Ap

oTo

me

.2 o

n A

xio

Im

ag

er.

Z1

Qu

ick

Op

era

tin

g G

uid

e

1.

Tu

rn o

n M

ain

Sw

itch

( R

ed

la

be

led

A &

B)

2.

Tu

rn o

n a

ll p

ow

er

sup

pli

es

(Sti

cke

r N

o.

1),

wa

it u

nti

l T

FT

fu

lly

lo

ad

3.

Tu

rn o

n H

BO

po

we

r b

ox

(Sti

cke

r N

o.

2),

sw

itch

on

Ap

oT

om

e.2

co

ntr

ol B

ox

(Sti

cke

r N

o.

3)

an

d

Tu

rn o

n t

he

PC

(S

tick

er

NO

.4).

Se

lect

Z

eis

s

use

r

4.

Pre

ss L

oa

d P

osi

tio

n o

n T

FT

to

lo

we

r th

e

ob

ject

ive

s

5.

Pla

ce s

pe

cim

en

sli

de

, se

cure

wit

h s

lid

e h

old

er

8.

Pre

ss

on

TF

T a

ga

in f

or

ob

ject

ive

ba

ck t

o

wo

rkin

g p

osi

tio

n

Sw

itch

ing

On

th

e S

yst

em

& W

ork

ing

wit

h S

pe

cim

en

Notes:

P

ut

on

a d

rop

of

imm

ers

ion

oil

on

to

p l

en

s o

f

63

x a

nd

10

0x

oil

ob

ject

ive

be

fore

use

Mic

rosc

op

e O

pe

rati

on

1.

Do

ub

le c

lick

on

ZE

N 2

01

1 s

oft

wa

re ic

on

on

de

skto

p

to s

tart

th

e s

oft

wa

re

2.

In Z

en

20

11

so

ftw

are

, g

o t

o L

oca

teta

b o

n l

eft

to

ol

are

a t

o e

xam

ine

th

e s

pe

cim

en

on

mic

rosc

op

e a

nd

ide

nti

fy r

eg

ion

of

inte

rest

3.

Pre

ss A

cqu

isit

ion

bu

tto

n t

o c

on

tro

l th

e m

icro

sco

pe

4.

Un

de

r S

ma

rt S

etu

p,

sele

ct r

esp

ect

ive

flu

ore

sce

nt

use

d f

or

the

sa

mp

le w

ith

th

e f

lexi

bil

ity

of

cha

ng

ing

the

co

lor

acc

ord

ing

to

pre

fere

nce

12

3 4

Fo

r fu

rth

er

de

tail

s, k

ind

ly c

on

tact

r.r

am

up

illa

i@ze

iss.

com

1

75.

Go

to

Li

ve

to

pre

vie

w t

he

sa

mp

le (

focu

s o

n y

ou

r sa

mp

le a

nd

do

ne

cess

ary

ad

just

me

nt

to a

chie

ve

be

st i

ma

ge

)

6.

Ad

just

E

xp

osu

re t

ime

fo

r e

ve

ry s

ing

le c

ha

nn

el(

dy

e)

7.

Fo

r C

on

ve

nti

on

al

Ima

gin

g,

Cli

ck

Sn

ap

to

ca

ptu

re a

2D

Im

ag

e

8.

Fo

r O

pti

cal

Se

ctio

nin

g w

ith

Ap

oT

om

e,

mo

ve

Ap

oT

om

e s

lid

er

in m

an

ua

lly

an

d s

et

op

tim

um

pa

ram

ete

r fo

r im

ag

ing

cap

turi

ng

u

nd

er

Ap

oT

om

e

Ta

b b

efo

re g

o t

o L

ive

to

pre

vie

w a

nd

pro

cee

d w

ith

exp

osu

re t

ime

ad

just

me

nt

an

d

Sn

ap

foll

ow

ed

by

Min

/ M

ax

for

ev

ery

sin

gle

Ch

an

ne

l

No

tes:

