ChronosBH—ISS technology combined with Becker & Hickl electronics for precise TCSPC measurements using laser diodes and LEDs.ChronosBH is a time-domain fluorometer with picosecond resolution. Its optical design and automatic instrument control are state-of-the-art for time-resolved fluorometers.

User-Friendly SoftwareChronosBH includes Vinci- Multidimensional Fluorescence Spectroscopy,a powerful software package that provides several ready-to-use routines for reliable, user-friendly acquisition of complex fluorescence data:

Spectra (excitation, emission, synchronous, time-resolved andpolarization)Measurements at fixed wavelengths (intensity and polarization)Measurement of kinetics dataTime-resolved measurements (lifetimes and rotational correlation times)

Designed for Steady-State & Time-Resolved ApplicationsSteady-State Measurements

Intensity measurements at fixed wavelengthsPolarization (anisotropy) measurements at fixed wavelengths

Time-Resolved Measurements

Single- and multi-exponential decaysAnisotropy decaysTime-resolved kineticsTime-resolved spectraFRET

Key Features

Time-domain lifetime measurements with picosecond resolution

Lifetime measurement range from milliseconds to picoseconds

Flexible instrument configuration with a variety of light sources

T-format and parallel beam optical design for fast and precise polarization measurements

Full automation of instrument components including: cuvette holder, polarizers, shutters, filterwheel, monochromators and stirrers

PC-controlled integration of temperature bath, titrator, stopped-flow apparatus and pressure pump

Powered by Vinci - Multidimensional Fluorescence Spectroscopy.

Vinci, the Complete Software Solution forSteady-State and Time-Resolved Applications

A powerful and flexible multidimensional fluorescence spectroscopy software with ready-to-use routines for data acquisition and analysis.

chronosBHInstrument AutomationChronosBH is the instrument of choice for reliable-time resolved dataacquisition using laser diodes and LEDs. All hardware components,including external devices, are automated and PC-controlled.

User-friendly AcquisitionA sequence of measurements is acquired through a one-time setup of the experiment file, allowing for the automatic acquisition of multiple data sets.

Personalized LoginWith its unique system memory design, Vinci allows user-specific access. In multi-user environments each user may perform measure-ments with a personalized instrument configuration.

Data Analysis

Decay TimesDecay time analysis is performed on multiple data sets using various models including multi-exponential, non-exponential and lifetime distributions.

Rotational Correlation TimesAnisotropy decay data analysis of up to three species using modelsfor isotropic, anisotropic and hindered rotators.

Phasor (polar) plot analysisA powerful graphical approach to fluorescence decay data analysisused to quantify individual components of a mixture, FRET processesand excited states reactions.

Light Sources: Laser diodes (405, 436, 473, 635, 690, 780 and 830 nm), Ti:Sapphire, super continuum and other pulsed lasers.

Focusing & Collection Geometry: Parallel beamdesign for precise polarization measurements

Polarizers: UV grade Glan-Thompson with L/A=2.0

Detectors: PMTs, MCPs, APDs

Wavelength Range: 160 – 850 nm (MCP),185 – 850 (PMT)

TCSPC Modules:• Electrical Time Resolution down to

8 ps FWHM/5 ps rms

• Minimum Time Channel Width 820 fs

• Total useful count rate up to 4 MHz

• Measurement times down to 1 ms

Lifetime Measurement Range: 10-11 to 10-2 sec

OS Requirements: Windows 7

Power Requirements: Universal power input of110-240 V, 50/60 Hz, 400 VAC

Dimensions: 540 mm (L) x 400 mm (W) x 330 mm (H)

Weight: 26 kg

Information & specifications are subject to change without notice.

For more information about ChronosBH,including a complete list of accessories,call (217) 359-8681 or visit www.iss.com.

1602 newton drivechampaign, illinois 61822, usa217-359-8681

©2014 iss inc. all rights reserved chronosbh-1211

Data Display & Export

2D and 3D display with user-defined colors and fonts

3D surface rotation and in/out zooming

3D display of user-defined functions

Cursor identification of XY spectra coordinates

Time-resolved spectra display as 3D and center of gravity plots

Export to gif, png, jpeg, bitmap and metafile formats

Data are generated and exported in ASCII format

ChronosDFD includes Vinci- Multidimensional Fluorescence Spectroscopy, a powerful software package that provides several ready-to -use routines for reliable, user friendly acquisition of complex fluorescence data:

Chronos DFD - ISS Digital Frequency Domain technology for Lifetime measurements in complex decays in less than 1 second.

