Achieving rice biopharma readiness in the philippines: · PDF fileachieving rice biopharma...
Transcript of Achieving rice biopharma readiness in the philippines: · PDF fileachieving rice biopharma...
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ACHIEVING RICE BIOPHARMA READINESS
IN THE PHILIPPINES: CHALLENGES AND
OPPORTUNITIES IN BIOTECH
RESEARCH FOR DEVELOPMENT
Amor A. San Juan, MSc., Ph.Demail: [email protected]
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RICE GRAIN: FROM STAPLE STARCH INTO
BIOPHARMACEUTICAL USE
Traditionally: staple food source Revolutionary: transgenic rice into drug
Image: Ricepedia
Rice flour
Rice bran
E
d
i
b
l
e
Rice winePuffed rice
Rice straw
Rice hull
Rice paper
n
o
n
–
e
d
i
b
l
Tokyo Future Style, Inc.
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BIOPHARMA: CROPS THAT PRODUCE DRUGS
TRADITIONAL PHARMA BIOPHARMA
Source – chemical synthesis
Size – small-molecule drug
Composition – organic molecule
Advantage – oral intake
Source – biological with genetic modification
Size – large, complex molecule drug
Composition – complex protein
Advantages – target-specific; increased
potency, fewer side effects
Transition from traditional pharmaceutical into biopharmaceutical
Patent Gap is widening
Jones, N., Dean, A., Pharm. Pat. Anal., 2012, 1: 225-7.
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DEVELOPMENT OF RICE-BASED EDIBLE DRUGS
PhilRice clipart
2016 – rice-based oral
cholera vaccine
2012 – rice-derived recombinant
lactoferrin
2013 – fibroblast growth factor
expressed in rice
2011 – human serum albumin
expressed in rice
2010 – recombinant transferrin
expressed in rice
2012 – human alpha-antitrypsin
expressed in riceNOVEL Rice-based THERAPEUTICS
R &
D
Ph
ase
1
Ph
ase
2
Ph
ase
3
Mar
kete
d
Product Indication YearMucoRice Oral cholera vaccine 2016
VEN100 Antibiotic-associated diarrhea 2012
rFGF-2 Tissue repair 2013
rHSA Hemorrhagic shock, burn injuries, cirrhotic ascites 2011
rTr Safe and low-cost alternative to blood-derived transferrin 2010
AAT Safe and low-cost alternative to alpha-antitrypsin 2012
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MucoRice: ANTIBODY-PRODUCING RICE
RECENTLY IN CLINICAL TRIAL
Advantages of rice-derived drug:
- Cost-effective
- Scalability (bioreactors)
- Resistance to stomach enzymatic
digestion
- Avoid contamination from human
pathogens
“A bowl of rice a day, keeps the needle away. “
Tokuhara., D. et al. J. Clin. Invest., 2013, 1: 225-7.
Process of introducing the rotavirus
antibody into the rice genome
Nochi., T. et al. Proc. Nat Acad Sci ., 2007, 104:10986-91.
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MucoRice: ANTIBODY-PRODUCING RICE
RECENTLY IN CLINICAL TRIAL
Advantages of rice-derived drug:
- Cost-effective
- Scalability (bioreactors)
- Resistance to stomach enzymatic
digestion
- Avoid contamination from human
pathogens
“A bowl of rice a day, keeps the needle away. “
Tokuhara., D. et al. J. Clin. Invest., 2013, 1: 225-7.
Process of introducing the rotavirus
antibody into the rice genome
Nochi., T. et al. Proc. Nat Acad Sci ., 2007, 104:10986-91.
J a p a n
Philippines
Philippines few list of plant-based drugs:
1. ASCOF Lagundi (cough)
2. RE-LEAF Sambong (diuretic)
3. Ampalaya Plus (blood-sugar))
Rice-based drug,
can we do here in
the Philippines ?
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PHILIPPINE RICE BIOPHARMA: VISION &
STRATEGIES TO MEET CHALLENGES
VISION STRATEGIES CHALLENGES
Innovation born
from diversityRethink: funding bottleneck
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CONSENSUS EFFORT IN BIOTECH
RESEARCH FOR DEVELOPMENT
Policy-makers
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SWOT ANALYSIS
PHILIPPINES RICE BIOPHARMACEUTICAL
Key issue
Existing infrastracture A strong strategy Build counterpart research team
(PhilRice) must leverage with IRRI, and international lead
collaboration to experts
exploit talent &
resources
Increasing economic A strong strategy Grow investment in R&D,
stability & safety in business must influence and become competitive
environment SC to favor with ASEANs S&T dev’t
biotech research
& commercialization
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SWOT ANALYSIS
PHILIPPINES RICE BIOPHARMACEUTICAL
Key issue
Bureaucracy in allocation A strong strategy Remain less competitive in
of research funds must have biotech compared to
transparency & neighboring ASEAN countries
efficiency in gov’t
research funding
Lack of support A strong strategy Brain-drain, talented filipino
for a scientific culture must foster young scientists remained abroad
generation into resulting to shortage of
science manpower technical
competence
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RICE BIOPHARMACEUTICAL RESEARCH
(RBR) IN THE PHILIPPINES TOWARDS
CUTTING-EDGE THERAPEUTICS
The Economist Intelligence Unit Limited 2015
While research
innovation greatly
impacts rice
security, would RBR
be a threat ?
