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Analytical Chemistry: Volume 81, Issue 4 (ACS Publications)

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Table of Contents

Larger Cover February 15, 2009Volume 81, Issue 4Pages 1297-1722

In this issue:

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Container of nuclear material excavated from a nuclear waste burial site. The article is by Jon Schwantes, Matthew Douglas, Steven Bonde, James Briggs, Orville Farmer, Lawrence Greenwood, Elwood Lepel, Christopher Orton, John Wacker, and Andrzej Luksic. View the article.

indicates the article is freely available through sponsorship by the author or related funding agency. More about this program.

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Accelerated Articles

Nuclear Archeology in a Bottle: Evidence of Pre-Trinity U.S. Weapons Activities from a Waste Burial Site

Jon M. Schwantes*, Matthew Douglas, Steven E. Bonde, James D. Briggs, Orville T. Farmer, Lawrence R. Greenwood, Elwood A. Lepel, Christopher R. Orton, John F. Wacker and Andrzej T. Luksic pp 1297–1306 Publication Date (Web): January 16, 2009 (Accelerated Article)DOI: 10.1021/ac802286aAbstract

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Articles

Relationship between Sample Loading Amount and Peptide Identification and Its Effects on Quantitative Proteomics


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Kehui Liu†, Jiyang Zhang†‡, Jinglan Wang†, Liyan Zhao†, Xu Peng†, Wei Jia†, Wantao Ying†, Yunping Zhu†, Hongwei Xie‡, Fuchu He† and Xiaohong Qian*† pp 1307–1314 Publication Date (Web): January 15, 2009 (Article)DOI: 10.1021/ac801466kAbstract | Supporting Info

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Data-Driven Optimization of Metabolomics Methods Using Rat Liver Samples

Gauri S. Parab†, Raghuraj Rao§, Samavedham Lakshminarayanan§, Yap Von Bing , Shabbir M. Moochhala‡ and Sanjay Swarup*† pp 1315–1323 Publication Date (Web): January 13, 2009 (Article)DOI: 10.1021/ac801645tAbstract

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Label-Free Separation of Human Embryonic Stem Cells and Their Cardiac Derivatives Using Raman Spectroscopy

James W. Chan*†‡, Deborah K. Lieu‡§ , Thomas Huser‡ and Ronald A. Li*§ pp 1324–1331



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Double-Tagging Polymerase Chain Reaction with a Thiolated Primer and Electrochemical Genosensing based on Gold Nanocomposite Sensor for Food Safety

Paulo R. Brasil de Oliveira Marques†, Anabel Lermo‡, Susana Campoy§, Hideko Yamanaka†, Jordi Barb §, Salvador Alegret‡ and M. Isabel Pividori*‡ pp 1332–1339 Publication Date (Web): January 26, 2009 (Article)DOI: 10.1021/ac801736bAbstract | Supporting Info

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Carbon Nanoparticle-Enhanced Immunoelectrochemical Detection for Protein Tumor Marker with Cadmium Sulfide Biotracers

Ja-an Annie Ho*†, Yeh-Chun Lin†, Li-Sheng Wang†, Kuo-Chu Hwang‡ and Pi-Tai Chou§ pp 1340–1346



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Noncovalent Protein Tetramers and Pentamers with “n” Charges Yield Monomers with n/4 and n/5 Charges

Richard L. Beardsley†, Christopher M. Jones, Asiri S. Galhena‡ and Vicki H. Wysocki*pp 1347–1356 Publication Date (Web): January 13, 2009 (Article)DOI: 10.1021/ac801883kAbstract | Supporting Info

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Development of a Robust and Repeatable UPLC−MS Method for the Long-Term Metabolomic Study of Human Serum

Eva Zelena†, Warwick B. Dunn*‡, David Broadhurst†, Sue Francis-McIntyre†, Kathleen M. Carroll‡, Paul Begley†, Steve O’Hagan†, Joshua D. Knowles§, Antony Halsall†, , Ian D. Wilson and Douglas B. Kell†‡ pp 1357–1364 Publication Date (Web): January 26, 2009 (Article)DOI: 10.1021/ac8019366



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Integration of Low-Power Microfluidic Pumps with Biosensors within a Laboratory-on-a-Chip Device

Gerald Blanco-Gomez†, Andrew Glidle†, Leonard M. Flendrig‡ and Jon M. Cooper*† pp 1365–1370 Publication Date (Web): January 14, 2009 (Article)DOI: 10.1021/ac802006dAbstract

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Polymerase Chain Reaction-Capillary Electrophoresis Genetic Analysis Microdevice with In-Line Affinity Capture Sample Injection

