AimPlex Introduction LinkedIn

1

For Research Use Only. Not for use in diagnostic procedures.

Your Resource for Customized Multiplex Immunoassay Panels

II. Assay Principal, offering and benefits of AimPlex Multiplex Immunoassay Solutions

III. Equipment needed for AimPlex Assays

VI. AimPlex Multiplex Immunoassays by Area of

Research

I. Multiplex immunoassays and biomedical research

2

Your Resource for Customized Multiplex Assay Panels

Multiplex Immunoassays

and Biomedical Research

3


Up to 24-plex: differentiated by size and levels of fluorescence intensity

◦ The bead classification dye can be excited by a blue, green or red laser. The maximum emission is 700 nm.

Analyzed on a conventional flow cytometer

4


xMap*

R&D Systems

Millplex* Bio-

Plex*

Luminex* Platform

CBA

Flow Cytometers

AimPlex

#Trademarks are the property of their respective owners

5


Example: Th1/Th2/Th17

6


7


Ref: http://www.slideshare.net/nayeemdaisu/6-cytokine?next_slideshow=5

http://www.slideshare.net/nayeemdaisu/6-cytokine?next_slideshow=5



8


Ref: http://www.slideshare.net/nayeemdaisu/6-cytokine?next_slideshow=5




9

Oncology Immunology

Metabolic Cardiovascular

diseases

Stem cells

Cellular therapy Transplantation

Cell signaling Apoptosis

Less sample volume

Higher throughput

Higher content

Less time Less cost


Neurodegeneration

Pituitary endocrine

Inflammatory / immune response

Lipid cell metabolism

Endocrie

Bone metabolism

Neuroendocrinology

Thyroid

CVDs

Intestinal hormone Antibody subtyped

Skin

Oncology Cell Signaling

10


Life Science Research

◦ Universities

◦ Research centers

◦ Medical centers

Drug Discovery

◦ Pharmaceutical

◦ Biopharmaceutical

11


Clinical Research

• CROs

• Hospitals

• Medical centers

Flow cytometer areas

of applications

• Immunology

• Cell Biology

• Cancer research

• Infectious disease

Assay Principal, Offering and Benefits of AimPlex Multiplex

Immunoassay Solutions

12


Up to 24-plex (12 analytes on 2 bead sizes)

Applicable to a flow cytometer equipped with a

488nm (blue) laser.

13

AimPlex bead-based “sandwich” immunoassays


Fluorescence dyed Bead – Capture Ab (cAb) - Analyte – Detection Ab (dAb)

14

Beads classified by size and fluorescent intensities (excited by a 488, 531or 630 nm laser, Em-Max=700nm)

PE (575nm) as a reporter conjugated to dAb

R1

R2

Gate on R2 (larger beads) R1: 4 micron beads R2: 5 micron beads

IL-1b

IL-6

IL-12P70

IL-8 “PE-C

y5

”

“PE-C

y5

”

FSC PE


Sample types: cell culture supernatant, serum, plasma, bodily fluid and cell/tissue lysates

Sensitivity (LOD): Majority of the assays <10 pg/mL

Quantitation range:

◦ LLOQ: Majority of the assays <20 pg/mL

◦ ULOQ: Majority of the assays >5,000 pg/mL

Standard dose recovery: 70-130%

Precision

◦ Intra-assay CV: <10%

◦ Inter-assay CV: <20%

Cross-reactivity of analytes in a Panel: negligible

Sample volume: 15 µL

15

LOD: Lower limit of detection LLOQ: Lower limit of quantitation ULOQ: Upper limit of quantitation


Multiplexing allows for:

◦ Less sample (15 µL)

◦ Less assay time (~2 hours) and cost of supplies and labor

Consistent and superior assay performance

Flexible assay panels

◦ Mix and match from a given standard Multiplex Group

◦ Or customize to match your research objectives

339 assays available (as of 7/27/2015) and more being developed

Pre-mixed panels are ready to use right “out-of-the-box”

Use an existing flow cytometer. There is no need to purchase and maintain another expensive instrument

AimPlex provides a “lowest price” guarantee

16


17


18

Data obtained on a Beckman’s CyAn

Size 4 beads (S4): 4 microns

Size 5 beads (S5): 5 microns

FSC

SSC


19

Size 4 beads (S4)

