CURRICULUM VITAE (CV)

First name: Behrooz

Surname: Johari

Date, Place of Birth: MAR 4, 1987; Nahavand, Hamedan Province

Marital Status: Married, one children

Nationality: Iranian

CONTACT PROFESSIONAL ADDRESSES

Address: Department of medical biotechnology and nanotechnology, Zanjan University of

medical science, medical school, zanjan city

Mobile: (0098) 939 3724494, E-mail: behroozjohari@yahoo.com

QUALIFICATION

PhD in Medical Biotechnology, (2012-2017) Project title: Using c-Myc transcription factor

oligodeoxynucleotide decoys to inhibit stemness in mouse embryonic stem cells, Department of

Biochemistry, Pasteur Institute of Iran, Tehran, Iran.

MSc in Medical Biotechnology, (2010-2012) Project title: Study of the effects of a gelatin-

hydroxyapatite Nano-composite with osteoblast and endothelial cells in the management of an

experimental model of critical bone defect in rats, Department of Medical Biotechnology, Faculty of

Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran.

BSc in surgical Technology (Operating room), (2008-2010) Department of surgical

Technology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.

Associate degree in surgical Technology (Operating room), (2006-2008) Department of

surgical Technology, Faculty of Allied Medicine, Kermanshah University of Medical Sciences,

Kermanshah, Iran.

HONORs and AWARDs

1. Top Student in the class medical biotechnology, Tehran University of Medical Sciences,

Tehran, Iran (2012).

2. Top Student among students of Faculty of Allied Medicine, Iran University of Medical

Sciences, Tehran, Iran (2010).

3. Talented Student in Faculty of Allied Medicine, Iran University of Medical Sciences,

Tehran, Iran (2008-2010).

4. Talented Student in Faculty of Allied Medicine, Kermanshah University of Medical

Sciences, Kermanshah, Iran (2006-2008).

5. Top Student in the class Experimental Sciences at shahid beheshti highschool, Nahavand,

Hamedan, Iran (2002-2006).

INTERESTS

Tissue engineering, Cell signaling, Differentiation therapy, Cancer research

RESEARCH EXPERIENCES and SKILLS

In vitro, Cell culture (stem cell/cell line), Tissue preparation for Histology,

Immunohistochemistry (IHC), Light, fluorescent and confocal microscopy, cytotoxicity

assay, Tissue engineering scaffold synthesis, RNA & DNA extraction, PCR, Real-time PCR,

Electrophoresis (SDS PAGE), EMSA, Western blotting etc.

In vivo, Animal working include handling, injection and surgery.

Analytical techniques: Bioinformatics tools (primer3, oligoanalyzer), Statistics Software

(Microsoft Excel, Graph Pad Prism…), ICDL etc.

TEACHING COURSES

Cellular and molecular biology, Cell culture technics, Immunology, Medical genetics, Medical

biotechnology, Molecular biotechnology.

PUBLICATIONS

BOOKS (In Farsi)

Images of Book

Title of Books

Publisher and date of publication

Medical Biotechnology

Bernard Glick

Under publishing

Immunology For Pharmacy -Dennis K.

Flaherty- 2012

Eshraghie (2016)

Gene Cloning & DNA Analysis Eshraghie (2017)

An introduction to 3D cell culture animal

cell& tissue engineering

Eshraghie (2017)

Molecular biotechnology- Bernard R Glick,

Jack J Pasternak-2010

Eshraghie (2016)

Essential of Cellular & Molecular Biology&

Genetic Engineering

Eshraghie (2014)

Q&A of Cellular& Molecular Biology&

Genetics Engineering

Eshraghie (2015)

Principal dimensional culture of animal cells Eshraghie (2016)

Clinical Biochemistry (DRS) Jameenegar (2015)

Practical Principle of Microbiology

Laboratory

Jameenegar (2015)

Guideline for laparoscopy& Thoracoscopy

surgery

Khosravi (2012)

Surgical Technologies & Scrub Andishe-rafi (2012)

JOURNALS (Full text in English)

1. Zohreh Saltanatpour, Behrooz Johari, Akram Alizadeh, Majid Lotfinia,

Keivan Majidzadeh‐A, Behrooz Nikbin, Mehdi Kadivar, Enrichment of cancer stem‐like

cells by the induction of epithelial‐mesenchymal transition using lentiviral vector

carrying E‐cadherin shRNA in HT29 cell line. May 2019, journal of cellular physiology.