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ACHIEVING SUCCESSFUL RBR PROJECT
IN THE PHILIPPINES
Science-people is the
key to knowledge
creation and success
in R&D
R&D Talent
Identify areas of cost,
risk and bottlenecks
If necessary, outsource
other experimental
protocols
R&D Process
Translates innovative
drug into market, by
partnering with
pharmaceutical company
Drives economic growth
R&D Market Technology
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ACKNOWLEDGEMENT
PhilRice
Evora university, Portugal
A-STAR, Singapore
Photo: Wikipedia
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Presentation Flow
• Introduction• Rice and its By-products
• Anthocyanins
• Encapsulation Technology
• Objectives of the Study
• Materials and Methods used
• Findings of the Study
• Conclusions
• For Future Works
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18.15 million metric
tons of palay in 2015
(PSA, 2016)
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Rice By-products
Hull
Stalks
Bran
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Fibers
Proteins
Minerals
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Pigmented Rice Varieties
Anthocyanins
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Flavonoid
Anthocyanins
reactive and unstable
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+ →Bioactive
Compound Encapsulating
MaterialNano/Microcapsules
1 μm < Micro < 1000 μm
1 nm < Nano < 1000 nm
Encapsulation Technology
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Chitosan
Alginate
Chitosan and Alginate
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To evaluate its encapsulation efficiency and
antioxidant activity
To screen black rice bran with the highest
anthocyanin content
To encapsulate anthocyanin using chitosan-
alginate
To characterize anthocyanin-loaded
capsules
Objectives of the Study
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Materials & Methods
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Rice Processing
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Anthocyanin Screening of Rice Bran
Samples
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Extraction of Anthocyanin
Defatting acidified ethanol extraction
Freeze drying
Freeze-dried
extract
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Encapsulation
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Encapsulation Treatments
Treatments Composition
T0 Blank chitosan-alginate
T1 Chitosan-alginate + 10 mg CAE
T2 Chitosan-alginate + 20 mg CAE
T3 Chitosan-alginate + 30 mg CAE
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Characterization and Evaluation of the
Anthocyanin-loaded Capsules
Characterization
• Chemical Properties
• Surface Morphology
• Particle Size
Evaluation
• Encapsulation Efficiency
• Antioxidant Activity
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Results
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Rice Samples
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→
←
←
Screening of Rice Bran Samples
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0.55
4.4
10.72
5.26
7.08
7.09
3.46
5.27
36.11
3.73
19.58
0 5 10 15 20 25 30 35 40
NSIC Rc160
Galo 1
Galo Malagkit
Inatipan
Ittum
Kotinaw
Labitaris
Masbate
Ominio
Palanqui
Pirurutong
Anthocyanin content (mg/g)
Ric
e B
ran
Sam
ple
sAnthocyanin Content of Rice Samples
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Chemical Properties
Fourier Transfrom Infrared Spectroscopy
Horizon MB3000
Characterization
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FTIR spectra of Chitosan-alginate
C-O stretch
Amide C=0 stretch
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FTIR spectra of loaded capsules
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Surface Morphology
Scanning Electron Microscopy
SEM (Hitachi S3400N)
Ion Sputter (Hitachi E1010)
Characterization
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SEM
Images
T0
T2 T3
T1
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Particle size (Zeta Sizer)
Treatment Mean Particle Size (nm) Polydispersive Index
T0 358.5±73.2a 0.57±0.02b
T1 572.3±125.8b 0.69±0.09bc
T2 635.9±88.4b 0.76±0.07c
T3 467.8±17.0ab 0.31±0.08a
Characterization
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Encapsulation Efficiency
UV-Vis Spectrophotometric method
Treatments Encapsulation Efficiency (%)
T1 56.87±0.08b
T2 56.34±0.60b
T3 68.92±1.29a
Evaluation
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DPPH radical scavenging activity
8.43b7.06b
38.32a
0
5
10
15
20
25
30
35
40
45
50
T1 T2 T3
% S
cave
ngin
g A
ctiv
ity
Treatments
Evaluation
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Ominio bran was found to contain
the highest amount of anthocyanin
Chitosan-alginate encapsulated the anthocyanin
extracted from black rice bran with a final product
of black to purple powder.
Conclusions
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Chemical properties well-incorporated
anthocyanin in the chitosan-alginate
framework.
Surface morphology presence of free
particles and clumping on the surface
Particle size nanocapsules
Conclusions
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T3 (30 mg CAE) highest encapsulation
efficiency and antioxidant scavenging
activity
Conclusions
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Increase the amount of CAE to encapsulate
to determine maximum loading capacity
Determine the release profile of the
capsules using in-vitro digestion and animal
clinical trials to determine its effectiveness
in-vivo
For Future Works
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Thank you!
