLW GKLQTQGMGTALKILFSGDDIGPDSTVNAQQKKHFQLTRSEIVTLFNAFGRLSKSIHSLEMFKSLLSKS*

218

Lottia Protein Model: jgi_Lotgi1_197084_estExt_Genewise1.C_sca_820083 NR Annotation: glucose-regulated protein 94 [Crassostre NR E value: 0 Sequence: MKRFIVLGILGFLFIAGAGLLNVQAEEVVTEPDVVEDIGKSREASRTDDEAVQREEEAIKLDGLNVAQMKELREKAEKFAFEAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRFVSLTDKSAMAATDELSIKIKADKASHVIQITDTGIGMTKDDLVKNLGTIAKSGTSDFLSKLGESKDQAQMSDLIGQFGVGFYSAFLVADRIVVTSKHNDDDQYVW ESDSSSFSVAKDPRGNTLGRGTMITLHLKEEAHDYLEESTIRSLVKKYSQFINFDIFLWASKTEEVEEPIEEEEEEKKEEEKKEDEDAEVEEEKEEKPKTKKVSKTIWDW ELINNVKPIWTRKPSEITDDEYNDFYKSISSDYEKPMTKIHFTAEGEVTFKSILYVPNSSPSDTFQNYGKQMEQIKMYVRRVFITDDFEDMMPKYLSFIRGVVDSDDLPLNVSRETLQQHKLLKVIKKKLVRKTLDMIKKIDKDTYEKFW KEFSTNIKLGVIEDTTNRTRLAKLLRFSSSNSDTELTSLAEYLERMKEKQEHIYFVAGHNRAEVEKSPFVERLLKKGYEVLYLTEPVDEYCIQSLPEFESKKFQNVAKEGLKLDDSEKAKERKEATEKEFEPLITWLKDSALNDKIEKATISERLASSPCALVASQYGWSGNMERIMQAQAYAKAKDPSQEFYATQKKTLEINPRHPLIKELKKRVEANKEDETAKDLAVVMFETATVRSGYRITDTAGFAQRVENMLRLSLEVPLDAKIEEEPEEEPEEAEEEKTEEVEAEAEEEAEEKKSEHEEL* Lottia Protein Model: jgi_Lotgi1_202874_estExt_Genewise1.C_sca_21760001 NR Annotation: pred icted protein [Nematostella vectensis] gb|EDO37140.1| predicted protein [Nematostella vectensis] NR E value: 2.00E-54 Sequence: MMKLFVILALLAGQSLADIYLHNPRGSNDRLNEKSAQRANANRLFDSQNNNRGGYNVGDVTSAPHGKDASKQYKMAYFQSEADAETILTVEW YNQHGCGGNEDDNPQKQNCRLVLQYMCQPEGTTEDVLRNGVVTNTQDYNRPPNSNYNLANRNSRKNNNVKADRGLQESW DW YEECFVRERNKGLFTADQNLRGNNGLGYSSAIYTR Lottia Protein Model: jgi_Lotgi1_203670_estExt_fgenesh2_kg.C_sca_170029 NR Annotation: PREDICTED: hypothetical protein [Ornithorhynchus anatinus] NR E value: 1.00E-142 Sequence: MKLSVFLSLLFLTAVWADTPANCTFDDIKGTWTFFIGSNGHDNTVNCSNMGNPKSQLQVTLYFPDVAVDEFGNKGFWTIIYNQGFEVVISGRKYFAFSMYEQSGSTVSSICDKTLPGWSHDTFNRDWACYYGKKITSVPPKYYKKNKPHRLFKLNYDYINNINKQQKLWTATVYPEYEGKPMRELLNKAGGPASQVFGPIKAAPVTW EQQRLSETIPEQFDW RNVNDNNYVSPIRNQESCGSCYAFSSMAMNEARVRIMTNNTLQPVFSPQDIVECSEYSQGCAGGFPYLIGGKYAEDFGLVEEKCNPYKGQDHSCSTDKTCKRQYATSYRYVGGFYGGCNDALMKMEIYRHGPVVVGFNVTDDFFHYKSGIFIHTGLTDRYNPFEVTNHAVLVVGYGTEKGVKYWIVKNSW GEKW GESGYFRIRRGTDELGIESMAVSSTPIF* Lottia Protein Model: jg i_Lotgi1_203811_estExt_fgenesh2_kg.C_sca_240005 NR Annotation: predicted protein [Nematostella vectensis] gb|EDO37140.1| p redicted protein [Nematostella vectensis] NR E value: 0 Sequence: MMKLFVILALLAGQSLADIYLHNPRGSNDRLNEKSAQRANANRLFDSQNNNRGGYNVGDVTSAPHGKDASKQYKMAYFQSEADA ETILTVEW YNQHGCGGNEDDNPQKQNCRLVLQYMCQPEGTTEDVLRNGVVTNTQDYNRPPNSNYNLANRNSRKNNNVKADRGLQESW DW YEECFVRERNKGLFTADQNLRGNNGLGYSSAIYTRQNPNGNRRGYECPEERDYFPYW HPTQWKDIAILAENETLCNPLIAESFNNKPYGACLQKFPNSQVRKPSRYNNKEECEENGLDW VAFHNYLEKDKTASTQAACQAKSTNNIEYTWAIPYDSTTFTAECLVKVPSPQCQEAPWSRSNHLGNGIGGEMNTVQWTLPHFPSGQVQNCVMRMRYNISTDDYDPFNTDSEFNGADNSPVTNNPYVDIGVGRGPLRLAINTAQFGRVFQDRTHIFQLKPRPDDIKDFRIFNVNVRGKRGNIVQVYPAVEYDFTPTDLEVTENDLVHFQWTGSNTHNNGRPGGDGQTGDAGEGKSGSDRHNILQMADRNENFPLPFEKTNMWDN

