Cng ngh sn xut enzym L Ngc Khnh

Nu c u nhc im ca cc ngun nguyn liu trong SX enzym

Trnh by c cc pp ln men VSV trong SX enzym

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MC TIU DY HC

1. I CNG V SX ENZYM

nh ngha enzym C trong t bo Cht xc tc sinh hc:

cng xc tc mnh c hiu

Bn cht protein

I CNG V SX ENZYM

Cu trc Enzym n gin: protein (~100/3.000) Enzym phc tp:

protein (apoenzym) khng protein (coenzym)

Cc L - - amino acid kt hp bng lin kt peptit

Trng lng t 12.000 n 1.000.000 dalton

I CNG V SX ENZYM

Tnh cht C hot tnh xc tc mnh, c hiu Trng lng phn t ln kh qua mng

t bo Lng tnh, tan trong nc, mui long,

glycerin, dd hu c phn cc p/p tch chit, tinh ch

Khng bn nhit cao, acid, kim mnh, mui kim loi nng

I CNG V SX ENZYM

Ngun nguyn liu: khng tng hp ha hc, ch yu t ng vt, thc vt, vi sinh vt

PP sn xut Tch chit t ng vt, thc vt Sinh tng hp t vi sinh vt

Tch chit v tinh ch Tch chit t ng, thc vt: gii phng

enzym tch chit v tinh ch da vo t/c Sinh tng hp t VSV: enzym ni bo hay

ngoi bo, tch chit v tinh ch da vo t/c

I CNG V SX ENZYM

ng dng Thuc: c > 60 E c s dng Loi cc mu ng: streptokinase, urokinase, nattokinase Sch vt thng: proteinase, trypsin, chymotrypsin, collagenase, papain Tr bnh bch cu: L - asparaginase H tr tiu ho: amylase, pepsin, pancrease, bromelain, papain Tr nhim khun: lysozyme

I CNG V SX ENZYM

ng dng Thuc:

u im ca vic s dng thuc cha E - t phn ng ph c hi - c tnh c hiu cao Hn ch ca vic s dng thuc cha E - Khi lng p/t ln kh qua mng t bo - D bin tnh (tO, pH acid..) ng tiu ho - C th b bt hot bi h dch ca c th - D gy phn ng to khng th khi s dng lp li nhiu ln - Gi thnh cao

I CNG V SX ENZYM

MC TIU 1

2. CC NGUN NGUYN LIU SN XUT ENZYM

Hu nh khng th SX E bng pp ha hc ngun ng/liu sinh hc

Mi sinh vt u c coi l ng/liu SX E Phn b E khng ng u gia cc loi, t bo

m v c quan khc nhau Kh nng tng hp E gia cc t bo khc loi

khng ging nhau Hm lng E trong mt m hoc mt c quan

ph thuc vo: - giai on sinh trng v pht trin - trng thi sinh l ca t bo - cc yu t bn ngoi

NGUN NGUYN LIU

Ngun nguyn liu ch yu t: M v c quan ng vt M v c quan thc vt T bo VSV (VK, nm mc, nm men)

NGUN NGUYN LIU

c th a bo ch mt s b phn c s dng SX E: cc tuyn dch, ni tng

SP E ch yu l cc protease Pepsin: t mng nhy d dy Pancreatin: t tu ln, b Tripsin: t dch tu b (SX cc chymotripsin) Lipase, lysozyme

NGUN NGUYN LIU NG VT

u im: - hot tnh v tnh n nh cao Nhc im: - ngun nguyn liu khng n nh - khng ci to c - t (t/g lu, h/sut thp, thc phm) - yu t ri ro cao (dch bnh, thin tai)

NGUN NGUYN LIU NG VT

L ngun SX Enzym t rt lu i Nhm E c SX l amylase, protease Amylase: t thc i mch ny mm Papain: t nha m u Bromelin: t chi v li da Ficin: t sung, tr k sinh trng ng rut

NGUN NGUYN LIU THC VT

u im: - hot tnh mnh, n nh - r hn ngun ng vt Nhc im: - ngun nguyn liu khng n nh - khng ci to c - t (t/g lu, hm lng thp, lng thc) - yu t ri ro cao (dch bnh, thin tai)

NGUN NGUYN LIU THC VT

Cng ngh Enzym VSV pht trin mnh cui TK 20

Ngun E VSV dn dn thay th E ng vt v thc vt (chim 90% th trng)

