Differential diagnosis of brucellosis serologic reactions
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Transcript of Differential diagnosis of brucellosis serologic reactions
Differential Diagnosis of Brucellosis Serologic Reactions
Steven G. HennagerBacterial and Hemoparasitic Serology Team
National Veterinary Services Laboratories
Workshop: An Integrated Approach to Controlling Brucellosis in Africa, Addis Ababa, 29-31 January 2013
Safeguarding Animal Health
Dominant Brucella Antigens
Species • Brucella melitensis• Brucella abortus• Brucella suis• Brucella canis• Brucella ovis
Primary Antigen • M and A• A and M• A and M• Rough• Rough
Blue colonies = Smooth Brucella sp.Crystal colonies = Rough Brucella sp.
Safeguarding Animal Health
Immune response to Brucella infection
• Cell mediated response
• Humoral or antibody production
Safeguarding Animal Health
Type: JPG
Website for this image... and faster response - antibody titre is much higher, occurs much faster, ...classes.midlandstech.edu•Full-size image
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Safeguarding Animal Health
Serologic Assays Antibody detection
Serum Milk
Rapid Inexpensive Sensitivity and Specificity Assays can be impacted by
Vaccination Environmental conditions
Brucella card test
Brucellosis Diagnostic Tests
Brucellosis Diagnostic Tests
Safeguarding Animal Health
Brucellosis Diagnostic Tests
Brucellosis Diagnostic Tests
Brucellosis Diagnostic Tests
Brucellosis Diagnostic Tests
Other Brucella Diagnostic Tests
• Polymerase Chain Reaction (PCR)• Culture Identification• Western Blot
Amount of Isotype (ng)
Test IgM IgG1 IgG2 IgA
Tube 20 ----- 125 650
Card ----- 600 7500 -----
Riv ----- 1550 2750 -----
CF 6500 290 ----- -----
ELISA 155 190 220 700
Brief History 19th century – Bruce isolated organism
from human spleens Linked organism to raw goat’s milk Genus named after him
19th century – Bang discovered organism that caused abortions in cattle Named organism Bacillus abortus
20th century – Evans recognized the relationship between Bruce’s organism and Bang’s organism Advocated pasteurization of milk Re-named organism Brucella abortus
Sir David Bruce
L.F. Bernhard Bang
Alice C. Evans
Acknowledge Superior Laboratory Support
• De Anne Wall, Diane Davis, Barb Kesl, Molly Fritz– Serology Laboratory Technicians
Questions?