An Inhalation System to Infect Laboratory Animals.
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Transcript of An Inhalation System to Infect Laboratory Animals.
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An Inhalation System to An Inhalation System to Infect Laboratory AnimalsInfect Laboratory Animals
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Team MembersNick Anders, Kayla Ericson,
Stacey Hoebel, Maja Middleton,Jeff Swift, Ausin Ramme
ClientAdel M. Talaat, Ph.D.
Assistant ProfessorDepartment of Animal and Biomedical Sciences
AdvisorProfessor Willis Tompkins
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Problem StatementProblem Statement•Develop a safe inhalation system for Develop a safe inhalation system for animalanimal infectioninfection•Should be able to infect animals Should be able to infect animals ranging inranging in size from mice, rats, and guinea pigs size from mice, rats, and guinea pigs to to rabbitsrabbits•Should be equipped with pathogen Should be equipped with pathogen escapeescape prevention measures to insure safety prevention measures to insure safety in thein the surrounding environmentsurrounding environment
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Client’s GoalsClient’s Goals• The animal infection study will be used The animal infection study will be used
to:to:– Study pathogenesis of tuberculosisStudy pathogenesis of tuberculosis
•OriginationOrigination•DevelopmentDevelopment
– Develop cure for TB patientsDevelop cure for TB patients•Novel drugsNovel drugs•VaccinesVaccines
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Biological Safety Level Biological Safety Level 33• High risk to individuals, low risk to High risk to individuals, low risk to communitycommunity
• Examples: tuberculosis, yellow fever, Examples: tuberculosis, yellow fever, rabiesrabies
• Safety Precautions:Safety Precautions:– Training for lab workersTraining for lab workers– Protective clothingProtective clothing– Autoclave in work zoneAutoclave in work zone– Non-porous materialsNon-porous materials– Sealed exhaust systemSealed exhaust system– HEPA filtration HEPA filtration – Directional airflowDirectional airflow
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Current Device AvailableCurrent Device Available• Glas-Col companyGlas-Col company
• IdealIdeal– NebulizerNebulizer– FiltersFilters– Air IncineratorAir Incinerator– UV lightUV light
• Prohibitively Prohibitively expensive ($15,000)expensive ($15,000)
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Current Device in UseCurrent Device in Use
• Main chamber: 75 cm x 50 cm x 15 cmMain chamber: 75 cm x 50 cm x 15 cm• Inner chambers: radius 8 cm, height 12 cmInner chambers: radius 8 cm, height 12 cm• Nebulizer, Ethanol Trap, Hepa FilterNebulizer, Ethanol Trap, Hepa Filter• TupperwareTupperware
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Inner ChamberInner Chamber
• TupperwareTupperware• Creates skin Creates skin
infectioninfection• InefficientInefficient• Cannot infect Cannot infect
anything but miceanything but mice• Maximum of 1 mouse Maximum of 1 mouse
per chamber at one per chamber at one time time
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Design SpecificationsDesign Specifications• Dimensions:Dimensions:
– Length: 25 cm – 75 cmLength: 25 cm – 75 cm– Width: 40 cm – 30 cmWidth: 40 cm – 30 cm– Height: 10 cm – 70 cmHeight: 10 cm – 70 cm
• Withstand the Autoclaving ProcessWithstand the Autoclaving Process• Storage at Room TemperatureStorage at Room Temperature• Air Tight StructureAir Tight Structure• Deliver 5 mL of culture in 20 minutesDeliver 5 mL of culture in 20 minutes• Service Life: 50 to 100 UsesService Life: 50 to 100 Uses
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Design 1: Modified Current Design 1: Modified Current DesignDesign
Air output
Animal Cage
Rubber seal
Air input
Clamps
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Modified Current DesignModified Current Design
•StrengthsStrengths– InexpensiveInexpensive– Easy to Easy to
AssembleAssemble– Low MaintenanceLow Maintenance– AutoclavableAutoclavable– More EfficientMore Efficient
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Modified Current DesignModified Current Design
•WeaknessesWeaknesses– Entire Animal Exposed to Entire Animal Exposed to
PathogenPathogen– Inconsistent Air FlowInconsistent Air Flow
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Design 2: Direct Nose Design 2: Direct Nose InfectionInfection
•StrengthsStrengths– Targeted InfectionTargeted Infection– Extremely EfficientExtremely Efficient– Entire Body not ContaminatedEntire Body not Contaminated
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Direct Nose Infection Direct Nose Infection SystemSystem
•WeaknessesWeaknesses– Anesthetization of SubjectAnesthetization of Subject– May cause deathMay cause death– Excessive tubingExcessive tubing
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Final Design: Final Design: • Dual Chamber Dual Chamber
SystemSystem• 4 Input Air Flow4 Input Air Flow• Latex Flange Latex Flange
around Neckaround Neck• Three Species Three Species
Specific Inner Specific Inner ChambersChambers
Air input
animal
Air input
Animal head
Air output
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Future WorkFuture Work•Construction of inner stalls for Construction of inner stalls for
easy maintenanceeasy maintenance•Restraint systemsRestraint systems•FansFans
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