Development of Biosensors to Detect Biological Agents in Water
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Transcript of Development of Biosensors to Detect Biological Agents in Water
Development of Biosensors to Detect
Biological Agents in Water Melissa Bui
Mentor: Dr. Christopher ChoiAgricultural and Biosystems EngineeringCollege of Agriculture and Life SciencesUniversity of Arizona/NASA Space Grant
2007-2008 Statewide SymposiumApril 19, 2008
Presentation Outline
The Issue at HandBenefits of using biosensorsMethodsKey Findings
The Issue at Hand
Arizona’s water distribution network is comprised of a grid of pipes
Entry points of the grid make it easy for contaminants to pollute the water
There is currently no real-time method for detecting contaminants in Arizona’s water supply
Benefits of using biosensors
Microfluidic device provides a faster method of detecting microbes than current methods (assays)
Uses less materials than traditional culture plate method
Faster detection Eliminating the problem before it affects the masses
Can be used to prevent bioterrorism
MethodsE. Coli was the microbe being detected Salt injected in 8.5 m pipe at a flow rate of 0.4
L/m for 10 seconds 1 mL of E. Coli (concentration greater than 109
CFU/mL) in 1 L dechlorinated water is injected Samples taken in set time intervals with a
fraction collector at downstream end of pipe Samples taken at critical time points were
analyzed using biosensor and cell culturing
Comparison of optical signals to cell counts and salt tracer data
Turbulent flow Laminar flow
Jin-Hee Han, PhD Student, University of Arizona
E. Coli counts and microfluidic signals
Jin-Hee Han, PhD Student, University of Arizona
Key Findings
Microfluidic signals showed same trends as E. Coli counts and salt tracer data
Data from culture counts and sensors signals corresponded after normalizing data
Detection limit of biosensor as low as 10 CFU/mL
Sensors detected sample concentration in 5 minutes, while culturing samples took at least 2 days
AcknowledgementsSupport provided by: Dr. Christopher
Choi, Dr. Jeong-Yool Yoon, Jin-Hee Han, and Dr. Ryan Sinclair
Figures courtesy of Jin-Hee Han