FAST RECOMBINANT PROTEIN CHARACTERIZATION WITH … · Protein Analysis. Agilent Accurate-Mass TOF...
Transcript of FAST RECOMBINANT PROTEIN CHARACTERIZATION WITH … · Protein Analysis. Agilent Accurate-Mass TOF...
Accurate Mass TOF LC/MS Systems combined with powerful MassHunter data processing provides answers to your recombinant protein questions.
FAST RECOMBINANT PROTEIN CHARACTERIZATION WITH ACCURATE MASS LC/MS
Robust, Reliable, and Easy to Use
Use TOF LC/MS to determine
• Intact Protein Mass
• Peptide Mapping
• Post-translational Modifications
• Glycan Analysis
For more information visit: www.agilent.com/chem/biopharma
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Rapid Glycoform Analysis by TOF LC/MSAccurate Intact Protein AnalysisAgilent Accurate-Mass TOF and Q-TOF systems simplify your routine protein and mAb analyses. Precise protein/peptide masses are displayed quickly with Agilent’s BioConfirm Software.
Glycan Profiling Made EasyFast Glycan Analysis in just ten minutes with Agilent Q-TOF and the Agilent glycan MS database.
Glycan Profiling
GO-G
N-F N
G1N
GON
GO-F
NM
an6N Man
5N
G1-G
N-F N
G1-G
N NG1
N G1N
G2N
G2N
G2N
Agilent Products are for Research Use Only.Not for use in diagnostic procedures.
Information, descriptions and specifications in thispublication are subject to change without notice.
© Agilent Technologies, Inc. 2013Published in the USA, April 3, 2013
5991-2080EN
See how Agilent Accurate Mass LC/MS technology is ideally suited for the routing
analysis of biotherapeutics: www.agilent.com/chem/biopharma
Find the Right System for Your LabLC/TOF or LC/Q-TOF bring the power of accurate mass to your essential protein characterization tasks.
Ensure ConsistencyComparison of Two Batches of mAbs reveals that batch 2 has a higher percentage and greater variety of sialylated glycan forms.
DescriptionAccurate Mass TOF
Accurate Mass Q-TOF
Intact Masses • •Glycosylation State • •Glycoforms • •Oxidation Changes • •Amino Acids Substitutions • •Peptide Mapping •DeNovo (MS/MS) Peptide Sequencing •DeNovo (MS/MS) Glycan Sequencing •
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10%
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30%
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Relat
ive P
erce
nt
Antibody Batch Comparability
G1 G0 G2
G0-fu
c
G1+s
ilalic
acid
NGN
A
G2+s
ilalic
acid
NGN
A
G1-fu
c
Man
5
G1-fu
c-Gl
cNac
+sia
lic a
cid N
GNA
G1-fu
c +sia
lic a
cid N
GNA
G2-fu
c
Man
8
Batch 1Batch 2
Walk-Up MS-TOF Analysis
Simplified set-up with pre-defined methods for routine characterization.
• Load samples, start method, walk-away
• No optimization needed
• Easy Tools for System Set-Up
• Results emailed to your office