219

AEIKWIYHGKNDVSARDLALNMASSGYYKCVSATDCPAESFKDFLVDTKDKMQVTLDNTPASYPGVVLKFKQGKYHYMCTRNNNFTNRSQKATITVAA* Lottia Protein Model: jgi_Lotgi1_204607_estExt_fgenesh2_kg.C_sca_690020 NR Annotation: ctg4a [Aedes aegypti] gb|EAT44181.1| ctg4a [Aedes aegypti] NR E value: 2.00E-45 Sequence: MAFMFFQTLLIIVSIGYLHADDSDGVQEATLCEVCRILAEELQLRLDETGKSKEVIETGHGLDTKKKKKYNFSELRLIEALQEPNICDRILEYSVHKEKTGIERFAKGRSQTMEALHGLVNKGVKVELGMPHEMWDKPSVEITQMQRKCYALVEEYDEDIEDW YYNHQDQPFLDYFCRNLVLNPQNNECLDEDMRSQNEGSSSEMKGDKGKKEEL* Lottia Protein Model: jg i_Lotgi1_204770_estExt_fgenesh2_kg.C_sca_820009 NR Annotation: unnamed protein p roduct [Tetraodon nigroviridis] NR E value: 3.00E-35 Sequence: MAATTHKMFLVACLVFLPIFASADTCLGPSVSAETYTTSEVTVSTETVFIAQFTLTCKNGLQNVNLYAEVAGRMIPAVKTSKPNEYQISITDEYKALRSGTYEAKFYDEESFSALKKAKRNNEDSNSVTPLFSINISHSGLWQGSYIQSEFVAACVAILVWW LAFSAKSKLSP* Lottia Protein Model: jgi_Lotgi1_98330_gw1.10.599.1 NR Annotation: hypothetical protein NEMVEDRAFT_v1g147894 [Nematostella vectensis] gb|EDO28414.1| predicted protein [Nematostella vectensis] NR E value: 5.00E-06 Sequence: TVCSLGTHCLSITVCSLGTHCLSITVCSLGTHCLSITVFCSLGTHCLSITVCSLGTHCLSITVCSLGTH CLSITVCSLGTHC

220

APPENDIX A MODERN VIEW OF ANIMAL PHYLOGENY

A. A conservative consensus of the current state of all animal phylogeny. Phyla for which a large amount of data (genome or EST projects) is available are indicated in red. The dashed lines indicate controversial relationships. [Image taken from Philippe, H., and Telford, M.J. (2006). Large-scale sequencing and the new animal phylogeny. Trends Ecol Evol 21, 614-620.]

221

B. Recent work has improved the resolution of the animal tree of life by sampling from 29 animals belonging to 21 phyla. Phylogeny is based on collected ESTs and a maximum likelihood analysis. [Image taken from Dunn, C.W., Hejnol, A., Matus, D.Q., Pang, K., Browne, W.E., Smith, S.A., Seaver, E., Rouse, G.W., Obst, M., Edgecombe, G.D., et al. (2008). Broad phylogenomic sampling improves resolution of the animal tree of life. Nature 452, 745-749.]

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APPENDIX B MOLLUSCAN CLASSES

The topology of the molluscan phylogeny is still highly debated. There are two prevailing mod els, A) Aculiferan and B) Testarian models. Recent work (Sigwart and Sutton, 2007) incorporating both Paleozoic and extant molluscs supports the monophylyl of Aculifera. [Image taken from Sigwart, J.D., and Sutton, M.D. (2007). Deep molluscan phylogeny: synthesis of palaeontological and neontological data. Proc Biol Sci 274, 2413-2419.]

A

B

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BIOGRAPHICAL SKETCH

Jinnie was born in Tampa, Florida in 1984. She graduated from Land O’ Lakes High

School in 2002. From there she went to the University of Central Florida and graduated with a

Bachelor of Science degree in micro and molecular biology in 2006. Following graduation,

Jinnie began her graduate work at the University of Florida with a focus in neuroscience from a

bioinformatic and molecular biology perspective. She completed a Master of Science in medical

scienes in 2009.

IDENTIFICATION OF NEUROSECRETORY MOLECULES IN APLYSIA CALIFORNICA

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