Additional File 2: Linear range of ERCC detection (A) microarray (B) RT-qPCR platforms: ERCC-42
description
Transcript of Additional File 2: Linear range of ERCC detection (A) microarray (B) RT-qPCR platforms: ERCC-42
Additional File 2: Linear range of ERCC detection (A) microarray (B) RT-qPCR platforms: ERCC-42
y = 1.0047x - 9.5772
R2 = 0.9675
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Log2[RNA copies/ng total RNA]
Lo
g2(
No
rma
lise
d s
ign
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y = -1.0205x + 42.617
R2 = 0.9988
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LogPCR Efficiency[RNA copies/ng total RNA]
Ct
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Additional File 2: Linear range of ERCC detection (A) microarray (B) RT-qPCR platforms: ERCC-81
y = 1.0389x - 10.155
R2 = 0.9671
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Log2[RNA copies/ng total RNA]
Lo
g2(N
orm
alis
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y = -0.9946x + 40.515
R2 = 0.9992
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LogPCR Efficiency[RNA copies/ng total RNA]
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Additional File 2: Linear range of ERCC detection (A) microarray (B) RT-qPCR platforms: ERCC-84
y = 0.9772x - 5.8678
R2 = 0.9639
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Log2[RNA copies/ng total RNA]
Lo
g2(N
orm
alis
ed s
ign
al)
y = -0.9693x + 41.49
R2 = 0.9986
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LogPCR Efficiency[RNA copies/ng total RNA]
Ct
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Additional File 2: Linear range of ERCC detection (A) microarray (B) RT-qPCR platforms: ERCC-95
y = 0.957x - 8.8564
R2 = 0.9712
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Log2[RNA copies/ng total RNA]
Lo
g2(N
orm
alis
ed s
ign
al)
y = -1.0022x + 44.939
R2 = 0.9992
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LogPCR Efficiency[RNA copies/ng total RNA]
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Additional File 2: Linear range of ERCC detection (A) microarray (B) RT-qPCR platforms: ERCC-99
y = 1.0095x - 6.6213
R2 = 0.9626
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Log2[RNA copies/ng total RNA]
Lo
g2(N
orm
alis
ed s
ign
al)
y = -1.0052x + 41.88
R2 = 0.9984
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LogPCR Efficiency[RNA copies/ng total RNA]
Ct
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Additional File 2: Linear range of ERCC detection (A) microarray (B) RT-qPCR platforms: ERCC-113
y = 1.0248x - 6.6933
R2 = 0.9993
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Log2[RNA copies/ng total RNA]
Lo
g2(N
orm
alis
ed s
ign
al)
y = -1.02x + 42.406
R2 = 0.9974
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LogPCR Efficiency[RNA copies/ng total RNA]
Ct
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Additional File 2: Linear range of ERCC detection (A) microarray (B) RT-qPCR platforms: ERCC-171
y = 1.0696x - 10.718
R2 = 0.9993
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Log2[RNA copies/ng total RNA]
Lo
g2(N
orm
alis
ed s
ign
al)
y = -0.9714x + 44.299
R2 = 0.9981
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LogPCR Efficiency[RNA copies/ng total RNA]
Ct
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