CURRICULUM VITAE

Education:

2011-2015 PhD in cell and molecular biology.

PhD defense: 16th of December, 2015.

Faculty of Medicine, University of Paris XI, France.

2009-2011 M.S. Biology and Health, thematic: Carcinogenesis.

Faculty of Medicine, University of Paris XI, France.

2006-2009 B.S. Biochemistry.

Faculty of Biology, University of Burgundy, Dijon, France.

Professional experiences:

2011-July 2016

PhD student (4 years).

Lab: UMR3348: Genotoxic stress and cancer. Curie Institute, Orsay, France. Team: Genetic instability and carcinogenesis. Thesis: Functional relationship between nucleotide pool and PARP-1: a new source of genetic instability. Supervisor: Dr. Mounira Amor-Guéret: mounira.amor@curie.fr

2010-2011 Internship (10 months). Lab: INSERM U866: Lipids, Nutrition, Cancer, Dijon, France. Proteomic Platform of CLIPP-FEMTO, Besançon, France. Team: Genetic regulation in hematopoiesis and leukemogenesis. Subject: Molecular characterization of cIAP1 interaction with its partners. Supervisors: Dr Laurence Dubrez-Daloz: ldubrez@u-bourgogne.fr Dr Wilfrid Boireau: wboireau@femto-st.fr

2009-2010 Internship (5 months).

Lab: INSERM U866: Lipids, Nutrition, Cancer, Dijon, France. Team: Genetic regulation in hematopoiesis and leukemogenesis. Subject: Role of cIAP1 in DNA lesion and repair. Supervisor: Dr Laurence Dubrez-Daloz: ldubrez@u-bourgogne.fr

GEMBLE Simon French 3 rue du mont Ventoux 91940 Les Ulis, France (33) 6.71.54.50.64 simon.gemble@gmail.com Born on 01/31/1989

Research activities:

Articles:

A balanced pyrimidine pool is required for optimal Chk1 activation to prevent ultrafine anaphase bridge formation. Gemble S, Buhagiar-Labarchède G, Onclercq-Delic R, Biard D, Lambert S, Amor-Guéret M. (2016) Journal of Cell Science.

Pyrimidine pool disequilibrium induced by a cytidine deaminase deficiency inhibits PARP-1

activity, leading to the under-replication of DNA. Gemble S, Ahuja A, Buhagiar-Labarchède G, Onclercq-Delic R, Dairou J, Biard D, Lambert S, Lopes M, Amor-Guéret M. (2015) Plos Genetics.

Cdk5 promotes DNA replication stress checkpoint activation through RPA-32

phosphorylation, and impacts on metastasis free survival in breast cancer patients. Chiker S, Pennaneach V, Loew D, Dingli F, Biard D, Cordelières F, Gemble S, Vacher S, Bieche I, Hall J, Fernet M. (2015) Cell Cycle.

cIAP1 regulates TNF-mediated cdc42 activation and filopodia formation. Marivin A, Berthelet J, Cartier J, Paul C, Gemble S, Morizot A, Boireau W, Saleh M, Bertoglio J, Solary E, Dubrez L. (2014) Oncogene.

Bloom's syndrome and PICH helicases cooperate with topoisomerase IIα in centromere disjunction before anaphase. Rouzeau S, Cordelières FP, Buhagiar-Labarchède G, Hurbain I, Onclercq-Delic R, Gemble S, Magnaghi-Jaulin L, Jaulin C, Amor-Guéret M. (2012) Plos One.

Cellular Inhibitor of Apoptosis Protein-1 (cIAP1) can regulate E2F1-mediated control of cyclin

transcription. Cartier J *, Berthelet J *, Marivin A, Gemble S, Edmond V, Plenchette S, Lagrange B, Hammann A, Dupoux A, Delva L, Eymin B, Solary E, Dubrez L. (2011) The Journal of Biological Chemistry.

Management & communications: Member in the organizing committee of « PhD day: your thesis at Curie Institute », Paris,

France, 2014.

