Gel/Glc Heat 24hCTS/Gel GTA 48h Gelatin GTA 48h CTS/Gel/Glc Heat 24h Severe Imflam Slight Imflam Rat...

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Gel/Glc Heat 24h CTS/Gel GTA 48h Gelatin GTA 48h CTS/Gel/Glc Heat 24h Severe Imflam Slight Imflam Rat subcutaneous test Combination use with FGF2 FGF2 (Fibrast spray) 50µg/piece Cell ingrowth Combination use with FGF2 FGF2 (Fibrast spray) 50µg/piece Bone augmentation Rat bone forming test Marked degradation Slight effect ままま Gel/Glc Heat 24h CTS/Gel/Glc Heat 24h Gel/Glc Heat 24h CTS/Gel/Glc Heat 24h Cyto compatibility (SEM) Gelatin GTA 48h Gel/Glc Heat 24h CTS/Gel/Glc Heat 24h FGF2 stimulated Cells were attached to the surface of scaffolds CTS/Gel GTA 48h

Transcript of Gel/Glc Heat 24hCTS/Gel GTA 48h Gelatin GTA 48h CTS/Gel/Glc Heat 24h Severe Imflam Slight Imflam Rat...

Page 1: Gel/Glc Heat 24hCTS/Gel GTA 48h Gelatin GTA 48h CTS/Gel/Glc Heat 24h Severe Imflam Slight Imflam Rat subcutaneous test Combination use with FGF2 FGF2 (Fibrast.

Gel/Glc Heat 24hCTS/Gel GTA 48hGelatin GTA 48h CTS/Gel/Glc Heat 24h

Severe Imflam Slight Imflam

Rat subcutaneous test

Combination use with FGF2

FGF2 (Fibrast spray) 50µg/piece Cell ingrowth

Combination use with FGF2

FGF2 (Fibrast spray) 50µg/piece Bone augmentation

Rat bone forming test

Marked degradation

Slight effect

まとめ

Gel/Glc Heat 24h CTS/Gel/Glc Heat 24h

Gel/Glc Heat 24h CTS/Gel/Glc Heat 24h

Cyto compatibility(SEM)

Gelatin GTA 48h Gel/Glc Heat 24h CTS/Gel/Glc Heat 24h

FGF2 stimulated

Cells were attached to the surface of scaffolds

CTS/Gel GTA 48h

Page 2: Gel/Glc Heat 24hCTS/Gel GTA 48h Gelatin GTA 48h CTS/Gel/Glc Heat 24h Severe Imflam Slight Imflam Rat subcutaneous test Combination use with FGF2 FGF2 (Fibrast.

CTS/Gel GTA 48hGel/Glc Heat 24h

We found that cells were attached and spread on scaffold surface.

Page 3: Gel/Glc Heat 24hCTS/Gel GTA 48h Gelatin GTA 48h CTS/Gel/Glc Heat 24h Severe Imflam Slight Imflam Rat subcutaneous test Combination use with FGF2 FGF2 (Fibrast.

Scaffold; 8×8×6 mm

CTS/Gel/GlcNAc scaffold+ FGF2 ; 50 µg(Fiblast spray, Kaken Pharmaceutical, Tokyo, Japan)

Following a skin incision, a flap was made in the scalp. Decortication of a 4 mm2 area was performed in front of the coronal suture in the cranial bone using a rotating round bur under water irrigation. Subsequently, one of two types of sample was placed on the cranial bone with decortication (Fig): Gel/GlcNac and CTS/Gel/GlcNac scaffold. Scaffolds were loaded with FGF2 (50µg). As a control, no implantation was performed. Skin flaps were sutured and tetracycline hydrochloride ointment (Achromycin Ointment, POLA Pharma, Tokyo, Japan) was applied to the wound. Rats were euthanized 10 days after surgery using an overdose of sodium pentobarbital and specimens were collected from the wound. Six µm sections located every 300 µm, including the cranial bone and surrounding soft tissue, were prepared. Sections were stained with HE and examined using light microscopy.

Wistar rats were used (N=4).

The experimental protocol followed the institutional animal use and care regulations of Hokkaido University (Animal Research Committee of Hokkaido University, Approval No. 10-42).

Page 4: Gel/Glc Heat 24hCTS/Gel GTA 48h Gelatin GTA 48h CTS/Gel/Glc Heat 24h Severe Imflam Slight Imflam Rat subcutaneous test Combination use with FGF2 FGF2 (Fibrast.

Gel/Glc Heat 24h +FGF

S

NB

NB

NB

S

PB

NB; new boneS; scaffoldPB; preexisting bone

Implantation of Gel/GlcNAc scaffold frequently promoted bone augmentation. Newly formed bone included osteoblastic cells, osteocyto-like cells and bone marrow. Residual scaffold was degraded by macrophage-like cells phagocytosis.

500µm

100µm 100µm

100µm

Page 5: Gel/Glc Heat 24hCTS/Gel GTA 48h Gelatin GTA 48h CTS/Gel/Glc Heat 24h Severe Imflam Slight Imflam Rat subcutaneous test Combination use with FGF2 FGF2 (Fibrast.

CTS/Gel/Glc Heat 24h +FGF NB; new bonePB; preexisting bone

NB

Marked inflammatory response was exhibited after implantation of CTS/Gel/GlcNAc scaffold. The result is similar to that of rat subcutaneous test. Newly formed bone was slightly formed. Scaffold was degraded and replaced by connective tissue.

500µm 100µm

100µm

Page 6: Gel/Glc Heat 24hCTS/Gel GTA 48h Gelatin GTA 48h CTS/Gel/Glc Heat 24h Severe Imflam Slight Imflam Rat subcutaneous test Combination use with FGF2 FGF2 (Fibrast.

control CTS/Gel/Glc Heat 24h +FGF Gel/Glc Heat 24h +FGF0

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Bone area (mm2)

Histomorphometric measurements from each scaffold. The newly formed bone area was measured in each stained section collected 10 days post-surgery using a software package (Image J 1.41, National Institute of Health, Bethesda, MD, USA). The means and standard deviations were calculated for each group. Statistical analysis was performed using the Scheffé test. P-values <0.05 were considered statistically significant. All statistical procedures were performed using a software package (SPSS Japan, DR. SPSS 11.0).

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* p<0.05 vs control** p<0.05 vs CTS

**

Bone area of the Gel/Glc scaffold with FGF2 was significantly greater than CTS/Gel/Glc with FGF2 and control.