BIOMARKERS AND RESIDUES - USFWS · Biomarkers and Residues. Residues. What you measure is what is...
Transcript of BIOMARKERS AND RESIDUES - USFWS · Biomarkers and Residues. Residues. What you measure is what is...
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BIOMARKERS
AND
RESIDUES
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Chippendales®
presents
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Exposure Effects
Biomarkers and Residues
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ResiduesWhat you measure is what is left after being:
DilutedDiluted
Absorbed
Chemical breakdown
Wiped off
Excreted
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Two Residue Scenarios
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Residues• Do they relate to laboratory toxicity test values?
• Do residues measured in different matrices correlate with each other?
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Residues•When are residue measurements useful?
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Diazinon residues in grass samples from upper gastrointestinal tracts of
ducks or geese
20
0NY
Res
idue
Con
cent
ratio
n (p
pm)
Source: P. Mineau and K. R. Tucker, 2002. Improving detection of pesticide poisoning in birds.Journal of Wildlife Rehabilitation 25:4-13
40
60
ONT CA MI CT
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Carbofuran concentration fromgastrointestinal tract of raptors
100
10
1
0.1
Abuse Registered Unknown
Res
idue
Con
cent
ratio
n (p
pm)
Source: P. Mineau and K. R. Tucker, 2002. Improving detection of pesticide poisoning in birds.Journal of Wildlife Rehabilitation 25:4-13
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Azinphos-methyl residues (ppm) from skin plus feathers of brown-headed cowbirds
Exposure time ppm
0 hrs (control) <0.4 (1)
36 hrs 11.3±1.4 (8) [6.1 – 18.0]
3 days 3.1±0.3 (9)[1.5 – 4.6]
7 days 2.6±0.4 (9) [0.9 – 4.2]
Detection limits for skin plus feathers = 0.4 ppm a.i.
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Owlpellets.com
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Rodenticides
AcuteCholecalciferol
Anticoagulant
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Anticoagulants
Coumarins Indandiones
First generation
Second generation
First generation
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Anticoagulants
First generation – multiple day feeding
Second generation – single feeding toxicant
For both generations - mortality can occur up to 3 weeks after exposure.
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Hematomas
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New EPA Regulations for Rodenticides- Expect increase usage of first generation anticoagulant rodenticides
Anticoagulants
Coumarins Indandiones
First generation
Second generation
First generation
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Anticoagulant rodenticide
LD50- single dose (mg/kg)
LD50 total 5-day dose (mg/kg)
First Generation Chlorophacinone 20 0.95Diphacinone 43 1.05Warfarin 186 1.65Second GenerationBrodifacoum 0.2 Bromadiolone 1.1Difenacoum 1.8Difethialone 0.6 Flocoumafen 0.2
Chronic vs. Acute Toxicity
Jackson, W.B., Ashton, A.D. 1992. A review of available anticoagulants and their use in the United States. Proc Vertebr Pest Conf 15:156-60.
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Modes of Action
Affects the Nervous System
Affects Energy Production
Insecticides
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Overview of the nervous system
Axon Axon
OrSynapseNerve cell
MuscleNeuromuscular junction
AcetylcholineAcetylcholinesterase
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Organochlorine Insecticides
Axon
Nerve cell
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Nicotinoid Insecticides
AxonAxon
Synapse
Acetylcholine
Imidacloprid
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Phenylpyrazole Insecticides
Nerve cell
Axon
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Organophosphorus and Carbamate Insecticides (Anticholinesterases)
Acetylcholine
Cholinesterase
Axon Axon
Synapse
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Direct vs. Latent Anticholinesterases
Direct inhibitors - some OPs and all CBsLatent inhibitors - some Ops
Delayed Neurotoxicity
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Biomarkers
Specific Non-specific
Exposure Effects Exposure
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Species
SpeciesMean Brain
ChE Activity American black duck 8
Least sandpiper 22
Common grackle 20Barn swallow 12
Mourning dove 16
Units: μmoles of acetylthiocholine iodide hydrolyzed per min per g of tissue, wet weight at 250C.
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Life History
Units: μmoles of acetylthiocholine iodide hydrolyzed per min per g of tissue, wet weight at 250C.
Species YoungAge
(days)ChE activity as
% of adult
Mallard Precocial 1 80
Starling Altricial 4 25
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Brain Regions(white-throated sparrow) Mean ChE activity
Basal ganglia 25
Hippocampus 15
Hypothalamus 14
Brain Regions
Units: μmoles of acetylthiocholine iodide hydrolyzed per min per g of tissue, wet weight at 250C.
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Normal neurotransmitter Activity
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Normal ChE Activity
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Pesticide Exposure
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ChE Inhibition
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Recovery: Spontaneous Reactivation with CBs
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Recovery: New ChE synthesis with OPs
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Cholinesterase Activity Measurement
ExposedControl
25% activity of controlor
75% activity inhibited compared to control
vs.
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Traditional ChE AnalysisAdvantage
• Direct biomarker for OP and CB exposure and effects.
Limitations
• Does not differentiate between OPs and CBs. Need residue analysis $$$$$.• Only useful when appropriate control values are available.
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Cholinesterase Reactivation
Exposed Reactivated
66% reactivated
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CB ReactivationIncubate to get spontaneous reactivation
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OP Reactivation: 2-PAM
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Aging of OPs Dimethyl OPs < 24 hrsEthyl-OPs within a few days
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Cholinesterase Reactivation
Unknown sample
2PAM 38oC
Cholinesterase analysis
ChE activity
If ChE activity then OP. If no change in ChE activity then not OP or Aging.
If ChE activity then CB. If no change in ChE
activity then not CB.
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Cholinesterase Measurements
Plasma vs. BrainAdvantages and Disadvantages
Why is brain cholinesterase a specific biomarker?
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Photo by Tonie Rocke
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Halogenated PyrrolesMetabolic Pathway
Glycolysis
Krebs Cycle
Oxidative Phosphorylation
Glucose
Energy
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Implications• Recovery of affected birds can improve the
estimate of the magnitude of a mortality incident.
• The magnitude of a kill is a critical element of the prosecution.
• The magnitude of a kill can affect the sentencing guidelines and help understand the hazards from a pesticide use.