Animal Procurement, Animal Procurement, Transport, Quarantine

and Health SurveillanceSurveillance

D. HarrisonSupervisor QA LabImport/Export

Laboratory Animal Care and Use Program and Facilities

Import/Export

Animal ProcurementAnimal Procurement

Ho do e get How do we get them in ?them in ?

A d h d And how do we keep them keep them

clean?Laboratory Animal Care and Use Program and Facilities

clean?

Animal ProcurementAnimal Procurement

TOPICSTOPICSTOPICSTOPICS

•• Animal ProcurementAnimal Procurement•• Animal ProcurementAnimal Procurement•• Transporting Lab AnimalsTransporting Lab Animals•• Receiving Quarantine & Receiving Quarantine & •• Receiving, Quarantine & Receiving, Quarantine &

ConditioningConditioning•• Microbiological StatusMicrobiological Status•• Microbiological StatusMicrobiological Status•• Animal Health MaintenanceAnimal Health Maintenance

Laboratory Animal Care and Use Program and Facilities

Animal ProcurementAnimal Procurement

Sooner or later, PI’s will want to order in animals for their studies.

Laboratory Animal Care and Use Program and Facilities

Animal ProcurementAnimal Procurement

Facilities should have aFacilities should have acentralized system in place fort ki d d i ttracking orders and receipts.

We call our system GRANITE

Laboratory Animal Care and Use Program and Facilities

Animal ProcurementAnimal Procurement

Procurement Sources

• Approved Commercial • Approved Commercial Vendors/ SuppliersOth U i iti / • Other Universities/ Collaborators

• Unknown Commercial Vendors

Laboratory Animal Care and Use Program and Facilities

Animal ProcurementAnimal Procurement

An animal order must be placed An animal order must be placed with the Supervisor of the facility that will be receiving the animalsthat will be receiving the animals

Laboratory Animal Care and Use Program and Facilities

Animal ProcurementAnimal Procurement

Laboratory Animal Care and Use Program and Facilities

Animal ProcurementAnimal Procurement

• Reviewed for space available Reviewed for space available in vivarium

• And checked against the current Commercial Vendor current Commercial Vendor listing for approval into the ULAR vivariums

Laboratory Animal Care and Use Program and Facilities

ULAR vivariums

Animal ProcurementAnimal Procurement

A d h k d i t th And checked against the current Commercial Vendor listing for approval into the ULAR vivariums

Laboratory Animal Care and Use Program and Facilities

Animal ProcurementAnimal Procurement

Orders are then sent to the Orders are then sent to the ULAR Animal Desk

• Checked against protocols • Checked against protocols and accounts and entered into Graniteinto Granite.

• Forwarded to purchasing at th R h F d tithe Research Foundation.

Laboratory Animal Care and Use Program and Facilities

Animal ProcurementAnimal Procurement

Non Approved Rodent Orders t t th QAare sent to the QA

Import/Export deskfor quarantine processing.

Laboratory Animal Care and Use Program and Facilities

Animal ProcurementAnimal Procurement

A h lth tA health reportis requestedfrom the facilityfor a yearsworth of testingdata on theanimal facility/room

Laboratory Animal Care and Use Program and Facilities

Animal ProcurementAnimal Procurement

We also checkWe also checkthe type oftransportationto be usedto be used

Laboratory Animal Care and Use Program and Facilities

Transporting Lab AnimalsTransporting Lab Animals

Regulations that Govern Animal Regulations that Govern Animal Transport1. Animal Welfare Act (AWA) 1. Animal Welfare Act (AWA) 2. Public Health Service (PHS) Policy3. The Guide4. CDC5. FDA6. US Dept of Transportation7. US Fish and Wildlife Service8 Oth C t R i t

Laboratory Animal Care and Use Program and Facilities

8. Other Country Requirements

Transporting Lab AnimalsTransporting Lab Animals

AWA Governs-AWA Governs1. Shipping Containers

– SizeSize– Material– # Animals per Container– Food/Water– Temperature Range- Ventilation– Cleanliness

