Auto•Blend Plus TechnologyAuto•Blend Plus Technology for Ion Exchange, Size Exclusion, and...

Auto•Blend PlusTechnology

for Ion Exchange, Size Exclusion, andReversed-phase Chromatography

716004587 / Revision A

Copyright © Waters Corporation 2015All rights reserved

*716004587* *Rev.A*

General information

Copyright notice© 2015 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICAAND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOFMAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OFTHE PUBLISHER.

Trademarks

ACQUITY® is a registered trademark of Waters Corporation.

Auto•Blend PlusTM is a trademark of Waters Corporation.

Empower® is a registered trademark of Waters Corporation.

THE SCIENCE OF WHAT'S POSSIBLE® is a registered trademark of WatersCorporation.

Customer commentsWaters’ Technical Communications organization invites you to report any errors thatyou encounter in this document or to suggest ideas for otherwise improving it. Helpus better understand what you expect from our documentation so that we cancontinuously improve its accuracy and usability.

We seriously consider every customer comment we receive. You can reach us [email protected].

Contacting WatersContact Waters with enhancement requests or technical questions regarding the use,transportation, removal, or disposal of any Waters product. You can reach us via theInternet, telephone, or conventional mail.

June 11, 2015, 716004587 Rev. APage ii

MAILTO:[email protected]

Waters contact information

Contactingmedium

Information

Internet The Waters Web site includes contact information for Waterslocations worldwide.Visit www.waters.com

Telephone and fax From the USA or Canada, phone 800-252-4752, or fax508-872-1990.For other locations worldwide, phone and fax numbers appearin the Waters Web site.

Conventional mail Waters CorporationGlobal Support Services34 Maple StreetMilford, MA 01757USA

June 11, 2015, 716004587 Rev. APage iii

HTTP://WWW.WATERS.COM

Table of contents

General information ......................................................................ii

Copyright notice.............................................................................................ii

Trademarks ...................................................................................................ii

Customer comments.......................................................................................ii

Contacting Waters ..........................................................................................ii

Auto•Blend Plus Technology for Ion Exchange, Size Exclusion,and Reversed-phase Chromatography...........................................5

Instrument Method Editor............................................................................... 6Gradient graph........................................................................................7

Solvent catalog ............................................................................................. 8Solvent catalog icons ...............................................................................8Exporting the solvent catalog ....................................................................9Importing the solvent catalog....................................................................9

Auto•Blend Plus methods ..............................................................................10Using a defined Auto•Blend Plus buffer system.......................................... 10Creating a new buffer system.................................................................. 13Creating a new solvent........................................................................... 15Modifying a solvent................................................................................ 16Example of a wide pH-range buffer system ............................................... 16

pH calibration for a buffer system...................................................................18Calibrating pH using a pKa value ............................................................. 18Calibrating pH using an empirical data table.............................................. 19

Safety advisories..........................................................................................20Warning symbols................................................................................... 20Notices ................................................................................................ 23Bottles Prohibited symbol ....................................................................... 23Required protection ............................................................................... 23Warnings that apply to all Waters instruments and devices ......................... 24Warnings that address the replacing of fuses............................................. 28Electrical symbols.................................................................................. 30Handling symbols .................................................................................. 30

June 11, 2015, 716004587 Rev. APage iv

Auto•Blend Plus Technology forIon Exchange, Size Exclusion, andReversed-phase Chromatography

Auto•Blend Plus technology uses pure solvents and concentrated stocks to blendmobile phase compositions at a specific pH. At the same time, it controls theconcentration of salt or organic solvent to optimize separations.

Use the Auto•Blend Plus feature to create and store buffer systems in a solventcatalog that allows users of an ACQUITY quaternary solvent manager to share.

To prepare and adjust chromatographic mobile phases on demand, you addconcentrated acid, base, salt or organic solvent, and water to the solvent reservoirsand set up the desired gradient by pH instead of percent solvent composition. Bydoing so you can, for example, optimize protein separations, which are especiallysensitive to a buffer's pH and salt concentration. You can also optimize reversed-phase separations that are sensitive to pH and organic-solvent composition.

For safety advisory information, consult the applicable, system-specific, quaternarysolvent manager overview and maintenance guide.

Warning: Observe Good Laboratory Practice (GLP) at all times, particularlywhen working with hazardous materials. Consult the Material Safety DataSheets regarding the solvents you use. Additionally, consult the safetyrepresentative for your organization regarding its protocols for handling suchmaterials.

June 11, 2015, 716004587 Rev. A

Instrument Method Editor

Note: The High and Low pressure limits are located on the Misc tab.