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Antioxidant Capacities of Raw and Cooked Forms of some
Philippine Vegetables
Rosaly V. Manaois
John Edward I. Zapater
Amelia V. Morales
Rice Chemistry and Food Science Division
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Antioxidants• Can be a vitamin,
Ascorbic Acid Selenium Phenolics
a mineral or a phytochemical
• Able to prevent, delay or neutralize cellular damage.
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Phenolic Compounds• Phytochemicals with hydroxyl constituents attached
to a benzene ring.
• One of the most common compounds found in plants.
• Protect plants from environmental stresses.
Hydroxycinnamic acid Heat Cold Bacteria and Viruses
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The Current Picture• Antioxidants in cultivated fruits and vegetables in
many countries have been profiled.
Geography Climate Varieties Farming Practices
• However, they have different:
• Limited data on antioxidant capacities of raw and cooked locally cultivated and consumed vegetables
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Objectives• Measure the total phenolic content (TPC) of 21 vegetables
commonly grown in rice-based farms.
• Determine the antioxidant capacities of the vegetables using two commonly used antioxidant assays:
o 2,2-diphenylpicrylhydrazyl (DPPH)
o 2,2-azinobis(2,3-benzothiazoline-6-sulfonic acid) (ABTS)
• Compare TPC and antioxidant capacities of raw and boiled vegetables.
• Correlate TPC with the DPPH and ABTS radical scavenging activities.
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MethodologyLeafy and Salad Vegetables
Jute / Saluyot(Corchorus olitorius)
Mustard / Mustasa(Brassica juncea)
Chinese Cabbage / Pechay(Brassica rapa)
Spinach / Alugbati(Spinacia oleracea)
Water Spinach / Kangkong(Ipomoea aquatica)
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Botanical Fruits
Bitter Gourd / Ampalaya(Momordica charantia)
Bottle Gourd / Upo(Lagenaria siceraria)
Eggplant / Talong (Solanum melongena)
Lady’s fingers / Okra(Abelmoschus esculentus)
Squash / Kalabasa(Cucurbita maxima)
Green Pepper / Siling Panigang(Capsicum annuum)
Chili / Siling Labuyo(Capsicum frutescens)
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Rhizome
Ginger / Luya(Zingiber officinale)
Tubers
Sweet Potato / Kamote(Ipomoea batatas)
Taro / Gabi(Colocasia esculenta)
Edible Stalk
Cereal
Corn / Mais(Zea mays)
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Edible Flower
Squash Flower / Bulaklak ng Kalabasa(Cucurbita maxima)
Edible Mushroom
Oyster Mushroom / Kabute(Pleurotus ostreatus)
String Beans / Sitaw na Haba(Vigna unguiculata subsp. Sesquipedalis)
Cowpea / Sitaw na Turo(Vigna unguiculata)
Legumes
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Methodology
Vegetable
Remove non-edible parts
Raw Boiled
Oven-dry (40oC, ≥12 hr)
Defat with 1:1 hexane:dichloromethane
Total
Phenolic
Content
DPPH
Radical
Scavenging
Activity
ABTS
Radical
Scavenging
Activity
Extract with 85% methanol
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Results
Raw: 1.13-31.78 mg GAE/g
0
5
10
15
20
25
30
35B
itte
r G
ou
rd
Bo
ttle
Go
urd
Ch
ili P
ep
per
Ch
ine
se C
abb
age
Co
rn
Co
wp
ea
Eggp
lan
t
Gin
ger
Gre
en
Pe
pp
er
Jute
Mu
star
d
Okr
a
Squ
ash
Flo
we
r
Squ
ash
Fru
it
Spin
ach
Stri
ng
Be
ans
Swe
et
Po
tato
Taro
Sta
lk
Taro
Tu
be
r
Wat
er
Spin
ach
mg
GA
E/g
sam
ple
Total Phenolic Content
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Boiled: 0.67-21.08 mg GAE/g
Results
0
5
10
15
20
25B
itte
r G
ou
rd
Bo
ttle
Go
urd
Ch
ili P
ep
per
Ch
ine
se C
abb
age
Co
rn
Co
wp
ea
Eggp
lan
t
Gin
ger
Gre
en
Pe
pp
er
Jute
Mu
star
d
Okr
a
Squ
ash
Flo
we
r
Squ
ash
Fru
it
Spin
ach
Stri
ng
Be
ans
Swe
et
Po
tato
Taro
Sta
lk
Taro
Tu
be
r
Wat
er
Spin
ach
mg
GA
E/g
sam
ple
Total Phenolic Content
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Raw
Boiled
Results
0
5
10
15
20
25
30
35B
itte
r G
ou
rd
Bo
ttle
Go
urd
Ch
ili P
ep
per
Ch
ine
se C
abb
age
Co
rn
Co
wp
ea
Eggp
lan
t
Gin
ger
Gre
en
Pe
pp
er
Jute
Mu
star
d
Okr
a
Squ
ash
Flo
we
r
Squ
ash
Fru
it
Spin
ach
Stri
ng
Be
ans
Swe
et
Po
tato
Taro
Sta
lk
Taro
Tu
be
r
Wat
er
Spin
ach
mg
GA
E/g
