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Curriculum Vitae for Academic Promotion The Johns Hopkins University School of Medicine

_____________________ __December, 2019____ Larissa A. Shimoda Date DEMOGRAPHIC AND PERSONAL INFORMATION

Current Appointment 2015-present Professor of Medicine, Division of Pulmonary and Critical Care Medicine,

Johns Hopkins University School of Medicine

Personal Data Johns Hopkins University Department of Medicine Division of Pulmonary and Critical Care Medicine JHAAC 4B.82B 5501 Hopkins Bayview Circle Baltimore, MD 21224 (410) 550-5355 FAX (410) 550-2612 Electronic Mail: lshimod1@jhmi.edu

Education and Training 1990 Bachelor of Science in Engineering, Marquette University, Biomedical Engineering 1992 Master of Science, Marquette University, Biomedical Engineering 1995 Doctor of Philosophy, Marquette University, Biomedical Engineering 1998 Postdoctoral Fellow, Johns Hopkins University, School of Medicine, Division of Pulmonary and Critical Care

Medicine

Professional Experience: 1998-1999 Research Associate, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, School of

Medicine 1999-2000 Instructor of Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, School

of Medicine 2000-2007 Assistant Professor of Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins

University, School of Medicine 2007-2015 Associate Professor of Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins

University, School of Medicine 2014- Director, Basic Research, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University,

School of Medicine 2015- Professor of Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, School

of Medicine RESEARCH ACTIVITIES Peer Reviewed Original Science Publications 1. Shimoda LA, Norins NA, Jeutter DC, Madden JA. Flow-induced responses in piglet isolated cerebral arteries. Pediatr

Res 39: 574-583, 1996.

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2. Shimoda LA, Norins NA, Madden JA. Flow-induced responses in cat pulmonary arteries. J Appl Physiol 83: 1617-1622, 1997.

3. Shimoda LA, Norins NA, Madden JA. Responses to pulsatile flow in piglet isolated cerebral arteries. Pediatr Res 43: 514-520, 1998.

4. Shimoda LA, Sylvester JT, Sham JSK. Endothelin-1 inhibits delayed rectifier potassium (K+) current in rat intrapulmonary arterial smooth muscle cells. Am J Physiol 274: L842-L853, 1998.

5. Yu AY, Frid MS, Shimoda LA, Stenmark KR, Wiener CM, Semenza GL. Temporal, spatial and oxygen-regulated expression of hypoxia-inducible factor 1 in the lung. Am J Physiol 275: L818-826, 1998.

6. Yu AY, Shimoda LA, Iyer NV, Sun X, McWilliams R, Beaty T, Wiener CM, Sylvester JT, Semenza GL. Impaired physiologic responses to chronic hypoxia in mice partially deficient for hypoxia-inducible factor 1a. J Clin Invest 103: 691-696, 1999.

7. Shimoda LA, Sylvester JT, Sham JSK. Chronic hypoxia alters the effect of endothelin and angiotensin on K+ currents in pulmonary arterial myocytes. Am J Physiol 277: L431-L439, 1999.

8. Shimoda LA, Sylvester JT, Sham JSK. Mobilization of intracellular Ca2+ by ET-1 in rat intrapulmonary arterial smooth muscle cells. Am J Physiol 278:L157-164, 2000.

9. Shimoda LA, Sham JSK, Sylvester JT. L-type Ca2+ channels, resting Ca2+ and ET-1-induced responses in chronically hypoxic pulmonary myocytes. Am J Physiol 279 L884-L894, 2000.

10. Sham JSK, Crenshaw BR, Shimoda LA, Sylvester JT. Effects of hypoxia in porcine pulmonary arterial myocytes: roles of KV channel and endothelin-1. Am J Physiol 279:L262-72, 2000.

11. Shimoda LA, Sylvester JT, Booth GM, Shimoda TH, Undem BJ, Sham JSK. Inhibition of voltage-gated K+ currents by endothelin-1 in human pulmonary arterial myocytes. Am J Physiol Lung Cell Mol Physiol 281: L1115-22, 2001.

12. Shimoda LA, Manalo DJ, Sham JSK, Semenza GL, Sylvester JT. HIF-1alpha deficiency impairs pulmonary arterial myocyte electrophysiological responses to hypoxia. Am J Physiol Lung Cell Mol Physiol 281:L202-8, 2001.

13. Lui Q, Sham JSK, Shimoda LA, Sylvester JT. Hypoxic constriction of porcine distal pulmonary arteries: endothelium- and endothelin-dependence. Am J Physiol Lung Cell Mol Physiol 280:L856-65, 2001.

14. Shimoda LA, Welsh L, Pearse DB. Inhibition of inward rectifier K+ channels by cGMP in pulmonary endothelial cells. Am J Physiol Lung Cell Mol Physiol. 283: L297-304, 2002.

15. Remillard CV, Zhang WM, Shimoda LA, Sham JSK. Physiological properties and functions of Ca2+ sparks in rat intra-pulmonary arterial smooth muscle cells. Am J Physiol Lung Cell Mol Physiol. 283: L433-44, 2002.

16. ZhangWM, Yip K-P, Lin MJ, Shimoda LA, Li W-H, Sham JSK. Endothelin-1 activates Ca2+ sparks in pulmonary arterial smooth muscle cells: local Ca2+ signaling between ryanodine and inositol triphosphate receptors. Am J Physiol Lung Cell Mol Physiol 285: L680-90, 2003.

17. Liu Q, Sham JSK, Shimoda LA, Kuppasamy R, Sylvester JT. Hypoxic constriction and reactive oxygen species in porcine distal pulmonary arteries. Am J Physiol Lung Cell Mol Physiol 285: L322-33, 2003.

18. Pearse DB, Shimoda LA, Verin AD, Bogatcheva N, Moon C, Ronnett GV, Welsh LE, Becker PM. Effect of cGMP on lung microvascular endothelial barrier dysfunction following hydrogen peroxide. Endothelium. 10(6):309-17, 2003.

19. Hassoun PM, Filippov G, Fogel M, Donaldson C, Kayyali US, Shimoda LA, Bloch, KD. Hypoxia decreases soluble guanylate cyclase expression in pulmonary artery smooth muscle cells. Am J Respir Cell Mol Biol. 30: 908-13, 2004.

20. Wang J, Shimoda LA, Sylvester JT. Capacitative calcium entry and TRPC channel proteins are expressed in rat distal pulmonary arterial smooth muscle. Am J Physiol Lung Cell Mol Physiol. 286(4):L848-58, 2004.

21. Wang J, Shimoda LA, Weigand LA, Wang W, Sun D-J, Sylvester JT. Acute hypoxia increases intracellular [Ca2+] in pulmonary arterial smooth muscle by enhancing capacitative Ca2+ entry. Am J Physiol Lung Cell Mol Physiol 288(6):L1059-69, 2005.

22. Wang J, Weigand LA, Wang W, Sylvester JT, Shimoda LA. Chronic hypoxia inhibits Kv channel gene expression in rat distal pulmonary artery. Am J Physiol Lung Cell Mol Physiol 288(6):L1049-58, 2005.

23. Weigand L, Foxson J, Wang J, Shimoda LA, Sylvester JT. Inhibition of hypoxic pulmonary vasoconstriction by store-operated Ca2+ channel antagonists. Am J Physiol Lung Cell Mol Physiol 289: L5-L13, 2005.

24. Rios EJ, Fallon M, Wang J, Shimoda LA. Chronic hypoxia elevates intracellular pH and activates Na+/H+ exchange in pulmonary arterial smooth muscle cells. Am J Physiol Lung Cell Mol Physiol 289: L867-74, 2005.

25. Campen MJ, Shimoda LA, O’Donnell CP. The acute and chronic cardiovascular effects of intermittent hypoxia in C57BL/6J mice. J Appl Physiol 99: 2028-35, 2005.

26. Weigand LA, Sylvester JT, Shimoda LA. Mechanisms of endothelin-1-induced contraction in pulmonary arteries from chronically hypoxic rats. Am J Physiol Lung Cell Mol Physiol 290:L294-90, 2006.

27. Moldobaeva A, Welsh LE, Shimoda LA, Verin AD, Tuder RM, Pearse DB. Role of protein kinase G in barrier

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protective effects of cGMP in Human pulmonary artery endothelial cells. Am J Physiol Lung Cell Mol Physiol 290: L919-30, 2006.

28. Wang, J. Weigand L, Sylvester JT, Semenza GL, Shimoda, LA. HIF-1 mediates hypoxia-induced TRPC expression and elevated intracellular Ca2+ in pulmonary arterial smooth muscle cells. Circ Res 98:1528-37, 2006.