Convert the Instrument Method Editor from compositional gradient mode toAuto•Blend Plus mode

Open the Solvent Catalog

Columns Calculator

Display both a compositional gradient table and the Auto•Blend Plus gradienttable

June 11, 2015, 716004587 Rev. A

Graph of the gradient

Full gradient table view

The Recommended pH range is defined during the creation of a buffer systemin the Buffer System Details window

The Concentration to deliver is auto-populated from the BufferConcentration to Deliver in the Buffer System Details window

Gradient graph

June 11, 2015, 716004587 Rev. A

Click to add or remove the associated solvent from the gradient graph

Click to add or remove the organic or salt concentration-curve from the gradientgraph

Note: Only curves 6 and 11 display correctly in the gradient graph.

Click to add or remove the pH-curve from the gradient graph

Note: Only curves 6 and 11 display correctly in the gradient graph.

Solvent catalogNote: The solvent catalog contains defined solvents as well as defined buffersystems.

Anytime that you create and save a new solvent or buffer system, it is saved to thesolvent catalog. The Auto•Blend Plus feature makes available a laboratory's solventcatalog to any other lab in the world that uses a Waters ACQUITY quaternary solventmanager.

Click , to access the Solvent Catalog.

Solvent catalog icons

Icon Description

Add a new solvent or buffer system

June 11, 2015, 716004587 Rev. A

Icon Description

Remove the selected solvent or buffer system

Add a new solvent or buffer system as a copy of one selected

Rename the selected solvent or buffer system

View or edit the details of the selected solvent or buffer system

Filter the list of displayed solvents or buffer systems

Reverse the selection state of all solvents or buffer systems

Import or export solvent or buffer system definitions

Print solvent or buffer system details

Select Details, to show additional information and comments abouteach solvent or buffer system

Note: If a default solvent or buffer system is selected, you cannot select or .

Exporting the solvent catalog1. Select solvents and buffer systems for exporting.

2. Click .

3. Specify the destination where the exported items will be saved.

4. Specify a file name, and then click Save.

Result: The solvent or buffer system is saved as a .xml file.

Importing the solvent catalog

1. Click .

2. Select the .xml file that corresponds to the solvent or buffer system that youwant to import to the solvent catalog.

3. Click Import.

Result: The selected solvents and buffer systems can now be viewed or usedfrom your solvent catalog.

June 11, 2015, 716004587 Rev. A

Auto•Blend Plus methodsBy automating the formulation of mobile phases from reservoirs of pure solvents orconcentrated stock solutions, an Auto•Blend Plus method dynamically creates pH,salt, and solvent gradient conditions. You can modify a defined buffer system, orcreate a new one.

Note: The Auto•Blend Plus feature offers several default buffer systems and solventdefinitions.

Using a defined Auto•Blend Plus buffer system1. From the Empower, Run Samples, click Edit > Instrument Method.

Result: New methods for the solvent manager always open in composition-gradient mode.

2. From the quaternary solvent manager Instrument Method Editor, click Auto•Blend Plus.

3. Click Yes, to confirm the conversion of the method to Auto•Blend Plus mode.

Tip: If you decide not to create an Auto•Blend Plus method, close theinstrument method editor without saving it, and then reopen it from thechromatography software.

4. From the list of previously defined buffer systems, select a salt or organicbuffer system.

Result:

• If you open a salt buffer system, the gradient table contains columns fortime, flow, pH, pH curve, salt concentration, and salt curve.

• If you open an organic buffer system, the gradient table contains columnsfor time, flow, pH, pH curve, percent organic, and organic curve.

Tip: To easily locate a defined buffer system that suits your chromatography,select Show Salts or Show Organics.

5. On the Solvents tab, specify which solvent is in each of the four reservoirs, A,B, C, and D.

Tips:

• The Solvents tab is the only place where you can define the reagent ineach solvent reservoir. When the method is running, the Auto•Blend Plussoftware calculates the amount of each solvent required to obtain thedesired gradient according to the definitions on this tab.

• Establishing a convention such as A, for acid, B, for base, C, for salt ororganic solvent, and D, for aqueous solvent, decreases the possibility ofputting the wrong solvent in one or more reservoirs.

June 11, 2015, 716004587 Rev. A

Note: If the solvent manager is fitted with an optional solvent-selection valve,you can select D1 through D6 in the field next to solvent D. The valve is alwaysassociated with the D reservoir. For systems configured with a solvent-selection valve, assigning different physical reservoirs to the specified solventtype becomes important.

6. Ensure that four solvent reservoirs are installed in the solvent tray.

7. Fill the solvent reservoirs with the solutions defined in the specified buffersystem, and ensure that the reservoirs are filled with the correct solvent foreach line as defined on the Solvents tab.

June 11, 2015, 716004587 Rev. A

8. On the General, tab specify each of the following parameters in the gradienttable:

Parameter Description

Time Range: 0.00 through 599.99 minutes

Flow Range: 0.000 through 5.000 mL/min

Note: The maximum flow rate depends on your systemconfiguration and specified parameter settings.

pH Range (typing entry limit): 2.0 through 12.0

Note: The allowed limits for pH vary according to the typeand strength of buffers used.

