BEST 2000 Characteristics

BEST 2000 (Biokit Elisa System) is a bench top analyser with capacity for 4 micro plates. Multiple assays can be performed per plate. BEST 2000 offers the user an easy to use sample-in /result-out processor.

The instrument automates all steps of the ELISA process:•Positive sample and plate barcode identification

•Sample, control and calibrator dilution and dispensing (disposable tip)•Reagent dispensing•Incubation (with or without shaking)•Washing•Reading•Data reduction

Specifications BEST 2000 Dimensions and WeightDepth <91 cm (35.8 in)Width <106 cm (41.8)Height <80 cm (31.5 in)Weight <110 Kg (243 lbs)Footprint 106 cm x 91 cm (41.8 in x 35.8 in)

CapacitiesSamples 96Reagents 24Controls and/or Standards 33Sample Pipetting Tips 432Reagent Pipetting Tips 41Wash Buffer Bottles 4 bottles, 2 liters eachWaste Container 8 litersOperationAmbient Drawer ModuleIncubation Temperatures Ambient plus 5 °CIncubator Module

Incubation Temperatures Ambient plus 7 °C to 50 °CAssaysBlanking AirIndividual, paired or average wellsWhole plate or last plate, Row or columnEach well on the plateWavelength Modes Single (10 sec) or dual (20 sec)Standard Curves Linear, quadratic, cubic, quartic, spline, polygon, sigmoid or AkimaAdditional Data Analysis Threshold, ratio, QC equationsFlexible Template Up to eight different well types.

Power RequirementsVoltage Power FrequencyMain Unit 100 - 240 V 800 VA 50/60 HzLine Voltage Variation ± 10%Line Frequency Variation ± 3 HzEnvironmentOperating Range 15° C to 30° C15% to 85% relative humidity (non-condensing)Altitude 2000 Meters

Chemwell Fully Automated Chemistry AnalyzerStat Fax 303 plus EIA Strip Reader

Stat Fax 2600 EIA Plate Washer

Stat Fax 2100 EIA Plate Reader

Stat Fax 2200 Plate Shaker/Incubator Stat Fax 3100 Manual Microwell Washer

ChemWell® is a fully automated analyzer capable of running both EIAs and general chemistries in standard micro wells.

Awareness Technology, Inc. is proud to announce the release of the updated Rev 6.0 Software. Currently it is available for biochemistries ONLY, but the EIA capable

version is nearing completion as well. (for ChemWells with microplate washers, please find out about Rev 5.11. This software allows you to run the EIA's). Some of the features of the new Rev 6.0 software are:

•Continous Loading - Instead of collecting a batch of specimens for a run, tests and samples can now be run on demand, even while other tests are running. •Calibrators and Controls can be run at any time and independently of tests, allowing you to update stored curves and validate tests as required. If tests associated with the newly loaded calibrator were already running, the sample concentrations for that test will be automatically adjusted for the new curve.

•Results for individual samples can be accepted or re-run for verification, simply by clicking a button. You will no longer have to setup a new job or reposition sample cups when re-testing. •Sample and Reagent positions can be moved around using the Windows® "drag and drop" feature. •Assay programming has been greatly simplified. •Reports format easier to modify. •Intuitive new interface.

ChemWell® is a completely open system that is easy to program. Set up assays, routine jobs, quality control, panels, and even index calculations to suit your laboratory. With a completely open system you can program additional wash volume, increase the number of washes, or even direct the probe to pick up and dispense a probe cleaning solution after each specimen. And it is entirely password protected.Precise pipetting of low volume specimens, elimination of carryover, and careful control of temperature are all essential for reliable

results. ChemWell® can make pre-dilutions, however, this is often not necessary since a high level of precision is achieved even with a 2uL sample. ChemWell® can process EIA at ambient temperature or 37C. An incubator is provided for biochemistry wells to provide precise kinetic reactions. The probe is also temperature controlled to deliver 37C reagents.RCA - Reagent Cooling Accessory <RCA.htm> now available for ChemWell®. Protects reagents from ambient heat. The removable racks can be refrigerated, pre-loaded, and ready for the next use.

ChemWell® is designed to maximize dependability, versatility, and economy.ChemWell® uses standard, off-the-shelf microwells for all reactions. Performing biochemistry assays in microwells offers many advantages such as increased throughput and decreased cost per test.

Probe washes inside and out

Washes 8 wells at once

Uncoated microwells make low volume sample cups for biochemistry. They can also be washed for re-use. ChemWell® reads vertically using 4 optical channels and 8 wavelengths.Handles up to 27 reagents, 96 samples.

Here are more features that you can expect with ChemWell®:

· 200 tests per hour · No carry-over · Liquid sensing probe tip · 8-well wash head · Level-sensing wash, rinse, and waste bottles · LIS, STAT · QC tracking · Self-monitoring

· Reflex testing · Reaction Volume <250uL · No custom disposables · IAD filters · Creates automatic service reports · Edit standard curves · self-monitoring mechanics and optics

All of this and more in one instrument, the ChemWell®.

Awareness Technology, Inc. of Palm City, Florida has been selected to receive the 2005 Governor's New Product Award for their innovative ChemWell 2910 automatic laboratory analyzer.

