Announcements for MCB 110L Office Hours (starting Mon., 28 Jan.) : M12-1 PMJaneGPB 203
description
Transcript of Announcements for MCB 110L Office Hours (starting Mon., 28 Jan.) : M12-1 PMJaneGPB 203
![Page 1: Announcements for MCB 110L Office Hours (starting Mon., 28 Jan.) : M12-1 PMJaneGPB 203](https://reader030.fdocuments.net/reader030/viewer/2022033104/568159a1550346895dc6eff3/html5/thumbnails/1.jpg)
Announcements for MCB 110LOffice Hours (starting Mon., 28 Jan.):
M 12-1 PM Jane GPB 203Tu 2-3 PM Dale GPB 109W 12-1 PM Jesse GPB 201W 12-1 PM Maumita GPB 203F 12-1 PM Joo Eun GPB 203Th 3-4 PM Jeremy 526 Barker
![Page 2: Announcements for MCB 110L Office Hours (starting Mon., 28 Jan.) : M12-1 PMJaneGPB 203](https://reader030.fdocuments.net/reader030/viewer/2022033104/568159a1550346895dc6eff3/html5/thumbnails/2.jpg)
Life Cycle of the Budding Yeast, Saccharomyces cerevisiae
![Page 3: Announcements for MCB 110L Office Hours (starting Mon., 28 Jan.) : M12-1 PMJaneGPB 203](https://reader030.fdocuments.net/reader030/viewer/2022033104/568159a1550346895dc6eff3/html5/thumbnails/3.jpg)
Genetic Nomenclature Conventions for Saccharomyces cerevisiae
Normal (“wild-type”) locus: YFG1
Loss-of-function (hypomorphic) allele: yfg1-1 (point mutation)yfg1-∆1 (null mutation)
Gain-of-function (hypermorphic) allele: YFG1-20
Alteration-of-function (neomorphic) allele: YFG1-54
A normal, hypermorphic or neomorphic allele is dominant overa hypomorphic allele: YFG1/yfg1
Presence and status of an episome or non-Mendelian determinant in brackets: [YCp352], introduced DNA plasmid
[cir+], endogenous 2 m DNA circle [rho-plus = +], functional mitochondrial DNA [psi+], presence of prion form of Sup35 (yeast eRF3) [KIL-k1], endogenous dsRNA viroid encoding secreted toxin
![Page 4: Announcements for MCB 110L Office Hours (starting Mon., 28 Jan.) : M12-1 PMJaneGPB 203](https://reader030.fdocuments.net/reader030/viewer/2022033104/568159a1550346895dc6eff3/html5/thumbnails/4.jpg)
Strain YSA1: MAT fcp1∆::LEU2 TRP1:: fcp1-2ts ade2
his3 leu2 trp1 ura3 [YCp-FCP1, URA3]
The FCP1 gene is essential for S. cerevisiae cell viability.
(mutations in the orthologous human gene product, CTDP1,causes congenital cataracts with facial dysmorphism and neuropathy)
FCP1 [TFIIF-associated Rpo21 carboxy-terminal domain (CTD) phosphatase]
![Page 5: Announcements for MCB 110L Office Hours (starting Mon., 28 Jan.) : M12-1 PMJaneGPB 203](https://reader030.fdocuments.net/reader030/viewer/2022033104/568159a1550346895dc6eff3/html5/thumbnails/5.jpg)
The Concept of a Genetic Cross
e.g., MATa mutation a X MAT mutation b
What can we learn from such crosses?
• Assess whether mutation a is dominant or recessive to thecorresponding WT locus, and assess whether mutation bis dominant or recessive to the corresponding WT locus(dominance test)
• Determine whether mutation a and mutation b are likely to bealterations of the same gene (complementation test)
• Map the relative positions of locus a and locus b by examiningtheir segregation behavior in meiosis (tetrad analysis)
• Generate a potentially useful double mutant (epistasis test)
![Page 6: Announcements for MCB 110L Office Hours (starting Mon., 28 Jan.) : M12-1 PMJaneGPB 203](https://reader030.fdocuments.net/reader030/viewer/2022033104/568159a1550346895dc6eff3/html5/thumbnails/6.jpg)
![Page 7: Announcements for MCB 110L Office Hours (starting Mon., 28 Jan.) : M12-1 PMJaneGPB 203](https://reader030.fdocuments.net/reader030/viewer/2022033104/568159a1550346895dc6eff3/html5/thumbnails/7.jpg)
MATa
MAT
![Page 8: Announcements for MCB 110L Office Hours (starting Mon., 28 Jan.) : M12-1 PMJaneGPB 203](https://reader030.fdocuments.net/reader030/viewer/2022033104/568159a1550346895dc6eff3/html5/thumbnails/8.jpg)
CEN3642 bps642 bps
747 bps747 bps
a haploid:
haploid:
a/ diploid:
![Page 9: Announcements for MCB 110L Office Hours (starting Mon., 28 Jan.) : M12-1 PMJaneGPB 203](https://reader030.fdocuments.net/reader030/viewer/2022033104/568159a1550346895dc6eff3/html5/thumbnails/9.jpg)
MAT2 = YCR039c
MAT1 = YCR040w
MATMATaa11 = no ORF no. = no ORF no. a1
a1
a
a
![Page 10: Announcements for MCB 110L Office Hours (starting Mon., 28 Jan.) : M12-1 PMJaneGPB 203](https://reader030.fdocuments.net/reader030/viewer/2022033104/568159a1550346895dc6eff3/html5/thumbnails/10.jpg)
AB
CD
EFGHIJ
K
MNO
L
P
![Page 11: Announcements for MCB 110L Office Hours (starting Mon., 28 Jan.) : M12-1 PMJaneGPB 203](https://reader030.fdocuments.net/reader030/viewer/2022033104/568159a1550346895dc6eff3/html5/thumbnails/11.jpg)
Systematic name for the FCP1 locus is YMR277w.
Automatically tells you that the ORF encoded bythe FCP1 gene is situated on the right arm of Chromosome XIII and transcribed in the directionaway from CEN13.
![Page 12: Announcements for MCB 110L Office Hours (starting Mon., 28 Jan.) : M12-1 PMJaneGPB 203](https://reader030.fdocuments.net/reader030/viewer/2022033104/568159a1550346895dc6eff3/html5/thumbnails/12.jpg)
MAT2 = YCR039c
MAT1 = YCR040w
MATMATaa11 = no ORF no. = no ORF no. a1
a1
a
a
![Page 13: Announcements for MCB 110L Office Hours (starting Mon., 28 Jan.) : M12-1 PMJaneGPB 203](https://reader030.fdocuments.net/reader030/viewer/2022033104/568159a1550346895dc6eff3/html5/thumbnails/13.jpg)
Available fromCold Spring
Harbor LaboratoryPress
![Page 14: Announcements for MCB 110L Office Hours (starting Mon., 28 Jan.) : M12-1 PMJaneGPB 203](https://reader030.fdocuments.net/reader030/viewer/2022033104/568159a1550346895dc6eff3/html5/thumbnails/14.jpg)
The Concept of a Genetic Cross
e.g., MATa mutation a X MAT mutation b
What can we learn from such crosses?
• Assess whether mutation a is dominant or recessive to thecorresponding WT locus, and assess whether mutation bis dominant or recessive to the corresponding WT locus(dominance test)
• Determine whether mutation a and mutation b are likely to bealterations of the same gene (complementation test)
• Map the relative positions of locus a and locus b by examiningtheir segregation behavior in meiosis (tetrad analysis)
• Generate a potentially useful double mutant (epistasis test)