A study of immune status to HBV infection in health care workers in a tertiary health care centre
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patients. SNP typing was done based on PCR-Sequenc- ing and PCR-RFLP methods. To assess the Prevalence of single nucleotide polymorphisms (SNP) of the IL28B and its association with sustained virologic re- sponse (SVR) of patients with chronic hepatitis C to PEG-interferon/ ribavirin therapy. Methods: One hundred and fifty patients were enrolled in the present study. The blood DNA from peripheral blood was isolated and SNP typing was done by PCR-Sequencing and PCR-RFLP methods. Results and Conclusion: Genotyping of rs12979860 and rs8099917 by PCR-RFLP PCR-sequencing were concor- dant in 150 (100%) individuals. The sensitivity and specific- ity of the PCR-RFLP method for genotyping of both SNPs are 100%. Among these 150 patients with chronic hepatitis C, the frequency of the rs12979860 CC, CT and TT geno- types were 40.6%, 47.3% and 12.0% and the frequency of the rs8099917 TT, GT and GG genotypes were 59.3%, 34.6% and 6.0%, respectively. Also, two IL28B haplotypes (rs12979860-rs8099917) were found among our patients including C-T, and T-G with 64.3%, and 23.3% frequency, respectively. Corresponding author. Mohammed Nanne Khaja. E-mail: [email protected] ANTI HBS TITERS IN HEALTH CARE PROFESSIONALS AND STUDENTS AT UNIVERSITY HOSPITAL Sitaram Myneni, Naveen Mohandas, Shanthi Vijayaraghavan Sriramachandra Hospital, Chennai, India A Total of (n=953) Health care Professionals were included in this study. The group contained 831 (87%) doctors and nurses and 122 (13%) medical students.The total number of males were 317(33%) and females were 636(77%). Blood samples were collected and results analyzed. The protective antibody titers were defined as value greater than 10 MIu/ml. It was observed that 693 people (72%) of target population who were vaccinated with stan- dard three dose schedule had protective titers. Of 260 peo- ple (28%) of target group had low antibody titers (<10 Miu/ml) had either received incomplete dosage of vacci- nation or were unaware of their vaccination status. In this low titer group 43 patients got revaccinated and the rechecked titers were in protective range. In this study it has been observed that there were 20 people (2%) who had low titer value, less than 10miu/ml, even after vacci- nating for two consecutive times with standard three dose vaccination schedule. There were labeled as primary vaccine non responders. In this study 6 patients were ex- cluded as they were HBSAg positive. Conclusion: Checking of protective titre may be an effi- cient strategy in health Care professionals to identify non responders and to facilitate revaccination Corresponding author. Sitaram Myneni. E-mail: [email protected] PREVALANCE AND RISK FACTORS FOR DEVELOPMENT OF HEPATITIS B AND HEPATITIS C IN ESRD PATIENTS ON HEMODIALYSIS Harish Kumar Yedla, Y. Sreekanth, Shelley C. Paul, Venkata Reddy, Vishwanath Reddy, M. Harsha, K. Sridhar Department of Medical Gastroenterology, Narayana Medical College, Nellore, A.P, India Introduction: Prevalence of Hepatitis B & C among the patients on hemodialysis is variable. HBV and HCV infec- tion are important causes of morbidity and mortality in he- modialysis patient. Aim: Aim of the present study is to know the incidence and prevalence of Hepatitis B and Hepatitis C among the pa- tients with ESRD on hemodialysis. Methods: One hundred and fifity ESRD patients who are under regular hemodialysis from January 2012 at our insti- tute (Narayana Medical College –Nellore) are included in our study. Baseline HBsAg and anti HCV antibodies before