Genetic engineering - كلية الطب · 2020. 1. 22. · Genetic Engineering (g.e) 1. It is the...
Transcript of Genetic engineering - كلية الطب · 2020. 1. 22. · Genetic Engineering (g.e) 1. It is the...
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Genetic Engineering
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Genetic Engineering
• Genetic Engineering:
The development and application procedures, and technologies that allow you to directly manipulate an organisms DNA
• We can manipulate DNA and genes
to alter organisms or make them
produce a product we need.
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Genetic Engineering (g.e)
1. It is the process of inserting genes of interest into
specific organisms for either a medical or scientific
benefit.
2. Gene therapy is the process of inserting a missing
gene into an organism.
3. Bacteria are often used as the factories to produce a
protein from a cloned gene. This has led to the
production of human insulin,GH & erythropoietin..etc.
4. The gene must be cloned into an expression vector,
usually a plasmid with special features that allows it
to be transcribed & translated in a host cell.
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Genetic Engineering in
Agriculture 1. Disease resistance e.g. corn & cotton
2. Nitrogen fixation
3. Frost-free plants e.g. strawberries & potatoes
4. Tomatoes with a long shelf life deactivating the
gene in tomato which produce ethylene.
5. Increased milk production giving cows bovine
somatotropin (BST) “growth hormone”….
6. Good predator attraction strawberry gene on
mustard plants produces a chemical attractant for
predator mites that eat the herbivorous spider
mites.
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Fig. 13-18, p.349
Transgenic tomato plant : Recombinant DNA methods have produced plants
that resist defoliation by caterpillars, with longer shelf life.
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• Genetic engineering: Changing the DNA in
living organisms to create something new.
• This organisms are called Genetically
Modified Organism (GMO)
• Example:
• Bacteria that produce human insulin
• Genetically Modified organism are called
transgenic organism; since genes are
transferred from one organism to another.
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Some genetic engineering techniques are
as follows:
1. Artificial selection
2. Cloning
3. Gene splicing
4. Gel electrophoresis: analyzing DNA
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1. Artificial selection: breeders choose which
organism to mate to produce offspring with
desired traits.
• They cannot control what genes are passed.
• When they get offspring with the desired traits,
maintain them.
Three types of artificial selection:
A. Selective breeding
B. Hybridization
C. Inbreeding
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A. Selective breeding: when animals with
desired characteristics are mated to
produce offspring with those desired traits.
• Passing of important genes to next
generation.
• Example: Champion race horses, cows
with tender meat, large juicy oranges on a
tree.
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• Examples of selective breeding:
• Angus cows are bred to
increase muscle mass so that we get more meat,
• Egg-Laying Hen-produces more eggs than the average hen
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• Selective breeding occurs when you choose the
best male and female to breed.
• This allows you to fine tune and control the
traits
• The offspring or babies will then have the best
traits.
• Then you continue to breed those organism
with the best traits, those traits will be
maintained.
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Selective Breeding
• Selective Breeding: allowing only those organisms with desired characteristics to reproduce.
– How could you use selective breeding to develop dogs with more intelligence?
– Selective breeding takes advantage of natural genetic variation (the trait must already exist in the population)
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2 Methods of selective breeding
1. Hybridization: crossing dissimilar individuals together to get the best of both organisms
Ex: Killer Bees – While attempting to create a bee that produces more honey in tropical climates scientist bred a European honey bee with a African honey bee.
2. Inbreeding: the continued breeding of individuals with similar characteristic Ex: Purebred Dog Breeds – dog breeds are created by
breeding individuals with similar characteristics to ensure that the combination of traits will be passed on to the next generation.
Selective Breeding
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• B. Hybridizations: two individuals with unlike
characteristics are crossed to produce the best in both
organisms.
• Example: Luther Burbank created a disease resistant
potato called the Burbank potato.
• He crossed a disease resistant plant with one that had
a large food producing capacity.
• Result: disease resistant plant that makes a lot of
potatoes.
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Other Examples of hybridization:
1. Liger: lion and tiger mix
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2. Grape + apple= grapple. The fruit
tastes like grapes and looks like apple.
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C. Inbreeding breeding of organism that
genetically similar to maintain desired traits.
• Dogs breeds are kept pure this way.
• Its how a Doberman remains a Doberman.
• It keeps each breed unique from others.
• Risk: since both have the same genes, the
chance that a baby will get a recessive
genetic disorder is high.
• Risks: blindness, joint deformities.
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Selective Breeding • In order for selective
breeding to work, you need a wide variation of genetic traits.
– Explain you cannot use selective breeding to create a monkey that glows in the dark?
• How do new traits get introduced into a population?
– Induce mutations to develop new traits in a population
(Mutations are the ultimate source of genetic traits)
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Limitations of selective breeding and mutations:
–Selective breeding requires traits already exists in a population – we can not make new traits.
–Mutations are unpredictable and will not create the exact traits that we want. (most mutations are harmful to the organism)
Selective Breeding
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• Variation: difference between individuals of a species.
• The differences are in the genes but we see the physical differences.
• For example: Some humans have blond hair and some have brown. This is a variation among humans.
• Inbreeding decreases variations.
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2. Cloning: creating an organism that is an
exact genetic copy of another.
• There are human clones in our school.
• Identical twins are naturally created clones.