The ChronosDFD uses the unique digital frequency domain technology. The light sources (laser dioded and LEDs) are modulated using square pulses of about 2 nanosecond duration with a repetition rate selected by the user and in the range from 0,05 Hz. to 80 MHz. The frequency signal contains the fundamental frequency at the repetition rate and its harmonics for up to thirty; that is, when the fundamental frequency is, for instance 10 MHz, harmonics at 20,30,40 and all the way up to 300 MHz are generated. The detector photons are separated into phase bins depending upon their individual phase shift and demodulation until a phase histogram is built; from the the histogram the decay times are determined.

ChronosDFD is capable of measuring decay times of fluorescence and rotational correlation times of complex fluorescence mixtures in 1 second with an accuracy of a few picoseconds. The novel instrument opens a number of applications until now hindered by the duration of the acquisition: the capability of measuring fluorescence decay in a short time makes the instrument usable for measuring fast kinetics processes; the decay times of mixtures in a stopped flow apparatus and in chromatography; and lifetimes in samples where the exposure to light has to be minimized in order to avoid photobleaching.

Flexible instrument configuration with a variety of light sources (laser diodes, LEDs, Supercontinuum laser and Ti Sapphire laser)

Second to picosecond lifetime measurement capabilities using the same hardware

Complete lifetime scans in one second on routine samples with proprietary Digital Frequency domain technology

A compact footprint and short optical pathlenght for maximum sensitivity and efficient light coupling into the sample

T-format and parallel beam optical design for fast and precise polarization measurements

Full automation of instrument components including: cuvette holder, polarizers, shutters, filter wheel, monochromators and stirrers

PC-controlled integration of temperature bath, titrator, stopped-flow apparatus & pressure pump

User selectable data acquisition display: time-domain or frequency-domain

Spectra (excitation, emission, synchronous, time-resolved and polarization)Measurements at fixed wavelenghts (intensity and polarization)Measurements of kineticsTime-resolved measurements (lifetimes and rotational correlation times)

How does it work?

User friendly Software

Key Features

Designed for Steady-State & Time -Resolved Applications

Intensity measurements at fixed wavelengthsPolarization (anisotropy) measurements at fixed wavelengthsSlow and fast kineticsDual wavelength emission-ratiometric measurements

Frequency responses of single and multi exponential decaysAnisotropy decaysPhase and modulation resolved kineticsPhase and modulation resolved spectraTime resolved spectraFRET

schronosDFD

Instrument AutomationChronosDFD is the instrument of choice for reliable time-resolved data acquisition using laser diodes and LEDs. All hardware components, including external devices, are automated and PC-controlled.

Instrument Diagnostic and Noise DetectionVinci includes routines for analyzing the instrument’s performance, allowing the user to monitor data acquisition and noise level during the entire measurement. If sample saturation occurs the signal level is adjusted accordingly.

User-friendly AcquisitionA sequence of measurements is acquired through a one-time setup of the experiment file, allowing for the automatic acquisition of multiple data sets.

Personalized Log-onWith its unique system memory design, Vinci allows user-specificaccess. In multi-user environments each user may perform measure-ments with a personalized instrument configuration.

Light Sources (Internally modulated or pulsed):

Information & specifications are subject to change without notice.

For more information and a complete list of accessories for ChronosDFD, please visit www.iss.com

Data Analysis

Decay TimesDecay time analysis is performed on multiple data sets using various models including multi-exponential, non-exponential and lifetime distributions.

Rotational Correlation TimesAnisotropy decay data analysis of up to three species using models for isotropic, anisotropic and hindered rotators.

Phase- and Modulation-Resolved SpectraVinci also includes routines for the separation of up to three

components in phase- and modulation-resolved spectra.

Data Display & Export

2D and 3D display with user-defined colors and fonts3D surface rotation and in/out zooming3D display of user-defined functionsCursor identification of XY spectra coordinatesTime-resolved spectra display as 3D and center of gravity plotsExport to gif, png, jpeg, bitmap and metafile formatsData are generated and exported in ASCII format

Phasor (polar) plot analysisA powerful graphical approach to fluorescence decay data analysisused to quantify individual components of a mixture, FRET processes and excited states reactions.

