Expression of Nuclear Receptors during Adipogenesis of 3T3-L1 Cells COUP-TFI COUP-TFII PPAR VDR...
Transcript of Expression of Nuclear Receptors during Adipogenesis of 3T3-L1 Cells COUP-TFI COUP-TFII PPAR VDR...
Expression of Nuclear Receptors during Adipogenesis of 3T3-L1 Cells
• COUP-TFI• COUP-TFII• PPAR• VDR• GCNF• ROR• HNF4
• LXR• LXR• RXR• Nur77• REV-ERB• REV-ERB
VDR and COUP-TFII Expression during Adipogenesis of 3T3-L1 Cells
0h 4h 8h 18h 1D 2D 3D 4D 5D 6D 7D 8D
VDR
COUP-TFII
0 6 12 18 24h 2d 3 4 5 6 7 8d
Induction Medium Differentiation Medium
mRNA
Fu et al., Mol. Endo. 19: 2437 (2005)
COUP-TFIIVDR
LXR α
0h 4h 8h 18h 1D 2D 3D 4D 5D 6D 7D 8D
0 6 12 18 24h 2d 3 4 5 6 7 8d
Induction Medium Differentiation Medium
NR Expression during Adipogenesis of 3T3-L1 Cells (2)
Fu et al., Mol. Endo. 19: 2437 (2005)
Nur77
PPAR γ
NUR77LXR PPAR
0h 4h 8h 18h 1D 2D 3D 4D 5D 6D 7D 8D
Actin
COUP-TFI
0 6 12 18 24h 2d 3 4 5 6 7 8d
Induction Medium Differentiation Medium
NR Expression during Adipogenesis of 3T3-L1 Cells
GCNF
Fu et al., Mol. Endo. 19: 2437 (2005)
RXR α
Other Nuclear Receptors
0h 4h 8h 18h 1D 2D 3D 4D 5D 6D 7D 8D
0 2 4 8 18h 1 2 3 4 5 6 7 83T3-L1 cells
Inducing Medium Djffer.Medium
REV-ERB α
REV-ERB β
ROR α
HNF4α
LXR β