Saltconcentrationor organicpercentage

Range: 0 to 10,000 mM or 0 to 100%

Note: The allowed limits for salt concentration or organicpercentage vary according to the type and strength of buffersused.

Curve Specifies the rate at which the solvent changes to the newproportions, flow rates, or both. You can select one of the 11predefined curves for the pH and the salt concentration ororganic percentage.

• 1: Pre-step

• 2–5: Convex

• 6: Linear

• 7–10: Concave

• 11: Post-step

Notes:

June 11, 2015, 716004587 Rev. A

• You can specify separate and independent curves for pH and saltconcentration. The software can adjust both pH and salt concentrationsimultaneously to optimize the separation for a particular protein mixture.

• You can specify separate and independent curves for pH and organicconcentration. The software can adjust both pH and organic concentrationsimultaneously to optimize the separation.

9. Select File > Save As, enter the method name, and then click Save.

Note: The software reviews the calculations in the gradient table when youclick Save. An error message appears if the software encounters an invalidentry. For example, if the Recommended pH range for a specified buffersystem is 2.95 to 3.79, and in the gradient table, you specified a value thatfalls outside that range, an error message prompts you to specify a pH withinthe range.

Creating a new buffer system1. From the Empower, Run Samples window, click Edit > Instrument Method.

Result: New methods for the solvent manager always open in composition-gradient mode.

2. From the quaternary solvent manager Instrument Method Editor, click Auto•Blend Plus.

3. Click Yes, to confirm the conversion of the method to Auto•Blend Plus mode.

Tip: If you decide not to create an Auto•Blend Plus method, close theinstrument method editor without saving it, and then reopen it from thechromatography software.

4. In the Auto•Blend Plus method editor, click , to open the Solvent Catalog.

5. In the Filter by field, select Buffer System.

6. Click , to open the Solvent Details editor window, click OK.

7. Type a buffer system name.

8. Select Buffer/Salt or Buffer/Organic.

Note: Once a buffer system has been classified as either Buffer/Salt or Buffer/Organic it cannot be changed.

9. Specify the value in the Buffer Concentration to Deliver field, assigning avalue between 1 and 10,000 mM.

Requirement: The Buffer Concentration to Deliver value must be equal tothe sum of the acid and base concentrations, multiplied by the correspondingpercentages that appear in the empirical table. For example, the concentration

June 11, 2015, 716004587 Rev. A

of acid and base is 125 mM. To deliver 25 mM of buffer to the column, thepercentage of acid and base in each row of the empirical table must total 20%.If instead you want to deliver 12.5 mM of buffer to the column, the percentageof acid and base must total 10%. For more information, see Bufferconcentration to deliver.

10. For each solvent type, from the list, select a solvent, or select [New...], tocreate a new solvent.

11. Choose the calibration of the buffer system by entering a pKa value or using anempirical data table.

12. In the Comment area, record the separations for which the buffer system isused.

13. Click OK.

Result: The buffer system is automatically saved and added to the SolventLibrary.

Buffer concentration to deliver

You must specify the concentration of buffer to deliver to the column. In doing so,you must specify these items:

• Identity and concentration of the buffer stocks

• Total percentage of flow allowed for the buffers and for the strong solvent or salt

The Buffer Concentration to Deliver represents the total amount, in moles, of acidand base used to control the pH of the mobile phase.

The equation for determining buffer concentration to deliver is:

Buffer Concentration to Deliver = (% acid)(concentration of acid stock) + (% base)(concentration of base stock)

Specify the values according to your knowledge of the analytes' properties and therequired chromatographic mechanisms.

In ion-exchange and reversed-phase chromatography, a buffer concentration ofapproximately 25 mM for a pH near the pKa of the buffering ion usually controlsretention and selectivity. Depending on your chemistry and system configuration:

• Using a lower Buffer Concentration to Deliver can improve detector sensitivity.

• You can use a higher Buffer Concentration to Deliver for a pH that is furtherfrom the point of maximum buffering capacity at the pKa.

• More buffering can be necessary for injected samples that are at a significantlydifferent pH.

• Some columns require a high Buffer Concentration to Deliver, to eliminatesecondary interactions.

June 11, 2015, 716004587 Rev. A

Note: It is seldom necessary to increase the concentration of strong solventbeyond 80% in reversed phase systems or above 0.5 M in ion-exchange systems.(For those maximal ranges, take care to avoid buffer precipitation.) A reasonablestarting point, therefore, is to reserve 80% of the flow composition for salt ororganic solvent and 20% of the flow for buffer. In such a case, the concentrationof the stock buffers would then be 5-fold higher than the intended finalconcentration. The sum of the acid and the base would be a constant 20%.

