DNA Fingerprinting DNA Fingerprinting. At the end of this lesson, I will be able to 0 Explain how...
-
Upload
myrtle-watts -
Category
Documents
-
view
215 -
download
1
Transcript of DNA Fingerprinting DNA Fingerprinting. At the end of this lesson, I will be able to 0 Explain how...
At the end of this lesson, I will be able to
0Explain how DNA Fingerprinting is related to the structure of a chromosome
0Explain how the ability to create a DNA Fingerprint is based on evolutionary change in the human genome
0Analyze a gel of DNA Fingerprints to determine paternity and the guilt/innocence of criminal suspect
Today’s critical vocabulary
0Chromosome0Coding DNA0Non-Coding DNA0Repeating DNA0Homologous pairs0Restriction Enzymes0Gel Electrophoresis
Coding versus non-Coding DNA
Gene 1
Gene 2
Gene 3
Gene 4
Non Coding DNA
Non Coding DNA
Non Coding DNA
Non Coding DNA
Non Coding DNA
A Fingerprint Requires Variation—Where will we find variation in the
chromosome?Discuss with a partner.Come up with a rationale for your answer.
Can you give examples of what happens when there is variation is coding section
of DNA aka a gene?
Gene 1
Gene 2
Gene 3
Gene 4
Non Coding DNA
Non Coding DNA
Non Coding DNA
Non Coding DNA
Non Coding DNA
The variability in the human genome is in the non-coding regions of the chromosome
Since mutations in DNA are TOTALLY RANDOM; occurring equally within coding and noncoding regions;
WHY DO WE SEE SO FEW VARIATIONS WITHIN GENES AND SUCH VARIATION IN THE NON-CODING REGIONS?
The answer is NATURAL SELECTION
0 Individuals who have variations within the coding regions of the DNA that are detrimental are selected AGAINST and do no live to pass that variation on to offspring.
0 Individual who have variations within the non-coding regions have no negative affects. This is an example of a neutral mutation.
0Since this mutation has no effect, these mutations or variations are passed along to offspring.
Let’s ReflectWhat is the primary difference between coding DNA and non-coding DNA?A. Coding DNA is made of all four possible DNA
nucleotides; non-coding DNA contains only three of the possible four nucleotides.
B. Coding DNA is less susceptible to mutation than is non-coding DNA.
C. Non-coding DNA does not contain a sequence that includes instructions for a protein.
D. Coding DNA always stains more darkly than does non-coding DNA.
Let’s ReflectWhy are the DNA sequences of the coding region of DNA maintained generation after generation—even though mutations do occur in these regions?A. The mutations in the coding regions are always
repaired by DNA repair mechanisms.B. Errors or mutations in the coding sections of the
chromosome result in offspring that most likely will not pass the mutation to the next generation.
C. Natural selection corrects any errors that are made in the coding section of a chromosome.
D. Organisms can automatically repair any change in the sequence of the coding region of a chromosome.
Using the Non-coding region to make a DNA Fingerprint
How?1. DNA is sequenced, identifying coding and non-
coding regions.2. Non-coding regions are famous for REPEATING
SEQUENCES OF NUCLEOTIDES.3. One individual may have one nucleotide repeat
(TAA); a second individual may have that sequence repeated 50 times (TAATAATAATAATAA, etc.)
4. This results in different lengths of DNA in the same area of the chromosome
Restriction Enzymes & Gel Electrophoresis
0The section of the chromosome is cut out by the correct restriction enzyme
0The sections of DNA are then run through a gel electrophoresis where DNA fragments of different sizes are separated
0The gel can then be analyzed