29. Zuckerbraun BS, Billiar TR, Chin BY, Wegiel B, Billiar TR, Czsimadia E, Rao J, Shimoda L, Ifedigbo E, Kanno S, Otterbein LE. Carbon monoxide reverses established pulmonary hypertension. J Exp Med 203(9):2109-19, 2006.

30. Shimoda LA, Wang J, Sylvester JT. Chronic hypoxic and calcium channels. Microcirculation 13(8):657-70, 2006. 31. Shimoda LA, Luke T, Sylvester JT, Shih HW, Jain A, Swenson ER. Inhibition of hypoxia-induced calcium responses

in pulmonary arterial smooth muscle by acetazolamide is independent of carbonic anhydrase inhibition. Am J Physiol Lung Cell Mol Physiol 292(4):L1002-12, 2007.

32. Grigoryev DN, Ma SF, Shimoda LA, Johns RA, Lee B, Garcia JG. Exon-based mapping of microarray probes: Recovering differential gene expression signal in underpowered hypoxia experiment. Mol Cell Probes. 21(2):134-9, 2007.

33. Girgis RE, Mozammel S, Champion HC, Li D, Peng A, Shimoda L, Tuder RM, Johns RA, Hassoun PM. Regression of chronic hypoxic pulmonary hypertension by simvastatin. Am J Physiol Lung Cell Mol Physiol 292(5):L1105-10, 2007.

34. Fukuda R, Zhang H, Kim J-W, Shimoda LA, Dang CV, Semenza GL. HIF-1 regulates cytochrome oxidase subunit composition to optimize efficiency of respiration in hypoxic cells. Cell 129(1):111-22, 2007.

35. Wang J, Weigand L, Foxson J, Shimoda LA, Sylvester JT. Ca2+ signaling in hypoxic pulmonary vasoconstriction: effects of myosin light chain and rho kinase antagonists. Am J Physiol Lung Cell Mol Physiol 293: L674-85, 2007.

36. Whitman EM, Pisarcik S, Luke T, Fallon M, Wang J, Sylvester JT, Semenza GL, Shimoda LA. Endothelin-1 mediates hypoxia-induced inhibition of voltage-gated K+ channel expression in pulmonary arterial myocytes. Am J Physiol Lung Cell Mol Physiol 294: L309-18, 2008.

37. Zaiman AL , Podowski M, Gordy K, Zhen L, Shimoda LA , Neptune E, Medicherla S, Protter A, Champion HC, Tuder RM. Role of TGF-b in pulmonary vascular remodeling associated with pulmonary hypertension. Am J Resp Crit Care Med 177: 896-905, 2008.

38. Sidhaye VK, Schweitzer K, Caterina M.J, Shimoda LA, King LS. Shear stress regulates AQP5 and airway epithelial barrier function. Proc.Natl.Acad.Sci 105: 3345-50, 2008.

39. Zhang H, Bosch-Marce M, Shimoda LA, Tan YS, Hyen Baek J, Wesley JB, Gonzalez FJ, Semenza GL. Mitochondrial autophagy is a HIF-1-dependent adaptive metabolic response to hypoxia. J Biol Chem 283: 10892-10903, 2008.

40. Lu W, Wang J, Shimoda LA, Sylvester JT. Differences in STIM1 and TRPC expression in proximal and distal pulmonary arterial smooth muscle are associated with differences in Ca2+ responses to hypoxia. Am J Physiol Lung Cell Mol Physiol 295: L104-13, 2008.

41. Guo J, Lu W, Shimoda LA, Semenza GL, Georas SN. Enhanced interferon-gamma gene expression in T cells and reduced ovalbumin-dependent lung eosinophilia in hypoxia-inducible factor-1-alpha-deficient mice. Int Arch Allergy Immunol. 149(2): 98-102, 2009.

42. Roman J, Rangasamy T, Guo J, Sugunan S, Meednu N, Packirisamy G, Shimoda L, Golding A, Semenza G, Georas SN. T cell activation under hypoxic conditions enhances interferon-gamma secretion. Am J Respir Cell Mol Biol. 42(1):123-8, 2010.

43. Lu W, Wang J, Peng G, Shimoda LA, Sylvester JT. Knockdown of stromal interaction molecule 1 attenuates store-operated Ca2+ entry and Ca2+ responses to acute hypoxia in pulmonary arterial smooth muscle. Am J Physiol Lung Cell Mol Physiol. 297(1):L17-25, 2009.

44. Shimoda LA, Fellner S, Swenson ER. Hypoxia-induced Ca2+ responses in spiny dogfish shark (Squalus acanthias) vessels. MDIBL Bull. 48: 20-22, 2009.

45. Polotsky VY, Savransky V, Bevans-Fonti S, Li J, Grigoryev DN, Shimoda LA. Intermittent and sustained hypoxia induce similar changes in the gene expression profile in the human aortic endothelial cells. Physiol Genomics 41: 306-314, 2010.

46. Shimoda LA, Undem C. Interactions between calcium and reactive oxygen species. Respiratory Physiology & Neurobiology 174(3):221-9, 2010.

47. Rey S, Luo W, Shimoda LA, Semenza GL. Metabolic reprogramming by HIF-1 promotes the survival of bone marrow-derived angiogenic cells in ischemic tissue. Blood 117(18):4988-98, 2011.

48. Dang EV, Barbi J, Yang HY, Jinasena D, Yu H, Zheng Y, Bordman Z, Fu J, Kim Y, Yen HR, Luo W, Zeller K, Shimoda L, Topalian SL, Semenza GL, Dang CV, Pardoll DM, Pan F. Control of T(H)17/T(reg) balance by

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hypoxia-inducible factor 1. Cell 146(5):772-84, 2011. 49. Weigand LA, Shimoda LA, Sylvester JT. Enhancement of myofilament calcium sensitivity by acute hypoxia in rat

distal pulmonary arteries. Am J Physiol Lung Cell Mol Physiol 301(3):L380-7, 2011. 50. Zaiman AL, Damico R, Thoms-Chesley A, Files DC, Kesari P, Johnston L, Swaim M, Mozammel S, Halushka M, El-

Haddad H, Shimoda LA, Peng C, Hassoun P, Champion HC, Kitsis RN, Crow MT. A Critical Role for the Antiapoptotic Protein ARC (Apoptosis Repressor with CARD) in hypoxia-induced pulmonary hypertension. Circulation 124(23):2533-42, 2011.

51. Shimoda LA, Semenza GL. HIF and the lung. Am J Resp Crit Care Med 183(2):152-6, 2011. 52. Shimoda LA, Polak J. Hypoxia and ion channel function. Am J Physiol Cell 300(5):C951-67, 2011. 53. Abud EM, Maylor J, Undem C, Zaiman AL, Myers AC, Sylvester JT, Semenza GL, Shimoda LA. Digoxin inhibits

development of hypoxic pulmonary hypertension in mice. Proc.Natl.Acad.Sci 109: 1239-1244, 2012. 54. Suson KD, Stec AA, Shimoda LA, Gearhart JP. Characterization of exstrophy bladder smooth muscle cells in

culture. J Urol 188(4 Suppl):1521-7, 2012. 55. Suson KD, Stec AA, Gearhart JP, Shimoda LA. Transforming growth factor beta 1 mediates migration in cultured

human control and exstrophy bladder smooth muscle cells. J Urol 188(4 Suppl):1528-34, 2012. 56. Luke T, Maylor J, Undem C, Sylvester JT, Shimoda LA. Kinase dependent activation of voltage-gated Ca2+ channels

by ET-1 in pulmonary arterial myocytes during chronic hypoxia. Am J Physiol Lung Cell Mol Physiol 302(10):L1128-39, 2012.

57. Wang J, Shimoda LA, Sylvester, JT. Ca2+ responses of pulmonary arterial myocytes to acute hypoxia require Ca2+ release from both ryanodine and inositol trisphosphate receptors. Am J Physiol Lung Cell Mol Physiol 303(2):L161-8, 2012.

58. Leggett K, Maylor J, Undem C, Lai N, Lu W, Schweitzer K, King LS, Myers AC, Sylvester JT, Sidhaye V, Shimoda LA. Hypoxia-induced migration in pulmonary arterial smooth muscle cells requires calcium-dependent upregulation of aquaporin 1. Am J Physiol Lung Cell Mol Physiol 303(4):L343-53, 2012.

59. Undem C, Rios EJ, Maylor J, Shimoda LA. Endothelin-1 induces Na+/H+ exchanger activity in pulmonary arterial smooth muscle cells via Rho kinase. PLOS One 7(9):e46303, 2012.

60. Sylvester JT, Shimoda LA, Aaronson PA and Ward JPT. Hypoxic pulmonary vasoconstriction. Physiol Rev 92(1):367-520, 2012.

