Enzyme KJH

8/18/2019 Enzyme KJH

1/23

ENZYMEENZYME

dr. JANGKI HASHUMAL, Sp.BKdr. JANGKI HASHUMAL, Sp.BKDEPARTEMENT OF BIOCHEMISTRYDEPARTEMENT OF BIOCHEMISTRY

FACULTY OF MEDICINEFACULTY OF MEDICINE

HASANUDDIN UNIVERSITYHASANUDDIN UNIVERSITY


2/23

DEFINISIDEFINISINOMENKLATUR DAN KLASIFIKASINOMENKLATUR DAN KLASIFIKASI

INTERAKSI ENZIM-SUBSTRATINTERAKSI ENZIM-SUBSTRAT

KOFAKTOR DAN KOENZIMKOFAKTOR DAN KOENZIMPENGARUH pH DAN SUHUPENGARUH pH DAN SUHU

CONTOH ENZIM SPESIFIKCONTOH ENZIM SPESIFIK


3/23

DEFINISIDEFINISI

Enzim adaa! "i#$a%ai&a%#' Enzim adaa! "i#$a%ai&a%#'

Bi##(i)aBi##(i)a * &i&%+m !id,p* &i&%+m !id,p

S,"&%an&i an( m+nin($a%$an $+)+pa%anS,"&%an&i an( m+nin($a%$an $+)+pa%an'+a$&i $imia %anpa m+n(,"a! !a&i'+a$&i $imia %anpa m+n(,"a! !a&i


4/23

Bi#$a%ai&a%#' Bi#$a%ai&a%#'

P'#%+in d+n(an m#+$, "+&a' P'#%+in d+n(an m#+$, "+&a'

P+'mi% '+a)%i#n& %# (# a% )#ndi%i#n& %!a%P+'mi% '+a)%i#n& %# (# a% )#ndi%i#n& %!a%%!+ "#d )an %#+'a%+%!+ "#d )an %#+'a%+

Can p'#)+&& mii#n& #. m#+),+& +/+'Can p'#)+&& mii#n& #. m#+),+& +/+'&+)#nd&+)#nd

A'+ /+' &p+)i.i) A'+ /+' &p+)i.i) * '+a)% 0i%! #n+ #' #n* '+a)% 0i%! #n+ #' #n

a .+0 %p+& #. m#+),+& 1a .+0 %p+& #. m#+),+& 1substratesubstrate22S,"&%'a%+ i& a &,"&%an)+ %!a% i& a)%+d #nS,"&%'a%+ i& a &,"&%an)+ %!a% i& a)%+d #n" an +nzm+" an +nzm+


5/23

NOMENCLATURE ANDNOMENCLATURE AND

CLASSIFICATIONCLASSIFICATION

Enzm+ n#m+n)a%,'+ i& "a&+d #n 3Enzm+ n#m+n)a%,'+ i& "a&+d #n 3

4!a% i% '+a)%& 0i%!4!a% i% '+a)%& 0i%!

H#0 i% '+a)%&H#0 i% '+a)%& Add Add –ase –ase +ndin(+ndin(

E5amp+& 3E5amp+& 3

La)%a&+ 3 +nzm+ %!a% '+a)%& 0i%! a)%#&+La)%a&+ 3 +nzm+ %!a% '+a)%& 0i%! a)%#&+P',/a%+ d+)a'"#5a&+ 3 '+m#/+& )a'"#5P',/a%+ d+)a'"#5a&+ 3 '+m#/+& )a'"#5.'#m p',/a%+.'#m p',/a%+


6/23

Enzm+ ,&,a 0'i%%+n inEnzm+ ,&,a 0'i%%+n in trivial nametrivial name ",% +/+'",% +/+'

+nzm+ !a& an+nzm+ !a& an official nameofficial name .'#m In%+'na%i#na.'#m In%+'na%i#na

Uni#n #. Bi#)!+mi&%' 1Enzm+ N#m+n)a%,'+Uni#n #. Bi#)!+mi&%' 1Enzm+ N#m+n)a%,'+

6778267782

Ba&+d #n %!+ #..i)ia n#m+n)a%,'+ &&%+m9 +a)!

