g f College Bsc Biotech and Micro Syllabus

FACULTY OF SCIENCE

M.J.P Rohilkhand University

Bareilly

Proposed Scheme and Syllabus

For

B.Sc. Biotechnology and B.Sc. Microbiology

For the session 2010-2011

Publication Department

M.J.P Rohilkhand University

Bareilly

B.Sc. Biotechnology

MJP ROHILKHAND UNIVERSITY BAREILLY

Examination- Faculty of Science

(A) Eligibility

Bachelor of Science in Biotechnology

(B.Sc. in Biotechnology)

1. (a) The examination for the degree of Bachelor of science shall consist of three parts

(1) Part I

(2) Part II

(3) Part III (Final)

(b) A candidate who after passing (i) the intermediate examination (Biology) of the Board of High School and intermediate education, Utter Pradesh or of and Indian University incorporated by any law for the time being in force, or (ii) any other examination recognized by the University as equivalent there to, has attended a regular course of study in an affiliated college for one academic year, shall be eligible for appearing at the Part I examination for the B.Sc (Biotechnology) Degree.

2. (a) A candidate who after passing the Part I B.Sc(Biology) Examination of the University, has completed a regular course of the study for one academic year in an affiliated college, shall be eligible for appearing at the Part II B.Sc (Biotechnology) Examination.

A candidate who, after passing Part II B.sc (Biotechnology) Examination of the University completed a regular course of study for one academic year in an affiliated college shall be eligible for appearing at the Part III B.sc (Biotechnology) Examination.

(b) A candidate who has passed Part I B.Sc (Biotechnology) Examination from University or any other examination recognized by the University as equivalent to the Part I B.Sc (Biotechnology) examination of the University may also be admitted to the Part II B.Sc (Biotechnology) Examination. Provided that he/she offered for the above mentioned examination of another University, a course of an equivalent standard with almost identical syllabus as required for Part I examination of the University and has attended a regular course of study for one academic year in an affiliated college for the University.

3. Every candidate shall be required to pass in all of the following papers for Part I, II and III of B.Sc (Biotecnology) examinations:

A. Part I: (1) Bioscience I (Plant Diversity) (2) Bioscience II (Animal Diversity) (3) Cell Biology (4) Organic Chemistry (5) Biochemistry (6) Biostatistics

B. Part II: (1) Biomolecules (2) Biophysics & Biophysical Chemistry (3) Molecular Biology & Animal Cell Culture (4) Genetics (5) r-DNA Technology & Genomics (6) Fundamental of Computer

C. Part III: (1) Animal Biotechnology & Animal Physiology (2) Plant Biotechnology & Plant Physiology (3) Industrial & Environmental Biotechnology (4) Immunology & Medical Biotechnology (5) Information Technology & Bioinformatics (6) Two months training (Entrepreneurship)

4. The examination shall be by means of papers, but candidate shall also be required to undergo a practical examination. Candidates shall be required to pass separately in the Practical Examination.

5. A candidate who desires to enter for an examination under this eligibility criteria must submit his/ her application on a prescribed from so to reach the Registrar not later than 15th November, proceding the date of examination. The application shall be accompanied by an examination fee Rs. 5/- for each for three parts and shall be forwarded by the Principal of the college concerned.

6. There is a uniform pattern of examination in all the papers of for Part I, II and III of B.Sc (Biotechnology) Examination and details are as Follows

7. The minimum pass marks to obtain in each paper shall be 33.

8. The distribution of marks for B.Sc (Biotechnology) Parts I, Part II and Part III practical theory Examination throughout will be as follows as per UGC norms.

9. The university rules for Examination/ Evaluation/ Improvement Examination/ Award of grace marks etc. in the above course will be at par with other science graduate courses of the university.

(B) Scheme of Examination

Bachelor of Science (Biotechnology)

There shall be three examinations, one at the end of each year, the first being the B.Sc. (Biotechnology) Part I Examination, the second B.Sc (Biotechnology) Part II examination, and the third B.Sc (Biotechnology) part III final examination.

Candidates will have to pass separately in each of the three examination, but the marks of the three examination will be counted together for a place on the pass list of the part III examination. A candidate for the B.Sc (Biotechnology) examination will be assigned division on the following basis:

First Division 60% of the aggregate marks

Second Division 45%

All the rest in the third Division if they obtain the minimum pass marks in each paper

Note: Candidates in all papers of B.Sc (Biotecnology) Part I or II and III examinations are allowed to answer question-papers through the medium of English or Hindi (Devanagari script)

Part I- B.Sc. I (Biotechnology) (A) Theory papers

S. No. Papers Code Year Title of the Papers Total Marks

1 (BSBT/BSMB-101)

I Bioscience I (Plant Diversity)

100

2 (BSBT/BSMB-102)

I Bioscience II (Animal Diversity) 100

3 (BSBT/BSMB-103)

I Cell Biology 100

4 (BSBT/BSMB-104) I Organic Chemistry

100

5 (BSBT/BSMB-105)

I Biochemistry

100

6 (BSBT -106) I Biostatistics

100

Total 600

(B) Practical

S. No. Papers Code Year Title of the Papers Sessional Marks

Theory Marks

Total Marks

7 Practical (BSBT/BSMB-107 P) I Bioscience I 25 75 100

8 Practical (BSBT/BSMB-108 P) I Bioscience II 25 75 100 9 Practical (BSBT/BSMB-109 P) I

Biochemistry & Chemistry 25 75 100

10 Practical (BSBT-110 P) I Biostatistics

25 75 100

Total 400 Grand Total (Theory + Practical) = 1000

Part II- B.Sc II (Biotecnology) (A) Theory papers


1 (BSBT/BSMB-201)

II Biomolecules

100

2 (BSBT/BSMB-202) II Biophysics & Biophysical Chemistry

100

3 (BSBT/BSMB-203)

II Molecular Biology & Animal Cell Culture 100

4 (BSBT-204)

II Genetics 100

5 (BSBT -205)

II r-DNA Technology & Genomics

100

6 (BSBT/BSMB -206) II Enzymology and Protein engineering

100

Total 600

(B) Practical


Theory Marks

Total Marks

7 Practical (BSBT/BSMB-207 P) II Biomolecules

25 75 100

8 Practical (BSBT/BSMB-208 P) II Biophysical Chemistry 25 75 100 9 Practical (BSBT/BSMB-209 P) II

Enzymology and Protein engineering

25 75 100

10 Practical (BSBT-210 P) II Biotechnology

25 75 100

Total 400

Grand Total (Theory + Practical) = 1000

Part III -B.Sc. III (Biotecnology) (A) Theory papers

S. No. Papers Code Year

Title of the Papers Total Marks

1 (BSBT-301) III Animal Biotechnology & Animal Physiology 100

2 (BSBT-302) III Plant Biotechnology & Plant Physiology 100

3 (BSBT-303)