2. Zahra Bigdelou, Behrooz Johari, Mehdi Kadivar, Elham Rismani, Zoleykha Asadi,

Mohammad Rahmati, Zohreh Saltanatpour. Investigation of specific binding of designed

oligodeoxynucleotide decoys to transcription factors in HT29 cell line undergoing

epithelial–mesenchymal transition (EMT), April 2019, journal of cellular physiology.

3. Behrooz Johari, Zoleykha Asadi, …., and Mehdi Kadivar. Inhibition of transcription

factor T‐cell factor 3 (TCF3) using the oligodeoxynucleotide strategy increases

embryonic stem cell stemness: possible application in regenerative medicine, Cell Biol

Int (2019).

4. Mohsen Mousavi, Jamil Zargan,..., Behrooz Johari. Anticancer effects of the

Latrodectus dahli crude venom on MCF‐7 breast cancer cell line. April 2018, the breast

journal.

5. Leila SAREMI, …, Behrooz JOHARI, Zohreh SALTANATPOUR. Association

between PPARGC1A single nucleotide polymorphisms and increased risk of

nonalcoholic fatty liver disease among Iranian patients with type 2 diabetes mellitus , Turk J Med Sci (2019).

6. Maryam Farshadi, Behrooz Johari,…, Reza Nekouian and Ali Samadikuchaksaraei.

Nanocomposite scaffold seeded with mesenchymal stem cells for

bone repair, Cell Biol Int (2019).

7. Leila Saremi, Shirin Lotfipanah,…, Behrooz Johari and Zohreh Saltanatpour. The Pro12Ala

polymorphism in the PPARγ2 gene is not associated with an increased risk of NAFLD

in Iranian patients with type 2 diabetes mellitus. Cellular & Molecular

Biology Letters (2019).

8. Mohammad Moradi, Mansoreh Abdolhosseini, Ali Zarrabi, Behrooz Johari. A review on

application of Nano-structures and Nano-objects with

high potential for managing different aspects of bone malignancies, Nano-Structures &

Nano-Objects 19 (2019).

9. Behrouz Farhadihosseinabadi, Fahimeh Hosseini,…, Behrooz Johari, Meghdad

Abdollahpour-Alitappeh. Breast Cancer: Risk Factors, Diagnosis and Management, Medical Laboratory Journal, Sep-Oct , 2018; Vol 12: No 5.

10. Mohsen Mousavi , Behrooz Johari , Jamil Zargan, …, Investigating Antibacterial

Effects of Latrodectus Dahli Crude Venom on Escherichia coli, Staphylococcus aureus

and Bacillus subtilis, Medical Laboratory Journal, May-Jun, 2019; Vol 13: No 3.

11. Mansure Kazemi, Mahmoud Azami, Behrooz Johari, …, Mohammad Farajollahi , Bone

Regeneration in rat using a gelatin/bioactive glass nanocomposite scaffold along with

endothelial cells (HUVECs), March 2018, international journal of applied ceramic

technology.

12. Ali Rahiminejad, Rassoul Dinarvand, Behrooz Johari,…, Masood Khosravani,

Preparation and investigation of indirubin-loaded SLN nanoparticles and their anti-

cancer effects on human glioblastoma U87MG cells, Cell Biol Int 43 (2019.

13. Meghdad Abdollahpour‐Alitappeh, Majid Lotfinia, …, Behrooz Johari, Mohammad

Reza Zali, Nader Bagheri, Antibody–drug conjugates (ADCs) for cancer therapy:

Strategies, challenges, and successes, August 2018, journal of cellular physiology.

14. Nastaran Ghahhari, Faezeh Maghsood, Saeed Jahandideh, Behrooz Johari, Mehdi

Kadivar. Secretome of Aggregated Embryonic Stem Cell-Derived Mesenchymal Stem

Cell Modulates the Release of Inflammatory Factors in Lipopolysaccharide-Induced

Peripheral Blood Mononuclear Cells. Nov 2017, Iranian biomedical journal.

15. Saeed Jahandideh, Faezeh Maghsood, Nastaran Ghahhari, Behrooz Johari, Mehdi

Kadivar. The effect of Trimetazidine and Diazoxide on immunomodulatory activity of

human embryonic stem cell-derived mesenchymal stem cell secretome. 2017, Tissue and

Cell, 49(5):597-602.