Size 5 beads (S5)

Data obtained on a Sony EC800 with 488/640 lasers

“APC-like” Emission (665/30nm)

FSC

S5P1 S5P12 S5P2 S5P11

S4P1 S4P12 S4P2 S4P11


20

Data obtained on a Sony EC800 with 488/640 lasers

“APC-like Emission” (665/30nm)

“PE-C

y5

” (7

00

/7

5)

S4P1

S4P3

S4P2

S4P4

S4P5

S4P6

S4P7

S4P8

S4P9

S4P10

S4P11

S4P12

Size 4 beads (S4)

S5P1

S5P2

S5P3

S5P4

S5P5

S5P6

S5P7

S5P8

S5P9

S5P10

S5P11

S5P12

Size 5 beads (S5)


21

Analytes LOD (pg/mL) LLOQ (pg/mL)

Human IFNγ <3 <10

Human IL-2 <3 <10

Human IL-4 <1 <3

Human IL-6 <5 <10

Human IL-10 <2 <5

Human IL-17A <3 <10

Human TNFα <1 <2

Analytes LOD (pg/mL) LLOQ (pg/mL)

Mouse IFNγ < 0.5 < 2

Mouse IL-2 < 2 < 5

Mouse IL-4 < 0.5 < 2

Mouse IL-6 < 3 < 10

Mouse IL-10 < 2 < 5

Mouse IL-17A < 1.5 < 5

Mouse TNFα < 0.5 < 2

*Analyzed on a Sony EC800 flow cytometer


IL-4

IL-6

IL-10 TNFa

IL-4

IL-6

IL-10 TNFa

5,000 pg/mL 1,667 pg/mL 556 pg/mL 185 pg/mL

62 pg/mL 21 pg/mL 7 pg/mL 0 pg/mL

22


IL-2

IFNg

IL-17A

IL-2

IFNg

IL-17A

5,000 pg/mL 1,667 pg/mL 556 pg/mL 185 pg/mL

62 pg/mL 21 pg/mL 7 pg/mL 0 pg/mL

23


24


Data Analysis FCAP array* 3.0 Software

Generate standard curves

Calculate concentrations

Generate reports (Excel or PDF)

#Trademarks are the property of their respective owners 25


26

Cell culture supernatant samples from human PBMCs treated and untreated (control) with PMA + Ionomycine (PMA+Iono), LPS+R848, R848 and LPS were tested using AimPlex human cytokine assays.

Control

P + I

CD3

LPS



18-Plex Panel

27

Cell culture supernatant samples from human PBMCs treated with various reagents were assayed by using AimPlex human Th1/2/17 7-Plex assays and a commercially available Luminex multiplex assay kit.

AimPlex Assays were analyzed on a BD FACSCalibur*



28

Serum samples from mice, treated with or without (Control) 5mg/kg of lipopolysaccharides (LPS) for 6 hours, were tested using an AimPlex mouse 16-plex panel. (Data from Dr. ZX Liu's lab at the University of Southern California)

*Analyzed on a BD FACSCalibur


29

Serum and blood clot lysate samples of mice, treated with or without (Con) LPS, were tested using AimPlex mouse cytokine assays. This experiment is important indicating that besides serum, blood clots (after lysed with AimPlex lysis buffer) can be used for measurements of many blood cytokines, thus resulting in savings of precious serum samples that can be saved for other assays



AimPlex Main Assays

Cytokines Chemokines Inflammation Growth factors Angiogenic factors Adipokine Soluble receptors/CD antigens Tumor Others

Species: • Human • Mouse • Rat • NHP (non-human primate) • Canine • Porcine (coming soon)

30


Mix and match from the same groups to create your own panel

339 assays available (as of 5/23/15)

Ready-to-use, premixed Ags, cAb-beads and dAbs

More than 20 premixed kits available

Available in both 96-tests and 32-tests

Customizing available

Premixed Lyophilized

31


Sample Types

• Serum

• Plasma

• Cell culture supernatant

• Cell lysate

• Tissue lysate

• Saliva

• BALF

• Pleural fluid

• Peritoneal fluid

• Synovial fluid

• Cerebrospinal fluid

• Blood clot

Common Less Common

32


Name Description Size

Human Th1/Th2 10-plex IFNgamma, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, TNFalpha 96T/32T

