Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis...

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Transcript of Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis...

Page 1: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane
Page 2: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane

Endocytosis

Page 3: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane

Three types of coated vesicles transport proteinsfrom organelle to organelle

Lodish et al, 2000

Page 4: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane

Lilly (1993) Pathophysiology of Heart Disease

A single layer of endothelial cells lines arteries

Page 5: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane

Lilly (1993) Pathophysiology of Heart Disease

Fatty streaks contain macrophage cells with lipid deposits

Page 6: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane

FibrousPlaques

Calcification

Page 7: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane

Lilly (1993) Pathophysiology of Heart Disease

Fibrous plaques damage the endothelial layer

Page 8: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane

Pathology Advances

Ulceration Thrombosis

Page 9: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane

Brown & Goldstein (1986) Science 232:34

Cholesterol is carried mostly aslow density lipoprotein (LDL) particles

Page 10: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane

HMGCoA controls the rate limiting stepin cholesterol synthesis

Zubay, 1998

Page 11: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane

Receptor-mediated endocytosisvia clathrin-coated pits and vesicles

Lodish et al, 2000

Page 12: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane

Brown & Goldstein (1986) Science 232:34

LDL receptor recycling pathway

Page 13: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane

EM image of clathrin-coated pitfrom cytosolic side

Lodish et al, 2000

Page 14: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane

Structure of a clathrin-coated vesicle

Lodish et al, 2000

Page 15: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane

Smith & Pearse. Trends in Cell Biology, 1999

Page 16: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane

movie by Allison Bruce

Assembly of clathrin coated vesicle

Page 17: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane

Assembly and vesiculation of clathrin-coated pits

Lodish et al, 2000

Page 18: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane

Dynamin is a GTPase required for pinching off ofclathrin-coated vesicles

Lodish et al, 2000

Page 19: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane

Components that participate inbudding of coated vesicles

Lodish et al, 2000

Page 20: Endocytosis - California Institute of Technologymbklab/courses/bi113/Lecture 5.pdf · endocytosis (-60 nm) Clathrin- and caveolin-independent endocytosis (-90 nm) P asrna nVnbrane

Journal of Chemical and Pharmaceutical Research · through the fenestrae between discontinuous endothelial cells (30 to 500 nm). The entrance of a particle into cells via endocytosis

Journal of Chemical and Pharmaceutical Research · through the fenestrae between discontinuous endothelial cells (30 to 500 nm). The entrance of a particle into cells via endocytosis

Analysis of endocytosis at eisosomes

Analysis of endocytosis at eisosomes

Endocytosis & Exocytosis Pooja Patel Elizabeth Pemberton.

Endocytosis & Exocytosis Pooja Patel Elizabeth Pemberton.

Active Transport, Endocytosis, and Exocytosis

Active Transport, Endocytosis, and Exocytosis

Endocytosis, Midwest 2011

Endocytosis, Midwest 2011

Passive transport Active transport Exocytosis Endocytosis

Passive transport Active transport Exocytosis Endocytosis

Endocytosis in Notch Signaling Activation · the endocytosis-mediated regulation of signaling have been uncovered: (i) endocytosis can . 332 Molecular Regulation of Endocytosis activate/modulate

Endocytosis in Notch Signaling Activation · the endocytosis-mediated regulation of signaling have been uncovered: (i) endocytosis can . 332 Molecular Regulation of Endocytosis activate/modulate

Endocytosis & Exocytosis By : Matt Sargeant. What is Endocytosis and Exocytosis? Endocytosis is a process by which cells absorb molecules (such as proteins)

Endocytosis & Exocytosis By : Matt Sargeant. What is Endocytosis and Exocytosis? Endocytosis is a process by which cells absorb molecules (such as proteins)

Exocytosis and endocytosis

Exocytosis and endocytosis

Dynamic networks & clathrin-mediated endocytosis

Dynamic networks & clathrin-mediated endocytosis

Clathrin-mediated endocytosis

Clathrin-mediated endocytosis

Exocytosis and endocytosis: from synaptic vesicles to nanodiscs · 2018-01-12 · Endocytosis in vitro Chair: Sandy Schmid 14:00 Regulation of clathrin-mediated endocytosis Sandra

Exocytosis and endocytosis: from synaptic vesicles to nanodiscs · 2018-01-12 · Endocytosis in vitro Chair: Sandy Schmid 14:00 Regulation of clathrin-mediated endocytosis Sandra

M G OLF C URSE SERV ICE C V E R · nm nm nm nm nm nm nm nm nm \ \ \ nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm n nm nm nm nm nm nm nm nm nm nm nm nm

M G OLF C URSE SERV ICE C V E R · nm nm nm nm nm nm nm nm nm \ \ \ nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm n nm nm nm nm nm nm nm nm nm nm nm nm

transport bulk endocytosis

transport bulk endocytosis

Endocytosis*&*Exocytosis**abramsbiology.weebly.com/.../endo_and_exocytosis.pdf · Endocytosis** The*process*by*which*amolecule*gets*into*acell* withoutpassing*through*the*membrane*

Endocytosis*&*Exocytosis**abramsbiology.weebly.com/.../endo_and_exocytosis.pdf · Endocytosis** The*process*by*which*amolecule*gets*into*acell* withoutpassing*through*the*membrane*

geospasial.bnpb.go.idgeospasial.bnpb.go.id/.../PETA-SEBARAN-PENDIDIKAN-DI-SEKITAR-GUNUNG... · nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm

geospasial.bnpb.go.idgeospasial.bnpb.go.id/.../PETA-SEBARAN-PENDIDIKAN-DI-SEKITAR-GUNUNG... · nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm nm

Regulation of EGFR endocytosis by CBL during mitosis · 11 Endocytosis plays important roles in regulating EGFR signaling. We previously found 12 that EGFR endocytosis during mitosis

Regulation of EGFR endocytosis by CBL during mitosis · 11 Endocytosis plays important roles in regulating EGFR signaling. We previously found 12 that EGFR endocytosis during mitosis

Active Transport: Protein Pumps and Endocytosis

Active Transport: Protein Pumps and Endocytosis

Mechanisms of Endocytosis

Mechanisms of Endocytosis

Endocytosis and exocytosis

Endocytosis and exocytosis