Calculating the concentration of buffer to deliver

The following example explains how to calculate the concentration of buffer todeliver:

Assume that the target mobile phase (buffer/organic solvent) is 25 mM ammoniumformate over a pH range 2.95 to 3.79:

• Maximum organic concentration = 80% (acetonitrile)

• Buffer proportions = (% acid) + (% base) = 20%

• 125 mM = concentration of acid stock

• 125 mM = concentration of base stock

Buffer Concentration to Deliver = (% acid)(concentration of acid stock) + (% base)(concentration of base stock) = (0.1)(125 mM) + (0.1)(125 mM) = 25 mM

Assuming a pH of 3.5, 13.7% (125 mM acid) + 6.3% (125 mM base) = 25 mMammonium formate.

Thus, the value specified in the Buffer Concentration to Deliver field would be 25mM.

Creating a new solvent


2. In the Filter by field, select the type of solvent you want to create.

Note: If Buffer System is displayed in the Filter by field, the correct windowwill fail to open.

3. Click , to open the solvent type Details (Modified) window, click OK.

4. In the Name field, type a new name.

5. Specify a concentration value (allowed: 0 to 10,000 mM).

Note: If you are creating an organic solvent, the software sets the organicconcentration to 100%.

June 11, 2015, 716004587 Rev. A

6. If desired, in the Comment area, enter the recipe used to prepare the solvent.

7. Click OK.

Result: The window closes, and the new solvent is saved in the solventcatalog.

Modifying a solvent

Important: To properly save a solvent, click . If you modify a solvent fromwithin the Buffer System Details window it will fail to save correctly.

To modify a solvent:


2. In the Filter by field, select the solvent that you want to modify.

Note: If Buffer System is displayed in the Filter by field, the correct windowwill fail to open.

3. Click , to add a copy of the selected solvent.

Result: The solvent type Details (Modified) window opens.

4. In the Name field, type a new name.

5. Modify the necessary parameters.

6. Click OK.

Result: The window closes, and the modified solvent is saved in the solventlist.

Example of a wide pH-range buffer systemSimple buffer systems operate on the basis of a single, buffering-ion that functionsover a limited pH range. Such systems, however, are not always useful. A buffersystem that functions over a wider range of pH can be necessary in someapplications. Wide pH-range buffers can be used in complex systems for ionexchange, for screening a wide variety of samples, for testing a wide pH range, andfor creating pH gradients.

When creating a wide pH-range buffer system, you combine several buffers into onebuffer system, selecting individual buffering ions with pKs spaced throughout thedesired pH range. Thus you must perform these tasks:

• Identify free bases and acids that function over a desired pH range.

• Prepare an empirical table.

June 11, 2015, 716004587 Rev. A

An example of a complex buffer system appears in the software: "pH 5.3–9.7 MultiBuffer A - Proteins 20mM". That system comprises three pairs of acid and base saltsin the concentrations shown in the following table.

Table 1–1: Acid mixture

Compound Concentration (mM)

Tris-Cl 48.00

Imidazole-Cl 30.00

Piperazine-Cl 58.00

Table 1–2: Base mixture

Compound Concentration (mM)

Tris 48.00

Imidazole 30.00

Piperazine 58.00

To create the empirical table, the pH value for varying paired proportions of the acidand base are measured and then plotted. The table and graph below show thevolumes of four solvents, the acid mixture (A), the base mixture (B), acetonitrile (C),and water (D), that determine the pH values for the empirical table. After the valuesare recorded, you can refer to the table when setting up the buffer system in theinstrument method.

The software requires a value in the Buffer Concentration to Deliver field, forcomputational reasons. In multiple buffer systems, a concentration of 20 mM for thisvalue is arbitrarily adopted as is a concentration of 100 mM for the stockconcentrations of the acid and base mixtures. By assigning these values, the sum ofthe acid and base remains a constant 20%.

June 11, 2015, 716004587 Rev. A

Figure 1–1: Multi-Buffer Empirical Table

pH calibration for a buffer systemCalculating the proportions of acid and base depends on the pH calibration. Thesoftware calculates the percentage flow from each reservoir on the basis ofinformation defined in the buffer system. You can specify the calibration in two ways,by pKa value or empirically. The empirical table provides the most accuratelydelivered gradient because it can automatically compensate for changes resultingfrom ionic strength or organic concentration.

Calibrating pH using a pKa value1. In the Buffer System Details (Modified) window, select pKa.

2. Enter the published (literature) pKa value or use the measured pH of the bufferpair in use as the pKa value.

Note: To determine the measured pKa of the buffer pair, prepare a solution ofequimolar amounts of the acid and base. Add water and salt or organic solvent,to match the salt or organic concentration at the midpoint of the gradient. Thenmeasure the solution's pH.

3. Click OK.

June 11, 2015, 716004587 Rev. A

Calibrating pH using an empirical data table1. In the Buffer System Details (Modifed) window, select Empirical Data.

Result: A dialog box appears asking whether you want to fill the empiricaltable using the standard template.

2. Click Yes to populate the empirical data table with the standard template.

Note: The standard template is a nine-point calibration matrix correspondingto low, intermediate, and high salt or organic concentrations at low,intermediate, and high pH values (relative to the pKa). The nine-pointcalibration is sufficiently accurate for most separations.