+nzm+ !a& a ., nam+ and an +nzm+)#mmi&&i#n n,m"+' 1E:C2 d+'i/+d in %!+ p#&i%i#n

in %!+ )a&&i.i)a%i#n &)!+m+

E5amp+ 3

H+5#$ina&+ !a& %!+ ., nam+ ATP3 D-!+5#&+ ;-

p!#&p!#%'an&.+'a&+ and %!+ )a&&i.i)a%i#n

n,m"+' E:C 8:


7/23

Ca&&i.i)a%i#n #. +nzm+&Ca&&i.i)a%i#n #. +nzm+&

Ba&+d #n %p+ #. '+a)%i#n9 +nzm+& !a/+Ba&+d #n %p+ #. '+a)%i#n9 +nzm+& !a/+"++n )a&&i.i+d %# &i5 ma=#' )a&&+& a&"++n )a&&i.i+d %# &i5 ma=#' )a&&+& a&

.##0& 3.##0& 3

6:6: O5id#'+d,)%a&+&O5id#'+d,)%a&+& 3 )a%az+ #5ida%i#n-3 )a%az+ #5ida%i#n-'+d,)%i#n '+a)%i#n&'+d,)%i#n '+a)%i#n&

8:8: T'an&.+'a&+&T'an&.+'a&+& 3 )a%az+ %'na&.+' #.3 )a%az+ %'na&.+' #.

.,n)%i#na ('#,p& .'#n #n+ m#+),+ %#.,n)%i#na ('#,p& .'#n #n+ m#+),+ %#an#%!+' an#%!+'

>:>: Hd'#a&+&Hd'#a&+& 3 )a%az+ !d'#%i) )+a/a(+3 )a%az+ !d'#%i) )+a/a(+


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?:?:La&+&La&+&

3 )a%az+ '+m#/a #. a ('#,p3 )a%az+ '+m#/a #. a ('#,p

.'#m #' addi%i#n #. a ('#,p %# a d#,"+.'#m #' addi%i#n #. a ('#,p %# a d#,"+

"#nd9 #' #%!+' )+a/a(+& in/#/in("#nd9 #' #%!+' )+a/a(+& in/#/in(

++)%'#n '+a''an(m+n%++)%'#n '+a''an(m+n%

@:@: Im+'a&+&Im+'a&+& 3 )a%az+d in%'am#+),+'3 )a%az+d in%'am#+),+'

'+a''an(+m+n%'+a''an(+m+n%

;:;: Li(a&+&Li(a&+& 3 )a%az+d '+a)%i#n& in 0!i)!3 )a%az+d '+a)%i#n& in 0!i)!

%0# m#+),+& a'+ =#in+d%0# m#+),+& a'+ =#in+d


9/23


10/23

ENZYME-SUBSTRATEENZYME-SUBSTRATE

INTERACTIONINTERACTION

UNCATALYZED REACTIONUNCATALYZED REACTION

EE

NN GGBB

EE SUBSTRATESUBSTRATE

RR

GGPRODUCTPRODUCT

YY

VELOCITYVELOCITY

E..+)% #. +nzm+ #n a)%i/a%i#nE..+)% #. +nzm+ #n a)%i/a%i#n+n+'( 3+n+'( 3

A '+a)%i#n m,&% (+% #/+' %!+ A '+a)%i#n m,&% (+% #/+' %!+a)%i/a%i#n +n+'( !,'d+a)%i/a%i#n +n+'( !,'d+


11/23

CATALYZED REACTIONCATALYZED REACTION

EE

NNEE

R S,"&%'a%+R S,"&%'a%+

GG

Y P'#d,)%Y P'#d,)%

VELOCITYVELOCITY

Enzm+ )!an(+ !#0Enzm+ )!an(+ !#0a '+a)%i#n 0ia '+a)%i#n 0i

p'#)++dp'#)++d

Enzm+ '+d,)+& %!+Enzm+ '+d,)+& %!+

a)%i/a%i#n +n+'(a)%i/a%i#n +n+'(

And i% ma$+& %!+ And i% ma$+& %!+

'+a)%i#n .a&%+''+a)%i#n .a&%+'