III Industrial & Environmental Biotechnology 100

4 (BSBT-304) III Immunology & Medical Biotechnology 100

5 (BSBT/BSMB-305) III Bioinformatics 100

Total 500

(B) Practical

S. No.

Papers Code Year

Title of the Papers Sessional Marks

Theory Marks

Total Marks

6 Practical (BSBT- 306 P) III Biotechnology 25 75 100

7 Practical (BSBT/BSMB-307 P)

III Bioinformatics 25 75 100

8 Entrepreneurship III Two Months Training 75 225 300

Total 500

Grand Total (Theory + Practical) = 1000

Proposed Syllabus for B.Sc. Part- I (Biotechnology)

Bioscience I (BSBT/BSMB-101)

M.M:100 Unit I: Cyanobacteria & Lichens

General features, taxonomic position, distribution, cell structure heterocyst, water bloom, reproduction and economic importance with special reference to Nostoc.

General features, classification, distribution, range of thallus structure, reproduction, ecological significance, economic importance of Lichens.

Unit II: Algae

General features, classification, distribution, range of thallus organization, reproductuion, economic importance with special reference to Chlamydomonas , Volvox, Oedogonium, Chara, Vaucheria, Polysiphonia, and Laminaria.

Unit III: Fungi

General features, classification, distribution, range of thallus organization, reproductuion, parasexual cycle and economic importance with special reference to slime mold, Albugo, Penicillium, Agaricus, Puccinia,

Unit IV: Bryophyta

General features, classification, distribution, range of thallus organization, reproductuion, economic importance with special reference to Riccia, Marchantia and Anthoceros.

Unit V: Pteridophyta, Gymnosperms & Angiosperms

General features, out line classification, structure, reproductuion, stelar evolution, and heterospory and seed habit. Type study Lycopodium, Selaginella, Equisetum and Pteridium.

General features, out line classification of Gymnosperms, Type study of Cycas, Pinus and Ephedra

General features of Angiosperms

Bioscience II (BSBT/BSMB-102)

M.M:100 Unit I: Introduction to Invertebrate

General principle of taxonomy and animal classification. Salient features and outline classification upto order in nonchordates.

Unit II: Phylum- Protozoa, Porifera and Coelenterata

General characters of Protozoa: Protozoa and human Diseases, type study of Paramecium caudatum, General idea of origin of Metazoan, metamerism and symmetry. General character of Porifera: Type study of Sycon. General character of Coelenterate: Type study of Obelia.

Unit III: Phylum-Platyhelminthes, Annelida and Arthropoda

General characters of Playtehelminthes, type study of Taenia, Parasitic adaptation. General characters of Annelida: Type study of Leech. General characters of Arthropoda: Type study of Palaeomon, Economic importance of insects, Sericulture, Apiculture,

Unit IV: Phylum- Mollusca, Echinodermata and Minor Phyla

General characters of Mollusca: Type study of Pila globosa. General characters of Echinodermata: External feature of Star - fish, General introduction of minor phyla and their examples.

Unit V: Phylum- Introduction of Chordata

General characters and outline classification of phylum chordata. General characters and classification of class mammalia.

Cell Biology (BSBT/BSMB-103)

M.M:100

Unit: I

Cell as a basic unit of living system, Biochemical composition of cell, protein, lipid, carbohydrate, nucleic acid. The cell theory, Ultra structure of cell, Membrane composition, Early studies on Plasma Membrane. The lipid bilayer membrane. A summary of membrane function – simple diffusion, Facilitated transports, Active transport, Endocytosis, Pinocytosis, Phagocytosis, Exocytosis.

Unit: II

Structure and function of Mitochondria, chloroplast, Endoplasmic reticulum, Golgi complex, Lysosome, Ribosome and Peroxisome.. Evolution of Mitochondria and Chloroplast.

Unit: III

Structure and function of Nucleus, Cell cycle: - Mitosis and Meiosis.

Unit: IV

Nucleic acids, structure of DNA, DNA as genetic material- Griffith experiment, experiment of Hershey & Chase, Meselson-Stahl experiment, and DNA replication. Structure and types of RNA, RNA as genetic material, transcription, post transcriptional modifications: methylation, capping , splicing & polyadenylation.

Unit: V

Genetic Code: Evidence & Essentiality of Codon, Triplet code, Start and Stop codons. Reading frames, universality of genetic code, Overlapping and split gene. Protein synthesis, in Prokaryotes and eukaryotes and its regulation

Organic Chemistry (BSBT/BSMB-104)

M.M:100 Unit: I (a) Electromeric effects; Inductive effect, mesomeric effect, electromeric effect,Baker-Nathan effect, Hydrogen bonding

(b) Organic intermediates; carbocation, carbanions, free radical, carbenes

(c) Nucleophilic substitution reactions in aliphatic compoundsb (SN1 and SN2), Elimination reactions (E1 and E2)

Unit: II Alkanes, Alkenes , Alkynes and alkyl halides:

Structure, classification, nomenclature synthesis and important reactions of each of the above

Unit: III Alcohols

Structure & Nomenclature, Physical properties, reaction of alcohols: reaction with hydrogen halide, phosphorous trihalide and thionyl chloride, alkyl halide & alkyl phosphates, dehydration, synthesis of ethers from alcohols, Williamson synthesis, oxidation of alcohols.

Unit: IV Chemistry of carbonyl compounds

Structure, Nomenclature and physical properties, Nucleophilic addition to carbon- oxygen double bond, reaction of aldehydes and Ketones, Oxidation, Bayer villager oxidation, reduction to alcohols & hydrocarbons: Clemmensen reduction & Wollf Kishner reduction, addition of water & alcohols (hydrates, hemiacetal, hemiketal ,thioacetal , and thioketals), addition of derivatives of ammonia (reaction with hydroxylamine, hydrazine, phenylhydrazine and semicarbazide), addition of hydrogen cyanide & addition of halides, Witting reaction, Aldol reaction.

Unit: V Aliphatic Carboxylic acids, their derivatives and aliphatic amines:

Structure, Nomenclature, physical properties and acidity of carboxylic acids reaction of carboxylic acids: Nucleophilic substitution at acyl carbon, reduction of carboxylic acids, decarboxylation of carboxylic acids. Structure, nomenclature, physical properties and basicity of amines, reaction of amines; oxidation, reaction of amines with nitrous acids, Hoffmann and cope elimination, Reaction of amides; hydrolysis, conversion to imides, Hoffmann degradation.

Biochemistry (BSMB/BSBT-105) M.M:100

Unit: I Carbohydrates

Monosaccharide: classification, configuration, conformation and derivatives, common disaccharides, structure and occurrence of storage and structural polysaccharides, glycoprotein: structure and functions.

