Biosynthesis of silver and gold nanoparticles using microbial biomass

Inga Zinicovscaia

E-mail: zing@nf.jinr.ru

Department of Neutron Activation Analysis Division of Nuclear Physics

Frank Laboratory of Neutron PhysicsJoint Institute for Nuclear Research

JINR Scientific Council, September 15-16, 2011

M.V. Frontasyeva, S.S. Pavlov

Frank Laboratory of Neutron Physics , JINR, Russian Federation

T. Kalabegishvili , E. Kirkesali , I. Murusidze ,

D. Pataraya, E.N. Ginturi

Andronikashvili Institute of Physics, Tbilisi , Georgia

I. Zinicovscaia, Gh. Duca

Institute of Chemistry of the Academy of Science of Moldova, Chisinau, Moldova

An important area of research in nanotechnology deals with synthesis of nanoparticles of different chemical composition and size. There is a growing need to develop environmentally gentle nanoparticle synthesis that does not use toxic chemicals in its process.

As a result, researchers in the field of nanoparticle synthesis have turned to biological systems. It is well known that many organisms, both uni-cellular and multi-cellular, are producing inorganic materials either intra- or extra-cellularly.

Introduction

3

Advantages of biological method

tightly controlled, highly reproducible

syntheses

biocompatible particles

the avoidance of toxic surfactants or organic solvents

4

Biotechnology of Biotechnology of

silver nanoparticlessilver nanoparticles

Application of silver nanoparticles

nonlinear optics

medicine

electronics

catalysis

microelectronics

6

UV-Vis spectra recorded after one week for the reaction mixture prepared using 1mM silver nitrate and 1 g

Streptomyces glaucus 71MD

+ AgNO3 1mM =

Experiment

yellowish brown pale yellow

7

Quanta 3D FEG

Resolution 1.2 nm

Magnification 5000–150000x

Voltage 1–30 kV

Scanning electron microscope

The Netherlands’ Firm “Systems for Microscopy and Analysis” (Moscow, Russia)

8

Objects of study

BacteriaStreptomyces glaucus 71MD

p

Blue-green microalga Spirulina platensis

9

Control

SEM micrographs of Spirulina platensis cells with silver nanoparticles

1 day

27 nm

10

C O

EDAX spectrum recorded from Spirulina platensis cells after formation of silver nanoparticles 11

Control

SEM micrographs of Streptomyces glaucus 71MD cells with silver nanoparticles

12 000x50 000x150 000x

12

EDAX spectrum of Streptomyces glaucus 71MD cells after exposure to silver nitrate solution

13

Biotechnology of Biotechnology of gold nanoparticlesgold nanoparticles

Application of gold nanoparticles

Catalysis

Chemical sensing

Biosensing

Medicine

15

Experiment

+ =HAuCl4

yellow red purple

Experiment I CHAuCl4 = 1mM

Incubation time: 1 − 6d

Experiment II Incubation time = constant CHAuCl4: 10-4 − 10-2M

16

UV-Vis spectra recorded after one week for the reaction mixture prepared using 1mM hydrated gold chloride and

1 g biomass of A. globiformis 151B

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Control1 day2 days3 days5 days6 days

SEM micrographs of Spirulina platensis cells with gold nanoparticles at different incubation time

Experiment I

18

HAuCl410-4M10-3M5·10-3M

10-2M

SEM micrographs of Spirulina platensis cells with gold nanoparticles at different concentrations

Experiment II

19

EDAX spectrum of Sp. platensis cells after exposure to hydrated gold chloride solution

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Conclusions

1. Production of silver and gold nanoparticles by blue-green microalgae Spirulina platensis and bacteria Streptomyces glaucus 71MD proceeds extra-cellularly

2. SEM and EDAX were used to characterize the silver and gold nanoparticles. SEM showed formation of nanoparticles in the range of:

a) 4–25 nm for Streptomyces glaucus 71MDb) 16–200 nm for Spirulina platensis (first

experiment)c) 15 nm–7 μm for Spirulina platensis (second

experiment)

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For quantitative analysis of samples the

epithermal neutron activation analysis

(ENAA) in the radioanalytical complex

REGATA at the reactor IBR-2M will be

carried out by the end of 2011

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Elemental concentration in biomass of Streptomyces glaucus (irradiation time 8 s)

Element Energy, keV Concentration, µg/g Error, %

Ag 657.76 37 5

K 1524.58 3290 8

La 1596.21 15 14

Mn 846.75 25.0 6

Na 1368.55 381 5

Sb 564.24 1.3 15

The data were obtained by Sergey Pavlov (FLNP JINR) and Arnaud Faanhof (NECSA) in June 2011 at the reactor SAFARI-I

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Thank you for attention!Thank you for attention!