Human Th1/Th2/Th17 7-plex IFN-gamma,IL-10/CSIF,IL-17A/CTLA-8,IL-2,IL-4,IL-6,TNF-alpha 96T/32T

Human Th1/Th2/Th17 14-plex IFNgamma, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-17F,

IL-22, TNFalpha, TNFbeta 96T/32T

Human Th1/Th2/Th17 18-plex GM-CSF, IFNgamma, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-12p70,

IL-17A, IL-17F, IL-22, IL-33, TNFalpha, TNFbeta, TSLP 96T/32T

Human Inflammation 7-Plex IL-1b,IL-1RA,IL-8,IL-6,IL-10,TNFalpha,IL-12p70 96T/32T

Human Inflammation 11-Plex IFNgamma, IL-1alpha, IL-1beta, IL-6, IL-8, IL-10, IL-12p70, IL-27, IP-10, MCP-1,

TNFalpha 96T/32T

Human Inflammation 18-Plex IFNgamma, IL-1alpha, IL-1beta, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A,

IL-27, IL-31, IL-33, IP-10, MCP-1, MIP-1alpha, MIP-1beta, and TNFalpha 96T/32T

Human Chemokine 7-Plex Eotaxin,IL-8,RANTES,MIG,MCP-1,MCP-3,IP-10 96T/32T

Human CD8+ T Cell Related

Cytokines, 16-plex GM-CSF, Granzyme B, IFNgamma, IL-2, IL-4. IL-5, IL-6, IL-10, IL-13, IL-15,

MIP-1alpha, MIP-1beta, RANTES, sCD137, sFasL, TNFalpha 96T/32T

Human IGF I, IGF II 2-plex IGF I,IGF II 96T/32T

33


Name Description Size

Mouse Th1/Th2/Th17 10-plex GM-CSF, IFNgamma, IL-1alpha, IL-2, IL-4, IL-5, IL-6, IL-10,

IL-17A and TNFalpha 96T/32T

Mouse Th1/Th2/Th17 7-plex IFN-gamma,IL-10/CSIF,IL-17A/CTLA-8,IL-2,IL-4,IL-6,TNF-alpha 96T/32T

Mouse Th1/Th2/Th17 14-plex IFNgamma, IL-1alpha, IL-1beta, IL-2 , IL-4, IL-5, IL-6, IL-10, IL-12p70,

IL-13, IL-17A, IL-21, IL-22 and TNFalpha 96T/32T

Mouse Th1/Th2/Th17 18-plex GM-CSF, IFNgamma, IL-1alpha, IL-1beta, IL-2 , IL-4, IL-5, IL-6, IL-10,

IL-12p70, IL-13, IL-17A, IL-21, IL-22, IL-23, KC, TNFa and TSLP 96T/32T

Mouse Inflammation 8-Plex IL-1a,IL-6,IL-10,KC,MCP-1,IFNgamma,TNFalpha,IL-12p70 96T/32T

Mouse Inflammation 11-Plex IFNgamma, IL-1alpha, IL-1beta, IL-6, IL-10, IL-12p70, IL-23, IP-10, KC, MCP-

1, TNFalpha 96T/32T

Mouse Inflammation 17-Plex IFNgamma, IL-1alpha, IL-1beta, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-

23, IP-10, KC, MCP-1, MIP-1alpha, MIP-1beta, RANTES , TNFalpha 96T/32T

Mouse Chemokine 7-Plex Eotaxin,KC,RANTES,MIG,MCP-1,MCP-3,IP-10 96T/32T

Rat Th1/Th2/Th17 7-plex IFN-gamma,IL-10/CSIF,IL-17A/CTLA-8,IL-2,IL-4,IL-6,TNF-alpha 96T/32T

NHP Th1/Th2/Th17 7-plex IFN-gamma,IL-10/CSIF,IL-17A/CTLA-8,IL-2,IL-4,IL-6,TNF-alpha 96T/32T

NHP IGF I, IGF II 2-plex IGF I IGF II 96T/32T

34


An unmet need in the current multiplex immunoassay market

• Many experiments require custom assay panels

• Expensive to produce by large companies due to time-consuming documentation workload and review process

• Other multiplex assay providers, if offering custom panels, usually charge extra and/or require a minimum order of analytes or plates

AimPlex provides a solution for this unmet need: • No extra charge for custom panels

• Customer only needs to order one 96-well plate

• Custom premixed beads as well as custom premixed detection Abs (dAbs) at no extra charge

• >80% of AimPlex orders are custom panels.