16. Behrooz Johari, Jamil Zargan. Simultaneous targeted inhibition of Sox2-Oct4

transcription factors using decoy oligodeoxynucleotides to repress stemness properties

in mouse embryonic stem cells. 2017, Cell biology international. 41(12):1335-1344. (Impact

Factor:1.8)

17. Adel M, Zahmatkeshan M, Johari B, Kharrazi S, Mehdizadeh M, Bolouri B, Rezayat SM.

Investigating the effects of electrical stimulation via gold nanoparticles on in vitro

neurite outgrowth: Perspective to nerve regeneration. Microelectronic Engineering. 2017 Apr

5; 173:1-5. (Impact Factor:1.8)

18. Johari B, Ebrahimi-Rad M, Maghsood F, Lotfinia M, Saltanatpouri Z, Teimoori-Toolabi

L, Sharifzadeh Z4, Karimipoor M3, Kadivar M. Myc Decoy Oligodeoxynucleotide

Inhibits Growth and Modulates Differentiation of Mouse Embryonic Stem Cells as a

Model of Cancer Stem Cells. Anticancer Agents Med Chem. 2017 Apr 12. doi:

10.2174/1871521409666170412142507. (Impact Factor:2.7)

19. Behrooz Johari, Mehdi Kadivar, Shirin Lak, Mazaher Gholipourmalekabadi, Aleksandra

M. Urbanska, Masoud Mozafari, Maryam Ahmadzadehzarajabad, Asaad Azarnezhad,

Samane Afshari, Jamil Zargan, Saeid Kargozar, Osteoblast–seeded bioglass/gelatin

nanocomposite: a promising bone substitute in critical-size calvarial defect repair in

rat, Int J Artif Organs 2016; 39(10): 524-533. (Impact Factor:1.005)

20. Zohreh Saltanatpour, Mehdi Kadivar, Behrooz Johari, Majid Lotfinia, Faezeh Maghsood,

Sirous Zeinali, Leila Nasehi, Ali Fallah, Aslan Fanni, Behrooz Nikbin, Transduction of an

optimized recombinant lentivirus expressing E-cadherin shRNA resulted in stable

downregulation of CDH1 gene and obvious cell morphological change in the human colorectal

cancer cell line HT29, Health Sciences 5.11 (2016): 87-93.(Impact Factor: 0.05)

21. A Azarnezhad, Z Sharifi, R Seyedabadi, A Hosseini, B Johari, Cloning and Expression of

Soluble Recombinant HIV-1 CRF35 Protease-HP Thioredoxin Fusion Protein, Avicenna Journal

of Medical Biotechnology 8.4 (2016): 175. (Impact Factor: 0.5)

22. Behrooz Johari, Maryam Ahmadzadehzarajabad, Mahmoud Azami, Mansure Kazemi,

Mansooreh Soleimani, Saied Kargozar, Saieh Hajighasemlou, Mohammadmorad

Farajollahi, Ali Samadikuchaksaraei, Repair of Rat Critical Size Calvarial Defect Using

Osteoblast-like and Umbilical Vein Endothelial Cells seeded in Gelatin/Hydroxyapatite

Scaffolds, J Biomed Mater Res A. 2016 Mar 17. DOI:10.1002/jbm.a.35710. (Impact Factor:3.5)

23. Ali Samadikuchaksaraei, Mazaher Gholipourmalekabadi, Elham Erfani Ezadyar,

Mahmoud Azami, Masoud Mozafari, Behrooz Johari, Saeid Kargozar, Seyed

Behnamedin Jameie, Alireza Korourian and Alexander M. Seifalian, Fabrication and In

vivo evaluation of an osteoblast-conditioned nano-hydroxyapatite/gelatin composite

scaffold for bone tissue regeneration, J Biomed Mater Res A. 29 MAR 2016. DOI:

10.1002/jbm.a.35731. (Impact Factor: 3.5)

24. Saieh Hajighasemlou, Mahmoud Alebouyeh, Hossein Rastegar, Mojgan Taghizadeh

Manzari, Milad Mirmoghtadaei, Behjat Moayedi, Maryam Ahmadzadeh, Farzad

Parvizpour, Behrooz Johari, Maria Moslemi Naeini, Mohammad M Farajollahi,

Preparation of Immunotoxin Herceptin-Botulinum and Killing Effects on Two Breast

Cancer Cell Lines, APJCP.2015.16.14.5977.(Impact Factor: 2.5)