Figure 1–2: Example

Alternative: If the standard nine-point calibration table is not sufficient forthe desired separation, click No and enter the desired percent concentrationsfor the required calibration.

3. Referring to the percentages displayed in the table, prepare a series of manualblends of the four solvents.

4. Measure the pH of the first blend with a pH meter, and enter the observedvalue in the pH column.

5. Repeat step 4 for the remaining blends.

6. Click OK.

Note: If the message The buffer concentration to deliver and theconcentration of the stocks are incompatible with the percentagesof the acid and base in the empirical table. appears, ensure thatthese conditions are satisfied:

June 11, 2015, 716004587 Rev. A

• The values in the empirical table are correct. The percent composition ofeach solvent in each row has to total 100%.

• The value in the Buffer Concentration to Deliver field is equal to the sum ofthe concentrations of acid and base multiplied by the correspondingpercentages used in the empirical table. For example, the concentration ofacid and base is 125 mM. To deliver 25 mM to the column, the percentageof acid and base in each row of the empirical table must total 20%. If,instead, you want to deliver 12.5 mM of buffer to the column, thepercentage of acid and base must total 10%. See Buffer concentration todeliver, for more information.

Safety advisoriesWaters instruments and devices display hazard symbols that alert you to the hiddendangers associated with a product’s operation and maintenance. The symbols alsoappear in product manuals where they accompany statements describing the hazardsand advising how to avoid them. This appendix presents the safety symbols andstatements that apply to all of Waters’ product offerings.

Warning symbolsWarning symbols alert you to the risk of death, injury, or seriously adversephysiological reactions associated with the misuse of an instrument of device. Heedall warnings when you install, repair, or operate any Waters instrument or device.Waters accepts no liability in cases of injury or property damage resulting from thefailure of individuals to comply with any safety precaution when installing, repairing,or operating any of its instruments or devices.

The following symbols warn of risks that can arise when you operate or maintain aWaters instrument or device or component of an instrument or device. When one ofthese symbols appear in a manual’s narrative sections or procedures, anaccompanying statement identifies the applicable risk and explains how to avoid it.

Warning: (General risk of danger. When this symbol appears on aninstrument, consult the instrument’s user documentation for importantsafety-related information before you use the instrument.)

Warning: (Risk of burn injury from contacting hot surfaces.)

Warning: (Risk of electric shock.)

Warning: (Risk of fire.)

June 11, 2015, 716004587 Rev. A

Warning: (Risk of sharp-point puncture injury.)

Warning: (Risk of hand crush injury.)

Warning: (Risk of injury caused by moving machinery.)

Warning: (Risk of exposure to ultraviolet radiation.)

Warning: (Risk of contacting corrosive substances.)

Warning: (Risk of exposure to a toxic substance.)

Warning: (Risk of personal exposure to laser radiation.)

Warning: (Risk of exposure to biological agents that can pose a serioushealth threat.)

Warning: (Risk of tipping.)

Warning: (Risk of explosion.)

Specific warnings

Burst warning

This warning applies to Waters instruments and devices fitted with nonmetallictubing.

June 11, 2015, 716004587 Rev. A

Warning: To avoid injury from bursting, nonmetallic tubing, heed theseprecautions when working in the vicinity of such tubing when it ispressurized:

• Wear eye protection.

• Extinguish all nearby flames.

• Do not use tubing that is, or has been, stressed or kinked.

• Do not expose nonmetallic tubing to compounds with which it ischemically incompatible: tetrahydrofuran, nitric acid, and sulfuric acid, forexample.

• Be aware that some compounds, like methylene chloride and dimethylsulfoxide, can cause nonmetallic tubing to swell, significantly reducing thepressure at which the tubing can rupture.

Biohazard warning

The following warning applies to Waters instruments and devices that can processmaterial containing biohazards, which are substances that contain biological agentscapable of producing harmful effects in humans.

Warning: To avoid infection with potentially infectious, human-sourcedproducts, inactivated microorganisms, and other biological materials,assume that all biological fluids that you handle are infectious.Specific precautions appear in the latest edition of the US National Institutesof Health (NIH) publication, Biosafety in Microbiological and BiomedicalLaboratories (BMBL).Observe Good Laboratory Practice (GLP) at all times, particularly whenworking with hazardous materials, and consult the biohazard safetyrepresentative for your organization regarding the proper use and handlingof infectious substances.

Biohazard and chemical hazard warning

This warning applies to Waters instruments and devices that can process biohazards,corrosive materials, or toxic materials.

June 11, 2015, 716004587 Rev. A

Warning: To avoid personal contamination with biohazards, toxic materials, orcorrosive materials, you must understand the hazards associated with their handling.Guidelines prescribing the proper use and handling of such materials appear in thelatest edition of the National Research Council's publication, Prudent Practices in theLaboratory: Handling and Management of Chemical Hazards.Observe Good Laboratory Practice (GLP) at all times, particularly when working withhazardous materials, and consult the safety representative for your organizationregarding its protocols for handling such materials.