Hu ht cc E cng nghip c SX t VSV: amylase, protease, pectinase, penicilinacylase

NGUN NGUYN LIU VI SINH VT

u im: VSV l nhng c th n bo, tc sinh

sn nhanh nhiu SK, thun li hn ng thc vt

H E VSV a dng (cellulose, racemase, nitrogenase ch c VSV)

T l E trong t bo ln SX d, h/sut cao

Hot tnh cao, n nh trong khong to v pH rng

NGUN NGUYN LIU VI SINH VT

u im: VSV c th cho tt c cc loi E (mi thc

vt hay ng vt ch cho 1 loi E) C th iu khin sinh E theo mun, km

hm sinh enzym khng mong mun (sd cht cm ng) Asp. niger + MT protein protease + MT pectin pectinase

NGUN NGUYN LIU VI SINH VT

u im: Ng/liu d kim, r tin SX khng cn cc t/b chng n mn SP khng cn tinh khit cao Khng ph thuc thi tit, mi trng

NGUN NGUYN LIU VI SINH VT

MC TIU 2

3. CC PP LN MEN SN XUT ENZYM T VSV

Chng ging: vi khun, nm men, nm mc, x khun c im VSV, enzym ca VSV sinh ra

iu kin ln men: tO

pH cp kh thi gian (da vo c im VSV, c im sn phm)

PP SN XUT ENZYM T VSV

Mi trng dinh dng: Ngun C: fructose, saccharose, glucose... Ngun N: bt u, pepton - pectinase: pectin, lactose - cellulase: b ma, mn ca, li ng... Cht cm ng sinh sn phm mong mun: - amylase: tinh bt, dextrin, maltose... - proteinase: casein Cc t/p khc: vi lng, iu chnh pH, ph

bt

PP SN XUT ENZYM T VSV

P/php ln men b mt (xp) Cm m, cm go, bt u, bt ng B sung tru, mn ca tng xp (

PP SN XUT ENZYM T VSV

P/php ln men chm Ng/liu: tinh bt, nit, khong H ho, kh trng (30ph/1atm) Nhit : 28 30OC pH: ty thuc vo VSV (thng l trung

tnh) Khuy trn, cp kh, lm mt Thi gian: 48h 96h

PP SN XUT ENZYM T VSV

u im ca ln men b mt Nng enzym cao hn ln men chm D sy kh v t hao tn hot tnh E Ch phm th d vn chuyn Thit b n gin, tn t nng lng C th x l cc b khi b nhim

PP SN XUT ENZYM T VSV

Chit E C th sd enzym th: sn xut ru hoc enzym tinh khit: y dc, thc phm, CN nh E ngoi bo: loi sinh khi E ni bo: Ph v t bo PP vt l: nhit, nghin, siu m PP ha hc: acid, kim PP sinh hc (enzym): lysozyme, cellulase Lc ly dch cha E

PP SN XUT ENZYM T VSV

Chit E E th: sy kh n 8 12% E tinh khit: - chit bng nc ct, nc mui (NaCl, (NH4)2SO4); dung mi hu c (cn, aceton) 28 - 30OC - h xung 10OC, c chn khng E tinh khit

PP SN XUT ENZYM T VSV

Tinh ch kt ta enzym trong dung mi hu c

(ethanol, isopropanol, aceton) kt ta enzym trong dd mui trung tnh sng phn t in di hp th chn lc sc k i lc siu ly tm

PP SN XUT ENZYM T VSV

Xin cm n!

Cng ngh sn xut enzymMC TIU DY HCSlide Number 6I CNG V SX ENZYMI CNG V SX ENZYMI CNG V SX ENZYMI CNG V SX ENZYMI CNG V SX ENZYMI CNG V SX ENZYMMC TIU 1NGUN NGUYN LIUNGUN NGUYN LIUNGUN NGUYN LIU NG VTNGUN NGUYN LIU NG VTNGUN NGUYN LIU THC VTNGUN NGUYN LIU THC VTNGUN NGUYN LIU VI SINH VTNGUN NGUYN LIU VI SINH VTNGUN NGUYN LIU VI SINH VTNGUN NGUYN LIU VI SINH VTMC TIU 2PP SN XUT ENZYM T VSVPP SN XUT ENZYM T VSVPP SN XUT ENZYM T VSVPP SN XUT ENZYM T VSVPP SN XUT ENZYM T VSVPP SN XUT ENZYM T VSVPP SN XUT ENZYM T VSVPP SN XUT ENZYM T VSVPP SN XUT ENZYM T VSVXin cm n!