25. Lotfinia, M., Lak, S., Mohammadi Ghahhari, N., Johari, B., Maghsood, F., Parsania, S. &

Kadivar, M., Hypoxia Pre-Conditioned Embryonic Mesenchymal Stem Cell Secretome

Reduces IL-10 Production by Peripheral Blood Mononuclear Cells. 2017, Iranian biomedical

journal.(Impact Factor: 0.0)

26. Fatemeh Nasiri, Behrooz Johari, Fatemeh Amiri, Mehryar Habibi Roudkenar, Sedigheh

Molaei, Marzie Bahadori, Marjan Movahed, Abazar Yari, Mohammad Ali Jalili, H2O2-

preconditioned umbilical cord-derived mesenchymal stem cells ameliorate liver regeneration in

acute liver failure-induced mice. Anatomical Sciences.2016; 13(3):183-190.

27. Sangarifar S, Ghajavand H, Johari B, Yari A. Frequency of clf-A, mec-A, and mec-C Genes

in Staphylococcus Aureus Strains Isolated From Nosocomial Infections and Cow’s Milk. Anatomical Sciences. 2016; 13(4):233-238.

RESEARCH PROJECTS

1. Bone healing effects of a tissue engineered gelatin hydroxyapatite Nano- composite seeded

with osteoblast and endothelial cells in the management of an experimental modal of

critical size- bone defect in rat.)code: 16240)

2. An osteoblast seeded gelatin hydroxyapatite Nano- composite for the repair of an

experimental modal of critical size- bone defect.(code: 16234)

3. Enhancement of bone healing properties of a Bioglass-gelatin composite with endothelial

cells.( code: 16227)

4. Investigation the effects of electrical stimulation via gold nanoparticles for in vitro nerve

regeneration.( code: 18035)

WORKSHOPS ATTENDED

1. CLC main workbench, Pasteur institute of Iran (2016).

2. Antibody design workshop, which held by Rastegar reference laboratory of faculty of

veterinary medicine, university of Tehran (2016).

3. Immunoinformatics workshop, which held by EMGEN in collaboration with HBC (2014).

4. Chemoinformatics workshop, which held by EMGEN in collaboration with HBC (2014).

5. Bioinformatics as title education oligo7& allele ID Software workshop, Pasteur institute of

Iran (2014).

6. Flowcytometry workshop, Pasteur institute of Iran (2015).

7. GMP (Good Manufacturing Practice) and GLP (Good Laboratory Practice) workshop,

Pasteur institute of Iran (2015).

8. Based on criticism reflection in medical science, Iran university medical science (2010).

9. Medical education according to best observation, Iran university medical science (2010).

10. Student network of EBM, Iran university medical science (2010).

RECENT SEMINAR AND CONGRESS

1. 1397 (2018), Inhibition of T cell factor (Tcf3) transcription factor using

oligodeoxynucleotide decoy strategy increases embryonic stem cell stemness, The First

International Iranian Tissue Engineering and Regenerative Medicine Congress, 18-20

July, Tehran, Iran. .(Oral presentation)

2. 1397 (2018), Synthesis, characterization and in vivo evaluation of gelatin-hydroxyapatite-

silicon carbide nanocomposite scaffold seeded with mesenchymal stem cells for bone

tissue repair, The First International Iranian Tissue Engineering and Regenerative

Medicine Congress, 18-20 July, Tehran, Iran. (Poster presentation).

3. 1397 (2018), A STAT3-Decoy Oligodeoxynucleotide Suppresses Cell Growth and

Induces Apoptosis in Erlotinib Resistant SW480 Colon Cancer Cell Line by Blockage of

the STAT3 Signaling Pathway, International congress on stem cell& regenerative

medicine, 28-30 November, Tehran, Iran. (Poster presentation).

4. 1397 (2018), Induction of Mesenchymal-Epithelial Transition (MET) in HT29 Cell Line

Containing Cancer Stem-Like Cell (HT29-shE) with Differentiation Therapy Purpose for

Cancer Treatment, International congress on stem cell& regenerative medicine, 28-30

November, Tehran, Iran. (Poster presentation).