Unit: II Lipids

Fatty acids, triacylglycerole, glycerophospholipids, sphingolipids: sphingomyelins, cerebrosides, and gangliosides, cholesterols, micelles, bilayers, liposome, lipoprotein structure and function.

Unit: III Amino acids and Protein

Amino acids: structure, nomenclature and general properties, peptide bonds, primary structure of proteins: end group analysis, amino acids compositions, specific peptide cleavage and sequence determination, secondary structure: peptide group, Helical structure: α-helix, & other polypeptides helices, β-pleated sheets and Ramachandran plot, protein stability factors: electrostatic interactions, hydrogen bond & hydrophobic forces, disulphide bond, general idea of tertiary and quaternary structure of proteins.

Unit: IV Vitamins and Enzymes Vitamins of B-group, their coenzyme forms, Recommended Dietary Allowance (RDA), source and biochemical function. Fat soluble vitamins: RDA sources and functions.

Unit V: Metabolism

Outlines of following pathways (including name & structure of intermediates, name of enzymes and regulatory aspects but excluding mechanism of enzymes), Glycolysis, TCA cycle, HMP pathway, Glycogen Synthesis and degradation, Gluconeogenesis.

Biostatistics (BSBT-106)

M.M:100

Unit: I

Introduction to biostatistics: Definition, statistical methods, biological measurement, kinds of biological data, function of statistics and limitation of statistics.

Unit: II

Collection of data, sampling and sampling design, classification and tabulation, types of representation [graphic –bar diagram, pie diagram and curves] and basic concept of calculus.

Unit: III

Measures of central tendency, Mean, Median, Mode, Geometric Mean. Measure of dispersion, variability and changes. Deviations- quartile deviation, mean deviation, standard deviation, Standard Error of Mean, coefficient of variations

Unit: IV

Test of significance, Chi-square test, t-test, f-test and ANOVA with numerical

Unit: V

Probability: Random experiment and associated sample space, events definition of probability, algebra of events, addition and multiplication theorems on probability (without proof). Binomial, poisson and normal distributions analysis.

Practical (BSBT/BSMB-107 P)

Bioscience I

Time: 3 hrs. M. M: 75

Q.1 (a) Preparation of temporary mounts to demonstrate stages of mitosis. (10)

(b) Study of permanent slides of different stages of mitosis & meiosis. (15)

Q.2 (a) Section cutting and slides preparation: (20)

(i) Bryophytes: Riccia, Marchantia and Anthoceros

(ii) Pteridophyta: Lycopodium, Selaginella, Equisetum and Pteridium

Q. 3 Spotting (20)

Algae: Nostoc, Chlamydomonas, Volvox, Oedogonium, Chara, Vaucheria,

Polysiphonia and Laminaria

Fungi: Albugo, Penicillium, Puccinia, Alternaria and Agaricus

Bryophyta: Riccia, Marchantia and Anthoceros

Pteridophyta: Lycopodium, Selaginella and Equisetum and Pteridium

.

Q.4 Viva (5)

Q.5.Records (5)


Bioscience II


Q. 1 Dissection: following animals: Prawn, Pila, Unio (20)

Q. 2 Permanent Mounting: Obelia colony (10)

Q.3 Permanent mounting of Meiosis by Grasshopper’s testes or Cheromonas (15)

Q.4 Spotting: (20)

Phylum Protozoa: Euglena,

Phylum: Porifera: Leucosolenia (w.m), Hydra (w.m), Obelia colony, Obelia medusea,

Phylum: Porifera: Euspongia, Spongilla, Hyalonema, Euplectella,

Phylum: Coelenterata: Physalia, Pennatula, Aurelia, Adamsia,

Phylum: Aschelminthes: Ascaris lumbricoides (male and female)

Phylum: Anniledea: Neries, Heteroneries, Chaetopterus, Hirudinaria

Phylum: Mollusca: Pila¸ Mytilus, Octopus, Sepia, Loligo

Phylum: Echinodermeta: Asterias, Holothuria.

Q. 5 Viva (5)

Q. 6 Records. (5)

Q. 6 Records. (5)


Biochemistry & Chemistry


(Part A)

Q.1 Identification of carbohydrate by various methods (15)

(a) Fehling’s solution test

(b) Benedict’s solution test

(c) Barfoed’s solution test

(d) Molish’s solution test

Q.2 Identification of amino acids by various methods (15)

(a) Ninhydrin reaction test

(b) Xanthoproteic test

(c) Biuret test

Q.3 Viva-Voice (5)

Q. 4 Records (5)

(Part B)

Q. 1 Detection of elements by Lassaigne’s test: N, S, Cl, Br, I (25)

OR

Detection of functional group of organic compounds

Q.2 Viva (5)

Q.3 Records (5)

Practical (BSBT -110 P)

Biostatistics


Q.1 Measure the central tendency [Mean, Median and Mode] (20)

OR

Measure the dispersion [Quartile deviation, Range, Mean deviation,

Standard deviation Standard error of Mean and Variance]

Q.2 Basic concept of calculus, test of significance: Chi-square test, t-test, f-test (20)

and ANOVA

Q.3 Graphical representation of data [Histogram, Frequency curve, frequency

Polygon and cumulative frequency] (15)

Q.4 Fitting of Binomial distribution and Basic probability theory (10)

Q.5 Viva (5)

Q.6 Records (5)

Proposed Syllabus for B.Sc Part- II (Biotechnology)

Biomolecules (BSBT/BSMB-201)

Unit: I M.M: 100

Introduction of biomolecules: chemical composition, asymmetric biomolecules: stereospecificty, conformation and configuration. Prebiotic evolution: biochemical and chemical. Water, properties and its effect on dissolved biomolecules.

Unit: II

Carbohydrate metabolism: Structural pathway of Glycolysis (including enzymes involvement kinds of reaction), muscle Glycolysis and alcoholic fermentation, Electron transport system and oxidative phosphorylation.

Unit: III

Lipid metabolism: Biosynthesis of long- chain fatty acids, its regulation and β- oxidation of fatty acids, biosynthesis of triacylglycerole, Biosynthesis of cholesterol.

Unit: IV

Metabolism of protein and amino acids: Biosynthesis of amino acids, oxidation of amino acids, de-carboxylation, transamination and deamination,

Unit: V

Hormone metabolism: General introduction of Animal hormone and its type, functions and deficiency diseases (Excluding endocrine system)

Biophysics and Biophysical Chemistry (BSBT/BSMB-202)

Unit I: M.M:100

Energies of a living body: Sources of heat limit to temperature, heat dissipation and conservation, primary event in photosynthesis, stragies of light reception in microbes, plants and animals, osmotic behavior of living cell, osmosis, tonicity and turgor pressure .