12/23

E..+)% #. &,"&%'a%+ )#n)+n%'a%i#n3E..+)% #. &,"&%'a%+ )#n)+n%'a%i#n3

F#' n#n-)a%az+d '+a)%i#n3F#' n#n-)a%az+d '+a)%i#n3

R+a)%i#n 'a%+ in)'+a&+ 0i%! )#n)+n%'a%i#nR+a)%i#n 'a%+ in)'+a&+ 0i%! )#n)+n%'a%i#n

F#' +nzm+ )a%az+d '+a)%i#n 3F#' +nzm+ )a%az+d '+a)%i#n 3

A in)'+a&+9 ",% #n %# a )+'%ain p#in% A in)'+a&+9 ",% #n %# a )+'%ain p#in%

VVma5ma5-- ma5im,m /+#)i%ma5im,m /+#)i%

A% V A% Vma5ma59 %!+ +nzm+ i& 0#'$in( a& .a&% a& p#&&i"+9 %!+ +nzm+ i& 0#'$in( a& .a&% a& p#&&i"+


13/23

C!a'a)%+'i&%i)& #. +nzm+ a)%i/+ &i%+C!a'a)%+'i&%i)& #. +nzm+ a)%i/+ &i%+

Ca%a%i) &i%+Ca%a%i) &i%+4!+'+ %!+ '+a)%i#n a)%,a #)),'&4!+'+ %!+ '+a)%i#n a)%,a #)),'&

Bindin( &i%+Bindin( &i%+

A'+a %!a% !#d& &,"&%'a%+ in p'#p+' pa)+ A'+a %!a% !#d& &,"&%'a%+ in p'#p+' pa)+Enzm+& ,&+& 0+a$9 n#n-)#/a+n%Enzm+& ,&+& 0+a$9 n#n-)#/a+n%in%+'a)%i#n& %# !#d &,"&%'a%+ in pa)+in%+'a)%i#n& %# !#d &,"&%'a%+ in pa)+

"a&+d #n R ('#,p& #. amin# a)id&"a&+d #n R ('#,p& #. amin# a)id&Si%+& a'+ p#)$+%& #' )+.% #n %!+ +nzm+Si%+& a'+ p#)$+%& #' )+.% #n %!+ +nzm+&,'.a)+&,'.a)+


14/23

S%+p& in an +nzm+ '+a)%i#nS%+p& in an +nzm+ '+a)%i#n

Enzm+ and &,"&%'a%+ )#m"in+ %# .#'m aEnzm+ and &,"&%'a%+ )#m"in+ %# .#'m a)#mp+5)#mp+5

C#mp+5 (#+& %!'#,(! a %'an&i%i#n &%a%+C#mp+5 (#+& %!'#,(! a %'an&i%i#n &%a%+

A )#mp+5 #. A )#mp+5 #.%!+ +nzm+ and%!+ +nzm+ and

%!+ p'#d,)% i&%!+ p'#d,)% i&

p'#d,)+dp'#d,)+d

T!+ +nzm+T!+ +nzm+and p'#d,)%and p'#d,)%

&+pa'a%+&+pa'a%+


15/23

C!a'a)%+'i&%i) #. +nzm+ a)%i/+ &i%+C!a'a)%+'i&%i) #. +nzm+ a)%i/+ &i%+

L#)$ and $+ m#d+L#)$ and $+ m#d+Pi)%,'+ " Emi Fi&!+' 1672Pi)%,'+ " Emi Fi&!+' 1672

T!i& m#d+ a&&,m+d %!a% #n a &,"&%'a%+T!i& m#d+ a&&,m+d %!a% #n a &,"&%'a%+

#. %!+ p'#p+' &!ap+ )#,d .i% 0i%! %!+#. %!+ p'#p+' &!ap+ )#,d .i% 0i%! %!++nzm++nzm+


16/23

Ind,)+d-.i% m#d+Ind,)+d-.i% m#d+

P'#p#&+d " Dani+ K#&!and 167@2P'#p#&+d " Dani+ K#&!and 167@2

T!i& m#d+ a&&,m+& )#n%in,#,& )!an(+& inT!i& m#d+ a&&,m+& )#n%in,#,& )!an(+& in

a)%i/+ &i%+ &%',)%,'+ a& a &,"&%'a%+ "ind&a)%i/+ &i%+ &%',)%,'+ a& a &,"&%'a%+ "ind&

T!i& m#d+ '+)#(niz+& %!a% %!+'+ i& m,)!T!i& m#d+ '+)#(niz+& %!a% %!+'+ i& m,)!