Numrin Thaitrong†, Nicholas M. Toriello‡, Nadia Del Bueno‡ and Richard A. Mathies*†‡ pp 1371–1377 Publication Date (Web): January 13, 2009 (Article)DOI: 10.1021/ac802057fAbstract | Supporting Info



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Sensitive and Colorimetric Detection of the Structural Evolution of Superoxide Dismutase with Gold Nanoparticles

Surin Hong†, Inhee Choi†, Suseung Lee†, Young In Yang†, Taewook Kang‡ and Jongheop Yi*† pp 1378–1382 Publication Date (Web): January 26, 2009 (Article)DOI: 10.1021/ac802099cAbstract

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Real-Time Monitoring of the Activity and Kinetics of T4 Polynucleotide Kinase by a Singly Labeled DNA-Hairpin Smart Probe Coupled with λ Exonuclease Cleavage

Chen Song and Meiping Zhao*pp 1383–1388 Publication Date (Web): January 26, 2009 (Article)DOI: 10.1021/ac802107wAbstract | Supporting Info

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Scattering Orthogonalization of Near-Infrared Spectra for Analysis of Pharmaceutical Tablets

Zhenqi Shi and Carl A. Anderson* pp 1389–1396 Publication Date (Web): January 22, 2009 (Article)DOI: 10.1021/ac802105vAbstract

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Aluminum Nanoparticles as Substrates for Metal-Enhanced Fluorescence in the Ultraviolet for the Label-Free Detection of Biomolecules

Mustafa H. Chowdhury†, Krishanu Ray†, Stephen K. Gray‡, James Pond§ and Joseph R. Lakowicz*† pp 1397–1403 Publication Date (Web): January 21, 2009 (Article)DOI: 10.1021/ac802118sAbstract | Supporting Info

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Method for the Determination of Pd-Catalyst Residues in Active Pharmaceutical Ingredients by Means of High-Energy Polarized-Beam Energy Dispersive X-Ray Fluorescence

E. Margu *†, K. Van Meel‡, R. Van Grieken‡, A. Buend a§, C. Font s , M. Hidalgo and I. Queralt† pp 1404–1410 Publication Date (Web): January 22, 2009 (Article)DOI: 10.1021/ac8021373Abstract

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In Vivo Profiling Endogenous Interactions with Knock-Out in Mammalian Cells

Ling Xie†, Linhong Jing‡, Yanbao Yu†§, Kazuhiro Nakamura , Carol E. Parker‡, Gary L. Johnson and Xian Chen*†‡

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Nanosensors Based on Viologen Functionalized Silver Nanoparticles: Few Molecules Surface-Enhanced Raman Spectroscopy Detection of Polycyclic Aromatic Hydrocarbons in Interparticle Hot Spots

Luca Guerrini*, Jos V. Garcia-Ramos, Concepci n Domingo and Santiago Sanchez-Cortespp 1418–1425 Publication Date (Web): January 21, 2009 (Article)DOI: 10.1021/ac8021746Abstract | Supporting Info

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Quantitative Fluorescence Correction Incorporating F rster Resonance Energy Transfer and Its Use for Measurement of Hybridization Efficiency on Microarrays

Jiang Zhu†‡, Cheng Deng†‡, Guoliang Huang*†‡§, Shukuan Xu†‡, Keith Mitchelson†‡ and Jing Cheng*†‡§

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Top-Down Protein Identification/Characterization of a Priori Unknown Proteins via Ion Trap Collision-Induced Dissociation and Ion/Ion Reactions in a Quadrupole/Time-of-Flight Tandem Mass Spectrometer

Jian Liu, Teng-Yi Huang and Scott A. McLuckey*pp 1433–1441 Publication Date (Web): January 26, 2009 (Article)DOI: 10.1021/ac802204jAbstract

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Use of Raman Microscopy and Multivariate Data Analysis to Observe the Biomimetic Growth of Carbonated Hydroxyapatite on Bioactive Glass

Regina K. H. Seah†, Marc Garland‡, Joachim S. C. Loo*† and Effendi Widjaja*‡ pp 1442–1449 Publication Date (Web): January 26, 2009 (Article)DOI: 10.1021/ac802234tAbstract

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Determination of the Composition of Fatty Acid Mixtures Using GC × FI-MS: A Comprehensive Two-Dimensional Separation Approach

Leila Hejazi†‡, Diako Ebrahimi*†, Michael Guilhaus‡ and D. Brynn Hibbert*† pp 1450–1458 Publication Date (Web): January 15, 2009 (Article)DOI: 10.1021/ac802277cAbstract