NoticesNotice advisories appear where an instrument or device can be subject to use ormisuse that can damage it or compromise a non-clinical sample’s integrity (risks toclinical sample integrity are accompanied by warning symbols). The exclamationpoint symbol and its associated statement alert you to such risk.

Notice: To avoid damaging the instrument’s case, do not clean it withabrasives or solvents.

Bottles Prohibited symbolThe Bottles Prohibited symbol alerts you to the risk of equipment damage caused bysolvent spills.

Prohibited: To avoid equipment damage caused by spilled solvent, do notplace reservoir bottles directly atop an instrument or device or on its frontledge. Instead, place the bottles in the bottle tray, which serves assecondary containment in the event of spills.

Required protectionThe Use Eye Protection and Wear Protective Gloves symbols alert you to therequirement for personal protective equipment. Select appropriate protectiveequipment according to your organization’s standard operating procedures.

Requirement: Use eye protection when refilling or replacing solventbottles.

Requirement: Wear clean, chemical-resistant, powder-free gloves whenhandling samples.

June 11, 2015, 716004587 Rev. A

Warnings that apply to all Waters instruments and devicesWhen operating this device, follow standard quality-control procedures and theequipment guidelines in this section.

Warning: Changes or modifications to this unit not expressly approved bythe party responsible for compliance could void the user’s authority tooperate the equipment.

Avertissement : Toute modification sur cette unité n’ayant pas étéexpressément approuvée par l’autorité responsable de la conformité à laréglementation peut annuler le droit de l’utilisateur à exploiter l’équipement.

Warnung: Jedwede Änderungen oder Modifikationen an dem Gerät ohnedie ausdrückliche Genehmigung der für die ordnungsgemäßeFunktionstüchtigkeit verantwortlichen Personen kann zum Entzug derBedienungsbefugnis des Systems führen.

Avvertenza: qualsiasi modifica o alterazione apportata a questa unità enon espressamente autorizzata dai responsabili per la conformità fadecadere il diritto all'utilizzo dell'apparecchiatura da parte dell'utente.

Advertencia: cualquier cambio o modificación efectuado en esta unidadque no haya sido expresamente aprobado por la parte responsable delcumplimiento puede anular la autorización del usuario para utilizar el equipo.

警告：未經有關法規認證部門允許對本設備進行的改變或修改,可能會使使用者喪失操作該設備的權利。

警告：未经有关法规认证部门明确允许对本设备进行的改变或改装，可能会使使用者丧失操作该设备的合法性。

경고: 규정 준수를 책임지는 당사자의 명백한 승인 없이 이 장치를 개조 또는 변경할경우, 이 장치를 운용할 수 있는 사용자 권한의 효력을 상실할 수 있습니다.

警告: 規制機関から明確な承認を受けずに本装置の変更や改造を行うと、本装置のユーザーとしての承認が無効になる可能性があります。

June 11, 2015, 716004587 Rev. A

Warning: Use caution when working with any polymer tubing underpressure:

• Always wear eye protection when near pressurized polymer tubing.

• Extinguish all nearby flames.

• Do not use tubing that has been severely stressed or kinked.

• Do not use nonmetallic tubing with tetrahydrofuran (THF) or concentratednitric or sulfuric acids.

• Be aware that methylene chloride and dimethyl sulfoxide causenonmetallic tubing to swell, which greatly reduces the rupture pressure ofthe tubing.

Avertissement : Manipulez les tubes en polymère sous pression avecprecaution:

• Portez systématiquement des lunettes de protection lorsque vous voustrouvez à proximité de tubes en polymère pressurisés.

• Eteignez toute flamme se trouvant à proximité de l’instrument.

• Evitez d'utiliser des tubes sévèrement déformés ou endommagés.

• Evitez d'utiliser des tubes non métalliques avec du tétrahydrofurane(THF) ou de l'acide sulfurique ou nitrique concentré.

• Sachez que le chlorure de méthylène et le diméthylesulfoxyde entraînentle gonflement des tuyaux non métalliques, ce qui réduit considérablementleur pression de rupture.

Warnung: Bei der Arbeit mit Polymerschläuchen unter Druck ist besondereVorsicht angebracht:

• In der Nähe von unter Druck stehenden Polymerschläuchen stetsSchutzbrille tragen.

• Alle offenen Flammen in der Nähe löschen.

• Keine Schläuche verwenden, die stark geknickt oder überbeanspruchtsind.

• Nichtmetallische Schläuche nicht für Tetrahydrofuran (THF) oderkonzentrierte Salpeter- oder Schwefelsäure verwenden.

• Durch Methylenchlorid und Dimethylsulfoxid können nichtmetallischeSchläuche quellen; dadurch wird der Berstdruck des Schlauches erheblichreduziert.