sam
ple
Total Phenolic Content
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Summary: TPCTop Vegetables
Boiling generally induced up to 91% decrease
jute > eggplant > squash flower ≥ chili ≥
mustard ≥ chinese cabbage ≥ green pepper
Raw
Boiled
eggplant > green pepper > chili >
jute ≥ squash flower ˃ ginger
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Raw: 1.24 – 239.32 µmol TEq/g
Results
0
50
100
150
200
250
300B
itte
r G
ou
rd
Bo
ttle
Go
urd
Ch
ili P
ep
per
Ch
ine
se C
abb
age
Co
rn
Co
wp
ea
Eggp
lan
t
Gin
ger
Gre
en
Pe
pp
er
Jute
Mu
star
d
Okr
a
Oys
ter
Mu
shro
om
Squ
ash
Flo
we
r
Squ
ash
Fru
it
Spin
ach
Stri
ng
Be
ans
Swe
et
Po
tato
Taro
Sta
lk
Taro
Tu
be
r
Wat
er
Spin
ach
µm
ol T
Eq/g
sam
ple
DPPH Radical Scavenging Activity
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Boiled: 1.06 – 178.49 µmol TEq/g
Results
0
50
100
150
200
250B
itte
r G
ou
rd
Bo
ttle
Go
urd
Ch
ili P
ep
per
Ch
ine
se C
abb
age
Co
rn
Co
wp
ea
Eggp
lan
t
Gin
ger
Gre
en
Pe
pp
er
Jute
Mu
star
d
Okr
a
Oys
ter
Mu
shro
om
Squ
ash
Flo
we
r
Squ
ash
Fru
it
Spin
ach
Stri
ng
Be
ans
Swe
et
Po
tato
Taro
Sta
lk
Taro
Tu
be
r
Wat
er
Spin
ach
µm
ol T
Eq/g
sam
ple
DPPH Radical Scavenging Activity
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Raw
Boiled
Results
0
50
100
150
200
250
300B
itte
r G
ou
rd
Bo
ttle
Go
urd
Ch
ili P
ep
per
Ch
ine
se C
abb
age
Co
rn
Co
wp
ea
Eggp
lan
t
Gin
ger
Gre
en
Pe
pp
er
Jute
Mu
star
d
Okr
a
Oys
ter
Mu
shro
om
Squ
ash
Flo
we
r
Squ
ash
Fru
it
Spin
ach
Stri
ng
Be
ans
Swe
et
Po
tato
Taro
Sta
lk
Taro
Tu
be
r
Wat
er
Spin
ach
µm
ol T
Eq/g
sam
ple
DPPH Radical Scavenging Activity
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Top Vegetables
eggplant > green pepper > water spinach ≥ jute >chili ≥
cowpea ≥ okra ≥ sweet potato ≥ ginger
Boiled
jute > eggplant > water spinach > taro tuber ≥
green pepper ≥ ginger ≥ okra ≥ mustard
Raw
Boiling generally induced up to 81% decrease
Summary: DPPH
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Raw: 2.10 – 136.84 µmol TEq/g
Results
0
20
40
60
80
100
120
140
160B
itte
r G
ou
rd
Bo
ttle
Go
urd
Ch
ili
Ch
ine
se C
abb
age
Co
rn
Eggp
lan
t
Gre
en
Pe
pp
er
Okr
a
Squ
ash
Fru
it
Jute
Mu
star
d
Spin
ach
Up
lan
d K
angk
on
g
Co
wp
ea
Stri
ng
Be
ans
Swe
et
Po
tato
Taro
Tu
be
r
Gin
ger
Squ
ash
Flo
we
r
Taro
Sta
lk
Oys
ter
Mu
shro
om
µm
ol T
Eq /
g s
amp
le
ABTS Radical Cation Scavenging Activity
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Boiled: 1.06 – 178.49 µmol TEq/g
Results
0
10
20
30
40
50
60
70
80
90
100B
itte
r G
ou
rd
Bo
ttle
Go
urd
Ch
ili
Ch
ine
se C
abb
age
Co
rn
Eggp
lan
t
Gre
en
Pe
pp
er
Okr
a
Squ
ash
Fru
it
Jute
Mu
star
d
Spin
ach
Up
lan
d K
angk
on
g
Co
wp
ea
Stri
ng
Be
ans
Swe
et
Po
tato
Taro
Tu
be
r
Gin
ger
Squ
ash
Flo
we
r
Taro
Sta
lk
Oys
ter
Mu
shro
om
µm
ol T
Eq /
g s
amp
le
ABTS Radical Cation Scavenging Activity
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Raw
Boiled
Results
0
20
40
60
80
100
120
140
160B
itte
r G
ou
rd
Bo
ttle
Go
urd
Ch
ili
Ch
ine
se C
abb
age
Co
rn
Eggp
lan
t
Gre
en
Pe
pp
er
Okr
a
Squ
ash
Fru
it
Jute
Mu
star
d
Spin
ach
Up
lan
d K
angk
on
g
Co
wp
ea
Stri
ng
Be
ans
Swe
et
Po
tato
Taro
Tu
be
r
Gin
ger
Squ
ash
Flo
we
r
Taro
Sta
lk
Oys
ter
Mu
shro
om
µm
ol T
Eq /
g s
amp
le
ABTS Radical Cation Scavenging Activity
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Top Vegetables
eggplant ≥ green pepper >water spinach ≥
jute >chili ≥ cowpea
ginger >
Boiled
jute > eggplant > ginger > mustard ≥
green pepperpechay ≥
Raw
water spinach ≥
Boiling generally induced up to 85% decrease
Summary: ABTS
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Correlation AnalysisRaw Samples
r=0.931**
r=0.941**
0
50
100
150
200
250
300
0 10 20 30 40
An
tio
xid
ant
cap
acit
y (µ
mo
lTEq
/g s
amp
le)
TPC (mg GAE/g sample)
• DPPH • ABTS
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Correlation AnalysisCooked Samples
r=0.892**
r=0.828**
0
25
50
75
100
125
150
175
200
0 5 10 15 20 25
An
tio
xid
ant
Cap
acit
y (µ
mo
lTEq
/g s
amp
le)
TPC (mg GAE/g sample)
• DPPH • ABTS
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Conclusions
• Boiling generally reduced the TPC and antioxidantcapacities of the vegetables.
