Advanced methods of protein microsequence analysis
1
Gen. Pharmac. Vol. 19, No. 4, pp. 637~538, 1988 0306-3623/88 $3.00 + 0.00 Printed in Great Britain. All rights reserved Copyright © 1988 Pergamon Press plc BOOK REVIEWS Manual of Pharmacologic Calculations with Computer Programs--R. J. Tallarida and R. B. Murray. 2nd edn, 297 pp. 1987. Springer, New York. $35. If you do many calculations of the following type: dosage and concentrations; drug stock solutions; graded dose response; quantal dose response; relative potency; dissociation con- stants of agonists, partial agonists, perturbation methods; pA2 analysis; enzyme kinetics; drug decay; pharmaco- kinetics; statistical analysis of results (whole range of tests); then this book will be useful to you. It provides details of how to do these calculations on a micro and the software programs in Basic for each calculation so you can copy these programs and run them on your micro. It is also possible to purchase the programs on a disc (Version 4) and the original purchase price of this book is deducted from the disc price. The programs will run on MS-DOS computers (IBM and compatibles) and Tandy/Radio Shack TRS-80 models. If you already have an older version of the disc it can be credited to you and replaced by the new more complete version. This system will be very useful in all pharmacological teaching and research departments. Cocaine, Clinical and Biobehaviourai Aspects---Edited by S. Fisher, A. Raskin and E. H. Uhlenhuth. 256 pp. 1987. Oxford University Press. $24.95. Opiates like most substances are neutral in themselves but have good or bad effects depending on their application. Unfortunately the bad effects of cocaine are now dominant and this multi-authored volume describes the scientific basis for the abuses. The chapters are; the history of the use of cocaine; the pharmacological mechanisms of action; neur- onal bases for the hedonistic effects of cocaine and opiates; neural substrates for cocaine and opiate reinforcement; chronic cocaine administration, sensitization and kindling effects; cocaine abuse treatment research; clinical issues in cocaine abuse; coca and other psychoactive plants, magico- religious roles in primitive societies in the New World. The chapters are well written and provide a valuable scientific study of the present problems and research. Advanced Methods of Protein Microseqnence Analysis-- Edited by B. Wittmann-Liebold, J. Salnikow and V. Erdmann. 423 pp. 1986. Berlin. DM 198, Although recombinant DNA technique is very useful in determining protein structure, the direct sequencing of a protein can often provide a characterization of the poly- peptide structure. Protein fragmentation can be used to determine functional domains and active sites. It is estimated that in eukaryotic cells at least one third of all proteins may have the N or C terminus of the chain blocked. Sequence analysis also can provide information about the secondary and tertiary structure. Following a FEBS advanced course on microsequencing and an international symposium on sequencing, the editors asssembled the methods they are published in this multi-authored book. The chapters are; separation and AA analysis of proteins and peptides for microsequencing studies; manual and solid phase micro- sequencing methods; gas phase and radio-sequence analysis; phenylthiohydantoin identification, on-line detection, sequence control and data processing; analysis of cysteine residues in proteins; methods of analysing protein conform- ation; strategies and specific examples of sequencing proteins and peptides: alternative sequencing techniques for elucid- ation of peptide and protein sequencing. Full practical details are given how to carry out each stage, how much material to use, what chemicals and equipment, the type of results obtained. The book will be very useful in all laboratories interested in sequencing. Principles and Models of Biological Transport--Morton H. Friedman. 260 pp. 1986. Springer, Berlin. DM 165. This test is aimed at the advanced undergraduate/graduate level and deals with equilibrium thermodynamics; free diffu- sion; facilitated diffusion; active transport; nonequilibrium thermodynamics; models of transport across cell mem- branes; single cells (erythrocytes, nerve, muscle); epithelial transport (small intestine, kidney, glomerular filtration, proximal tubule, loop of Henle, distal tubule, collecting tubule) gas transport. Its approach is rigorous with accent on the physical and mathematical explanations and gives the engineering attitude to biological transport. Calcium in Muscle Activation: A Comparative Approach~ J. C. Ruegg. 300 pp. 1986. Springer, Berlin. DM 198. Calcium plays a key role in controlling muscle contraction and relaxation and this single author work provides an excellent summary of the molecular basis of muscle move- ment. The chapters are: muscle excitation and contraction; the sarcoplasmic reticulum, storage and release of calcium; the dependence of muscle contraction and relaxation on the intracellular concentration of free calcium ions; troponin, the on-off switch of muscle contraction in striated muscle; diversity of fast and slow striated muscle; myosin-linked regulation of mollulscan muscle; the vertebrate heart, modulation of calcium control; vertebrate smooth muscle; principles of calcium signalling in muscle. The author has made many important contributions to this subject and has written an excellent monograph that will be the standard work for many years to come. Renal Physiology: Principles, Structure and Function---E. Koushanpour and W. Kriz. 2nd edn, 390 pp. 1986. Springer, Berlin. DM 124. The first edition of this text developed an integrated quantitative analysis of renal function and body fluid homeostasis using systems analysis. The second edition has brought the information up to date, with greater emphasis on the correlation between kidney structure and function. More emphasis is given to the role of capillary permeability and also the tubular processing of filtrate along the nephron. The chapters are: renal-body fluid regulating system; body fluids, normal volume and composition; body fluids, turn over rates and dynamics of fluid shift; overview of structure and functional organization of the kidney; form- ation of glomerular filtrate; regulation of renal blood flow and glomerular filtration rate; renal clearance; structural and biophysical basis of tubular transport; tubular process- ing of glomerular ultrafiltrate; mechanisms of electrolyte and water transport; tubular reabsorption and secretion; 637
Transcript of Advanced methods of protein microsequence analysis
Gen. Pharmac. Vol. 19, No. 4, pp. 637~538, 1988 0306-3623/88 $3.00 + 0.00 Printed in Great Britain. All rights reserved Copyright © 1988 Pergamon Press plc
BOOK REVIEWS
Manual of Pharmacologic Calculations with Computer Programs--R. J. Tallarida and R. B. Murray. 2nd edn, 297 pp. 1987. Springer, New York. $35.
