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Campylobacter jejuni Epidemiology and Evolution
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Transcript of Campylobacter jejuni Epidemiology and Evolution
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Campylobacter jejuni
Epidemiology and Evolution
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Veterinary Training Research InitiativeFood-borne zoonotic pathogens:Transmission, pathogen evolution and control
Lancaster University Paul Fearnhead, Edith Gabriel, Peter Diggle
University of Liverpool Howard Leatherbarrow, Tony Hart, Malcolm Bennett
Health Protection Agency Andrew Fox, Steve Gee, Sam James John Cheesbrough, Eric Bolton
Funding: DEFRA (Department for Environment, Food and Rural Affairs, UK
Government)
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Characterisation ofC. jejuni infection
• Epidemiology
• Evolutionary history
• Population genetics
• Source of human cases
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Foodborne illness in the UKFood Standards Agency figures for 2000
Salmonella 16,987 20.9%
Campylobacter 62,867 77.3%
E.coli O157 1,147 1.4%
Clostridium perf. 166 0.2%
Listeria 113 0.1%
Total 81,280
$8bnAnnual cost to US economy
Buzby et al. JID (1997)
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Cases and controls RiskSignificance
Poisson point process
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Seasonal patterns
Harmonic regression
25.4% reduction year-on-year
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tkt 459bp
glyA 507bp489bp uncA
498bp pgm
402bp gltA
477bp glnA
477bp aspA
1.6 million bp genome
MLST: 3300 bp in total(0.2%)
MLST: Multi-locus sequence typing
Multi-locus sequence typing (MLST)
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0
20
40
60
80
100
120
140
21 257 48 104 45 53 50 19 61 574
Sequence Type
Abundance
21
257
48104
4553
50
1961
574
Multi-locus sequence typing (MLST)
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ST 21: 2 1 1 3 2 1 5
ST 104: 2 1 1 3 7 1 5
ST 50: 2 1 12 3 2 1 5
ST 21: 2 1 1 3 2 1 5
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Figure 1. Number of nucleotide differences between each pairs of alleles at the seven loci. When C. coli-derived alleles are removed, the red portion of the histogram disappears.
Genetic inhomogeneity in Campylobacter jejuni
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Campylobacter
jejuni
Campylobactercoli
Common ancestor
gene flow
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Putatative Campylobacter jejuni isolates
Putatative Campylobacter coli isolates
ST aspA glnA gltA glyA pgm tkt uncA
21 2 1 1 3 2 1 5
257 9 2 4 62 4 5 6
66 2 4 5 2 7 1 5
61 1 4 2 2 6 3 17
ST aspA glnA gltA glyA pgm tkt uncA
21 2 1 1 3 2 1 5
Allele frequency
C. jejuni 0.33 0.22 0.15 0.2 0.23 0.29 0.29
C. coli 0 0 0 0 0 0.01 0
ST aspA glnA gltA glyA pgm tkt uncA
257 9 2 4 62 4 5 6
Allele frequency
C. jejuni 0.07 0.14 0.08 0.05 0.05 0.07 0.17
C. coli 0 0 0 0 0 0 0
ST aspA glnA gltA glyA pgm tkt uncA
825 33 39 30 82 113 47 17
Allele frequency
C. jejuni 0.01 0.01 0.01 0.01 0 0 0.04
C. coli 0.64 0.49 0.62 0.46 0.18 0.15 0.48
ST aspA glnA gltA glyA pgm tkt uncA
61 1 4 2 2 6 3 17
Allele frequency
C. jejuni 0.13 0.14 0.15 0.18 0.06 0.18 0.04
C. coli 0 0 0.01 0.01 0 0.01 0.48
Population Structure: identifying hybrids
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C. jejuni – C. coli hybrids3 sequence types, 20/881 isolates (2.2%)
Population Structure: identifying hybrids
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Campylobacter
jejuni
Campylobactercoli
Common ancestor
gene flow
aspA-33pgm-93uncA-17
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Coalescent modelling with ABC
QuickTime™ and a decompressor
are needed to see this picture.