Teaching to Master students from 2013 to 2015, Paris XI, Orsay. Course title: « Genetic instability and carcinogenesis: the Bloom syndrome ». Person in charge of the master: Simon Saule, UMR3347, Curie Institute, Orsay, France (simon.saule@curie.fr).

Students supervisor (trainee and PhD student).

Writing of scientific papers.

Posters:

Year: Titles: Authors: Places:

2014

Role of pyrimidine pool disequilibrium in ultrafine

anaphase bridge formation

Gemble S, Buhagiar-Labarchède G,

Onclercq-Delic R and Amor-Guéret

M

Young Researcher in Life

Science (YRLS), Paris, France

Young Researcher in Life

Science (YRLS), Paris, France

Journées scientifiques et médicales (JSM) of Curie

Institute, Paris, France

PARP-1 inactivation by

pyrimidine pool

disequilibrium leads to

ultrafine anaphase bridge

formation

Gemble S, Ahuja A, Buhagiar-

Labarchède G, Onclercq-Delic R,

Dairou J, Biard D, Lambert S, Lopes

M, Amor-Guéret M

Fusion Conference :

“DNA Replication as a

Source of DNA

Damage”, El Jadida,

Morocco

2013 Role of pyrimidine pool

disequilibrium in ultrafine anaphase bridge formation

Gemble S, Buhagiar-Labarchède G, Onclercq-Delic R and Amor-Guéret

M

Keystone symposia : “Genomic

Instability and DNA repair”, Banff,

Canada

Young Researcher in Life Science (YRLS), Paris,

France

Journées scientifiques

et médicales (JSM) of

Curie Institute, Paris,

France

2012

Bloom’s Syndrome and PICH

Helicases Cooperate with Topoisomerase IIa in

Centromere Disjunction Before

Anaphase

Rouzeau S, Cordelières FP, Buhagiar- Labarchède G, Hurbain I,

Onclercq-Delic R, Gemble S, Magnaghi-Jaulin L, Jaulin

C, Amor-Guéret M

Young Researcher in Life Science (YRLS), Paris,

France

Oral communications:

Year: Titles: Authors: Places:

2015

Genetic instability and carcinogenesis: a new

link between nucleotide pool disequilibrium and

PARP-1

Gemble S, Ahuja A, Buhagiar- Labarchède G, Onclercq-Delic R, Dairou

J, Biard D, Lambert S, Lopes M, Amor-Guéret

M

Journées scientifiques et médicales (JSM) of Curie Institute, Paris,

France

2016

Functional relationship

between nucleotide pool and PARP-1:

a new source of genetic instability

Gemble S, Buhagiar-Labarchède G, Onclercq-Delic R and Amor-Guéret

M

Institute of European Oncology, Milan, Italy

Centre de recherche en cancérologie, Marseille,

France

Skills: Scientific and technical: Cell and molecular biology: Cell culture (mammalian cells),

Immunofluorescence, FISH, transfection, flow cytometry, metaphase spreads, subcellular fractionation, Poly-ADP-Ribose detection by immunofluorescence, PARP-1 in vitro assay, qPCR, Western Blot.

Protein interaction: Biacore, Immunoprecipitation, GST Pull Down.

Others: Western Blot, optical microscopy.

Informatics: Office, BD FACS DIVA and Flow Jo (FACS software), Image J,

Metamorph.

Languages: French (maternal), English (intermediate).

References:

Dr. Mounira Amor-Guéret: mounira.amor@curie.fr Ph: (33) 1 69 86 30 53

Dr. Sarah Lambert: sarah.lambert@curie.fr Ph: (33) 1 69 86 71 91

Dr. Valérie Schreiber: valerie.schreiber@unistra.fr Ph: (33) 3 68 85 47 04

Dr. Jean-Sébastien Hoffmann: jean-sebastien.hoffmann@inserm.fr Ph: (33) 5 61 17 59 75