Unit II:

Lamberts-Beer law, Spectrophotometer and colorimeter, general spectroscopy, introduction of electromagnetic spectrum, working principle of IR, UV, VIS and Raman spectroscopy and their application for the characterization of molecules, Introduction and classification of X-rays, X-ray technique to identify the crystal structure of chemical molecules, Basic principle and application of NMR

Unit III: Acid bases and buffer

Introduction (Biological relevance of pH, buffering in living organism, effect of pH on protoplasmic components), concept of acid and bases, the ionization of water, definition of pH, pH scale, Arrhenius, strength of acid and bases, Ionization of monoprotic acid and diproticin in water, Relation between initial acid concentration, pka and pH, Henderson- Hasselbalch equation, dependence of ionization of pH solution uses HOH equation, titration, Salt hydrolysis, Buffer mixture, buffering capacity, pH indictor.

Unit IV: Laws of thermodynamics

General ideas of first law of thermodynamic, enthalpy of systems. Second law, concept of Entropy, Entropy changes in reversible and irreversible processes, Entropy as function of temperature, volume and pressure, Helmholtz and Gibbs free energy functions. Third law of thermodynamics, variation of entropy with temperature, determination of absolute entropies of liquids and gases, application of third law of thermodynamics

Unit V:

Recent development in drugs; General description of new drugs, introduced their chemical nature and biochemistry & application.

Molecular Biology & Animal cell Culture (BSBT/BSMB-203)

M.M:100

(Part A)

Molecular Biology

Unit I:

Post translational modification in brief, and protein targeting to organelles: protein targeting in bacteria, in eukaryotic cells, Insertions elements, transposons,

Unit II:

DNA repair and recombination. DNA sequencing. DNA microarray in brief and Protein analysis.

Unit III:

Cell cycle control and its regulation. Cancer: Molecular biology of cancer, its types. Programmed cell death (Apoptosis)

(Part B)

Animal cell Culture

Unit IV:

History of development of cell culture, the natural surroundings of animal cells, metabolic capabilities of animal cells, simulating natural condition for growing cells, Importance of growth factors of serum.

Unit V:

Primary cultures, anchorage dependence of growth, Non anchorage dependent cells, Secondary cultures, , Continuous cell lines, commonly used animal cell lines – their origin and characteristics. Growth kinetics of cells in culture. Applications of animal cell culture for studies on gene expression.

Genetics (BSBT -204)

Unit: I M.M:100

Mendalian laws of inheritance, Monohybrid cross and the law of segregation, Dihybrid cross and the law of independent assortment, chromosome theory of inheritance, test and back cross Multiple allele

Unit: II

Sex determination in man and Drosophila: Linkage and crossing over, Karyotype, mapping of gene, sex linked inheritance.

Unit: III

Chromosomes: structural organization of chromatids, centromeres, Telomere, Chromatin, Nucleosomes organization, Euchromatin and heterochromatin; special chromosomes (Polytene and Lambrush chromosomes),

Unit: IV

Banding pattern in human chromosomes. Structural and numerical aberration involving chromosomes: hereditary defects- Kleinfelter, Turner, Cri-du chat and Down syndrome, Mutation- and its types,induction of mutation ( chemical and physical mutagens).

Unit: V

Population genetics: Hardy-Weinberg equilibrium, gene and genotypic frequencies,

r-DNA Technology & Genomics (BSBT-205)

M.M:100

(Part A)

r-DNA Technology

Unit: I

Introduction to r-DNA technology, Restriction enzymes, Adaptors and linkers, Joining of DNA molecule.

Unit: II

Isolation and purification of DNA from bacterial, plant and animal cells, Introduction of DNA into living cells, cloning vectors for E.coli, Plasmids, Bacteriophage, Phagemids, Cosmids. General idea of PCR technology

(Part B)

Genomics

Unit: III

Genomics: general idea of Genome, genomics sequences (including example)

Unit: IV

Genome organization: general idea to prokaryotic and eukaryotic, mapping of prokaryotic genome, General idea of human genome project, genomic & cDNA Libraries.

Unit: V

Proteomics: General idea of proteomics, role of proteomics, production of useful protein ( with special reference to Single cell protein), protein folding and denaturation (chaperones).

ENZYMOLOGY & PROTEIN ENGINEERING (BSBT/BSMB -206)

(Part A)

Enzymology

UNIT-I

Enzyme: Definition, classification, zymogens and their activation; Enzyme substrate complex: concept of E-S complex, binding sites, active sites, specificity, energy of activation, kinetics of enzyme activity. Michaelis-Menten equation and its derivation, Different plots for the determination of km and Vmax and their physiological significance; Factors affecting initial rate: Temperature, pH, substrate concentration, enzyme concentration, time, metal ions, modulators etc.; Isolation, crystallization and purification of enzymes, test of homogeneity of enzyme preparation, methods of enzyme assay.

UNIT-II

Two substrate reactions (Random, ordered and ping-pong mechanism) enzyme inhibition, types of inhibition, determination of Ki, suicide inhibitor; Enzyme regulation: Product inhibition, feed back control, covalent modification, Negative co-operativity and half site reactivity, protein-ligand binding, Hill and Scatchard plots, kinetics of allosteric enzymes.

UNIT-III

Isoenzymes: multiple forms of enzymes with reference to lactate dehydrogenase and creatinine phosphokinase; Multienzyme complexes; Ribozymes; Biotechnological and pharmaceutical applications of enzymes

(Part B)

Protein Engineering

UNIT-IV

Technology: methods for large scale production of enzymes; Immobilized enzymes and their comparison with soluble enzymes, methods for immobilization of enzymes, Immobilized Enzyme reactors, Applications of immobilized and soluble enzyme in health and industry; Application to fundamental studies of biochemistry: Artificial enzyme, Enzyme electrodes.

UNIT-V

Thermal stability and catalytic efficiency of enzyme; Site directed mutagenesis and enzyme engineering- selected examples; Delivery system for protein pharmaceuticals, structure functions relationship in enzymes; Structural motifs and enzyme evolution; Methods for protein sequencing; Methods for analysis of secondary and tertiary structures of enzymes; Protein folding in vitro and in vivo.