June 11, 2015, 716004587 Rev. A

Avvertenza: fare attenzione quando si utilizzano tubi in materialepolimerico sotto pressione:

• Indossare sempre occhiali da lavoro protettivi nei pressi di tubi dipolimero pressurizzati.

• Spegnere tutte le fiamme vive nell'ambiente circostante.

• Non utilizzare tubi eccessivamente logorati o piegati.

• Non utilizzare tubi non metallici con tetraidrofurano (THF) o acidosolforico o nitrico concentrati.

• Tenere presente che il cloruro di metilene e il dimetilsolfossido provocanorigonfiamenti nei tubi non metallici, riducendo notevolmente la pressionedi rottura dei tubi stessi.

Advertencia: se recomienda precaución cuando se trabaje con tubos depolímero sometidos a presión:

• El usuario deberá protegerse siempre los ojos cuando trabaje cerca detubos de polímero sometidos a presión.

• Si hubiera alguna llama las proximidades.

• No se debe trabajar con tubos que se hayan doblado o sometido a altaspresiones.

• Es necesario utilizar tubos de metal cuando se trabaje contetrahidrofurano (THF) o ácidos nítrico o sulfúrico concentrados.

• Hay que tener en cuenta que el cloruro de metileno y el sulfóxido dedimetilo dilatan los tubos no metálicos, lo que reduce la presión deruptura de los tubos.

警告：當在有壓力的情況下使用聚合物管線時，小心注意以下幾點。

• 當接近有壓力的聚合物管線時一定要戴防護眼鏡。

• 熄滅附近所有的火焰。

• 不要使用已經被壓癟或嚴重彎曲管線。

• 不要在非金屬管線中使用四氫呋喃或濃硝酸或濃硫酸。

• 要了解使用二氯甲烷及二甲基亞楓會導致非金屬管線膨脹，大大降低管線的耐壓能力。

June 11, 2015, 716004587 Rev. A

警告：当有压力的情况下使用管线时，小心注意以下几点：

• 当接近有压力的聚合物管线时一定要戴防护眼镜。

• 熄灭附近所有的火焰。

• 不要使用已经被压瘪或严重弯曲的管线。

• 不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸。

• 要了解使用二氯甲烷及二甲基亚枫会导致非金属管线膨胀，大大降低管线的耐压能力。

경고: 가압 폴리머 튜브로 작업할 경우에는 주의하십시오.

• 가압 폴리머 튜브 근처에서는 항상 보호 안경을 착용하십시오.

• 근처의 화기를 모두 끄십시오.

• 심하게 변형되거나 꼬인 튜브는 사용하지 마십시오.

• 비금속(Nonmetallic) 튜브를 테트라히드로푸란(Tetrahydrofuran: THF) 또는 농축 질산 또는 황산과 함께 사용하지 마십시오.

• 염화 메틸렌(Methylene chloride) 및 디메틸술폭시드(Dimethyl sulfoxide)는 비금속튜브를 부풀려 튜브의 파열 압력을 크게 감소시킬 수 있으므로 유의하십시오.

警告: 圧力のかかったポリマーチューブを扱うときは、注意してください。

• 加圧されたポリマーチューブの付近では、必ず保護メガネを着用してください。

• 近くにある火を消してください。

• 著しく変形した、または折れ曲がったチューブは使用しないでください。

• 非金属チューブには、テトラヒドロフラン(THF)や高濃度の硝酸または硫酸などを流さないでください。

• 塩化メチレンやジメチルスルホキシドは、非金属チューブの膨張を引き起こす場合があり、その場合、チューブは極めて低い圧力で破裂します。

This warning applies to Waters instruments fitted with nonmetallic tubing. Thiswarning applies to instruments operated with flammable solvents.

Warning: The user shall be made aware that if the equipment is used in amanner not specified by the manufacturer, the protection provided by theequipment may be impaired.

Avertissement : L’utilisateur doit être informé que si le matériel est utiliséd’une façon non spécifiée par le fabricant, la protection assurée par lematériel risque d’être défectueuses.

Warnung: Der Benutzer wird darauf aufmerksam gemacht, dass beiunsachgemäßer Verwendung des Gerätes die eingebautenSicherheitseinrichtungen unter Umständen nicht ordnungsgemäßfunktionieren.

June 11, 2015, 716004587 Rev. A

Avvertenza: si rende noto all'utente che l'eventuale utilizzodell'apparecchiatura secondo modalità non previste dal produttore puòcompromettere la protezione offerta dall'apparecchiatura.

Advertencia: el usuario deberá saber que si el equipo se utiliza de formadistinta a la especificada por el fabricante, las medidas de protección delequipo podrían ser insuficientes.