dialysis and then after every 3 months are measured, using ELISA method. Liver function tests, renal function tests, hemoglobin are measured every month. Results: Prevalence and incidence of Hepatitis B are 6% (9/ 150) and 2.66% (4/150) respectively. Prevalence and Inci- dence of Hepatitis C are 9.33% (14/150), 8.66% (13/150) re- spectively. The most important risk factor for sero conversion is recurrent blood transfusion. Conclusion: The prevalence as well as incidence of Hepa- titis C is greater than those of Hepatitis B. The most impor- tant risk factors for sero conversion are multiple blood transfusions Corresponding author. Harish Kumar Yedla. E-mail: [email protected] A STUDY OF IMMUNE STATUS TO HBV INFECTION IN HEALTH CARE WORKERS IN A TERTIARY HEALTH CARE CENTRE Nayana Joshi, 1 Swapna Kulkarni, 1 Ajit Kumar, 1 V. Lakshmi, 2 Lavnya Vanjari, 2 Neeraja 2 1 Departments of Gastroenterology, Nizam's Institute of Medical Sciences, Hyderabad, India, 2 Departments of Microbiology, Nizam's Institute of Medical Sciences, Hyderabad, India ABSTRACTS 21 ST ANNUAL CONFERENCE —2013 S68 © 2013, INASL Viral Hepatitis
Transcript of A study of immune status to HBV infection in health care workers in a tertiary health care centre
ABSTRACTS 21ST ANNUAL CONFERENCE —2013
Vira
lHep
atitis
patients. SNP typing was done based on PCR-Sequenc-ing and PCR-RFLP methods. To assess the Prevalenceof single nucleotide polymorphisms (SNP) of theIL28B and its association with sustained virologic re-sponse (SVR) of patients with chronic hepatitis C toPEG-interferon/ ribavirin therapy.Methods:One hundred and fifty patients were enrolled inthe present study. The blood DNA from peripheral bloodwas isolated and SNP typing was done by PCR-Sequencingand PCR-RFLP methods.Results and Conclusion: Genotyping of rs12979860 andrs8099917 by PCR-RFLP PCR-sequencing were concor-dant in 150 (100%) individuals. The sensitivity and specific-ity of the PCR-RFLP method for genotyping of both SNPsare 100%. Among these 150 patients with chronic hepatitisC, the frequency of the rs12979860 CC, CT and TT geno-types were 40.6%, 47.3% and 12.0% and the frequency ofthe rs8099917 TT, GT and GG genotypes were 59.3%,34.6% and 6.0%, respectively. Also, two IL28B haplotypes(rs12979860-rs8099917) were found among our patientsincluding C-T, and T-G with 64.3%, and 23.3% frequency,respectively.
Corresponding author. Mohammed Nanne Khaja.E-mail: [email protected]
ANTI HBS TITERS IN HEALTH CAREPROFESSIONALS AND STUDENTS ATUNIVERSITY HOSPITAL
Sitaram Myneni, Naveen Mohandas,Shanthi Vijayaraghavan
Sriramachandra Hospital, Chennai, India
A Total of (n=953) Health care Professionals were includedin this study. The group contained 831 (87%) doctors andnurses and 122 (13%) medical students.The total numberof males were 317(33%) and females were 636(77%). Bloodsamples were collected and results analyzed.The protective antibody titers were defined as valuegreater than 10 MIu/ml. It was observed that 693 people(72%) of target population who were vaccinated with stan-dard three dose schedule had protective titers. Of 260 peo-ple (28%) of target group had low antibody titers (<10Miu/ml) had either received incomplete dosage of vacci-nation or were unaware of their vaccination status. Inthis low titer group 43 patients got revaccinated and therechecked titers were in protective range. In this study ithas been observed that there were 20 people (2%) whohad low titer value, less than 10miu/ml, even after vacci-nating for two consecutive times with standard threedose vaccination schedule. There were labeled as primaryvaccine non responders. In this study 6 patients were ex-cluded as they were HBSAg positive.
