proteomicsByRC7801A27

HISTORY• The term "proteomics" was first coined in 1997 to

make an analogy with genomics, the study of the genes.

• Proteome = protein + genome • The word "proteome" is a blend of "protein" and

"genome", and was coined by Marc Wilkins in 1994 while working on the concept as a PhD student.

What is proteomics ?• Proteomics is the large-scale study of 

proteins, particularly their structures and functions.

• It include large scale characterization of proteins .

• Proteomics is very important because it helps in study of proteins which are responsible for phenotype of cells.

• Aim of proteomics is to create a 3-d map of cell indicating where proteins are located.

• The proteome is the entire set of proteins expressed by a genome, cell, tissue or organism and proteome is dynamic

proteomics

Expression proteomics

Structural proteomics

Post translational modifications

Protein-proteininteractions

Proteome mining

Functional proteomics

Protein functions,analyse protein network,identifying novel proteins

Protein complexes,organelle composition,subproteome composition

Identifying disease specific proteins ,compare protein expression of different proteomes,for instance cell of cancer and normal cells can be analysed

Drug discovery,Drug targets

Proteolysis,glycosylation,phosphorylation

Application of proteomics

Creation of novel and identify neutraceutical proteins

Biomarkers to diagnose disease,identify new drugs

Protein engineering and infinite functions which proteins perform

PROTEIN BASED PRODUCTS DISCOVERED

BLOOD PRODUCTS AND VACCINESTHERAPEUTIC ANTIBODIES AND ENZYMESTHERAPEUTIC HORMONES

REGULATORY FACTORS LIKE CYTOKINES INCLUDING INTERFERONS,TUMOR NECROSIS FACTOR. ALPHA INTERFERON IS USED FOR HEPATITIS C THESE DAYS,BETA FOR MULTIPLE SCLEROSIS AND GAMMA FOR CHRONIC GANULOMATOUS DISEASE

INDUSTRIAL ENZYMESFUNCTIONAL NON CATALYTIC PROTEINS LIKE KAPPA CASEIN HELPS IN MICELLE STABILIZATION OF MILK PROTEINS.THESE PROTEINS RESPONSIBLE FOR AROMA ,FLAVOUR OF SOME BEVERAGES

Neutraceutical proteins=proteins with therapeutic values

for example

Whey protein

Infant food formulation is done

Lactose free milk,Curd used as a

probiotic as it contains beneficial bacteria colonising the intestinal tract

Therapeutic uses of whey protein

The search has shown that :It results in the elevation of a tripeptide called glutathionine in cells It is a reducing compound leading to detoxification of xenobiotics and protection of cells against oxygen intermediates and free radicalsThe constituents of whey are:

Whey components

A-lactalbuminB-lactoglobulin

Bovine serum albuminImmunoglobulins

LactoferrinsLactoperoxidase

ProteaseFree amino acids

UreaGlycomacropeptides

Growth factorLactose

Sialic acis lactic acidSodium

Potassium calciummagnesium

ChloridePhosphate

SulfateCitrate

Heavy metalsMilk fat globule free fat

Lipo protein

Novel proteins

Since proteins are the main molecules which provide stimulus to immunity so proteins are engineered having minimum deleterious effects.The specific sequence of amino acids in the protein which stimulate immune response are known as epitopes.Recombinant vaccines based on epitopes are made with optimal design to make a safe vaccineThese days subunit vaccine against Hepatitis b virus (hbv) infection

Want to be a good athlete!!!!!!!use

And BCCA BCCA are branched chain amino

acids released during exercise from muscle the carbon part used as a fuel and nitrogen part makes alanine which goes to liver where it turns into glucose for energy so athletes these days take BCCA before and after exercised

conclusionStudy of proteins is very important because:•They have lot of functions as mentioned in ppt•One main important reason is that relation between proteins and genes is non linear

•Why ?•Because mRNA analysis does not provide relation about protein expressions •Formation of mRNA is only first step in protein synthesis now this mRNA undergoes various post transcriptional modifications which leads to :•Generation of various proteins from a single gene •So more proteins are their and their expressions should be studied to explore new discoveries

thankyou