Case_2-2004
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Immunology Cases 2004 Case 2 A three year-old child developed fever, generalized lymphadenopathy 1 and a peripheral blood WBC of 100,000. 2 Eighty percent of the peripheral blood white cells were lymphoblasts. Antibody studies to cell surface markers on the leukemic lymphoblasts failed to distinguish B- cell versus T-cell acute leukemia. 1 enlarged lymph nodes 2 100,000 cells/μl Questions for Case 2 (1) The prognosis and treatment of T-cell leukemia differs from that of B-cell acute leukemia; thus, it is important to distinguish between the two. What antibody tests are used to distinguish T-cell versus B-cell acute leukemia? (2) Southern blots were performed on DNA extracted from the patient’s peripheral blood white cells, using J-region probes for the TCRβ and Ig heavy-chain genes. Describe the patterns of the Southern blots if the cells were: (a) leukemic B-cells, or (b) leukemic T-cells. What would similar Southern blots of normal peripheral blood lymphocytes show? (3) What is the difference between leukemia and lymphoma?
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Dsecribe caso clinico que tiene ser consultado Janeway Inmmunobiology
Transcript of Case_2-2004
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Immunology Cases 2004
Case 2
A three year-old child developed fever, generalized lymphadenopathy1 and a peripheral bloodWBC of 100,000.2 Eighty percent of the peripheral blood white cells were lymphoblasts.Antibody studies to cell surface markers on the leukemic lymphoblasts failed to distinguish B-cell versus T-cell acute leukemia.
1enlarged lymph nodes2100,000 cells/l
Questions for Case 2
(1) The prognosis and treatment of T-cell leukemia differs from that of B-cell acute leukemia;thus, it is important to distinguish between the two. What antibody tests are used to distinguishT-cell versus B-cell acute leukemia?
(2) Southern blots were performed on DNA extracted from the patients peripheral blood whitecells, using J-region probes for the TCR and Ig heavy-chain genes. Describe the patterns of theSouthern blots if the cells were: (a) leukemic B-cells, or (b) leukemic T-cells. What wouldsimilar Southern blots of normal peripheral blood lymphocytes show?
(3) What is the difference between leukemia and lymphoma?