35


A more comprehensive menu

Competitive pricing: priced 20% less for the same assays

Expedited custom panel development

More ‘user-friendly’ assay kits with custom premixed panels at NO EXTRA CHARGE

More flexible assay panels

36


Use an existing flow cytometer: no need to purchase and maintain another expensive instrument

Higher throughput with a HTS microplate loader (10-15min)

◦ It takes >45min to read a 96-well plate on a Luminex 100/200

Much less chance of clogging especially for complex matrix such as serum and plasma

◦ Flow cytometers are designed for cells (many are much larger than the 4 and 5 micron beads).

AimPlex will make custom panels

37


Examples：7 analytes, 40 samples

AimPlex ELISA

Sample Volume 15uL 350-700ul

96-well Plates 1 plate 7 plates

Time ~4 hrs ~47 hrs

Assay Range 2-5000pg/mL 2-1500pg/ml

Reaction System One Seven

38


Equipment Required for

AimPlex Immunoassays

39


40


Simplifies bead washing steps

Much easier to use and less expensive than a vacuum manifold from Millipore or Pall

List price: $498

40% discount for first time customers ordering AimPlex kits

41


42


Flow Cytometer Scatter Parameter

Bead Clustering Parameter

Reporter Parameter

Color Compensation

BD FACSCalibur* (single laser)

FSC FL3 (670nm) FL2 (575nm) Yes

BD FACSCalibur* (dual laser)

FSC FL4 (660nm) FL2 (575nm) No

Accuri C6 FSC FL4 (660nm) FL2 (575nm) No

BD FACSCanto*, BD FACSCanto* II

FSC FL4 (660nm) FL2 (575nm) No

BD FACSArray* FSC Red (660nm) Yellow (575nm) No

BD FACSVerse* (2-laser, 6-color)

FSC APC (660nm) PE (586nm) No

BC CyAn ADP* FS FL8 (660nm) FL2 (575nm) No

BC FC500* FS Red2 (660 nm) FL2 (575nm) Yes

Sony EC800* FS FL4 (700nm) FL2 (575nm) Yes

43


NOTE: Both Areas and Heights of the FL parameters should be collected.


AimPlex Multiplex Immunoassays by Area of Research

44


Research Area Analytes

Dermatitis sCD30, sICAM-1, IL-4, IL-5, IL-13, TNF-alpha, RANTES, IP-10

CVDs Adiponectin, sCD40L, CRP, sE-selectin, sICAM-1, IL-1beta, IL-6, IL-8, , MCP-1, MPO, PAI-1, sP-selectin, TNF-alpha, t-PA, sVCAM-1

Diabetes Adiponectin, CRP, sICAM-1, IFN-alpha, IFN-gamma, Iso-Insulin, IL-1beta, IL-6, IL-8, IL-10, , Leptin, MCP-1, MIP-1alpha, MIP-1beta, NGF, PAI-1, Resistin, TNF-alpha, TNF-beta, t-PA

Growth and Differentiation

G-CSF, IFN- alpha, IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, , IL-23, MCP-1, NGF, TNF-alpha

Immune Regulation

sCD30, sCD40L, IL-4, IL-6, IL-10, IL-12p70, IL-13, IL-23, IFN-alpha, IFN-gamma, TNF-alpha

Inflammation

sCD30, sCD40L, CRP, sE-selectin, G-CSF, sICAM-1, IFN- alpha, IFN-gamma, IL-1beta, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, , IL-23, MCP-1, MIG, MIP-1alpha, MIP-1beta, sPECAM-1, sP-selectin, TNF-alpha, sTNF-R1, sVCAM-1

Multiple Sclerosis sCD30, sICAM-1, IFN-gamma, IL-4, IL-8, IL-10, IL-12p70, IL-17A, MCP-1, MIP-1beta, TNF-alpha, TNF-beta, sVCAM-1

