; a common brown seaweed found in the Medway and around the British coast

The CO2 Micro-algae Biorefinery

Stakeholders are invited to participate in the Dunaliella D-Factory Innovation Platform: Owners of salt-water Dunaliella cultivators Biorefinery extraction technologies Land planners, policy makers, investors Dunaliella research community Pharmaceutical sector Nutraceutical sector Food/feed sector Prebiotics

Contact: D-Factory leader: Pat p.j.harvey@gre.ac.uk

Dunaliella resources: David d.bailey@gre.ac.uk Algal strain identification: Declan/Richard culturecollection@mba.ac.uk

Dunaliella cultivation: Ami amiba@bezeqint.net PBR manufacture: Vitor vitor.verdelho@gmail.com Harvesting algae: Herre herre.hoekstra@evodos.eu

sCO2 extraction of algae: Nadine nadine.igl@nateco2.de HPCC extraction of algae: Guy Guy.Harris@dynamicextractions.com

Use of membranes: Joao jgc@fct.unl.pt Modelling D-Factories: Antonis akokossis@mail.ntua.gr

Formulation: Karin karin.persson@ri.se Economic enquiries: Paul p.goacher@blueyonder.co.uk

Sustainability: Guido guido.reinhardt@ifeu.de Publicity: Roberta r.modolo@insrl.eu

This document has been produced with the financial assistance of the European Union. The contents of this document are the sole responsibility of the University of Greenwich and can under no circumstances be regarded as reflecting the position of the European Union.

13 partners 8 countries €10m: producing suites of compounds from Dunaliella to meet market requirements

New strains of halophytes

The D-Factory Plan

Grant agreement no: 613870 A Collaborative Project funded under KBBE.2013.3.2-02 by the European Union

University of Greenwich UK

Algafuel SA PORTUGAL

Dynamic Extractions Slough UK

Evodos Raamsdonksveer THE NETHERLANDS

Hafren Investments Ltd London UK HI Instituto de Biologia Experimental e Tecnológica PORTUGAL

Institut für Energie- und Umweltforschung Heidelberg GERMANY

National Technical University of Athens (NTUA) GREECE

In Srl, Udine ITALY

Nature Beta Technologies Ltd (NBT) ISRAEL

The Marine Biological Association (MBA) UK

NATECO2 GmbH & Co. GERMANY

RISE Research Institutes of Sweden SWEDEN

New isolates of halophytic algae are being profiled for use in D-Factory sites

Cultivation in raceways New raceways are equipped with novel harvesting

technology to concentrate fragile cells intact

Cultivation in photobioreactors Photobioreactors resilient to high salt concentrations are

able to control cultivation parameters on demand

Pilot raceway at NBT Eilat

Evodos dynamic settler (Spiral

Plate Technology)

New modular salt-resistant PBRs

Processing cell extracts with

membranes Membrane bioreactors, FPLC & LC-MS recover

peptides, proteins

Processing cell extracts with

ScCO2

Ultrahigh (1000 bar) pressures with CO2 extract carotenes, xanthophylls, PUFA’s, vitamins, chlorophyll

Processing cell extracts with

solvents using HPCCC

4.6l -18l CCC machines recover lipophilic compounds based on size and solubility in solvents

Pilot membrane unit (2.5–7.5m2; spiral-wound; cross flow)

FPLC recovers proteins, enzymes

Biorefinery of the future

Global Dunaliella production

Dunaliella

Asteromonas