61. Shimoda LA. 55th Bowditch Lecture: Effects of chronic hypoxia on the pulmonary circulation: Role of HIF-1. J Appl Physiol 113(9):1343-52, 2012.

62. Pisarcik, A, Maylor J, Lu W, Undem C, Sylvester JT, Semenza GL, Shimoda LA. Hypoxic induction of hypoxia-inducible factor 1 requires endothelin-1 in pulmonary arterial smooth muscle. Am J Physiol Lung Cell Mol Physiol 304(8):L549-61, 2013.

63. Shimoda LA, Laurie SS. Vascular remodeling in pulmonary hypertension. J Mol Med 91(3):297-309, 2013. 64. Polak J, Shimoda LA, Drager L, Undem C, McHugh H, Polotsky V, Punjabi NM. Intermittent hypoxia impairs

glucose homeostasis in mice: partial improvement with cessation of exposure. Sleep 36(10):1483-90, 2013. 65. Damarla M, Parniani AR, Johnston L, Maredia H, Serebreni L, Hamdan O, Sidhaye VK, Shimoda LA, Myers A,

Crow MT, Schmidt EP, Machamer CE, Gaestel M, Rane MJ, Kolb TM, Kim BS, Damico RL, Hassoun PM. MK2 mediates apoptosis during lung vascular permeability by regulating movement of cleaved caspase 3. Am J Resp Cell Mol Bio 50:932-41, 2014.

66. Lai N, Lade J, Leggett K, Yun X, Baksh S, Chau E, Crow MT, Sidhaye VK, Wang J, Shimoda LA. The aquaporin 1 C-terminal tail is required for migration and growth of pulmonary arterial myocytes. Am J Resp Cell Mol Bio 50(6):1010-20, 2014.

67. Takano N, Peng YJ, Kumar GK, Luo W, Hu H, Shimoda LA, Suematsu M, Prabhakar NR, Semenza GL. Hypoxia-inducible factors regulate human and rat cystathionine ß-synthase gene expression. Biochem J 458:203-11, 2014.

68. Shimoda LA, Laurie SS. HIF and pulmonary responses to hypoxia. J Appl Physiol 116(7):867-74, 2014. 69. Sikka G, Hussmann GP, Pandey D, Cao S, Hori D, Park JT, Steppan J, Kim JH, Barodka V, Myers AC, Santhanam

L, Nyhan D, Halushka MK, Koehler RC, Snyder SH, Shimoda LA, Berkowitz DE. Melanopsin mediates light-dependent relaxation in blood vessels. Proc Natl Acad Sci U S A. 111(50):17977-82, 2014

70. Polak J, Studer-Rabeler K, McHugh H, Hussein M and Shimoda LA. A System for Exposing Cultured Cells to Intermittent Hypoxia Utilizing Gas Permeable Cultureware. Gen Physiol Biophys 34(3):235-47, 2015.

71. Undem C, Luke T and Shimoda, LA. Contribution of elevated intracellular calcium to pulmonary arterial myocyte alkalinization during chronic hypoxia. Pulm Circ 6(1):93-102, 2016.

72. Suresh K, Servinsky L, Reyes J, Baksh S, Undem C, Caterina M, Pearse DB and Shimoda LA. Hydrogen peroxide-

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induced calcium influx in lung microvascular endothelial cells involves TRPV4. Am J Physiol Lung Cell Mol Physiol. 309(12):L1467-77, 2015.

73. Walker J, Undem C,Yun X, Lade J, Jiang H and Shimoda LA. Role of Na+/H+ exchange and Rho kinase in hypoxia-induced pulmonary arterial smooth muscle cell proliferation and migration. Physiol Rep 4(6). pii: e12702, 2016.

74. Huetsch J, Jiang H, Larrain C and Shimoda LA. The Na+/H+ exchanger contributes to increased smooth muscle proliferation and migration in pulmonary arterial hypertension. Physiol Rep 4(5). pii: e12729, 2016.

75. Weiszenstein M, Shimoda LA, Koc M, Seda O, Polak J. Inhibition of Lipolysis Ameliorates Diabetic Phenotype in a Mouse Model of Obstructive Sleep Apnea. Am J Respir Cell Mol Biol. 55(2):299-307, 2016.

76. Suresh K, Servinsky L, Reyes J, Undem C, Zaldumbide J, Rentsendorj O, Dodd-o J, Scott A, Pearse DB and Shimoda LA. CD36 mediates H2O2-induced calcium influx in lung microvascular endothelial cells. Am J Physiol Lung Cell Mol Physiol. 312(1):L143-L153, 2017.

77. Lu H, Chen I, Zhang C, Shimoda LA, Zhang H, and Semenza GL. Chemotherapy-induced GSTO1 Interacts with Ryanodine Receptor RYR1 to Trigger Ca2+-dependent Breast Cancer Stem Cell Enrichment. Cell Rep 18(8):1946-1957, 2017.

78. Yun X, Jiang H, Lai N, Wang J, and Shimoda LA. Aquaporin 1-mediated changes in pulmonary arterial smooth muscle cell migration and proliferation involve β-catenin. Am J Physiol Lung Cell Mol Physiol. 313(5):L889-L898, 2017

79. Barreto Ortiz S, Hori D, Nomura Y, Yun X, Jiang H, Yong H, Chen J, Paek S, Pandey D, Sikka G, Bhatta A, Gillard A, Steppan J, Kim JH, Adachi H, Barodka V, Romer L, An SS, Shimoda LA, Santhanam L, Berkowitz DE. Opsin 3 and 4 mediate light-induced pulmonary vasorelaxation that is potentiated by G-protein receptor kinase 2 inhibition. Am J Physiol Lung Cell Mol Physiol. 314(1):L93-L106, 2018.

80. Suresh K, Servinsky L, Jiang H, Huetsch J and Shimoda LA. Reactive oxygen species induced Ca2+ influx via TRPV4 and microvascular endothelial dysfunction in the SU5416/hypoxia model of pulmonary arterial hypertension. Am J Physiol Lung Cell Mol Physiol, 314(5):L893-L907, 2018.

81. Polak J, Punjabi NM and Shimoda LA. Blockade of endothelin-1 receptor type b ameliorates glucose intolerance and insulin resistance in a mouse model of obstructive sleep apnea. Front Endocrinol 9:280. doi: 10.3389/fendo.2018.00280, 2018.

82. Pandey D, Nomura Y, Rossberg M, Hori D, Bhatta A, Leucker T, Santhanam L, Shimoda L, Berkowitz D, and Romer L. Hypoxia triggers SENP1 modulation of kruppel-like factor 15 and transcriptional regulation of arginase 2 in pulmonary endothelium. Arterioscler Thromb Vasc Biol. 38(4):913-926, 2018.

83. Huetsch JC, Walker J, Undem C, Lade J, Yun X, Baksh S, Jiang H, Lai N, and Shimoda LA. Rho kinase and Na+/H+ exchanger mediate endothelin-1-induced pulmonary arterial smooth muscle cell proliferation and migration. Physiol Reps 6(9):e13698, 2018.

84. Suresh K, Servinsky L, Jiang H, Bigham Z, Zaldumbide J, Huetsch J, Kliment C, Acoba M, Claypool S, Damarla M, Shimoda LA. Mitochondrial ROS and intracellular Ca2+ regulate mitochondrial morphology in microvascular endothelial cells isolated from the Su5416/hypoxia rat model of pulmonary arterial hypertension. Am J Physiol Lung Cell Mol Physiol, 317(5):L639-L652, 2019.

85. Suresh K, Carino K, Johnston L, Servinsky L, Machamer C, Kolb TM, Lam H, Dudek SM, An S, Rane MJ, Shimoda LA and Damarla M. A non-apoptotic, endothelial barrier-protective role for caspase 3. Am J Physiol Lung Cell Mol Physiol, 316(6):L1118-L1126, 2019.