45


Obesity Adiponectin, sCD40L, CRP, sICAM-1, IL-6, , Leptin, MCP-1, MPO, NGF, OPG, PAI-1, Resistin, TNF-alpha, sTNF-R1

Pain sICAM-1, IL-1beta, IL-6, IL-8, IL-10, IL-12p70, IFN-gamma, MCP-1, MIP-1 alpha, MIP-1beta, NGF, TNF-alpha, sVCAM-1

Preeclampsia Adiponectin, IFN-gamma, IL-4, IL-6, IL-8, IL-10, IL-12p70, , TNF-alpha

Rheumatoid Arthritis

sCD30, sE-selectin, sICAM-1, sICAM-3, IFN-alpha, IL-1beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, , IL-23, MCP-1, MIP-1alpha, sP-selectin, TNF-alpha, sVCAM-1

Sepsis

CRP, sE-selectin, IL-1beta, IL-4, IL-6, IL-8, IL-10, IL-12p70, , MCP-1, MIP-1 alpha, MIP-1beta, PAI-1, TNF- alpha, sVCAM-1), Septic shock (IL-1beta, IL-4, IL-6, IL-8, IL-10, IL-13, IFN-gamma, G-CSF, MCP-1, PAI-1, TNF-alpha

Systemic lupus erythematosus

sCD30, sCD40L, IFN- alpha, IL-6, IL-10, IL-12p70, IL-13, , NGF, TNF-alpha, sVCAM-1

Tumor necrosis factor / tumor necrosis factor

receptor

sCD30, sCD40L, NGF, OPG,TNF-alpha, TNF-beta, sTNF-R1

Transplantation CRP,ICAM and VCAM,, sVCAM-1,sCD30, sICAM-1, IFN- alpha, IFN-gamma, IL-1beta, IL-2, IL-4, IL-6, IL-10, , MCP-1, MIG, MIP-1 alpha, TNF-alpha

Cancer sCD30, sCD40L, IFN-gamma, IL-1beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-23, MCP-1, MIP-1 alpha, MIP-1beta, NGF,TNF-alpha, TNF-beta,sICAM-1, sICAM-3, sE-selectin, sP-selectin and sVCAM-1

46


Available Assays*

Human Mouse Rat NHP

YSL Bioprocess Development Co. 2265 E. Foothill Blvd. Pasadena, CA 91107 Tel: 1-866-618-8932; Fax: 626-604-5228 Email: [email protected]

*Please visit www.yslbio.com for the most up-to-date assay list.

Canine

6Ckine/CCL21 CD56/sNCAM-1/sCD56 IL-13 MIP-1 beta/CCL4 6Ckine/CCL21/Exodus-2 IL-2 beta-NGF/NGFB Eotaxin/CCL11 Adiponectin CD62E/sE-selectin/ELAM1 IL-15 MIP-1δ/CCL15/SCYA15 Activin A IL-20/IL-10D CD254/RANKL/TNFSF11 IFNgamma AFP CD62L/sL-selectin/LECAM1 IL-16/LCF MIP-3α/CCL20 BDNF IL-21 Eotaxin/CCL11 IL-1beta

ANGPT-1/Angiopoietin-1 CD62P/sP-selectin/LECAM-3 IL-17A/CTLA-8 MIP-3β/CCL19/SCYA19 BLC/CXCL13 IL-22 G-CSF/CSF-3 IL-1RA BCA-1/CXCL13 CD66a/sCEACAM-1/Cea-1 IL-17C MPIF-1/CCL23 BRAK/CXCL14/MIP-2γ IL-23p19 GM-CSF/CSF-2 IL-2 BDNF CD95/sFas/TNFRSF6 IL-17F MPO BTC/Betacellulin IL-25/IL-17E GRO alpha/KC/CXCL1 IL-4

beta-NGF/NGFB CRP/C-Reactive Protein IL-1alpha/IL-1F1 NAP-2/CXCL7 CD10/Neprilysin/sNEP IL-28A/B/IFNlambda 2/3 ICAM-1/CD54 IL-6

BMP-2 CTACK/CCL27 IL-1beta OPG/Osteoprotegerin CD105/Endoglin IL-3 IFNgamma IL-8/CXCL8

BMP-4 CXCL16 IL-1RA OPN/Osteopontin CD106/sVCAM-1 IL-33/NFHEV/DVS27 IL-1 alpha/IL-1F1 IL-10/CSIF