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Measuring the Length Distribution of Self-Assembled Lipid Nanotubes by Orientation Control with a High-Frequency Alternating Current Electric Field in Aqueous Solutions

Ken Hirano*†, Masaru Aoyagi‡, Tomomi Ishido†, Toshihiko Ooie†, Hiroshi Frusawa§, Masumi Asakawa‡, Toshimi Shimizu‡ and Mitsuru Ishikawa† pp 1459–1464 Publication Date (Web): January 26, 2009 (Article)DOI: 10.1021/ac8022795Abstract | Supporting Info

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Design of Fluorescent Assays for Cyanide and Hydrogen Peroxide Based on the Inner Filter Effect of Metal Nanoparticles

Li Shang and Shaojun Dong*pp 1465–1470 Publication Date (Web): January 13, 2009 (Article)DOI: 10.1021/ac802281xAbstract | Supporting Info

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Partition Coefficient Measurements in Picoliter Drops Using a Segmented Flow Microfluidic Device

Nathan A. Marine†, Steven A. Klein† and Jonathan D. Posner*†‡ pp 1471–1476 Publication Date (Web): January 14, 2009 (Article)DOI: 10.1021/ac801673wAbstract | Supporting Info

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Serial-to-Parallel Interfaces for Efficient Sample Transfer on Microfluidic Devices

Zexi Zhuang and Stephen C. Jacobson*pp 1477–1481 Publication Date (Web): January 16, 2009 (Article)DOI: 10.1021/ac801774pAbstract | Supporting Info

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High-Resolution Ion Cyclotron Mobility Spectrometry

Samuel I. Merenbloom, Rebecca S. Glaskin, Zachary B. Henson and David E. Clemmer*pp 1482–1487 Publication Date (Web): January 14, 2009 (Article)DOI: 10.1021/ac801880aAbstract

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Low Thermal Mass Liquid Chromatography

Binghe Gu†, Hernan Cortes*†, Jim Luong‡, Matthias Pursch§, Patric Eckerle§ and Robert


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Mustacichpp 1488–1495 Publication Date (Web): January 13, 2009 (Article)DOI: 10.1021/ac802022zAbstract

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Quantitative Assessment of Hepatic Fat of Intact Liver Tissues with Coherent Anti-Stokes Raman Scattering Microscopy

Yao-Ming Wu†, Hung-Che Chen‡, Wei-Tien Chang†, Jhen-Wei Jhan‡, Hung-Lung Lin‡ and Ian Liau*‡ pp 1496–1504 Publication Date (Web): January 16, 2009 (Article)DOI: 10.1021/ac8026838Abstract

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Nuclear Magnetic Resonance and Electron Paramagnetic Resonance as Analytical Tools To Investigate Structural Features of Archaeological Leathers

Michel Bardet*†, Guillaume Gerbaud†, Laurent Le Pape†‡, Sabine Hediger† , Qu c-Kh i Tr n§



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and N bia Boumlil§ pp 1505–1511 Publication Date (Web): January 21, 2009 (Article)DOI: 10.1021/ac802052aAbstract

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Molecular Characterization of Biomass Burning Aerosols Using High-Resolution Mass Spectrometry

Jeffrey S. Smith†‡, Alexander Laskin§ and Julia Laskin*† pp 1512–1521 Publication Date (Web): December 30, 2008 (Article)DOI: 10.1021/ac8020664Abstract

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Probe for Spin- and Valence-Selective X-ray Absorption Fine Structure Spectroscopy: EuLγ4 Emission

Hisashi Hayashi*†, Naomi Kawamura‡, Masaichiro Mizumaki‡ and Toshiro Takabatake§ pp 1522–1528 Publication Date (Web): January 26, 2009 (Article)





















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Detection of Intact Ricin in Crude and Purified Extracts from Castor Beans Using Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

Craig S. Brinkworth*, Eloise J. Pigott and David J. Bournepp 1529–1535 Publication Date (Web): January 21, 2009 (Article)DOI: 10.1021/ac802240fAbstract | Supporting Info

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Silicone Membrane Equilibrator: Measuring Chemical Activity of Nonpolar Chemicals with Poly(dimethylsiloxane) Microtubes Immersed Directly in Tissue and Lipids

Philipp Mayer*†, Lars Tor ng†§, Nadia Glæsner† and Jan Åke J nsson‡ pp 1536–1542 Publication Date (Web): January 15, 2009 (Article)DOI: 10.1021/ac802261z
























