Diseases of the pulp:Part 1- Development, Physiology, Histology of Dental Pulp
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Transcript of Diseases of the pulp:Part 1- Development, Physiology, Histology of Dental Pulp
“The pulp is a small tissue with a big issue” - I. B. Bender
DISEASES OF THE DENTAL PULP- Part I
DEEPTHI P.R.Ist YEAR MDSDEPT. OF CONSERVATIVE DENTISTRY AND ENDODONTICS
Introduction Development of pulp - disorders Pulp as a connective tissue - Cellular elements - Fibers - Ground substance - Vasculature - Nerve supply - Systemic factors affecting the pulp Dental pulp stem cells Conclusion
INTRODUCTION Unique tissue Soft tissue : mesenchymal origin Integral part of dentin – dentin pulp complex Rigid encasement: low compliance environment Incompressible: inflammation- increased tissue
pressure External communication: apical foramen & lateral
canals
DEFINITION‘A richly vascularized and innervated specialized connective tissue of ectomesenchymal origin; contained in the central space of a tooth, surrounded by the dentin, with inductive,formative, nutritive, sensory and protective functions’ - Glossary of Endodontic terms
FUNCTIONS PRIMARY: Formative
SECONDARY: tooth sensitivity, defense & hydration, nutrition
Odontoblasts:
Dentinogenesis
Interaction with dental epithelium:
Amelogenesis
DEVELOPMENT Downgrowths from dental lamina: Enamel organ Stages: Bud, Cap & Bell- deepening of
invagination Tissue within the invagination: ‘ Dental Papilla’
DEVELOPMENT 8th week IUL:
beginning of papilla Bell stage: inner layer
of papilla- odontoblasts dentin
Dental pulp: Cephalic neural crest cells
Blood supply Oval/ Circular reticulated plexus in
alveolar bone (Saunders-1966 & Cutright-1970)
Series of blood vessels- dental papilla: future pulpal vessels
Vessels in dental sac basal wall: course to papilla (Tobin 1972)
Pulpal artery: plexus of vessels at pulpodentinal junction
Byers (1980)
Nerve supply Early development: few axons enter
papilla- no peripheral nerve plexuses Eruptive stage: rapid development -
plexus of Raschkow & terminals in odontoblastic layer
Disorders: pulp development
Vitamin D deficiency Down’s syndrome: Jaspers -1981 Dens invaginatus Pulpal dysplasia : Witkop- 1973 Regional odontodysplasia Hypophosphatasia: Houpt et al (1970) Beumer et al (1973) Hereditary hypophosphatemia: Archard &
Witkop (1966) Hypophysectomy
Pulp as a Connective Tissue Cells, ground substance, fibers Cells: a fundamental matrix Site & precursor for the fiber complex Collagen & reticulin End product of the system
Cells of the Pulp Fibroblasts Odontoblasts Defense & other cells
Fibroblasts Basic cell type Baume: mesenchymal cells,
pulpoblasts, or pulpocytes- progressive levels of maturation
Active in collagen synthesis: fibers present on the cell body & processes
Galdames et al,Int. J. Morphol. vol.29 no.1 Temuco mar. 2011
Fibroblasts Synthesize 6
Glycoproteins: fibronectin
Fibronectin with Type III collagen: Reticulin fibers
faint metachromasia, phosphatase & ATP acitivity
Fibroblasts With age: more number & width of
fibers & cells reduce More fibrous pulp: less defensive than
young cellular pulp Responsible for increase in size of
denticles
Odontoblasts Highly differentiated cell in pulp Main function: dentin production Uniformly stained hyperchromatic in
tissue sections Cytoplasm: may or may not be evident
Marion D et al, 1991
Morphologic variations:
A, Pulp horn (pear shaped)B, coronal midpulp (spindle shape)C, coronal midroot level (elongated clubshape)D, mid-third of root (short club shape)E, apical third of root (globules).