Jute Eggplant Water Spinach
Green Pepper Ginger
Top Vegetables
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Recommendations
• Consumption of raw or minimally heated vegetables isrecommended for optimum antioxidant intake.
• TPC is the main contributor for the antioxidantcapacities.
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Acknowledgement
• Mr. John Michael C. Avila
• Intensified Rice-Based Agri-Biosystems Program
• Future Rice Program
• United Nations University
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Thank you!
John Edward I. Zapater
Rice Chemistry and Food Science Division
Philippine Rice Research Institute
Email: [email protected]
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“Everything is marketing.”
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Rice-based Product Concepts with Health and Nutritional Value: The Experts’ Perspective on Consumer Trends
JFBallesteros, RGARamos, and RVManaois
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A need for market research
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time
fund
talent
facility
technology developer
Our situation
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• Value-adding
• Profitability
• Nutritional benefits
roduct Development
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The risk of market failure
38-55%of new products
FAIL
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The gapneeds
demandwants
timefund
talentfacility
Consumer knowledge
technology developer customers
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The biggest rule
Know Your
Market
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Objectives
Describe experts’ perception on health and wellness
Describe experts’ perception in the range of products available in the market
Generate rice-based product concepts
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What did we do?
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Focus Group Discussion (FGD)
2413
average years of experience
experts
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The Experts
6
6
7
2
2
1
1
Social entrepreneur, food technologists, managers, nutritionist-dietitians, researchers, chefs,
and business owners
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What we found out?
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FGD Outputs
Health and wellness
Rice-based product concepts considering health and nutrition
Consumer Trends
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28%
15%
11%11%
9%
6%
5%
5%
3%3%
3% 2%
Food, Diet and Nutrition Health Products Lifestyle
Quality of Life Exercise/Physical Activity Life Expectancy
Environment Diseases Beauty/Hygiene
Mental Health Family/Relationship Health care service
Health and Wellness
Top 5 thoughts
food, proper diet and nutrition
health products lifestyle views on quality of life exercise/physical activity
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Consumer Trends
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57%18%
8%
4%
3%
2%2%
6%
Restaurant/Fastfood Meals Home cooked Meat/Fish
Instant Meals Home cooked vegetable (Chopsuey)
Noodles Nachos/Tacos/Nuggets
Brown Rice Others
Fast food and restaurant meals
Home cooked meat/fish
Instant meals
Meals
Top 3
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23%
15%
15%
5%
5%
4%
4%
3%
3%
3%
3%
3%
3%
2%2%
2% 4%
Crackers and Chips Breads and doughnuts Burgers and sandwich
Biscuits and Cookies Non-packed snacks (banana cue/saba/taho) Pasta
Cakes and hotcakes Instant Noodles Restaurant Snacks (stew/nalongbao)
Pizzas Confectionery (chocolates and other sweets) Ice cream
Rice-based/kakanin Packed Cereals (Oat) Yoghurt
Others
Snacks
crackers and chips breads and
doughnuts burgers and
sandwiches
Top 3
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13%
11%
9%
8%7%
7%6%
6%
6%
5%
5%
4%
4%
3%2%
2%4%
RTD Coffee-based drinks Soft drinks RTD Fruit Juice
Alcoholic beverages RTD tea drinks Health drinks
RTD fermented drink (yoghurt) Energy drinks Flavored drinks (Mogu-mogu)
RTD milk-based drinks Natural/Fresh drinks (Buko juice) Bottled water (flavored and unflavored)
Smoothies and Shakes Powdered juice RTD Cereal drink
Choco-drink Others
ready-to-drink (RTD) coffee drinks
Soft drinks RTD fruit juices
Drinks/Beverages
Top 3
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19%
16%
13%
9%
8%
7%
4%
4%
3%
3%
3%
2%2%2%
2% 4%
Facial care products Body Moisturizer Soap and body wash Toothpaste
Hair colorant Shampoo and Conditioner Body Alcohol Feminine Wash
Sanitizer Deodorant and Perfume Baby Powder Mouth wash
Lip Balm/Lipstick Napkin Body scrub Other
facial care products body moisturizers soaps and body wash
Hygiene and beauty products
Top 3
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46%
29%
8%
5%
4%
3%3%1% 1%
Food supplements Vitamins/Multivitamins Fortified drinks
Over-the-counter drugs Ointments Pain reliever
Slimming capsules Whitening supplements Coconut oil
Pharmaceutical/Nutraceutical Products
vitamins/multivitamins
food supplements
fortified drinks
Top 3
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Rice-based product conceptsExperts suggest…
Personal care/hygiene products
Flour-based products
Drinks/beverages
46%
29%
8%
8% Special purpose products
7% Instant products
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Conclusions
Health and wellness is mostly associated with concepts on food, proper diet, and nutrition
Health
Convenience
Beauty
Key Consumer Trends
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Future works
consumer research (market survey)
product concept screening
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Acknowledgement
FGD Participants
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Thank you!
