Crystallization of Sodium Chlorate with d-Glucose Co-Solute Is Not
Conversion of Glucose to Sodium Glucanate in Bubble
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Transcript of Conversion of Glucose to Sodium Glucanate in Bubble
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Bioconversion of glucose
to gluconic acid.
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Experiment 4
Aim: To study the conversion of glucose to gluconic acidusing 1% soluble glucose oxidase and catalase
Reaction parameters:
Column volume 700ml
Glucose oxidase loading % ( )1 7ml
Catalase loading % ( )1 7ml
NaOH concentration 1 M
( )Substrate glucose
concentration
/100 mg ml
Substrate feed rate . /1 5 ml min
NaOH feed rate . /0 5 ml min
Permeate flow rate . /1 9 ml min
Residence time . ( . )466 75 min 7 8 hrs
:Reaction parameters
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Results
( )Time hr / . ( / )O L Glucose conc mg ml % Conversion
8 .37 23 .52 84
9 .38 95 .50 66
10 .37 42 .52 6111 .42 49 .46 18
12 .33 57 .57 48
13 .39 17 .50 39
14 .38 25 .51 55
15 .36 03 .54 36
16 .36 22 .54 13
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Results
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Experiment 5
Aim: To study the conversion of glucose to gluconic acidusing 0.5% soluble glucose oxidase and catalase.
Reaction parameters:
Column volume 1400 ml
Glucose oxidase loading . % ( )0 5 7ml
Catalase loading % ( )1 14ml
NaOH concentration 1 M
( )Substrate glucoseconcentration
/100 mg ml
Substrate feed rate . /1 5 ml min
NaOH feed rate /1 ml min
Permeate flow rate . /2 5 ml min
Residence time . ( . )933 33 min 15 55 hrs
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ResultsDate
( )Time hr
/ . ( / )O L Glucose conc mg ml
%Conversion
/ /31 08 09 1 .1 78 .97 38
2 .3 12 .95 423 .4 52 .93 37
4 .6 24 .90 855 .8 85 .87 016 .8 98 .86 83
/ /1 9 2009 0 .6 43 .90 56
1 .8 09 .88 142 .8 98 .86 833 .10 32 .84 874 .9 81 .85 615 .10 00 .85 33
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Results
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Experiment 6
Aim: To study the conversion of glucose to gluconic acidusing 2% soluble glucose oxidase and catalase.
Reaction parameters:
Column volume 700 ml
Glucose oxidase loading % ( )2 14ml
Catalase loading % ( )2 14ml
NaOH concentration 1 M
( )Substrate glucose concentration /100 mg ml
Substrate feed rate /7 ml min
NaOH feed rate . /0 7 ml min
Permeate flow rate . /7 6 ml min
Residence time
( . )100 min 1 66 hrs
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Results
( )Time hr / .O L Glucose conc( / )mg ml
% Conversion
1 .28 34 .69 23
2 .45 20 .50 93
3 .60 00 .34 86
4 .60 25 .34 58
5 .63 14 .31 44
8.
61 51.
33 22
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Results
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Experiment 7
Aim: To study the conversion of glucose to gluconic acidusing 3% soluble glucose oxidase and catalase.
Reaction parameters:
Column volume 700 ml
Glucose oxidase loading % ( )3 21mlCatalase loading % ( )3 21ml
NaOH concentration 1 M
( )Substrate glucose concentration /100 mg ml
Substrate feed rate /3 ml min
NaOH feed rate . /0 4 ml min
Permeate flow rate . /3 5 ml min
Residence time . ( . )233 33 min 3 88 hrs
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Results
( )Time hr / . ( / )O L Glucose conc mg ml % Conversion
1 .18 68 .78 20
2 .41 79 .51 24
3 .48 85 .43 01
4 .58 78 .31 42
5 .48 97 .42 86
6 .57 76 .32 62
7 .58 40 .31 87
8 .57 44 .32 99
9 .54 87 .35 98
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Results
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Result SummaryResidence
timeColumn
VolumeGOD
loadingCAT
loading%Avg
ConversionFlow
/ml min.Glucose Conc
( / )mg ml
Experiment 4 .7 8 hr 700 ml 7 ml 7 ml %54 .1 5 100Experiment 5 .15 55 hr 1400 ml 7 ml 14 ml %86 .1 5 100Experiment 6 .1 66 hr 700 ml 14 ml 14 ml %34 7 100Experiment 7 .3 88 hr 700 ml 21 ml 21 ml %32 3 100
xygen Supplied :/Moles min /mMoles min
Areation Rate .1 17 LPM .0 0519 .51 9O2Rate .0 245 LPM .0 0109 .10 9
oretical Oxygen Required :.Exp Glucose Rate /mMole min /Oxygen Required mMole min
4 /150 mg ml .0 83 .0 415
5 /150 mg ml .0 83 .0 415
6 /700 mg ml .3 88 .1 94
7/
300 mg ml.
1 66.
0 83
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Experiment 8
Effect of methanol concentration on the Glucose Oxidaseand Catalase mixture Activity
Results:
( + )Initial mixture GOD CAT Activity . /1379 6296 Units ml
( + )Mixture GOD CAT Activity in%presence of 10 methanol
. /1425 9259 Units ml
( + )Mixture GOD CAT Activity in%presence of 20 methanol
. /1462 9629 Units ml
( + )Mixture GOD CAT Activity in%presence of 30 methanol
. /1481 4814 Units ml
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Experiment 9
Effect of membrane on the Glucose oxidase and Catalasemixture activity
Parameters:
Membrane Material Polyethersulfone
Membrane Cut off 30 kD
Ultra filtration area 20 cm2
Column Volume 700 ml
GOD loading % ( )1 7 ml
CAT loading % ( )1 7 ml
Permeate flow rate /7 ml min
Residence time 100 min
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Results
( + )Initial mixture GOD CAT Activity . /1379 6296 Units ml
( + )Initial mixture GOD CAT Activityof retented
. /1268 5185 Units ml
( + )Final mixture GOD CAT Activity( )after 16 hours
. /1166 6666 Units ml