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Electrochemical Determination of Thioredoxin Redox States

Vlastimil Dor k and Emil Pale ek*pp 1543–1548 Publication Date (Web): January 26, 2009 (Article)DOI: 10.1021/ac802274pAbstract | Supporting Info

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High-Performance Immunolatex Possessing a Mixed-PEG/Antibody Coimmobilized Surface: Highly Sensitive Ferritin Immunodiagnostics

Xiaofei Yuan†‡§, Keitaro Yoshimoto†‡§ and Yukio Nagasaki*†‡§ pp 1549–1556 Publication Date (Web): January 22, 2009 (Article)DOI: 10.1021/ac802282cAbstract | Supporting Info

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Dried Blood Spots as a Sample Collection Technique for the Determination of Pharmacokinetics in Clinical Studies: Considerations for the Validation of a Quantitative Bioanalytical Method

Neil Spooner*, Rakesh Lad and Matt Barfield pp 1557–1563 Publication Date (Web): January 20, 2009 (Article)DOI: 10.1021/ac8022839Abstract

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Pyrroloquinoline Quinone-Doped Polymeric Nanospheres as Sensitive Tracer for Binding Assays

Dongxuan Shen and Mark E. Meyerhoff*pp 1564–1569 Publication Date (Web): January 22, 2009 (Article)DOI: 10.1021/ac8023153Abstract | Supporting Info

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Multiplexed Four-Channel Rectilinear Ion Trap Mass Spectrometer

Sameer Kothari†, Qingyu Song†, Yu Xia†, Miriam Fico†, Dennis Taylor‡, Jonathan W. Amy†‡, George Stafford‡ and R. Graham Cooks*† pp 1570–1579 Publication Date (Web): January 27, 2009 (Article)DOI: 10.1021/ac8023284Abstract | Supporting Info

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Rapid Profiling of Peptide Stability in Proteolytic Environments

Hans H. Gorris†‡ , Steffen Bade†, Niels R ckendorf†, Eike Albers‡ , M. Alexander Schmidt‡, Milan Fr nek§ and Andreas Frey*† pp 1580–1586 Publication Date (Web): January 21, 2009 (Article)DOI: 10.1021/ac802324fAbstract | Supporting Info

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Digitally-Multiplexed Nanoelectrospray Ionization Atmospheric Pressure Drift Tube Ion Mobility Spectrometry

Mark Kwasnik, Joe Caramore† and Facundo M. Fern ndez* pp 1587–1594 Publication Date (Web): January 9, 2009 (Article)DOI: 10.1021/ac802383kAbstract

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Real-Time Monitoring of Hazardous Air Pollutants

Gregory J. Francis†‡, Vaughan S. Langford†, Daniel B. Milligan† and Murray J. McEwan*†‡ pp 1595–1599 Publication Date (Web): January 21, 2009 (Article)DOI: 10.1021/ac802510hAbstract

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Enzyme-Functionalized Silica Nanoparticles as Sensitive





















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Yafeng Wu, Chengliang Chen and Songqin Liu*pp 1600–1607 Publication Date (Web): January 13, 2009 (Article)DOI: 10.1021/ac802345zAbstract | Supporting Info

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Reagentless, Electrochemical Approach for the Specific Detection of Double- and Single-Stranded DNA Binding Proteins

Francesco Ricci†‡, Andrew J. Bonham§, Aaron C. Mason , Norbert O. Reich‡§ and Kevin W. Plaxco*‡§ pp 1608–1614 Publication Date (Web): January 21, 2009 (Article)DOI: 10.1021/ac802365xAbstract

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Surface Molecular Imprinting on Mn-Doped ZnS Quantum Dots for Room-Temperature Phosphorescence Optosensing of Pentachlorophenol in Water




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He-Fang Wang, Yu He, Tian-Rong Ji and Xiu-Ping Yan*pp 1615–1621 Publication Date (Web): January 26, 2009 (Article)DOI: 10.1021/ac802375aAbstract

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Design and Evaluation of a Passive Alcove-Based Microfluidic Mixer

Tsuyoshi Egawa, Jorge L. Durand, Eric Y. Hayden, Denis L. Rousseau* and Syun-Ru Yeh*pp 1622–1627 Publication Date (Web): January 13, 2009 (Article)DOI: 10.1021/ac802410gAbstract

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Evaluation of a Microfluidic Device for the Electrochemical Determination of Halide Content in Ionic Liquids

R. Ge†, R. W. K. Allen‡, L. Aldous†, M. R. Bown‡, Nicola Doy§, C. Hardacre*†, J. M. MacInnes*‡, G. McHale§ and M. I. Newton§ pp 1628–1637 Publication Date (Web): January 22, 2009 (Article)