BRAK/CXCL14 Cystatin C IL-2 OSM/Oncostatin M CD119/sIFNγR1/IFNγRa IL-4 IL-1 beta/IL-1F2 IL-12p70

CCL28/MEC EGF IL-20/IL-10D PAPP-A CD120a/sTNFRI IL-5 IL-10/CSIF IL-17A/CTLA-8

CD105/Endoglin ENA-78/CXCL5 IL-21 PARC/CCL18 CD120b/TNFRII IL-6 IL-12p40 IP-10/CXCL10

CD106/sVCAM-1 Eotaxin/CCL11 IL-22/IL-TIF PDGF-AB CD121a/IL-1RI IL-7 IL-12p70 MCP-1/JE/CCL2

CD120a/sTNFRI/TNFRSF1A Eotaxin-2/CCL24/MPIF-2 IL-23p19 PF4/CXCL4 CD14/sCD14 IL-9 IL-13 MCP-3/MARC/CCL7

CD120b/TNFRII/TNFRSF1B Eotaxin-3/CCL26 IL-24 RAGE/AGER/sRAGE CD153/sCD30L/TNFSF8 IP-10/CXCL10 IL-15 MIG/CXCL9

CD121a/sIL-1RI/sCD121a FGF basic/FGF-2/HBGH-2 IL-27 RANTES/CCL5 CD154/sCD40L/TRAP Leptin/LEP IL-17A/CTLA-8 RANTES/CCL5

CD121b/IL-1RII FGF-1/FGF-acidic IL-28A/IFNlambda 2 Resistin/ADSF CD266/sTWEAK-R/Fn14 LIF IL-2 TNFalpha

CD126/IL-6Rα/IL-6R alpha FGF-19 IL-29/IFNlambda 1 SCF/MGF/KITLG CD27/sTNFRSF7 LIX/GCP2/CXCL5 IL-4

CD130/Gp130 Flt-3 Ligand/Flt-3L/Flk-2L IL-3 SDF-1/CXCL12 CD309/sVEGFR2/KDR MCP-1/JE/CCL2 IL-5

CD137/s4-1BB/TNFRSF9 Fractalkine/CX3CL1 IL-31 SLPI CD54/sICAM-1 MCP-3/MARC/CCL7 IL-6

CD14 GASP-1/WFIKKNRP IL-32 ST2/IL-1R4 CD62E/sE-selectin MCP-5/CCL12 IP-10/CXCL10

CD153/CD30 Ligand GCP-2/CXCL6 IL-33 Survivin/BIRC5 CD62L/sL-selectin M-CSF/CSF-1 Leptin

CD154/CD40L/sCD40L G-CSF/CSF-3 IL-34 TARC/CCL17 CD62P/sP-selectin MDC/CCL22/ABCD-1 LIX/GCP2/CXCL5 IFNγ CD166/sALCAM/sCD166 GDF-15/MIC-1/PTGFB IL-4 TECK/CCL25 CTACK/CCL27 MIG/CXCL9 MCP-1/JE/CCL2 IL-2 CD178/sFas Ligand/TNFSF6 GM-CSF/CSF-2 IL-5 TGFalpha CXCL16 MIP-1 alpha/CCL3 MCP-3/MARC/CCL7 IL-6 CD244/sSLAMF4/2B4/NAIL Granzyme B IL-6 TGF-beta 1 Eotaxin/CCL11 MIP-1beta/CCL4 MIP-1alpha/CCL3 IL-8/CXCL8 CD25/IL-2RA GROα/CXCL1/MGSA IL-7 TIM-1/KIM-1/HAVCR FGF1/FGFacidic/HBGF1 MIP-1γ/CCL9/CCL10/MRP2 MIP-2/GRO beta/CINC3 IL-10/CSIF CD254/sRANKL/TNFSF11 HCC-1/CCL14 IL-8/CXCL8 TIMP-1 FGF2/FGFbasic/HBGH2 MIP-2/GRO beta/CINC3 RANTES/CCL5 IL-12p40 CD256/APRIL/TNFSF13 HCC-4/CCL16 IL-9 TIMP-2 FGF-9/HBGF-9/GAF MIP-3α/CCL20 SDF-1/CXCL12 IL-17A CD257/BlyS/BAFF HGF/Hepatopoietin A Insulin TNFalpha G-CSF/CSF-3 MIP-3β/CCL19/ELC TNFalpha MCP-1/JE/CCL2 CD258/sLIGHT/TNFSF14 I-309/CCL1 IP-10/CXCL10 TNF-beta/TNFSF1/LTA GM-CSF/CSF-2 OPG/Osteoprotegerin VCAM-1/CD106 SCF/MGF/KITLG