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• Eggs are haploid
• Haploid: half the
chromosomes, 23 in
humans
• Body cells are diploid:
• Diploid: two sets of
chromosomes, one from
mother and one set from
father 46 in humans.
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• This picture represents gene splicing.
• However, DNA is much smaller.
• Its done with high tech lab equipment since
DNA, is too small to hold or see without a
microscope.
The red piece the woman
is holding is an insulin
gene from a human
being. It is being
combined with DNA from
a bacteria.
Creates recombinant
DNA, something that has
never existed before.
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Benefits:
• Insulin is cheaper
• There are no side
effects because it
is human insulin.
• We once used pig
insulin but there
are side effects
and it is more
expensive.
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How is gene splicing done?
1. A restriction enzyme cuts the insulin gene out of the human DNA.
2. A plasmid is removed from a bacteria and cut with a restriction enzyme
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3. The human gene is place into the bacteria
plasmid
4. The plasmid is placed back into the bacteria.
• The cell now has directions (DNA) to make
insulin.
• That's exactly what it does.
• Its human insulin, bacteria do not make insulin
on their own.
Plasmid with
insulin gene
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• This is called transformation: when a gene
from one organism is transferred to different
organism.
• The organisms that have DNA transferred to
them are called transgenic organisms.
• trans: means different,
• genic: refers to genes
• Genetic engineering has given rise to a new
technological field called biotechnology
(technology of life).
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1. Transgenic (GMO) animals: genes inserted
into animals so they produce what humans
need.
• Why?: A way to improve the food supply:
A. Transgenic cows: gene inserted to
increase milk production.
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B. Spider goat: gene from spider inserted
into goat.
• Goats makes silk of the spider web in their
milk.
• Flexible, stronger than steel. Used in
bullet proof jackets.
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C. Glow-in-the-dark
cats
• Scientist used a
virus to insert DNA
from jellyfish
• The gene made the
cat produce a
fluorescent protein
in its fur.
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2. Transgenic bacteria: gene inserted into
bacteria so they produce things humans
need.
• For example: insulin and clotting factors in
blood are now made by bacteria.
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3. Transgenic plants: plants are given genes
so they meet human needs.
A. Transgenic corn: given a gene so corn
produces a natural pesticide.
Now they don’t have to be sprayed with
cancer causing pesticides.
• 25% of all corn is like this.
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B. Venomous cabbage
• Gene from a scorpion tails
inserted into cabbage.
• Cabbage now produces
that chemical.
• Why? Limit pesticide use
while still preventing
insects from damaging
crops.
• Corporations state the
toxin is modified so it isn’t
harmful to humans.
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C. Banana vaccines
• Virus is injected into a banana, the virus DNA becomes part of the plant.
• As the plant grows, it produces the virus proteins — but not the disease part of the virus.
• When people eat a bite, their immune systems creates antibodies to fight the disease — just like a traditional vaccine
• Vaccines for hepatitis and cholera
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Medicinal eggs
• British scientists have created a breed
of genetically modified hens that
produce cancer-fighting medicines in
their eggs. The animals have had
human genes added to their DNA so
that human proteins are secreted into
the whites of their eggs, along with
complex medicinal proteins similar to
drugs used to treat skin cancer and
other diseases.
• What exactly do these disease-fighting
eggscontain? The hens lay eggs that
have miR24, a molecule with potential
for treating malignant melanoma and
arthritis, and human interferon b-1a,
an antiviral drug that resembles
modern treatments for multiple
sclerosis.
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Fast-growing salmon
• genetically modified
salmon grows twice
as fast as the
conventional variety
— the photo shows
two same-age
salmon with the
genetically altered
one in the rear.
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• A virus is often used to deliver DNA.
• In the movie “I Am Legend,” A healthy gene was inserted into a virus.
• The virus invaded the cancer cells and inserts the healthy gene to cure cancer.
• Worked at first but the virus mutated and became deadly.
• This is being attempted in real life.
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• Gene therapy: when disease causing
genes are cut out and good gene are
inserted.
• Restriction enzymes are used to cut out
bad genes.
• Viruses are used to insert good genes.
• Not approved for human use yet.
• Some possible side effects.
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4. Gel electrophoresis: a
technique used to compare
DNA from two or more
organisms.
Why compare DNA:
1. Find your baby’s daddy
2. Who committed a crime.
3. How closely species are
related.
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How is electrophoresis done?
A. The DNA is cut into fragments with a restriction enzyme.
B. The cut DNA is then put into the wells of a machine filled with gel.
• The gel is spongy and the DNA squeezes through the pores.
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C. The machine is plugged in and the
fragments get separated based on their size.
• The smaller fragments move further than the
large.
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• Electrophoresis
results
Separation of DNA based on
size of fragments.
Final result of electrophoresis
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• Electricity provides the energy
• Why does DNA move?
• DNA has a negative charge.
• When the machine is plugged it, its moves towards
the positive pole created by the electricity
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electrophoresis
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Your DNA is so unique its considered to be a
DNA fingerprint.
Gel electrophoresis will separate your DNA
differently from anyone else.
Nova: who done it http://www.pbs.org/wgbh/nova/sheppard/analyze.html
http://www.teachersdomain.org/asset/tdc02_i
nt_creatednafp2/