Inventions, Patents, Copyrights

None Extramural Sponsorship Current Grants 2003-2021 “Na+/H+ exchange and pulmonary hypertension” R01 HL73859 National Institutes of Health Total Direct Costs: $1,000,000 PI: Larissa Shimoda (30% effort) 2016-2020 “Targeting divergent roles of caspase 3 to promote endothelial barrier integrity”

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R01 HL133413 National Institutes of Health Total Direct Costs: $1,000,000 Principal Investigator: M Damarla Role: Co- Investigator (10% effort) 2019-2022 “Implications of Obstructive Sleep Apnea for Fat Metabolism” R01 HL146709 National Institutes of Health Total Direct Costs: $1,877,780 Principal Investigator: N. Punjabi Role: Co- Investigator (15% effort) Pending Previous

1993-1994 “Mechanisms of flow-induced responses in piglet cerebral arteries” Pre-doctoral Fellowship 93-F-Pre-16 Wisconsin Heart Association Total Direct Costs: $8,800 Principal Investigator: Larissa Shimoda 1994-1995 “Mechanisms of pulsatile flow responses in piglet cerebral arteries” Pre-doctoral Fellowship 94-Pre-19 Wisconsin Heart Association Total Direct Costs: $8,800 Principal Investigator: Larissa Shimoda 1996 “Role of endothelin in altered vascular reactivity with chronic hypoxia” Postdoctoral Fellowship (declined) American Lung Association Total Direct Costs: $24,246 Principal Investigator: Larissa Shimoda 1996-1998 “Mechanism of pulmonary vasoconstriction by endothelin” National Research Service Award (HL 09543) National Heart, Lung and Blood Institute Total Direct Costs: $23,700 Principal Investigator: Larissa Shimoda

1999-2003 “Chronic hypoxic pulmonary hypertension and endothelin-1: cellular mechanisms in pulmonary vascular

smooth muscle.” Scientist Development Award (993055N) American Heart Association (National)

Total Direct Costs: $260,000 Principal Investigator: Larissa Shimoda

2001-2002 “cGMP and VASP in oxidant endothelial dysfunction” Grant-in-Aid award American Heart Association (National) Total Direct Costs: $195,000 Principal Investigator: DB Pearse

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Role: Collaborating Investigator (5%)

2001-2006 “Hypoxic pulmonary hypertension: contractile mechanisms.” K02 HL67919 National Institutes of Health Total Direct Costs: $ 344,172 Principle Investigator: Larissa Shimoda

1998-2005 “Acute pulmonary arterial responses to hypoxia.” R01HL75113 National Institutes of Health Total Direct Costs: $1,250,000 Principal Investigator: JT Sylvester

Role: Co-Investigator (10%)

2002-2006 “The role of cGMP in oxidant-mediated endothelial injury” R01HL67189 National Institutes of Health Total Costs: $1,125,000 Principal Investigator: DB Pearse

Role: Collaborating Investigator (5%) 2004-2005 “Ca regulation in chronic hypoxic pulmonary hypertension” R01HL75134 National Institutes of Health Total Costs: $1,250,000 Principal Investigator: JSK Sham Role: Collaborating Investigator (5%) 2004-2009 “Calcium signaling in hypoxic pulmonary vasoconstriction” R01 HL75113 National Institutes of Health Total Costs: $1,400,000 Principal Investigator: JT Sylvester Role: Co-Investigator (10%) 2008-2011 “Role of aquaporin-1 in hypoxia-induced pulmonary arterial smooth muscle cell migration” Mid-Atlantic Grant-in-Aid American Heart Association Total Direct Costs: $120,000 Principal Investigator: Larissa Shimoda 2003-2012 “Chronic hypoxia and pulmonary vascular smooth muscle” R01 HL67191 National Institutes of Health Total Direct Costs:$ 1,125,000 Principal Investigator: Larissa Shimoda

2009-2012 “Mechanisms Linking Sleep-Disordered Breathing and Cardiovascular Disease” RC1 HL 099952 (ARRA Challenge Grant) National Institutes of Health

Total Direct Costs: $1,000,000 Principal Investigator: Larissa Shimoda and Naresh Punjabi

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2012 Diversity Supplement (declined) R01 HL73859-S1 National Institutes of Health Total Direct Costs:$ 111,695 Principal Investigator: Larissa Shimoda 2012-2013 “Interrogation of the cellular pathogenesis of pulmonary hypertension” R21 HL096982 National Institutes of Health Total Direct Costs: $300,000 PI: Larissa Shimoda (5% effort) 2010-2015 “Sleep apnea and dysregulation of lipid metabolism” R01 HL80105 National Institutes of Health Total Direct Costs: $1,000,000 PI: VY Polotsky Role: Co-investigator (5% Effort) 2010-2015 “BMP4 in pulmonary vascular smooth muscle” R01 HL93020 National Institutes of Health Total Direct Cost: $1,000,000 Principal Investigator: J Wang Role: Co-investigator (5% Effort) 2012-2016 “Functional linkage of NHE1 and calpain in IPAH” R03 HL114902 National Institutes of Health Total Direct Costs: $100,000 PI: Larissa Shimoda (5% effort) 2014-2019 “AQP1 and Pulmonary Hypertension” R01 HL126514 National Institutes of Health Total Direct Costs: $1,000,000 Principal Investigator: L Shimoda (20% Effort) 2015-2019 “Non-visual opsins & vasoregulation: implications for vascular therapy” R01 HL124213 National Institutes of Health Total Direct Costs: $1,000,000 Principal Investigator: D Berkowitz Role: Co- Investigator (10% effort) Research Program Building / Leadership 2007-2008 Organizer, Pulmonary Division Hypoxia Working Group 2014-present Director of Bench Research, Pulmonary Division EDUCATIONAL ACTIVITIES Educational Publications Book Chapters 1. Sylvester JT, Sham JSK, Shimoda LA, Liu, Q. Cellular mechanisms of acute hypoxic pulmonary vasoconstriction.

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In: Respiratory-Circulatory Interactions in Health and Disease. Ed by M Pinsky and S Scharf, New York, Marcel Dekker, 315-359, 2001.

2. Shimoda LA, Semenza GL. Functional analysis of the role of hypoxia-inducible factor-1 in hypoxic pulmonary hypertension. In: Methods in Enzymology: Oxygen Sensing. Ed by J. N. Abelson and M.I. Simon, New York, Academic Press, Inc., 121-133, 2004.

3. Shimoda LA. Pulmonary Circulation. In: Encyclopedia of Respiratory Medicine. Ed by G. J. Laurent and S. Shapiro, Oxford, England, Elsevier Ltd., 537-544, 2005.

4. Semenza GL, Shimoda LA, Prabhakar NR. Regulation of gene expression by HIF-1. In: Signaling pathways in acute oxygen sensing. Ed by D.J. Chadwick and J. Goode, Chichester, United Kingdom: John Wiley & Sons Ltd., 2-14, 2005.

5. Shimoda LA. Hypoxic regulation of ion channels and transporters in pulmonary vascular smooth muscle. Adv Exp Med Biol. 661:221-35, 2010.

6. Maylor J, Shimoda LA. Intracellular pH, Na+/H+ exchange and pulmonary arterial smooth muscle cell function. In: Recent Advances in Pulmonary Vascular Biology. Ed by YX Wang, Kerala, India, Research Signpost, 15-38, 2012.

7. Yang C, Jiang L, Zhang H, Shimoda LA, DeBerardinis RJ, Semenza GL. Analysis of hypoxia‐induced metabolic reprogramming. Methods in Enzymology: Oxygen Sensing, 542:425-55, 2014.

8. Suresh K and Shimoda LA. Lung Circulation. Comp Physiol 6(2):897-943, 2016. 9. Suresh K and Shimoda LA. Endothelial reactive oxygen species and intracellular calcium signaling in pulmonary

hypertension. Adv Exp Med Biol. 2017; 967:299-314, 2017. 10. Frump A and Shimoda LA. Sex differences in pulmonary arterial hypertension. In: Sex-Based Differences in Lung

Physiology. Ed by P Silveyra and X Tigno. In preparation 11. Huetsch JC and Shimoda LA. Update to Pulmonary Circulation. In: Encyclopedia of Respiratory Medicine. Ed by G.

J. Laurent and S. Shapiro, Oxford, England, Elsevier Ltd., in preparation Invited Reviews 1. Shimoda LA, Sham JSK, Sylvester JT. Altered vasoreactivity in the chronically hypoxic lung. Physiol Res 49:549-560,

2000. 2. Shimoda LA, Sham JSK, Liu Q, Sylvester JT. Acute and chronic hypoxic pulmonary vasoconstriction: a central role

of endothelin-1? Respir Physiol Neurobiol. 132: 93-106, 2002. 3. Raj U, Shimoda LA. Oxygen-dependent signaling in pulmonary vascular smooth muscle. Am J Physiol Lung Cell Mol

Physiol 283: L671-678, 2002. 4. Huestch J, Shimoda LA. Na+/H+ exchange and hypoxic pulmonary hypertension. Pulm Circ 5(2):228-43, 2015. 5. Maron, BA, Marchado, RF and Shimoda, LA. Pulmonary Vascular and Ventricular Dysfunction in the Susceptible

Patient: 2015 Grover Conference Series. Pulm Circ 6(4): 426-438, 2016. 6. Huetsch JC, Suresh K, Bernier M and Shimoda, LA. Update on Novel Targets and Potential Treatment Avenues in

Pulmonary Hypertension. Am J Physiol Lung Cell Mol Physiol 311(5):L811-L831, 2016. 7. Yun X, Sikka G and Shimoda LA. Revisiting hypoxia inducible factors in pulmonary hypertension. Curr Opin Physiol,

in press. 8. Huetsch J, Suresh K and Shimoda LA. NADPH oxidase and smooth muscle cell proliferation in pulmonary

hypertension. Antioxidants, in press 9. Tejwani V, Yun X, Sikka G, Shimoda L, Suresh K. Recommended reading from the johns hopkins university

pulmonary and critical care medicine research fellows. Am J Respir Cell Mol Biol. 2019 Apr 30. doi: 10.1165/rcmb.2019-0075RO

10. Shimoda LA. Cellular basis of pulmonary vascular remodeling by hypoxia. Physiol, submitted 11. Shimoda LA and Semenza GL. Long and winding road: deciphering the role of HIF-1 in the pulmonary vasculature.