1602 newton drivechampaign, illinois 61822, usa217-359-8681 ©2017 iss inc. all rights reserved ds.chronos.1211

Focusing & Collection Geometry: Parallel beam design for precise polarization measurements

Polarizers: UV grade Glan-Thompson with L/A=2.0

Light Detectors: PMT, hybrid PMT, MCP, APD

Lifetime Measurements Range: 10-12 to 10-2 sec

OS Requirements: Windows 10

Power Requirements: Universal power input of110-240 V, 50/60 Hz, 400 VAC

Dimensions: 540 mm (L) x 400 mm (W) x 330 mm (H)

Weight: 26 kg

Wavelenght Range: From 200 nm to 1700 nm(detector-dependent)

Max Counts Range: Up to 13 million counts/s(hybrid detectors)

Laser diodes: 370, 405, 436, 473, 635,690, 780 and 830 nm

LEDs: (265, 280, 300, 335, 355, 370, 460, 480, 500 and 520 nm)

Optional: Xenon Arc Lamp, Continuous-wave (CW), Super continuum laser and Ti:Sapphire laser

The K2 family of instruments offers a solid,well-proven approach to fluorescence andphosphorescence instrumentation even forthe most demanding research applications.

K2 is a frequency domain fluorometer with picosecond resolution. Its optical design and automatic instrument control are state-of-the-art for steady-state and time-resolved fluorescence measurements.

Designed for Steady-State & Time-Resolved ApplicationsSteady-State Measurements

Corrected excitation and emission spectraPolarization (anisotropy) measurementsSlow and fast kineticsSynchronous luminescence spectraIntensity measurements at fixed wavelengthsDual-wavelength excitation (emission) ratiometric measurements

Time-Resolved Measurements

Frequency responses of single- and multi-exponential decaysAnisotropy decaysPhase- and modulation-resolved kineticsPhase- and modulation-resolved spectraTime-resolved spectraFRET

Key Features

Millisecond to picosecond lifetime measurement capabilities

Complete lifetime scans in less than one minute on routine samples with proprietary FastScan™ technology

A compact footprint and a short optical path-length for maximum sensitivity and efficient light coupling into the sample

T-format and parallel beam design for fast and precise polarization measurements

Full automation of instrument components (cuvette holder, polarizers, shutters, filterwheel, monochromators and stirrers)

PC-controlled integration of temperature bath, titrator, stopped-flow apparatus and pressure pump

Fast kinetic measurements with one millisecond resolution in photon counting mode

80 MHz pre-amplifier discriminators delivering linearity up to over eight million counts per second on each channel

Flexible instrument configuration

Upgradable with ozone free lamp, laser diodes, continuous- wave (CW) and TI:Sapphire lasers

Vinci, the Complete Software Solution forSteady-State and Time-Resolved Applications

A powerful and flexible multidimensional fluorescence spectroscopy software with ready-to-use routines for data acquisition and analysis.

User-Friendly SoftwareK2 includes Vinci – Multidimensional Fluorescence Spectroscopy, a powerful software package that provides several ready-to-use routines for reliable, user-friendly acquisition of complex fluorescence data:

Spectra (excitation, emission, synchronous, time-resolved and polarization)Measurements at fixed wavelengths (intensity and polarization)Slow and fast kineticsTime-resolved measurements (lifetimes and rotational correlation times)

sk2

Instrument AutomationK2 is the instrument of choice for steady-state and time-resolvedresearch applications. All hardware components, including externaldevices, are automated and PC-controlled.

Adaptive MeasurementsThe operator has the option to choose between two Signal QualityParameters: Standard Deviation and Time. Upon reaching either a pre-defined standard deviation or measurement time, the K2 auto-matically continues the routine to the next data point.

Instrument Diagnostic and Noise DetectionVinci includes routines for analyzing the instrument’s performance, allowing the user to monitor data acquisition and noise level during the entire measurement. If sample saturation occurs the signal level is adjusted accordingly.

User-friendly AcquisitionA sequence of measurements is acquired through a one-time setup of the experiment file, allowing for the automatic acquisition of multiple data sets.

Personalized Log-onWith its unique system memory design, Vinci allows user-specificaccess. In multi-user environments each user may perform measure-ments with a personalized instrument configuration.

