Proteomics: 2D Gel

Proteome analysis 장치 (2D)

Root Proteome Nodule Proteome

Table I. Proteins identified in soybean root and nodule through MALDI-TOF/MS & LC-MS/MS

Spot No.

Calc. pI/ Mwa

Z-scoreb / Mascot scorec

Identifyd

Species (NCBI accession No.)

Theor. pI/MW

SCe (%)

StatusAnaly-zerf

Ⅰ. Primary metabolism

sr038g 6.19/41 1.65/ -Cytosolic malate dehydrogenase

Cicer arietinum(CAC10208)

5.9/35.82 24 . m

sr150 5.98/42 0.99/ -Malate dehydrogenase, cytoplasmic

Beta vulgaris(Q9SML8)

5.9/35.82 20 5d-up m

sr151 5.98/42 1.1/ -Malate dehydrogenase, cytoplasmic

Beta vulgaris(Q9SML8)

5.9/35.82 20 5d-up m

sr160 6.42/41 0.85/ -Malate dehydrogenase, Mdh-2, mitochondrial

Glycine max(T06326)

5.6/27.54 15 . m

sr161 6.42/41 1.6/ -Malate dehydrogenase, Mdh-2, mitochondrial

Glycine max(T06326)

5.6/27.54 21 . m

sn125h 6.41/38 -/ 741 Malate dehydrogenase

Bradyrhizobium japonicum USDA 110(NP_767096)

5.88/34.1 44.7210d-new, 13d-up

t

ssn002 6.00/43 -/ 62 Malate dehydrogenaseGlycine max(AAD56659)

8.23/36.1 8.11 10d-new t

sr101 5.40/40 0.23/ -pfkB-type carbohydrate kinase family protein

Arabidopsis thaliana(NP_180697)

5.3/35.43 18 . m

sr112 5.30/40 0.89/ -pfkB-type carbohydrate kinase family protein

Arabidopsis thaliana(NP_180697)

5.3/35.43 24 . m

단백질의 질량 스펙트럼

단백질

효소

질량분석기MS spectrum

Peptide sequenc

es

Proteindatabase

Protein id

A

B

A B

Proteomics: MALDI-Mass

Mass spectrum vs. database

Mass spectrum

database

Protein A

Protein B

Protein C

탄뎀 질량 스펙트럼(MS/MS spectrum)

단백질

효소

1st MS 2nd MS

MS spectrum

energy

Tandem MS

spectrum

Peptide sequenc

e

Proteindatabase

Protein id

탄뎀 질량 스펙트럼Peptide fragmentation

H2

NCOOH

R1

C

H

O

N

H

C

R2

C

H

O

N

H

C

R3

C

H

O

N

H

C

R4

C

H

x3 y3 z3 x2 y2 z2 x1 y1 z1

a1 b1 c1 a2 b2 c2 a3 b3 c3

2D data 분석