Electron Microscope Findings
Large, closely aligned, multilayered sweet potato shaped cells
3 to 4 µm wide & 8 to 10 µm longNucleus:• Ellipsoidal – chromatin & nucleolus• Double membrane covered• Granules attached to outer membrane
Electron Microscope FindingsNucleolus: One to four in number ( Ivanyi 1972) Ring shaped: fully developed- inactive
RNA synthesis Compact: less developed- active RNA
synthesis
Electron Microscope FindingsCytoplasm: Extensive rER & numerous transitional
vesicles (Jesson-1968, Garant et al & Reith -1968, Takuma & Nagai- 1971)
Vesicles: fine fibrillar material Large Golgi apparatus : centre Membrane bound granules: lysosomes Secretory granules- abacus bodies: golgi
complex
Electron Microscope Findings
Mitochondria evenly distributed
Centrioles present : rudimentary cilium
Approx. 50 Ao diameter filaments
200 to 250 Ao diameter microtubules
Electron Microscope Findings Odontoblasts : 6-8 cells deep, palisade
formation along predentin border Organelles: extend to terminal bar
apparatus level Distal to this level: material constituting
odontoblastic process
Electron Microscope FindingsOdontoblastic process: Dentinal fibers/ Tomes’ fibers Traverses predentin, fills the lumen of
dentinal tubule Coated vesicles: pinocytic & ingest
material from predentin Numerous filaments: parallel to cell
membrane- characteristic
Electron Microscope FindingsIntercellular Junctions: Regions of plasma membranes between
cells 3 types:
Impermeable Adhering
Communicating
Electron Microscope FindingsImpermeable Junctions/ Tight Junctions: Helps: maintain a distinct internal
environment Plasma membranes appear to fuse &
offer a tight seal between cells
Electron Microscope FindingsAdhering Junctions: Maintained by desmosomes:
intercellular bridges 3 types: Belt, Spot & Hemidesmosomes Promote adhesion between cells
Electron Microscope FindingsCommunicating Junctions/ Gap Junctions: Mediate direct transfer of chemical
messages between cells Exchange nutrients & signal molecules
for coordination of function
Gap junction & Tight junction
Desmosome like junction
Sasaki T et al, 1982
Electron Microscope FindingsOdontoblastic Junctional Complexes: Surface epithelial cells: terminal bars at
apical extremities Consist of several components:
junctional complexes Components: Zonula occludens, Zonula
adherens & Macula adherens
Electron Microscope Findings Structures at border between odontoblastic
process & cell bodies: small gap junctions, tight junctions & desmosome like junctions
Tight adhesion between odontoblasts: not easily separated
Electron Microscope FindingsNerve endings: Presence of nerves in tubules:
controversial Nerve endings in juxtaposition to
odontoblastic processes: reported
Electron Microscope FindingsOdontoblastic Communications: Odontoblastic nuclei: inner border of
dentin Odontoblastic processes : adjacent
processes through extensive lateral branch system (Kaye & Herold, 1966)
Contact cells more centrally located: fine protoplasmic processes-
fibronectin: cell to cell adhesion
Electron Microscope Findings Odontoblasts: mesenchymal syncytium-
injury of one odontoblast affects others Continuity of cells lost: injury following
operative procedures Cytoplasm stains for: RNA, lipids,
ALP, ATPase, ACP, non specific esterases, protein
carbohydrate complex : present
Electron Microscope Findings Cell free Zone/ Layer
of Weil: under odontoblasts in coronal portion- nerve elements
Not observed in middle & apical portions (Gotjamanos,1969)
Cell rich Zone: Fibroblasts & undifferentiated mesenchyme cells
Defense cellsHistiocytes and Macrophages: Pericytes : differentiate into fixed or
wandering histiocytes under appropriate
stimulation. Highly phagocytic: remove bacteria,
foreign bodies, dead cells, debris. Pulpal macrophages & dendritic cells:
Langerhans’ cells
Defense cellsPolymorphonuclear Leukocytes: Commonest : pulpal inflammation Injury & cell death: rapidly migrate from
nearby vessels Microabscess formation Bacteria & dead cells. Develop wider zones of inflammation.