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Conclusion: Checking of protective titre may be an effi-cient strategy in health Care professionals to identify nonresponders and to facilitate revaccination
Corresponding author. Sitaram Myneni.E-mail: [email protected]
PREVALANCE AND RISK FACTORS FORDEVELOPMENT OF HEPATITIS B ANDHEPATITIS C IN ESRD PATIENTS ONHEMODIALYSIS
Harish Kumar Yedla, Y. Sreekanth, Shelley C. Paul,Venkata Reddy, Vishwanath Reddy, M. Harsha, K. Sridhar
Department of Medical Gastroenterology, Narayana Medical College,Nellore, A.P, India
Introduction: Prevalence of Hepatitis B & C among thepatients on hemodialysis is variable. HBV and HCV infec-tion are important causes of morbidity andmortality in he-modialysis patient.Aim: Aim of the present study is to know the incidence andprevalence of Hepatitis B and Hepatitis C among the pa-tients with ESRD on hemodialysis.Methods: One hundred and fifity ESRD patients who areunder regular hemodialysis from January 2012 at our insti-tute (Narayana Medical College –Nellore) are included inour study. Baseline HBsAg and anti HCV antibodies beforedialysis and then after every 3 months are measured, usingELISA method. Liver function tests, renal function tests,hemoglobin are measured every month.Results: Prevalence and incidence of Hepatitis B are 6% (9/150) and 2.66% (4/150) respectively. Prevalence and Inci-dence of Hepatitis C are 9.33% (14/150), 8.66% (13/150) re-spectively. The most important risk factor for seroconversion is recurrent blood transfusion.Conclusion: The prevalence as well as incidence of Hepa-titis C is greater than those of Hepatitis B. Themost impor-tant risk factors for sero conversion are multiple bloodtransfusions
Corresponding author. Harish Kumar Yedla.E-mail: [email protected]
A STUDY OF IMMUNE STATUS TO HBVINFECTION IN HEALTH CARE WORKERS INA TERTIARY HEALTH CARE CENTRE
Nayana Joshi,1 Swapna Kulkarni,1 Ajit Kumar,1
V. Lakshmi,2 Lavnya Vanjari,2 Neeraja2
1Departments of Gastroenterology, Nizam's Institute of MedicalSciences, Hyderabad, India, 2Departments of Microbiology, Nizam'sInstitute of Medical Sciences, Hyderabad, India
© 2013, INASL
JOURNAL OF CLINICAL AND EXPERIMENTAL HEPATOLOGY
ViralH
epatitis
Background and Aims: The Seroprevalence of anti HBsand the vaccination status amongst the health careworkers (HCW) at a tertiary care centre was studiedin353 HCW (83 doctors, 75 technicians, 132 nurses, 48workers and 5 office staff) A detailed H\o demography,past vaccination were noted.Results: There were 158 men and 195 women in age rangeof 18-65 years Anti HBs was tested using ELISA. The over-all anti HBs prevalence was 82.0%. Female and younger agehad higher anti HBs positivity. 296/353 (84%) gave a pasth/o vaccination and 88.5% vaccinees were anti Hbs +vewhile 11.5% were negative. Among the 41 HCW not vacci-nated 59% were anti HBs +ve, 27% had taken 3 doses while1 and 2 doses or only booster dose were taken by 7%, 12%and 9% of HCW. 11% were not sure of number of doses.The anti HBs was +ve in 64%, 90% and 96% in 1, 2 and 3doses. The anti Hbs titers > 500 mIU/ml was observed in44%.Conclusion: Majority (82%) of the HCW have protectiveanti HBs but still 19% were unprotected. The study sug-gests that in accidental exposure to HBV anti Hbs testingis cost saving &should be assessed before giving HBIG.