Laboratory Animal Care and Use Program and Facilities

Transporting Lab AnimalsTransporting Lab Animals

AWA Governs – (cont.)AWA Governs (cont.)2. Cargo/Terminal area

Temperature Range Ventilation– Temperature Range- Ventilation– Cleanliness

3 Delivery Vehicles3. Delivery Vehicles– Climate controlled– Cleanliness– Cleanliness

Laboratory Animal Care and Use Program and Facilities

Transporting Lab AnimalsTransporting Lab Animals

Transportation TypesTransportation- Types• Trucking firms

C i l i i• Commercial air carriers• Special equipped

vehiclesvehicles

Laboratory Animal Care and Use Program and Facilities

Receiving AnimalsReceiving Animals

Receipt at the InstitutionReceipt at the Institution• Receiving Room- clean• Check Vehicle shipping animalsCheck Vehicle shipping animals

Laboratory Animal Care and Use Program and Facilities

Receiving AnimalsReceiving Animals

• Disenfect exterior surfaces Disenfect exterior surfaces before entering facility

• Check shipping box condition pp gand verify shipment vs. Granite

Laboratory Animal Care and Use Program and Facilities

Receiving Animalsg

Receipt at the VivariumReceipt at the Vivarium• Pull up Granite, print cage cards • Verify receipt vs animal order• Verify receipt vs. animal orderReceipt at the Room

V if i l b• Verify animal numbers• Examine animals (sex, sick, dead)

Laboratory Animal Care and Use Program and Facilities

Receiving AnimalsReceiving Animals• Transfer Transfer

information to records/cage cards

• Report any problems to the Supervisor

Laboratory Animal Care and Use Program and Facilities

Quarantine of Incoming A i lAnimals

• All animals come into OSU are clean???? • Isolation• Observation, testing, conditioning , g, g• Quarantine Period is dependent on facility

and species.– Rodents 6-8 wks– Non Human Primates 1- 2 months– Others vary by testing and previous health reportsOthers vary by testing and previous health reports

• No experimental procedures (generally)• Animals in quarantine should be handled

Laboratory Animal Care and Use Program and Facilities

Animals in quarantine should be handled after animals of known health status

Quarantine of Incoming A i lAnimals

• Animal TreatmentAnimal Treatment– Health Exams

Diagnostic procedures– Diagnostic procedures• TB tests• Parasitology, bloodwork,Parasitology, bloodwork,microbiology

– Prophylaxis - pinworm op y a s p oFenbendazole feed

– Treatment for existing

Laboratory Animal Care and Use Program and Facilities

gpathogens- found on diagnostics

Quarantine of Incoming A i lAnimals

• Facility DesignFacility Design– Separate from

colony (Isolation)

– Negative pressure pressure environment

WHY??WHY??

Laboratory Animal Care and Use Program and Facilities

Conditioning PeriodConditioning Period

• Allows for acclimatization Allows for acclimatization before study begins

• Can be run at same time as • Can be run at same time as QuarantineSt d i l • Stressed animals hormones (adrenal) which can effect experimental data

Laboratory Animal Care and Use Program and Facilities

Microbiological StatusMicrobiological Status• What does this refer to?What does this refer to?

The types of organisms the animal is carryingy g

Laboratory Animal Care and Use Program and Facilities

Microbiological StatusMicrobiological Status

• Axenic– Means “germ-free”– Born by C-section (sterile)y ( )

• Gnotobiotic (no-toe-by-ah-tic)– Carefully defined microbial floray– All microbes present are known,

usually given to the animal by y g ymouth

Laboratory Animal Care and Use Program and Facilities

Microbiological StatusMicrobiological Status

• Specific Pathogen Free (SPF)– Certain disease (pathogen) Certain disease (pathogen)

eliminated

• Barrier ―Different meanings to facilitiesDifferent meanings to facilities―Known disease status―Sterile or non sterile equipment

Laboratory Animal Care and Use Program and Facilities

q p

Microbiological StatusMicrobiological StatusTrexler ®Trexler ®Systems are used forare used forboth

Laboratory Animal Care and Use Program and Facilities

Microbiological StatusMicrobiological Status• ConventionalConventional

– Raised in normal environment– Number and type of microbes – Number and type of microbes

unknown

Laboratory Animal Care and Use Program and Facilities

Health Surveillance & M i tMaintenance

• Animal Technician is the first line Animal Technician is the first line of defense

• Quality Assurance & Health Monitoring Programg g―Sentinel Program ―Environmental Program―Large Animal Program

• Vaccines/Physicals/ Updated testing• TB Testing

Laboratory Animal Care and Use Program and Facilities

• TB Testing