Biomolecules

Time: 3 hrs. M.M: 75

Q.1 Estimation of protein

(a) Lowry’s method (20)

Q.2 Estimation of carbohydrates

(a) Duboi’s method (OR) Anthrone test (20)

Q.3 Estimation of fats (20) (a) Saponification value (OR) Iodine value

Q.4 Viva (10) Q.5 Records (5)


Biophysical chemistry


Q. 1 Chromatography separation of inorganic ion, sugar and amino acids. (15)

(i) Paper chromatography

(a) Inorganic ion

(b) Sugars

(c) Amino acids

Q.2 TLC (Thin layer chromatography of organic compound) (20)

Q.3 Organic preparation

(A) Drugs (15)

(b) Osazon formation (15)

Q.4 Viva (5)

Q5 Records (5)

Practical (BSBT/BSMB – 209P)

Enzymology & Protein Engineering

Time: 3 hrs. M.M. 75

Q.1. Preparation of Buffers and their testing. (20)

Q.2. Protein Gel Electrophoresis. (15)

Q.3. Enzyme isolation and activity assay from Cuscuta. (15)

Q.4. Immuno (Western) Blotting of Enzyme-Proteins

OR

Precipitation Methods (Ammonium Suphate; Acetone; TCA) (15)

Q.5. Viva- Voce (5) Q.6. Record (5)

Practical (BSBT-210 P)

Biotechnology

Time: 3 hrs. Max. Marks: 75

Q.1 SDS PAGE/ Enzymes isolation: Cuscuta (20)

Q. 2 Serial dilution from soil, air, water (15)

Q.3 Sterilization technique: Autoclaving and Hot air oven, physical and chemicals

method (15)

Q. 4 Technique of inoculation and media preparation: NA, NB, SDA, SDB (15)

Q. 5 Viva (5)

Q. 6 Records (5)

Proposed Syllabus for B.Sc Part- III (Biotechnology)

Animal Biotechnology &Animal Physiology (BSBT-301)

M.M:100

(Part A)

Animal Biotechnology

Unit: I

General principle of animal biotechnology, genetic manipulation: Gene Knock out technology. Marker gene, genetically modified animals: Nude mice, transgenic mice, knock out mice, SCID mice.

Unit: II

Principle of cell culture production of monoclonal antibodies, transplanting cultured cells. General use of transgenic animal and plants in medicine.

(Part-B)

Animal physiology

Unit: III

General and cellular basis of Animal Physiology, Body fluid compartments, transport across cell membrane, homeostasis, intracellular digestion: digestive enzyme, coordination of digestion.

Unit: IV

Muscle and movement: Structure of skeletal, cardiac and smooth muscles, mechanism of muscle contraction., gametogenesis, Female reproductive cycle & hormonal control.

Unit: V

Nervous system and nerve cells, nerve impulses, action potential, structure and functions of sensory organs (olfactory and eyes). Structure and function of hypothalamus, pituitary, thyroid, adrenal and pancreas, neuroendocrine, regulation, secondary messenger concept.

Plant Biotechnology and Plant Physiology (BSBT-302)

M.M:100

(Part A)

Plant Biotechnology

Unit: I

Introduction of plant biotechnology, types of culture: seed culture, embryo culture, callus culture, organ culture and their application. General idea of Micropropagation their applications and advantages.

Unit: II

Haploid and their application. Somaclonal variation and their application. Introduction to protoplast isolation, principle and application, testing of viability of isolating protoplast, various steps in the regeneration of protoplast.

Unit: III

Somatic hybridization; introduction and application of somatic hybridization, various methods for fusing protoplast; chemical, electrical, use of markers for selection of hybrid cell. Introduction to A. tumefaciens and it’s use in genetic transformation

(Part B)

Plant Physiology

Unit: IV

Movement of water in plants in relation to water potential, osmotic potential, pressure potential and metric potential, guttation, transpiration, physiology of stomatal opening and closing. Role of photosynthesis pigments, PS II AND PS I complex and mechanism of photosynthetic electron transport. Photophosphorylation- mechanism of carbon dioxide fixation in C3, C4 and CAM plants, photorespiration.

Unit: V

Plant growth regulators, distribution and their metabolism, mechanism of action of plant growth regulators, role of major plant growth regulators in various plant developmental Processes , importance of growth regulators, phototropism: phototropic signal perception, Gravitropism, growth response, photoperiodism, vernalization. Dormancy: significance, seed dormancy and bud dormancy.

Industrial & Environmental Biotechnology (BSBT-303)

M.M:100

Unit: I

Historical development of industrial biotechnology, industrially important microorganisms, major classes of product and fermentation processes. Fermentors: types of Fermentors and basic functions. Large scale production of acetic acid and citric acid.

Unit: II

Biofertilizer, nitrogen fixing micro-organism. Biopesticides –Bacillus thruingensis, and traditional biocontrol methods, microbiological quality of food and water and biodegradable pathway.

Unit: III

A general concept on pollution, types and ecological impact, Minamata disease, Itai-Itai disease, methaemoglobinemia,

Unit: IV

Bioremediation strategies: Microbial remediation, phytoremediation, phytostablization, phytoextraction, role of chelates in phytoremediation, bioaccumulation and biomagnifications.

Unit: V

Microbial product of pharmaceuticals value- raw materials, organisms and industrial processes involved in the production of penicillin, vitamin B12 and rabies vaccine.

Microbial product of industrial value- raw materials, organism and industrial processes involved in the production of ethanol. Recycling and safe this disposal of industrial wastage through the microbes.

Immunology & Medical Biotechnology (BSBT-304)

M.M:100

Unit: I

Introduction: Terminology- History of immunology- immunohemotology, Rh-incompatibilities Immunity, types of immunity- innate and acquired. Immune systems: General idea of Primary lymphoid organ and Secondary lymphoid organs. Cells of the immune system- T & B cells, receptors, activation and functions.

Unit: II

General idea of humoral and cell mediated immunity, immunity to infectious diseases, autoimmune diseases.

Unit: III

Antigens: types, properties, heptanes-adjuvant, vaccines- types, toxoid, antitoxins, general idea of immunoglobulin- structure their types and properties. Antigens-antibody reaction agglutination, precipitation, immunofluorescence, ELISA, RIA, introduction of hypersensitivity and its types.

Unit: IV

Normal microbial flora of the human body, Host- microbe interaction – virulence factors of microbes. Invasiveness and pathogenicity, Non-specific resistance factors and bacteria causing diseases (name and causative agents).

Unit: V

Mechanism of bacterial and viral pathogenesis, antiviral anti bacterial agent, laboratory diagnosis of viral disease, role of viruses in disease (name and causative agents). General concept of parasitology and antiparasitic agents.

Bioinformatics (BSBT/BSMB-305)

M.M:100

Unit: I Basic concept

Computer and its components; software basics; data vs. information. Firmware, language, compilers, interpreters, MS –Dos, Windows and UNIX, Linux as a UNIX alternative. Searching the web, search engine, web indices, and internet security. Concept of firewell, digital signature, remote login.

Unit: II Introduction to Bioinformatics

What is Bioinformatics? Use of information technology for studying Biosciences, emerging areas of Bioinformatics, future prospects of Bioinformatics.

