A Guide to Microscopy

The Light Microscope

Purpose: History

Flea glasses Anton van Leeuwenhoek Robert Hooke

Anton “The Great” Van Leeuwenhoek

Father of microscopy

Apprenticeship Created the first

light microscope

Robert Hooke

First to see cells

Observed cells in cork

The Compound Light Microscope

Uses 2 or more lenses to view a sample Objective lens Ocular lens

Light Source Advantages and Limitations

Parts of Interest

See homework for functions Diaphragm Revolving nose piece Course adjustment knob Fine adjustment knob Which knob to use during high

power?

Magnificaiton

Definition: Eye Piece: _____ Scanning Power: ____ Low Power: ____ High Power: ____ How do you calculate total

magnification?

Resolution

Definition:

Bad Microscope Good Microscope

Limitations of Light Microscope

Poor Magnification Poor Resolution WHY???

Stereomicroscope

Dissecting microscope: Why? Reflects light off of the surface Results in a ___ Image

Electron Microscopes

2 types: Transmission Electron Microscope

(TEM) Scanning Electron Microscope (SEM)

Electrons either pass through a specimen or bounce off of the surface to provide an image

Resolution and Magnification?

Images obtained from EM’s

Electron Microscopes

Advantages? Limitations?