R

em

em

be

r to

Mo

ve t

he

sli

de

r o

ut

to v

iew

im

ag

e in

soft

wa

re,

rem

ind

er

me

ssa

ge

s a

s b

elo

w,

Cli

ck O

K

Le

ave

Ap

oT

om

e s

lid

er

OU

T f

or

Co

nve

nti

on

al

Flu

ore

sce

nce

Im

ag

ing

(1)

an

d g

en

tly

mo

ve i

n A

po

To

me

Sli

de

r m

an

ua

lly

fo

r O

pti

cal

Se

ctio

nin

g (

2)

F

or

op

tim

um

im

ag

e q

ua

lity

, re

me

mb

er

to c

lick

Min

/

Ma

x u

po

n S

na

p

Fo

r fu

rth

er

de

tail

s, k

ind

ly c

on

tact

r.r

am

up

illa

i@ze

iss.

com

2

56

7

Fo

r fu

rth

er

de

tail

s, k

ind

ly c

on

tact

r.r

am

up

illa

i@ze

iss.

com

3

8Ap

oTo

me

Se

ttin

gs

Fo

r fu

rth

er

de

tail

s, k

ind

ly c

on

tact

r.r

am

up

illa

i@ze

iss.

com

4

3D

Im

ag

ing

Acq

uis

itio

n (

Z-S

tack

Mo

du

le)

1.

Ch

eck

on

ch

eck

bo

x o

f Z

-sta

ck e

xpe

rim

en

t. T

he

Z-S

tack

To

ol b

ar

ap

pe

ars

.

2.

Cli

ck o

n L

ive

bu

tto

n t

o f

ast

sca

n t

he

sp

eci

me

n.

3.

Usi

ng

fo

cus

dri

ve

of

the

mic

rosc

op

e t

o f

ocu

s o

n t

he

hig

he

st p

osi

tio

n o

f th

e s

pe

cim

en

. C

lick

on

S

et

Fir

st

bu

tto

n t

o s

et

the

hig

he

st

po

siti

on

of

the

Z-s

tack

. F

ocu

s in

dif

fere

nt

dir

ect

ion

to

th

e l

ow

est

po

siti

on

of

spe

cim

en

. C

lick

on

th

e

Se

t La

st

bu

tto

n t

o s

et

the

low

est

po

siti

on

. C

lick

ag

ain

on

Liv

e b

utt

on

to

sto

p s

pe

cim

en

sca

nn

ing

(p

rev

en

t sp

eci

me

n f

rom

ble

ach

ing

).

4.

Cli

ck o

n t

he

nu

mb

er

of

slic

e t

hic

kn

ess

aft

er

Op

tim

al

to o

pti

miz

e n

um

be

r o

f sl

ice

s to

ma

tch

th

e o

pti

ma

l Z

-in

terv

al

to t

he

sta

ck

size

. U

nd

er

Op

tim

ize

Se

ctio

nin

g a

nd

ste

pse

ctio

n,

cho

ose

Op

tim

al

bu

tto

n t

o m

atc

h t

he

op

tica

l se

ctio

n f

rom

ea

ch c

ha

nn

el.

5.

Cli

ck o

n S

tart

Ex

pe

rim

en

t b

utt

on

to

sta

rt r

eco

rdin

g o

f th

e Z

-Sta

ck.

6.

Cli

ck

Ga

lle

ry

to v

iew

all

th

e s

lice

s ca

ptu

re w

ith

S

ho

w D

ime

nsi

on

La

be

ls

for

thic

kn

ess

de

tail

s

1

2

3 3

4

4

5

2

1

2

33

4

5

6

6

5

3D

Pro

ject

ion

7

1.

Fo

r 3

D P

roje

ctio

n,

cli

ck 3

D o

n t

he

le

ft p

an

el

of

the

Im

ag

e w

ith

va

rio

us

op

tio

n f

or

3D

vie

w

Fo

r fu

rth

er

de

tail

s, k

ind

ly c

on

tact

r.r

am

up

illa

i@ze

iss.

com

6

To S

av

e I

ma

ge

s

1.

Go

to

Fil

e,

Sa

ve

or

Sa

ve

as,

se

lect

th

e f

old

er

an

d S

av

e t

yp

e

2.

Fo

r 3

D I

ma

ge

s, g

o t

o f

ile

an

d s

ele

ct e

xpo

rt o

pti

on

Fo

r fu

rth

er

de

tail

s, k

ind

ly c

on

tact

r.r

am

up

illa

i@ze

iss.

com

7