Unit: III Bioinformatics Basics

Introduction to Genomics, introduction to proteomics, Human Genome project, Biological software, Public data base, Gene Bank,

Unit: IV computational Methods

Gene prediction, sequence alignment and sequence searching, multiple sequence alignment, Phylogenetic analysis, protein sequence analysis, protein structure prediction. Blast and Fasta.

Unit: V Data Base Management systems and Data Mining

Basics of database management system, SQL, Artificial Neural Network technology, Genetic algorithm,

Practical (BSBT-306 P)

Biotechnology


1. Production of acid and enzyme (20)

2. BOD and COD detection. (15)

3. Plant tissue culture (20)

4. Spotting (10)

5. Viva (5)

6. Records (5)


Bioinformatics


1. Sequence Alignment (20)

2. Gene Identification (15)

3. Primer designed (15)

OR

Protein structure determination, secondary and tertiary

4. Uses and application of NCBI and EBI ` (15)

5. Viva (5)

6. Record (5)

B.Sc. Microbiology

M.J.P. ROHILKHAND UNIVERSITY BAREILLY

Examination- Faculty of Science

(A) Eligibility

Bachelor of Science in Microbiology

(B.Sc. in Microbiology)

2. (a) The examination for the degree of Bachelor of science shall consist of three parts:

(4) Part I

(5) Part II

(6) Part III (Final)

(b) A candidate who after passing (i) the intermediate examination (Biology) of the Board of High School and Intermediate Education, Utter Pradesh or of and Indian University incorporated by any law for the time being in force, or (ii) any other examination recognized by the University as equivalent there to, has attended a regular course of study in an affiliated college for one academic year, shall be eligible for appearing at the Part I Examination for the B.Sc. (Microbiology) Degree.

2. (a) A candidate who after passing the Part I B.Sc. Examination of the University, has completed a regular course of the study for one academic year in an affiliated college, shall be eligible for appearing at the Part II B.Sc. (Microbiology) Examination.

A candidate who, after passing Part II B.Sc. (Microbiology) Examination of the University completed a regular course of study for one academic year in an affiliated college shall be eligible for appearing at the Part III B.Sc. (Microbiology) Examination.

(b) A candidate who has passed Part I B.Sc. (Microbiology) Examination from University or any other examination recognized by the University as equivalent to the Part I B.Sc. (Microbiology) examination of the University may also be admitted to the Part II B.Sc. (Microbiology) Examination. Provided that he/she offered for the above mentioned examination of another University a course of an equivalent standard with almost identical

syllabus as required for Part I examination of the University and has attended a regular course of study for one academic year in an affiliated college for the University.

3. Every candidate shall be required to pass in all of the following papers for Part I, II and III of B.Sc. (Microbiology) examinations:

A. Part I: (1) Bioscience I (Plant Diversity) (2) Bioscience II (Animal Diversity) (3) Cell Biology (4) Organic Chemistry (5) Biochemistry (6) General Microbiology

B. Part II: (1) Biomolecules (2) Biophysics & Biophysical Chemistry (3) Molecular Biology & Animal cell culture (4) Microbial Genetics (5) Microbial Physiology (6) Fundamental of Computer & Biostatistics

C. Part III: (1) Medical Microbiology (2) Agriculture, Food & Dairy Microbiology (3) Industrial & Environmental Microbiology (4) Immunology & Virology (5) Information Technology & Bioinformatics (6) Two month training (Entrepreneurship)

4. The examination shall be by means of papers, but candidate shall also be required to undergo a practical examination. Candidates shall be required to pass separately in the Practical Examination.

5. A candidate who desires to enter for an examination under this eligibility criteria must submit his/ her application on a prescribed from so to reach the Registrar not later than 15th November, proceding the date of examination. The application shall be accompanied by an examination fee Rs. 5/- for each for three parts and shall be forwarded by the Principal of the college concerned.

6. There is a uniform pattern of examination in all the papers of for Part I, II and III of B.Sc. (Microbiology) Examination and details are as Follows

7. The minimum pass marks to obtain in each paper shall be 33.

8. The distribution of marks for B.Sc. (Microbiology) Part I, Part II and Part III practical examination throughout will be as follows as per UGC norms.

9. The university rules for Examination/ Evaluation/ Improvement Examination/ Award of grace marks etc. in the above course will be at par with other science graduate courses of the university.

(B) Scheme of Examination

Bachelor of Science (Microbiology)

There shall be three examinations one at the end of each year, the first being the B.Sc. (Microbiology) Part I Examination, the second B.Sc. (Microbiology) Part II examination, and the third B.Sc. (Microbiology) part III final examination.

Candidates will have to pass separately in each of the three examination, but the marks of the three examination will be counted together for a place on the pass list of the part III examination. A candidate for the B.Sc. (Microbiology) examination will be assigned division on the following basis:

First Division 60% of the aggregate marks

Second Division 45%

All the rest in the third Division if the y obtains the minimum pass marks in each paper

Note: Candidates in all papers of B.Sc. (Microbiology) Part I or II and III examinations are allowed to answer question-papers through the medium of English or Hindi (Devanagari script)

Part I B.Sc. I (Microbiology) (A) Theory Papers


1 (BSBT/ BSMB-101)

I Bioscience I (Plant Diversity)

100

2 (BSBT/ BSMB-102)

I Bioscience II (Animal Diversity) 100

3 (BSBT/ BSMB-103)

I Cell Biology 100

4 (BSBT/ BSMB-104) I Organic Chemistry

100

5 (BSBT/ BSMB-105)

I Biochemistry

100

6 (BSMB-106) I General Microbiology

100

Total 600 (B) Practical


Theory Marks Total Marks


I Bioscience I

25 75 100


I Bioscience II 25 75 100


I Biochemistry & Chemistry 25 75 100

4 Practical (BSMB-110 P) I Microbiology

25 75 100

Total 400

(Grand Total (Theory + Practical) = 1000

Part II B.Sc. II (Microbiology) (A) Theory Papers


1 (BSBT/BSMB-201)

II Biomolecules

100

2 (BSBT/BSMB-202)

II Biophysics & Biophysical Chemistry 100

3 (BSBT/BSMB-203)

II Molecular Biology & Animal Cell Culture 100

4 (BSMB-204) II Microbial Genetics & Biostatistics 100

5 (BSMB-205)

II Microbial Physiology

100

6 (BSMB- 206) II Enzymology and Protein Engineering 100 (B) Practical


Theory Marks

Total Marks


II Biomolecules

25 75 100


II Biophysics & Biophysical Chemistry

25 75 100

3 Practical (BSMB-209 P)

II Enzymology and Protein Engineering & Biostatistics

25 75 100

4 Practical (BSMB-210 P) II Microbiolog y& Biostatistics

25 75 100

Total 400


Part III B.Sc. III (Microbiology) (A) Theory Papers

S. No. Papers Code Year Title of the Papers Total Marks 1

(BSMB-301) III

Medical Microbiology 100

2 (BSMB-302)