Am J Physiol Lung Cell Mol Physiol, submitted Invited Editorials 1. Shimoda LA and Kuebler WK."Hypoxio-spondin": Thrombospondin and its emerging role in pulmonary

hypertension. Cardiovasc Res 109(1):1-3, 2016. 2. Huetsch JC, Suresh K, Shimoda LA. When higher cholesterol is better: membrane cholesterol loss and endothelial

calcium signaling. Am J Physiol Heart Circ Physiol. 314(4):H780-H783, 2108. 3. Shimoda LA. Let’s Talk About Sex: A Novel Mechanism by Which Estrogen Receptor β Limits HIF expression in

Pulmonary Endothelial Cells. Am J Resp Cell Mol Biol, 59(1):11-12, 2108.

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4. Shimoda LA. What's HIF Got to Do with It? HIF-2 Inhibition and Pulmonary Hypertension. Am J Respir Crit Care Med. 198(11):1363-1365, 2108.

5. Shimoda LA. Overview: pulmonary vascular function in health and disease. J Physiol 597(4):973-974, 2019. 6. Shimoda, LA. CA Dreamin': Carbonic anhydrase inhibitors, macrophages and pulmonary hypertension. Am J Resp

Cell Mol Biol, 61(4):412-413, 2019. 7. Shimoda LA. Raise your glass: celebrating the 2019 Nobel Prize in Physiology or Medicine. Am J Physiol Lung Cell Mol

Physiol, in press Teaching Classroom instruction 2014-present Planning and Funding Clinical Research I and II, Course Instructor, School of Public Health 2017-present Hypoxic Pulmonary Vasoconstriction, Lecturer, Department of Physiology, School of Medicine Mentoring (*indicates URM) Advisees- Postdoctoral 1998-2002 *Dominador J. Manalo, PhD (co-mentor)

-awarded American Heart Association (National) Pulmonary Hypertension Fellowship -current position: Staff Scientist, FDA, Rockville, MD

2005-2006 *Joseph Mauban, PhD,

-current position: Research Faculty, University of Maryland

2009-2010 Andrew Stec, MD -current position, Associate Professor and Director of Pediatric Urology, Medical University of South Carolina

2010-2011 Kristina Suson, MD

-current position: Assistant Professor, Children’s Hospital of Michigan 2010-2011 Tom Cherng, PhD

-current position: Physicians Assistant, NorthShore University Healthsystem, Chicago, Il

2011-2012 Eric Massanyi, MD -awarded Bladder Exstrophy Research Foundation grant -current position: Associate Professor, Northeast Ohio Medical University

2010-2013 Jan Polak, MD, PhD (co-mentor)

-awarded American Sleep Medicine Foundation Strategic Research Grant -current position: Associate Professor and Deputy Head 2nd Internal Medicine Department St. Charles University, Prague

2012-2013 Heather DiCarlo, MD

-awarded Bladder Exstrophy Research Foundation grant -current position: Assistant Professor and Director of Bench Research, Brady Urological Institute, Johns Hopkins

2012-2014 Steve Laurie, PhD -current position: Senior Scientist, Wyle, Houston, TX 2012-pres Karoline Moon, MD, Pulmonary and Critical Care Medicine, Johns Hopkins -awarded Best Science Grant, American Sleep Medicine Foundation -current position: Instructor (adjunct), Pulmonary and Critical Care Medicine, Johns Hopkins

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2013-pres Karthik Suresh, MD, Pulmonary and Critical Care Medicine, Johns Hopkins -awarded Individual National Research Service Award (NIH F32) -awarded Best Basic Research Presentation, 2014 Young Investigator’s Forum in Respiratory Disease -awarded Herman Rahn Award, 2016 -awarded Gilead Sciences Research Scholars Program Award -awarded Parker B Francis Fellow -awarded NHLBI K08 grant -awarded, Young Investigator Prize, ATS, 2017 Grover Conference -current position: Assistant Professor, Pulmonary and Critical Care Medicine, Johns Hopkins 2013-pres John Huetsch, MD, Pulmonary and Critical Care Medicine, Johns Hopkins

-awarded Individual National Research Service Award (NIH F32) -awarded NHLBI K08 grant -awarded AHA Fellow-to-Faculty Award grant (declined) -awarded PHA KO8 supplement grant -current position: Assistant Professor, Pulmonary and Critical Care Medicine, Johns Hopkins

2013-2014 Ezekiel Young, MD -current position: Assistant Professor, Department of Pediatric Urology, Stony Brook 2014-2015 Peter Stuhldreher, MD -current position: Pediatric Urologist, US Navy 2015-2016 Timothy Baumgartner, MD

-current position: Pediatric Urologist, US Air Force 2016-2017 Matthew Kasprenski, MD -current position: Pediatric Urologist, US Army 2016-pres Meghan Bernier, MD (co-mentor) -awarded, Robyn Barst Pediatric PH Research Award -awarded, Actelion Research Grant

-awarded, Pearl M. Stetler Award -current position: Instructor, Department of Anesthesiology and Critical Care, Johns Hopkins 2016-pres Xin Yun, MD, PhD

-awarded, American Heart Association Fellowship Grant 2017-2018 Thomas Nicholson, MD (co-mentor) -awarded, Individual National Research Service Award (NIH F32, declined) -current position: clinical fellow, Johns Hopkins 2017-2018 Liron Berger, PhD -current position: Researcher, Technion, Israel 2018-pres Gautam Sikka, MD 2018-pres *Stanley Chen-Cardenas, MD 2019-pres Jochen Steppan, MD (co-mentor) -current position: Assistant Professor, Department of Anesthesiology - awarded NHLBI K08 grant 2019-pres Ryan Belacanech, MD

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2019-pres Wayland Wu, MD

Advisees Pre-doctoral 2010-2013 Ning Lai, MD, MS

-awarded a pre-doctoral fellowship grant from the China Scholarship Council -awarded PhD 7/2013 from Guangzhou Medical University -current position: Faculty, Guangzhou Medical University

2012-2016 Xin Yu, MD

-awarded a pre-doctoral fellowship grant from the Overseas Study Project of Guangzhou -awarded PhD 7/2016 from Guangzhou Medical University -awarded APS Respiration Section poster presentation prize, EB 2016 -current position: postdoctoral fellow, Pulmonary and Critical Care Medicine, Johns Hopkins

Undergraduate and post-baccalaureate 6/2001-8/2001 *Eon J. Rios, Summer Minority Internship Program

- awarded Best Student Poster Presentation -Graduate: UC-Davis - Graduate: MD/PhD, Stanford Medical School -current position: Dermatology Fellow, Stanford

8/2000-7/2007 *Letitia Weigand, BS

-Graduate: PhD, Johns Hopkins University -current position: Health Program Specialist, Office of Training Career Development and Workforce Diversity, NIH

6/2002-8/2002 *Joe Plum, Summer Minority Internship Program

-Graduate-Massachusetts Institute of Technology -current position: Teacher, Gaston Preparatory School

6/2003-8/2003 Elizabeth Miles Whitman,

- Graduate-Yale University - Graduate-MD, University of Virginia -current position: Emergency Medicine Faculty, Georgetown

6/2003-8/2003 *Tasha Kawamata, Summer Minority Internship Program

- Graduate-University of Hawaii - awarded Best Poster Presentation- Health and Medicine Category, National Meeting of the Society for the Advancement of Chicanos and Native Americans in Science - current position: Outreach Specialist, Career and Technical Education Center, University of Hawaii

6/2004-8/2012 Clark Undem,

- Graduate-University of Maryland, Baltimore County - Graduate-MS/MBA, Johns Hopkins University -current position: Associate Biologics Project Manager, MedImmune

6/2004-8/2004 *Trevor Luke, Summer Minority Internship Program 6/2005- 8/2007 Full-time

-Graduate- New Mexico State University - Finalist, David Bruce Award for Excellence in Undergraduate Research, American Physiologic Society, Experimental Biology 2005

