An in Vitro Diagnostic Assays determine by using of Beer Lambert's Law - MATHANKUMAR S
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Transcript of An in Vitro Diagnostic Assays determine by using of Beer Lambert's Law - MATHANKUMAR S
A = ε b c
A Æ absorbance (-)
ε Æ molar absorbtivity with units of L mol-1
cm-1
b Æ path length of the sample (cuvette)
c Æ Concentration of the compound in
solution, expressed in mol L-1
•Amount of light that a sample absorbs.
•Beam of monochromatic light
•Measuring the intensity of light reaching a
detector
• Deviations in absorptivity coefficients at
high concentrations(> 0.01M) due to
electrostatic interactions.
• Scattering of light due to particulates in
the sample
• Fluoresecence or phosphorescence of the
sample
• Changes in refractive index at high analyte
concentration
• Non-monochromatic radiation
ADC Signal Processor
Lamp
CHAMBER
Sample Sensor & PRE-AMP
Absorbance= log 10 ( VR / Vs ) Where VR = Reference voltage
Vs = Sample voltage
Derivation