Corresponding author. Nayana Joshi.E-mail: [email protected]
A PROSPECTIVE STUDY OF PREVALENCE OFOCCULTHBV INFECTION ANDASSESSMENTOF RISK FACTORS FOR HBV TRANSMISSIONIN PERSONSWITHOCCULT HBV INFECTION
Shivaram Prasad Singh,1 Sudha Kumari Singh,2
Sanjib Kumar Kar,1 Bijay Misra,1
Manas Kumar Panigrahi,1 Debasis Misra1
1Department of Gastroenterology, S.C.B. Medical College, Cuttack,India, 2Kalinga Gastroenterology Foundation, Bajrakabati Road, Cuttack,India
Backgroundand aim:OccultHBV infection (OBI) is an im-portant cause of cryptogenic cirrhosis as well as activation ofHBV infection in immunosupressants. There is limited dataon prevalence of OBI and it's mode of transmission. Ouraim is to study the prevalence of OBI in general populationof coastal Odisha and find out the associated risk factors fortransmission of HBV in persons with occult HBV infection.Methods: From September 2010 to December 2012, consec-utive apparently healthy 173 volunteers were subjected toa questionnaire for different risk factors for HBV infection.First degree relatives of HBV infected patients and knowncases of chronic liver diseases were excluded.Results: Of 171 HBsAg negative volunteers, 151(87.3%)were males and 22(12.7%) females, mean age was39.2�10.6 years. Fifteen patients (8.7%) were anti-HBc pos-itive, of whom 9(5.3%) were HBV DNA positive by nested
Journal of Clinical and Experimental Hepatology | March 2013 | Vol. 3 | No
PCR. On evaluation of different risk factors, none hadpresent and/or previous history of abscess drainage, cir-cumcision or acupuncture. The prevalence of risk factorsin overall (171) andOBI (9) individuals were as follows: his-tory of jaundice (19/2; P =0.258), hospitalization(24/4; P=0.112), dental procedure (45/4; P =0.241), ear piercing(18/1; P =1.0), blood donation (51/2; P =1.0), exposure tocommunity barbers (39/5; P =0.028), promiscuous sexualhabit (15/3; P =0.032), childhood immunization underEPI (132/8; P =0.0688), HBV vaccination (43/5; P =0.043).On logistic regression analysis exposure to community bar-bers (C I= 0.009-0.165, P =0.029) and promiscuous sexualhabit (CI= 0.032-0.263, P =0.013) were independent predic-tors of occult HBV infection.Conclusion: The prevalence of Occult HBV infection incoastal Odisha was estimated to be 5.3%. Exposure to com-munity barbers and promiscuous sexual habit werestrongly associated with OBI; the role of other risk factorsfor transmission of OBI requires a large population study.The findings raise concerns regarding safety of blood do-nation by healthy volunteers with HBsAg screening alone.
Corresponding author. Shivaram Prasad Singh.E-mail: [email protected]
COMPARATIVE STUDY BETWEEN GREEKAND EGYPTIAN CHRONIC HCV, GENOTYPE4A, PATIENTS TREATED WITH PEGYLATEDINTERFERON AND RIBAVIRIN: THE ROLE OFETHNICITY AND OTHER PREDICTIVEFACTORS
Dimitrios Dimitroulopoulos,1 V. Papastergiou,2
L. Skorda,2 P. Lisgos,2 V. Ketikoglou,2 L. Kostas,2
P. Karatapanis2
1Gastroenterology Dpt, “Agios Savvas” Hospital, Athens, Greece, 21stInternal Medicine Dpt, General Hospital of Rhodes, Rhodes, Greece
Background: Hepatitis C virus genotype 4 (HCV-4) isspreading beyond Africa and the Middle East but data re-garding treatment with pegylated interferon alpha and ri-bavirin of European populations infected with HCV-4remains limited. Interestingly, European (vs. Egyptian) or-igin has been associated with lower sustained virologicalresponse rates.Aim: The aim of the study was to investigate the treatmentoutcomes of Greek (vs. Egyptian), treatment-naïve patientsinfected with HCV-4 (subtype a) and to identify factorsinfluencing response rates.Methods: One hundred seventy-seven consecutive pa-tients (mean age: 44.6�10.2, males: 143/177; 80.8%, Egyp-tians: 76/177; 42.9%) treated over a 7-year period at thehepatology clinics of three tertiary care hospitals in Greecewere retrospectively evaluated.
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