This award, co-sponsored by the Florida Engineering Society, in cooperation with the Governor's Office, recognizes companies with the foresight to bring new products to the marketplace. The purpose of the Governor's New Product Award is to promote the full

ChemWell Analyzer Wins State of Florida Recognition

spectrum of benefits that come from the research and engineering of new products and improve quality of life. This is the 19th year of the program and the first time the award will go to the clinical diagnostic industry.

Awareness Technology, a 23 year old Florida designer and manufacturer of lab instruments, competed with its new product, ChemWell 2910, which combines two fully automatic instruments into one economical package: a continuous loading biochemistry analyzer and a user-programmable enzyme immunoassay (EIA) system. This bench-top instrument performs all test processes including

dispensing and diluting reagents, incubating, washing, optical reading, calculating, and reporting results.

Mary Freeman, president of Awareness Technology, will accept the award on April 6 in the Florida capital where it will be presented by Mark Mongeau, PE, President of the Florida Engineering Society. State winners are eligible to compete for the national New Product award.

•Automatic strip transportation. •3-strip loading capacity or break-apart, single, and double strips. The carriers come in two styles, 3x12 for break-apart microwell strips, and an optional 3x8 for non-break-apart microwell strips. •Bichromatic optics. Standard model includes four filters, six filters are available for a nominal charge.

•Easy to use with step-by-step prompting and on-board graphics printer. •log-logit mode and other programming enhancements. •On-board software calculates single & multi-point calibration curves, •regressions, & a variety of cutoff options. •Large non-volatile memory stores more than 50 user-entered tests. •NRTL and CE certified.

•Fully automatic. •Bichromatic optics. Standard model includes four filters, six filters are available for a nominal charge. •On board data reduction calculations include regressions, curve fitting and cutoff absorbance calculations with step-by-step user prompting. •Large, non-volatile memory stores at least 50 user tests.

•Includes parallel and serial outputs. •NRTL and CE certified.

•Fully automatic. •Washes flat, round, and V-bottom plates and strips. •No user adjustments required. Automatic calibration, alignment, and last row detection. •Large, non-volatile memory stores at least 50 user wash protocols. •Precision operation with constant monitoring of vacuum and pressure. Pump cycles only when necessary

•for quiet operation. •Programmable automatic rinse cycle. •Ready to run: bottles, aerosol shield, 8-way manifold included. •12 and 16 position wash heads available as accessories. •NRTL and CE certified.

•Holds two 96 well microplates. •Digitally controlled 8-speed shaker with orbital mixing from 575 to 1500 rpm. •Temperature adjustable from ambient to 40°C with resolution of 0.1°C. •Digital timer controls shaking or can be operated independently as a timer. •Compact and easy to operate.

•Outer tinted cover provides further insulation and protection from light. •NRTL and CE certified

•No more squirt bottle washing. Reduce exposure to the potential dangers of splashing biohazards with Stat Wash®, an economical instrument designed to wash immunoassays in microwell strips and plates with professional precision. Washing 8 wells simultaneously is faster too.

•Aspirating and dispensing are controlled by push buttons. Easy to use and maintain, Stat Wash® includes an 8-probe manifold, a 1L wash bottle, and a 1L safe plastic bottle with a full sensor. Stat Wash™ is CE certified.

•If your lab doesn't need an expensive automated instrument, simply switch to Stat Wash® for clean, fast, and precise results.

Biokit, Spain

· Bioelisa HCV· Bioelisa HBsAg· Bioelisa HIV· Bioelisa Syphlis

Standard Diagnostic, Korea

· HCV· HBsAg· HIV· Malaria· Syphilis

DiaSys, Germany· ATP Hexokinase

Solid phase: Guinea pig polyclonal antibodies anti-HBs subtype ad+ay

Conjugate: Goat polyclonal antibodies anti HBs subtype ad+ay

Polyclonal antibodies assure the detection of possible virus mutations .

Cod: 3000-1100 96 TestsCod. 3000-1180 480 Tests

TMB

1 hour 37°C 30 min 37°C 30 min RT

Qualitative assay: COV:Negative+0,040Sensitivity:

- 0,034 PEI/ml ad antigen- 0,024 PEI/ml ay antigen- 0,054 UI/WHO standard- 0.2 ng/ml

Specificity: 99,9%

Solid phase: Recombinant antigens from the Core, NS3 NS4 and NS5 regions

Conjugate: Anti-human IgG labelled with peroxidase

Sample dilution 1/20 in plate (IPD)Qualitative assay:- COV: Negative + 300

Sensitivity: 99,9%

Specificity: 99.87 %

TMB

1 hour 37°C 30 min 37°C 30 min RTCod: 3000-1117 96 TestsCod. 3000-1119 480 Tests

• Solid phase: Recombinant antigens: HIV-1: p24, gp41, gp120 HIV-2: gp36, gp125 Breakable wells

• Conjugate: Anti-human IgG and IgM labelled with peroxidase

• Sample dilution in plate 1/20 (IPD)• Subtype O Detection

• Qualitative assay: COV: Positive control x 0.32

• Sensitivity: 100%• Specificity: >99.5%

OPD

Cod: 3000-1143 96 TestsCod. 3000-1144 480 Tests

Speed Control: Digital – Variable (100 rpm inc.)Max. Speed: 6500 rpmMax g-force 4000xgCapacity: 6x10mlTimer: 1-30 min or continuousRotor angle: 30 degreeDimentions (WxDxH) 8.25x9.5x7 in (21x24x18cm)Weight: 10lbs / 4.5 kgElectrical: 230V, 50-60 Hz

Spectrafuge 6C