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EH Bandonill, MJC Ablaza, OC Soco, and GG Corpuz
COOKED RICE SPOILAGE AND PRACTICAL WAYS OF RETARDING IT
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Current Practice
• Cooking rice enough for the whole day
• Keeping cooked rice at ambient temperature
• Consumption of left over rice without reheating
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Challenges
• Spoilage of cooked rice
• Improper food handling
• Food-borne outbreaks (Lake et al. 2004)
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Challenges
Bacillus cereus – bacterium that survives heat while boiling; capable of growing in cooked rice
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Objectives
• Know the possible causes of spoilage in cooked rice
• Discover practical ways of retarding spoilage
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MethodologyAmylose content (AC) - gelatinization
temperature (GT) combination
Rice Variety AC-GT Texture
Koshihikari Low-Low Very soft
NSIC Rc160 Low Low Very Soft
NSIC Rc140 Int-Int Soft
NSIC Rc142 Int-Int Soft
NSIC Rc158 Int-Int Soft
PSB Rc72H Int-Low Medium Soft
NSIC340 High-Low Hard
PSB Rc10 High-Int Hard
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• Rice samples: IR64 and NSIC Rc160
• Rice ageing
2014 Dry Season (12 mo)
2014 Wet Season (6 mo)
2015 Dry Season (2 mo)
• Amount of rice cooked per batch
300 g vs 500 g
• Periodic heating
0, 12, 18, 24, 30, 36, 42 h
Methodology
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• Storage TemperatureRoom/Ambient (27-29oC; 50-65% RH)Refrigerated (4-8oC)
•Reheating PracticeUnreheatedMicrowaveSteaming
Methodology
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• Microbial Load (Total Plate Count, cfu/g)
• Sensory Index (SI)
parameters: color, odor, texturescale: 1 (unacceptable) to 3 (acceptable)SI = 2.C + 2.O + 1.T
5SI = 1.8 (spoiled)
Methodology
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0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
0h 12h 15h 18h 21h 24h
Sampling Time
Results: AC-GT Combination
High/Int AC-Low GT rice (medium soft to hard-textured) –higher sensory index (24h); tolerable microbial load
Sen
sory
In
dex
(SI
)
High/Int AC-Low GT
Low-Low/Int-Int AC-GT
Low-Low to Int-Int AC-GT (very soft to soft-textured) – lower SI (18h); high microbial load; slightly perceptible to perceptible off-odor
Storage Time
106-107 cfu/g
105 cfu/g
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0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
0h 15h 18h 21h 24h
Storage Time
Aged (6 and12 mo) – spoiled later at 18 h
Results: Ageing PeriodSe
nso
ry I
nd
ex (
SI)
Aged (2 mo) - spoiled earlier at 15 h
105 cfu/g
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0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
0h 15h 18h 21h 24h
Storage Time
IR64: 300 g – remained acceptable up to 21h
Results: Amount of Rice Cooked Per BatchSe
nso
ry I
nd
ex (
SI)
500 g – up to 18h
x
105 cfu/g
106-107 cfu/g
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0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
0h 12h 18h 24h 30h 36h 42h
Reheating Period
Reheated (every 6 h) – had extended sensory index up to 42h
Results: Periodic ReheatingSe
nso
ry I
nd
ex (
SI)
106 cfu/g
105 cfu/g
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1.0
1.5
2.0
2.5
3.0
3.5
Day 0 Day 1 Day 2 Day 3 Day 4
Storage Time
Refrigerated – acceptable SI up to 4 days
Results: Storage TemperatureSe
nso
ry I
nd
ex (
SI)
Room temperature – acceptable sensory index up to 1 day
TNTC
104 cfu/g
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Results: Reheating PracticeOff-odor
0.0
1.0
2.0
3.0
4.0
5.0
6.0
DAY 0 DAY 1 DAY 2 DAY 3 DAY 4
OFF-ODOR
Sen
sory
Sco
re
Room – high off-odor score; refrigerated - low to no off-odorRefrigerated (reheated/unreheated) – low to no off-
odor
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Reheated (Microwave/steaming) - maintained cohesiveness until Day 4
Results: Reheating PracticeCohesiveness
Sen
sory
Sco
re
0.0
2.0
4.0
6.0
8.0
10.0
12.0
DAY 0 DAY 1 DAY 2 DAY 3 DAY 4
COHESIVENESS
Unreheated – decreasing score for cohesiveness
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Results: Reheating PracticeMoistness
Sen
sory
Sco
re
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
DAY 0 DAY 1 DAY 2 DAY 3 DAY 4
MOISTNESS
Reheated (Microwave/steaming) - maintained moistness until Day 4
Unreheated – decreasing score for moistness
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SUMMARY
• Very soft to soft-textured and freshly harvested rice is more prone to spoilage than medium soft to hard-textured and aged sample
• Cooking bigger amounts of rice has more tendency to spoil
• Periodic reheating and storing cooked rice at refrigerated temperature can retard spoilage up to 42h and 4 days, respectively