III Agriculture, Food & Dairy Microbiology 100

3 (BSMB-303)

III Industrial & Environmental Microbiology 100

4 (BSMB-304) III Immunology & Virology 100

5 (BSBT/BSMB-305)

III Bioinformatics

100

Total 500 (B) Practical


Theory Marks

Total Marks

1 Practical (BSMB-306 P)

III Microbiology

25 75 100

2 Practical (/BSBTBSMB-307 P)

III Bioinformatics 25 75 100

3 Entrepreneurship

III Two Months Training 75 225 300

Total 500


Proposed Syllabus for B.Sc. Part I (Microbiology)

Part I B.Sc. I (Microbiology)

(A)Theory papers

Common theory papers

S. No.

Papers Code Title of the Papers

1 (BSBT/ BSMB-101) Bioscience I (Plant Diversity) 2 (BSBT/ BSMB-102) Bioscience II (Animal Diversity)

3 (BSBT/ BSMB-103) Cell Biology

4 (BSBT/ BSMB-104) Organic Chemistry 5 (BSBT/ BSMB-105) Biochemistry

Paper for B.Sc. Microbiology only

6 (BSMB-106) General Microbiology

(B)Practical

Common Practicals

S. No.


1 Practical (BSBT/BSMB-107 P) Bioscience I 2 Practical (BSBT/BSMB-108 P) Bioscience II

3 Practical (BSBT/BSMB-109 P) Biochemistry & Chemistry

Practicals for B.Sc. Microbiology only

4 Practical (BSMB-110 P) Microbiology

General Microbiology (BSMB-106)

M.M:100

Unit I:

Introduction- definition, scope, and history of microbiology. Difference between the prokaryotic and eukaryotic microorganism. Classification of microorganism- general principle of nomenclature, whittaker’s five kingdom concept, Classification and characterization of bacteria according to Bergy’s manual systematics bacteriology (9th edition).

Unit II:

Microbiology: principle and applications of simple, compound, bright field, dark field, phase contrast, fluorescent and electron microscopy, Sterilization principle and method physical (moist heat and dry heat, filtration, pasteurization, tyndallization, radiations) and chemical (alcohols, aldehydes, phenol, halogens and hypochlorite).

Unit III:

General characterization and nature of Archaebacteria, Eubacteria, Rickettsia, Chlamydias, Spirochaetes, Actinomycetes, Viruses including phages (Tphage, Lambda), Mycoplasmas, Principle of staining’s nature of dyes, types of staining – simple, differential, negative and spore staining.

Unit IV:

Microbial cell; ultrastructure of bacteria, sub cellular structure and cell envelope – slime, capsule, cell wall, pili, flagella, cell inclusion, biosynthesis of bacteria cell wall and its composition.

Unit V:

Media: types of simple media, enriched and transport media with specific example for each type, Culture technique: Spread, Streak and pour plate method. Methods of maintenance and preservation of cultures.

Practical (BSMB-110 P)

Microbiology

Time: 3 hrs. Max. Marks: 75

Q. 1 Isolation of microorganism by air, water and soil. (20)

Q. 2 Isolation techniques (10)

(i) Streaking methods

(ii) Pour plate methods.

Q. 3 (a) Preparation of culture media (15)

(b) Sterilization methods – autoclaving and Hot air oven

(c) Staining techniques: Gram’s staining

(d) Techniques of inoculation.

Q. 4 Spotting. (20)

Q. 5 Viva-voice (5)

Q. 6 Records (5)

Proposed Syllabus for B.Sc. Part II (Microbiology)

Part II B.Sc. II (Microbiology)

(A) Theory Papers

Common theory papers

S. No.


1 (BSBT/BSMB-201) Biomolecules 2 (BSBT/BSMB-202) Biophysics & Biophysical Chemistry

3 (BSBT/BSMB-203) Molecular Biology & Animal Cell Culture

6 (BSMB- 206) Enzymology and Protein Engineering

Paper for B.Sc. Microbiology only

4 (BSMB-204) Microbial Genetics & Biostatistics

5 (BSMB-205)

Microbial Physiology

(B)Practical

Common Practicals

S. No.


1 Practical (BSBT/BSMB-207 P) Biomolecules 2 Practical (BSBT/BSMB-208 P) Biophysics & Biophysical Chemistry

3 Practical (BSBT/BSMB-209 P) Enzymology and Protein Engineering & Biostatistics

Practicals for B.Sc. Microbiology only

4 Practical (BSMB-210 P) Microbiolog y& Biostatistics

Microbial Genetics and Biostatistics (BSMB 204)

M.M:100

Unit: I General idea of Mutagenesis- mutation, mutants, phenotypic, mutants- genotypic mutants-IS elements-transposons, repair mechanism, Carcinogenicity testing.

Unit: II Concept of Gene –Lac operon, tryptophan operon, attenuation control, promoters, repressor, gene expression and its regulation

Gene transfer mechanism: conjugation, transformation, transduction.

Unit: III

Organization and function of genetic material of bacterial and viral, Brief account of plasmid-structure- types, Replication of DNA- rolling circle model-theta model.

Unit V:

Definition, statistical methods, biological measurement, kinds of biological data, function of statistics and limitation of statistics. Collection of data, sampling and sampling design, classification and tabulation, types of representation, Graphic, Bar diagram, Pie diagram and curves.

Unit V:

Measures of central tendency, mean, median, mode, geometric mean. Measure of dispersion, variability and changes. Deviations- Quartile Deviation, Mean Deviation, Standard Deviation, Standard Error Mean and Coefficient of variations. Test of significance, Chi-square test, t-test, f-test and ANOVA with numerical

Microbial Physiology (BSMB 205)

M.M:100

Unit: I

Nutrition and growth and microorganism: nutritional types of microorganisms, nutrition requirements factors influencing the growth of microorganism – temperature, pH, Osmotic pressure, moisture, radiation and different chemicals, physiology of growth- significance of various phages of growth. Growth measurement- batch, continuous and synchronous.

Unit: II

Metabolism of proteins- metabolic pathways of nitrogen utilization, urea cycle, synthesis of amino acids, peptides, proteins, pentose pathways,

Unit: III

Bacterial enzymes; Classification, Properties, Co-enzymes, Co-factor, Isozymes, Mechanism of Regulation of enzymes, regulation of enzymes activities, regulation of enzymes synthesis.

Unit: IV

Anaerobic-Respiration and fermentation, Reproductive physiology of microorganisms in general.