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DOI: 10.1021/ac802406kAbstract

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Detection of Glucose Based on Direct Electron Transfer Reaction of Glucose Oxidase Immobilized on Highly Ordered Polyaniline Nanotubes

Ziyi Wang, Shuna Liu, Ping Wu and Chenxin Cai*pp 1638–1645 Publication Date (Web): January 26, 2009 (Article)DOI: 10.1021/ac802421hAbstract

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Method for Determining Double-Bond Positions in Mixtures of Linear Olefins

Jeffrey C. Gee* and Daniel S. Prampinpp 1646–1651 Publication Date (Web): January 22, 2009 (Article)DOI: 10.1021/ac802449xAbstract























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Technique for Microfabrication of Polymeric-Based Microchips from an SU-8 Master with Temperature-Assisted Vaporized Organic Solvent Bonding

Myra T. Koesdjojo, Corey R. Koch and Vincent T. Remcho*pp 1652–1659 Publication Date (Web): January 23, 2009 (Article)DOI: 10.1021/ac802450uAbstract

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Disposable Nucleic Acid Biosensors Based on Gold Nanoparticle Probes and Lateral Flow Strip

Xun Mao†, Yunqing Ma‡, Aiguo Zhang§, Lurong Zhang , Lingwen Zeng* and Guodong Liu*†

pp 1660–1668 Publication Date (Web): January 21, 2009 (Article)DOI: 10.1021/ac8024653Abstract | Supporting Info

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Analysis of Biological Fluids by Direct Combination of Solid Phase Extraction and Desorption Electrospray Ionization Mass Spectrometry

J lia D nes†, M ria Katona†, d m Hossz †, No mi Czuczy† and Zolt n Tak ts*†‡ pp 1669–1675 Publication Date (Web): January 22, 2009 (Article)DOI: 10.1021/ac8024812Abstract | Supporting Info

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Application of HPLC and MALDI-TOF MS for Studying As-Synthesized Ligand-Protected Gold Nanoclusters Products

Yan Zhang†§, Shaomin Shuang*†, Chuan Dong†, Chung Keung Lo‡, Man Chin Paau‡ and Martin M. F. Choi*‡

pp 1676–1685 Publication Date (Web): January 26, 2009 (Article)DOI: 10.1021/ac8026349Abstract | Supporting Info

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Direct Identification and Quantification of Aspartyl Succinimide in an IgG2 mAb by RapiGest Assisted Digestion

Holly Z. Huang*†, Andrew Nichols‡ and Dingjiang Liu†

pp 1686–1692 Publication Date (Web): January 15, 2009 (Article)DOI: 10.1021/ac802708sAbstract | Supporting Info

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Technical Notes

Peptide Quantification Using 8-Plex Isobaric Tags and Electron Transfer Dissociation Tandem Mass Spectrometry

Doug Phanstiel†, Richard Unwin‡, Graeme C. McAlister† and Joshua J. Coon*†§ pp 1693–1698 Publication Date (Web): January 20, 2009 (Technical Note)DOI: 10.1021/ac8019202Abstract

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Indirect Modulation of Nonmagnetic Probes for Force Modulation Atomic Force Microscopy

Jie-Ren Li and Jayne C. Garno*pp 1699–1706 Publication Date (Web): January 21, 2009 (Technical Note)DOI: 10.1021/ac802143gAbstract

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Visual Sandwich Immunoassay System on the Basis of Plasmon Resonance Scattering Signals of Silver Nanoparticles

Jian Ling, Yuan Fang Li and Cheng Zhi Huang*pp 1707–1714 Publication Date (Web): January 27, 2009 (Technical Note)DOI: 10.1021/ac802152bAbstract | Supporting Info

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Development of a Multilane Channel System for Nongel-Based














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Two-Dimensional Protein Separations Using Isoelectric Focusing and Asymmetrical Flow Field-Flow Fractionation

Ki Hun Kim and Myeong Hee Moon*pp 1715–1721 Publication Date (Web): January 22, 2009 (Technical Note)DOI: 10.1021/ac802357sAbstract

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Additions and Corrections

Functional Nanoparticle-Based Proteomic Strategies for Characterization of Pathogenic Bacteria

Wei-Jen Chen, Pei-Jane Tsai and Yu-Chie Chenp 1722 Publication Date (Web): January 22, 2009 (Addition/Correction)DOI: 10.1021/ac802751dAbstract | Supporting Info

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