Light Source: 300 W xenon arc lamp with 45 mW/nmbrightness at 275 nm.Optional: Ozone free lamp, laser diodes, continuous-wave (CW) and Ti:Sapphire lasers

Monochromators: Highly efficient single concave holographic grating monochromators

• Fixed slits ranging from 0.4 to 32 nmbandpass

• Wavelength range: 200 nm to 1200*nm(*depending on selected grating)

• Stray light: 10-5 outside the band pass ofthe 632.8 nm HeNe laser line

• Wavelength accuracy: ±0.2 nm• Wavelength reproducibility: ±0.25 nm• Slew rate: 160 nm/s

Optional: Double-grating monochromator

Focusing & Collection Geometry: Parallel beam design for precise polarization measurements

Polarizers: UV grade Glan-Thompson with L/A=2.0

Detectors: Selected side-on photomultiplier tubes. Optional: Cooled PMT, microchannel plate detector

Detection Modes: Fast analog and photon counting electronics

Light Modulator: Double-crystal Pockels cell

Frequency Response: 90 Hz – 6 GHz

Cross-Correlation Frequency: User-selected up to 10 KHz

Filter Bandwidth: 0.05 Hz at 40 Hz

Sensitivity: S/N ratio greater than 5000:1 on the Raman spectrum of water with photon counting and cooled PMT

Lifetime Measurements Range: 10-12 to 10-2 sec

OS Requirements: Windows XP, Windows 7

Power Requirements: Universal power input of110-240 V, 50/60 Hz, 400 VAC

Dimensions: 885 mm (L) x 600 mm (W) x 330 mm (H)

Weight: 45 kg

Information & specifications are subject to change without notice.

For more information and a complete list of accessories for k2, please visit www.iss.com

Data Analysis

Decay TimesDecay time analysis is performed on multiple data sets using various models including multi-exponential, non-exponential and lifetime distributions.

Rotational Correlation TimesAnisotropy decay data analysis of up to three species using models for isotropic, anisotropic and hindered rotators.

Phase- and Modulation-Resolved SpectraVinci also includes routines for the separation of up to three components in phase- and modulation-resolved spectra.

Data Display & Export

2D and 3D display with user-defined colors and fonts3D surface rotation and in/out zooming3D display of user-defined functionsCursor identification of XY spectra coordinatesTime-resolved spectra display as 3D and center of gravity plotsExport to gif, png, jpeg, bitmap and metafile formatsData are generated and exported in ASCII format

Phasor (polar) plot analysisA powerful graphical approach to fluorescence decay data analysis used to quantify individual components of a mixture, FRET processes and excitedstates reactions.

1602 newton drivechampaign, illinois 61822, usa217-359-8681

©2012 iss inc. all rights reserved ds.k2.1211

PC1 is a compact benchtop photon counting spectrofluorimeter for research applications demanding the highest sensitivity.

PC1 optical design and automatic instrument control are state-of-the-art for steady-state fluorescence measurements.

Fully Automated for Steady-State ApplicationsCorrected excitation and emission spectraPolarization (anisotropy) measurementsSlow and fast kineticsSynchronous luminescence spectraIntensity measurements at fixed wavelengthsDual-wavelength excitation (emission) ratiometric measurements

One-dimensional and multi-dimensional data acquisition with user defined protocols. The user can select any of the following experimental combinations:

Key FeaturesA compact footprint and short optical pathlength for maximum sensitivity and efficient light cou-pling into the sample

T-format and parallel beam design for fast and precise polarization measurements

Full automation of instrument components including: cuvette holder, polarizers, shutters, filterwheel, monochromators and stirrers

PC-controlled integration of temperature bath, titrator, stopped-flow apparatus and pressure pump

Fast kinetic measurements with one millisecond resolution in photon counting mode

80 MHz pre-amplifier discriminators delivering linearity up to eight million counts per second on each channel

Upgradable for time-resolved measurements with picosecond resolution

vsFluorescence Intensity

Polarization/Anisotropy

Ratiometric Measurements

Excitation (Emission) Wavelength and/or any of the following:Emission (Excitation) Wavelength

Sample Concentration, pH, Pressure &

Temperature

Vinci, the Complete Software Solution forSteady-State and Time-Resolved Applications

A powerful and flexible multidimensional fluorescence spectroscopy software with ready-to-use routines for data acquisition and analysis.

User-Friendly SoftwarePC1 includes Vinci – Multidimensional Fluorescence Spectroscopy, a powerful software package that provides several ready-to-use routines for reliable, user-friendly acquisition of complex fluorescence data:

Spectra (excitation, emission, synchronous, time-resolved and polarization)Measurements at fixed wavelengths (intensity and polarization)Measurement of kineticsTime-resolved measurements (lifetimes and rotational correlation times)

pc1Instrument AutomationPC1 is the instrument of choice for steady-state research applications. All hardware components, including external devices, are automated and PC-controlled.

Adaptive MeasurementsThe operator has the option to choose between two Signal Quality Parameters: Standard Deviation and Time. Upon reaching either a pre-defined standard deviation or measurement time, the PC1 auto-matically continues the routine to the next data point.