5. 1396 (2017), Myc Decoy Oligodeoxynucleotide Inhibits Growth and Modulates

Differentiation of Mouse Embryonic Stem Cells as a Model of Cancer Stem Cells,

international congress on stem cell& regenerative medicine, 13-15 July, Tehran,

Iran.(Oral presentation)

6. 1395 (2016), Osteoblast–seeded bioactive glass/gelatin nanocomposite for the repair of

an experimental model of critical-size bone defect, 3nd Iranian Congress on Progress in

Tissue Engineering and Regenerative Medicine, 28-30 Mehr, Tehran, Iran.(Oral

presentation).

7. 1395 (2016), Osteoblast–seeded bioglass/gelatin nanocomposite: a promising bone

substitute in critical-size calvarial defect repair in rat. 12th Royan International Congress

on Stem Cell Biology & Technology, Royan Institute, Tehran, Iran.(Poster

presentation).

8. 1394 (2015), Enhanced Repair of Rat Critical Size Calvarial Defect Using Osteoblast-like

and Umbilical Vein Endothelial Cells seeded in Hydroxyapatite/Gelatin Scaffolds. 2nd

Iranian Congress on Progress in Tissue Engineering and Regenerative Medicine.16-18

Aban, Tehran, Iran.(Oral presentation).

9. 1391 (2012), Investigating the Effects of Electrical Stimulation via Gold Nanoparticles

for In Vitro Nerve Regeneration. The First Iranian Annual Congress on Progress in Tissue

Engineering and Regenerative Medicine 30 ordibehesht, Tehran, Iran.(Poster

presentation).

10. 1391 (2012), An Osteoblast-Seeded Gelatin-Hydroxyapatite Nano-Composite for the

repair of an Experimental Model of Critical-Size Bone Defect. The First Iranian Annual

Congress on Progress in Tissue Engineering and Regenerative Medicine 30 ordibehesht,

Tehran, Iran.(Poster presentation).

11. 1391 (2012), Bone Healing Effects of a Tissue-Engineered Gelatin-Hydroxyapatite Nano-

Composite Seeded with Osteoblasts and Endothelial Cells in the Management of an

Experimental Model of Critical-Size Bone Defect in Rats. The First Iranian Annual

Congress on Progress in Tissue Engineering and Regenerative Medicine 30 ordibehesht

,Tehran, Iran.(Poster presentation).

12. 1391 (2012), Enhancement of Bone Healing Properties of a Bioglass-Gelatin Composite

with Endothelial Cells. The First Iranian Annual Congress on Progress in Tissue

Engineering and Regenerative Medicine 30 ordibehesht ,Tehran, Iran.(Poster

presentation).

13. 1390 (2011), Attend in the first MBA- Biotechnology congress 23 Azar, Tehran, Iran.

14. 1392 (2013), Attend in the second MBA- Biotechnology congress 20 ordibehesht,

Tehran, Iran.

15. 1392 (2013), Attend in the first Iranian annual congress, 30 ordibehesht, Tehran, Iran.

16. 1389 (2010), Attend in the eleventh medical education congress, 10-11 ordibehesht,

Tehran, Iran.

HOBBIES

Football, Volleyball, Traveling, Reading.

LANGUAGES

Persian (mother tongue), English (average), Arabic (not advanced).

REFERENCES

1. Ali Samadikuchaksaraei, MD, PhD, DIC, CWC, MIRIMC, FRIPH

Consultant in Regenerative Medicine and Tissue Engineering Consultant in General Medicine

Associate Professor (Lecturer)

Department of Biotechnology

Cellular and Molecular Research Center (CMRC)

Tehran University of Medical Sciences

PO Box: 14155/6183

Tehran, Iran

Tel: (+98 21) 8805 2984

Mobile: (+98 912) 55-77-66-9

Fax: (+98 21) 8805 4355

+1 530 380 7337 (Web-based, California)

+44 870 913 4099 (Web-based, UK)

Emails: ali.samadi01@imperial.ac.uk

samadikuchaksaraei@yahoo.com

2. Mehdi Kadivar, PhD of medical biotechnology, Associate Professor

Biochemistry Department, Pasteur institute of Iran

Tel: (+98 21) 669-69298

Mobile: (+98 912) 412-8860

Fax: (+98 21) 664-65132

Email: Kadivar@pasteur.ac.ir

3. Mina Ebrahimi-Rad, PhD of medical biotechnology, Assistant Professor

Biochemistry Department, Pasteur institute of Iran

Tel: (+98 21) 641-12178

Mobile: (+98 912) 325-7479

Fax: (+98 21) 664-65132

Email: minebrad@pasteur.ac.ir