CD263/sTRAIL-R3/DcR1 IFNalpha 2 I-TAC/CXCL11 TPO/Thrombopoietin Gro alpha/KC/CXCL1 OSM/Oncostatin M VEGF TNFα

CD270/sHVEM/TNFRSF14 IFNbeta Leptin/LEP TSLP HGF/HPTA/SF RAGE/sRAGE/AGER

CD273/sB7-DC/PD-L2 IFNgamma LIF VEGF-A IFNgamma RANTES/CCL5 CD274/sB7-H1/PD-L1 IGF1 Lipocalin-2/NGAL VEGF-C IL-10/CSIF sCD127/IL-7R/IL-7Rα CD279/sPD-1/sCD279 IGF2 LL-37 VEGF-D IL-11 sCD25/IL-2Rα/Ly43 CD30/TNFRSF8 IGFBP-1 MCP-1/JE/CCL2 VEGFR1/Flt-1/sVEGFR1 IL-12/IL-23 p40 sCD254/RANKL CD309/sVEGFR2/Flk-1 IGFBP-3 MCP-2/CCL8/SCYA8 IL-12p70 sCD257/BlyS/BAFF CD31/sPECAM-1 IGFBP-5 MCP-3/MARC/CCL7 IL-13 TARC/CCL17 CD324/sE-Cadherin/CDH1 IGFBP-6 MCP-4/CCL13/SCYA13 IL-15 TGF-beta 1 CD326/sEpCAM/MK-1/TROP1 IGFBP-7 M-CSF IL-17A/CTLA-8 TIMP-1 CD353/sBLAME/SLAMF8 IL-10/CSIF MDC/CCL22/SCYA22 IL-17F TNFalpha CD40/sTNFRSF5/sCD40 IL-11 MIF IL-1alpha/IL-1F1 TSLP CD50/sICAM-3/sCD50 IL-12/IL-23p40 MIG/CXCL9 IL-1beta/IL-1F2 VEGF

CD54/sICAM-1 IL-12p70 MIP-1 alpha/CCL3 IL-1RA VEGFR1/Flt-1

Rev. 7/28/2015

mailto:[email protected]

http://www.yslbio.com/

Which sample types are suitable for AimPlex Immunoassays?

◦ Human, non-human primate, mouse and rat cell culture supernatant, serum, plasma, cell/tissue lysate and other body fluids such as saliva, BALF, pleural fluid, peritoneal fluid, synovial fluid and cerebrospinal fluid.

What is the maximum multiplex level?

◦ Up to 24-plex.

What flow cytometer can be used?

◦ Any flow cytometry analyzer equipped with a 488nm laser.

Does it need a plate loader?

◦ No. Process the assay on a filter plate. Transfer to sample tube and run on a flow cytometer without a plate loader.

Can I use a Luminex* to analyze AimPlex assays?

◦ No.

Any other equipment needed?

◦ AimPlex EzPrep Filter Plate Washer (PN: VM1001)

◦ Plate shaker.

Can I create my own multiplex panel?

◦ Yes, by ordering multiple “Single-Plex” kits within a group. Custom premixed panels are also available for 6 or more analytes in a panel at no extra charge (allow 15 business days for delivery).

If the assay/panel I want is not available, what should I do?

◦ We offer custom premixed multiplex panels as well as custom assay development for analytes not currently available. We will work with you to design panels and assays. Please contact us for pricing, lead time and details for those offerings.

How to analyze the data?

◦ We recommend using SoftFlow’s FCAP Array* V3.

How to place an order?

◦ Complete an AimPlex ordering form and email ([email protected]) or fax (626-604-5228) for a quote so you can place an order accordingly.


48


AimPlex Introduction LinkedIn

Documents

Transcript of AimPlex Introduction LinkedIn