Colleen Worne

University of UtahDepartment of PathologySalt Lake City, UT 84112

colleen.worne@path.utah.edu

Murray, UT 84107(949)293-6623

cworne@gmail.com

Work Experience2015-present University of Utah-School of Medicine

Senior Lab SpecialistSalt Lake City, UT

2013-2015 University of Utah-School of MedicineLab SpecialistSalt Lake City, UT

2012-2013 UC Regents-University of California, IrvineStaff Research Associate IIrvine, CA

2011-2012 University of California, IrvineCIRM Bridges InternIrvine, CA

Education 2011-2012 California State University, Long Beach

Biotechnology Certificate with Emphasis in Stem Cell Biology Long Beach, CA

2008-2011 California State University, Long BeachGeneral Biology, B.S.Long Beach, CA

2005-2008 Saddleback CollegeMission Viejo, CA

Research Experience2013-present Lab Specialist/Manager, Department of Pathology, University of Utah-

Thomas Lane, Ph.D.Studied 1) the functional contributions of chemokines and chemokine receptors in defense and disease following viral infection of the central nervous system (CNS) and 2) evaluated the therapeutic potential of mouse/human neural precursor cells (NPCs) in clinical recovery and remyelination in a model of viral-induced demyelinationTechniques: Lab management*, hESC and DBT culture, intracranial (i.c.) injections, plaque assay, cryostat sectioning, EM tissue preparation, MOG-EAE induction, animal husbandry*See Leadership section

2012-2013 Staff Research Associate I, Department of Anatomy and Neurobiology, University of California, Irvine-Aileen Anderson, Ph.D.Studied the potential use of stem cells to rehabilitate spinal cord injuries

(SCI) in mouse models1) Elucidate the potential therapeutic transplant window for cervical SCI at 9dpi and 60dpi using hCNS-SC in Rag2g mice2) Establish long-term safety/toxicology of hCNS-SC in unilateral cervical SCI ATN rats treated with anti-NK, ASGM1 antibody treatmentTechniques: Animal surgery and post-op care, perfusion, dissection, hESC cell culture, animal behavior analysis, histology, project documentation & organization, general lab organization

2011-2012 CIRM Intern, Department of Anatomy and Neurobiology, University of California, Irvine-Aileen Anderson, Ph.D.

Studied the potential use of stem cells to rehabilitate spinal cord injuries (SCI) in mouse models1) Analyzed the effects of human compliment protein C1q on hCNS-SC differentiation in vitro

2) Studied hCNS-SC survival implementing the tet on/tet off system in vitro Techniques: hCNS-SC culturing and differentiation, live cell imaging, microscopy, immunocytochemistry, immunohistochemistry, Western blot, Microtome tissue sectioning, mCNS-SC extraction and tissue culture

2010-2011 Undergraduate Student Researcher, Department of Natural Sciences and Mathematics, California State University, Long Beach-Houng-Wei Tsai,

Ph.D. Understand the neuroendocrine mechanisms that regulate reproductive function and behaviors at an epigenetic level 1) Studied the sexually dimorphic expression of Usp9x in the neonatal

mouse brain Techniques: Protein extraction, BCA assay, Western blot

Presentations and PublicationsLaura L. Dickey, Colleen L. Worne, Jessica L. Glover, Thomas Lane and Ryan O’Connell. “miR-155 enhances T-cell trafficking and antiviral effector function in a model of viral-induced neurological disease” (August 2016)

CIRM Trainee Conference-Poster Presentation“Concentration dependent effect of complement protein C1q on human neural stem cell proliferation, survival and differentiation in vitro” (July 2012)

International Stem Cell Awareness Day Symposium, UCI“Concentration dependent effect of complement protein C1q on human neural stem cell proliferation, survival and differentiation in vitro” (September 2012)3rd place in poster competition-cash prize

Leadership

*Lab management: Ensure laboratory compliance with university/state/federal regulations, manage and control laboratory budget, training of lab techniques/animal

surgeries/assays, perform experiments, develop/troubleshoot protocols, delegate lab duties, hire/train subordinates, write IACUC & IBC protocols, general lab maintenanceDeveloped Western blot quantification and live cell imaging protocols (UCI)Lead inter-lab collaboration to develop custom made microwells for live cell imaging (UCI)

Honors/AwardsStem Cell Awareness Day Poster Competition Finalist3rd place-cash prize

CIRM Trainee Grant2011

Special Skills Animal husbandry (mouse, rat)Spinal surgery (mouse, rat)Microscopy: Inverted, ConfocalMicrosoft OS (Excel, Word, PowerPoint), Mac OS (Prism, Numbers)Image capture & analysis: Metamorph, Volocity, Imaris, ImageJFigure Composition: Photoshop CS2/4/6, OmnigraffleVector-based Modeling Software: DraftSight