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Gel/Glc Heat 24hCTS/Gel GTA 48hGelatin GTA 48hCTS/Gel/Glc Heat 24hSevere ImflamSlight ImflamRat subcutaneous testCombination use with FGF2FGF2 (Fibrast spray) 50g/pieceCell ingrowth Combination use with FGF2FGF2 (Fibrast spray) 50g/pieceBone augmentationRat bone forming testMarked degradationSlight effectGel/Glc Heat 24hCTS/Gel/Glc Heat 24hGel/Glc Heat 24hCTS/Gel/Glc Heat 24hCyto compatibility(SEM)Gelatin GTA 48hGel/Glc Heat 24hCTS/Gel/Glc Heat 24hFGF2 stimulatedCells were attached to the surface of scaffolds CTS/Gel GTA 48h

CTS/Gel GTA 48h

Gel/Glc Heat 24hWe found that cells were attached and spread on scaffold surface.Scaffold; 886 mmCTS/Gel/GlcNAc scaffold+ FGF2 ; 50 g(Fiblast spray, Kaken Pharmaceutical, Tokyo, Japan)

Following a skin incision, a flap was made in the scalp. Decortication of a 4 mm2 area was performed in front of the coronal suture in the cranial bone using a rotating round bur under water irrigation. Subsequently, one of two types of sample was placed on the cranial bone with decortication (Fig): Gel/GlcNac and CTS/Gel/GlcNac scaffold. Scaffolds were loaded with FGF2 (50g). As a control, no implantation was performed. Skin flaps were sutured and tetracycline hydrochloride ointment (Achromycin Ointment, POLA Pharma, Tokyo, Japan) was applied to the wound. Rats were euthanized 10 days after surgery using an overdose of sodium pentobarbital and specimens were collected from the wound. Six m sections located every 300 m, including the cranial bone and surrounding soft tissue, were prepared. Sections were stained with HE and examined using light microscopy. Wistar rats were used (N=4). The experimental protocol followed the institutional animal use and care regulations of Hokkaido University (Animal Research Committee of Hokkaido University, Approval No. 10-42).Gel/Glc Heat 24h +FGF

SNBNBNBSPBNB; new boneS; scaffoldPB; preexisting boneImplantation of Gel/GlcNAc scaffold frequently promoted bone augmentation. Newly formed bone included osteoblastic cells, osteocyto-like cells and bone marrow. Residual scaffold was degraded by macrophage-like cells phagocytosis. 500m 100m 100m 100m

CTS/Gel/Glc Heat 24h +FGFNB; new bonePB; preexisting boneNBMarked inflammatory response was exhibited after implantation of CTS/Gel/GlcNAc scaffold. The result is similar to that of rat subcutaneous test. Newly formed bone was slightly formed. Scaffold was degraded and replaced by connective tissue. 500m 100m 100mHistomorphometric measurements from each scaffold. The newly formed bone area was measured in each stained section collected 10 days post-surgery using a software package (Image J 1.41, National Institute of Health, Bethesda, MD, USA). The means and standard deviations were calculated for each group. Statistical analysis was performed using the Scheff test. P-values