Instrument Diagnostic and Noise DetectionVinci includes routines for analyzing the instrument’s performance, allowing the user to monitor data acquisition and noise level during the entire measurement. If sample saturation occurs the signal level is adjusted accordingly.

User-friendly AcquisitionA sequence of measurements is acquired through a one-time setup of the experiment file, allowing for the automatic acquisition of multiple data sets.

Personalized Log-onWith its unique system memory design, Vinci allows user-specific access. In multi-user environments each user may perform measurements with a personalized instrument configuration.

Light Source: 300 W xenon arc lamp with 45 mW/nm brightness at 275 nm.Optional: Ozone free lamp, lasers, laser diodes and LEDs

Monochromators: Highly efficient single concave holographic grating monochromators

• Fixed slits ranging from 0.4 nm to 32 nm bandpass

• Wavelength range: 200 nm to 1200*nm(*depending on selected grating)

• Stray light: 10-5 outside the band pass ofthe 632.8 nm HeNe laser line

• Wavelength accuracy: ±0.2 nm• Wavelength reproducibility: ±0.25 nm• Slew rate: 160 nm/s

Optional: Double-grating monochromator

Focusing & Collection Geometry: Parallel beam design for precise polarization measurements

Polarizers: UV grade Glan-Thompson with L/A=2.0

Detectors: Side-on photomultiplier tubesOptional: Cooled PMT, microchannel plate detector

Detection Modes: 80 MHz pre-amplifier discrimina-tors for fast photon counting acquisition on three independent channels

Sensitivity: S/N ratio greater than 5000:1 on theRaman spectrum of water with photon counting and cooled PMT

Dynamic Range: Linear up to eight million counts per second

OS Requirements: Windows XP, Windows 7

Power Requirements: Universal power input of 110-240 V, 50/60 Hz, 400 VAC

Dimensions: 885 mm (L) x 600 mm (W) x 330 mm (H)

Weight: 43 kg

Information & specifications are subject to change without notice.

For more information and a complete list of accessories for PC1, please visit www.iss.com.

Data Analysis

User-Defined Data File OperationsReformat

Normalization

Smoothing (average, Savitzky-Golay)

Derivative (nth-order)

Integration (area, average and standard deviation)

Convolution with a user-defined function

Spectral correction

Conversion to wave numbers

Data Display & Export

2D and 3D display with user-defined colors and fonts

3D surface rotation and in/out zooming

3D display of user-defined functions

Cursor identification of XY spectra coordinates

Export to gif, png, jpeg, bitmap and metafile formats

1602 newton drivechampaign, illinois 61822, usa217-359-8681

©2012 iss inc. all rights reserved ds.pc1.1211

QUANTA Measuring particles at low concentrationThe QUANTA system is an instrument designed for the accurate detection and rapid counting of particles in solution at very low concentrations, (102 ~ 105 particles/mL for 1 µm diameter beads).

Applications Quantifying protein aggregation

Determining the concentration of nanoparticles

Measuring the concentration of viruses, bacteriophage and their aggregates in a physiological

fluid and any instance in which it is relevant to determine the presence of particles at very low numbers.

Principle of Operations

TM

Particle Counter

www.iss.com

QUANTA is designed to measure individual particle fluorescence. In order for QUANTA to count particles in a given

solution, either the particles must themselves be fluorescent or they must be tagged using a suitable fluorophore.

In both cases , the wavelength of the applied laser light must be matched to the excitation wavelength of the fluorophore

in use. Laser modules are available in a variety of wavelengths.

The QUANTA system is designed to measure solutions in glass vials secured within the sample stage via a tight-fit base

with optional hex screw fastening and a spring pressured centering cap which is connected to the top of the sample

stage. Both the tight-fit base and centering cap are mounted using radial ball-bearings, providing for unrestricted

rotational motion. The vial undergoes a rotational motion around the axis as well as a vertical motion along the axis.

The combination of these two motions allows for the fixed confocal volume determined by the laser light inside the

cuvette to explore a wider volume space in search of the sparse particles.

For more information call (217) 359-8681 or visit our website at www.iss.comCopyright ©2017 ISS, Inc. All Rights Reserved

1602 Newton DriveChampaign, Illinois 61822 USATelephone: (217) 359-8681iss@iss.com

Objective X20 standard, w.d.=6 mm

Detector PMT Model H9305-04 by Hamamatsu

Light Sources 375, 405, 440, 473, 488, 514, 532, 635 nm

Computer Portable computer, Windows 7 OS, 3 GHz, 4 GB RAM, 500 GB HD

Dimensions (without the computer) 40(D) x 53(W) x 19(H) cm

Weight 20 Kg

Electrical requirements 110-240 V, 50-60 Hz

Software The software includes three components:

• The control of the instrument automated features (rotational and axial movement of the vial;

shutters; positioning of the objective)

• Data acquisition

• Data analysis using a pattern recognition (PR) software that separates the counts due to the particles from

the background noise and allows for a reconstruction of the total number of particles present in the observation

volume.