- Graduate: PharmD, UCSF

13

- current position: Pharmacist, University California at Davis

6/2005-8/2005 Timothy Schaffner - Graduate- St. Francis University - Graduate- MD, West Virginia University - current position: Surgical Resident, University at Buffalo SOM

6/2007-8/2007 *Jasmine Walker, Summer Minority Internship Program 6/2008-8/2008 - Graduate- Yale University

- Graduate- MD, Columbia -current position: Surgical Residency, Vanderbilt

6/2009-8/2009 *Edsel Abud, Summer Minority Internship Program 6/2010-8/2010 -Graduate: California State Univ-Long Beach

-current position: MD/PhD Student, UC-Irvine

6/2009-8/2009 *Antoinette Dawson, Summer Minority Internship Program -Graduate: Tougaloo College -Graduate: MD, Brown -current position: Psychiatry Resident, US Army

6/2010-8/2010 *Kyle Leggett, Summer Minority Internship Program

- Graduate: Wright State University -Graduate: MD, Wright State University - current position: ED Physician, Canton, OH - Winner, David Bruce Abstract Award for Excellence in Undergraduate Research, American Physiologic Society, Experimental Biology 2011 - Winner, David Bruce Presentation Award for Excellence in Undergraduate Research, American Physiologic Society, Experimental Biology 2011

6/2011-8/2011 *Chelsea Earby, Summer Minority Internship Program

-Graduate: Florida A & M -current position: MD Student, Ohio State University -Winner, Best Poster Presentation, Peach State Research Conference

6/2011-8/2012 *Janice Bonsu -Graduate: Johns Hopkins University

- Graduate: MPH, University of Pennsylvania -current position: MD student, Ohio State University

6/2011-8/2011 Arjun Punjabi 6/2012-8/2012 -Graduate: Brown University

- current position: PhD Student, Northwestern University 6/2013-8/2013 *Max Korzec, Summer Minority Internship Program

-Graduate: Northern Illinois University - current position: MD Student, Southern Illinois School of Medicine

6/2013-8/2013 Stephan Maman, Summer Minority Internship Program 5/2014-8/2014 -Graduate: Southern Utah State University

- current position: MD Student, Penn State School of Medicine 6/2015-8/2015 *Carolina Larrain, Summer Minority Internship Program -Graduate: University of Wisconsin

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-current position: MD Student, University of Wisconsin 6/2015-8/2015 *Jose Reyes, Summer Minority Internship Program -Graduate: Texas State University

-current position, MD student, Johns Hopkins School of Medicine 6/2016-8/2016 *Joel Zalumbide, Summer Minority Internship Program (co-mentor) 7/2017-12/2017 -Graduate, Johns Hopkins University -current position: MD Student, Emory University 6/2016-8/2016 *Larissa Breden, Summer Minority Internship Program (co-mentor)

-Graduate: University of New Mexico -current position, MD Student, Johns Hopkins School of Medicine

5/2017-7/2017 *Zahna Bigham, Summer Minority Internship Program (co-mentor) -Graduate: Wesleyan College

-current position: MD/PhD Student, Tufts School of Medicine 5/2017-7/2017 *Micheal Munson, Summer Minority Internship Program 5/2018-7/2018 -current position, Senior, Baylor University 5/2019-7/2019 -awarded, Best Poster Presentation, ABRCMS -awarded, Travel Award, ASCB Advisees- High School 6/2010-8/2010 Arjun Punjabi -Graduate: BS, Brown University

- current position: PhD Student, Northwestern University

6/2018-pres *Zion Smith -awarded Johns Hopkins CARES Scholarship - Graduate: Baltimore City College

- current position: Freshman, USC

K08/AHA Advisory Committees 2008-2013 Venketaramana Sidhaye, MD: “Effects of shear stress on regulation of epithelial permeability.” 2009-2014 Mahendra Damarla, MD: “MAP kinase signaling in apoptosis-induced ventilator-associated lung injury.” 2013-2018 Todd Kolb, MD PhD: “Regulation of Pulmonary Hypertension Induced RV Angiogenesis by MIF” 2019-2023 Jochen Steppan, MD: “The therapeutic role for LOXL2 in pulmonary hypertension” Training Grant Participation 2001-present Program Faculty, T32 Multidisciplinary training program in lung diseases 2001-present Program Faculty, T35/R25 Short term training for minority students Educational Program Building/Leadership 2006-2009 T32 Fellows Journal Club Conference Director 2006-2017 T32 Fellows Research-in-Progress Conference Director 2004-2008 Program Co-Director, T35/R25 Short Term Training Program to Increase Diversity in Health Related

Research 2009-present Program Director, R25 Short Term Training Program to Increase Diversity in Health Related Research 2016-present Program Co-Director, T32 Multidisciplinary Training Program in Lung Diseases Educational Extramural Funding Current: 2012-2023 “Short Term Training Program to Increase Diversity in Health Related Research”

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R25 HL084762 National Institutes of Health Principle Investigator: Larissa Shimoda and Mahendra Damarla 2016-2023 “Multidisciplinary training program in lung diseases” T32 HL007534 National Institutes of Health Principle Investigator: Robert Wise and Larissa Shimoda Pending: None Previous: 2006-2012 “Short Term Training Program to Increase Diversity in Health Related Research” T35/R25 HL084762 National Institutes of Health Principle Investigator: LS King Role: Co-PI CLINICAL ACTIVITIES N/A SYSTEM INNOVATION AND QUALITY IMPROVEMENT ACTIVITIES N/A ORGANIZATIONAL ACTIVITIES Institutional Administrative Appointments 2009-present Member, Pulmonary Division Fellows Research Oversight Committee 2011-2014 Member, School of Medicine Women’s Task Force 2014-present Director for Bench Research, Pulmonary Division 2015-present Member, Bayview Scientific Advisory Committee 2015-present Member, Dept. of Medicine Research Council Editorial Activities Editorial Board Appointments 2006-pres Editorial Board Member, American Journal of Physiology: Lung Cellular and Molecular Physiology 2010-pres Editorial Board Member, Pulmonary Circulation 2012-pres Editorial Board Member, Journal of Applied Physiology 2013-pres Editorial Board Member, Physiological Reports 2013-2017 Associate Editor, Physiological Reports 2015-pres Associate Editor, American Journal of Physiology: Lung Cellular and Molecular Physiology 2019-pres Reviewing Editor, The Journal of Physiology 2019-pres Deputy Editor, American Journal of Physiology: Lung Cellular and Molecular Physiology

Guest Editor 2018 Special Issue: Pulmonary Vascular Function in Health and Disease, Journal of Physiology Journal Reviewer 1999-present

Journal of Applied Physiology American Journal of Respiratory Cell and Molecular Biology American Journal of Physiology: Heart and Circulatory Physiology American Journal of Physiology: Cell Physiology

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American Journal of Physiology: Lung Cellular and Molecular Physiology American Journal of Physiology: Gastrointestinal and Liver Physiology Physiological Genomics British Journal of Pharmacology Microvascular Research Neuropeptides Life Sciences American Journal of Respiratory and Critical Care Med American Journal of Respiratory Cell and Molecular Biology Free Radical Research Journal of Experimental Zoology Part A: Ecological Genetics and Physiology Avian Pathology High Altitude Medicine and Biology Circulation Research Circulation Experimental Physiology Neonatology PNAS Journal of Molecular Medicine Nature Chemical Biology PLos One Journal of Physiology Pediatric Research Journal of Clinical Investigation European Respiratory Journal

Advisory Committees, Review Groups/Study Sections Local 1999-2003 Member, Research Grant Review Committee -American Heart Association (Mid-

Atlantic Region) 2017-pres Member, Henrietta Lacks Scholarship Selection Committee, Johns Hopkins University National 2004 Invited Temporary Reviewer; Lung Injury, Repair and Remodeling Study Section; National Heart Lung

and Blood Institute; National institutes of Health 2004 Reviewer, Special Emphasis Panel ZRG1 RES-B 02; NHLBI; National Institutes of Health 2004-2007 Reviewer, Phillip Morris External Research Program 2005-2009 Member, Molecular Signaling 1 Peer Review Study Group -American Heart Association (National) 2006 Reviewer, Special Emphasis Panel ZRG1 RES-B 03; NHLBI; National Institutes of Health; 2009 Reviewer, Special Emphasis Panel/Scientific Review Group 2009 10 HLBP (Program Project Review);

NHLBI; National Institutes of Health 2009-2010 Member, Lung, Respiration and Resuscitation Peer Review Study Group -American Heart Association

(National) 2009-2011 Member, American Heart Association Critical Care, Cardiopulmonary and Resuscitation Council

Cournand and Comroe Award Review Committee 2010 Member, NHLBI Special Emphasis Panel/Scientific Review Group PPG Workgroup 030 2010 Member, NHLBI Special Emphasis Panel/Scientific Review Group 2010/05 HLBP 2010-2011 Invited Temporary Reviewer; Respiratory, Integrative and Translational Biology Study Section; National

