20 minutes analysis time

Available in Biological Fluids:Plasma, Serum, Urine, Cerebrospinal Fluid

Sensitive separation with Zivak® AA HPLC column

Deuterated internal standards

Approximately 2000 analysis with one column

Suitable for anyLC-MS/MS System

METABOLIC DISEASES

Main methods and procedures that have beenselected are based on EN ISO 13485 and 98/79/EC.

Accurate, sensitiverapid and quantitative

analysis of42 free amino acids

in a single runwithin 20 minutes

Properties of the analysis kit

Amino AcidsLC-MS/MS Analysis Kit in Biological Fluids

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ZIVAK TECHNOLOGIES Eski Besiktas Cad. No 44, 34400 ISTANBUL - TURKEY

Distribuido en España por:INGENIERIA ANALITICA S.L. Tel: (+34) 902.45.6677Fax: (+34) 902.46.6677www.ingenieria-analitica.cominf@ingenieria-analitica.com

METABOLIC DISEASES

Amino AcidsLC-MS/MS Analysis Kit in Biological Fluids

AnalyteLinearity

(R2)LOD

(µmol/L)LOQ

(µmol/L)Accuracy

(%)PrecisionIntra-Assay

(%CV)

PrecisionInter-Assay

(%CV)No

3-methyl-histidine (3-MeHIS)Alanine (ALA)Alpha-aminoadipic acid (AAA)Alpha-aminobutyric acid (ABA)Alpha-aminopimelic acid (APA)Anserine ( ANS)Arginine (ARG)Argininosuccinic acid (ARG-SUC)Asparagine (ASN)Aspartic Acid (ASP)Beta alanine (BALA)Beta-aminoisobutyric acid (BAIB)Citrulline (CIT)Cystathionine (CTH)Cysteine (CYS)Cystine (C-C)Gamma-aminobutyric acid (GABA)Glutamic Acid (GLU)Glutamine (GLN)Glycine (GLY)Histamine (HISTA)Histidine (HIS)Hydroxylysine (HYL)Hydroxyproline (HYP)Homocystine (HC-HC)Homocysteine (HCYS)Isoleucine (ILEU)Leucine (LEU)Lysine (LYS)Methionine (MET)1-methyl-histidine (1-MeHIS)Ornithine (ORN)Phenylalanine (PHE)Proline (PRO)Sarcosine (SAR)Serine (SER)Serotonin (SRTN)Thiaproline (THPR)Threonine (THR)Tryptophan (TRP)Tyrosine (TYR)Valine (VAL)

0.9940.9960.9940.9970.9850.9940.9840.9930.9970.9960.9850.9940.9840.9970.9930.9970.9950.9940.9980.9890.9860.9940.9970.9930.9850.9990.9960.9870.9940.9970.9930.9940.9940.9960.9850.9940.9910.9920.9940.9960.9930.997

1.990.781.500.831.201.651.252.100.870.660.662.331.172.320.830.353.301.330.991.650.832.300.662.100.320.250.831.250.331.940.831.940.330.871.260.601.332.090.780.650.850.95

6.572.574.952.743.965.454.136.932.872.182.187.693.867.662.740.8610.94.393.275.452.747.592.186.930.950.582.744.131.096.402.746.401.092.874.161.984.396.902.572.152.813.14

90.991.795.196.397.495.691.794.689.894.596.295.694.396.492.297.594.692.893.696.491.596.398.294.896.794.893.395.691.392.593.691.892.695.690.795.597.291.990.692.788.789.8

4.234.855.215.014.766.474.254.164.125.145.284.756.843.454.724.534.215.424.134.574.264.756.144.564.124.785.014.864.124.766.765.433.564.255.473.564.724.565.263.572.593.04

5.15.16.35.35.47.45.15.35.06.25.45.37.24.45.15.25.36.45.25.45.15.47.75.35.25.55.25.35.15.17.26.44.25.36.24.45.55.76.04.73.83.2

The test results have been validated with an AB Sciex API 4000 LC-MS/MS instrument in Nov. 2014.

Validation Results

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ZIVAK TECHNOLOGIES Eski Besiktas Cad. No 44, 34400 ISTANBUL - TURKEY