If you do many calculations of the following type: dosage and concentrations; drug stock solutions; graded dose response; quantal dose response; relative potency; dissociation con- stants of agonists, partial agonists, perturbation methods; pA2 analysis; enzyme kinetics; drug decay; pharmaco- kinetics; statistical analysis of results (whole range of tests); then this book will be useful to you. It provides details of how to do these calculations on a micro and the software programs in Basic for each calculation so you can copy these programs and run them on your micro. It is also possible to purchase the programs on a disc (Version 4) and the original purchase price of this book is deducted from the disc price. The programs will run on MS-DOS computers (IBM and compatibles) and Tandy/Radio Shack TRS-80 models. If you already have an older version of the disc it can be credited to you and replaced by the new more complete version. This system will be very useful in all pharmacological teaching and research departments.
Cocaine, Clinical and Biobehaviourai Aspects---Edited by S. Fisher, A. Raskin and E. H. Uhlenhuth. 256 pp. 1987. Oxford University Press. $24.95.
Opiates like most substances are neutral in themselves but have good or bad effects depending on their application. Unfortunately the bad effects of cocaine are now dominant and this multi-authored volume describes the scientific basis for the abuses. The chapters are; the history of the use of cocaine; the pharmacological mechanisms of action; neur- onal bases for the hedonistic effects of cocaine and opiates; neural substrates for cocaine and opiate reinforcement; chronic cocaine administration, sensitization and kindling effects; cocaine abuse treatment research; clinical issues in cocaine abuse; coca and other psychoactive plants, magico- religious roles in primitive societies in the New World. The chapters are well written and provide a valuable scientific study of the present problems and research.
Advanced Methods of Protein Microseqnence Analysis-- Edited by B. Wittmann-Liebold, J. Salnikow and V. Erdmann. 423 pp. 1986. Berlin. DM 198,
Although recombinant DNA technique is very useful in determining protein structure, the direct sequencing of a protein can often provide a characterization of the poly- peptide structure. Protein fragmentation can be used to determine functional domains and active sites. It is estimated that in eukaryotic cells at least one third of all proteins may have the N or C terminus of the chain blocked. Sequence analysis also can provide information about the secondary and tertiary structure. Following a FEBS advanced course on microsequencing and an international symposium on sequencing, the editors asssembled the methods they are published in this multi-authored book. The chapters are; separation and AA analysis of proteins and peptides for microsequencing studies; manual and solid phase micro- sequencing methods; gas phase and radio-sequence analysis; phenylthiohydantoin identification, on-line detection, sequence control and data processing; analysis of cysteine
residues in proteins; methods of analysing protein conform- ation; strategies and specific examples of sequencing proteins and peptides: alternative sequencing techniques for elucid- ation of peptide and protein sequencing. Full practical details are given how to carry out each stage, how much material to use, what chemicals and equipment, the type of results obtained. The book will be very useful in all laboratories interested in sequencing.
Principles and Models of Biological Transport--Morton H. Friedman. 260 pp. 1986. Springer, Berlin. DM 165.
This test is aimed at the advanced undergraduate/graduate level and deals with equilibrium thermodynamics; free diffu- sion; facilitated diffusion; active transport; nonequilibrium thermodynamics; models of transport across cell mem- branes; single cells (erythrocytes, nerve, muscle); epithelial transport (small intestine, kidney, glomerular filtration, proximal tubule, loop of Henle, distal tubule, collecting tubule) gas transport. Its approach is rigorous with accent on the physical and mathematical explanations and gives the engineering attitude to biological transport.
Calcium in Muscle Activation: A Comparative Approach~ J. C. Ruegg. 300 pp. 1986. Springer, Berlin. DM 198.
Calcium plays a key role in controlling muscle contraction and relaxation and this single author work provides an excellent summary of the molecular basis of muscle move- ment. The chapters are: muscle excitation and contraction; the sarcoplasmic reticulum, storage and release of calcium; the dependence of muscle contraction and relaxation on the intracellular concentration of free calcium ions; troponin, the on-off switch of muscle contraction in striated muscle; diversity of fast and slow striated muscle; myosin-linked regulation of mollulscan muscle; the vertebrate heart, modulation of calcium control; vertebrate smooth muscle; principles of calcium signalling in muscle. The author has made many important contributions to this subject and has written an excellent monograph that will be the standard work for many years to come.
Renal Physiology: Principles, Structure and Function---E. Koushanpour and W. Kriz. 2nd edn, 390 pp. 1986. Springer, Berlin. DM 124.
The first edition of this text developed an integrated quantitative analysis of renal function and body fluid homeostasis using systems analysis. The second edition has brought the information up to date, with greater emphasis on the correlation between kidney structure and function. More emphasis is given to the role of capillary permeability and also the tubular processing of filtrate along the nephron.
The chapters are: renal-body fluid regulating system; body fluids, normal volume and composition; body fluids, turn over rates and dynamics of fluid shift; overview of structure and functional organization of the kidney; form- ation of glomerular filtrate; regulation of renal blood flow and glomerular filtration rate; renal clearance; structural and biophysical basis of tubular transport; tubular process- ing of glomerular ultrafiltrate; mechanisms of electrolyte and water transport; tubular reabsorption and secretion;
637