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QuickTime™ and a decompressor
are needed to see this picture.
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Campylobact
erjejuni
Campylobacter
coli
2002
20001998
1592(1772
-1305)
1966
MRCA
uncA-17
pgm-93
aspA-33
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Campylobacter jejuni Campylobacter coli
Common ancestor
gene flow
aspA-33pgm-93uncA-17
present
4 500 BC(1 500 -10 000
BC)Domestic pig domestication in the Paris Basin, circa 4000 BC
Larson et al. PNAS (2007)
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9.9 m
6.6 m
9.8 m
24 m
32 m
51 m
7.5 m years
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CATTLE
SHEEP
CHICKEN
PIG
BIRD
ENVIRONMENT
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CATTLE
SHEEP
CHICKEN
PIG
BIRD
ENVIRONT
HUMAN
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CATTLE cattle 212beef offal or meat 47calf 12cows milk 11calf faeces 1cattle faeces 1 284
CHICKEN chicken 222chicken offal or meat 153chick 17 392
BIRD wild bird 172starling 71goose faeces 25turkey 22goose 12duck 7starling faeces 4 313
ENVIRONMENT sand (bathing beach) 52environmental waters 28soil 3potable/drinking water 2 85
SHEEP sheep 84lamb offal or meat 74lamb 10sheep faeces 2 170
PIG pig 35pork offal or meat 10piglet 1 46
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broiler environment 17cat 3dog 5farm slurry 10gazelle 1giraffe 1goat 5horse 1human blood culture 57human stool 1684human unspecified 102marmoset 2ostrich 1other animal 25rabbit 3unspecified 144
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BIRD
CATTLE
CHICKEN
ENVIRONMENT
PIG
SHEEP
Haplotype structure in sequences of known originfrom pubMLST
origin
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BIRD
CATTLE
CHICKEN
ENVIRONMENT
PIG
SHEEP
Haplotype structure in human isolates
key
NOVEL
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BIRD
CATTLE
CHICKEN
ENVIRONMENT
PIG
SHEEP
Haplotype structure in human isolates
key
NOVEL
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Attributing novel genotypes ST 574: 7 53 2 10 11 3 3 Human-specific, but similar to...
ST 305: 9 53 2 10 11 3 3 ST 713: 12 53 2 10 11 3 3 ST 728: 4 53 2 10 10 3 3 ST 2585: 7 2 3 10 11 3 3
All these found in chicken, so the likely source is chicken
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CATTLE
SHEEP
CHICKEN
PIG
BIRD
ENVIRONMENT
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CATTLE
SHEEP
CHICKEN
PIG
BIRD
ENVIRONT
HUMAN
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Model C: unlinked
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Does it work?Empirical cross-validation
Split sequences of known origin into two groups. Treat one group as having unknown origin (pseudo-human cases)
Infer the proportion of pseudo-human cases drawn from each source population
Repeat 100 times to study the performance of the method
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Simulation and empirical cross-validationResults: Linked model
Unlinked model Linked modelPredicted Correct 0.86 0.66Actual Correct 0.56 0.58Coverage CATTLE 22 100
CHICKEN 85 82BIRD 81 100
ENVIRONMENT 63 99SHEEP 16 98
PIG 89 94Combined 18 95
Bias CATTLE -0.13 0.00CHICKEN -0.01 -0.05
BIRD -0.02 -0.02ENVIRONMENT -0.01 0.00
SHEEP 0.16 0.06PIG 0.01 0.00
RMSE CATTLE 0.14 0.08CHICKEN 0.03 0.05
BIRD 0.03 0.04ENVIRONMENT 0.03 0.04
SHEEP 0.17 0.09PIG 0.01 0.01
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Case-by-case: probability of source
CATTLE
SHEEP
CHICKEN
PIG WILD BIRDENVIRONMENT
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Gene flow between source populations
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Tracing the source of infection: results
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Tracing the host species of humanfood-borne infections
Infected meat principal source (97%)
No evidence for environment or wild birds as a major transmission route
Prevention strategies: Enhanced on-farm biosecurity Interrupting transmission chain