• Reheating through microwave/steaming makes rice comparable with freshly cooked sample (NSIC Rc160)
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RECOMMENDATIONS
• Cook rice in smaller quantities at several times of the day to reduce storage
• After cooking, rice should be kept hot (not lower than 60oC)
• Keep (within 1-2 hours), left over rice inside the refrigerator and not under warm conditions
• Reheat left over rice to as high as 75oC• If possible, age rice for more than 2 months
prior to milling and cooking• Cook/consume rice with intermediate-low
AC-GT types (medium soft)
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SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
MCV Newingham1, M Halwart2, EA Abella3, EM Vera Cruz3 and TA Abella3
1Philippine Rice Research Institute2 Food and Agriculture Organization of the United Nations3 Central Luzon State University
[email protected]+639228699672/+639985108560
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[TITLE OF PRESENTATION]
Rationale:• Rice-based ecosystems provide habitat for a
wide range of aquatic organisms used as food
• Contribute a large share of the animalprotein and other nutrient intake of poorhouseholds in Southeast Asia
• Importance of freshwater aquatic resourcesin the food security of rural households isgenerally underestimated and undervalued
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
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[TITLE OF PRESENTATION]
• There is an observed rapiddecline in the populationsof edible aquatic fauna
• There is a need to intervenesince some species mayhave been lost before a fulldocumentation could bedone
• No baseline data or countrystats re: status of freshwateraquatic fauna used as food
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
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[TITLE OF PRESENTATION]
Objectives• Documentation and identification of
aquatic fauna and the strategiesused to acquire them
• Identify species that have been lostor have been introduced in thecommunity
• Identify perceived threats to theecosystem and check for remedialactions done locally to help sustainthe food resource.
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
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[TITLE OF PRESENTATION]
Sampling locations
• Sites: Central Luzon (4) and Ifugao (1)
• Time frame: September 2013 – December 2014
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
Batad Talampas Sta. Monica
Mabini Villa Cuizon
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[TITLE OF PRESENTATION]
Strategies to collect data• Participatory rural appraisal
• Confirmatory survey
• Identification of Species
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
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[TITLE OF PRESENTATION]
Q: What is still available in the
ricefields of Luzon?
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
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[TITLE OF PRESENTATION]
Pre-survey results:
• A total of 45 species were listed:
– 25 fishes
– 9 mollusks
– 5 crustaceans
– 1 insect
– 1 amphibian
– 4 reptiles
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
©Subin Pumsom
©Subin Pumsom
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[TITLE OF PRESENTATION]
Confirmatory survey results• 23/25 species of fishes documented
- 7/8 indigenous fish species found
Suswe or susay - Dermogenyspusilla
Lukaok or Martiniko - Anabas testudineus
Bulig or Dalag –Channa striata
Biya - Glossogobius giuris
Ayungin - Leiopotherapon
plumbeus
Kanduli - Arius sp. Hitong native - Clariasmacrocephalus
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
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[TITLE OF PRESENTATION]
- 16/17 introduced fish species found
Talandi or Burasi - Carassius gibelio
Buan-buan or Burasi - Carassiuscarassius auratus
Karpa or Burasi - Cyprinus carpio
Maya-mayang tabang -Hypophthalmichthys molitrix
Rohu - Labeo rohita
Dojo fish - Misgurnusanguillicaudatus
Bid-bid - Elops sp.
.
Tilapia - Oreochromis niloticus Tilapiang Tato - Vieja synspilum
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
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[TITLE OF PRESENTATION]
Guraming puti - Trichogaster sp.Guraming itim - Trichogaster pectoralis Guraming tuldok - Trichogastertricopterus
Hitong pula - Clarias batrachus Hitong Taiwan - Clarias gariepinus Janitor fish - Pterygoplichthys sp.
Kiwet - Monopterus albus
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
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[TITLE OF PRESENTATION]
Tulya - Corbicula fluminea Kuhol - Pila conica Golden Kuhol - Pomacea canaliculata
Susong bato - Angulyagra oxytropis Susong pilipit or Agurong - Melanoides sp.