QUANTA is covered by US Patent no. 7,528,384; US Patent no. 7,973,294; EP 1846751. Other Patents are pending.

Time threshold analysis split charts view . Zoom in/out and threshold settings for every recorded channel. Experiment button unload analysis module and return to experiment module.

Time threshold analysis single charts view . Zoom in/out and threshold settings for every recorded channel. Experiment button unload analysis module and return to experi-ment module.

The measurement capabilities of the Alba v5 include:

User-Friendly Software

Key Features

Alba is a laser scanning microscope that incorporates several measurement modalities for experimental quantitative biology and material science applications requiring the single molecule detection sensitivity.

FRET and FLIM imagesPolarization and ratiometric imagesKineticsTime-lapse recordingRICS (Raster Scan Image Correlation Spectroscopy)N&B (Number & Brightness)FCS and FCCS (Auto- and cross-correlation)PCHs (Photon Counting Histograms)Fluorescence Polarization FCSScanning FCSParticle Tracking

Digital Frequency-domain or

Time-domain (TCSPC)

Four-channel instrument

Single- and multi-photon excitation

Computer-controlled optimization of

optical elements

Imaging with galvo scanning mirrors or

piezo-controlled XYZ stage

Flexibility and Versatility

Alba includes Vista Vision - a comprehensive ,user friendly software package for acquiringFCS, PCH, FLCS, FLIM, FRET and RICS data.

albac o n f o c a l s p e c t r o s c o p y & i m a g i n g w o r k s t a t i o n

For more information and a complete list of accessories for Alba v5please visit www.iss.com

Image Acquisition

Data Acquisition & Analysis

Vista Vision – FCS and Confocal Imaging Microscopy SoftwareAlba features Vista Vision, a comprehensive, user-friendly software package for the acquisition and analysis of FLIM, FRET, FCS, FCCS and RICS data.

Image Acquisition (Raster Scan)Vista Vision offers the user the flexibility to choose between the following image acquisition parameters:

Pixels number: user selectable from 2 to 8192Max line frequency: 4 KHz (on 20 points)Min line frequency: 0.01 HzMax frame rate 512x512: 2 secMax frame rate 256x256: 0.4 secBeam parkPanningField rotation: 200o opticalField diameter: 18 mm

Scan ModesVista Vision provides several options for kinetic studies (t, Xt, XYt, XZ, XYZ and XZt), and for optical sectioning (XZ, XYZ) of specimens.

Input/Output2 channels input5 channels output

Image FormatsExport to ImageJ, MetaMorphPlots can be saved and exported to GIF, TIFF, JPEG, PNG,Bitmap and Metafile formats

Data Acquisition ModesAlba acquires data in either time mode (photons are counted during fixed, user-defined time intervals), or photon mode (time delay between photons is used to build histograms).

FFS Data AnalysisVista Vision utilizes the following statistical functions for data analysis:Auto-correlation function, Cross-correlation functionPhoton Counting Histogram (PCH)A custom function can be used as a model

FLIM Data AnalysisMinimization TechniquePhasor plots

Light Sources:• Up to six CW or pulsed single photon lasershoused in a laser launcher with laser intensity,shutters and single-mode fiber optic output.Wavelengths: 405, 440, 473, 488, 532, 543, 594,635 and 690 nm• Multi-photon excitation with computer-controlledbeam expander, laser intensity and shutters.

Microscope: Inverted or upright microscope (Nikon,Olympus, Zeiss, Leica)

Optics:Objectives:• Air objectives with 20X, 40X, 60X magnification and 1.5-8.1 mm working distances• Water objectives with 60X magnification and 0.22 mm working distance• Oil objectives with 60X magnification, 1.4 NA and 0.21 mm working distance

Dichroic Filters:• For single-photon excitation: 1-, 2-, 3-4 band filters• For multi-photon excitation Polarizer:• Cube beam splitter, wavelength range:450-1100 nm; extinction ratio: 10,000:1at +/- 3 degrees

Confocal Pinholes:• Separate pinholes for each emission channel• From 12.5 µm to 144 µm