Heart Lung and Blood Institute; National institutes of Health 2010 Reviewer, National Science Foundation CAREER Grants 2010, 2011 Member, NHLBI Special Review Group/PPG Workgroup 2011 (Feb) Reviewer, Special Emphasis Panel (RIBT and LIRR Conflicts); NHLBI; National Institutes of Health 2011 (Oct) Reviewer, Special Emphasis Panel (RIBT and LIRR Conflicts); NHLBI; National Institutes of Health 2012 Member, Special Emphasis Panel ZHL1 CSR-H (M1), NHLBI; National Institutes of Health

17

2012-2016 Regular Member, Respiratory, Integrative and Translational Biology Study Section; National Heart Lung and Blood Institute; National institutes of Health

2012, 2013 Judge, NIDDK STEP-UP Program 2014 Reviewer, VA Merit Reviews, PULM MED Group 2015 Reviewer, NHLBI Special Review Group/PPG Workgroup 2016 (May) Reviewer, NHLBI Special Review Group/PPG Workgroup 2016 Reviewer, NHLBI Special Emphasis Panel (ZRG1 VH-N (03)) 2016 (Sept) Reviewer, NHLBI Special Review Group/PPG Workgroup 2016 Reviewer, AHA Lung and Resuscitation Clinical Peer Review Study Group 2016 (Dec) Chair, Special Emphasis Panel 2017/01 ZRG1 CVRS-L (02) M 2017 Reviewer, NHLBI R35 Study Section 2017 Reviewer, NHLBI Special Review Group/PPG Workgroup 2018 (Feb) Reviewer, NHLBI Special Review Group/PPG Workgroup 2018 Reviewer, NHLBI Fellowships Study Section 2018 (Jun) Reviewer, NHLBI R25 Study Section 2018 Chair, NHLBI R15 Study Section 2018 (Sept) Reviewer, NHLBI Special Review Group/PPG Workgroup 2018 Member, NHLBI Working Group to Investigate Short-Term Research Training Programs in Heart,

Lung, Blood, and Sleep Disorders for Clinicians and PhDs 2018-pres Member, T32 External Advisory Committee, Pulmonary and Critical Care Medicine Division, Brigham

and Women’s Hospital 2018 (Dec) Reviewer, NHLBI R25 Study Section 2019 (Apr) Reviewer, NHLBI Special Emphasis Panel (CVRS-H 03 M) 2019 (Sep) Reviewer, NHLBI Special Emphasis Panel (CVRS-H 03 M) 2019-2022 Member, NHLBI Institutional Training Mechanism (T32) Review Committee 2019-pres Member, T32 External Advisory Committee, Respiratory Research Training Program, Brown University

International 2002 Reviewer; Research Funding and Policy Division; Health Research Board, Ireland 2002 Reviewer, Wellcome Trust 2007 Reviewer; Research Funding and Policy Division; Health Research Board, Ireland 2010 Reviewer; Research Funding and Policy Division; Health Research Board, Ireland 2010 Reviewer, Wellcome Trust 2010 Reviewer, Dunhill Medical Trust 2011 Reviewer, UK Biological and Biosciences Research Council 2012, 2013 Reviewer, French National Research Agency 2012 Reviewer, Czech Science Foundation GACR 2013 Reviewer, National Research Council Canada 2016 Reviewer, British Heart Foundation 2019 Reviewer, Medical Research Council, UK Professional Societies 1995-1999 Member, Biomedical Engineering Society 1995 Member, Sigma Xi 1999-present Member, American Physiologic Society 2000-2004 Member, American Thoracic Society Pulmonary Circulation Long Range Planning Committee 2006-2010 Member-at-Large, Leadership Committee, Critical Care, Cardiopulmonary and Resuscitation Council,

American Heart Association 2006-2010 Member, American Heart Association 2006-present Member, American Thoracic Society 2007-present Member, American Thoracic Society Pulmonary Circulation Planning Committee 2007-2010 Member, American Thoracic Society Pulmonary Circulation Nominating Committee 2007-2010 Respiration Section Representative, American Physiologic Society Committee on Committees 2007-present Member, Respiration Section Steering Committee, American Physiologic Society

18

2009-present Member, American Thoracic Society Pulmonary Circulation Assembly Executive Committee 2009 Member, New York Academy of Sciences 2011-2014 Secretary/Treasurer, Respiration Section, American Physiologic Society 2010 Chair, Nominating Committee, PC Assembly, American Thoracic Society 2013-2015 Chair, Planning Committee, PC Assembly, American Thoracic Society 2014-2017 Chair, Respiration Section, American Physiological Society 2014-present Member, Program Committee, Respiration Section, American Physiological Society 2014-2017 Member, Section Advisory Committee, American Physiological Society 2014-2017 Member, Nominating Committee, American Physiological Society 2017-present Member, Awards Committee, Respiration Section, American Physiological Society 2018 Member, Porter Physiology Development Committee, American Physiological Society 2018-pres Councilor, American Physiological Society 2018-pres Council Liaison, Porter Physiology Development Committee, American Physiological Society Conference Organizer, Session Chair 2002 Symposium titled: “Oxygen dependent signaling in pulmonary vascular smooth muscle”; Experimental

Biology; American Physiological Society Respiration Section; New Orleans, LA Role: Symposium Co-Chairperson

2006 Session titled: “Hypoxic regulation of pulmonary vascular contractility and remodeling”; American Thoracic Society International Conference; San Diego, CA Role: Co-Chairperson

2007 Session titled: “Hypoxic regulation of pulmonary vasomotor tone and remodeling”; American Thoracic Society International Conference; San Francisco, CA Role: Chairperson

2008 Session titled: “Membrane Receptors, Channels and Transporters in Pulmonary Vascular Disease”; American Thoracic Society International Conference; Toronto, Canada Role: Chairperson

2008 Grover Conference on Pulmonary Pathobiology; Decker, CO Member, Program Committee 2008 Session titled: “Structure, Function, and Modulation of Membrane Channels, Transporters, and Receptors in

the Pulmonary Vasculature”; Grover Conference on Pulmonary Pathobiology; Decker, CO Role: Co-Chairperson

2009 Session titled: “Pulmonary Vasoreactivity”; American Thoracic Society International Conference; San Diego, CA Role: Chairperson

2009 Symposium titled: “Cellular Recruitment in Lung Vascular Remodeling and Pulmonary Hypertension;” Experimental Biology; New Orleans, LA Role: Co-Chairperson

2010 Symposium titled: “Pulmonary arterial hypertension: Mechanisms and mediators;” Experimental Biology; Anaheim, CA Role: Co-Chairperson

2009 Member, American Heart Association Critical Care, Cardiopulmonary and Resuscitation Council Fall Program Committee, Orlando, FL

2011 Grover Conference on Pulmonary Pathobiology; Decker, CO Member, Program Committee 2011 Session titled: “Hypoxia and Pulmonary Vascular Cell Signaling;” American Thoracic Society International

Conference; Denver, CO Role: Co- Chairperson 2012 Session titled: “Tissue injury, repair and inflammation;” Keystone Symposium Advances in Hypoxic Signaling;

Banff, Canada Role: Chair 2012 Session titled: “Pulmonary vascular signaling in pulmonary hypertension;” American Thoracic Society

International Conference; San Francisco, CA Role: Co-Chair 2015 Grover Conference on Pulmonary Pathobiology; Decker, CO Member, Program Committee 2016 Session titled: “Hypoxia and the pulmonary vasculature;” American Thoracic Society International

Conference; San Francisco, CA Role: Co-Chair 2017 Session titled: “Organelle Dysfunction in Pulmonary Vascular Disease: Mitochondria, Endoplasmic

Reticulum, Lysosomes and Beyond”; Experimental Biology Conference; Chicago, IL Role: Chairperson 2018 Session titled: “Ballroom (PH) Blitz: Basic Biology”; American Thoracic Society International Conference;

San Diego, CA Role: Co-Chair 2020 Session titled: “Pulmonary Vascular Endothelial Control of Cardio-Pulmonary Diseases: Molecular

Opportunities for Novel Treatments”; Experimental Biology Conference; San Diego, CA Role: Co-Chair Consultantships

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None RECOGNITION Awards/Honors 1986-1987 Marquette University Academic Scholarship 1990-1991 Marquette University Women’s Club Fellowship 1990-1995 Maurice L. Madden Fellowship; Marquette University 1990-1995 Marquette University Graduate School Tuition Scholarship 1995 Sigma Xi Membership 2001 Giles F. Filley Memorial Award for Excellence in Respiratory Physiology and Medicine; American

Physiological Society 2008 New Investigator Award, Mount Desert Island Biological Institute 2008 Young Investigator Award, American Thoracic Society 2011 Bowditch Lecture Awardee, American Physiological Society 2012 Star Reviewer, American Journal of Physiology, Lung Cellular and Molecular Physiology 2017 David M Levine Excellence in Mentoring Award 2019 Estelle Grover Prize Lecture, 2019 Grover Conference, American Thoracic Society Invited Talks/Panels (External) July 1999 “Chronic hypoxia alters Ca2+-dependence of endothelin-1-induced contraction in rat intrapulmonary arterial

smooth muscle”; Symposium titled: “Hypoxic pulmonary hypertension;” Pulmonary Circulation VII; European Respiratory Society

Dec 1999 “Cellular Mechanisms involved in the pathogenesis of chronic hypoxic pulmonary hypertension;” Department of Cell Biology and Physiology, University of New Mexico School of Medicine, Albuquerque, NM.

