Connon Thomas scanning em portfolio.pptx
Transcript of Connon Thomas scanning em portfolio.pptx
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Connon T. SEM Por,olio
12/10/14
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Part 1
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Cri$cal point drying – Specimen fixed in 2.5% glut, rinsed in sodium cacodylate buffer, dehydrated in ethanol and put through the criHcal point dryer, and coated with plaHnum in the spuJer coater.
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Depth of field – Tangled clump of hair. Specimen spuJer coated with plaHnum.
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Backsca8er (Secondary image) – Specimen was leL uncoated.
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Backsca8er (BEI composi$on image) – Specimen was leL uncoated.
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Low kV uncoated sample – Specimen was leL uncoated and was air dried.
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High magnifica$on – Specimen was air dried and was coated with gold palladium in the spuJer coater. Taken at 15 kV.
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Stereo image – Specimen was air dried and was coated with gold palladium in the spuJer coater. Stereo image taken using rotaHon of +8 and 0 at Hlt of 50 degrees. Taken at 10 kV.
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Cryofracture – Specimen was dried in ethanol and freeze fractured using liquid nitrogen, then put through the criHcal point dryer. It was then coated in plaHnum in the spuJer coater.
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Aesthe$c – Specimen fixed in 2.5% glut, rinsed in sodium cacodylate buffer, dehydrated in ethanol and put through the criHcal point dryer, and coated with plaHnum in the spuJer coater.
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Aesthe$c – Specimen fixed in 2.5% glut, rinsed in sodium cacodylate buffer, dehydrated in ethanol and put through the criHcal point dryer, and coated with plaHnum in the spuJer coater.
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Part 2
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Biological 1 – Hyphae on the surface of a mushroom cap. Specimen air dried and spuJer coated with gold palladium. Taken at 10 kV.
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Biological 2 – Spores and underlying hymenium of a gilled mushroom. Specimen air dried and spuJer coated with gold palladium. Taken at 10 kV, x1600.
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Biological 3 – Surface of a broken toothpick. Specimen spuJer coated with plaHnum.
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Biological 3 – Tangled clump of hair. Specimen spuJer coated with plaHnum.
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Non-‐biological 1 – Cubic crystalline structures on a chunk of rock salt. Specimen spuJer coated with plaHnum.
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Non-‐biological 2 – Crystalline structures on a chunk of rock salt. Specimen spuJer coated with plaHnum.
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Non-‐biological 3 – BEI composiHon image of the metal part of a pushpin, showing indentaHons. Darker blotches are dust with lower atomic number. Specimen was leL uncoated.
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Non-‐biological 4 – BEI composiHon image of the point of a pushpin. Darker blotches are dust with lower atomic number. Specimen was leL uncoated.