Unit: V

Metabolism of carbohydrate: Anabolism – photosynthesis (oxygenic and anoxygenic synthesis of carbohydrate), Catabolism of glucose: EMP Pathway, Pentose Phosphate Pathway.

Practical (BSMB- 210 P)

Microbiology

Time: 3 hrs. M.M: 75

Section A

1. Biochemical test of bacteria: IMVIC and Sugar fermentation (15)

2. Antibiotic sensitivity test: Disc diffusion methods. (10)

3. Water holding capacity of soil. (10)

Section B

4. Measure the central tendency [Mean, Median and Mode] (10)

OR

Measure dispersion [Quartile deviation, Range, Mean deviation

Standard deviation, Standard error of Mean and Variance]

5. Graphical representation of data [Histogram, Frequency curve, frequency (10)

Polygon and Cumulative frequency]

6. Spotting (10)

7. Records (5)

8. Viva-Voice (5)

Proposed Syllabus for B.Sc. Part III (Microbiology)

Part III B.Sc. III (Microbiology)

(A) Theory Papers

Common papers

5 (BSBT/BSMB-305)

Bioinformatics

Papers only for B.Sc. Microbiology

S. No.


1 (BSMB-301) Medical Microbiology 2 (BSMB-302) Agriculture, Food & Dairy Microbiology

3 (BSMB-303) Industrial & Environmental Microbiology

4 (BSMB-304) Immunology & Virology

(B) Practical

Common practicals

2 Practical (/BSBTBSMB-

307 P)

Bioinformatics

3 Entrepreneurship Two Months Training

Practicals only for B.Sc. Microbiology

1 Practical (BSMB-306 P) Microbiology

Medical Microbiology (BSMB-301)

M.M:100

Unit I:

Normal microbial flora of the human body, Host- microbe interaction – virulence factors of microbes. Invasiveness and pathogenicity, Non-specific resistance factors.

Unit II:

Mechanism of bacterial and viral pathogenesis, antiviral and antibacterial agent, laboratory diagnosis of viral disease, general concept of parasitology and antiparasitic agents.

Unit III:

Clinical symptoms: Epidemiology pathogenesis, laboratory diagnosis, prevention and treatment of the following bacterial infection: Tuberculosis, Leprosy, Typhoid, Cholera, Bacillary dysentery, anaerobic wound infection- tetanus and Gas gangrene.

Unit IV:

Clinical symptoms: Epidemiology pathogenesis, laboratory diagnosis, prevention and treatment -of the following viral infection (a) Respiratory infection: Common cold, Influenza, Dengue, and Rabies (b) Liver diseases: Hepatitis A & B (d) Immunodeficiency disease: AIDS

Unit V:

Clinical symptoms: Epidemiology pathogenesis, laboratory diagnosis, prevention and treatment of the following protozoan infection (a) Fungal- superficial, subcutaneous and systemic mycoses, (b) Protozoan: Amoebiasis, Malaria, Leishmaniasis, (c) Helminthes: Filariasis.

Agriculture, Food & Dairy Microbiology (BSMB-302)

M.M. 100

Unit I:

Classification of soil, microflora of various soil types (bacteria, fungi, algae and nematodes in relevance to soil types).

Unit II:

Bio-geological cycles: Carbon, Nitrogen, phosphorous and Sulphur. Biofetilizers, Symbiotic and Non symbiotic nitrogen fixation (Rhizobium & Azotobacter)

Unit III:

Introduction: Importance of food and dairy microbiology – Types of microorganisms in food – Source of contamination (primary source) – Factors influencing microbial growth in food (extrinsic and intrinsic).

Unit IV:

Food poisoning: food born infection Bacterial: staphylococcal, Bacillus, Chlostridium, Escherichia coli

Unit V:

Food preservation: principle of food preservation- methods of preservation. (a) Physical: radiation, drying, heat processing, chilling and freezing, high pressure and modification of atmosphere. (b) Chemical: Sodium benzoate. Introduction of food sanitation.

Industrial & Environmental Microbiology (BSMB-303)

M.M:100

Unit: I

Historical development of industrial microbiology, industrially important microorganisms, Improvement of industrially important microbial strains, types of Fermentors and basic functions, and types of fermentations.

Unit: II

Microbial product of pharmaceuticals value- raw materials, organisms and industrial processes involved in the production of penicillin, vitamin B12 and rabies vaccine.

Unit: III

Concepts of microbial ecology: relationship between microorganisms- Symbiosis, Mutalism, Commensalism, Competition, Ammensalism, Synergism, Parasitism and Predation. Recycling and safe of this disposed industrial wastage through microbes.

Unit: IV

Types of aquatic ecosystems: fresh water- ponds, lakes, eutrophication- food chains, portability of water- microbial assessment of water quality- water purification- brief account of water born disease: Minamata disease, Itai-Itai disease, methaemoglobinemia,

Unit: V

Biodeterioration: deterioration of paper, introduction of Bioremediation, Phytoremediation, Phytoextraction, role of chelates in Phytoremediation, Bioaccumulation and Biomagnifications. Phytoremediation, its disadvantage and mode of prevention.

Immunology and Virology (BSMB-304)

M.M:100

Unit I:

Introduction: Terminology- History of immunology- immunohemotology, Blood group, Blood transfusion - Rh- incompatibilities Immunity, types of immunity- innate and acquired. Immune systems.General idea of -Primary lymphoid organ and Secondary lymphoid organs. cells of the immune system- T & B cells, receptors, activation and functions.

Unit II:

Antigens: types, properties, heptanes-adjuvants, vaccines- types, toxids antitoxins, immunoglobulin- structure, types and properties. Theories of antibody production. Antigens-antibody reaction. Agglutination, precipitation, immunofluorescence, ELISA, RIA. Introduction of Hypersensitivity and its types.

Unit III:

General idea of humoral and cell mediated immunity, immunity to infectious diseases, autoimmune diseases.

Unit IV:

Plant viruses, common plant viral disease: TMV and viroid –double standard DNA virus- Animal viruses: Morphology, pathogenesis and laboratory diagnosis of prions, Rinder pest, Foot and Mouth diseases. Viral vaccines- prevention treatment of viral disease.

Unit V:

Introduction – definition, History of virology. General properties of viruses, structure and replications of viruses- classification of viruses, characterization of viruses, Bacterial viruses – structure of bacteriophages, the lytic life cycle (T- Even coliphages)- lysogenic life cycle ( E. coli, phage Lembda)

Practical (BSMB-306 P)

Microbiology

Time: 3 hrs. M.M: 75 1. Production of antibiotics / Production of organic acid (15)

2. Down stream passing of primary and secondary metabolites (15)

3. MIC: from different antibiotic (15)

4. Spotting (20)

5. Records (5)

6. Viva-Voice (5)

g f College Bsc Biotech and Micro Syllabus

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