Apr 2001 “Role of hypoxia-inducible factor-1 in cellular responses to hypoxia;” Symposium titled: “Molecular responses to hypoxia;” Experimental Biology; US Army Research Institute of Environmental Medicine, Orlando, FL.

Apr 2002 “Effect of hypoxia on ion homeostasis in pulmonary vascular smooth muscle;” Symposium titled: “Oxygen dependent signaling in pulmonary vascular smooth muscle”; Experimental Biology 2002; American Physiological Society Respiration Section, New Orleans, LA.

May 2002 “Altered smooth muscle cell ion homeostasis and hypoxic pulmonary hypertension;” Symposium titled; Special topics in pulmonary physiology: the next generation; American Thoracic Society International Conference 2002; Assembly on Pulmonary Circulation, Atlanta, GA.

Dec 2003 “Hypoxia-induced alterations in smooth muscle cell ion homeostasis;” Department of Pharmacology, University of South Alabama, Mobile, AL.

Mar 2004 “Hypoxia-induced alterations in smooth muscle cell ion homeostasis;” Keystone Symposia: The biology of hypoxia: the role of oxygen sensing in development, normal function and disease. Steamboat Springs, CO.

Apr 2004 “Chronic hypoxia and pulmonary vascular smooth muscle: role of hypoxia inducible factor-1;” Symposium titled: The pulmonary vasculature in lung disease; Pulmonary Research Group, Baltimore, MD.

May 2004 “Hypoxic pulmonary hypertension: smooth muscle mechanisms and mediators;” Department of Physiology and Biophysics; Case Western Reserve University, Cleveland, OH.

Feb 2007 “HIF-1 and pulmonary vascular responses to hypoxia;” Center for Perinatal Research; Ohio State University, Columbus, OH.

Jan 2008 “Hypoxic pulmonary hypertension;” Keystone Symposia: Molecular, Cellular, Physiological, and Pathogenic Responses to Hypoxia, Vancouver, BC., Canada

Apr 2008 “Pulmonary arterial smooth muscle responses to chronic hypoxia: role of HIF-1;” Department of Pharmacology, Georgetown University, Washington, DC.

Sept 2008 “Hypoxic regulation of ion channel and membrane transporter expression and function;” Grover Conference on Pulmonary Pathobiology; Denver, CO.

Oct 2008 “Pulmonary arterial smooth muscle responses to chronic hypoxia: role of HIF-1;” Oxygen Club of Greater Washington, National Institutes of Health, Washington, DC.

Mar 2009 “ET-1 induces HIF-1α in pulmonary arterial smooth muscle cells via alterations in HIF-1α and PHD2 expression;” New York Academy of Sciences Conference: Hypoxia and Consequences: From Molecule to Malady;

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New York, NY. Nov 2009 “HIF-1 and pulmonary responses to chronic hypoxia”. Guangzhou Medical University, Guangzhou, China. Nov 2009 “HIF-1 and pulmonary responses to chronic hypoxia”; First Affiliated Hospital of Guangzhou Medical

University and State Key Laboratory for Respiratory Diseases, Guangzhou, China. Jan 2010 “Hypoxic regulation of HIF-1 in the lung;” Keystone Symposia: Molecular Mechanisms of Oxygen Sensing and

Response Pathways, Keystone, CO. Apr 2010 “HIF-1 and pulmonary responses to hypoxia;” Symposium titled: Pulmonary hypertension: mechanisms and

mediators. Experimental Biology 2010; American Physiological Society, Anaheim, CA. Apr 2011 “Effects of chronic hypoxia on the pulmonary circulation: role of HIF-1;” Bowditch Awardee Lecture,

Experimental Biology 2011, American Physiological Society, Washington, DC May 2011 “The chronically hypoxic lung and HIF: a Highly Involved Factor;” Department of Pharmacology and

Toxicology, Medical College of Georgia, Augusta, GA. Jun 2011 “HIF and the pulmonary vasculature;” Annual Retreat of the University of Giessen Lung Center, Giessen,

Germany. Dec 2011 “HIF and pulmonary vascular responses to hypoxia-update;” Guangzhou Medical University, Guangzhou,

China. Feb 2012 “Pulmonary vascular smooth muscle and Na+/H+ exchange: more than meets the ion?” Department of

Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN. May 2012 “Chalk Talk: Pulmonary vascular responses to chronic hypoxia,” Department of Physiology and Biomedical

Engineering, Mayo Clinic, Rochester, MN. Nov 2012 “Na+/H+ exchange and pulmonary vascular smooth muscle function: more than meets the ion?” Center for

Cardiovascular Sciences, Albany Medical Center, Albany, NY. Nov 2012 “Na+/H+ exchange and pulmonary vascular smooth muscle function: more than meets the ion?” Division of

Pulmonary, Allergy, Critical Care, Occupational and Sleep Medicine, Indiana University, Indianapolis, IN. Feb 2013 “HIF inhibitors in rodent models of hypoxic pulmonary hypertension;” 18th International Hypoxia

Symposium, Lake Louise, Alberta, Canada. Mar 2013 “HIF inhibitors and pulmonary hypertension: new tricks for old drugs;” Department of Pediatrics,

Nationwide Children’s Hospital, Columbus, OH. Nov 2013 “Pulmonary vascular function during chronic hypoxia: role of Na+/H+ exchanger;” Department of

Physiology, University of New Mexico School of Medicine, Albuquerque, NM Jan 2014 “Oxygen Sensing and HIF regulation;” Joint Symposium of ECCPS and Pulmonary Vascular Research

Institute; Bad Nauheim, Germany Mar 2014 “Na+/H+ Exchange and Control of Pulmonary Vascular Myocyte Function During Hypoxia,” College of

Medicine Molecular Pharmacology & Physiology, University of South Florida Mar 2015 “Aquaporin 1 and pulmonary hypertension: new roles for old holes”; Department of Pharmacology,

University of Illinois at Chicago, Chicago, IL April 2015 “Aquaporin 1 and pulmonary hypertension: new roles for old holes”; Department of Physiology, College of

Medicine, University of Tennessee Health Science Center, Memphis, TN Sept 2015 “Genomic and functional consequences of chronic hypoxia”, Grover Conference on Pulmonary

Pathobiology; Denver, CO April 2016 “Role of HIF in Hypoxic Pulmonary Hypertension”, Graduate Research Seminar, Basic Sciences

Department, Loma Linda University, Loma Linda, CA April 2016 “Aquaporin 1 and Pulmonary Hypertension: More Than Just a Water Channel”, Center for Perinatal

Research, Loma Linda University, Loma Linda, CA April 2016 “New Pieces of the Pulmonary Hypertension Puzzle: Role of Aquaporins”, Grand Rounds, Pulmonary,

Allergy, and Critical Care Division, University of Pennsylvania, Philadelphia, PA Sept 2016 “Role of HIF-1 in hypoxia-induced pulmonary hypertension” International Conference of Physiological

Sciences, Beijing, China. Oct 2016 “Non-canonical roles for ion channels in pulmonary hypertension”; Department of Pathobiology, Lerner

Research Institute, Cleveland, OH Jan 2017 “Non-canonical roles of channels in pulmonary hypertension”; Division of Pulmonary and Critical Care

Medicine, University of Pittsburgh, Pittsburgh, PA Feb 2019 “Aquaporin 1 and pulmonary hypertension: a (non)watery tale”; Division of Pulmonary and Critical Care

Medicine, Emory University, Atlanta, GA

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Jun 2019 “Hypoxic signaling in pulmonary hypertension”; Aspen Lung Conference, Aspen, CO Jun 2019 “Phototherapy for relieving hypoxic pulmonary hypertension”; 5th International ARDS Conference, Berlin,

Germany Sept 2019 “HIFs in pulmonary hypertension”; Estelle Grover Lecture, Grover Conference on Pulmonary

Pathobiology; Denver, CO