• 5 species of mollusks (4 listed species not accounted)
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
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Hipong malaki - Macrobrachium idella Giant ulang - Macrobrachium rosenbergii Kuros - Metapenaeus ensis
Australian ulang - Cheraxquadricarinatus Talangkang bukid - Paratelphusa hydrodromus
• 5 species of crustaceans
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[TITLE OF PRESENTATION]
Palakang bukid - Fejervarya limnocharis
• 1 amphibian; 1 reptile (3 listed species not documented)
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
Pagong or Antipa - Cuora amboinensis
Sawa – Python reticulatus Bayawak – Varanus salvatorKobra / Ulupong – Najaphilippinensis
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Alukap – Lethocerus indicus
• 0 species of insects
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WANTED!!!Name: Alukap aka Giant Water Beetle
Alias: Obus
Length: approx. 2.5-3.0 inches
Whereabouts: Unknown
Latest Status: Missing in Action;
Probably Endangered or extinct in Central
Luzon and Ifugao?
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Manual gathering methods used
Hand gathering (manu-mano) Electrofishing (aparato) Rod or pole fishing (bingwit)
Spear gun (baril o pana) Scoop net (salok) Plunge basket (salakab)
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Manual gathering methods used
Sieve tray (yakayakan) Triangular frame sieve (sagat)
Rounded cast net (dala)
Small drag net (lambat) Large drag net (pukot)
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Installed traps
Twig trap (palamag) Bottle-shaped basket traps (bubo)
Screen trap (pangkolong) Large rectangular framed net trap (skylab)
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Installed traps
Stationary net trap (pante) Stationary funnel net trap (bocatot)
A B
C D
Mini framed net trap (mini-skylab)
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Determining species availability
Timeline (community perceptions)
Versus
Results of the confirmatory
survey
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
Scoring:4 = Abundant3 = Many2 = Few1 = Rare0 = No sighting / Endangered / Extinct?
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0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
Suswe / Susay Lukaok /Martiniko
Bulig / Dalag Biya Ayungin Kanduli Hitong native Igat
50 years ago(mid 1960s)
25 years ago(1990s)
2014
Indigenous species
Indigenous fish species availability as perceived by PRA participants
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
Ava
ilab
ility
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Introduced fish species availability as perceived by PRA participants
Ava
ilab
ility
Introduced species
0
0.5
1
1.5
2
2.5
3
3.5
4
4.5 50 years ago(mid 1960s)
25 years ago(1990s)
2014
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
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0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
50 years ago (mid1960s)
25 years ago (1990s)
2014
Ricefield-based mollusks availability as perceived by PRA participants
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
Ava
ilab
ility
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Ricefield-based crustacean species availability in as perceived by PRA participants
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
Ulang / HipongMalaki
Giant prawn /Parong blandi
Hipon / Kuros Australian ulang Talangkang bukid
50 years ago (mid 1960s)
25 years ago (1990s)
2014
Ava
ilab
ility
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0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
Palakang bukid Pagong / Antipa Kobra / Ulupong Sawa Bayawak
50 years ago (mid 1960s)
25 years ago (1990s)
2014
Ricefield-based amphibian and reptilian species availability as perceived by PRA participants
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
Ava
ilab
ility
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Threats as perceived by local residents
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
Land and water pollution
Land conversion to other activities
Water supply availability
Biological pollution due to alien/introduced species
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Conclusions• Identified 45 aquatic fauna as food resource; 36 existing
• Documented 18 methods (11 manual and 7 installed) ofacquiring food resource.– Electrofishing - most popular, most widely used and most devastating
• Documented 21 introduced; 9 “unaccounted” species
• Identified 4 threats as perceived by locals– Climate change was not seen as threat
– Remediation or control measures are almost non-existent at barrio level
– When asked to device or formulate control measures that may beimplemented, majority of the respondents said that the governmentshould do something about it.
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Endangered or extinct?
SURVEY OF AQUATIC FAUNA USED AS FOOD IN RICE-BASED ECOSYSTEMS IN LUZON
Igat Million fish Sulib Native kuhol Aggudong
Kuskusiling Ulupong Sawa Bayawak
“Ine” (2008-2013)
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Recommendations
• To address sustainability of aquatic food resources:– Nation-wide campaign to increase awareness of the dynamics in
the ricefield ecosystem starting at barangay level;
– Further studies in other locations to determine if the same threats are experienced and affecting the survival of these edible species;
– Development of farming practices that promote sustainability of this species;
– Total ban of electro-fishing
– Inter-agency collaborations to help conserve existing species.
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• To address threats:– Implementation of
• proper waste disposal policies;
• more stringent policies in handling inorganic chemicalsand a campaign for local awareness of the hazards ofinorganic chemical run-off;
• stricter land conversion policies;
• stricter policies in exotic species introduction andmanagement;
– Awareness campaign about climate change issues inthe barrios
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Recommendations
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Acknowledgements
Local residents of the participating barangays • FAO – FIRA • DOST-NSC •
CLSU / FAC, CAS-Bio, ICCEM • DA / BFAR, NIA, PhilRice, IPM-
KASAKALIKASAN • Dr. Jess Binamira • Dr. Rina Velasco • Ehna Lanuza • Mary Tauli • Mike De La Cruz • Bobby Punzal
• Celia Diaz
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Salamat po!!!
FISH BE WITH YOU &
HAVE A RICE DAY!!!