Stage: Large distance movement (100x100x10 mm),stepper motor-controlled XYZ stage

Scanning Options:• Galvanometrically-controlled mirrors• Piezo-controlled stage

Sample Holders: 8-, 96-, and 384-well plates, petri dishes and coverslips

Light Detectors: Avalanche photodiodes (APDs)

Power Requirements : Universal power input of110-240 V, 50/60 Hz, 400 VAC

Dimensions: 538 mm (L) x 563 mm (W) x 205 mm (H)

Weight: 27 kg

Featuring:

- Single molecule level detection from 300 nm to 1050 nm

- Wide range of lifetime measurements from 100 ps to 100 ms

- Intensity and lifetime images simultaneous acquisition (at a

diffraction limited resolution down to 250 nm)

- Optical sectioning for 3D imaging at 50-nm step resolution

- Scanning large samples up to 100 x 100 mm

- Integrated software (64 bit) for instrument control,

data acquisition and analysis

- Compact design with flexible customization to fit your needs and

maximize your research outcome

Materials Science Research

- Semiconductor characterization

- Solar cells

- Crystals

ISS has more than 30 years of innovation in research-grade photo luminescenceinstrumentation. PL1 is the latest affordable solution for Quantitative Imagingand Analysis. Contact us for more information at microscopy@iss.com

www.iss.com

Photoluminescence

TM

Time -Resolved Confocal MicroscopePL1

Introducing PL1, the compactinstrument for material sciences with single-molecule detection.

Applications

1400

1200

1000

800

600

400

200

0

10

8

6

4

2

0

Perovskite confocal lifetime imaging for both photoluminescence intensity and lifetime at the high spatial resolution.

Intensity Image

Lifetime Image (ns)

The PL1 Design

Routine Measurements

Intensity and Lifetime Imaging

- Photoluminescence Confocal Intensity Imaging - Photoluminescence Confocal Lifetime Imaging in X, Y, Z and t in both scanning and single-point modes Fluorescence Fluctuations Spectroscopy

- Fluorescence Correlation Spectroscopy (FCS)- Photon Counting Histogram ( PCH)- Fluorescence Lifetime Correlation Spectroscopy (FLCS)

Single Molecule Imaging - Burst Analysis- Single Molecule FRET Analysis

Quantum Dots

Phasor plots separate different lifetime species of Quantum Dots coated on the substrate, directly from the raw data. Ex. 470 nm, Em. 499-632 nm (Fluorescence), 475/35 nm (Reflection), scanning area 17.5µm x 17.5µm.

2

Measurement Examples

The PL1 is a user friendly and compact device that delivers robust and stable alignments with maximum productivi-ty and minimum downtime. The PL 1 maximizes any system’s sensitivity and is the ideal solution for material sciences with single- molecule detection. Easy to operate, even only after a brief training session.

( Courtesy of Dr. Wenjie Liu and Dr. Yaowu Hu; Purdue University; West Lafayette, IN; USA)

Perovskite Characterization

Perovskite confocal lifetime imaging for both photoluminescence intensity and lifetime at the high spatial resolution. ISS VistaVision provides both the minimization fitting algorithm and the phasor plots for lifetime analysis; for example, using the multi-image phasor analysis routine, the lifetime changes in different Perovskite samples can be immediately identified by the phasor plots of their raw data sets (no fitting required), making the lifetime analysis much easier and robust. Excitation was 488 nm; emission through a 635 nm long pass filter; scanning area 20 µm x 20 µm.

Upconversion nanoparticle confocal lifetime imaging for both photoluminescence intensity and lifetime at the high spatial resolution. Ex. 980 nm, Em. 509-552 nm, scanning area 20µm x 20µm

Nanoparticles Upconversion

3

.( Courtesy of Dr. Yan Guan, Peking University, Beijing; P.R.

China.)

( Courtesy of Dr. Xiaodan Zhang; Nan Kai University,

Tianjin, P.R. China)

For more information call (217) 359-8681 or visit our website at www.iss.comCopyright ©2017 ISS, Inc. All Rights Reserved

1602 Newton DriveChampaign, Illinois 61822 USATelephone: (217) 359-8681iss@iss.com

FastFLIM is covered by US Patent 8,330,123; other patents are pending.