Silva et al, 2009
Defense cellsLymphocytes and Plasma Cells: Follows neutrophils. Injury & resultant immune responses Presence of a persistent irritant
Defense cellsMast Cells: Inflamed pulps Granules: histamine & heparin. Histamine: vasodilatation & increases
vessel permeability
Reserve Cells Descendants of undifferentiated cells in
the primitive dental papilla Multipotential cells : Fibroblast type Capable: dedifferentiate/redifferentiate-
mature cell types. Cell-rich zone: concentrations of such
cells.
Reserve Cells Produce little collagen: not mature
fibroblasts (Frank- 1970) Cytoplasmic connections: odontoblasts & subjacent mesenchymal cells (Baume-
1980) Near vessels: other mature cell types Mast cells and odontoclasts:
inflammation.
Reserve Cells Unique cells: calcified tissue - pulp cap/
pulpotomy[Ca(OH)2 ]
Along the calcified tissue: base of tubules involved with caries, restorations, attrition, abrasion
Not a true dentin; cells - not true odontoblasts
Fibers of the Pulp Reticular fibers: around blood vessels &
odontoblasts Collagen- 640 Ao
Type III collagen: 28% to 45%- histologically identified as reticulin
Type I also
Fibers of the Pulp 2 types of filaments Rel. straight, approx 200 Ao diameter &
200 Ao periodicity Coiled, branched & irregularly beaded,
100 Ao diamter
Bernick S
Fibers of the Pulpvon Korff fibers: Fine argyrophilic
fibers Spirally twisted
bundles- cork screw
Unmineralised dentin/ predentin
Fibrillar framework
Fibers of the Pulp Collagen deposition Diffuse: no definite orientation Bundle: large, coarse bundles run parallel
to nerves / independently (Stanley & Ranney, 1962)
Apical portion: more fibrous than coronal (van Amerongen et al, 1983)
Fibers of the Pulp Coronal pulp: more bundle collagen Type III collagen & proteoglycans:
arterial plexus & odontoblasts Extirpation of young cellular pulp:
difficult Aged pulp: like absorbent paper point
Ground substance Structureless mass, gel-like in consistency:
the bulk of the pulp Occupies the space between formed
elements Influences: Spread of infection Metabolic changes Stability of crystalloids Effects of metabolic substances
Ground substance Proteins with glycoproteins, acid
mucopolysaccharidesGAGs: Hyaluronic acid (Engfeldt & Hjerpe, 1972) Water retention Ion Binding Electrolyte distribution during
mineralization Collagen fibrillogenesis
Ground substance ‘Milieu interieur’: Engel (1958) Metabolites & breakdown products-
exchange Hyaluronic acid: metabolite transport
Ground substance Pulp tissue hydroatatic pressure: 15 mm
Hg increase- early stages of inflammation
Depolymerization: microbial enzymes change in ground substance
Hyaluronidases, chondroitin sulfatase Mucopolysaccharidase activity:
resorbing deciduous teeth
Circulation of the Pulp Systemic circulation Microcirculation Lymphatics Control of blood flow Transcapillary fluid exchange Circulation in the inflamed pulp Clinical correlations
Arterial blood supply to teethRight atrium
Right ventricle
Pulmonary artery
Lungs
Pulmonary vein
(left ventricle)
Aorta
CCA
ECA Internal Maxillary artery
Internal Maxillary artery
pterygopalatinepterygoidmandibular
Inferior alveolar
Dental branch
Lower Molars, premolars canines
Incisive branch
Lower Incisors
Infraorbital artery
ASA artery
PSA artery
Upper Incisors, canines
Upper molars
bicuspids
Venous drainageNasopalatine, infraorbital, descending palatine, PSA, pharyngeal, Deep temporal, masseteric, Inferior alveolar, Middle meningeal
Pterygoid venous plexus
Internal maxillary vein with superficial temporal vein
Retromandibular vein
