Creating Microaerophilic Atmospheric Condition for Incubation of C. jejuni Dr. Abdulaziz Bamarouf.
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Transcript of Creating Microaerophilic Atmospheric Condition for Incubation of C. jejuni Dr. Abdulaziz Bamarouf.
Creating Microaerophilic Atmospheric Condition for
Incubation of C. jejuni
Dr. Abdulaziz Bamarouf
Microaerophilic
• An atmosphere of 5% O2 is appropriate for culturing C. jejuni
•This microaerophilic atmosphere can be produced by several ways including:
Candle Jar
• The oxygen concentration in a sealed jar is reduced by the burning candle and produces a microaerophilic atmosphere
Candle Jar
Evacuation Replacement System
• The air in a sealed jar is removed by a vacuum device and produces a microaerophilic atmosphere and then replaced with a special gas mixture
Evacuation Replacement System
Fortner Principle
•Campylobacter agar plate is sealed in a plastic bag with a second agar plate inoculated with E. coli
•Because E. coli is facultative anaerobe, it uses up some of the oxygen in the bag and produces a microaerophilic atmosphere
Fortner Principle
Gas Generating Systems
• The oxygen concentration in a sealed jar is reduced by using gas-generating systems
• Gas-generating systems are activated by adding water to a special envelope which contain tablets containing different chemicals
•A chemical reaction will start which consume oxygen and produces a microaerophilic atmosphere
Gas Generating Systems
Gas Generating Systems
Gas Generating Systems
Microaerophilic Bags
• Microaerophilic bags are commercially available
•Agar plate and microaerophilic gas-generating system are sealed in a disposable, gas-impermeable plastic bag
•Gas-generating systems are activated by crushing a special ampule
Microaerophilic Bags
•A chemical reaction will start which consume oxygen and produces a microaerophilic atmosphere
Anaerobic Chambers
• This is optimal incubation system
• It allows all laboratory manipulation to occur in controlled atmospheric condition
•Chambers equipped with airtight rubber gloves for the technologist to perform their work inside the chamber
Anaerobic Champers
A= gas mixture B= entry lock F= incubator
Anaerobic Champers
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