Slide 1

ADULTMALE40 x 0,1 mm, yellowish white, cylinder, slender,Caudal part bends to ventral portion

Source: Color Atlas of Medicine and Parasitology. 1977W. Peters & H.M. Gillers

MORPHOLOGYWuchereria bancroftiWuchereria bancroftiADULTFEMALE(80-100) x (0,24-0,30) mm, soft, blunt nail, slight rounded head.

Sumber : Color Atlas of Medicine and Parasitology. 1977. W. Peters & H.M. Gillers

MORPHOLOGYMORPHOLOGY OF MICROFILARIAE

SheathSheathSlendertailCephalic spaceSource : Medical Parasitology in Plates. G. PiekarskiNuclei

Microfilaria Wuchereria bancrofti

Microfilaria Brugia malayi

Microfilaria Brugia timori

Source : Color Atlas of Medicine and Parasitology. 1977W. Peters & H.M. Gillers. Pewarnaan giemsaSource : Atlas of Medical Parasitology.Prayong Radomyos, dkk. Pewarnaan hematoxylinCephalic space(L:W=1:1)Tail(No nuclei)MICROFILARIAEWucheria bancroftiMORPHOLOGYB. malayi

W. bancrofti

Bloated tail (nucleus in it)Cephalic space(L:W=2:1)MICROFILARIAEBrugia malayiMORPHOLOGYLooking like microfilariae B. malayiSize 287-341 Sheathed not clear at coloration of Giemsa

Source : Atlas of Medical Parasitology. Prayong Radomyos, dkk.

MICROFILARIAEBrugia timoriMORPHOLOGY(dr. Djaenudin Natadisastra, SpParK)7

Source : Atlas of Medical Parasitology. Prayong Radomyos, dkk.

Stiff body broken, tip of tail rather bluntNuclei in the body, spread in the irregular groups, presence of (5-8) nuclei in the tail

Smaller nuclei at the distal than in B. malayiCephalic space: 3 : 1MICROFILARIAEBrugia timoriMORPHOLOGYFOUND TWO MAIN STAGE :TROPHOZOITE STAGERing formOld/Matured trophozoiteSCHIZONT STAGEYoung schizontOld/Matured schizontIN ERYTHOCYTIC CYCLESPlasmodium sp.GENERAL MORPHOLOGYRing Form with reddish Nucleus and bluish ProtoplasmChromatin dotsNucleusProtoplasmERYTHROCYTEMORPHOLOGYPlasmodium sp.TROPHOZOITE STAGENucleus and Protoplasm grow biggerERYTHROCYTENucleusProtoplasmMORPHOLOGYPlasmodium sp.TROPHOZOITE STAGENucleus starts fission processProtoplasm remains in sizeERYTHROCYTENucleusProtoplasmMORPHOLOGYPlasmodium sp.SCHIZONT STAGEComplete MerozoitesNote the Regularity, PigmentERYTHROCYTEMerozoiteMORPHOLOGYPlasmodium sp.SCHIZONT STAGEPLASMODIUM VIVAX

Ring FormErythrocyte enlarged, paleSchuffner dots

ERYTHROCYTERing FormERYHTROCYTETROPHOZOITE STAGEPLASMODIUM VIVAXProtoplasm grows (Amoeboid form) with vacuole

ERYTHROCYTEERYTHROCYTEAmoeboid FormTROPHOZOITE STAGEVacuoleFission of NucleusVacuole disappear

ERYTHROCYTEFission of NucleusPLASMODIUM VIVAX

SCHIZONT STAGEMerozoite (12-14)Pigment

MEROZOITePigmentPLASMODIUM VIVAX

SCHIZONT STAGERound/OvalSmall nucleus , compact, eccentricBluish protoplasmNUCLEUS

PLASMODIUM VIVAX

GAMOTOCYTE STAGE: Macro and MicrogametocyteRound/OvalBig nucleus, paleRed-blue protoplasmPigmentNUCLEUS

PLASMODIUM OVALE

Ring Form (thick) Erythrocyte Chromatin dotsTROPHOZOITE STAGEPLASMODIUM OVALE

Round, compact trophozoiteErythrocyte deformedSchuffner/James DotsTROPHOZOITE STAGEPlasmodium malariaeResemble P. vivaxNormal erythrocyte

TROPHOZOITE STAGEBand form TrophozoiteTrophozoite

TROPHOZOITE STAGEPlasmodium malariaeRossete-like Merozoit (6-12)

MEROZOITEPigmentSCHIZONT STAGEPlasmodium malariaeRound/OvalSmall nucleus, compactBluish protoplasm

GAMETOCYTE STAGE: Macro and MicrogametocytePlasmodium malariaeRound/OvalLarge nucleus, paleReddish protoplasm

TROPHOZOITE STAGE

Plasmodium falciparum

Fine ringMultiple infectionVacuole surrounded by plasmaMaurers dots

VacuolePlasmodium falciparumTROPHOZOITE STAGESchizont occupy 2/3 erythrocyte8-24 Merozoite

Plasmodium falciparumSCHIZONT STAGEBanana/crescent shapeSmall nucleus, compact, in the middlePigment around nucleusBluish protoplasmGAMETOCYTE STAGE: Macro and MicrogametocytePlasmodium falciparumSausage shapeLarge nucleus, pale, more eccentric positionPigment dispersedReddish protoplasm

UniformNo red zone around parasiteSmaller than lymphocyte nucleusPlasmodium falciparumTHICK BLOOD PREPARATION

Not uniformRed zone around parasiteBigger than lymphocyte nucleus

Not uniformNo red zone around parasiteSmaller than lymphocyte nucleus

PLASMODIUM VIVAXPlasmodium malariae