EJV/ IJV Innominate vein(right side)
Superior venacava
Heart (Right atrium)
Microcirculation Arterioles, capillaries & venules Arterioles: 50μ diameter: enter
through apical foramen Branch : terminal arterioles capillary
plexus – subodontoblastic zone Young teeth: extend into odontoblastic
layer
Arteriovenous distribution of hemodynamics in rat dental pulp S. Kim et
al, 1984
Takahashi et al- 1982
Microcirculation Capillaries: 8 to
10μ Coronal portion:
capillary blood flow- twice that in the root
Pulp horns: greatest blood flow
Dr. K. Josephsen, Denmark
Microcirculation Fenestrations:
rapid transport of fluid & metabolites
Avg. capillary density: 1400/ mm3 : the greatest in the body
Microcirculation Capillary plexus
Postcapillary venules
Larger venules
Arteriovenous anastomosis: sympathetic innervation
Takahashi et al, 1982
Arteriole distribution Main arteriole- 2
groups Coronally – pulp
horn Between roof and
floor of pulp chamber
Microcirculation Pulpal venules: unusually thin walls,
discontinuous muscular layer Diameter maximum: central region-
200μ Resting pulpal blood flow: 0.15 to 0.6
ml/ min/g tissue Blood volume: 3% pulpal wet weight
Microcirculation Changes measured: Laser Doppler
flowmeters Detect revascularization: traumatized
teeth Ideal : pulp vitality Limited: sensitivity, specificity,
reproducibility & costs
Regulation of pulpal blood flow Neuronal, paracrine & endocrine
mechanisms Vasodilatation: neighboring tissues- drop
in pulpal blood flow & perfusion pressure
Pulp: vulnerable in gingivitis/ periodontitis
Neuronal regulation Little/ no sympathetic vasoconstrictor
tone Neuronal vasodilator tone: sensory
neuropeptides Cervical sympathetic trunk:
vasoconstriction Neuropeptide Y & norepinephrine
Neuronal regulation Blood flow sensory neuropeptides
Vasodilatation : CGRP release Muscarinic receptors: ACh & VIP –
vasodilatation (Yu CY et al- 2002) No parasympathetic vasodilatation: cat
pulp (Sassano et al- 1995)
Local control Local tissue demands: regulate
hemodynamics Endothelin-1 pulpal blood flow
Prostacyclin, NO : endothelium Adenosine: ischemic & hypoxic tissue-
low pulpal oxygen tension
Humoral control Angiotensin II : vasoconstrictive basal
tone Receptors: AT1, AT2- rat pulp (Souza PP
et al, 2007) DOPA, epinephrine: vasoconstriction ACh, Histamine, bradykinin : inhibit
vasoconstriction
Lymphatics Drains filtered fluids & proteins: returns
to blood Immune defense Lymphatic markers: extensive lymphatic
system in pulp Capillaries- pulp horns; leave via apical
foramen & lateral canals
From Berggreen E, Haug SR, Mkonyi LE, Bletsa A: Characterization of the dental lymphatic system and identification of cells immunopositive to specific lymphatic markers. Eur J Oral Sci 117(1):34–42, 2009
Lymphatics Arteriolar pulse pressure
High interstitial pulsatile pressure
Deformation of interstitial tissues
Propulsion of lymph
Lymphatic drainage of teethAll maxillary teeth, Mandibular canines, premolars & molars
Mandibular incisors
Submaxillary glands
Submental glands
Superficial & deep cervical glands
Thoracic duct (left)
Jugular duct (right)
Blood stream: junction of IJV & Subclavian veins
Transcapillary fluid exchange Regulated by : lymph flow & differences
in colloidal osmotic & hydrostatic pressures
Interstitial fluid volume: 0.6+ 0.03 ml/g
Interstitial fluid pressure: 6- 10 mm Hg COP: rel. high- 83% plasma COP
Wiig H, Rubin K, Reed RK: New and active role of the interstitium in control of interstitial fluid pressure: potential therapeutic consequences. Acta Anaesthesiol Scand 47:111–121, 2003.