.+5i"ii% in an.+5i"ii% in an

+nzm+& &%',)%,'++nzm+& &%',)%,'+

A))#'din( %# %!i& A))#'din( %# %!i&

m#d+9 an +nzm+m#d+9 an +nzm+i& a"+ %# )#n.#'mi& a"+ %# )#n.#'m

%# a &,"&%'a%+%# a &,"&%'a%+


17/23

COFACTORS AND COENZYMESCOFACTORS AND COENZYMES

A n#np'#%+in )#mp#n+n% #. +nzm+& i& )a+d A n#np'#%+in )#mp#n+n% #. +nzm+& i& )a+d

%!+%!+ )#.a)%#' )#.a)%#' : C#.a)%#'& )an "+ +i%!+' #'(ani) #': C#.a)%#'& )an "+ +i%!+' #'(ani) #'

in#'(ani) )#mp#,nd& :I. %!+ )#.a)%#' i& #'(ani)9in#'(ani) )#mp#,nd& :I. %!+ )#.a)%#' i& #'(ani)9

%!+n i% i& )a+d a%!+n i% i& )a+d a )#+nzm+)#+nzm+: C#+nzm+& a'+: C#+nzm+& a'+

'+a%i/+ &ma m#+),+& )#mpa'+d %# %!+'+a%i/+ &ma m#+),+& )#mpa'+d %# %!+

p'#%+in pa'% #. %!+ +nzm+: Man #. %!+p'#%+in pa'% #. %!+ +nzm+: Man #. %!+

)#+nzm+& a'+ d+'i/+d .'#m /i%amin&: T!+)#+nzm+& a'+ d+'i/+d .'#m /i%amin&: T!+in#'(ani) )#.a)%#'& )#mm#n a'+ m+%a i#n&:in#'(ani) )#.a)%#'& )#mm#n a'+ m+%a i#n&:

1+:(: Ca9 C,9 C#9 F+21+:(: Ca9 C,9 C#9 F+2


18/23

Enzm+& %!a% '+,i'+ a )#.a)%#'9 ",% d#Enzm+& %!a% '+,i'+ a )#.a)%#'9 ",% d#

n#% !a/+ #n+ "#,nd a'+ )a+dn#% !a/+ #n+ "#,nd a'+ )a+dap#+nzm+: An ap#+nzm+ %#(+%!+' 0i%!ap#+nzm+: An ap#+nzm+ %#(+%!+' 0i%!

i%& )#.a)%#'1&2 i& )a+d a !##+nzm+ 1i%& )#.a)%#'1&2 i& )a+d a !##+nzm+ 1 i.e.,i.e.,

%!+ a)%i/+ .#'m2%!+ a)%i/+ .#'m2

COENZYME SUBSTRATE NO PRODUCT

APOENZYME SUBSTRATE NO PRODUCT

APOENZYME COENZYME SUBSTRATE PRODUCT

HOLOENZYME


19/23

Some vi!mi"# $! !re o%e" &o"veredSome vi!mi"# $! !re o%e" &o"vered oo &oe"'(me# )&oe"'(me# )

Vi%aminVi%amin C#+nzm+C#+nzm+ F,n)%i#nF,n)%i#n

B6 1T!iamin+2B6 1T!iamin+2 T!iamin+T!iamin+p'#p!#&p!a%+p'#p!#&p!a%+

D+)a'"#5a%i#nD+)a'"#5a%i#n

B8 1Ri"#.a/in2B8 1Ri"#.a/in2 Fa/in ad+nin+Fa/in ad+nin+din,)+#%id+ 1FAD2din,)+#%id+ 1FAD2 Ca''i+& !d'#(+nCa''i+& !d'#(+n

B> 1Nia)in2B> 1Nia)in2 Ni)#%inamid+Ni)#%inamid+ad+nin+ad+nin+din,)+#%id+ 1NAD2din,)+#%id+ 1NAD2

O5ida%i#n #'O5ida%i#n #'!d'#(+n %'an&.+'!d'#(+n %'an&.+'