警告：使用者必須非常清楚如果設備不是按照製造廠商指定的方式使用，那麼該設備所提供的保護將被消弱。

警告：使用者必须非常清楚如果设备不是按照制造厂商指定的方式使用，那么该设备所提供的保护将被削弱。

경고: 제조업체가 명시하지 않은 방식으로 장비를 사용할 경우 장비가 제공하는 보호수단이 제대로 작동하지 않을 수 있다는 점을 사용자에게 반드시 인식시켜야 합니다.

警告: ユーザーは、製造元により指定されていない方法で機器を使用すると、機器が提供している保証が無効になる可能性があることに注意して下さい。

Warnings that address the replacing of fusesThe following warnings pertain to instruments and devices equipped with user-replaceable fuses. Information describing fuse types and ratings sometimes, but notalways, appears on the instrument or device.

Finding fuse types and ratings when that information appears on theinstrument or device:

Warning: To protect against fire, replace fuses with those of the type andrating printed on panels adjacent to instrument fuse covers.

Avertissement : pour éviter tout risque d'incendie, remplacez toujours lesfusibles par d'autres du type et de la puissance indiqués sur le panneau àproximité du couvercle de la boite à fusible de l'instrument.

Warnung: Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungenersetzen, deren Typ und Nennwert auf den Tafeln neben denSicherungsabdeckungen des Geräts gedruckt sind.

Avvertenza: per garantire protezione contro gli incendi, sostituire i fusibilicon altri dello stesso tipo aventi le caratteristiche indicate sui pannelliadiacenti alla copertura fusibili dello strumento.

Advertencia: Para evitar incendios, sustituir los fusibles por aquellos deltipo y características impresos en los paneles adyacentes a las cubiertas delos fusibles del instrumento.

June 11, 2015, 716004587 Rev. A

警告：為了避免火災，更換保險絲時，請使用與儀器保險絲蓋旁面板上所印刷之相同類型與規格的保險絲。

警告：为了避免火灾，应更换与仪器保险丝盖旁边面板上印刷的类型和规格相同的保险丝。

경고: 화재의 위험을 막으려면 기기 퓨즈 커버에 가까운 패널에 인쇄된 것과 동일한 타입 및 정격의 제품으로 퓨즈를 교체하십시오.

警告: 火災予防のために、ヒューズ交換では機器ヒューズカバー脇のパネルに記載されているタイプおよび定格のヒューズをご使用ください。

Finding fuse types and ratings when that information does notappear on the instrument or device:

Warning: To protect against fire, replace fuses with those of the type andrating indicated in the “Replacing fuses” section of the MaintenanceProcedures chapter.

Avertissement : pour éviter tout risque d'incendie, remplacez toujours lesfusibles par d'autres du type et de la puissance indiqués dans la rubrique"Remplacement des fusibles" du chapitre traitant des procédures demaintenance.

Warnung: Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungenersetzen, deren Typ und Nennwert im Abschnitt "Sicherungen ersetzen" desKapitels "Wartungsverfahren" angegeben sind.

Avvertenza: per garantire protezione contro gli incendi, sostituire i fusibilicon altri dello stesso tipo aventi le caratteristiche indicate nel paragrafo"Sostituzione dei fusibili" del capitolo "Procedure di manutenzione".

Advertencia: Para evitar incendios, sustituir los fusibles por aquellos deltipo y características indicados en la sección "Sustituir fusibles".

警告：為了避免火災，更換保險絲時，應使用「維護步驟」章節中「更換保險絲」所指定之相同類型與規格的保險絲。

警告：为了避免火灾，应更换“维护步骤”一章的“更换保险丝”一节中介绍的相同类型和规格的保险丝。

경고: 화재의 위험을 막으려면 유지관리 절차 단원의 “퓨즈 교체” 절에 설명된 것과 동일한 타입 및 정격의 제품으로 퓨즈를 교체하십시오.

警告: 火災予防のために、ヒューズ交換ではメンテナンス項目の「ヒューズの交換」に記載されているタイプおよび定格のヒューズをご使用ください。

June 11, 2015, 716004587 Rev. A

Electrical symbolsThe following electrical symbols and their associated statements can appear ininstrument manuals and on an instrument’s front or rear panels.

Symbol Description

Electrical power on

Electrical power off

Standby

Direct current

Alternating current

Alternating current (3 phase)

Safety ground

Frame, or chassis, terminal

Fuse

Functional ground

Input

Output

Handling symbolsThe following handling symbols and their associated statements can appear on labelsaffixed to the packaging in which instruments, devices, and component parts areshipped.

Symbol Description

Keep upright!

Keep dry!

June 11, 2015, 716004587 Rev. A

Symbol Description

Fragile!

Use no hooks!

Upper limit of temperature

Lower limit of temperature

Temperature limitation

June 11, 2015, 716004587 Rev. A

Auto•Blend Plus TechnologyAuto•Blend Plus Technology for Ion Exchange, Size Exclusion, and...

Documents

Transcript of Auto•Blend Plus TechnologyAuto•Blend Plus Technology for Ion Exchange, Size Exclusion, and...