Laser Sources

Microscope and Coupling

XYZ Stage scan

Frame format: Upright or Inverted research microscope

Magnification: 10X and 60X, oil immersion objective (standard); optional: from 2X to 100X

Spatial Resolution: diffraction limited

Eye Observation: bright field by 10X eyepiece with diopter adjustment, field of view: 22 mm

Imaging Modes:

-Transmission mode: HAL Kōhler illumination for bright field imaging by a CMOS camera

with options for phase contract and DIC

-

Closed-loop DC servo control

- XY axis range of travel: 100mm x 100 mm (upright), 120mm x 75mm (inverted)

- XY axis: Resolution = 22 nm, max velocity = 7 mm/sec, RMS repeatability < 700 nm

- Z axis: Resolution = 50 nm, maximum velocity = 0.6 mm/sec, repeatability = 100 nm

CW or pulsed diode laser, repetition rate up to 80MHz (tunable by software)

The laser launcher can accommodate up to 3 lasers, ranging from 375 nm to 980 nm

Each laser has its own intensity control and shutter (operated by software)

Lifetime measurement range: from 100 ms to 100 ps

Data Acquisition Mode: Photon mode, Time-tagged mode, Time-resolved time-tagged

mode (TTTR)

Dead Time: 3.125 ns, Up to 60 x 10 counts/second

Computer Connection: USB

Dark Counts < 100 / sec; TTS < 350ps;

Wavelength Range: 350-1050 nm;

Quantum Efficiency > 70% at 700nm

Software VistaVision 64bit

Scan Modes X, XY, XZ, XYZ, t, Xt, XYt, XZt, XYZt

Image format Other than the proprietary file formats which contain the imaging parameters information,

VistaVision also supports exporting the acquired data in various formats including JPEG,

TIFF, PNG, AVI, etc.

CPU(Xeon) ≥ 3.7GHz, RAM ≥ 16GB, Video RAM 1 G Hard Drive 2TB

(Including 256 GB SSD), 16x DVD+/- RW

Windows 10, 64 bit professional

Flat Panel LED Monitor 27 inches, resolution 2560x 1440

Power requirements Universal power input: 110-240 V, 50/60 Hz, 100 VAC

Data Acquisition Unit FastFLIM

Computer& Monitor

6

Image Processing& Analysis

Detectors

Visualization by various look-up tables, Contrasting, Thresholding, Smoothing, Filtering,

Scaling, Statistical analysis by histogram or line profiling, etc.

- Non-linear least square constrained deconvolution fitting routines based on the

Marquardt-Levenberg minimization algorithm in both time and frequency domains.

- The model-free phasor plots approach for instant and unbiased results

Lifetime data analysis routines

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Confocal photoluminescence imaging: laser illumination , single-point or stage scan

≥ ≥

≥ ≥

Q2 is a compact and fast multi-channel multiphoton confocal

microscope for quantitative live cell biology applications.

The instrument allows for the acquisition of confocal images, FLIM

images and all of the Fluorescence Fluctuations Spectroscopy

measurements (autocorrelation, cross-correlation, photon

counting histogram, scanning FCS, RICS, and Number &

Brightness).

The standard configuration includes two detection channels and a

multiphoton laser emitting at 780 nm although a Ti:Sapphire

laser, the supercontinuum laser and single-photon lasers can be

coupled to the instrument too. Detectors are either GaAs PMT,

hybrid PMT, or APD.

Q2 is powered by the 64-bit VistaVision software by ISS.

When equipped with the nanoimaging option it allows for the

reconstruction of images of dynamic structures with a resolution

of 20 nanometers.

Intensity and Lifetime Imaging- 1p or 2p confocal imaging in x, y, z and t

- FLIM in frequency-domain (FastFLIM) or in TCSPC

- Phosphorescence Lifetime Imaging (PLIM)

- Steady-state and time-resolved anisotropy imaging

Fluorescence Fluctuations Spectroscopy- Fluorescence Correlation Spectroscopy (FCS)

- Fluorescence Cross-Correlation Spectroscopy (FCCS)

with pulse interleaved excitations and synchronized

gating detections

- Photon Counting Histogram (PCH)

- Fluorescence Lifetime Correlation Spectroscopy (FLCS)

- Scanning FCS by orbit scanning

- Number & Brightness (N&B)

- RICS (raster imaging correlation spectroscopy)

Single Molecule Imaging- Burst Analysis

- FRET efficiency determination

- Stoichiometry determination with pulse interleaved

excitations and synchronized gating detections

3D Particle Tracking and Nanoimaging- 3D particle tracking trajectories

- Nanoimaging reconstruction with 20 nm resolution

We have entered the era of quantitative live cell biology; the dynamics of single proteins within the cell are measured and new insights about the cellular environment and inner machinery are revealed.

Laser Scanning ConfocalNanoscope