F#i) a)idF#i) a)id T+%'a!d'#.#i) a)idT+%'a!d'#.#i) a)id Amin# a)id Amin# a)idm+%a"#i&mm+%a"#i&m

Pan%#%!+ni) a)idPan%#%!+ni) a)id C#+nzm+ AC#+nzm+ A A) ('#,p )a''i+' A) ('#,p )a''i+'


20/23

EFFECT OF pH TEMPERATURE ONEFFECT OF pH TEMPERATURE ON

ENZYMATIC REACTIONSENZYMATIC REACTIONS

E5)++din( n#'ma pHE5)++din( n#'ma pHand n#'ma %+mp+'a%,'+and n#'ma %+mp+'a%,'+'an(+& a0a& '+d,)+&'an(+& a0a& '+d,)+&+nzm+ '+a)%i#n& 'a%+&+nzm+ '+a)%i#n& 'a%+&Op%im,m %+mp+'a%,'+ i&Op%im,m %+mp+'a%,'+ i&,&,a 8@-?,&,a 8@-?CC

F#' pH9 m#&% +nzm+&F#' pH9 m#&% +nzm+&0#'$ n+a' pH


21/23

SPECIFIC ENZYME EJAMPLESPECIFIC ENZYME EJAMPLE

CHYMOTRYPSINCHYMOTRYPSIN

A p'#%+#%i) +nzm+ A p'#%+#%i) +nzm+

Di(+&%i#n #. di+%a' p'#%+in in &ma in%+&%in+Di(+&%i#n #. di+%a' p'#%+in in &ma in%+&%in+

Ini%ia p'#d,)+d in an a)%i/+ .#'m 1zm#(+n2 3Ini%ia p'#d,)+d in an a)%i/+ .#'m 1zm#(+n2 3

)!m#%'p&in#(+n and a)%i/+d " %'ip&in)!m#%'p&in#(+n and a)%i/+d " %'ip&in

C!m#%'ip&in #n 0#'$& #n amin# a)id&C!m#%'ip&in #n 0#'$& #n amin# a)id&

)#n%ainin( an a'#ma%i) 'in( 1p!+naanin9)#n%ainin( an a'#ma%i) 'in( 1p!+naanin9%'#&in+9 %'p%#p!an2%'#&in+9 %'p%#p!an2


22/23

CONCLUSIONCONCLUSION

ENZYME DEFENITIONENZYME DEFENITIONENZYME NOMENCLATURE ANDENZYME NOMENCLATURE AND

CLASSIFICATIONCLASSIFICATION

ENZYME-SUBSTRATE INTERACTIONENZYME-SUBSTRATE INTERACTIONCOFACTOR AND COENZYMECOFACTOR AND COENZYME

EFFECT OF pH TEMPERATUREEFFECT OF pH TEMPERATURE


23/23

REFERENCESREFERENCES

B'#0ni+ AC9 K+'n#!an C: Bi#)!+mi&%': AB'#0ni+ AC9 K+'n#!an C: Bi#)!+mi&%': A)#'+ %+5% 0i%! &+.-a&&+&m+n%: C!,')!i)#'+ %+5% 0i%! &+.-a&&+&m+n%: C!,')!iLi/in(&%#n+9 6777:Li/in(&%#n+9 6777:

Camp"+ PN9 Smi%! AD: Bi#)!+mi&%'Camp"+ PN9 Smi%! AD: Bi#)!+mi&%'I,&%'a%+d ?I,&%'a%+d ?%!%! +d: C!,')!i Li/in(&%#n+9 8:+d: C!,')!i Li/in(&%#n+9 8:Ma%!+0& CK9 /an H#d+ KE9 A!'+n KG:Ma%!+0& CK9 /an H#d+ KE9 A!'+n KG:Bi#)!+mi&%' >%! +d9 Addin-4+&+Bi#)!+mi&%' >%! +d9 Addin-4+&+P,"i&!in( C#mpan9 8:P,"i&!in( C#mpan9 8:

S0amina%!an R: Hand"##$ #. Cini)aS0amina%!an R: Hand"##$ #. Cini)aBi#)!+mi&%': N+0 D+!i: O5.#'d Uni/+'&i%Bi#)!+mi&%': N+0 D+!i: O5.#'d Uni/+'&i%P'+&&9 8?:P'+&&9 8?:

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