Darshan khatri DRUG RESISTANCE IN ANTIBIOTICS.docx

8/15/2019 Darshan khatri DRUG RESISTANCE IN ANTIBIOTICS.docx

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Bhagwati International PublicSchool

Dr. Indu Dayal Meshri PrimaryEnglish School

INVESTEGT!"# P"!$E%T !N

ST&D# !' D"&GS "ESITN%E INB%TE"I

Guided by() Ms.Seema Tiwari

Submitted by() Darshan *hatri


2/22

"oll no()

This is to certify that Mr. Darshan Khatristudent of XII science Roll

No___________ worked on project titled-ST&D# !'

D"&GS "ESITN%E IN B%TE"I! heldin "ha#wati

International $u%lic &chool durin# theacade'icyear ()*+-()*,.e worked

sincerely under the #uidance of faculties

and prepared this dissertation.

/ternal Teacher &u%ject Teacher

$rincipal


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%*N!+,EDGEMENT

First of all,I am immensely indebted to almighty god for his

blessings and grace without which I could not have undertaken

this task and my efforts would never have been a success. I humbly consider a privilege and honor to

express my heartiest and profound gratitude to Mr. Rajendra..

Malwal, principal !I", "atan. For his appropriate direction,

valuable suggestion, under judging assistance so generously

extended to me.

I wish to express my deepest feelings of gratitude toMs.eema #iwari, biology department, !I" "atan. For his

erudite involvement and sustained guidance which has been

pivotal in my project work. $is minute observation, precious

insights, critical comments have indeed greatly helped to shape

my ideas.

#his guidance and support received from my entireclassmates who contributed and whoare contributing to this

project, is vital for the success of this project. I am grateful

for their constant support and help.


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I also owe sense of gratitude to my parents for

encouragement and support throughout the project.

- Darshan khatri

%ontents

S".N! TIT,E PGE

N!1) Introduction2) History of antibiotics

3) What is drug

resistance?

4) Why do bacteria

become resistant to

antibiotics?5) Aim of project

) !ateria"s

#) $rocedure

%) !ethod

&) 'bser(ation


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1) *esu"t

11) $recautions

12) !essage

Introduction

ntibiotics are drugs that are used

in the treatment or -reention o/ bacterial in/ection. Strictly

s-ea0ing 1antibiotics are naturalsubstance -roduced by

microorganisms to 0ill otherorganisms or retard their growth.

Penicillin1 Tetracycline1Stre-tomycin are /ew drugs used to

treat arious diseases.


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2IST!"# !' NTIBI!TI%S

N%IENT

In 3455 B%1the Sumerian doctors would gie their -atients1beer sou-

with sna0es0in and turtle shells.

Modern 2istory,ouis Pastures was the 6rst to recogni7ethat bacteria could be used to 0ill otherbacteria.In 89:9 Sir le;ander 'leming


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discoered Penicillum Notatum.It ise


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+hy do bacteria become

resistant to antibiotics=ntibiotics resistance is a natrual-henomenon.+hen an antibiotics is used1 bacteriathat can resist that antibiotics hae a greaterchance o/ surial than those that are@Susce-tible?.

Susce-tible bacteria are 0illed or intibited by anantibiotics1 resulting in a selectie -ressure /or thesurial o/ resistant strains o/ bacteria.


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In some countries and oer Internet1 antibiotics canbe -urchased without a doctorAs-rescri-tion.Patients sometimes ta0e antibiotics

unnecessarily1 to treat iral illness li0e commoncold.

im o/ the

-roect


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In this -roect

the aim is toinestigate the

Study o/ deelo-ment o/

drug resistancein bacteria using

antibiotics.


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MaterialsB&NSEN B&"NE" '!"%EPS

S!PP"'I,M


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MT%2ESB"!T2%&,T&"ES !'

S.EPIDE"MIDIS

TS P,TES BENT

G,SS "!D

'INE TIPPED M"*ING NTIBI!TI%S


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8C -ure strain o/ bacteria is isolated/rom an in/ected -erson.

:C This -ure strain is then s-read oerthe sur/ace o/ a s-ecial medium1called Mueller)2iston gar 1to createa lawn 1or car-et o/ bacteria.

3C Small 6lter -a-er discs 1im-regnatedwith standardised amounts o/

antibiotics 1are gently -ressed on tothe sur/ace o/ the agar.

C The -lates are then incubatedoernight 1the antibiotics di


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:C Swirl the contents o/ the brothcultureo/ S.E-idermis until it is eually

mur0y throughtout.&se the sterilecotton swab and glass rod to createa lawn 1or car-et o/ S.e-idermis on :

o/ the -lates o/ TS.

DonAt /orget to hold the ca- in your -in0yand to Hame the mouth o/ the test tubebe/ore and a/ter di--ing the sterile swab

into it.

3C ,abel the bottom o/ -etric dish withyour name 1the date be sure to write

small and only around the edges o/ thebottom -etri dish.

C &se the second sterile swab to re-eatste- two 1e;-ect substitute E.coli /or

S.e-idermis. lso 1label this E.%oil dish.


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4C Select two di


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9C /ter : hours in the incubator 1chec0

/or the -resence o/ antibiotics actiity.

!bserations

,oo0 /or a cleararea 1called L!NE

!' IN2IBITI!Nsurrounding a -a-erdisc.


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Measure the diameter 1in millimeters o/ the 7one o/ inhibition /or each o/ the

di


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determine

whether eachbacteria is

Susce-tibleSensitieC 1

&ne


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between

IntermediateC.

P"E%&TI!NS

microbiology lab is -otentially a erydangerous -lace.'or this reason it is

e;termely im-ortant that you /ollowsa/ety guidelines and always -ractice

sterile techniue when handling microbes1unless instructed otherwise.

8C There should be no boo0s or -a-er atyour wor0station e;ce-t this lab -ac0et.


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:C Neer hae any /ood or drin0s at yourwor0station.

3C Neer o-en a -etri dish a/ter you haeinoculated it and hae allowed it to

incubated oernight.

C lways di-ose o/ used material in thebioha7er bag unless instructed otherwise.

BIBI!,G"P2#

Darshan khatri DRUG RESISTANCE IN ANTIBIOTICS.docx

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