Circulation in the inflamed pulp Inflammation: vasodilatation &
increased vascular permeability- interstitial fluid pressure
Reabsorption of tissue fluid: pressure- disproves Pulpal strangulation theory (Heyeraas & Berggreen- 1999, Heyeraas & Kvinnsland- 1992)
Circulation in the inflamed pulp PGE2, Bradykinin, SP, Histamine: pulpal
blood flow Serotonin: pulpal blood flow Acute inflammation: 200% of control flow &
increased vascular permeability (Heyeraas & Kvinnsland- 1992, Heyeraas et al- 1996)
LPS: circulatory dysfunction (Bletsa A et al, 2006)
Circulation in the inflamed pulp Endothelial perturbation: on
exposure to endotoxin/ cytokines Reduced perfusion, VEGF down
regulation & microvessel density : necrosis
Lymphangiogenesis : inflamed pulps(Pimenta et al, 2003)
Vascular permeability: Inflamed pulp Vascular leakage: Prostaglandin,
histamine, bradykinin, SP LPS, LTA, TNF-, IL-1: upregulate VEGF
vascular permeability protein Transport COP
Circulation in the inflamed pulp: Clinical aspect Reduced distractions at night Pulpal blood flow : supine Further pulpal tissue pressure:
activate sensitised nociceptors- spontaneous pain
Throbbing : pulsations in the pulp - systole
Clinical correlationsLOCAL ANESTHETICS: Blood flow infiltration : LA +
epinephrine
Pulp tissue pressure high conc. Vasoconstrictors (Van Hassel & Simard- Savoie et al 1973)
No serious/ permanent damage
Clinical correlationsGENERAL ANESTHETICS: Scott et al – 1972: rat study- pulpal
blood flow velocity: zero in 30 seconds Effects: disappear in 1 hourAGING: Decreased circulation Atherosclerotic changes: calcification Cells atrophy & die; fibrosis
Clinical correlationsTEMPERATURE CHANGESElevation: 100C to 150C increase: intrapulpal
pressure 2.5mm Hg/0C
Irreversible changes: heating to 450C- prolonged (Van Hassel & Brown- 1969)
Clinical correlations Tooth preparation: affect pulpal blood
flow Pulpal damage initiation: alteration in
microvasculature No water spray: reduced blood flow-
upto 1 hour (Kim et al, 1983)
Clinical correlationsReduction: Subfreezing temperatures: transient fall Intrapulpal pressure (Augsburger & Peters- 1981) < -20C: vascular engorgement &
necrosis H2O2 & CO2 : reduce capillary blood
flow
Clinical correlationsENDODONTIC THERAPY: Less hemorrhage: extirpation close to
apexDEVELOPMENT: Blood vessel density increased coronally Subodontoblastic capillary plexus- larger
: eruption Rich blood supply- floor of pulp chamber
Seltzer et al, 1963
Clinical correlationsPERIODONTAL DISEASE: Reduction- circulation:
degenerative changes Reparative processes
diminished: older pulps: operative procedures- necrosis
Excessive irradiation: necrosis
Clinical correlationsANTERIOR OSTEOTOMY: Blood flow: maximum decrease
immediate postop Apparently re established: normal
response to stimuli (Pepersack- 1973, Theisen & Guernsey- 1976)
Nerve supply of the pulp Innervation of the teeth Theories of tooth pain perception Modulation of nerve impulses
Innervation of the teethVth N
Ophthalmic Maxillary Mandibular
PSA Infraorbital
ASA Lingual Inferior alveolar
Maxillary molars
Maxillary premolars
Maxillary anteriors
Inferior dental Incisor
Mandibular molars and premolars
Mandibular cuspid and incisors
Convergence of sensory information : teeth to higher centres
Innervation Large no. of myelinated (A)&
unmyelinated (C) fibers Premolar: 2000 Not all are nociceptors Afferent: sensory Efferent: Sympathetic: circulation & eruption
Characteristics of sensory fibers
FiberMyelination
Location of Terminals
Pain Characteristics
Stimulation Threshold
A-delta YesPrincipally in region of pulp-dentin junction
Sharp, prickingRelatively low
C No
Probably distributed throughout pulp
Burning, aching, less bearable than A-delta fiber sensations
Relatively high, usually associated with tissue injury
Sensory fibers Aδ: 1-5μ; 6-30 m/s C: 0.4-1μ; 0.5-2 m/s Pain localization: Single neuron innervation Low density propioceptors Electrical stimulation: A fibers
Fiber location within pulp
Sensory fibersNerve bundles +blood vessels
Dr. Inge Fristad, Department of Clinical Dentistry, University of Bergen
Plexus of Raschkow
Mummery - 1919 Plexus of single nerve
axons Develop: final stages of
root formation Prolific branching:
overlapping receptor fields
A fibers: subodontoblastic plexus
Terminal axons: free nerve endings
Types of nerve endings: Gunji T- 1982
Odontoblasts: receptor?? No anatomic communication: nerve fibers Low membrane potential: -24 to -30 mV Disruption of layer: no sensitivity
Possibility: sodium channel activity/ factors release- neuromodulation
Nerve fibers: resist necrosis Noxious stimuli: periapical tissues
Pain in non vital
teeth
Tissue injury & deafferentiation Deafferentiation: regeneration/ neuronal
cell death V nuclei affected: pulp extirpation Phantom tooth pain Changes in gene expression: C-fos
(Byers et al, 1993) A fibers: thermal & electric tests C fibers: pulp injured
Theories of tooth pain perception Dentinal nerve
stimulation Dentinal receptor
theory Hydrodynamic
theory
Dentinal nerve stimulation Silver staining: controversial LM studies: variable penetration (Bernick-
1968) & termination (Rapp et al- 1957)
EM studies: difficult interpretation No connection: nerves & odontoblasts
(Fernehead – 1968)
Dentinal nerve stimulation Predentin:
associated cells- origin questioned (Arwill- 1967)
Arwill T
Dentinal nerve stimulation Axons: separated
by narrow cleft (Byers et al)
Nerves: beaded structures in SEM (Tidmarsh- 1981)
Frank RM
Dentinal nerve stimulation Frank et al- 1966
Nerve : concavity odontoblast(pic)
‘cork screw’ fibers Gap junctions: nerve
cell processes & odontoblasts (Holland- 1975)
Possible- no nerve connections
Dentinal receptor theory Odontoblasts & processes: receptor Inconclusive Evidence: recording electrical activity Heat, cold, touch receptors (Scott &
Tempel, Mumford- 1965) Electrical activity: nerves in pulp & not
dentin (Matthews- 1970)
Dentinal receptor theory Intradentinal receptor: connections
between odontoblastic process & nerve fiber (Frank- 1969)
Transducer mechanism AChE: demonstrated in several studies
(Avery and Rapp-1967); contrary too Adrenergic : pulpal blood vessel walls
Hydrodynamic theory Dentin pain & odontoblast
displacement: related BrӓnstrӦm et al (1966, 1967, 1969,
1972) and Lilja (1980): hydrodynamic mechanism
Hydrodynamic theory Stimuli:
expansion/ contraction – fluid
Pulpward/ outward movement: nerve stimulation
Hydrodynamic theoryMechanisms - reduce fluid flow in dentin: Pashley et al- 1982 Plaque/ saliva bacteria Mineralized deposits- tubules Salivary/plasma proteins
Hydrodynamic theory- hypersensitive dentin4 treatment modalities: Smear layer- burnishing root surface Oxalate compounds: insoluble ppts in
tubules Tubule occlusion: pptd. Plasma proteins-
HEMA + glutaraldehyde Dentin bonding agents applicationLASER : effects on pulp???
Pulpal tissue pressure & pain Blood flow, pressure changes, dental
pain hydrostatic pressure: nerve fiber stimulation (Nӓhri- 1978) Pulp: mechanoreceptor- pain
transmission
Polypeptides & NeurotransmittersPLASMA KININS: No pain: application to dentin
( Anderson and Naylor- 1972)0SUBSTANCE P: Pulp: rich in SP Vasodilatation , increased capillary
permeability (Pashley et al- 1982)
Polypeptides & NeurotransmittersPROSTAGLANDINS: Sensitize nociceptors: histamine,
bradykinin, SP CGRP, Neuropeptide Y, NKA, VIP: painful pulps/ beneath caries Vasodilatation SP, CGRP: wound healing, inflammation CGRP release: vasoconstrictors
Systemic factors Vitamin deficiency Hormones Protein deficiency Systemic virus infection Hereditary diseases Tumor metastases
Vitamin deficiency
Vitamin C- Fibroblasts- Odontoblasts: degenerate & lose
morphology
Hormones & hormonal imbalanceSteroids: Systemic corticosteroid Odontoblasts Inhibit reparative dentinogenesis Steroid :pulp therapy???
Hormones & hormonal imbalanceDiabetes mellitus: Glucose concentration rise in dentinal
pulp fluids Degenerative & inflammatory changes
in pulp Dentinogenesis affected Atrophic pulp: non carious teeth Acute inflamed pulp: carious teeth
Cohen et al, 1963
Hormones & hormonal imbalanceThyroid deficiency: Pulp vascularity Pulpal lumen Cellular elements
Protein deficiency No pulpal changes noted (Glickman &
Shklar- 1954) Larger areas of periapical rarefaction
( Stahl et al -1958)
Systemic virus infection Odontoblasts injured: lymphocytic
choriomeningitis (Hancock-1956) & Shope papilloma virus ( Fleming-1958)
Degenerative changes & eventual necrosis: rats with Polyoma virus
Hereditary diseases Blood: Sickle cell anemia, leukemia Reticulo endothelial system: Hand-
SchÜller- Christian disease Neurologic: Sturge- Weber disease Metachromatic leukodystrophy Krabbe’s leukodystrophy Fabry’s disease Niemann- Pick disease
Tumor transplantation Metastases: sparse reports Epitheliomas, sarcoma, Burkitt’s
lymphomas- human dental pulps (Stanley- 1973)
Dental Pulp Stem Cells (DPSCs) Gronthos et al – 2000 Osteo/ odontogenic, adipogenic,
neurogenic, chondrogenic, myogenic Tissue regeneration DPSCs: dentinal repair Appropriate carrier: dental implant
Courtesy:
DPSCs DPSCs+ collagen + DMP1: pulp like
tissue (Prescott et al, 2008) SHED: dental pulp tissue engg (Cordeiro
et al,2008) Serum free medium + Insulin-
transferrin- selenium- X & embryotrophic factor: suitable medium for culture (Hirata et al, 2010)
DPSCs Irreversible pulpitis: putative cells- stem
cell properties (Wang et al, 2010) Regeneration in canine teeth – Gelfoam
scaffold (Wang et al- 2013)
Conclusion Unique tissue Resembles embryonic connective tissue Dynamic response pattern
References Seltzer S, Bender J.B. Seltzer’s The
Dental Pulp. Biological considerations in dental procedures. 3rd Edition
Hargreaves KM, Cohen S. Cohen’s Pathways of the Pulp. 10th Edition
Ingle JI, Bakland LK. Ingle’s Endodontics. 5th Edition
References Gronthos S, Mankani M, Brahim J, Gehron
Roby P, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. PNAS 2000; 97(25): 13625- 13630
In Vivo Generation of Dental Pulp-like Tissue by Using Dental Pulp Stem Cells, a Collagen Scaffold, and Dentin Matrix Protein 1 after SubcutaneousTransplantation in Mice. Prescott RS, Alsanea R, Fayad MI et al. J Endod 2008;34:421– 426
References Cordeiro MM, Dong Z, Kaneko T et al.
Dental Pulp Tissue Engineering with Stem Cells from Exfoliated Deciduous Teeth. J Endod 2008;34:962–969
Wang Z, Pan J, Wright JT et al. Putative Stem Cells in Human Dental Pulp with Irreversible Pulpitis: An Exploratory Study. J Endod 2010;36:820–825)
References Hirata TM, Ishkitiev N, Yaeigaki K et al.
Expression of Multiple Stem Cell Markers in Dental Pulp Cells Cultured in Serum-free Media. J Endod 2010;36:1139–1144
Wang Y, Zhao Y, Jia W, Yang J, Ge L. Preliminary Study on Dental Pulp Stem Cell–mediated Pulp Regeneration in Canine Immature Permanent Teeth. J Endod 2013;39:195–201
References
Kim S, Lipowsky HH, Usami S, Chien S. Arteriovenous Distribution of Hemodynamic Parameters in the Rat Dental Pulp. Microvasc Res 27, 28-38 (1984)
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